HG10007543 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10007543
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionisoleucine N-monooxygenase 1-like
LocationChr10: 6781177 .. 6781992 (-)
RNA-Seq ExpressionHG10007543
SyntenyHG10007543
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCTGCTTTCTTGTTCATTAGTCAATTGGCATATCAAAGTTCCTCAATAGCCATATCCAAAACTTTCATTGTGTTTGTATTTATGGCTATCATTTCTGTCATGTTTTCCCAATGTTATAACCTCAAAAGAAAACACTCAGTTCGACGACAAGCACCTCTCCCACCAGGCCCAAAGCCATGGCCACTCGTGGGTTGCCTCCCAACAATGTTAACCAACAAATCTCCAACACACCAATGGATTCATTCCATAATGAAGCAATTCAACACTGAAATTGCTTCTATACGCCTTGGAAATACTTACATAATACCTATCACTTCTCCAGAACTTACTCTTCAATTTCTCAAGACATATGATTCTGTCTTTGCCTCACGTTCTTCCATTTCCAACACCGTTGATATCTTAAGTCGTGGATATCTTACCACAGCATTTTCGTCTGTGGGAGACCAATGGAAGAAGATGAGAAGAATTCTCGCGTCGGAAATACTTAATCCGACAACGCTTCATCGAATGCTTGCCCAAAGAACCGCTGAAGCTGACACTCTTTTACGCTATATTTTCACCCAAACTAGTAAAAATGGAGGAGGTGCAATGATTAATGTTAGAAGCATAACACAACACTATTGTGGTAACATTATCCGAAGAATGCTATTCAACAAAAGATACTATGGTGAAGGGAGGGAAGATGGCGGGCCAACTTTTGAAGAAGAAGATCATAATCAAGCATTGTTGACCATTCTTAGGCATGTTAATGCATTTTCTATATCAGATTTCATGCCTTGTTTGAAGCCATTTGATATAAGATGGCCATGA

mRNA sequence

ATGGAAGCTGCTTTCTTGTTCATTAGTCAATTGGCATATCAAAGTTCCTCAATAGCCATATCCAAAACTTTCATTGTGTTTGTATTTATGGCTATCATTTCTGTCATGTTTTCCCAATGTTATAACCTCAAAAGAAAACACTCAGTTCGACGACAAGCACCTCTCCCACCAGGCCCAAAGCCATGGCCACTCGTGGGTTGCCTCCCAACAATGTTAACCAACAAATCTCCAACACACCAATGGATTCATTCCATAATGAAGCAATTCAACACTGAAATTGCTTCTATACGCCTTGGAAATACTTACATAATACCTATCACTTCTCCAGAACTTACTCTTCAATTTCTCAAGACATATGATTCTGTCTTTGCCTCACGTTCTTCCATTTCCAACACCGTTGATATCTTAAGTCGTGGATATCTTACCACAGCATTTTCGTCTGTGGGAGACCAATGGAAGAAGATGAGAAGAATTCTCGCGTCGGAAATACTTAATCCGACAACGCTTCATCGAATGCTTGCCCAAAGAACCGCTGAAGCTGACACTCTTTTACGCTATATTTTCACCCAAACTAGTAAAAATGGAGGAGGTGCAATGATTAATGTTAGAAGCATAACACAACACTATTGTGGTAACATTATCCGAAGAATGCTATTCAACAAAAGATACTATGGTGAAGGGAGGGAAGATGGCGGGCCAACTTTTGAAGAAGAAGATCATAATCAAGCATTGTTGACCATTCTTAGGCATGTTAATGCATTTTCTATATCAGATTTCATGCCTTGTTTGAAGCCATTTGATATAAGATGGCCATGA

Coding sequence (CDS)

ATGGAAGCTGCTTTCTTGTTCATTAGTCAATTGGCATATCAAAGTTCCTCAATAGCCATATCCAAAACTTTCATTGTGTTTGTATTTATGGCTATCATTTCTGTCATGTTTTCCCAATGTTATAACCTCAAAAGAAAACACTCAGTTCGACGACAAGCACCTCTCCCACCAGGCCCAAAGCCATGGCCACTCGTGGGTTGCCTCCCAACAATGTTAACCAACAAATCTCCAACACACCAATGGATTCATTCCATAATGAAGCAATTCAACACTGAAATTGCTTCTATACGCCTTGGAAATACTTACATAATACCTATCACTTCTCCAGAACTTACTCTTCAATTTCTCAAGACATATGATTCTGTCTTTGCCTCACGTTCTTCCATTTCCAACACCGTTGATATCTTAAGTCGTGGATATCTTACCACAGCATTTTCGTCTGTGGGAGACCAATGGAAGAAGATGAGAAGAATTCTCGCGTCGGAAATACTTAATCCGACAACGCTTCATCGAATGCTTGCCCAAAGAACCGCTGAAGCTGACACTCTTTTACGCTATATTTTCACCCAAACTAGTAAAAATGGAGGAGGTGCAATGATTAATGTTAGAAGCATAACACAACACTATTGTGGTAACATTATCCGAAGAATGCTATTCAACAAAAGATACTATGGTGAAGGGAGGGAAGATGGCGGGCCAACTTTTGAAGAAGAAGATCATAATCAAGCATTGTTGACCATTCTTAGGCATGTTAATGCATTTTCTATATCAGATTTCATGCCTTGTTTGAAGCCATTTGATATAAGATGGCCATGA

