HG10007696 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10007696
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionmetalloendoproteinase 1-like
LocationChr10: 10311475 .. 10312419 (+)
RNA-Seq ExpressionHG10007696
SyntenyHG10007696
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAATAAACCTTTGGTTCTTCTCTTTATCTCCATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGCTCCAAGTTTCTCCATTTGAATTTCTCAATGACCTCCAAGGATGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAACTTAAGAACTTTTTCTGTTATTACGGTTATTTAAACCATCGAACCAACGCCACTGGTCATCTGATCGAGGCTGACGTTGACGACACCTTCGACGATCTCCTCGAGTCAGCCATTAAAACCTACCAACAATACTTCCATCTTAGCCCCACTGGATTCCTGAATGCCGAGACGATATCCCAACTCGCAACACCTCGGTGCGGCGTTCCAGATATCGTCAATGGAACTACCGATCGAATGCTATCAGAACACAACGACAACGACGGTGATCATGATCACCATCACCACCACCTTCCTCACGTCGTCTCGCACTATGCCTTCTTCCCAGGAAGGCGCAGGTGGCCATCTACCAAATACCATCTCACCTACGCGTTTCTTCCCGGCACTCGAGCAGACGCGAAAGCACCGGTGGCTCGAGCGTTCGCGACGTGGGCTCGAAACACTCATTTTAAGTTTTCTTTGGCTACAAACTATAAAAGAGCTGACTTGAAGATTGGTTTCTATAGAGGAAACCATGGAGATGGATACCCATTTGATGGGCCAGGTGGGACTTTGGCACATGCATTTGCTCCAACTGATGGGAGATTTCATTATGATTCTACAGAGAAATGGGGAGTTGGGGCAGTGAGAGGGCGATATGATTTGCAAACGGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGACATAGCACTGTTAAGAATGCTATAATGTATCCTTATATCAATTCAGGGACTACAAAGGGTTTGAATGTTGATGATATCAAAGGAATCAAGGTTTTATACAATAGACACTAG

mRNA sequence

ATGGCAAATAAACCTTTGGTTCTTCTCTTTATCTCCATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGCTCCAAGTTTCTCCATTTGAATTTCTCAATGACCTCCAAGGATGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAACTTAAGAACTTTTTCTGTTATTACGGTTATTTAAACCATCGAACCAACGCCACTGGTCATCTGATCGAGGCTGACGTTGACGACACCTTCGACGATCTCCTCGAGTCAGCCATTAAAACCTACCAACAATACTTCCATCTTAGCCCCACTGGATTCCTGAATGCCGAGACGATATCCCAACTCGCAACACCTCGGTGCGGCGTTCCAGATATCGTCAATGGAACTACCGATCGAATGCTATCAGAACACAACGACAACGACGGTGATCATGATCACCATCACCACCACCTTCCTCACGTCGTCTCGCACTATGCCTTCTTCCCAGGAAGGCGCAGGTGGCCATCTACCAAATACCATCTCACCTACGCGTTTCTTCCCGGCACTCGAGCAGACGCGAAAGCACCGGTGGCTCGAGCGTTCGCGACGTGGGCTCGAAACACTCATTTTAAGTTTTCTTTGGCTACAAACTATAAAAGAGCTGACTTGAAGATTGGTTTCTATAGAGGAAACCATGGAGATGGATACCCATTTGATGGGCCAGGTGGGACTTTGGCACATGCATTTGCTCCAACTGATGGGAGATTTCATTATGATTCTACAGAGAAATGGGGAGTTGGGGCAGTGAGAGGGCGATATGATTTGCAAACGGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGACATAGCACTGTTAAGAATGCTATAATGTATCCTTATATCAATTCAGGGACTACAAAGGGTTTGAATGTTGATGATATCAAAGGAATCAAGGTTTTATACAATAGACACTAG

Coding sequence (CDS)

