HG10007247 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10007247
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Cytokinin dehydrogenase
Location	Chr10: 2979238 .. 2979933 (-)
RNA-Seq Expression	HG10007247
Synteny	HG10007247

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCTCCTAAACTTTTTCTTCTCATAATTTTCACTTGTTTTCTTTTCCTTCAAAACGCACCGTTTTTTGCAGTGGCTCTCAATTTAGCTCTTCCAAAAGCAATCTTAGAGAAGCTTAAAAATGACCCTCAAACCATTGGCTTAGCTTCCACAGATTATGGCCGGATTGTTCAAGAGAACCCTTATGGCGTTTTCTTTCCGGGGACCGGTCACGACATCGCCGGGCTGATCCGGTTCACGTATGGTAGCTCGGTACCGTTGGATATTGCTGCCCGGGGTCAAGGCCATTGTGTCCGGGGACAAGCCTTGGTGGAAAATGGAGTCGTCGTAAACATGACGTCGCTCGGTGGTTTCGGACAAAAGGCAAGTAGAATTGTGGTGTCGAAAACGTCACCGTTGGGTCCGTACGCTGACGTTGGTGCCGAGCAGCTTTGGATCGATGTGTTACATGCGACAATGCAGAAGGGATTTAGCCCCGTCTCGTGGACGGATTATTTGCGTATCACCGTCGGCGGGACATTGTCCAATGCTGGAATTAGCGGCCAAACGTTTCGGTTCGGTCCTCAAATCAACAATGTTCACGAACTTGACGTTGTAACTGGTCTCTCTCTCAACTCTTTTACAATTTTATATGAAAGTTTAGTTGAACACAATTATATATGCAAATGGGAGATTCAAATCATCATCTTATAA

mRNA sequence

ATGGAAGCTCCTAAACTTTTTCTTCTCATAATTTTCACTTGTTTTCTTTTCCTTCAAAACGCACCGTTTTTTGCAGTGGCTCTCAATTTAGCTCTTCCAAAAGCAATCTTAGAGAAGCTTAAAAATGACCCTCAAACCATTGGCTTAGCTTCCACAGATTATGGCCGGATTGTTCAAGAGAACCCTTATGGCGTTTTCTTTCCGGGGACCGGTCACGACATCGCCGGGCTGATCCGGTTCACGTATGGTAGCTCGGTACCGTTGGATATTGCTGCCCGGGGTCAAGGCCATTGTGTCCGGGGACAAGCCTTGGTGGAAAATGGAGTCGTCGTAAACATGACGTCGCTCGGTGGTTTCGGACAAAAGGCAAGTAGAATTGTGGTGTCGAAAACGTCACCGTTGGGTCCGTACGCTGACGTTGGTGCCGAGCAGCTTTGGATCGATGTGTTACATGCGACAATGCAGAAGGGATTTAGCCCCGTCTCGTGGACGGATTATTTGCGTATCACCGTCGGCGGGACATTGTCCAATGCTGGAATTAGCGGCCAAACGTTTCGGTTCGGTCCTCAAATCAACAATGTTCACGAACTTGACGTTGTAACTGGTCTCTCTCTCAACTCTTTTACAATTTTATATGAAAGTTTAGTTGAACACAATTATATATGCAAATGGGAGATTCAAATCATCATCTTATAA

Coding sequence (CDS)

ATGGAAGCTCCTAAACTTTTTCTTCTCATAATTTTCACTTGTTTTCTTTTCCTTCAAAACGCACCGTTTTTTGCAGTGGCTCTCAATTTAGCTCTTCCAAAAGCAATCTTAGAGAAGCTTAAAAATGACCCTCAAACCATTGGCTTAGCTTCCACAGATTATGGCCGGATTGTTCAAGAGAACCCTTATGGCGTTTTCTTTCCGGGGACCGGTCACGACATCGCCGGGCTGATCCGGTTCACGTATGGTAGCTCGGTACCGTTGGATATTGCTGCCCGGGGTCAAGGCCATTGTGTCCGGGGACAAGCCTTGGTGGAAAATGGAGTCGTCGTAAACATGACGTCGCTCGGTGGTTTCGGACAAAAGGCAAGTAGAATTGTGGTGTCGAAAACGTCACCGTTGGGTCCGTACGCTGACGTTGGTGCCGAGCAGCTTTGGATCGATGTGTTACATGCGACAATGCAGAAGGGATTTAGCCCCGTCTCGTGGACGGATTATTTGCGTATCACCGTCGGCGGGACATTGTCCAATGCTGGAATTAGCGGCCAAACGTTTCGGTTCGGTCCTCAAATCAACAATGTTCACGAACTTGACGTTGTAACTGGTCTCTCTCTCAACTCTTTTACAATTTTATATGAAAGTTTAGTTGAACACAATTATATATGCAAATGGGAGATTCAAATCATCATCTTATAA

Protein sequence

MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQENPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFGQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGISGQTFRFGPQINNVHELDVVTGLSLNSFTILYESLVEHNYICKWEIQIIIL

Homology

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BLAST of HG10007247 vs. NCBI nr
Match: XP_008451152.1 (PREDICTED: cytokinin dehydrogenase 4 [Cucumis melo])

HSP 1 Score: 352.8 bits (904), Expect = 2.2e-93
Identity = 174/202 (86.14%), Postives = 182/202 (90.10%), Query Frame = 0


Query: 1   MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQE 60
           ME+P LFLLIIFTCFLFLQNAPF AVAL+LALPK +L+KLKNDP TI LAS DYG +VQE
Sbjct: 1   MESPNLFLLIIFTCFLFLQNAPFSAVALSLALPKTLLDKLKNDPLTISLASKDYGLMVQE 60

Query: 61  NPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFG 120
           NP GVFFP TGHD+ GLIRF Y S VPL IAARGQGHCVRGQALVENG+VVNMTSLGGF 
Sbjct: 61  NPSGVFFPLTGHDVVGLIRFMYTSPVPLHIAARGQGHCVRGQALVENGLVVNMTSLGGFR 120

Query: 121 QKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGI 180
           QK SRIVVS TSPLGPYADVGAEQLWIDVLHAT QKG SPVSWTDYL ITVGGTLSNAGI
Sbjct: 121 QKTSRIVVSTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWTDYLHITVGGTLSNAGI 180

Query: 181 SGQTFRFGPQINNVHELDVVTG 203
           SGQTFRFGPQINNVHELDV+TG
Sbjct: 181 SGQTFRFGPQINNVHELDVITG 202

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BLAST of HG10007247 vs. NCBI nr
Match: TYK10080.1 (cytokinin dehydrogenase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 352.8 bits (904), Expect = 2.2e-93
Identity = 174/202 (86.14%), Postives = 182/202 (90.10%), Query Frame = 0


Query: 1   MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQE 60
           ME+P LFLLIIFTCFLFLQNAPF AVAL+LALPK +L+KLKNDP TI LAS DYG +VQE
Sbjct: 1   MESPNLFLLIIFTCFLFLQNAPFSAVALSLALPKTLLDKLKNDPLTISLASKDYGLMVQE 60

Query: 61  NPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFG 120
           NP GVFFP TGHD+ GLIRF Y S VPL IAARGQGHCVRGQALVENG+VVNMTSLGGF 
Sbjct: 61  NPSGVFFPLTGHDVVGLIRFMYTSPVPLHIAARGQGHCVRGQALVENGLVVNMTSLGGFR 120

Query: 121 QKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGI 180
           QK SRIVVS TSPLGPYADVGAEQLWIDVLHAT QKG SPVSWTDYL ITVGGTLSNAGI
Sbjct: 121 QKTSRIVVSTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWTDYLHITVGGTLSNAGI 180

Query: 181 SGQTFRFGPQINNVHELDVVTG 203
           SGQTFRFGPQINNVHELDV+TG
Sbjct: 181 SGQTFRFGPQINNVHELDVITG 202

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BLAST of HG10007247 vs. NCBI nr
Match: KAA0055827.1 (cytokinin dehydrogenase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 351.7 bits (901), Expect = 4.9e-93
Identity = 173/202 (85.64%), Postives = 182/202 (90.10%), Query Frame = 0


Query: 1   MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQE 60
           ME+P LFLLIIFTC+LFLQNAPF AVAL+LALPK +L+KLKNDP TI LAS DYG +VQE
Sbjct: 1   MESPNLFLLIIFTCYLFLQNAPFSAVALSLALPKTLLDKLKNDPLTISLASKDYGLMVQE 60

Query: 61  NPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFG 120
           NP GVFFP TGHD+ GLIRF Y S VPL IAARGQGHCVRGQALVENG+VVNMTSLGGF 
Sbjct: 61  NPSGVFFPLTGHDVVGLIRFMYTSPVPLHIAARGQGHCVRGQALVENGLVVNMTSLGGFR 120

Query: 121 QKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGI 180
           QK SRIVVS TSPLGPYADVGAEQLWIDVLHAT QKG SPVSWTDYL ITVGGTLSNAGI
Sbjct: 121 QKTSRIVVSTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWTDYLHITVGGTLSNAGI 180

Query: 181 SGQTFRFGPQINNVHELDVVTG 203
           SGQTFRFGPQINNVHELDV+TG
Sbjct: 181 SGQTFRFGPQINNVHELDVITG 202

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BLAST of HG10007247 vs. NCBI nr
Match: XP_004144113.1 (cytokinin dehydrogenase 2 [Cucumis sativus] >KAE8653432.1 hypothetical protein Csa_006855 [Cucumis sativus])

HSP 1 Score: 329.3 bits (843), Expect = 2.6e-86
Identity = 165/203 (81.28%), Postives = 175/203 (86.21%), Query Frame = 0


Query: 1   MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQE 60
           M+AP  FLLIIFTCFLFLQNAPFF VA ++A PKAI +KLK D QTI LAS DYG +VQ 
Sbjct: 1   MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQH 60

Query: 61  NPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALV-ENGVVVNMTSLGGF 120
           NPY VFFP TGHD+AGLIRF Y   VPL IAARGQGHCVRGQALV +NGVVVNMTSLGG 
Sbjct: 61  NPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGL 120

Query: 121 GQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAG 180
            QK SRIVV+ TSPLGPYADVGAEQLWIDVLHAT QKG SPVSW DYL ITVGGTLSNAG
Sbjct: 121 RQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAG 180

Query: 181 ISGQTFRFGPQINNVHELDVVTG 203
           ISGQTFRFGPQINNV+ELDV+TG
Sbjct: 181 ISGQTFRFGPQINNVYELDVITG 203

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BLAST of HG10007247 vs. NCBI nr
Match: XP_038879415.1 (cytokinin dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 328.6 bits (841), Expect = 4.5e-86
Identity = 162/178 (91.01%), Postives = 166/178 (93.26%), Query Frame = 0


Query: 25  AVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQENPYGVFFPGTGHDIAGLIRFTYGS 84
           +VAL+LALPKAILEKL+NDPQTI LASTDYGRIVQENPYGVFFP TGHDIAGLIRF Y S
Sbjct: 55  SVALSLALPKAILEKLRNDPQTISLASTDYGRIVQENPYGVFFPVTGHDIAGLIRFMYAS 114

Query: 85  SVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFGQKASRIVVSKTSPLGPYADVGAEQ 144
            VP DIAARGQGHCVRGQALVENGVVVNMTSLG F QK SRIVVS TS LGPYADVGAEQ
Sbjct: 115 PVPWDIAARGQGHCVRGQALVENGVVVNMTSLGVFRQKTSRIVVSTTSQLGPYADVGAEQ 174

Query: 145 LWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGISGQTFRFGPQINNVHELDVVTG 203
           LWIDVLHATMQKGFSPVSWTDYL ITVGGTLSNAGISGQTFRFGPQI+NVHELDVVTG
Sbjct: 175 LWIDVLHATMQKGFSPVSWTDYLHITVGGTLSNAGISGQTFRFGPQISNVHELDVVTG 232

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BLAST of HG10007247 vs. ExPASy Swiss-Prot
Match: Q5Z620 (Cytokinin dehydrogenase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX10 PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 7.5e-44
Identity = 92/169 (54.44%), Postives = 108/169 (63.91%), Query Frame = 0


Query: 36  ILEKLKNDPQTIGLASTDYGRIVQENPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQ 95
           I+ K+  D      AS+D+G IV   P GVF P    DIA LIR +     P  +A RG+
Sbjct: 42  IMSKIHTDHDATTKASSDFGHIVHATPNGVFRPTFPADIAALIRLSLSQPTPFTVAPRGK 101

Query: 96  GHCVRGQALVENGVVVNMTSLGGFGQKAS-RIVVSKTSPLGPYADVGAEQLWIDVLHATM 155
           GH  RGQA    G+VV+M++LG  G   S RI VS       Y D G EQLWIDVLH  +
Sbjct: 102 GHSSRGQAFAPGGIVVDMSALGDHGHHTSHRIDVSVDR---MYVDAGGEQLWIDVLHTAL 161

Query: 156 QKGFSPVSWTDYLRITVGGTLSNAGISGQTFRFGPQINNVHELDVVTGL 204
           + G +P  WTDYLRITVGGTLSNAGI GQ FR GPQI+NVHELDVVTG+
Sbjct: 162 KHGLTPRVWTDYLRITVGGTLSNAGIGGQAFRHGPQISNVHELDVVTGM 207

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BLAST of HG10007247 vs. ExPASy Swiss-Prot
Match: Q0JQ12 (Cytokinin dehydrogenase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX1 PE=3 SV=2)

HSP 1 Score: 172.6 bits (436), Expect = 5.4e-42
Identity = 89/165 (53.94%), Postives = 109/165 (66.06%), Query Frame = 0


Query: 39  KLKNDPQTIGLASTDYGRIVQENPYGVFFPGTGHDIAGLIRFTYGS-SVPLDIAARGQGH 98
           KL+ DP     AS D+G I    P  V FPG+  D+A L+R  Y +   P  ++ RG+GH
Sbjct: 46  KLRTDPNATVPASMDFGNITAALPAAVLFPGSPGDVAELLRAAYAAPGRPFTVSFRGRGH 105

Query: 99  CVRGQALVENGVVVNMTSLGGFGQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKG 158
              GQAL   GVVV+M S+GG G  A RI VS     G Y D G EQLW+DVL A + +G
Sbjct: 106 STMGQALAAGGVVVHMQSMGGGG--APRINVSAD---GAYVDAGGEQLWVDVLRAALARG 165

Query: 159 FSPVSWTDYLRITVGGTLSNAGISGQTFRFGPQINNVHELDVVTG 203
            +P SWTDYL +TVGGTLSNAG+SGQT+R GPQI+NV ELDV+TG
Sbjct: 166 VAPRSWTDYLHLTVGGTLSNAGVSGQTYRHGPQISNVLELDVITG 205

[Close]

BLAST of HG10007247 vs. ExPASy Swiss-Prot
Match: Q6YW51 (Cytokinin dehydrogenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX6 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.2e-41
Identity = 94/192 (48.96%), Postives = 117/192 (60.94%), Query Frame = 0


Query: 17  FLQNAPFFAVALNLALP-----KAILEKLKNDPQTIGLASTDYGRIVQENPYGVFFPGTG 76
           F+  A   +V ++  LP      ++  KL+ D  T   AS+D+GRIV   P  V  P T 
Sbjct: 9   FMVMASCLSVVVSGGLPGDLFAHSVASKLRVDRDTTARASSDFGRIVAAAPEAVLHPATP 68

Query: 77  HDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFGQKASRIVVSK- 136
            +IA L+RF+  S  P  +A RGQGH  RGQ+L   GVVV+M +L     +  R+ VS  
Sbjct: 69  AEIAELVRFSASSPSPFPVAPRGQGHSARGQSLAPGGVVVDMRALAA---RRGRVNVSAG 128

Query: 137 TSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGISGQTFRFGPQ 196
            +   PY D G EQLW DVL AT++ G +P  WTDYLRITV GTLSNAGI GQ FR GPQ
Sbjct: 129 GAGAAPYVDAGGEQLWADVLRATLEHGLAPRVWTDYLRITVAGTLSNAGIGGQAFRHGPQ 188

Query: 197 INNVHELDVVTG 203
           I NV ELDV+TG
Sbjct: 189 IANVLELDVITG 197

[Close]

BLAST of HG10007247 vs. ExPASy Swiss-Prot
Match: Q6YW50 (Cytokinin dehydrogenase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX7 PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.6e-41
Identity = 88/168 (52.38%), Postives = 108/168 (64.29%), Query Frame = 0


Query: 35  AILEKLKNDPQTIGLASTDYGRIVQENPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARG 94
           ++  KL+ D  +   AS+D+GRIV   P  V  P T  +IA L+RF+  S  P  +A RG
Sbjct: 32  SVASKLRVDRNSTARASSDFGRIVAAAPEAVLHPATPAEIAELVRFSASSPSPFPVAPRG 91

Query: 95  QGHCVRGQALVENGVVVNMTSLGGFGQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATM 154
           QGH  RGQ+L   GVVV+M +L     +  R+ VS  +   PY D G EQLW DVL AT+
Sbjct: 92  QGHSARGQSLAPGGVVVDMRAL---ASRRGRVNVS--AGAAPYVDAGGEQLWADVLRATL 151

Query: 155 QKGFSPVSWTDYLRITVGGTLSNAGISGQTFRFGPQINNVHELDVVTG 203
           + G +P  WTDYLRITV GTLSNAGI GQ FR GPQI NV ELDV+TG
Sbjct: 152 EHGLAPRVWTDYLRITVAGTLSNAGIGGQAFRHGPQIANVLELDVITG 194

[Close]

BLAST of HG10007247 vs. ExPASy Swiss-Prot
Match: Q9FUJ2 (Cytokinin dehydrogenase 4 OS=Arabidopsis thaliana OX=3702 GN=CKX4 PE=1 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 1.3e-40
Identity = 98/210 (46.67%), Postives = 128/210 (60.95%), Query Frame = 0


Query: 6   LFLLIIFTCFLFLQNAPFFAVA---LNLALPKAILEKLKNDPQTIGLASTDYGRIVQENP 65
           L L +I    LF+   P    +   +++ LP ++   +  DP +I  AS D+G I  ENP
Sbjct: 5   LCLSLITLITLFISLTPTLIKSDEGIDVFLPISLNLTVLTDPFSISAASHDFGNITDENP 64

Query: 66  YGVFFPGTGHDIAGLIRF-------TYGSSVP---LDIAARGQGHCVRGQALVENGVVVN 125
             V  P +  ++A L+RF         GS+ P     +AARGQGH +RGQA    GVVVN
Sbjct: 65  GAVLCPSSTTEVARLLRFANGGFSYNKGSTSPASTFKVAARGQGHSLRGQASAPGGVVVN 124

Query: 126 MTSLGGFGQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVG 185
           MT L     K + +V+S     G YADV A  +W+DVL A + +G SPV+WTDYL ++VG
Sbjct: 125 MTCL-AMAAKPAAVVISAD---GTYADVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVG 184

Query: 186 GTLSNAGISGQTFRFGPQINNVHELDVVTG 203
           GTLSNAGI GQTFR GPQI+NVHELDV+TG
Sbjct: 185 GTLSNAGIGGQTFRHGPQISNVHELDVITG 210

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BLAST of HG10007247 vs. ExPASy TrEMBL
Match: A0A5D3CFE8 (Cytokinin dehydrogenase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002200 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.1e-93
Identity = 174/202 (86.14%), Postives = 182/202 (90.10%), Query Frame = 0


Query: 1   MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQE 60
           ME+P LFLLIIFTCFLFLQNAPF AVAL+LALPK +L+KLKNDP TI LAS DYG +VQE
Sbjct: 1   MESPNLFLLIIFTCFLFLQNAPFSAVALSLALPKTLLDKLKNDPLTISLASKDYGLMVQE 60

Query: 61  NPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFG 120
           NP GVFFP TGHD+ GLIRF Y S VPL IAARGQGHCVRGQALVENG+VVNMTSLGGF 
Sbjct: 61  NPSGVFFPLTGHDVVGLIRFMYTSPVPLHIAARGQGHCVRGQALVENGLVVNMTSLGGFR 120

Query: 121 QKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGI 180
           QK SRIVVS TSPLGPYADVGAEQLWIDVLHAT QKG SPVSWTDYL ITVGGTLSNAGI
Sbjct: 121 QKTSRIVVSTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWTDYLHITVGGTLSNAGI 180

Query: 181 SGQTFRFGPQINNVHELDVVTG 203
           SGQTFRFGPQINNVHELDV+TG
Sbjct: 181 SGQTFRFGPQINNVHELDVITG 202

[Close]

BLAST of HG10007247 vs. ExPASy TrEMBL
Match: A0A1S3BQU4 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103492529 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.1e-93
Identity = 174/202 (86.14%), Postives = 182/202 (90.10%), Query Frame = 0


Query: 1   MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQE 60
           ME+P LFLLIIFTCFLFLQNAPF AVAL+LALPK +L+KLKNDP TI LAS DYG +VQE
Sbjct: 1   MESPNLFLLIIFTCFLFLQNAPFSAVALSLALPKTLLDKLKNDPLTISLASKDYGLMVQE 60

Query: 61  NPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFG 120
           NP GVFFP TGHD+ GLIRF Y S VPL IAARGQGHCVRGQALVENG+VVNMTSLGGF 
Sbjct: 61  NPSGVFFPLTGHDVVGLIRFMYTSPVPLHIAARGQGHCVRGQALVENGLVVNMTSLGGFR 120

Query: 121 QKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGI 180
           QK SRIVVS TSPLGPYADVGAEQLWIDVLHAT QKG SPVSWTDYL ITVGGTLSNAGI
Sbjct: 121 QKTSRIVVSTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWTDYLHITVGGTLSNAGI 180

Query: 181 SGQTFRFGPQINNVHELDVVTG 203
           SGQTFRFGPQINNVHELDV+TG
Sbjct: 181 SGQTFRFGPQINNVHELDVITG 202

[Close]

BLAST of HG10007247 vs. ExPASy TrEMBL
Match: A0A5A7UMK5 (Cytokinin dehydrogenase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G002220 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.4e-93
Identity = 173/202 (85.64%), Postives = 182/202 (90.10%), Query Frame = 0


Query: 1   MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQE 60
           ME+P LFLLIIFTC+LFLQNAPF AVAL+LALPK +L+KLKNDP TI LAS DYG +VQE
Sbjct: 1   MESPNLFLLIIFTCYLFLQNAPFSAVALSLALPKTLLDKLKNDPLTISLASKDYGLMVQE 60

Query: 61  NPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFG 120
           NP GVFFP TGHD+ GLIRF Y S VPL IAARGQGHCVRGQALVENG+VVNMTSLGGF 
Sbjct: 61  NPSGVFFPLTGHDVVGLIRFMYTSPVPLHIAARGQGHCVRGQALVENGLVVNMTSLGGFR 120

Query: 121 QKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGI 180
           QK SRIVVS TSPLGPYADVGAEQLWIDVLHAT QKG SPVSWTDYL ITVGGTLSNAGI
Sbjct: 121 QKTSRIVVSTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWTDYLHITVGGTLSNAGI 180

Query: 181 SGQTFRFGPQINNVHELDVVTG 203
           SGQTFRFGPQINNVHELDV+TG
Sbjct: 181 SGQTFRFGPQINNVHELDVITG 202

[Close]

BLAST of HG10007247 vs. ExPASy TrEMBL
Match: A0A0A0LYW7 (FAD-binding PCMH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589070 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.5e-87
Identity = 170/219 (77.63%), Postives = 182/219 (83.11%), Query Frame = 0


Query: 1   MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQE 60
           M+AP  FLLIIFTCFLFLQNAPFF VA ++A PKAI +KLK D QTI LAS DYG +VQ 
Sbjct: 1   MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQH 60

Query: 61  NPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALV-ENGVVVNMTSLGGF 120
           NPY VFFP TGHD+AGLIRF Y   VPL IAARGQGHCVRGQALV +NGVVVNMTSLGG 
Sbjct: 61  NPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGL 120

Query: 121 GQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAG 180
            QK SRIVV+ TSPLGPYADVGAEQLWIDVLHAT QKG SPVSW DYL ITVGGTLSNAG
Sbjct: 121 RQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAG 180

Query: 181 ISGQTFRFGPQINNVHELDVVTGLSL--NSFTILYESLV 217
           ISGQTFRFGPQINNV+ELDV+TG     +SFT  Y  L+
Sbjct: 181 ISGQTFRFGPQINNVYELDVITGTYFFNDSFTFFYILLI 219

[Close]

BLAST of HG10007247 vs. ExPASy TrEMBL
Match: A0A6J1HW04 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111466785 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.4e-77
Identity = 152/202 (75.25%), Postives = 165/202 (81.68%), Query Frame = 0


Query: 1   MEAPKLFLLIIFTCFLFLQNAPFFAVALNLALPKAILEKLKNDPQTIGLASTDYGRIVQE 60
           M  P LFLLIIFT FL L NAPF A A NL+LP+++ EKL++D QTI LASTDYGRI+QE
Sbjct: 1   MVFPNLFLLIIFTSFLSLHNAPFRASADNLSLPESLAEKLRDDLQTISLASTDYGRIIQE 60

Query: 61  NPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHCVRGQALVENGVVVNMTSLGGFG 120
           NP GVFFP TG+DIA  IR  Y   + LDIAARGQGH VRGQAL  NGVV+NMTSL    
Sbjct: 61  NPIGVFFPVTGNDIAVFIRVMYAGPMRLDIAARGQGHSVRGQALAGNGVVMNMTSLANSQ 120

Query: 121 QKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVGGTLSNAGI 180
           Q   RIVV  TSPLGPYADVG EQLWIDVLH TM+KGFSPVSWTDYL ITVGGTLSNAGI
Sbjct: 121 QGGGRIVVKTTSPLGPYADVGGEQLWIDVLHETMRKGFSPVSWTDYLHITVGGTLSNAGI 180

Query: 181 SGQTFRFGPQINNVHELDVVTG 203
           SGQTFRFGPQI+NV+ELDVVTG
Sbjct: 181 SGQTFRFGPQISNVYELDVVTG 202

[Close]

BLAST of HG10007247 vs. TAIR 10
Match: AT4G29740.2 (cytokinin oxidase 4 )

HSP 1 Score: 167.9 bits (424), Expect = 9.4e-42
Identity = 98/210 (46.67%), Postives = 128/210 (60.95%), Query Frame = 0


Query: 6   LFLLIIFTCFLFLQNAPFFAVA---LNLALPKAILEKLKNDPQTIGLASTDYGRIVQENP 65
           L L +I    LF+   P    +   +++ LP ++   +  DP +I  AS D+G I  ENP
Sbjct: 5   LCLSLITLITLFISLTPTLIKSDEGIDVFLPISLNLTVLTDPFSISAASHDFGNITDENP 64

Query: 66  YGVFFPGTGHDIAGLIRF-------TYGSSVP---LDIAARGQGHCVRGQALVENGVVVN 125
             V  P +  ++A L+RF         GS+ P     +AARGQGH +RGQA    GVVVN
Sbjct: 65  GAVLCPSSTTEVARLLRFANGGFSYNKGSTSPASTFKVAARGQGHSLRGQASAPGGVVVN 124

Query: 126 MTSLGGFGQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVG 185
           MT L     K + +V+S     G YADV A  +W+DVL A + +G SPV+WTDYL ++VG
Sbjct: 125 MTCL-AMAAKPAAVVISAD---GTYADVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVG 184

Query: 186 GTLSNAGISGQTFRFGPQINNVHELDVVTG 203
           GTLSNAGI GQTFR GPQI+NVHELDV+TG
Sbjct: 185 GTLSNAGIGGQTFRHGPQISNVHELDVITG 210

[Close]

BLAST of HG10007247 vs. TAIR 10
Match: AT4G29740.1 (cytokinin oxidase 4 )

HSP 1 Score: 167.9 bits (424), Expect = 9.4e-42
Identity = 98/210 (46.67%), Postives = 128/210 (60.95%), Query Frame = 0


Query: 6   LFLLIIFTCFLFLQNAPFFAVA---LNLALPKAILEKLKNDPQTIGLASTDYGRIVQENP 65
           L L +I    LF+   P    +   +++ LP ++   +  DP +I  AS D+G I  ENP
Sbjct: 5   LCLSLITLITLFISLTPTLIKSDEGIDVFLPISLNLTVLTDPFSISAASHDFGNITDENP 64

Query: 66  YGVFFPGTGHDIAGLIRF-------TYGSSVP---LDIAARGQGHCVRGQALVENGVVVN 125
             V  P +  ++A L+RF         GS+ P     +AARGQGH +RGQA    GVVVN
Sbjct: 65  GAVLCPSSTTEVARLLRFANGGFSYNKGSTSPASTFKVAARGQGHSLRGQASAPGGVVVN 124

Query: 126 MTSLGGFGQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYLRITVG 185
           MT L     K + +V+S     G YADV A  +W+DVL A + +G SPV+WTDYL ++VG
Sbjct: 125 MTCL-AMAAKPAAVVISAD---GTYADVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVG 184

Query: 186 GTLSNAGISGQTFRFGPQINNVHELDVVTG 203
           GTLSNAGI GQTFR GPQI+NVHELDV+TG
Sbjct: 185 GTLSNAGIGGQTFRHGPQISNVHELDVITG 210

[Close]

BLAST of HG10007247 vs. TAIR 10
Match: AT2G19500.1 (cytokinin oxidase 2 )

HSP 1 Score: 167.5 bits (423), Expect = 1.2e-41
Identity = 87/175 (49.71%), Postives = 108/175 (61.71%), Query Frame = 0


Query: 28  LNLALPKAILEKLKNDPQTIGLASTDYGRIVQENPYGVFFPGTGHDIAGLIRFTYGSSVP 87
           + + LPK++   L  DP  I  AS D+G I    P GV  P +  DI+ L+++       
Sbjct: 23  IKIDLPKSLNLTLSTDPSIISAASHDFGNITTVTPGGVICPSSTADISRLLQYAANGKST 82

Query: 88  LDIAARGQGHCVRGQALVENGVVVNMTSLGGFGQKASRIVVSKTSPLGPYADVGAEQLWI 147
             +AARGQGH + GQA V  GV+VNMT +       + +VVSK      YADV A  LW+
Sbjct: 83  FQVAARGQGHSLNGQASVSGGVIVNMTCI-------TDVVVSKDK---KYADVAAGTLWV 142

Query: 148 DVLHATMQKGFSPVSWTDYLRITVGGTLSNAGISGQTFRFGPQINNVHELDVVTG 203
           DVL  T +KG SPVSWTDYL ITVGGTLSN GI GQ FR GP ++NV ELDV+TG
Sbjct: 143 DVLKKTAEKGVSPVSWTDYLHITVGGTLSNGGIGGQVFRNGPLVSNVLELDVITG 187

[Close]

BLAST of HG10007247 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 158.7 bits (400), Expect = 5.7e-39
Identity = 81/164 (49.39%), Postives = 108/164 (65.85%), Query Frame = 0


Query: 39  KLKNDPQTIGLASTDYGRIVQENPYGVFFPGTGHDIAGLIRFTYGSSVPLDIAARGQGHC 98
           KL +   ++  A+TD+G + +  P  V  P +  DI  LI+ ++ S +   +AARG GH 
Sbjct: 47  KLTSSSSSVESAATDFGHVTKIFPSAVLIPSSVEDITDLIKLSFDSQLSFPLAARGHGHS 106

Query: 99  VRGQALVENGVVVNMTSLGGFGQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGF 158
            RGQA  ++GVVVNM S+     +   I VS+T     Y DV A  LWI+VL+ T++ G 
Sbjct: 107 HRGQASAKDGVVVNMRSM---VNRDRGIKVSRTC---LYVDVDAAWLWIEVLNKTLELGL 166

Query: 159 SPVSWTDYLRITVGGTLSNAGISGQTFRFGPQINNVHELDVVTG 203
           +PVSWTDYL +TVGGTLSN GISGQTFR+GPQI NV E+DV+TG
Sbjct: 167 TPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITG 204

[Close]

BLAST of HG10007247 vs. TAIR 10
Match: AT3G63440.1 (cytokinin oxidase/dehydrogenase 6 )

HSP 1 Score: 151.4 bits (381), Expect = 9.1e-37
Identity = 80/155 (51.61%), Postives = 103/155 (66.45%), Query Frame = 0


Query: 50  ASTDYGRIVQENPYGVFFPGTGHDIAGLIRFTY--GSSVPLDIAARGQGHCVRGQALVEN 109
           AS D+G   Q  P  V  P +  DIA  IR  +  G+   L +AARG+GH ++GQA   +
Sbjct: 60  ASKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGHSLQGQAQTRH 119

Query: 110 GVVVNMTSLGGFGQKASRIVVSKTSPLGPYADVGAEQLWIDVLHATMQKGFSPVSWTDYL 169
           G+V++M SL    QK    V S  SP  PY DV   +LWI++LH T++ G +P SWTDYL
Sbjct: 120 GIVIHMESL--HPQKLQ--VYSVDSP-APYVDVSGGELWINILHETLKYGLAPKSWTDYL 179

Query: 170 RITVGGTLSNAGISGQTFRFGPQINNVHELDVVTG 203
            +TVGGTLSNAGISGQ FR GPQI+NVH+L++VTG
Sbjct: 180 HLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTG 209

The following BLAST results are available for this feature:

BLAST of HG10007247 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008451152.1	2.2e-93	86.14	PREDICTED: cytokinin dehydrogenase 4 [Cucumis melo]	[more]
TYK10080.1	2.2e-93	86.14	cytokinin dehydrogenase 4 [Cucumis melo var. makuwa]	[more]
KAA0055827.1	4.9e-93	85.64	cytokinin dehydrogenase 4 [Cucumis melo var. makuwa]	[more]
XP_004144113.1	2.6e-86	81.28	cytokinin dehydrogenase 2 [Cucumis sativus] >KAE8653432.1 hypothetical protein C...	[more]
XP_038879415.1	4.5e-86	91.01	cytokinin dehydrogenase 2-like [Benincasa hispida]	[more]

BLAST of HG10007247 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5Z620	7.5e-44	54.44	Cytokinin dehydrogenase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX10 PE=...	[more]
Q0JQ12	5.4e-42	53.94	Cytokinin dehydrogenase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX1 PE=3 ...	[more]
Q6YW51	1.2e-41	48.96	Cytokinin dehydrogenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX6 PE=3 ...	[more]
Q6YW50	1.6e-41	52.38	Cytokinin dehydrogenase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX7 PE=3 ...	[more]
Q9FUJ2	1.3e-40	46.67	Cytokinin dehydrogenase 4 OS=Arabidopsis thaliana OX=3702 GN=CKX4 PE=1 SV=2	[more]

BLAST of HG10007247 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CFE8	1.1e-93	86.14	Cytokinin dehydrogenase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3BQU4	1.1e-93	86.14	Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103492529 PE=3 SV=1	[more]
A0A5A7UMK5	2.4e-93	85.64	Cytokinin dehydrogenase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A0A0LYW7	1.5e-87	77.63	FAD-binding PCMH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A6J1HW04	2.4e-77	75.25	Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111466785 PE=3 SV=1	[more]

BLAST of HG10007247 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29740.2	9.4e-42	46.67	cytokinin oxidase 4	[more]
AT4G29740.1	9.4e-42	46.67	cytokinin oxidase 4	[more]
AT2G19500.1	1.2e-41	49.71	cytokinin oxidase 2	[more]
AT5G56970.1	5.7e-39	49.39	cytokinin oxidase 3	[more]
AT3G63440.1	9.1e-37	51.61	cytokinin oxidase/dehydrogenase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 64..202 e-value: 2.0E-11 score: 43.8
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 113..202 e-value: 6.9E-61 score: 208.4
IPR016167	FAD-binding, type PCMH, subdomain 1	GENE3D	3.30.43.10		coord: 43..112 e-value: 6.9E-61 score: 208.4
None	No IPR available	PANTHER	PTHR13878:SF127	CYTOKININ DEHYDROGENASE 3	coord: 34..202
None	No IPR available	PANTHER	PTHR13878	GULONOLACTONE OXIDASE	coord: 34..202
IPR006093	Oxygen oxidoreductase covalent FAD-binding site	PROSITE	PS00862	OX2_COVAL_FAD	coord: 62..97
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 42..202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10007247.1	HG10007247.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009690	cytokinin metabolic process
molecular_function	GO:0019139	cytokinin dehydrogenase activity
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016491	oxidoreductase activity