Protein sequence

MEAAFLFISQLAYQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGPKPWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYDSVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEADTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEEDHNQALLTILRHVNAFSISDFMPCLKPFDIRWP
Homology
BLAST of HG10007543 vs. NCBI nr
Match: XP_038878794.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 483.4 bits (1243), Expect = 1.3e-132
Identity = 238/268 (88.81%), Postives = 253/268 (94.40%), Query Frame = 0

Query: 1   MEAAFLFISQLAYQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGPK 60
           MEAAFL ISQLAYQ+S IAISKTFIVFVFMA ISV+FS CYNLK K SVRR+APLPPGPK
Sbjct: 1   MEAAFLIISQLAYQNSLIAISKTFIVFVFMAFISVIFSHCYNLKTKRSVRRKAPLPPGPK 60

Query: 61  PWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYD 120
           PWPLVGCLPTMLT K P HQWIHS+MKQFNTEIASIRLGNTYIIP+TSPEL LQFLK YD
Sbjct: 61  PWPLVGCLPTMLTIKYPPHQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKIYD 120

Query: 121 SVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEA 180
           S+FASRSSISNTVDILSRGYLTTAFSS+GDQW+KMRRILASEILNP  LHRML QRT+EA
Sbjct: 121 SIFASRSSISNTVDILSRGYLTTAFSSMGDQWRKMRRILASEILNPKMLHRMLGQRTSEA 180

Query: 181 DTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEEDH 240
           D+LLRYIFT TSKNGGGA+INVRSITQHYCGNIIRRMLFN+RYYG+GREDGGPTFEEEDH
Sbjct: 181 DSLLRYIFTLTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEDH 240

Query: 241 NQALLTILRHVNAFSISDFMPCLKPFDI 269
           NQALLTILRHVNAFSISDF+PCLKPFD+
Sbjct: 241 NQALLTILRHVNAFSISDFVPCLKPFDL 268

BLAST of HG10007543 vs. NCBI nr
Match: XP_038880015.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 465.7 bits (1197), Expect = 2.7e-127
Identity = 231/268 (86.19%), Postives = 252/268 (94.03%), Query Frame = 0

Query: 1   MEAAFLFISQLAYQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGPK 60
           MEAAFLFISQLA+Q S IAI+KTFIVFVFMA  SV+FS  YN +RK SV +QAPLPPGPK
Sbjct: 1   MEAAFLFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSV-QQAPLPPGPK 60

Query: 61  PWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYD 120
           PWPLVGCLPT+LTNKSPT+QWIHS+MKQFNTEIASIRLGNTYIIP+TSPEL LQFLKTYD
Sbjct: 61  PWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYD 120

Query: 121 SVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEA 180
           S+FASRSSISNTVDILSRGYLTTAFSS+G+QWKKMRRILASE+LNPT+LH+ML QRTAEA
Sbjct: 121 SIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEA 180

Query: 181 DTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEEDH 240
           D LL YIF  TSKNGGGA+INVRSITQHYCGNIIRRMLFN+RYYG+GREDGGPT EEEDH
Sbjct: 181 DNLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDH 240

Query: 241 NQALLTILRHVNAFSISDFMPCLKPFDI 269
           N+ALLTILRHVNAFSISDF+PCLKPFD+
Sbjct: 241 NEALLTILRHVNAFSISDFVPCLKPFDL 267

BLAST of HG10007543 vs. NCBI nr
Match: XP_008450367.2 (PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis melo])

HSP 1 Score: 451.4 bits (1160), Expect = 5.3e-123
Identity = 222/269 (82.53%), Postives = 251/269 (93.31%), Query Frame = 0

Query: 1   MEAAF-LFISQLAYQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGP 60
           MEAAF +FI+QL +Q+S IAISK FIVF+FMA  SVMFS+CYN ++K+SVR+ A LPPGP
Sbjct: 1   MEAAFSIFITQLPHQNSPIAISKAFIVFLFMAFASVMFSRCYNRRKKYSVRK-ALLPPGP 60

Query: 61  KPWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTY 120
           KPWP+VGCLPTMLTNKSPT+QWIHS+MKQFNTEIA IRLGNTY+IP+TSPEL L+FL+T+
Sbjct: 61  KPWPIVGCLPTMLTNKSPTYQWIHSVMKQFNTEIACIRLGNTYVIPVTSPELALEFLRTH 120

Query: 121 DSVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAE 180
           DSVF+SRSSISNTVDILSRGYLTTAFSS+GDQWKKMRRILASEIL+PT LHRML QR AE
Sbjct: 121 DSVFSSRSSISNTVDILSRGYLTTAFSSMGDQWKKMRRILASEILSPTMLHRMLEQRIAE 180

Query: 181 ADTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEED 240
           ADTLL YIF+ TS+NGGGA+INVRSITQHYCGNIIR MLFN+RYYG+GREDGGPT EEE+
Sbjct: 181 ADTLLHYIFSITSENGGGAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTSEEEE 240

Query: 241 HNQALLTILRHVNAFSISDFMPCLKPFDI 269
           HNQALLTILRH+NAFSISDFMPCLKPFD+
Sbjct: 241 HNQALLTILRHINAFSISDFMPCLKPFDL 268

BLAST of HG10007543 vs. NCBI nr
Match: XP_004151661.2 (isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8653524.1 hypothetical protein Csa_007554 [Cucumis sativus])

HSP 1 Score: 443.7 bits (1140), Expect = 1.1e-120
Identity = 219/269 (81.41%), Postives = 244/269 (90.71%), Query Frame = 0

Query: 1   MEAAFL-FISQLAYQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGP 60
           MEA FL F++QL +Q S IAISKTFI+F+FMA  SVMFS  YN ++K+SV R+  LPPGP
Sbjct: 1   MEAGFLIFLTQLTHQHSPIAISKTFIIFLFMAFASVMFSHYYNRRKKYSVVRKPRLPPGP 60

Query: 61  KPWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTY 120
           KPWP+VGCLPTMLTNKSPT  WIHS+MKQFNTEIA IRLG+TYIIP+TSPEL ++FLKT+
Sbjct: 61  KPWPIVGCLPTMLTNKSPTSHWIHSVMKQFNTEIACIRLGSTYIIPVTSPELAIEFLKTH 120

Query: 121 DSVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAE 180
           DSVF+SRSSISNTV ILSRGYLTTAFSS+GDQWKKMRRILASEILNPT LH+ML QRTAE
Sbjct: 121 DSVFSSRSSISNTVHILSRGYLTTAFSSMGDQWKKMRRILASEILNPTMLHQMLGQRTAE 180

Query: 181 ADTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEED 240
           ADTLLRYIF+ TS NGG A+INVRSITQHYCGNIIR MLFN+RYYG+GREDGGPT EEE+
Sbjct: 181 ADTLLRYIFSITSGNGGSAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTLEEEE 240

Query: 241 HNQALLTILRHVNAFSISDFMPCLKPFDI 269
           HNQALLTILRHVNAFSISDFMPCLKPFD+
Sbjct: 241 HNQALLTILRHVNAFSISDFMPCLKPFDL 269

BLAST of HG10007543 vs. NCBI nr
Match: XP_038880438.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 427.9 bits (1099), Expect = 6.3e-116
Identity = 211/270 (78.15%), Postives = 242/270 (89.63%), Query Frame = 0

Query: 1   MEAAFLFISQLAYQSSSIAI-SKTFIVFVFMAIISVMFSQCY-NLKRKHSVRRQAPLPPG 60
           M  AFLFI QLA+Q+S IAI SKT IVF FMA+ S++FSQCY N +RKH VRR  PLPPG
Sbjct: 1   MVIAFLFIPQLAHQNSPIAITSKTLIVFGFMALTSILFSQCYCNRRRKHLVRR-PPLPPG 60

Query: 61  PKPWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKT 120
           PKPWPLVGCLPTML NKSPTHQWIHS+MK+ NTEIASIRLGNTY+IP+TSPEL L+FLKT
Sbjct: 61  PKPWPLVGCLPTMLINKSPTHQWIHSVMKKLNTEIASIRLGNTYVIPVTSPELALEFLKT 120

Query: 121 YDSVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTA 180
           YDSVFASRSS SN + IL+RGY++T  S +GDQWKKMRRIL S+ILNP+TLHRML QRTA
Sbjct: 121 YDSVFASRSSTSNAIYILTRGYVSTILSPLGDQWKKMRRILTSKILNPSTLHRMLGQRTA 180

Query: 181 EADTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEE 240
           EADTLLRYIFTQT+ N GGA+IN+RSITQHYCGNI+R+MLFN+RYYG+GREDGGPTFEEE
Sbjct: 181 EADTLLRYIFTQTNNNRGGAVINLRSITQHYCGNILRQMLFNRRYYGKGREDGGPTFEEE 240

Query: 241 DHNQALLTILRHVNAFSISDFMPCLKPFDI 269
           +H QALLTI++H++AFSISDFMPCLK FD+
Sbjct: 241 EHVQALLTIVKHIHAFSISDFMPCLKSFDL 269

BLAST of HG10007543 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 6.2e-66
Identity = 130/268 (48.51%), Postives = 182/268 (67.91%), Query Frame = 0

Query: 1   MEAAFLFISQLAYQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGPK 60
           M   +LF+   +  +  I++S  FI+F+F+    V F        K+SV    PLPPGP 
Sbjct: 1   MSLPYLFLD--SEVTPPISLSLAFIIFMFL----VKF--ILKTHNKNSV-PVVPLPPGPS 60

Query: 61  PWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYD 120
           PWP+VG LP M  N+ P H+WIHS+MK+ NT+IA IRLGN ++IP+TSPE+  +FLK  D
Sbjct: 61  PWPIVGSLPEMWRNR-PAHRWIHSLMKKLNTDIACIRLGNVHVIPVTSPEIAREFLKKND 120

Query: 121 SVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEA 180
           +VFASR  ++     LS GYLTT     GDQW+KMRR+L +E  NP+ +H +L +R  EA
Sbjct: 121 AVFASR-PVTMATKTLSSGYLTTVVGPWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEA 180

Query: 181 DTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEEDH 240
           D L+++++ Q S N  GA++NVR   Q Y G+I+R+M+FN+ Y+G+GREDGGP  EEE+H
Sbjct: 181 DNLVKFLYNQCSANQNGAVVNVRIAAQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEH 240

Query: 241 NQALLTILRHVNAFSISDFMPCLKPFDI 269
             ALLT+L +  AF +SD++P L+ FDI
Sbjct: 241 VSALLTLLTYAYAFCVSDYLPWLRVFDI 257

BLAST of HG10007543 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-59
Identity = 120/260 (46.15%), Postives = 168/260 (64.62%), Query Frame = 0

Query: 13  YQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVR--RQAPLPPGPKPWPLVGCLPT 72
           + S  +  S TF++ V      + F   + + + HSV   ++  LPPGPKPWP+VG LP 
Sbjct: 7   FLSFCLEFSWTFLLVV------IFFFIIFKVTKSHSVNKSKKYKLPPGPKPWPIVGNLPE 66

Query: 73  MLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYDSVFASRSSIS 132
           ML N+ P   WIH +MK+ NTEIA IRL NT +IP+T P +  +FLK +D+ FASR  I 
Sbjct: 67  MLANR-PATIWIHKLMKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIM 126

Query: 133 NTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEADTLLRYIFTQ 192
           +T DI S G+LTT     G+QWKKM+R+L + +L+P     +L +R  EAD L+ YI+ +
Sbjct: 127 ST-DIASDGFLTTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNK 186

Query: 193 TSK--NGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEEDHNQALLTIL 252
             K  N G  ++N+R   QHY GN+ R+++FN RY+G+  EDGGP FEE +H  A  TIL
Sbjct: 187 CCKDVNDGPGLVNIRIAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTIL 246

Query: 253 RHVNAFSISDFMPCLKPFDI 269
           ++V AFSISDF+P L+  D+
Sbjct: 247 KYVYAFSISDFIPFLRRLDL 258

BLAST of HG10007543 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 9.6e-59
Identity = 117/246 (47.56%), Postives = 161/246 (65.45%), Query Frame = 0

Query: 25  IVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGPKPWPLVGCLPTMLTNKSPTHQWIHS 84
           ++F FM I  V  +   N  +K+       LPPGPKPWP+VG LP ML N+ P   WIH 
Sbjct: 22  VIFSFM-IFKVTKTHLVNKSKKYK------LPPGPKPWPIVGNLPEMLANR-PATIWIHK 81

Query: 85  IMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYDSVFASRSSISNTVDILSRGYLTTA 144
           +MK+ NTEIA IRL NT +IP+T P +  +FLK +D+ FASR  I +T DI S G++TT 
Sbjct: 82  LMKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIMST-DIASDGFITTV 141

Query: 145 FSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEADTLLRYIFTQTSK--NGGGAMINV 204
               G+QWKKM+R+L + +L+P     +L +R  EAD L+ YI+ +  K  N G  ++N+
Sbjct: 142 LVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGPGLVNI 201

Query: 205 RSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEEDHNQALLTILRHVNAFSISDFMPC 264
           R   QHY GN+ R+++FN RY+G+  EDGGP FEE +H  A  TIL++V AFSISDF+P 
Sbjct: 202 RIAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSISDFVPF 258

Query: 265 LKPFDI 269
           L+  D+
Sbjct: 262 LRRLDL 258

BLAST of HG10007543 vs. ExPASy Swiss-Prot
Match: A0A068Q7V0 (Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.9e-55
Identity = 100/230 (43.48%), Postives = 159/230 (69.13%), Query Frame = 0

Query: 42  NLKRKHSVRRQAPLPPGPK---PWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRL 101
           N K   ++++  PLPP PK   PWP+VG LP ML NK PT +WIH +M++ NT+IA IRL
Sbjct: 31  NDKNNKAIKQHYPLPPTPKGLRPWPIVGNLPEMLMNK-PTFRWIHKLMEESNTQIACIRL 90

Query: 102 GNTYIIPITSPELTLQFLKTYDSVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRI 161
            N ++ P++ P L+ + LK  D+ FA+R  +S +  ++++GY+TT     G+QWKKMR++
Sbjct: 91  ANVHVTPVSCPILSREILKKQDATFATR-PLSISTFLITKGYITTVMVPFGEQWKKMRKV 150

Query: 162 LASEILNPTTLHRMLAQRTAEADTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRML 221
           + SE+L+P     +  +R  EAD L+RY+F Q + + G  ++++R  TQHYC N+I+RM+
Sbjct: 151 ITSELLSPMRHKWLTDKRIEEADHLVRYVFNQCNNDEGSGIVDLRLATQHYCANVIKRMI 210

Query: 222 FNKRYYGEGREDGGPTFEEEDHNQALLTILRHVNAFSISDFMPCLKPFDI 269
           FN+RY+ E  +DGGP+ EE+++  A+  +LR++ AFS SD++ CL+  D+
Sbjct: 211 FNQRYFTEEMKDGGPSVEEQNYVNAVFDMLRYIYAFSASDYISCLRGLDL 258

BLAST of HG10007543 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.8e-55
Identity = 112/249 (44.98%), Postives = 159/249 (63.86%), Query Frame = 0

Query: 30  MAIISVMFSQCYNLKRKH-------SVRRQAPLPPGPKPWPLVGCLPTMLTNKSPTHQWI 89
           +A I  +      +KRK        S  R   LPPGPK WPL+G LP +L    P  +WI
Sbjct: 8   LAFIIGLLLLALTMKRKEKKKTMLISPTRNLSLPPGPKSWPLIGNLPEILGRNKPVFRWI 67

Query: 90  HSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYDSVFASRSSISNTVDILSRGYLT 149
           HS+MK+ NT+IA IRL NT++IP+TSP +  + LK  DSVFA+R     T +  SRGYLT
Sbjct: 68  HSLMKELNTDIACIRLANTHVIPVTSPRIAREILKKQDSVFATRPLTMGT-EYCSRGYLT 127

Query: 150 TAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEADTLLRYIFTQTSKNGGGA--MI 209
            A    G+QWKKMRR++AS + +  +   ML +RT EAD L+RYI  ++ KN G A  +I
Sbjct: 128 VAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQKRTEEADNLVRYINNRSVKNRGNAFVVI 187

Query: 210 NVRSITQHYCGNIIRRMLFNKRYYGEGREDG-GPTFEEEDHNQALLTILRHVNAFSISDF 269
           ++R   + Y GN+ R+M+F  R++G+G EDG GP  EE +H ++L T+L H+ AF++SD+
Sbjct: 188 DLRLAVRQYSGNVARKMMFGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTHLYAFALSDY 247

BLAST of HG10007543 vs. ExPASy TrEMBL
Match: A0A1S3BQ37 (isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491994 PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 2.6e-123
Identity = 222/269 (82.53%), Postives = 251/269 (93.31%), Query Frame = 0

Query: 1   MEAAF-LFISQLAYQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGP 60
           MEAAF +FI+QL +Q+S IAISK FIVF+FMA  SVMFS+CYN ++K+SVR+ A LPPGP
Sbjct: 1   MEAAFSIFITQLPHQNSPIAISKAFIVFLFMAFASVMFSRCYNRRKKYSVRK-ALLPPGP 60

Query: 61  KPWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTY 120
           KPWP+VGCLPTMLTNKSPT+QWIHS+MKQFNTEIA IRLGNTY+IP+TSPEL L+FL+T+
Sbjct: 61  KPWPIVGCLPTMLTNKSPTYQWIHSVMKQFNTEIACIRLGNTYVIPVTSPELALEFLRTH 120

Query: 121 DSVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAE 180
           DSVF+SRSSISNTVDILSRGYLTTAFSS+GDQWKKMRRILASEIL+PT LHRML QR AE
Sbjct: 121 DSVFSSRSSISNTVDILSRGYLTTAFSSMGDQWKKMRRILASEILSPTMLHRMLEQRIAE 180

Query: 181 ADTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEED 240
           ADTLL YIF+ TS+NGGGA+INVRSITQHYCGNIIR MLFN+RYYG+GREDGGPT EEE+
Sbjct: 181 ADTLLHYIFSITSENGGGAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTSEEEE 240

Query: 241 HNQALLTILRHVNAFSISDFMPCLKPFDI 269
           HNQALLTILRH+NAFSISDFMPCLKPFD+
Sbjct: 241 HNQALLTILRHINAFSISDFMPCLKPFDL 268

BLAST of HG10007543 vs. ExPASy TrEMBL
Match: A0A0A0LXR0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G620390 PE=4 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 5.4e-121
Identity = 219/269 (81.41%), Postives = 244/269 (90.71%), Query Frame = 0

Query: 1   MEAAFL-FISQLAYQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGP 60
           MEA FL F++QL +Q S IAISKTFI+F+FMA  SVMFS  YN ++K+SV R+  LPPGP
Sbjct: 1   MEAGFLIFLTQLTHQHSPIAISKTFIIFLFMAFASVMFSHYYNRRKKYSVVRKPRLPPGP 60

Query: 61  KPWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTY 120
           KPWP+VGCLPTMLTNKSPT  WIHS+MKQFNTEIA IRLG+TYIIP+TSPEL ++FLKT+
Sbjct: 61  KPWPIVGCLPTMLTNKSPTSHWIHSVMKQFNTEIACIRLGSTYIIPVTSPELAIEFLKTH 120

Query: 121 DSVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAE 180
           DSVF+SRSSISNTV ILSRGYLTTAFSS+GDQWKKMRRILASEILNPT LH+ML QRTAE
Sbjct: 121 DSVFSSRSSISNTVHILSRGYLTTAFSSMGDQWKKMRRILASEILNPTMLHQMLGQRTAE 180

Query: 181 ADTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEED 240
           ADTLLRYIF+ TS NGG A+INVRSITQHYCGNIIR MLFN+RYYG+GREDGGPT EEE+
Sbjct: 181 ADTLLRYIFSITSGNGGSAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTLEEEE 240

Query: 241 HNQALLTILRHVNAFSISDFMPCLKPFDI 269
           HNQALLTILRHVNAFSISDFMPCLKPFD+
Sbjct: 241 HNQALLTILRHVNAFSISDFMPCLKPFDL 269

BLAST of HG10007543 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.1e-109
Identity = 201/268 (75.00%), Postives = 232/268 (86.57%), Query Frame = 0

Query: 4   AFLFISQLAYQSSSIAISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGPKPWP 63
           A LFISQL YQ+S  AI+KTF +FVFMA IS +FSQCY  KRKHSV +   LPPGP PWP
Sbjct: 3   ALLFISQLTYQNSPTAITKTFTLFVFMAFISAIFSQCYR-KRKHSVLQ---LPPGPNPWP 62

Query: 64  LVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYDSVF 123
           LVGCLP ML  KSP ++WIH++MKQF+TEIA IRLGNTY++P+TSPEL ++FLK +DSVF
Sbjct: 63  LVGCLPAMLATKSPKYKWIHAVMKQFDTEIACIRLGNTYVMPVTSPELAVEFLKRHDSVF 122

Query: 124 ASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEADTL 183
           ASRS+ISNTV++LSRG LTTAFS +G QWKKMRRILASEI  P+TLH+ML  RT EADTL
Sbjct: 123 ASRSTISNTVNVLSRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEADTL 182

Query: 184 LRYIFTQTSK---NGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEEDH 243
           LRYIF+ T+    N  GA++NVRS+TQHYCGNIIRRMLFNKRYYG+GREDGGP+FEEE+H
Sbjct: 183 LRYIFSLTTTNTVNKDGAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPSFEEEEH 242

Query: 244 NQALLTILRHVNAFSISDFMPCLKPFDI 269
           NQALLTILRHVNAFSISDFMPCLKPFD+
Sbjct: 243 NQALLTILRHVNAFSISDFMPCLKPFDL 266

BLAST of HG10007543 vs. ExPASy TrEMBL
Match: A0A6J1D6G3 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017495 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.2e-107
Identity = 199/269 (73.98%), Postives = 233/269 (86.62%), Query Frame = 0

Query: 4   AFLFISQLAYQSSSI-AISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGPKPW 63
           AFL I QL+YQ+S   AI+KTF VF+FMA IS +FSQCY  KRKHSV +   LPPGPKPW
Sbjct: 3   AFLIIWQLSYQNSPTGAINKTFTVFLFMAFISAIFSQCYR-KRKHSVLQ---LPPGPKPW 62

Query: 64  PLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYDSV 123
           PLVGCLP+ML  KSP ++WIH++MKQF+TEIA IRLGNTY+IP+TSPEL ++FLK +DSV
Sbjct: 63  PLVGCLPSMLATKSPKYKWIHAVMKQFDTEIACIRLGNTYVIPVTSPELAVEFLKKHDSV 122

Query: 124 FASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEADT 183
           FASRS++SNTV++L+RG LTTAFS +G QWKKMRRILASEI  P+TLH+ML  RT EADT
Sbjct: 123 FASRSTVSNTVNVLTRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEADT 182

Query: 184 LLRYIF---TQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTFEEED 243
           LLRYIF   T  + N GGA++NV S+TQHYCGNIIRRMLFN+RYYG+GREDGGP+FEEE+
Sbjct: 183 LLRYIFSLTTTNTNNEGGAVVNVGSVTQHYCGNIIRRMLFNRRYYGKGREDGGPSFEEEE 242

Query: 244 HNQALLTILRHVNAFSISDFMPCLKPFDI 269
           HNQALLTILRHVNAFSISDFMPCLKPFD+
Sbjct: 243 HNQALLTILRHVNAFSISDFMPCLKPFDL 267

BLAST of HG10007543 vs. ExPASy TrEMBL
Match: A0A5D3DVF9 (Isoleucine N-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold225G00730 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.5e-107
Identity = 194/273 (71.06%), Postives = 227/273 (83.15%), Query Frame = 0

Query: 1   MEAAFLFISQLAYQSSSIA----ISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLP 60
           ME A +F  QL YQ+S +A    +  +F++F+FMA +S++F QCYN   KHS   Q PLP
Sbjct: 1   MEVASIFTGQLIYQNSPVAMISNLQPSFVMFLFMAFLSILFFQCYNRTEKHS-NLQPPLP 60

Query: 61  PGPKPWPLVGCLPTML-TNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQF 120
           PGPKPWPL+GCLP ML +N S TH+WIHSIMKQFN EIASIRL NTY+IP+ SPEL L+F
Sbjct: 61  PGPKPWPLLGCLPAMLSSNNSSTHEWIHSIMKQFNAEIASIRLRNTYVIPVASPELALEF 120

Query: 121 LKTYDSVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQ 180
           LKTYDSVF SRSS SN VD+L+ G ++T  S  G QW+KM+RIL SEILNP+TLHRML Q
Sbjct: 121 LKTYDSVFGSRSSFSNDVDMLTGGCVSTILSPTGPQWRKMKRILTSEILNPSTLHRMLGQ 180

Query: 181 RTAEADTLLRYIFTQTSKNGGGAMINVRSITQHYCGNIIRRMLFNKRYYGEGREDGGPTF 240
           RTAEAD LL YIF QT KNGGGA+INVRSITQHYCGNIIRR++FN+RYYG+GREDGGPTF
Sbjct: 181 RTAEADALLHYIFNQTCKNGGGAVINVRSITQHYCGNIIRRIVFNRRYYGKGREDGGPTF 240

Query: 241 EEEDHNQALLTILRHVNAFSISDFMPCLKPFDI 269
           EEE+HNQALLTI+RHVN+FSISDFMPCLKP D+
Sbjct: 241 EEEEHNQALLTIVRHVNSFSISDFMPCLKPLDL 272

BLAST of HG10007543 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 216.5 bits (550), Expect = 2.7e-56
Identity = 112/249 (44.98%), Postives = 159/249 (63.86%), Query Frame = 0

Query: 30  MAIISVMFSQCYNLKRKH-------SVRRQAPLPPGPKPWPLVGCLPTMLTNKSPTHQWI 89
           +A I  +      +KRK        S  R   LPPGPK WPL+G LP +L    P  +WI
Sbjct: 2   LAFIIGLLLLALTMKRKEKKKTMLISPTRNLSLPPGPKSWPLIGNLPEILGRNKPVFRWI 61

Query: 90  HSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYDSVFASRSSISNTVDILSRGYLT 149
           HS+MK+ NT+IA IRL NT++IP+TSP +  + LK  DSVFA+R     T +  SRGYLT
Sbjct: 62  HSLMKELNTDIACIRLANTHVIPVTSPRIAREILKKQDSVFATRPLTMGT-EYCSRGYLT 121

Query: 150 TAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEADTLLRYIFTQTSKNGGGA--MI 209
            A    G+QWKKMRR++AS + +  +   ML +RT EAD L+RYI  ++ KN G A  +I
Sbjct: 122 VAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQKRTEEADNLVRYINNRSVKNRGNAFVVI 181

Query: 210 NVRSITQHYCGNIIRRMLFNKRYYGEGREDG-GPTFEEEDHNQALLTILRHVNAFSISDF 269
           ++R   + Y GN+ R+M+F  R++G+G EDG GP  EE +H ++L T+L H+ AF++SD+
Sbjct: 182 DLRLAVRQYSGNVARKMMFGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTHLYAFALSDY 241

BLAST of HG10007543 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 211.1 bits (536), Expect = 1.1e-54
Identity = 106/223 (47.53%), Postives = 149/223 (66.82%), Query Frame = 0

Query: 51  RQAPLPPGPKPWPLVGCLPTMLTNKSPTHQWIHSIMKQFNTEIASIRLGNTYIIPITSPE 110
           R   LPPGPK WPLVG LP +L    P  +WIHS+M++ NT+IA IRL NT++IP+TSP 
Sbjct: 38  RNLSLPPGPKSWPLVGNLPEILGRNKPVFRWIHSLMEELNTDIACIRLANTHVIPVTSPR 97

Query: 111 LTLQFLKTYDSVFASRSSISNTVDILSRGYLTTAFSSVGDQWKKMRRILASEILNPTTLH 170
           +  + LK  DSVFA R     T +  SRGYLT A  S G+QWKKMRR++AS + +  +  
Sbjct: 98  IAREILKKQDSVFAIRPLTMGT-EYCSRGYLTIAVQSQGEQWKKMRRVVASHVTSKKSFK 157

Query: 171 RMLAQRTAEADTLLRYIFTQTSKNGGG----AMINVRSITQHYCGNIIRRMLFNKRYYGE 230
            ML +RT EAD L+RYI  +  KN G     A+I++R + + Y GN+ R+M+F  R++G+
Sbjct: 158 LMLEKRTEEADNLVRYINNRCVKNRGNGNGLAVIDLRFVVRQYSGNVARKMMFGIRHFGK 217

Query: 231 GRED-GGPTFEEEDHNQALLTILRHVNAFSISDFMPCLKPFDI 269
           G ED  GP  EE +H ++L T+L H+ AF++SD++P L+  D+
Sbjct: 218 GSEDESGPGSEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDL 259

BLAST of HG10007543 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 177.2 bits (448), Expect = 1.8e-44
Identity = 92/252 (36.51%), Postives = 150/252 (59.52%), Query Frame = 0

Query: 19  AISKTFIVFVFMAIISVMFSQCYN-LKRKHSVRRQAPLPPGPKPWPLVGCLPTMLTNKSP 78
           + +  +++    A+ ++ F    N +K     ++  PLPPGP  +P+VG +P ML N+ P
Sbjct: 21  SFTNMYLLTTLQALAALCFLMILNKIKSSSRNKKLHPLPPGPTGFPIVGMIPAMLKNR-P 80

Query: 79  THQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYDSVFASRSSISNTVDILS 138
             +W+HS+MK+ NTEIA +RLGNT++IP+T P++  +  K  D++FASR  ++    ILS
Sbjct: 81  VFRWLHSLMKELNTEIACVRLGNTHVIPVTCPKIAREIFKQQDALFASR-PLTYAQKILS 140

Query: 139 RGYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQRTAEADTLLRYIFTQTSKNGGG 198
            GY T   +  G+Q+KKMR+++ +EI+ P     +   R  E D L  +++     +   
Sbjct: 141 NGYKTCVITPFGEQFKKMRKVIMTEIVCPARHRWLHDNRAEETDHLTAWLYNMVKNS--- 200

Query: 199 AMINVRSITQHYCGNIIRRMLFNKRYYGEGRE-DGGPTFEEEDHNQALLTILRHVNAFSI 258
             +++R +T+HYCGN I+R++F  R + E  E DGGPT E+ +H  A+   L    AF I
Sbjct: 201 EPVDLRFVTRHYCGNAIKRLMFGTRTFSEKTEADGGPTLEDIEHMDAMFEGLGFTFAFCI 260

Query: 259 SDFMPCLKPFDI 269
           SD++P L   D+
Sbjct: 261 SDYLPMLTGLDL 267

BLAST of HG10007543 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 176.8 bits (447), Expect = 2.4e-44
Identity = 92/252 (36.51%), Postives = 151/252 (59.92%), Query Frame = 0

Query: 19  AISKTFIVFVFMAIISVMFSQCYNLKRKHSVRRQAPLPPGPKPWPLVGCLPTMLTNKSPT 78
           + S  +++    A +++              +++  LPPGP  WP++G +PTML ++ P 
Sbjct: 20  SFSTLYLLSTLQAFVAITLVMLLKKLMTDPNKKKPYLPPGPTGWPIIGMIPTMLKSR-PV 79

Query: 79  HQWIHSIMKQFNTEIASIRLGNTYIIPITSPELTLQFLKTYDSVFASRSSISNTVDILSR 138
            +W+HSIMKQ NTEIA ++LGNT++I +T P++  + LK  D++FASR  ++    ILS 
Sbjct: 80  FRWLHSIMKQLNTEIACVKLGNTHVITVTCPKIAREILKQQDALFASR-PLTYAQKILSN 139

Query: 139 GYLTTAFSSVGDQWKKMRRILASEILNPTTLHRMLAQ-RTAEADTLLRYIFTQTSKNGGG 198
           GY T   +  GDQ+KKMR+++ +E++ P   HR L Q R+ E D L  +++     +G  
Sbjct: 140 GYKTCVITPFGDQFKKMRKVVMTELVCPAR-HRWLHQKRSEENDHLTAWVYNMVKNSGS- 199

Query: 199 AMINVRSITQHYCGNIIRRMLFNKRYYGEG-REDGGPTFEEEDHNQALLTILRHVNAFSI 258
             ++ R +T+HYCGN I++++F  R + +    DGGPT E+ +H +A+   L    AF I
Sbjct: 200 --VDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPDGGPTVEDVEHMEAMFEALGFTFAFCI 259

Query: 259 SDFMPCLKPFDI 269
           SD++P L   D+
Sbjct: 260 SDYLPMLTGLDL 265

BLAST of HG10007543 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 150.6 bits (379), Expect = 1.8e-36
Identity = 73/239 (30.54%), Postives = 137/239 (57.32%), Query Frame = 0

Query: 30  MAIISVMFSQCYNLKRKHSVRRQAPLPPGPKPWPLVGCLPTMLTNKSPTHQWIHSIMKQF 89
           + +IS+        +    ++ +  LPPGP+ WP+VG +  M+ N+ P H WIH +M++ 
Sbjct: 12  LVLISITLVLALARRFSRFMKPKGQLPPGPRGWPIVGNMLQMIINR-PAHLWIHRVMEEL 71

Query: 90  NTEIASIRLGNTYIIPITSPELTLQFLKTYDSVFASRSSISNTVDILSRGYLTTAFSSVG 149
            T+IA  R    ++I +TS ++  + L+  D V A RS  S    ++S GY   +FSS G
Sbjct: 72  QTDIACYRFARFHVITVTSSKIAREVLREKDEVLADRSE-SYASHLISHGYKNISFSSYG 131

Query: 150 DQWKKMRRILASEILNPTTLHRMLAQRTAEADTLLRYIFTQTSKNGGGAMINVRSITQHY 209
           + WK +++++ +++++PTTL + L  R  EAD ++ Y++           INVR     Y
Sbjct: 132 ENWKLVKKVMTTKLMSPTTLSKTLGYRNIEADNIVTYVYNLCQLGSVRKPINVRDTILTY 191

Query: 210 CGNIIRRMLFNKRYYGEGREDGGPTFEEEDHNQALLTILRHVNAFSISDFMPCLKPFDI 269
           C  ++ RM+F +R++ E  E+GG   +E++H  A+   L    +F++++++P L+ +++
Sbjct: 192 CHAVMMRMMFGQRHFDEVVENGGLGPKEKEHMDAIYLALDCFFSFNLTNYIPFLRGWNV 248

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878794.11.3e-13288.81tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida][more]
XP_038880015.12.7e-12786.19tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida][more]
XP_008450367.25.3e-12382.53PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis melo][more]
XP_004151661.21.1e-12081.41isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8653524.1 hypothetical protei... [more]
XP_038880438.16.3e-11678.15tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A068Q6056.2e-6648.51Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV... [more]
Q6J5401.1e-5946.15Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1[more]
Q6J5419.6e-5947.56Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1[more]
A0A068Q7V02.9e-5543.48Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1... [more]
Q9FLC83.8e-5544.98Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A1S3BQ372.6e-12382.53isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491994 PE=3 S... [more]
A0A0A0LXR05.4e-12181.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G620390 PE=4 SV=1[more]
A0A6J1D6G92.1e-10975.00isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498... [more]
A0A6J1D6G31.2e-10773.98isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017495... [more]
A0A5D3DVF91.5e-10771.06Isoleucine N-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT5G05260.12.7e-5644.98cytochrome p450 79a2 [more]
AT5G35917.11.1e-5447.53cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene [more]
AT2G22330.11.8e-4436.51cytochrome P450, family 79, subfamily B, polypeptide 3 [more]
AT4G39950.12.4e-4436.51cytochrome P450, family 79, subfamily B, polypeptide 2 [more]
AT1G58260.11.8e-3630.54cytochrome p450 79c2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PFAMPF00067p450coord: 56..265
e-value: 7.9E-16
score: 57.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 47..271
e-value: 3.5E-30
score: 106.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 56..268
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 29..268
NoneNo IPR availablePANTHERPTHR24298:SF675PHENYLALANINE N-MONOOXYGENASEcoord: 29..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10007543.1HG10007543.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen