Homology
BLAST of HG10007403 vs. NCBI nr
Match:
XP_038880052.1 (L-ascorbate oxidase-like [Benincasa hispida])
HSP 1 Score: 976.5 bits (2523), Expect = 9.7e-281
Identity = 463/590 (78.47%), Postives = 499/590 (84.58%), Query Frame = 0
Query: 1 MAKLAKQPLFPMPFLVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVM 60
MAK A QPLFP+PFLVIF + S+ISGFGITLSEAV+ TR++KWE+EYMFWSPDC EN+VM
Sbjct: 1 MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM 60
Query: 61 GINGQFPRPTIRANVGDTIVVEMINKLSTEGVA--------------------------- 120
GINGQFP PTIRANVGDTI+VE++NKL TEGV
Sbjct: 61 GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP 120
Query: 121 -------------GTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWW 180
GTYFYHGHLGMQ +A L GSLI+D EE KKEPFHYDE+INLLLSDWW
Sbjct: 121 GETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWW 180
Query: 181 HKTVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPFI 240
HK+ KQEVGL+S+PMRWI EPE+ILINGRGQI+CSIAAKY K +EQCKLKG E+CAPFI
Sbjct: 181 HKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFI 240
Query: 241 LKVKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVL 300
L+VKP KTYRIRIASTTSLSALN AIGNHQLLVVEADGNY++PF+TSDIDIYSGESYSVL
Sbjct: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 300
Query: 301 ITTNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKN 360
ITTNQ P +NYWVS+GVRGRLPKTPPALTLLNYLPNSV KLP+SPPPETP W+DY RSKN
Sbjct: 301 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 360
Query: 361 FTNRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGL 420
FT RILA MGSP PP+K NRRIFLLNTQN +NGGF KWAINNVSLSLP TPYL AI+IGL
Sbjct: 361 FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL 420
Query: 421 NNTFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEI 480
NNTF+ NPPPETF EDYDI NPPPN NT T NGVY F LGE VDVILQNANTL +K SEI
Sbjct: 421 NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI 480
Query: 481 HPWHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
HPWHLHGHDFWVLGYGEGKFS VDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
Query: 541 PFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
FHCHIEPHLHMGMGV+FAEGVHKVG IPTKALACGGTAK LINNPT+KP
Sbjct: 541 AFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 590
BLAST of HG10007403 vs. NCBI nr
Match:
NP_001284440.1 (L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucumis melo])
HSP 1 Score: 954.5 bits (2466), Expect = 3.9e-274
Identity = 455/588 (77.38%), Postives = 496/588 (84.35%), Query Frame = 0
Query: 3 KLAKQPLFPMPFLVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGI 62
K+A QPLFPMPF IFF+ SI GFGITLS+A S+TRH+KWE+EYMF SPDC EN+VMGI
Sbjct: 4 KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPRPTIRANVGDTIVVEMINKLSTEGVA----------------------------- 122
N QFP PTIRANVGDT+VVE+INKLSTE V
Sbjct: 64 NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 -----------GTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHK 182
GTYFYHGHLGMQ AA L GSLIVDLEE KKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 TVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPFILK 242
+V KQEVGL+S+PMRWI EP+SILINGRGQ NCSI AKY K +EQCKL GSEECAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LLVVEADGNY+QPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFT 362
T+Q P +NYWVS+GVRGR PKTPP LTLLNYLPNS +KLPISPPPETPKW+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 NRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNN 422
RILA MGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL+LP TPYL AI+IG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHP 482
TFD NPPPET+PE+YDI+ PPPN NT TGNGVY+F+LGEIVDV+LQNANTL +K SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYGEGKFS VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
HCHIEPHLHMGMGVVFAEGV KVGRIPT A+ACGGTAKALI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of HG10007403 vs. NCBI nr
Match:
TYK09892.1 (L-ascorbate oxidase-like [Cucumis melo var. makuwa])
HSP 1 Score: 950.3 bits (2455), Expect = 7.4e-273
Identity = 455/588 (77.38%), Postives = 494/588 (84.01%), Query Frame = 0
Query: 3 KLAKQPLFPMPFLVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGI 62
K A QPLFPMPF IFF+ SI GFGITLS+A S+TRH+KWE+EYMF SPDC EN+VMGI
Sbjct: 4 KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPRPTIRANVGDTIVVEMINKLSTEGVA----------------------------- 122
N QFP PTIRANVGDTIVVE+INKLSTE V
Sbjct: 64 NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 -----------GTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHK 182
GTYFYHGHLGMQ AA L GSLIVDLEE KKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 TVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPFILK 242
+V KQEVGL+S+PMRWI EP+SILINGRGQ NCSI AKY K +EQCKL GSE CAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LLVVEADGNY+QPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFT 362
T+Q P +NYWVS+GVRGR PKTPP LTLLNYLPNS +KLPISPPPETPKW+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 NRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNN 422
