HG10007097 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10007097 vs. NCBI nr
Match: XP_038878345.1 (amidase 1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 769.6 bits (1986), Expect = 1.4e-218
Identity = 387/431 (89.79%), Postives = 404/431 (93.74%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAVQDHGAFIEKFLLQMSS SDQLPLTGLTFAVKDIFDI+GYV GFGNPEWLRTH PANQ
Sbjct: 1   MAVQDHGAFIEKFLLQMSSPSDQLPLTGLTFAVKDIFDIEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAF--SINGENFHYGTPQNPYASDRIPGGSSSGSAVA 120
           TAPAVLAIL+GGATCI +TIM E+A+  SINGENFHYGTPQNPYASDRIPGGSSSGS+VA
Sbjct: 61  TAPAVLAILRGGATCIRQTIMMEIAYFASINGENFHYGTPQNPYASDRIPGGSSSGSSVA 120

Query: 121 V---------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSA 180
           V         GTDTGGSVRVPASYCGILGFRPSHG VSTSGVIPMTQSFDTVGWFARDS 
Sbjct: 121 VAAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDST 180

Query: 181 IFKRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQI 240
           I KRVG+LLL+EPE+EHYKPTQV IAEDCFKLSSI SERLTQ+F+NSV+KLFGGHL+KQI
Sbjct: 181 ILKRVGRLLLKEPEIEHYKPTQVFIAEDCFKLSSISSERLTQSFVNSVEKLFGGHLLKQI 240

Query: 241 SLGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSH 300
           SLGNYVED VPSLKHFMI++N+GYKHSIPSLAALVRSKRLLQRHEFKINHGEW+ SY SH
Sbjct: 241 SLGNYVEDNVPSLKHFMIQENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYKSH 300

Query: 301 LGPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTD 360
           LGPGISERILEYI EATDENTDLSRSIQIELREALAAL EDFGVLAIPTVPGPPPKLNTD
Sbjct: 301 LGPGISERILEYITEATDENTDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTD 360

Query: 361 ISEMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLY 420
           ISE YDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLY
Sbjct: 361 ISEQYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLY 420

BLAST of HG10007097 vs. NCBI nr
Match: XP_004135625.1 (amidase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 765.0 bits (1974), Expect = 3.4e-217
Identity = 383/430 (89.07%), Postives = 403/430 (93.72%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAV D+GAFIEKFLLQMSS SD+LPL+GLTFAVKDIFDI+GYVAGFGNPEWLRTH PANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV- 120
           TAPAVL IL+GGATCIGKTIMDEMAFSINGENFHYGTPQNP+ASDRIPGGSSSGS VAV 
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIF 180
                   GTDTGGSVRVPASYCGILGFRPSHG VSTSGVIPMTQSFDTVGWFARDSAI 
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGQLLLQEPEVEHYKPTQVLIAEDCFK-LSSIPSERLTQAFINSVKKLFGGHLIKQIS 240
           KRVG LLLQEPE+EHYKPT+V IAEDCFK LS+I SERLTQAF++SV+KLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHL 300
           LG YVEDKVPSLKHFMIE+N+GYKHSIPSLAALVRSKR LQRHEF INHGEW+RSY+SHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300

Query: 301 GPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISERILE+IRE TDEN DLSRSIQIELREALAAL EDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYS 420
           SE YDFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLN+VDSLYS
Sbjct: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420

BLAST of HG10007097 vs. NCBI nr
Match: KAE8653373.1 (hypothetical protein Csa_007619 [Cucumis sativus])

HSP 1 Score: 762.3 bits (1967), Expect = 2.2e-216
Identity = 383/437 (87.64%), Postives = 403/437 (92.22%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAV D+GAFIEKFLLQMSS SD+LPL+GLTFAVKDIFDI+GYVAGFGNPEWLRTH PANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVA-- 120
           TAPAVL IL+GGATCIGKTIMDEMAFSINGENFHYGTPQNP+ASDRIPGGSSSGS VA  
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 --------------VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWF 180
                         VGTDTGGSVRVPASYCGILGFRPSHG VSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLGTITQFVGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 ARDSAIFKRVGQLLLQEPEVEHYKPTQVLIAEDCFK-LSSIPSERLTQAFINSVKKLFGG 240
           ARDSAI KRVG LLLQEPE+EHYKPT+V IAEDCFK LS+I SERLTQAF++SV+KLFGG
Sbjct: 181 ARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGG 240