ATGGCAAATAAACCTTTGGTTCTTCTCTTTATCTCCATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGCTCCAAGTTTCTCCATTTGAATTTCTCAATGACCTCCAAGGATGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAACTTAAGAACTTTTTCTGTTATTACGGTTATTTAAACCATCGAACCAACGCCACTGGTCATCTGATCGAGGCTGACGTTGACGACACCTTCGACGATCTCCTCGAGTCAGCCATTAAAACCTACCAACAATACTTCCATCTTAGCCCCACTGGATTCCTGAATGCCGAGACGATATCCCAACTCGCAACACCTCGGTGCGGCGTTCCAGATATCGTCAATGGAACTACCGATCGAATGCTATCAGAACACAACGACAACGACGGTGATCATGATCACCATCACCACCACCTTCCTCACGTCGTCTCGCACTATGCCTTCTTCCCAGGAAGGCGCAGGTGGCCATCTACCAAATACCATCTCACCTACGCGTTTCTTCCCGGCACTCGAGCAGACGCGAAAGCACCGGTGGCTCGAGCGTTCGCGACGTGGGCTCGAAACACTCATTTTAAGTTTTCTTTGGCTACAAACTATAAAAGAGCTGACTTGAAGATTGGTTTCTATAGAGGAAACCATGGAGATGGATACCCATTTGATGGGCCAGGTGGGACTTTGGCACATGCATTTGCTCCAACTGATGGGAGATTTCATTATGATTCTACAGAGAAATGGGGAGTTGGGGCAGTGAGAGGGCGATATGATTTGCAAACGGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGACATAGCACTGTTAAGAATGCTATAATGTATCCTTATATCAATTCAGGGACTACAAAGGGTTTGAATGTTGATGATATCAAAGGAATCAAGGTTTTATACAATAGACACTAG

Protein sequence

MANKPLVLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGLNVDDIKGIKVLYNRH
Homology
BLAST of HG10007696 vs. NCBI nr
Match: XP_038880397.1 (metalloendoproteinase 1-like [Benincasa hispida])

HSP 1 Score: 598.2 bits (1541), Expect = 4.1e-167
Identity = 287/315 (91.11%), Postives = 295/315 (93.65%), Query Frame = 0

Query: 1   MANKPLV-LLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGY 60
           MANKPLV LLFISIFLPLCFSLPLLQVSPF FLNDLQGCKKGD+V GISKLKNFF YYGY
Sbjct: 1   MANKPLVILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYGY 60

Query: 61  LNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPD 120
           LNHR N TGHLIEAD DDTFDD LESAIKTYQQYFHL+PTG L+ ETISQLATPRCGVPD
Sbjct: 61  LNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVPD 120

Query: 121 IVNGTTDRMLSEH-NDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTR 180
           I+NGTT RMLSEH NDND +HDHHHHHLPH VSHYAFFPGRRRWPSTKY LTYAFLPGTR
Sbjct: 121 IINGTTSRMLSEHDNDNDSEHDHHHHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240
           ADAKAPVARAFATWARNTHFKFSLATNY+RADLKIGFY GNHGDGYPFDGPGGTLAHAFA
Sbjct: 181 ADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAFA 240

Query: 241 PTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKG 300
           PTDGRFHYDSTEKW VGAV+GRYDLQTVALHEIGHLLGLGHS V+NAIMYPYI SGTTKG
Sbjct: 241 PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTKG 300

Query: 301 LNVDDIKGIKVLYNR 314
           LN DDIKGIKVLYNR
Sbjct: 301 LNADDIKGIKVLYNR 315

BLAST of HG10007696 vs. NCBI nr
Match: XP_008443669.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo] >KAA0038230.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK30625.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 578.2 bits (1489), Expect = 4.4e-161
Identity = 277/314 (88.22%), Postives = 289/314 (92.04%), Query Frame = 0

Query: 1   MANKPL-VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGY 60
           M NK L +LLFIS+FLPLCFSLPLLQVSPF FLNDLQGCKKGDNVKGISKLKNFF YYGY
Sbjct: 1   MGNKTLAILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGY 60

Query: 61  LNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPD 120
           LNH+ NATGHLI+ D DDTFDD LE AIKTYQQYFHL+PTG LNAETISQLATPRCG PD
Sbjct: 61  LNHQINATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPD 120

Query: 121 IVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRA 180
           I+N +T RMLSEHN+ND  HD  HHHLPH VSHYAFFPGRRRWPSTKY LTYAFLPGTRA
Sbjct: 121 IINESTGRMLSEHNNNDSSHD--HHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRA 180

Query: 181 DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240
           DAKAPV RAFATWARNTHFKFSL TNY+RADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP
Sbjct: 181 DAKAPVTRAFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240

Query: 241 TDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGL 300
           TDGRFHYDSTEKW VGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYI SG+TKGL
Sbjct: 241 TDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGL 300

Query: 301 NVDDIKGIKVLYNR 314
           N DDIKGIKVLYNR
Sbjct: 301 NADDIKGIKVLYNR 312

BLAST of HG10007696 vs. NCBI nr
Match: XP_004139164.1 (metalloendoproteinase 3-MMP [Cucumis sativus] >KGN66657.1 hypothetical protein Csa_007261 [Cucumis sativus])

HSP 1 Score: 569.3 bits (1466), Expect = 2.0e-158
Identity = 276/315 (87.62%), Postives = 287/315 (91.11%), Query Frame = 0

Query: 1   MANKPL--VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYG 60
           M NKPL  +LL ISIFLPLCFSLPL+QVSPF FLNDLQGCKKGDNVKGISKLKNFF YYG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVP 120
           YLNH+ NATGHLI+ D +D FDD LESAIKTYQQYFHL+PTG LNAET+SQLATPRCG P
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 DIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTR 180
           DI+N TT RMLSE  DN   HD  HHHLPH VSHYAFFPGR RWPSTKY LTYAFLPGTR
Sbjct: 121 DIINETTGRMLSEDIDNVSSHD--HHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTR 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240
           ADAKAPVARAFATWARNTHFKF+L TNY+RADLKIGFYRGNHGDGYPFDGPGGTLAHAFA
Sbjct: 181 ADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240

Query: 241 PTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKG 300
           PTDGRFHYDSTEKW VGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYI SG+TKG
Sbjct: 241 PTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKG 300

Query: 301 LNVDDIKGIKVLYNR 314
           LNVDDIKGIKVLYNR
Sbjct: 301 LNVDDIKGIKVLYNR 313

BLAST of HG10007696 vs. NCBI nr
Match: XP_023516970.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 537.0 bits (1382), Expect = 1.1e-148
Identity = 264/319 (82.76%), Postives = 279/319 (87.46%), Query Frame = 0

Query: 1   MANKPLVLLFISIFLPLCFSLPLLQ------VSPFEFLNDLQGCKKGDNVKGISKLKNFF 60
           MANKPL+L F  IFLPLC SLPL +      VSPF FLNDLQGCKKGDNVKGISKLKNFF
Sbjct: 1   MANKPLILAFF-IFLPLCLSLPLPELPELPRVSPFAFLNDLQGCKKGDNVKGISKLKNFF 60

Query: 61  CYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPR 120
            YYGYLN RTN T ++   D +D FDD LE AIKTYQQYFHL+PTG LN ETISQLATPR
Sbjct: 61  HYYGYLNRRTNVTSYM--NDDNDVFDDHLEFAIKTYQQYFHLNPTGTLNPETISQLATPR 120

Query: 121 CGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFL 180
           CGVPDIVNGTT R+L EH+D+D D   HH+HLPHVVSHYAFFPG+RRWPS+KY LTYAFL
Sbjct: 121 CGVPDIVNGTTGRLLFEHDDDDDDDRVHHYHLPHVVSHYAFFPGKRRWPSSKYRLTYAFL 180

Query: 181 PGTRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLA 240
           P TR DAKAPV RAFATWAR THFKFSL TNYKRA+LKIGFY+GNHGDGYPFDGPGGTLA
Sbjct: 181 PSTRKDAKAPVTRAFATWARYTHFKFSLTTNYKRANLKIGFYKGNHGDGYPFDGPGGTLA 240

Query: 241 HAFAPTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSG 300
           HAFAPTDGRFHYDSTEKW VGAV+GRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYI SG
Sbjct: 241 HAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSG 300

Query: 301 TTKGLNVDDIKGIKVLYNR 314
           TTKGLN DDIKGIKVLYNR
Sbjct: 301 TTKGLNADDIKGIKVLYNR 316

BLAST of HG10007696 vs. NCBI nr
Match: KAG7023772.1 (Metalloendoproteinase 3-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 531.9 bits (1369), Expect = 3.6e-147
Identity = 265/322 (82.30%), Postives = 278/322 (86.34%), Query Frame = 0

Query: 1   MANKPLVLLFISIFLPLCFSLP---------LLQVSPFEFLNDLQGCKKGDNVKGISKLK 60
           MANKPL+L F  IFLPLC SLP         L QVSPF FLNDLQGCKKGDNVKGISKLK
Sbjct: 1   MANKPLILAFF-IFLPLCLSLPLPELPEWPELPQVSPFAFLNDLQGCKKGDNVKGISKLK 60

Query: 61  NFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLA 120
           NFF YYGYLNH  N TG+L   DVDD FDD LE AIKTYQ+YFHL+PTG LN ETISQLA
Sbjct: 61  NFFHYYGYLNHGINVTGYL--NDVDDVFDDPLEFAIKTYQRYFHLNPTGTLNPETISQLA 120

Query: 121 TPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTY 180
           TPRCGVPDIVNGTT R+L EH+D   D   H +HLPHVVSHYAFFPG+RRWPS+KY LTY
Sbjct: 121 TPRCGVPDIVNGTTGRLLFEHDD---DGHVHRYHLPHVVSHYAFFPGKRRWPSSKYRLTY 180