RILA MGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL+LP TPYL AI+IG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHP 482
TFD NPPPET+PE+YDI+ PPPN NT TGNGVY+F+LGEIVDV+LQNANTL +K SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYGEGKFS VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
HCHIEPHLHMGMGVVFAEGV KVGRIPT A+ACGGTAKALI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of HG10007403 vs. NCBI nr
Match:
XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])
HSP 1 Score: 925.6 bits (2391), Expect = 2.0e-265
Identity = 451/592 (76.18%), Postives = 490/592 (82.77%), Query Frame = 0
Query: 1 MAKLAKQPLFPMPFLVIFFLLSIISGFGITLSEA-VSKTRHYKWEIEYMFWSPDCAENIV 60
MAK+A + LFP PFL IFF+LSII GFGITLSEA K RHYKWE+EYMFWSPDC ENIV
Sbjct: 1 MAKVADKSLFPKPFL-IFFILSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPRPTIRANVGDTIVVEMINKLSTEGV--------------------------- 120
MGI+GQFP PTIRANVGDT+VVE+ NKL TEGV
Sbjct: 61 MGIDGQFPGPTIRANVGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAIN 120
Query: 121 -------------AGTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDW 180
AGTYFYHGHLGMQ +A L GSLIVD EE K EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDW 180
Query: 181 WHKTVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPF 240
WH++V KQEVGL+S P+RWI EP++ILINGRGQ +CSIAAKY ++QC+LKG E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPIRWIGEPQTILINGRGQFDCSIAAKYNNNLKQCELKGGEQCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSV 300
IL V PNKTYRIRIASTT+L++LNFAIGNH+LLVVEADGNYIQPFLTSDIDIYSGESYSV
Sbjct: 241 ILHVLPNKTYRIRIASTTALASLNFAIGNHRLLVVEADGNYIQPFLTSDIDIYSGESYSV 300
Query: 301 LITTNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSK 360
LITT+Q ENYWVSIGVR RLPKTPP LT+LNYLPNSV+KLPISPPPETP W+D+DRSK
Sbjct: 301 LITTDQPSSENYWVSIGVRARLPKTPPGLTILNYLPNSVSKLPISPPPETPDWEDFDRSK 360
Query: 361 NFTNRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIG 420
NFT RI A MGSPKPP++ NRRIFLLNTQN +N GFVKWAINNVSL+LPPTPYLAA++
Sbjct: 361 NFTFRIFAAMGSPKPPMRYNRRIFLLNTQNRMN-GFVKWAINNVSLALPPTPYLAAMKFK 420
Query: 421 LNNTFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSE 480
LN FD NPPPETFP DYDINNPP N NT TGNGVY+F +GEIVDVILQNAN L + SE
Sbjct: 421 LNTAFDQNPPPETFPIDYDINNPPQNANTITGNGVYRFNMGEIVDVILQNANMLNDNTSE 480
Query: 481 IHPWHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYGEGKFS VDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALI-NNPTKKP 551
W FHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGG AKALI NNP + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGAAKALINNNPPQDP 590
BLAST of HG10007403 vs. NCBI nr
Match:
XP_038880028.1 (L-ascorbate oxidase-like [Benincasa hispida])
HSP 1 Score: 917.1 bits (2369), Expect = 7.0e-263
Identity = 441/590 (74.75%), Postives = 479/590 (81.19%), Query Frame = 0
Query: 1 MAKLAKQPLFPMPFLVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVM 60
MA++A +PLFPMPF VIF +LSIISGFGIT S+A S+ R++ WEI+YMFWSPDC ENI+M
Sbjct: 1 MAEVANRPLFPMPFPVIFLVLSIISGFGITPSQAASQIRNHTWEIDYMFWSPDCVENIIM 60
Query: 61 GINGQFPRPTIRANVGDTIVVEMINKLSTEGV---------------------------- 120
GINGQFP PTIRANV DTI+V + NKLSTEGV
Sbjct: 61 GINGQFPGPTIRANVSDTIIVHVNNKLSTEGVVIHWHGILQRGTPWADGTASISQCAINP 120
Query: 121 ------------AGTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWW 180
AGTYFYHGHLGMQ +A L GSLIVDLEE KKEPF YDE+INLLLSDWW
Sbjct: 121 GENFTYQFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDLEEGKKEPFDYDEEINLLLSDWW 180
Query: 181 HKTVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPFI 240
HK+V KQEVGLNSNP RWI EPESILINGRGQ NCSIAAKY +EQC+LK +EECAPFI
Sbjct: 181 HKSVHKQEVGLNSNPFRWIGEPESILINGRGQFNCSIAAKYNNNLEQCELKENEECAPFI 240
Query: 241 LKVKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVL 300
L+VKP KTYRIRIASTTSLSALN AIGNH LLVVEADGNY+ PF+T DIDIYSGESYSVL
Sbjct: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHSLLVVEADGNYVAPFVTYDIDIYSGESYSVL 300
Query: 301 ITTNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKN 360
ITTNQ P +NYWVS+GVRGRLP+T PALTLLNYLPNSV KLPISPPPETPKWDD+ RSK
Sbjct: 301 ITTNQDPAQNYWVSVGVRGRLPETAPALTLLNYLPNSVDKLPISPPPETPKWDDFARSKR 360
Query: 361 FTNRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGL 420
FT RILAT PKPPV NRRIFLLNTQN + GG +KWAINNVSL LP TPYL AI+IGL
Sbjct: 361 FTGRILATEDIPKPPVNFNRRIFLLNTQNLMKGGSIKWAINNVSLVLPSTPYLGAIKIGL 420
Query: 421 NNTFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEI 480
TFD NPPPETFPEDYDIN PPPN NTTTGNGVY+F+LGE+VDVILQNAN L+ S+I
Sbjct: 421 KTTFDQNPPPETFPEDYDINKPPPNPNTTTGNGVYKFELGEVVDVILQNANMLKENTSDI 480
Query: 481 HPWHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
HPWHLHGHDFWVLGYGEGKFS DVDE LNL+NPPL+NTVVIFP GWTAIRFVADNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSADVDENNLNLENPPLKNTVVIFPRGWTAIRFVADNPGVW 540
Query: 541 PFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
FHCHIEPHLHMGMGV+FAE VH+VG IPT ALACGG A+ LI P KP
Sbjct: 541 AFHCHIEPHLHMGMGVIFAEEVHRVGTIPTHALACGGIARTLIYRPATKP 590
BLAST of HG10007403 vs. ExPASy Swiss-Prot
Match:
P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)
HSP 1 Score: 892.5 bits (2305), Expect = 2.4e-258