Query: 241 HLIKQISLGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWL 300
           HLIKQISLG YVEDKVPSLKHFMIE+N+GYKHSIPSLAALVRSKR LQRHEF INHGEW+
Sbjct: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWV 300

Query: 301 RSYNSHLGPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPP 360
           RSY+SHLGPGISERILE+IRE TDEN DLSRSIQIELREALAAL EDFGVLAIPTVPGPP
Sbjct: 301 RSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPP 360

Query: 361 PKLNTDISEMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLN 420
           PKLNTDISE YDFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLN
Sbjct: 361 PKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLN 420

BLAST of HG10007097 vs. NCBI nr
Match: XP_022968724.1 (amidase 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 760.4 bits (1962), Expect = 8.3e-216
Identity = 377/429 (87.88%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAVQDHGAFIEKF+LQ+SS SD LPL GLTFAVKDIFD++GYV GFGNPEWLRTH PANQ
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV- 120
           TAPAVLAIL+GGATCIGKTIMDEMAFSI+GENFHYGTP+NPYASDRIPGGSSSGSAVAV 
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIF 180
                   GTDTGGSVRVPASYCGILGFRPSHG VSTSGVIPMTQSFDTVGWFA DSA+F
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAIDSAVF 180

Query: 181 KRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQISL 240
           KRVG+LLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAF+NSVKKLFG HLIKQIS+
Sbjct: 181 KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQISI 240

Query: 241 GNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHLG 300
           G+YVEDKVPSLKHFMIE+N+GYKHSIPSLAALVRSK LLQRHEFKINHGEW+RSY+SHLG
Sbjct: 241 GSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVRSYSSHLG 300

Query: 301 PGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDIS 360
           PG SERI EYIR ATDENTDLS S+QIELR ALAAL EDFGVL IPTVPGPPPKLNTDIS
Sbjct: 301 PGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPPKLNTDIS 360

Query: 361 EMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYST 420
           E++DFRAKAFSLL+IA+VSG+CQ +IPLGLYNGLPVSISLLAKHGSDGFLLNVVD+LYST
Sbjct: 361 ELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTLYST 420

BLAST of HG10007097 vs. NCBI nr
Match: XP_022961361.1 (amidase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 758.8 bits (1958), Expect = 2.4e-215
Identity = 377/429 (87.88%), Postives = 401/429 (93.47%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAVQDHGAFIEKF+LQ SS SD LPLTGLTFAVKDIFD++GYV GFGNPEWLRTH PANQ
Sbjct: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV- 120
           TAPAVLAIL+GGATCIGKTIMDEMAFSI+GENFHYGTP+NPYASDRIPGGSSSGSAVAV 
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIF 180
                   GTDTGGSVRVPASYCGILGFRPSHG VSTSGVIPMTQSFDTVGWFA DSA+F
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAIDSAVF 180

Query: 181 KRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQISL 240
           KRVG+LLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAF+NSVKKLFG HLIKQIS+
Sbjct: 181 KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQISI 240

Query: 241 GNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHLG 300
           G+YVEDKVPSLKHFMIE+N+GYKH IPSLAALVRSK LLQRHEFKINHGEW+RSY+SHLG
Sbjct: 241 GSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSSHLG 300

Query: 301 PGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDIS 360
           PG SERI EYIR ATDENTDLSRS+QIELR ALAAL EDFGVL IPTVPGPPPKL TDIS
Sbjct: 301 PGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRTDIS 360

Query: 361 EMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYST 420
           E++DFRAKAFSLL+IA+VSG+CQ +IPLGLYNGLPVSISLLAKHGSDGFLLNVVD+LYST
Sbjct: 361 ELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTLYST 420