Query: 181 AFLPGTRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGG 240
           AFLPGTR DAKAPV RAFATWAR THFKFSL TNYKRA+LKIGFY+GNHGDGYPFDGPGG
Sbjct: 181 AFLPGTRKDAKAPVTRAFATWARYTHFKFSLTTNYKRANLKIGFYKGNHGDGYPFDGPGG 240

Query: 241 TLAHAFAPTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYI 300
           TLAHAFAPTDGRFHYDSTEKW VGAV+GRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYI
Sbjct: 241 TLAHAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYI 300

Query: 301 NSGTTKGLNVDDIKGIKVLYNR 314
            SGTTKGLN DDIKGIKVLYN+
Sbjct: 301 KSGTTKGLNADDIKGIKVLYNK 316

BLAST of HG10007696 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.3e-58
Identity = 122/293 (41.64%), Postives = 174/293 (59.39%), Query Frame = 0

Query: 29  FEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIK 88
           ++  ++  GC  G NV G+ ++K +F  +GY+             +  D FDD+L++A++
Sbjct: 41  WDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI-------PETFSGNFTDDFDDILKAAVE 100

Query: 89  TYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPH 148
            YQ  F+L+ TG L+A TI  +  PRCG PD+VNGT+  ++        + +    HL H
Sbjct: 101 LYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTS--LMHGGRRKTFEVNFSRTHL-H 160

Query: 149 VVSHYAFFPGRRRWPSTKYHLTYAFLPGT--RADAKAPVARAFATWARNTHFKFSLATNY 208
            V  Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   F+L+ ++
Sbjct: 161 AVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESF 220

Query: 209 KRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVG-------AVRG 268
             +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V  
Sbjct: 221 STSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTA 280

Query: 269 RYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
             DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 281 AVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of HG10007696 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 123/296 (41.55%), Postives = 168/296 (56.76%), Query Frame = 0

Query: 27  SPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESA 86
           +P+    +  GC  G    G+  LK +F ++GY+   TN +G+       D FDD+L++A
Sbjct: 41  NPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYIT-ETNLSGNF-----TDDFDDILKNA 100

Query: 87  IKTYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHL 146
           ++ YQ+ F L+ TG L+  T+  +  PRCG PD+VNGT+  M S     +          
Sbjct: 101 VEMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTS-TMHSGRKTFEVSFAGRGQRF 160

Query: 147 PHVVSHYAFFPGRRRWPSTKYHLTYAFLP--GTRADAKAPVARAFATWARNTHFKFSLAT 206
            H V HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   F+   
Sbjct: 161 -HAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVE 220

Query: 207 NYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKW--------GVGA 266
            +  +D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W        G  +
Sbjct: 221 RFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFIS 280

Query: 267 VRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           V    DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++G++ LY
Sbjct: 281 VSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of HG10007696 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 134/329 (40.73%), Postives = 182/329 (55.32%), Query Frame = 0

Query: 7   VLLFISIFLPLC--FSLPLLQVSPFEFLN----------DLQGCKKGDNVKGISKLKNFF 66
           +L+F     P+   F   +  + P +FLN           L GC  G+N+ G+SKLK +F
Sbjct: 8   ILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYF 67

Query: 67  CYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPR 126
             +GY+    N T         D FDD+L+SAI TYQ+ F+L  TG L++ T+ Q+  PR
Sbjct: 68  RRFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPR 127

Query: 127 CGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFL 186
           CG PD+++G ++        N G        +      Y+FFPG+ RWP  K  LTYAF 
Sbjct: 128 CGNPDLIDGVSEM-------NGG-------KILRTTEKYSFFPGKPRWPKRKRDLTYAFA 187

Query: 187 PGTRA--DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGT 246
           P      + K   +RAF  WA  T   F+ + +  RAD+ IGF+ G HGDG PFDG  GT
Sbjct: 188 PQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGT 247

Query: 247 LAHAFAPTDGRFHYDSTEKWGV--GAVRGR-------YDLQTVALHEIGHLLGLGHSTVK 306
           LAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS+V+
Sbjct: 248 LAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVE 307

Query: 307 NAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           +AIM+P I+ G  K  L  DDI+GI+ LY
Sbjct: 308 DAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of HG10007696 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 219.5 bits (558), Expect = 5.2e-56
Identity = 124/280 (44.29%), Postives = 158/280 (56.43%), Query Frame = 0

Query: 41  GDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTG 100
           G N KG+S +KN+F + GY+ +  +          DD FDD L SAIKTYQ+ ++L+ TG
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPH---------FDDNFDDTLVSAIKTYQKNYNLNVTG 107

Query: 101 FLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRR 160
             +  T+ Q+ TPRCGVPDI+  T                        ++S Y FF    
Sbjct: 108 KFDINTLKQIMTPRCGVPDIIINTNKTTSF-----------------GMISDYTFFKDMP 167

Query: 161 RWPSTKYHLTYAFLPGTRAD--AKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRG 220
           RW +    LTYAF P  R D   K+ +ARAF+ W    +  F   T+Y+ A++KI F   
Sbjct: 168 RWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASK 227

Query: 221 NHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVGA------VRGRYDLQTVALHEIG 280
           NHGD YPFDGPGG L HAFAPTDGR H+D+ E W          V   +DL++VA+HEIG
Sbjct: 228 NHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIG 287

Query: 281 HLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           HLLGLGHS+   AIMYP I   T K  L  DDI GI+ LY
Sbjct: 288 HLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

BLAST of HG10007696 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.5e-47
Identity = 113/283 (39.93%), Postives = 152/283 (53.71%), Query Frame = 0

Query: 41  GDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTG 100
           G +V G+S+LK +   +GY+N  +            D FD  LESAI  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEI--------FSDVFDGPLESAISLYQENLGLPITG 123

Query: 101 FLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRR 160
            L+  T++ ++ PRCGV       +D  ++ +ND             H  +HY +F G+ 
Sbjct: 124 RLDTSTVTLMSLPRCGV-------SDTHMTINND-----------FLHTTAHYTYFNGKP 183

Query: 161 RWPSTKYHLTYAFLPG------TRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIG 220
           +W   +  LTYA          T  D K    RAF+ W+      F    ++  ADLKIG
Sbjct: 184 KW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIG 243

Query: 221 FYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGV-----GAVRGRYDLQTVALH 280
           FY G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+     DL++VA H
Sbjct: 244 FYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATH 303

Query: 281 EIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           EIGHLLGLGHS+ ++A+MYP +   T K  L VDD+ G+  LY
Sbjct: 304 EIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of HG10007696 vs. ExPASy TrEMBL
Match: A0A5A7T4D6 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00410 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.1e-161
Identity = 277/314 (88.22%), Postives = 289/314 (92.04%), Query Frame = 0

Query: 1   MANKPL-VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGY 60
           M NK L +LLFIS+FLPLCFSLPLLQVSPF FLNDLQGCKKGDNVKGISKLKNFF YYGY
Sbjct: 1   MGNKTLAILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGY 60

Query: 61  LNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPD 120
           LNH+ NATGHLI+ D DDTFDD LE AIKTYQQYFHL+PTG LNAETISQLATPRCG PD
Sbjct: 61  LNHQINATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPD 120

Query: 121 IVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRA 180
           I+N +T RMLSEHN+ND  HD  HHHLPH VSHYAFFPGRRRWPSTKY LTYAFLPGTRA
Sbjct: 121 IINESTGRMLSEHNNNDSSHD--HHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRA 180

Query: 181 DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240
           DAKAPV RAFATWARNTHFKFSL TNY+RADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP
Sbjct: 181 DAKAPVTRAFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240

Query: 241 TDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGL 300
           TDGRFHYDSTEKW VGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYI SG+TKGL
Sbjct: 241 TDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGL 300

Query: 301 NVDDIKGIKVLYNR 314
           N DDIKGIKVLYNR
Sbjct: 301 NADDIKGIKVLYNR 312

BLAST of HG10007696 vs. ExPASy TrEMBL
Match: A0A1S3B8J8 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103487215 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.1e-161
Identity = 277/314 (88.22%), Postives = 289/314 (92.04%), Query Frame = 0

Query: 1   MANKPL-VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGY 60
           M NK L +LLFIS+FLPLCFSLPLLQVSPF FLNDLQGCKKGDNVKGISKLKNFF YYGY
Sbjct: 1   MGNKTLAILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGY 60

Query: 61  LNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPD 120
           LNH+ NATGHLI+ D DDTFDD LE AIKTYQQYFHL+PTG LNAETISQLATPRCG PD
Sbjct: 61  LNHQINATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPD 120

Query: 121 IVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRA 180
           I+N +T RMLSEHN+ND  HD  HHHLPH VSHYAFFPGRRRWPSTKY LTYAFLPGTRA
Sbjct: 121 IINESTGRMLSEHNNNDSSHD--HHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRA 180

Query: 181 DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240
           DAKAPV RAFATWARNTHFKFSL TNY+RADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP
Sbjct: 181 DAKAPVTRAFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240

Query: 241 TDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGL 300
           TDGRFHYDSTEKW VGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYI SG+TKGL
Sbjct: 241 TDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGL 300

Query: 301 NVDDIKGIKVLYNR 314
           N DDIKGIKVLYNR
Sbjct: 301 NADDIKGIKVLYNR 312

BLAST of HG10007696 vs. ExPASy TrEMBL
Match: A0A0A0M0J6 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G654860 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.9e-159
Identity = 276/315 (87.62%), Postives = 287/315 (91.11%), Query Frame = 0

Query: 1   MANKPL--VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYG 60
           M NKPL  +LL ISIFLPLCFSLPL+QVSPF FLNDLQGCKKGDNVKGISKLKNFF YYG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVP 120
           YLNH+ NATGHLI+ D +D FDD LESAIKTYQQYFHL+PTG LNAET+SQLATPRCG P
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 DIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTR 180
           DI+N TT RMLSE  DN   HD  HHHLPH VSHYAFFPGR RWPSTKY LTYAFLPGTR
Sbjct: 121 DIINETTGRMLSEDIDNVSSHD--HHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTR 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240
           ADAKAPVARAFATWARNTHFKF+L TNY+RADLKIGFYRGNHGDGYPFDGPGGTLAHAFA
Sbjct: 181 ADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240

Query: 241 PTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKG 300
           PTDGRFHYDSTEKW VGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYI SG+TKG
Sbjct: 241 PTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKG 300

Query: 301 LNVDDIKGIKVLYNR 314
           LNVDDIKGIKVLYNR
Sbjct: 301 LNVDDIKGIKVLYNR 313

BLAST of HG10007696 vs. ExPASy TrEMBL
Match: A0A6J1H9F9 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461758 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 6.6e-147
Identity = 265/325 (81.54%), Postives = 278/325 (85.54%), Query Frame = 0

Query: 1   MANKPLVLLFISIFLPLCFSLP------------LLQVSPFEFLNDLQGCKKGDNVKGIS 60
           MANKPL+L F  IFLPLC SLP            L QVSPF FLNDLQGCKKGDNVKGIS
Sbjct: 1   MANKPLILAFF-IFLPLCLSLPLPELPELPEWPELPQVSPFAFLNDLQGCKKGDNVKGIS 60

Query: 61  KLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETIS 120
           KLKNFF YYGYLNH  N TG+L   DVDD FDD LESAIKTYQ+YFHL+PTG LN ETIS
Sbjct: 61  KLKNFFHYYGYLNHGINVTGYL--NDVDDVFDDPLESAIKTYQRYFHLNPTGTLNLETIS 120

Query: 121 QLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYH 180
           QLATPRCGVPDIVNGTT R+L EH+D   D   H +HL HVVSHYAFFPG+RRWPS+KY 
Sbjct: 121 QLATPRCGVPDIVNGTTGRLLFEHDD---DGHVHRYHLSHVVSHYAFFPGKRRWPSSKYR 180

Query: 181 LTYAFLPGTRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDG 240
           LTYAFLPGTR DAKAPV RAFATWAR THFKFSL TNYKRA+LKIGFY+GNHGDGYPFDG
Sbjct: 181 LTYAFLPGTRKDAKAPVTRAFATWARYTHFKFSLTTNYKRANLKIGFYKGNHGDGYPFDG 240

Query: 241 PGGTLAHAFAPTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMY 300
           PGGTLAHAFAPTDGRFHYDSTEKW VGAV+GRYDLQTVALHEIGHLLGLGHSTVKNAIMY
Sbjct: 241 PGGTLAHAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVKNAIMY 300

Query: 301 PYINSGTTKGLNVDDIKGIKVLYNR 314
           PYI SGTTKGLN DDIKGIKVLYN+
Sbjct: 301 PYIKSGTTKGLNADDIKGIKVLYNK 319

BLAST of HG10007696 vs. ExPASy TrEMBL
Match: A0A5D3E491 (Metalloendoproteinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00480 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 4.0e-144
Identity = 252/314 (80.25%), Postives = 272/314 (86.62%), Query Frame = 0

Query: 1   MANKPLV-LLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGY 60
           MANKPLV LLFISIFLPLCFS PLL VSPF FLN LQGC KGDNVKGIS LKNFF +YGY
Sbjct: 1   MANKPLVILLFISIFLPLCFSTPLLHVSPFAFLNKLQGCTKGDNVKGISNLKNFFHHYGY 60

Query: 61  LNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPD 120
           LNH+ NAT HLI+ DVDDTFDD LESAIKTYQQYFHL+ TG LN ETISQLA PRCG PD
Sbjct: 61  LNHQINATSHLIDIDVDDTFDDRLESAIKTYQQYFHLNSTGSLNVETISQLAKPRCGNPD 120

Query: 121 IVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRA 180
           I++ T DRML EHN    D  H +HHLPH+VSH+ FF G RRWPSTKYHLTYAFLPGTR 
Sbjct: 121 IIDETADRMLLEHN---SDSRHDYHHLPHIVSHFTFFSGMRRWPSTKYHLTYAFLPGTRV 180