Identity = 431/591 (72.93%), Postives = 478/591 (80.88%), Query Frame = 0
Query: 1 MAKLAKQPLFPMPFLVIFFLLSIISGFGITLSEA-VSKTRHYKWEIEYMFWSPDCAENIV 60
MAK+A +P FP PFL F +LSII GFGITLSEA K +HYKW++EYMFWSPDC ENIV
Sbjct: 1 MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPRPTIRANVGDTIVVEMINKLSTEGV--------------------------- 120
MGING+FP PTIRAN GD +VVE+ NKL TEGV
Sbjct: 61 MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 -------------AGTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDW 180
AGTYFYHGHLGMQ +A L GSLIVD E + EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180
Query: 181 WHKTVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPF 240
WH++V KQEVGL+S PMRWI EP+SILING+GQ +CSIAAKY +G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSV 300
IL V+P KTYRIRIASTT+L++LNFAIGNH+LLVVEADGNY+QPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300
Query: 301 LITTNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSK 360
LITT+Q PLENYWVSIGVR RLPKTPP LTLLNYLPNS +KLPISPPPETP W+D+DRSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360
Query: 361 NFTNRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIG 420
NFT RI A MGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSL+LPPTPYLAA+++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420
Query: 421 LNNTFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSE 480
LN F+ NPPPETFP +YDINNPPPN TTTGNGVY+F +GE VDVILQNAN L SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480
Query: 481 IHPWHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYGEGKF DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
W FHCHIEPHLHMGMGVVFAEGVH VG IP KALACG T AL+ N + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGST--ALVKNYPRLP 587
BLAST of HG10007403 vs. ExPASy Swiss-Prot
Match:
P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)
HSP 1 Score: 860.9 bits (2223), Expect = 7.8e-249
Identity = 413/574 (71.95%), Postives = 457/574 (79.62%), Query Frame = 0
Query: 13 PFLVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGINGQFPRPTIR 72
P +I F +I FGI S S+ RHYKWE+EYMFW+PDC ENIVMGINGQFP PTIR
Sbjct: 10 PNFLILFFFGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIR 69
Query: 73 ANVGDTIVVEMINKLSTEGVA--------------------------------------- 132
AN GDT+VVE+INKL TEGV
Sbjct: 70 ANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDN 129
Query: 133 -GTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHKTVQKQEVGLN 192
GT+FYHGHLGMQ +A L GSLIVD + KKEPFHYD +INLLLSDWWH+++ KQEVGL+
Sbjct: 130 PGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLS 189
Query: 193 SNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPFILKVKPNKTYRIR 252
S P+RWI EP++IL+NGRGQ +CSIAAKY +E CKLKGSE CAP+I V P KTYRIR
Sbjct: 190 SKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIR 249
Query: 253 IASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTNQTPLENYW 312
IASTT+L+ALNFAIGNH LLVVEADGNY+QPF TSDIDIYSGESYSVLITT+Q P ENYW
Sbjct: 250 IASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYW 309
Query: 313 VSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFTNRILATMGSP 372
VS+G RGR P TPP LTLLNYLPNSV+KLP SPPPETP WDD+DRSKNFT RI A MGSP
Sbjct: 310 VSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSP 369
Query: 373 KPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNNTFDHNPPPET 432
KPPVKSNRRIFLLNTQN IN G+VKWAIN+VSL+LPPTPYL A++ L + FD NPPPE
Sbjct: 370 KPPVKSNRRIFLLNTQNVIN-GYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEV 429
Query: 433 FPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHPWHLHGHDFWV 492
FPEDYDI+ PP N T GNGVYQFK+GEIVDVILQNAN ++ SEIHPWHLHGHDFWV
Sbjct: 430 FPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWV 489
Query: 493 LGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHM 547
LGYG+GKF+ + +E LNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHM
Sbjct: 490 LGYGDGKFTAE-EESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHM 549
BLAST of HG10007403 vs. ExPASy Swiss-Prot
Match:
P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)
HSP 1 Score: 844.7 bits (2181), Expect = 5.8e-244
Identity = 399/551 (72.41%), Postives = 444/551 (80.58%), Query Frame = 0
Query: 36 SKTRHYKWEIEYMFWSPDCAENIVMGINGQFPRPTIRANVGDTIVVEMINKLSTEGVA-- 95
S+ RHYKWE+EYMFW+P+C ENIVMGINGQFP PTIRAN GD++VVE+ NKL TEGV
Sbjct: 1 SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60
Query: 96 --------------------------------------GTYFYHGHLGMQIAARLNGSLI 155
GT+FYHGHLGMQ +A L GSLI
Sbjct: 61 WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120
Query: 156 VDLEEEKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNSNPMRWINEPESILINGRGQINC 215
VD + KKEPFHYD +INLLLSDWWH+++ KQEVGL+S P+RWI EP++IL+NGRGQ +C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180
Query: 216 SIAAKYKKGVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLVVE 275
SIAAKY +E CKLKGSE CAP+I V P KTYRIRIASTT+L+ALNFAIGNHQLLVVE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240
Query: 276 ADGNYIQPFLTSDIDIYSGESYSVLITTNQTPLENYWVSIGVRGRLPKTPPALTLLNYLP 335
ADGNY+QPF TSDIDIYSGESYSVLITT+Q P ENYWVS+G R R P TPP LTLLNYLP
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300
Query: 336 NSVTKLPISPPPETPKWDDYDRSKNFTNRILATMGSPKPPVKSNRRIFLLNTQNHINGGF 395
NSV+KLP SPPP+TP WDD+DRSKNFT RI A MGSPKPPVK NRRIFLLNTQN IN G+
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVIN-GY 360
Query: 396 VKWAINNVSLSLPPTPYLAAIRIGLNNTFDHNPPPETFPEDYDINNPPPNMNTTTGNGVY 455
VKWAIN+VSL+LPPTPYL A++ L + FD NPPPE FPEDYDI+ PP N T GNGVY
Sbjct: 361 VKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVY 420
Query: 456 QFKLGEIVDVILQNANTLENKRSEIHPWHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPP 515
QFK+GE+VDVILQNAN ++ SE HPWHLHGHDFWVLGYG+GKFS + +E LNLKNPP
Sbjct: 421 QFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAE-EESSLNLKNPP 480
Query: 516 LRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALAC 547
LRNTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHMGMGVVFAEGV KVGRIPTKALAC
Sbjct: 481 LRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALAC 540
BLAST of HG10007403 vs. ExPASy Swiss-Prot
Match:
Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)
HSP 1 Score: 724.5 bits (1869), Expect = 8.7e-208
Identity = 346/573 (60.38%), Postives = 417/573 (72.77%), Query Frame = 0
Query: 14 FLVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGINGQFPRPTIRA 73
FL F L I+ + S +KTRH+KW++EY+ WSPD E++VMGINGQFP PTIRA
Sbjct: 6 FLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRA 65
Query: 74 NVGDTIVVEMINKLSTEGV----------------------------------------A 133
GDT+ V + NKL TEGV A
Sbjct: 66 KAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKA 125
Query: 134 GTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNS 193
GTYFYHGH GMQ +A L GSLIV++ E +KEPFHYD + NLLLSDWWHK +QEV L+S
Sbjct: 126 GTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS 185
Query: 194 NPMRWINEPESILINGRGQINCSIAAKYKK-GVEQCKLKGSEECAPFILKVKPNKTYRIR 253
NP+RWI EP+++L+NGRGQ NCS+AA++ K + QCKL+G E+ AP IL+V+PNK YR+R
Sbjct: 186 NPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 245
Query: 254 IASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTNQTPLENYW 313
+ASTT+L +L+ AIG H+++VVEADGNY+QPF D+DIYSGESYSVL T+Q P +NYW
Sbjct: 246 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 305
Query: 314 VSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFTNRILATMGSP 373
+SI VRGR PKTP LTLLNYLPNS +K P PPP P W+DY+ SK+F+N+I A MGSP
Sbjct: 306 ISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSP 365
Query: 374 KPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNNTFDHNPPPET 433
KPP +++RRI LLNTQN I+ G+ KWAINNVSL LP YL +IR G+ N FD PPP+
Sbjct: 366 KPPPQNHRRIILLNTQNKID-GYTKWAINNVSLVLPTQLYLGSIRYGI-NAFDTKPPPDN 425
Query: 434 FPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHPWHLHGHDFWV 493
FP+DYD+ PN N+T GNGVY K +D+ILQNAN L SEIHPWHLHGHDFWV
Sbjct: 426 FPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWV 485
Query: 494 LGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHM 546
LGYGEGKFS + D KK NLKNPPLRNT VIFP+GWTA+RFV DNPGVW FHCHIEPHLHM
Sbjct: 486 LGYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHM 545
BLAST of HG10007403 vs. ExPASy Swiss-Prot
Match:
M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)
HSP 1 Score: 677.9 bits (1748), Expect = 9.3e-194
Identity = 322/562 (57.30%), Postives = 393/562 (69.93%), Query Frame = 0
Query: 24 ISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGINGQFPRPTIRANVGDTIVVEM 83
I I A + R Y WE+EY F PDC E +VM +NGQFP PTI A GDTIVV +
Sbjct: 6 IVAVAILAHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHL 65
Query: 84 INKLSTEGVA----------------------------------------GTYFYHGHLG 143
NKL+TEG+ GT+FYHGH G
Sbjct: 66 TNKLATEGLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYG 125
Query: 144 MQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNSNPMRWINEPE 203
MQ +A L GSLI+D+ + KKEP YD + NLLLSDWWH+ V QE+GL+S PMRWI E +
Sbjct: 126 MQRSAGLYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQ 185
Query: 204 SILINGRGQINCSIAAKYKK-GVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSAL 263
SILINGRGQ NCS+AA++ + C K ++CAP L V+PNKTYRIR+AS+T+L++L
Sbjct: 186 SILINGRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASL 245
Query: 264 NFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTNQTPLENYWVSIGVRGRLP 323
NFA+ H+L+VVEADGNYI PF T DIDIYSGE+YSVL+TT+Q P +NY+++ GVRGR P
Sbjct: 246 NFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKP 305
Query: 324 KTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFTNRILATMGSPKPPVKSNRRI 383
TPPALT+LNY+ ++LP SPPPETP+W+D+DRSKNF+ +I A MGSP PP + R+
Sbjct: 306 NTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFDERL 365
Query: 384 FLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNNTFDHNPPPETFPEDYDINNP 443
LLNTQN I GF KWAINNVSL++P TPYL +++ L F+ + PP+ +P DYDI P
Sbjct: 366 ILLNTQNLIE-GFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTP 425
Query: 444 PPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHPWHLHGHDFWVLGYGEGKFSG 503
P N N GN F VDVILQNAN L SEIHPWHLHGHDFWVLGYGEGKF
Sbjct: 426 PRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKP 485
Query: 504 DVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGV 545
VDEK NLKNPPLRNTV ++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGVVFAEG+
Sbjct: 486 GVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGL 545
BLAST of HG10007403 vs. ExPASy TrEMBL
Match:
Q9M5B5 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1)
HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 455/588 (77.38%), Postives = 496/588 (84.35%), Query Frame = 0
Query: 3 KLAKQPLFPMPFLVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGI 62
K+A QPLFPMPF IFF+ SI GFGITLS+A S+TRH+KWE+EYMF SPDC EN+VMGI
Sbjct: 4 KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPRPTIRANVGDTIVVEMINKLSTEGVA----------------------------- 122
N QFP PTIRANVGDT+VVE+INKLSTE V
Sbjct: 64 NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 -----------GTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHK 182
GTYFYHGHLGMQ AA L GSLIVDLEE KKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 TVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPFILK 242
+V KQEVGL+S+PMRWI EP+SILINGRGQ NCSI AKY K +EQCKL GSEECAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LLVVEADGNY+QPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFT 362
T+Q P +NYWVS+GVRGR PKTPP LTLLNYLPNS +KLPISPPPETPKW+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 NRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNN 422
RILA MGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL+LP TPYL AI+IG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHP 482
TFD NPPPET+PE+YDI+ PPPN NT TGNGVY+F+LGEIVDV+LQNANTL +K SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYGEGKFS VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
HCHIEPHLHMGMGVVFAEGV KVGRIPT A+ACGGTAKALI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of HG10007403 vs. ExPASy TrEMBL
Match:
A0A5D3CF11 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00020 PE=3 SV=1)
HSP 1 Score: 950.3 bits (2455), Expect = 3.6e-273
Identity = 455/588 (77.38%), Postives = 494/588 (84.01%), Query Frame = 0
Query: 3 KLAKQPLFPMPFLVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGI 62
K A QPLFPMPF IFF+ SI GFGITLS+A S+TRH+KWE+EYMF SPDC EN+VMGI
Sbjct: 4 KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPRPTIRANVGDTIVVEMINKLSTEGVA----------------------------- 122
N QFP PTIRANVGDTIVVE+INKLSTE V
Sbjct: 64 NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 -----------GTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHK 182
GTYFYHGHLGMQ AA L GSLIVDLEE KKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 TVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPFILK 242
+V KQEVGL+S+PMRWI EP+SILINGRGQ NCSI AKY K +EQCKL GSE CAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LLVVEADGNY+QPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFT 362
T+Q P +NYWVS+GVRGR PKTPP LTLLNYLPNS +KLPISPPPETPKW+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 NRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNN 422
RILA MGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL+LP TPYL AI+IG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHP 482
TFD NPPPET+PE+YDI+ PPPN NT TGNGVY+F+LGEIVDV+LQNANTL +K SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYGEGKFS VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
HCHIEPHLHMGMGVVFAEGV KVGRIPT A+ACGGTAKALI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of HG10007403 vs. ExPASy TrEMBL
Match:
A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)
HSP 1 Score: 893.6 bits (2308), Expect = 4.0e-256
Identity = 433/591 (73.27%), Postives = 476/591 (80.54%), Query Frame = 0
Query: 1 MAKLAKQPLFPMPFLVIFFLLSIISGFGITLSEA-VSKTRHYKWEIEYMFWSPDCAENIV 60
MAK+A +P FP PFLV FF+LSII GFGITLSEA K RHYKWE+EYMFWSPDC ENIV
Sbjct: 1 MAKVADKPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPRPTIRANVGDTIVVEMINKLSTEGV--------------------------- 120
MGINGQFP PTIRAN GD +VVE+ NKL TEGV
Sbjct: 61 MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 -------------AGTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDW 180
AGTYFYHGHLGMQ +A L GSLIVD +E EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180
Query: 181 WHKTVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPF 240
WH++V KQEVGL+S PMRWI EP+SILINGRGQ +CSIAAKYK G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSV 300
IL V P KTYRIRIASTT+L++LNFAIGNH+LLVVEADGNY+QPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300
Query: 301 LITTNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSK 360
LITT+Q P ENYWVSIGVR RLPKTPP LT+LNYLPNSV+KLPISPPPETP W+D+DRSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360
Query: 361 NFTNRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIG 420
NFT RI A MGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSL+LPPTPYLAA++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420
Query: 421 LNNTFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSE 480
L+ F+ NPPPETFP DYDINNPP N T TGNGVY+F +GE VDVILQNAN L SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480
Query: 481 IHPWHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYGEGKFS D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
W FHCHIEPHLHMGMGV+FAEGVHKVG IP A+ACG T A+I N +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGST--AMIKNYPRKP 587
BLAST of HG10007403 vs. ExPASy TrEMBL
Match:
A0A0A0LXB9 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)
HSP 1 Score: 892.5 bits (2305), Expect = 8.9e-256
Identity = 431/591 (72.93%), Postives = 478/591 (80.88%), Query Frame = 0
Query: 1 MAKLAKQPLFPMPFLVIFFLLSIISGFGITLSEA-VSKTRHYKWEIEYMFWSPDCAENIV 60
MAK+A +P FP PFL F +LSII GFGITLSEA K +HYKW++EYMFWSPDC ENIV
Sbjct: 1 MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPRPTIRANVGDTIVVEMINKLSTEGV--------------------------- 120
MGING+FP PTIRAN GD +VVE+ NKL TEGV
Sbjct: 61 MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 -------------AGTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDW 180
AGTYFYHGHLGMQ +A L GSLIVD E + EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180
Query: 181 WHKTVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPF 240
WH++V KQEVGL+S PMRWI EP+SILING+GQ +CSIAAKY +G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSV 300
IL V+P KTYRIRIASTT+L++LNFAIGNH+LLVVEADGNY+QPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300
Query: 301 LITTNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSK 360
LITT+Q PLENYWVSIGVR RLPKTPP LTLLNYLPNS +KLPISPPPETP W+D+DRSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360
Query: 361 NFTNRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIG 420
NFT RI A MGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSL+LPPTPYLAA+++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420
Query: 421 LNNTFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSE 480
LN F+ NPPPETFP +YDINNPPPN TTTGNGVY+F +GE VDVILQNAN L SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480
Query: 481 IHPWHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYGEGKF DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
W FHCHIEPHLHMGMGVVFAEGVH VG IP KALACG T AL+ N + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGST--ALVKNYPRLP 587
BLAST of HG10007403 vs. ExPASy TrEMBL
Match:
Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)
HSP 1 Score: 891.3 bits (2302), Expect = 2.0e-255
Identity = 432/591 (73.10%), Postives = 475/591 (80.37%), Query Frame = 0
Query: 1 MAKLAKQPLFPMPFLVIFFLLSIISGFGITLSEA-VSKTRHYKWEIEYMFWSPDCAENIV 60
MAK+A +P FP PFLV FF+LSII GFGITLSEA K RHYKWE+EYMFWSPDC ENIV
Sbjct: 1 MAKVADKPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPRPTIRANVGDTIVVEMINKLSTEGV--------------------------- 120
MGINGQFP PTIRAN GD +VVE+ NKL TEGV
Sbjct: 61 MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 -------------AGTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDW 180
AGTYFYHGHLGMQ +A L GSLIVD +E EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180
Query: 181 WHKTVQKQEVGLNSNPMRWINEPESILINGRGQINCSIAAKYKKGVEQCKLKGSEECAPF 240
WH++V KQEVGL+S PMRWI EP+SILINGRGQ +CSIAAKYK G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSV 300
IL V P K YRIRIASTT+L++LNFAIGNH+LLVVEADGNY+QPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300
Query: 301 LITTNQTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSK 360
LITT+Q P ENYWVSIGVR RLPKTPP LT+LNYLPNSV+KLPISPPPETP W+D+DRSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360
Query: 361 NFTNRILATMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIG 420
NFT RI A MGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSL+LPPTPYLAA++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420
Query: 421 LNNTFDHNPPPETFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSE 480
L+ F+ NPPPETFP DYDINNPP N T TGNGVY+F +GE VDVILQNAN L SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480
Query: 481 IHPWHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYGEGKFS D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGTAKALINNPTKKP 551
W FHCHIEPHLHMGMGV+FAEGVHKVG IP A+ACG T A+I N +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGST--AMIKNYPRKP 587
BLAST of HG10007403 vs. TAIR 10
Match:
AT5G21105.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 684.9 bits (1766), Expect = 5.4e-197
Identity = 322/570 (56.49%), Postives = 405/570 (71.05%), Query Frame = 0
Query: 17 IFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGINGQFPRPTIRANVG 76
+++++ +++ T S AV R Y WE+EY +WSPDC E VM +NG+FP PTI+A G
Sbjct: 19 VWWIVLVVAVLTHTASAAV---REYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAG 78
Query: 77 DTIVVEMINKLSTEGVA----------------------------------------GTY 136
DTIVV + NKL+TEG+ GT+
Sbjct: 79 DTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTH 138
Query: 137 FYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNSNPM 196
FYHGH GMQ +A L GSLIVD+ + K E YD + NLLLSDWWH+ + QE+GL+S PM
Sbjct: 139 FYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPM 198
Query: 197 RWINEPESILINGRGQINCSIAAKYKK--GVEQCKLKGSEECAPFILKVKPNKTYRIRIA 256
RWI E +SILINGRGQ NCS+AA++ + C K ++CAP IL V+PNKTYRIR++
Sbjct: 199 RWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLS 258
Query: 257 STTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTNQTPLENYWVS 316
STT+L++LN A+ H+L+VVEADGNYI PF T DIDIYSGESYSVL+TT+Q P +NY++S
Sbjct: 259 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 318
Query: 317 IGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFTNRILATMGSPKP 376
+GVRGR P T ALT+LNY+ +KLP SPPP TP+WDD++RSKNF+ +I + MGSP P
Sbjct: 319 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSP 378
Query: 377 PVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNNTFDHNPPPETFP 436
P K +R+ LLNTQN I+ G+ KWAINNVSL P TPYL +++ L F+ PP ++
Sbjct: 379 PKKYRKRLILLNTQNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYR 438
Query: 437 EDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHPWHLHGHDFWVLG 496
DYDI NPPP NTTTGNG+Y F VDVI+QNAN L+ SEIHPWHLHGHDFWVLG
Sbjct: 439 MDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLG 498
Query: 497 YGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGM 545
YG+GKF +DEK NLKNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGM
Sbjct: 499 YGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGM 558
BLAST of HG10007403 vs. TAIR 10
Match:
AT5G21100.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 649.0 bits (1673), Expect = 3.3e-186
Identity = 315/575 (54.78%), Postives = 403/575 (70.09%), Query Frame = 0
Query: 15 LVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGINGQFPRPTIRAN 74
+++++LL+++ + S AV ++ WE+EY +W PDC E IVM INGQFP PTI A
Sbjct: 3 VIVWWLLTVVVVAFHSASAAVVES---TWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAV 62
Query: 75 VGDTIVVEMINKLSTEGV----------------------------------------AG 134
GDT+++ ++NKLSTEGV AG
Sbjct: 63 AGDTVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAG 122
Query: 135 TYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNSN 194
T+FYHGH GMQ ++ L G LIV KE YD + NLLLSDWWH+++ QE+ L+S
Sbjct: 123 THFYHGHYGMQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELALSSR 182
Query: 195 PMRWINEPESILINGRGQINCSIAAKYKKGVEQ--CKLKGSEECAPFILKVKPNKTYRIR 254
PMRWI EP+S+LINGRGQ NCS AA + KG E+ C K +++CAP L+V+PN+ YR+R
Sbjct: 183 PMRWIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLR 242
Query: 255 IASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTNQTPLENYW 314
IASTT+L++LN A+ HQL+VVEADGNY+ PF +DID+YSGE+YSVL+ TN P + YW
Sbjct: 243 IASTTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYW 302
Query: 315 VSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFTNRILATMGSP 374
+S+GVRGR PKTP ALT++NY+ ++ P PPP TP W+D DRSK+F+ +I A G P
Sbjct: 303 ISVGVRGREPKTPQALTVINYV-DATESRPSHPPPVTPIWNDTDRSKSFSKKIFAAKGYP 362
Query: 375 KPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNNTFD-HNPPPE 434
KPP KS+ ++ LLNTQN + + KW+INNVSLS+P TPYL +IR GL + +D +P +
Sbjct: 363 KPPEKSHDQLILLNTQN-LYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKK 422
Query: 435 TFPEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHPWHLHGHDFW 494
++YDI PPPN NTT G+G+Y F G +VDVILQNAN L+ SEIHPWH+HGHDFW
Sbjct: 423 LIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFW 482
Query: 495 VLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLH 545
VLGYGEGKF +DEK NLKNPPLRNTVV++P+GWTAIRFV DNPGVW FHCHIEPHLH
Sbjct: 483 VLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLH 542
BLAST of HG10007403 vs. TAIR 10
Match:
AT5G21105.3 (Plant L-ascorbate oxidase )
HSP 1 Score: 612.5 bits (1578), Expect = 3.4e-175
Identity = 291/524 (55.53%), Postives = 365/524 (69.66%), Query Frame = 0
Query: 17 IFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGINGQFPRPTIRANVG 76
+++++ +++ T S AV R Y WE+EY +WSPDC E VM +NG+FP PTI+A G
Sbjct: 19 VWWIVLVVAVLTHTASAAV---REYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAG 78
Query: 77 DTIVVEMINKLSTEGVA----------------------------------------GTY 136
DTIVV + NKL+TEG+ GT+
Sbjct: 79 DTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTH 138
Query: 137 FYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNSNPM 196
FYHGH GMQ +A L GSLIVD+ + K E YD + NLLLSDWWH+ + QE+GL+S PM
Sbjct: 139 FYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPM 198
Query: 197 RWINEPESILINGRGQINCSIAAKYKK--GVEQCKLKGSEECAPFILKVKPNKTYRIRIA 256
RWI E +SILINGRGQ NCS+AA++ + C K ++CAP IL V+PNKTYRIR++
Sbjct: 199 RWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLS 258
Query: 257 STTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTNQTPLENYWVS 316
STT+L++LN A+ H+L+VVEADGNYI PF T DIDIYSGESYSVL+TT+Q P +NY++S
Sbjct: 259 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 318
Query: 317 IGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFTNRILATMGSPKP 376
+GVRGR P T ALT+LNY+ +KLP SPPP TP+WDD++RSKNF+ +I + MGSP P
Sbjct: 319 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSP 378
Query: 377 PVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNNTFDHNPPPETFP 436
P K +R+ LLNTQN I+ G+ KWAINNVSL P TPYL +++ L F+ PP ++
Sbjct: 379 PKKYRKRLILLNTQNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYR 438
Query: 437 EDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHPWHLHGHDFWVLG 496
DYDI NPPP NTTTGNG+Y F VDVI+QNAN L+ SEIHPWHLHGHDFWVLG
Sbjct: 439 MDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLG 498
Query: 497 YGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPG 499
YG+GKF +DEK NLKNPPLRNT +++PYGWTAIRFV DNPG
Sbjct: 499 YGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538
BLAST of HG10007403 vs. TAIR 10
Match:
AT5G21105.2 (Plant L-ascorbate oxidase )
HSP 1 Score: 530.0 bits (1364), Expect = 2.2e-150
Identity = 242/384 (63.02%), Postives = 300/384 (78.12%), Query Frame = 0
Query: 163 ESILINGRGQINCSIAAKYKK--GVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLS 222
+SILINGRGQ NCS+AA++ + C K ++CAP IL V+PNKTYRIR++STT+L+
Sbjct: 11 KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70
Query: 223 ALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTNQTPLENYWVSIGVRGR 282
+LN A+ H+L+VVEADGNYI PF T DIDIYSGESYSVL+TT+Q P +NY++S+GVRGR
Sbjct: 71 SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130
Query: 283 LPKTPPALTLLNYLPNSVTKLPISPPPETPKWDDYDRSKNFTNRILATMGSPKPPVKSNR 342
P T ALT+LNY+ +KLP SPPP TP+WDD++RSKNF+ +I + MGSP PP K +
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRK 190
Query: 343 RIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIGLNNTFDHNPPPETFPEDYDIN 402
R+ LLNTQN I+ G+ KWAINNVSL P TPYL +++ L F+ PP ++ DYDI
Sbjct: 191 RLILLNTQNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIM 250
Query: 403 NPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKRSEIHPWHLHGHDFWVLGYGEGKF 462
NPPP NTTTGNG+Y F VDVI+QNAN L+ SEIHPWHLHGHDFWVLGYG+GKF
Sbjct: 251 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 310
Query: 463 SGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAE 522
+DEK NLKNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGVVFAE
Sbjct: 311 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 370
Query: 523 GVHKVGRIPTKALACGGTAKALIN 545
G++++G++P +AL CG T + L+N
Sbjct: 371 GLNRIGKVPDEALGCGLTKQFLMN 393
BLAST of HG10007403 vs. TAIR 10
Match:
AT4G39830.1 (Cupredoxin superfamily protein )
HSP 1 Score: 483.4 bits (1243), Expect = 2.4e-136
Identity = 256/583 (43.91%), Postives = 337/583 (57.80%), Query Frame = 0
Query: 9 LFPMPFLVIFFLLSIISGFGITLSEAVSKTRHYKWEIEYMFWSPDCAENIVMGINGQFPR 68
L + F+ +FF + G K R +KWE++Y F SPDC E +V+ ING+FP
Sbjct: 16 LMVLCFIALFFSSVLCQG----------KIRRFKWEVKYEFKSPDCFEKLVITINGKFPG 75
Query: 69 PTIRANVGDTIVVEMINKLSTEGVA----------------------------------- 128
PTI+A GDTIVVE+ N TE VA
Sbjct: 76 PTIKAQQGDTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQF 135
Query: 129 -----GTYFYHGHLGMQIAARLNGSLIVDLEEEKKEPFHYDEDINLLLSDWWHKTVQKQE 188
GTY YH H GMQ + L G + V + EPF YD D N LL+DW+HK++ ++
Sbjct: 136 VVDRPGTYMYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKA 195
Query: 189 VGLNSNPMRWINEPESILINGRGQINCS----IAAKYKKGVEQCKLKGSEECAPFILKVK 248
GL S P +W+ EP+S++I GRG+ NCS GV C + + +C+ FIL V
Sbjct: 196 TGLASIPFKWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGV--CNVSNA-DCSRFILTVI 255
Query: 249 PNKTYRIRIASTTSLSALNFAIGNHQLLVVEADGNYIQPFLTSDIDIYSGESYSVLITTN 308
P KTYR+RI S T+LSAL+F I H L VVEADG+Y++PF ++ +YSGE+YSVL+ +
Sbjct: 256 PGKTYRLRIGSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKAD 315
Query: 309 QTPLENYWVSIGVRGRLPKTPPALTLLNYLPNSVTKLPISPPPET----PKWDDYDRSKN 368
Q P NYW++ + R TPPA +LNY PN + P P E+ P+W+D
Sbjct: 316 QNPRRNYWITSSIVSRPATTPPATAVLNYYPNHPRRRP--PTSESSNIVPEWNDTRSRLA 375
Query: 369 FTNRILATMGSPKP-PVKSNRRIFLLNTQNHINGGFVKWAINNVSLSLPPTPYLAAIRIG 428
+ I A G P S++ I LLNTQN +N G+ +W++NNVS P TPYL A++
Sbjct: 376 QSLAIKARRGFIHALPENSDKVIVLLNTQNEVN-GYRRWSVNNVSYHHPKTPYLIALKQN 435
Query: 429 LNNTFDHN-PPPETF-PEDYDINNPPPNMNTTTGNGVYQFKLGEIVDVILQNANTLENKR 488
L N FD PE + +YDI P N N TT +G+Y+ + VDVILQNANT+
Sbjct: 436 LTNAFDWRFTAPENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANN 495
Query: 489 SEIHPWHLHGHDFWVLGYGEGKFSGDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNP 541
SE HPWHLHGHDFWVLGYGEGKF+ D K+ N +P +NTV + P+GWTA+RF ADNP
Sbjct: 496 SETHPWHLHGHDFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNP 555
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| P14133 | 2.4e-258 | 72.93 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1 | [more] |
| P24792 | 7.8e-249 | 71.95 | L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2 | [more] |
| P37064 | 5.8e-244 | 72.41 | L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1 | [more] |
| Q40588 | 8.7e-208 | 60.38 | L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1 | [more] |
| M4DUF2 | 9.3e-194 | 57.30 | L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9M5B5 | 1.9e-274 | 77.38 | L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1 | [more] |
| A0A5D3CF11 | 3.6e-273 | 77.38 | L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G0... | [more] |
| A0A5A7UMY3 | 4.0e-256 | 73.27 | L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G... | [more] |
| A0A0A0LXB9 | 8.9e-256 | 72.93 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1 | [more] |
| Q9M5B4 | 2.0e-255 | 73.10 | L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1 | [more] |