BLAST of HG10007097 vs. ExPASy Swiss-Prot
Match: Q9FR37 (Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 5.4e-141
Identity = 258/417 (61.87%), Postives = 313/417 (75.06%), Query Frame = 0

Query: 5   DHGAFIEKFLLQMSSLSDQLP-LTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQTAP 64
           D GAFIEK  +  +S S   P L GLTFA+KDIFD++G V GFGNP+WLRTHS A  TAP
Sbjct: 6   DFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAP 65

Query: 65  AVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV---- 124
            V ++L+ GAT +G TIMDEMA+SINGEN HYGTP+NP A DR+PGGSSSGSAVAV    
Sbjct: 66  VVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARL 125

Query: 125 -----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIFKRV 184
                GTDTGGSVRVPASYCGI GFRPSHGAVST G+ PM QSFDTVGWFARD+A  KRV
Sbjct: 126 VDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKRV 185

Query: 185 GQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGH-LIKQISLGN 244
           G +LLQ+  +   +P+Q++IA+DCFKL S+P + L Q  + SV+K FGG+ ++K+++LG 
Sbjct: 186 GCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNLGE 245

Query: 245 YVEDKVPSLKHFMIEDN--EGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHLG 304
           Y+   VPSLKHFM  D+     +  IPSL AL  S RLLQRHEFKINHG W+ S     G
Sbjct: 246 YIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPEFG 305

Query: 305 PGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDIS 364
           PGISERI E IR  +DE  D  RS++ EL  AL+ L  + GVL IPTVPGPPP L  +++
Sbjct: 306 PGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQANVA 365

Query: 365 EMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSL 409
            +  FR++AFSLL+IA VSG CQVSIPLGL+  LPVS+SL+AK+GSDGFLL++VDSL
Sbjct: 366 ALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of HG10007097 vs. ExPASy Swiss-Prot
Match: Q7XTK3 (Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 8.9e-128
Identity = 233/420 (55.48%), Postives = 297/420 (70.71%), Query Frame = 0

Query: 5   DHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQTAPA 64
           D+GAF+E+F+L     S QLPL GLTFA+KDIFDI G V GFGNP+W RTH+PA  T+P 
Sbjct: 10  DYGAFMERFVLPPPP-SQQLPLHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPV 69

Query: 65  VLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV----- 124
           VLA L  GAT +G TIMDEMA+SINGEN HYGTP NP A  R+PGGSSSGSAVAV     
Sbjct: 70  VLAALAAGATSLGTTIMDEMAYSINGENTHYGTPTNPCAPGRVPGGSSSGSAVAVAANLV 129

Query: 125 ----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIFKRVG 184
               GTDTGGSVRVPA+YCGI G RPSHG VS   VIPM Q FDTVGWF+RD +   RV 
Sbjct: 130 DFSLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDLSTLSRVT 189

Query: 185 QLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQISLGNYV 244
           ++LL  P+    +PTQV I  DCF++     +R  Q    SV K F   ++   +LG+++
Sbjct: 190 KVLLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFDSQILDNRNLGDFI 249

Query: 245 EDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHLGPGIS 304
            D VPS+  F+ + +E    S+P+L+ +    R LQR +FK NH EW+ +   +LGPG+ 
Sbjct: 250 SDNVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKPNLGPGLR 309

Query: 305 ERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDISEMYD 364
           ERILE I    +E+ +  ++I+ E + ALAAL +D G+LAIPTVPGPPPK+  + + + +
Sbjct: 310 ERILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPKVGMEAAPLEN 369

Query: 365 FRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYSTLKEE 416
           FRA+AFSLL+IA +SG CQVSIPLG+ NGLPVS+SL+A+HG+D FLLNVV+ LY TL +E
Sbjct: 370 FRARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEELYQTLIDE 428

BLAST of HG10007097 vs. ExPASy Swiss-Prot
Match: Q9MUK5 (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=TOC64 PE=1 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.4e-109
Identity = 201/427 (47.07%), Postives = 286/427 (66.98%), Query Frame = 0

Query: 4   QDHGAFIEKFLL----QMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPAN 63
           +D GAF+ K  L      +      PL+ L FA+ DIFDI+G+V+ FG+PEW RTH PA+
Sbjct: 35  EDFGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPAS 94

Query: 64  QTAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV 123
            TA AV A+++ GATCIG T++DE+A+ I+GEN H+GTP NP   +R+PGGSSSG+AVAV
Sbjct: 95  STASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAV 154

Query: 124 ---------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAI 183
                    G DT G VRVPA +CGILGFRPSHGAVS  G+IP++ S DTVGWFA+D  +
Sbjct: 155 AANFVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTVGWFAKDPDV 214

Query: 184 FKRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQIS 243
            +RVG +LLQ P V    P Q++IA+DCF+  ++P +R +Q  I + +KLFG  ++K I+
Sbjct: 215 LRRVGHILLQAPFVMQRNPRQIIIADDCFQHLNVPLDRTSQVVIKATEKLFGKQVLKHIN 274

Query: 244 LGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHL 303
             +Y+  KV SLK   I+ + G   S  SL  L    + LQRHEF+  H EW+      L
Sbjct: 275 FEDYISSKVSSLKACSIQKSNGVLKS-SSLKLLANVMQSLQRHEFEHTHSEWMSIVKPDL 334

Query: 304 GPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDI 363
            P +S ++ E   E ++   + S+S++ ELR A+ +L +D GVL IPTV  PPPKL    
Sbjct: 335 HPAVSAQLHEKF-EVSELEIENSKSVRSELRVAVNSLLKDEGVLVIPTVADPPPKLGGKE 394

Query: 364 SEMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYS 418
              +D++++A SLL+IA++SG CQV++PLG ++  PVS+SL+A+HG D FLL+ + ++Y+
Sbjct: 395 FLSHDYQSRALSLLSIASISGCCQVTVPLGFFDKNPVSVSLIARHGGDRFLLDTLKTMYT 454

BLAST of HG10007097 vs. ExPASy Swiss-Prot
Match: Q9LVH5 (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP64 PE=1 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 5.4e-109
Identity = 200/424 (47.17%), Postives = 278/424 (65.57%), Query Frame = 0

Query: 4   QDHGAFIEKFLL----QMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPAN 63
           +D GAFI+K +L    Q +      PLTGLTFAV D+FDI GYV GFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV 123
            T+P V  +++GGATC+GKT++DE AFSI+GEN HY +P NP A  RIPGG+ SG+AVAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 ---------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAI 183
                    G DT G VRVPA YCG+LGF+ S+GA+S +G+IP++ S D+VGWFARD   
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 FKRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQIS 243
            +RVG +LLQ P      P Q+++A+DCF+L  IP +R+TQ    S +KLFG  L+K  +
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGRQLLKHQN 271

Query: 244 LGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHL 303
           L  Y E KVPSLK F           + +   L    +LLQRHEF  NHG+W+ +    +
Sbjct: 272 LETYFETKVPSLKEF-ARTKAIANTKVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPAI 331

Query: 304 GPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDI 363
            P I  ++ E   E T+E T+   +I+ E R A+ +L +D G+L IPT+P  PPKL +  
Sbjct: 332 DPVILSQVCEN-PELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSKE 391

Query: 364 SEMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYS 415
               D++ +A SLL+IA++SG CQV++PLG +   P+S+S + +HG D FLL+ V ++Y 
Sbjct: 392 ITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMYP 451

BLAST of HG10007097 vs. ExPASy Swiss-Prot
Match: F4KCL7 (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 4.4e-95
Identity = 182/428 (42.52%), Postives = 266/428 (62.15%), Query Frame = 0

Query: 4   QDHGAFIEKFLL----QMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPAN 63
           +D GAF+++F L         + +  L+GLTF++ D FD+  Y+ GFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV 123
           +TA  V  +LK GATC+GKTIMDE+ F I GEN HYGTP NP   D +PGG SSGSAV+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 GT---------DTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAI 183
           G          DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA D ++
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 FKRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQIS 243
             +VG  LL    V H +   ++ A+D F+LS IP ++  Q    +++ L G    K ++
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGYKTPKHVN 284

Query: 244 LGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHL 303
           +G YV   VPSL  F  E +   ++S  +L AL      +QRHEFK NH EW ++  S L
Sbjct: 285 VGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSFL 344

Query: 304 GPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDI 363
           GP  S  ++  ++   +    L R ++ E+R  + +L ++ G+L IPTV  PPP+LNT  
Sbjct: 345 GPRFSNDVVTALKSKNESIKSLYR-VKNEMRATIQSLLKEDGILVIPTVADPPPRLNTKR 404

Query: 364 SE-MYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLY 418
           ++ + +F  + ++L  IA++SG CQV+IPLG +   P+S+SLL  +G D FLL+    +Y
Sbjct: 405 NKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDVY 464

BLAST of HG10007097 vs. ExPASy TrEMBL
Match: A0A0A0LZ24 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570120 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 1.6e-217
Identity = 383/430 (89.07%), Postives = 403/430 (93.72%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAV D+GAFIEKFLLQMSS SD+LPL+GLTFAVKDIFDI+GYVAGFGNPEWLRTH PANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV- 120
           TAPAVL IL+GGATCIGKTIMDEMAFSINGENFHYGTPQNP+ASDRIPGGSSSGS VAV 
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIF 180
                   GTDTGGSVRVPASYCGILGFRPSHG VSTSGVIPMTQSFDTVGWFARDSAI 
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGQLLLQEPEVEHYKPTQVLIAEDCFK-LSSIPSERLTQAFINSVKKLFGGHLIKQIS 240
           KRVG LLLQEPE+EHYKPT+V IAEDCFK LS+I SERLTQAF++SV+KLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHL 300
           LG YVEDKVPSLKHFMIE+N+GYKHSIPSLAALVRSKR LQRHEF INHGEW+RSY+SHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300

Query: 301 GPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISERILE+IRE TDEN DLSRSIQIELREALAAL EDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYS 420
           SE YDFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLN+VDSLYS
Sbjct: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420

BLAST of HG10007097 vs. ExPASy TrEMBL
Match: A0A6J1I0H5 (amidase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 4.0e-216
Identity = 377/429 (87.88%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAVQDHGAFIEKF+LQ+SS SD LPL GLTFAVKDIFD++GYV GFGNPEWLRTH PANQ
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV- 120
           TAPAVLAIL+GGATCIGKTIMDEMAFSI+GENFHYGTP+NPYASDRIPGGSSSGSAVAV 
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIF 180
                   GTDTGGSVRVPASYCGILGFRPSHG VSTSGVIPMTQSFDTVGWFA DSA+F
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAIDSAVF 180

Query: 181 KRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQISL 240
           KRVG+LLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAF+NSVKKLFG HLIKQIS+
Sbjct: 181 KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQISI 240

Query: 241 GNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHLG 300
           G+YVEDKVPSLKHFMIE+N+GYKHSIPSLAALVRSK LLQRHEFKINHGEW+RSY+SHLG
Sbjct: 241 GSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVRSYSSHLG 300

Query: 301 PGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDIS 360
           PG SERI EYIR ATDENTDLS S+QIELR ALAAL EDFGVL IPTVPGPPPKLNTDIS
Sbjct: 301 PGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPPKLNTDIS 360

Query: 361 EMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYST 420
           E++DFRAKAFSLL+IA+VSG+CQ +IPLGLYNGLPVSISLLAKHGSDGFLLNVVD+LYST
Sbjct: 361 ELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTLYST 420

BLAST of HG10007097 vs. ExPASy TrEMBL
Match: A0A6J1HA58 (amidase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461951 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.2e-215
Identity = 377/429 (87.88%), Postives = 401/429 (93.47%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAVQDHGAFIEKF+LQ SS SD LPLTGLTFAVKDIFD++GYV GFGNPEWLRTH PANQ
Sbjct: 1   MAVQDHGAFIEKFILQQSSPSDPLPLTGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV- 120
           TAPAVLAIL+GGATCIGKTIMDEMAFSI+GENFHYGTP+NPYASDRIPGGSSSGSAVAV 
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIF 180
                   GTDTGGSVRVPASYCGILGFRPSHG VSTSGVIPMTQSFDTVGWFA DSA+F
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAIDSAVF 180

Query: 181 KRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQISL 240
           KRVG+LLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAF+NSVKKLFG HLIKQIS+
Sbjct: 181 KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQISI 240

Query: 241 GNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHLG 300
           G+YVEDKVPSLKHFMIE+N+GYKH IPSLAALVRSK LLQRHEFKINHGEW+RSY+SHLG
Sbjct: 241 GSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSSHLG 300

Query: 301 PGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDIS 360
           PG SERI EYIR ATDENTDLSRS+QIELR ALAAL EDFGVL IPTVPGPPPKL TDIS
Sbjct: 301 PGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRTDIS 360

Query: 361 EMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYST 420
           E++DFRAKAFSLL+IA+VSG+CQ +IPLGLYNGLPVSISLLAKHGSDGFLLNVVD+LYST
Sbjct: 361 ELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTLYST 420

BLAST of HG10007097 vs. ExPASy TrEMBL
Match: A0A6J1HYY1 (amidase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 9.9e-215
Identity = 377/431 (87.47%), Postives = 402/431 (93.27%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAVQDHGAFIEKF+LQ+SS SD LPL GLTFAVKDIFD++GYV GFGNPEWLRTH PANQ
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV- 120
           TAPAVLAIL+GGATCIGKTIMDEMAFSI+GENFHYGTP+NPYASDRIPGGSSSGSAVAV 
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIF 180
                   GTDTGGSVRVPASYCGILGFRPSHG VSTSGVIPMTQSFDTVGWFA DSA+F
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAIDSAVF 180

Query: 181 KRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGG--HLIKQI 240
           KRVG+LLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAF+NSVKKLFG   HLIKQI
Sbjct: 181 KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGAADHLIKQI 240

Query: 241 SLGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSH 300
           S+G+YVEDKVPSLKHFMIE+N+GYKHSIPSLAALVRSK LLQRHEFKINHGEW+RSY+SH
Sbjct: 241 SIGSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVRSYSSH 300

Query: 301 LGPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTD 360
           LGPG SERI EYIR ATDENTDLS S+QIELR ALAAL EDFGVL IPTVPGPPPKLNTD
Sbjct: 301 LGPGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPPKLNTD 360

Query: 361 ISEMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLY 420
           ISE++DFRAKAFSLL+IA+VSG+CQ +IPLGLYNGLPVSISLLAKHGSDGFLLNVVD+LY
Sbjct: 361 ISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTLY 420

BLAST of HG10007097 vs. ExPASy TrEMBL
Match: A0A1S3BQD5 (amidase 1-like OS=Cucumis melo OX=3656 GN=LOC103492181 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 9.9e-215
Identity = 382/430 (88.84%), Postives = 400/430 (93.02%), Query Frame = 0

Query: 1   MAVQDHGAFIEKFLLQMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQ 60
           MAV D GAFIEKFLLQMSS SDQLPL+GLTFAVKDIFDI+G+VAGFGNPEWLRTH PANQ
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV- 120
           TAPAVL IL+GGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGS VAV 
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIF 180
                   GTDTGGSVRVPASYCG+LGFRPSHG VSTSGVIPMTQSFDTVGWFARDSAI 
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGQLLLQEPEVEHYKPTQVLIAEDCFK-LSSIPSERLTQAFINSVKKLFGGHLIKQIS 240
           KRVG LLLQEPEVEHYKPT+V IAEDCFK LSSI SERLTQAF++SV+KLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHL 300
           LG YVEDKVPSLKHFMIE+N+GYKHSIPSLAALVRSKRLLQRHEFKINHGEW+ SY+SHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300

Query: 301 GPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISE+ILE+IREATDEN DLSRSIQIELRE LAAL EDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYS 420
           SE +DFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLN+VD LYS
Sbjct: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420

BLAST of HG10007097 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 502.3 bits (1292), Expect = 3.8e-142
Identity = 258/417 (61.87%), Postives = 313/417 (75.06%), Query Frame = 0

Query: 5   DHGAFIEKFLLQMSSLSDQLP-LTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQTAP 64
           D GAFIEK  +  +S S   P L GLTFA+KDIFD++G V GFGNP+WLRTHS A  TAP
Sbjct: 6   DFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAP 65

Query: 65  AVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV---- 124
            V ++L+ GAT +G TIMDEMA+SINGEN HYGTP+NP A DR+PGGSSSGSAVAV    
Sbjct: 66  VVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARL 125

Query: 125 -----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIFKRV 184
                GTDTGGSVRVPASYCGI GFRPSHGAVST G+ PM QSFDTVGWFARD+A  KRV
Sbjct: 126 VDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKRV 185

Query: 185 GQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGH-LIKQISLGN 244
           G +LLQ+  +   +P+Q++IA+DCFKL S+P + L Q  + SV+K FGG+ ++K+++LG 
Sbjct: 186 GCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNLGE 245

Query: 245 YVEDKVPSLKHFMIEDN--EGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHLG 304
           Y+   VPSLKHFM  D+     +  IPSL AL  S RLLQRHEFKINHG W+ S     G
Sbjct: 246 YIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPEFG 305

Query: 305 PGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDIS 364
           PGISERI E IR  +DE  D  RS++ EL  AL+ L  + GVL IPTVPGPPP L  +++
Sbjct: 306 PGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQANVA 365

Query: 365 EMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSL 409
            +  FR++AFSLL+IA VSG CQVSIPLGL+  LPVS+SL+AK+GSDGFLL++VDSL
Sbjct: 366 ALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of HG10007097 vs. TAIR 10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III )

HSP 1 Score: 396.0 bits (1016), Expect = 3.8e-110
Identity = 200/424 (47.17%), Postives = 278/424 (65.57%), Query Frame = 0

Query: 4   QDHGAFIEKFLL----QMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPAN 63
           +D GAFI+K +L    Q +      PLTGLTFAV D+FDI GYV GFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV 123
            T+P V  +++GGATC+GKT++DE AFSI+GEN HY +P NP A  RIPGG+ SG+AVAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 ---------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAI 183
                    G DT G VRVPA YCG+LGF+ S+GA+S +G+IP++ S D+VGWFARD   
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 FKRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQIS 243
            +RVG +LLQ P      P Q+++A+DCF+L  IP +R+TQ    S +KLFG  L+K  +
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGRQLLKHQN 271

Query: 244 LGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHL 303
           L  Y E KVPSLK F           + +   L    +LLQRHEF  NHG+W+ +    +
Sbjct: 272 LETYFETKVPSLKEF-ARTKAIANTKVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPAI 331

Query: 304 GPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDI 363
            P I  ++ E   E T+E T+   +I+ E R A+ +L +D G+L IPT+P  PPKL +  
Sbjct: 332 DPVILSQVCEN-PELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSKE 391

Query: 364 SEMYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLYS 415
               D++ +A SLL+IA++SG CQV++PLG +   P+S+S + +HG D FLL+ V ++Y 
Sbjct: 392 ITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMYP 451

BLAST of HG10007097 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 349.7 bits (896), Expect = 3.2e-96
Identity = 182/428 (42.52%), Postives = 266/428 (62.15%), Query Frame = 0

Query: 4   QDHGAFIEKFLL----QMSSLSDQLPLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPAN 63
           +D GAF+++F L         + +  L+GLTF++ D FD+  Y+ GFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLAILKGGATCIGKTIMDEMAFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV 123
           +TA  V  +LK GATC+GKTIMDE+ F I GEN HYGTP NP   D +PGG SSGSAV+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 GT---------DTGGSVRVPASYCGILGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAI 183
           G          DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA D ++
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 FKRVGQLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQIS 243
             +VG  LL    V H +   ++ A+D F+LS IP ++  Q    +++ L G    K ++
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGYKTPKHVN 284

Query: 244 LGNYVEDKVPSLKHFMIEDNEGYKHSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHL 303
           +G YV   VPSL  F  E +   ++S  +L AL      +QRHEFK NH EW ++  S L
Sbjct: 285 VGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSFL 344

Query: 304 GPGISERILEYIREATDENTDLSRSIQIELREALAALFEDFGVLAIPTVPGPPPKLNTDI 363
           GP  S  ++  ++   +    L R ++ E+R  + +L ++ G+L IPTV  PPP+LNT  
Sbjct: 345 GPRFSNDVVTALKSKNESIKSLYR-VKNEMRATIQSLLKEDGILVIPTVADPPPRLNTKR 404

Query: 364 SE-MYDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNVVDSLY 418
           ++ + +F  + ++L  IA++SG CQV+IPLG +   P+S+SLL  +G D FLL+    +Y
Sbjct: 405 NKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDVY 464

BLAST of HG10007097 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 91.3 bits (225), Expect = 2.0e-18
Identity = 56/149 (37.58%), Postives = 75/149 (50.34%), Query Frame = 0

Query: 25  PLTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQTAPAVLAILKGGATCIGKTIMDEM 84
           PL G+   VKD     G  +   +   +  H      A AV  I + G   +GKT MDE 
Sbjct: 107 PLAGVLIGVKDNICTQGMPSTAASR--ILEHYRPPFDATAVKKIKELGGIVVGKTNMDEF 166

Query: 85  AFSINGENFHYGTPQNPYASDRIPGGSSSGSAVAV---------GTDTGGSVRVPASYCG 144
                 E   +    NP+   R+PGGSS GSA AV         G+DTGGSVR PAS+CG
Sbjct: 167 GMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCG 226

Query: 145 ILGFRPSHGAVSTSGVIPMTQSFDTVGWF 165
           ++G +P++G VS  G++    S D +G F
Sbjct: 227 VVGLKPTYGRVSRFGLMAYASSLDVIGCF 253

BLAST of HG10007097 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 85.9 bits (211), Expect = 8.5e-17
Identity = 95/407 (23.34%), Postives = 158/407 (38.82%), Query Frame = 0

Query: 26  LTGLTFAVKDIFDIDGYVAGFGNPEWLRTHSPANQTAPAVLAILKGGATCIGKTIMDEMA 85
           L G+   +KD  D   +    G   WL       + +  V  +   GA  +GK  M E+ 
Sbjct: 197 LDGIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 256

Query: 86  FSINGENFHYGTPQNPYASDRIPGGSSSGSA---------VAVGTDTGGSVRVPASYCGI 145
               G N +YGT +NP+   R  GGSSSGSA          A+GTD GGSVR+P++ CGI
Sbjct: 257 MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGI 316

Query: 146 LGFRPSHGAVSTSGVIPMTQSFDTVGWFARDSAIFKRVGQLLLQEPEVEHY--KPTQVLI 205
            G + ++G    +G +    + + +G  A        V   +L     + Y  KP     
Sbjct: 317 TGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSADRYNLKP----- 376

Query: 206 AEDCFK--LSSIPSERLTQAFINSVKKLFGGHLIKQISLGNYVEDKVPSLKHFMIEDNEG 265
           +  CF   LS   S  +    +    K F       IS      DK   +   ++ +N G
Sbjct: 377 SPPCFPKLLSHNGSNAIGSLRLGKYTKWFNDVSSSDIS------DKCEDILK-LLSNNHG 436

Query: 266 YK---HSIPSLAALVRSKRLLQRHEFKINHGEWLRSYNSHLGPGISERI-------LEYI 325
            K     +P L      + +   H   I     L S   +   G + ++           
Sbjct: 437 CKVVEIVVPEL------EEMRAAHVISIG-SPTLSSLTPYCEAGKNSKLSYDTRTSFAIF 496

Query: 326 REATDENTDLSRSIQIELREALAALFEDFGVLAIP----TVPGPPPKLNTDISEMYDFRA 385
           R  +  +   ++ ++  L E    +F+D  V+  P    T P  PP    +         
Sbjct: 497 RSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDALKNGETNIQVTT 556

Query: 386 KAFSLLTIAAVSGVCQVSIPLGL-YNGLPVSISLLAKHGSDGFLLNV 405
                +  A + G   +S+P+G    GLP+ + ++ +  ++  +L +
Sbjct: 557 DLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGL 583