Query: 181 DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240
           D KAPVARAFATWAR THFKFSLA NY++A+LKIGFYRGNHGDGY FDGPG TLAH+FAP
Sbjct: 181 DVKAPVARAFATWARYTHFKFSLAKNYRKANLKIGFYRGNHGDGYSFDGPGRTLAHSFAP 240

Query: 241 TDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGL 300
           TDGRFHYDS EKW VGAVRGRYD+QTVALHEIGHLLGLGHS ++N+IMYPYI SG TKGL
Sbjct: 241 TDGRFHYDSAEKWTVGAVRGRYDMQTVALHEIGHLLGLGHSKIRNSIMYPYIKSGATKGL 300

Query: 301 NVDDIKGIKVLYNR 314
           ++DDI GIKVLY+R
Sbjct: 301 SLDDILGIKVLYHR 311

BLAST of HG10007696 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 226.9 bits (577), Expect = 2.3e-59
Identity = 122/293 (41.64%), Postives = 174/293 (59.39%), Query Frame = 0

Query: 29  FEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIK 88
           ++  ++  GC  G NV G+ ++K +F  +GY+             +  D FDD+L++A++
Sbjct: 41  WDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI-------PETFSGNFTDDFDDILKAAVE 100

Query: 89  TYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPH 148
            YQ  F+L+ TG L+A TI  +  PRCG PD+VNGT+  ++        + +    HL H
Sbjct: 101 LYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTS--LMHGGRRKTFEVNFSRTHL-H 160

Query: 149 VVSHYAFFPGRRRWPSTKYHLTYAFLPGT--RADAKAPVARAFATWARNTHFKFSLATNY 208
            V  Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   F+L+ ++
Sbjct: 161 AVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESF 220

Query: 209 KRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVG-------AVRG 268
             +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V  
Sbjct: 221 STSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTA 280

Query: 269 RYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
             DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 281 AVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of HG10007696 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 224.9 bits (572), Expect = 8.8e-59
Identity = 134/329 (40.73%), Postives = 182/329 (55.32%), Query Frame = 0

Query: 7   VLLFISIFLPLC--FSLPLLQVSPFEFLN----------DLQGCKKGDNVKGISKLKNFF 66
           +L+F     P+   F   +  + P +FLN           L GC  G+N+ G+SKLK +F
Sbjct: 8   ILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYF 67

Query: 67  CYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPR 126
             +GY+    N T         D FDD+L+SAI TYQ+ F+L  TG L++ T+ Q+  PR
Sbjct: 68  RRFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPR 127

Query: 127 CGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFL 186
           CG PD+++G ++        N G        +      Y+FFPG+ RWP  K  LTYAF 
Sbjct: 128 CGNPDLIDGVSEM-------NGG-------KILRTTEKYSFFPGKPRWPKRKRDLTYAFA 187

Query: 187 PGTRA--DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGT 246
           P      + K   +RAF  WA  T   F+ + +  RAD+ IGF+ G HGDG PFDG  GT
Sbjct: 188 PQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGT 247

Query: 247 LAHAFAPTDGRFHYDSTEKWGV--GAVRGR-------YDLQTVALHEIGHLLGLGHSTVK 306
           LAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS+V+
Sbjct: 248 LAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVE 307

Query: 307 NAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           +AIM+P I+ G  K  L  DDI+GI+ LY
Sbjct: 308 DAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of HG10007696 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 224.9 bits (572), Expect = 8.8e-59
Identity = 123/296 (41.55%), Postives = 168/296 (56.76%), Query Frame = 0

Query: 27  SPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESA 86
           +P+    +  GC  G    G+  LK +F ++GY+   TN +G+       D FDD+L++A
Sbjct: 41  NPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYIT-ETNLSGNF-----TDDFDDILKNA 100

Query: 87  IKTYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHL 146
           ++ YQ+ F L+ TG L+  T+  +  PRCG PD+VNGT+  M S     +          
Sbjct: 101 VEMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTS-TMHSGRKTFEVSFAGRGQRF 160

Query: 147 PHVVSHYAFFPGRRRWPSTKYHLTYAFLP--GTRADAKAPVARAFATWARNTHFKFSLAT 206
            H V HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   F+   
Sbjct: 161 -HAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVE 220

Query: 207 NYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKW--------GVGA 266
            +  +D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W        G  +
Sbjct: 221 RFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFIS 280

Query: 267 VRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           V    DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++G++ LY
Sbjct: 281 VSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of HG10007696 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 189.1 bits (479), Expect = 5.3e-48
Identity = 113/283 (39.93%), Postives = 152/283 (53.71%), Query Frame = 0

Query: 41  GDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTG 100
           G +V G+S+LK +   +GY+N  +            D FD  LESAI  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEI--------FSDVFDGPLESAISLYQENLGLPITG 123

Query: 101 FLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRR 160
            L+  T++ ++ PRCGV       +D  ++ +ND             H  +HY +F G+ 
Sbjct: 124 RLDTSTVTLMSLPRCGV-------SDTHMTINND-----------FLHTTAHYTYFNGKP 183

Query: 161 RWPSTKYHLTYAFLPG------TRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIG 220
           +W   +  LTYA          T  D K    RAF+ W+      F    ++  ADLKIG
Sbjct: 184 KW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIG 243

Query: 221 FYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGV-----GAVRGRYDLQTVALH 280
           FY G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+     DL++VA H
Sbjct: 244 FYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATH 303

Query: 281 EIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           EIGHLLGLGHS+ ++A+MYP +   T K  L VDD+ G+  LY
Sbjct: 304 EIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of HG10007696 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 166.4 bits (420), Expect = 3.7e-41
Identity = 106/277 (38.27%), Postives = 136/277 (49.10%), Query Frame = 0

Query: 47  ISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAET 106
           I ++K     YGYL           E+D     D   E A+  YQ+   L  TG  +++T
Sbjct: 50  IPEIKRHLQQYGYLPQNK-------ESD-----DVSFEQALVRYQKNLGLPITGKPDSDT 109

Query: 107 ISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWP-ST 166
           +SQ+  PRCG PD V   T                      H    Y +FPGR RW    
Sbjct: 110 LSQILLPRCGFPDDVEPKTAPF-------------------HTGKKYVYFPGRPRWTRDV 169

Query: 167 KYHLTYAFLPGTRADAKAPV------ARAFATWARNTHFKFSLATNYKRADLKIGFYRGN 226
              LTYAF         AP        RAF  WA      F    +Y  AD+KIGF+ G+
Sbjct: 170 PLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGD 229

Query: 227 HGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVGAVRGR----YDLQTVALHEIGHLL 286
           HGDG PFDG  G LAH F+P +GR H D  E W V     +     DL++VA+HEIGH+L
Sbjct: 230 HGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVL 289

Query: 287 GLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           GLGHS+VK+A MYP +   + K  LN+DD+ G++ LY
Sbjct: 290 GLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880397.14.1e-16791.11metalloendoproteinase 1-like [Benincasa hispida][more]
XP_008443669.14.4e-16188.22PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo] >KAA0038230.1 metallo... [more]
XP_004139164.12.0e-15887.62metalloendoproteinase 3-MMP [Cucumis sativus] >KGN66657.1 hypothetical protein C... [more]
XP_023516970.11.1e-14882.76metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo][more]
KAG7023772.13.6e-14782.30Metalloendoproteinase 3-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
Match NameE-valueIdentityDescription
O045293.3e-5841.64Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q5XF511.2e-5741.55Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
Q9ZUJ51.2e-5740.73Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
P291365.2e-5644.29Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
O235077.5e-4739.93Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7T4D62.1e-16188.22Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B8J82.1e-16188.22metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103487215 PE=3 SV... [more]
A0A0A0M0J69.9e-15987.62ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G654860 PE=3 S... [more]
A0A6J1H9F96.6e-14781.54metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461758 PE=3 ... [more]
A0A5D3E4914.0e-14480.25Metalloendoproteinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
AT1G70170.12.3e-5941.64matrix metalloproteinase [more]
AT1G59970.18.8e-5940.73Matrixin family protein [more]
AT1G24140.18.8e-5941.55Matrixin family protein [more]
AT4G16640.15.3e-4839.93Matrixin family protein [more]
AT2G45040.13.7e-4138.27Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 299..312
score: 51.22
coord: 209..237
score: 63.08
coord: 110..123
score: 50.7
coord: 185..200
score: 33.23
coord: 266..291
score: 58.26
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 158..313
e-value: 2.8E-33
score: 126.5
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 38..314
e-value: 2.4E-73
score: 248.6
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 162..311
e-value: 3.3E-45
score: 153.8
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 57..110
e-value: 7.8E-8
score: 32.4
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 9..312
NoneNo IPR availablePANTHERPTHR10201:SF269METALLOENDOPROTEINASE 3-MMP-LIKEcoord: 9..312
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 155..312
IPR021158Peptidase M10A, cysteine switch, zinc binding sitePROSITEPS00546CYSTEINE_SWITCHcoord: 113..120
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 161..311
e-value: 2.13029E-61
score: 190.878
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 39..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10007696.1HG10007696.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity