HG10007089 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10007089
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionSeryl-tRNA synthetase
LocationChr10: 1163000 .. 1167433 (-)
RNA-Seq ExpressionHG10007089
SyntenyHG10007089
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGACATCAATTTGTTCAGGGAAGAGAAGGGAAACAATCCTGAAATCATCCGTGAGTCTCAACGCCGCCGATTTGCTGACGTTGGACTCGTTGATGAAGTCATTAAGTTTGACAAGGAATGGCGTCAACGTAATCCTCCATCCTTCTCTATCGCTTTTATTTTCCCGTTTTCTGTTGATTCTGTTTCTTACATTTTTTGGAATTAGAATTTCTTCAGGCCAGTTTGAGCTTGAGAATCTCAGGAAGGAGTTCAACAAGATCAATAAGCAAATTGCTCAGCTCAGAATAGTAACTCATCTATCTCCATTCTTAGAAGATATTTGTGTTTTCTTTTCCGCGTTATCGGATTTCATATCTCATCTTCACTGTATTGGTTGTAAGAAAATTGGTTTAGGAACTTGTGTTCATTTCTTAGTCTGCTACTGTCTGTTTGTGATTTAGAGATTTTAAGGTTTGTCTTGTGAAATTTATGGTTTTGGTTTAATTTCTGTGCTTCCGTTGTTTACTTTGAAACTTTTGGAAATGAAGAAAGGTGAGGATGCGTCCGAGAAGATTAAAGACACTGAAGAAAATAAGAAGTTGTCTACAGAGAAAGACGTTGAAGCCAAGGATGTGTTAAAAAAGTTGAACTCCAAATTGGAAATAATTGGAAACCTTGTGCATGATTCTGTTCCCATCAGCATTGATGAGGTCTGGCATCCGACATCACTGCATTTGTTCTATTCTTGGTTGCGCCTTCCCTTTTTTTAACATTGCACTTAGCAACATTTTGTAGGCTAATAATGCTGTAATCAGATCGTGGGGTGAGAAACTGGTGAAACCAAAACTAAAGAATCATGTTGATCTGGTTGAGCTTCTTGAACTTGCAGATACCAAAAGAGGTAATGAATTTTAACAATCTTATTTTAGTCATAACAATCTTATTTTAGTCAATCTTCTATGTACATACAAGATTCATTTTCTACACCTTCTGGCTTCTATCAATTTTACTGTGGCACTAAGTGTTTCAATCTTATAATTTTGCTTCACATCTGTATCAATTGAAATCCCTTTGTTTTGACATGATTCTATGTTGCATATCCTTATTTCTTCAGAGAAAATGACAAAAAGAAGAGTAACAAGTGTAGAGTCATATGCTACCTGCATTGTAAATGTTTGAGAGGTTATTAGAATATTAGCTGTACCACCTACTCTGACAAGCTTTAGTTTATGTATTTATTTTTGAGTTGTATTCCTTTTCCTTAAACTTTACGAATTATGTATAACAGTATAACACCTTTATCCCCACACTTTGTTGAATATTTTGTTTTCTGCTGTTCAACCCTCTCCCTATCTATATGAAATTTCTTCTATATTCTATCTTGGTGGGTCACCTACTTAAGAAGAAATGGAAATATTATAGTTGCAGATTATTATCTTTACTCTTCAGGACATATTAATGAAAAGGAACAACTGTACTTTCAATGGCATTGCTCTCTTTTGATAGTTTCAAAGATACAGTTCTTACATATGCTTAGTTGGTGTATATTTTACTTTGCTTACAAGTTATAGATTGAATGGGTCGAATTGTTTATTGATTAATTGGAAGGGTTTTATGTAACTTCCTTCAGCTCTGGATGTTCGCCCTATATTTCATCTCATCTAATGAAATTCCTAATTTTTCACATGTCTGTTTTGGAATTCTATATCCCCATAAAAATTTAAAATATAGATGCAGAAAGGTAAGCTTTTATGCTAATTCCTTCTTGATGCAAATATGTAGGTTCTGAAATAGCAGGAGGTAGAGGCTACTACCTAATAGGAGCTGGTATGCGTTTGAACCAAGCTTTGATAAATTATGGCCTTGACTTTTTGGAGAAAAAGGGTTACACACCCCTGCAGACTCCTTTCTTCATGAGAAAAGATATTATGGCTAGATGTGCTCAACTAGCACAATTTGATGAAGAACTTTATAAGGTCTGCATTATTACCCTCTGCTTGTTTTGTTAGAATTATTTGGAAATTTAAATCCCGATGGATTTGAATGAATTCCTACCTTATTACTTGAAATAAATAGGAACTTGATTTCAAATCTTGTTTAAAAAATTTCAGACTAAAATTGTTGGATTTCATTGGATTTTATAGTCGACTACTAATTAGGAATTTTTTTAATCAAAATTTCTAGTGAACATTTGAACTAATTTATCCAAATTCATTTCAAATCCTTCTCATTCCAAACAACACAATAATGTTCAAATCATTTTTTGGTCTTTTAATTCATATGATAATCCATCTTTATACTTCTTTTATTAAAAAAATATCTTAGTGGTTTTCTCTCTCTGGAAAGTATAGTTTTTGCTTTTATTTCAGAACAAGGGATCTCAGTCCATTAGTAAGACAATTTTTTGCTTTGTTTGGGTTGTGGAAGGCTGTTAAAAAAATGACCATGAAATAATTGATGTTCCTTAGGTCACCGGTGAAGGGGATGATAAATATTTAATTGCCACTGCTGAACAAGCACTTTGTGCATATCATCTTGAAGAATGGATTCATCCTTCGCAGTTGCCTATAAGGTTCTTGCATCTCGTTAAAAGCCTGTCTACAGCTGGCACCCTAAGAACGTGTTTTGTTTAACTCACTATCATTAATTTTCTGTACAGATATGCTGGATATTCATCTTGCTTCCGCAAAGAAGCGGGTTCCCATGGCCGAGATACTCTTGGAATTTTCCGAGTACACCAATTTGAGAAAGTAGAGCAATTCTGTATCACTAGCCCAAATGGCAATGACTCGTGGGACATGCTCGAAGAGATGATTAAAAATTCTGAGGAGTTCTACAAGACGGTAGCTTTTGTTCCCTGTATTGGTGGTTTTTATCTTCCAACTCATTTATGATTCCATGTTAGGAAACTGGTATTTTGGTTATTTTATGGAACATTTGAATGGAGCACAATAGTGATATTTTTGGAGGGGCTGAGAGATCCAAGATCTTAGGATGAGGTTTGGTCTTGTTCAGTCTTTTTCTTCTCTTTGGGCATTGGTTTTAGGGTTTTTTCTTTTTAGTGCTTAAATGATTTATTTACCTCTTATTCTTTGGATTGGGCCCTCTTTTTATTTATTTGTTTATTTTTGCATAGTTAGTTTTGGTATTTATTTGTTCTTTTTTATTAGCCTGTCTTTCTTGTATGCCTTTTTGTTTTCTTGCATTCCTTCATTTTGTTCTTAGTGGAAGCTTGGTTTTCCATTAGAAAAAAGGTTAGTCGTAATCTTTGTCTGATGTGGGATCATATGCCTTGGCAGTTGAATATCCCATACCAAGTCGTGGCTATAGTTTCTGGTGCATTAAATGATGCAGCTGCCAAGAAATACGATTTAGAAGCATGGTTTCCTGCATCTCAGACTTACAGGGAGTTAGTATCCTGTTCAAACTGCACTGATTATCAGTCCAGAAGGTTAGAGATTCGTTATGGCCAGAAAAAGGTTAGTTCTTTTTCATTACTTCTGAATTAGTGGAGGATATGTTTTGCCATTAAGATTGTTGAACTTGGCAGTGTTAACTTTTTAACAGGTTTTTAATAGGATTTATTCTTGCAACTTACTTGATGTCTATGATTCTGGCATTTTTGTAACTCCACGATGTTTTTTTCGGTTGAATAATATCTTTGGTCCTAAACTTTCAGGGTTGCCTATAGACACTTTGTATTTTATCAAGTTTTTAACTGGTATTAAACTTTCAAGCTTGGCTTATGTCTCTTTAAACCCTATATTTTTCACAAGATTCTAATATACCCATGAGTTCTGTTGGATTAAAGGAGAGATGAATGCATCTCTCCTGACAGGGTTGGGCCACCCTGTTAACACATCAAAGCTAATCACCTCTCACAGGTGGATCCAATTCAAAAAAATATACCCATGAGTTTGTAATTGTGTGTTTAATTAGTCTCCACCAATAACTCTATTAGTTTTATGAATATGCCAGACATCATCTAGACTTGATTTAGGTTTAATGCTTATGCCACACAATGTTAAAACGATGAGCCTGTCGTATTATGAACGTTTTCAAGTGCGAGGATTAGATAGACAACCTAAAATGTTTAGGGATTTATTATTAACTTTTAAAGTTTCTATGAACGACACAGCCTTGAAAACTGAAGGATACGATACAACTTTTGTTTTTTCATTCCCATTCCCATTCCCAAATGTTGAACTGCTATTTGGTTTCTGTTGTGCAACTGCAGAGCAATGAACAGACGAAACAATATGTGCACTTGCTGAATTCTACTCTCACAGCAACAGAAAGGACTCTATGTTGCATTCTTGAAAACTACCAGAAAGAGGATGGGGTTGAAATTCCTGAAGTTCTACAACCATATATGGGTGGGAAGACTTTTCTACCCTTCAAAGCAAAACCGGCGACCAGCGATGCCAAAGGGAAGAAATCAAAGGCCTAA

mRNA sequence

ATGTTGGACATCAATTTGTTCAGGGAAGAGAAGGGAAACAATCCTGAAATCATCCGTGAGTCTCAACGCCGCCGATTTGCTGACGTTGGACTCGTTGATGAAGTCATTAAGTTTGACAAGGAATGGCGTCAACGCCAGTTTGAGCTTGAGAATCTCAGGAAGGAGTTCAACAAGATCAATAAGCAAATTGCTCAGCTCAGAATAGTAACTCATCTATCTCCATTCTTAGAAGATATTTGTGTTTTCTTTTCCGCGTTATCGGATTTCATATCTCATCTTCACTGTATTGGTTGTTTGTCTTGTGAAATTTATGGTTTTGGTTTAATTTCTGTGCTTCCGTTGTTTACTTTGAAACTTTTGGAAATGAAGAAAGGTGAGGATGCGTCCGAGAAGATTAAAGACACTGAAGAAAATAAGAAGTTGTCTACAGAGAAAGACGTTGAAGCCAAGGATGTGTTAAAAAAGTTGAACTCCAAATTGGAAATAATTGGAAACCTTGTGCATGATTCTGTTCCCATCAGCATTGATGAGGCTAATAATGCTGTAATCAGATCGTGGGGTGAGAAACTGGTGAAACCAAAACTAAAGAATCATGTTGATCTGGTTGAGCTTCTTGAACTTGCAGATACCAAAAGAGGTTCTGAAATAGCAGGAGGTAGAGGCTACTACCTAATAGGAGCTGGTATGCGTTTGAACCAAGCTTTGATAAATTATGGCCTTGACTTTTTGGAGAAAAAGGGTTACACACCCCTGCAGACTCCTTTCTTCATGAGAAAAGATATTATGGCTAGATGTGCTCAACTAGCACAATTTGATGAAGAACTTTATAAGGTCACCGGTGAAGGGGATGATAAATATTTAATTGCCACTGCTGAACAAGCACTTTGTGCATATCATCTTGAAGAATGGATTCATCCTTCGCAGTTGCCTATAAGATATGCTGGATATTCATCTTGCTTCCGCAAAGAAGCGGGTTCCCATGGCCGAGATACTCTTGGAATTTTCCGAGTACACCAATTTGAGAAAGTAGAGCAATTCTGTATCACTAGCCCAAATGGCAATGACTCGTGGGACATGCTCGAAGAGATGATTAAAAATTCTGAGGAGTTCTACAAGACGTTGAATATCCCATACCAAGTCGTGGCTATAGTTTCTGGTGCATTAAATGATGCAGCTGCCAAGAAATACGATTTAGAAGCATGGTTTCCTGCATCTCAGACTTACAGGGAGTTAGTATCCTGTTCAAACTGCACTGATTATCAGTCCAGAAGGTTAGAGATTCGTTATGGCCAGAAAAAGAGCAATGAACAGACGAAACAATATGTGCACTTGCTGAATTCTACTCTCACAGCAACAGAAAGGACTCTATGTTGCATTCTTGAAAACTACCAGAAAGAGGATGGGGTTGAAATTCCTGAAGTTCTACAACCATATATGGGTGGGAAGACTTTTCTACCCTTCAAAGCAAAACCGGCGACCAGCGATGCCAAAGGGAAGAAATCAAAGGCCTAA

Coding sequence (CDS)

ATGTTGGACATCAATTTGTTCAGGGAAGAGAAGGGAAACAATCCTGAAATCATCCGTGAGTCTCAACGCCGCCGATTTGCTGACGTTGGACTCGTTGATGAAGTCATTAAGTTTGACAAGGAATGGCGTCAACGCCAGTTTGAGCTTGAGAATCTCAGGAAGGAGTTCAACAAGATCAATAAGCAAATTGCTCAGCTCAGAATAGTAACTCATCTATCTCCATTCTTAGAAGATATTTGTGTTTTCTTTTCCGCGTTATCGGATTTCATATCTCATCTTCACTGTATTGGTTGTTTGTCTTGTGAAATTTATGGTTTTGGTTTAATTTCTGTGCTTCCGTTGTTTACTTTGAAACTTTTGGAAATGAAGAAAGGTGAGGATGCGTCCGAGAAGATTAAAGACACTGAAGAAAATAAGAAGTTGTCTACAGAGAAAGACGTTGAAGCCAAGGATGTGTTAAAAAAGTTGAACTCCAAATTGGAAATAATTGGAAACCTTGTGCATGATTCTGTTCCCATCAGCATTGATGAGGCTAATAATGCTGTAATCAGATCGTGGGGTGAGAAACTGGTGAAACCAAAACTAAAGAATCATGTTGATCTGGTTGAGCTTCTTGAACTTGCAGATACCAAAAGAGGTTCTGAAATAGCAGGAGGTAGAGGCTACTACCTAATAGGAGCTGGTATGCGTTTGAACCAAGCTTTGATAAATTATGGCCTTGACTTTTTGGAGAAAAAGGGTTACACACCCCTGCAGACTCCTTTCTTCATGAGAAAAGATATTATGGCTAGATGTGCTCAACTAGCACAATTTGATGAAGAACTTTATAAGGTCACCGGTGAAGGGGATGATAAATATTTAATTGCCACTGCTGAACAAGCACTTTGTGCATATCATCTTGAAGAATGGATTCATCCTTCGCAGTTGCCTATAAGATATGCTGGATATTCATCTTGCTTCCGCAAAGAAGCGGGTTCCCATGGCCGAGATACTCTTGGAATTTTCCGAGTACACCAATTTGAGAAAGTAGAGCAATTCTGTATCACTAGCCCAAATGGCAATGACTCGTGGGACATGCTCGAAGAGATGATTAAAAATTCTGAGGAGTTCTACAAGACGTTGAATATCCCATACCAAGTCGTGGCTATAGTTTCTGGTGCATTAAATGATGCAGCTGCCAAGAAATACGATTTAGAAGCATGGTTTCCTGCATCTCAGACTTACAGGGAGTTAGTATCCTGTTCAAACTGCACTGATTATCAGTCCAGAAGGTTAGAGATTCGTTATGGCCAGAAAAAGAGCAATGAACAGACGAAACAATATGTGCACTTGCTGAATTCTACTCTCACAGCAACAGAAAGGACTCTATGTTGCATTCTTGAAAACTACCAGAAAGAGGATGGGGTTGAAATTCCTGAAGTTCTACAACCATATATGGGTGGGAAGACTTTTCTACCCTTCAAAGCAAAACCGGCGACCAGCGATGCCAAAGGGAAGAAATCAAAGGCCTAA

Protein sequence

MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKINKQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLLEMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANNAVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGLDFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHLEEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDMLEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMGGKTFLPFKAKPATSDAKGKKSKA
Homology
BLAST of HG10007089 vs. NCBI nr
Match: XP_038892840.1 (serine--tRNA ligase isoform X2 [Benincasa hispida] >XP_038892841.1 serine--tRNA ligase isoform X2 [Benincasa hispida])

HSP 1 Score: 827.8 bits (2137), Expect = 5.1e-236
Identity = 421/503 (83.70%), Postives = 435/503 (86.48%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V LVDEVIK DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASEKIKDTEENKKLS EKDVEAKDVLKKLNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASEKIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
            VIRSWGEKLVKPKLKNHVDLVELLE+ADTK+GSEIAGGRGYYL G G+RLNQALINYGL
Sbjct: 181 VVIRSWGEKLVKPKLKNHVDLVELLEIADTKKGSEIAGGRGYYLKGVGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLE++GYT LQTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQ LC+YHL
Sbjct: 241 DFLEQRGYTALQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCSYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDM 
Sbjct: 301 EDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSE+FYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PEVLQPY+G
Sbjct: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVLQPYLG 448

Query: 481 GKTFLPFKAKPATSDAKGKKSKA 504
           GKTFLPFKAKP  SDAKGKKSKA
Sbjct: 481 GKTFLPFKAKPTASDAKGKKSKA 448

BLAST of HG10007089 vs. NCBI nr
Match: XP_022145958.1 (serine--tRNA ligase [Momordica charantia] >XP_022145959.1 serine--tRNA ligase [Momordica charantia])

HSP 1 Score: 825.9 bits (2132), Expect = 1.9e-235
Identity = 421/503 (83.70%), Postives = 437/503 (86.88%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V LVDEVIK DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASEKIKDTEENKKLS EKDVEAKDVLK+LNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
           A+IRSWGEKLVKPKLKNHVDLVELL +ADTK+GSEIAGGRG+YL G G+RLNQALINYGL
Sbjct: 181 AIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLEK+GYT LQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQ LCAYHL
Sbjct: 241 DFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCAYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFC+TSPNGNDSWDM 
Sbjct: 301 EDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSE+FYKTLN+PYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQP+MG
Sbjct: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPFMG 448

Query: 481 GKTFLPFKAKPATSDAKGKKSKA 504
           GKTFLPFKAKP+TS+AKGKKSKA
Sbjct: 481 GKTFLPFKAKPSTSEAKGKKSKA 448

BLAST of HG10007089 vs. NCBI nr
Match: XP_008464736.1 (PREDICTED: serine--tRNA ligase, cytoplasmic [Cucumis melo] >XP_008464737.1 PREDICTED: serine--tRNA ligase, cytoplasmic [Cucumis melo] >KAA0065494.1 serine--tRNA ligase [Cucumis melo var. makuwa] >TYK08734.1 serine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 823.5 bits (2126), Expect = 9.6e-235
Identity = 418/503 (83.10%), Postives = 434/503 (86.28%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V LVDEVIK DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASE+IKDTEENK+LS EKDVEAKDVLKKLNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASERIKDTEENKRLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
            VIRSWGEKLVKPKLKNHVDLVELLE+ADTKRGSEIAGGRGYYL GAG+RLNQALINYGL
Sbjct: 181 VVIRSWGEKLVKPKLKNHVDLVELLEIADTKRGSEIAGGRGYYLKGAGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLEK+GYT LQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQ LC+YHL
Sbjct: 241 DFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCSYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDM 
Sbjct: 301 EDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSE+FYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PEVL+PY+G
Sbjct: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVLRPYLG 448

Query: 481 GKTFLPFKAKPATSDAKGKKSKA 504
           GK FLPFK KP  +D+KGKKSKA
Sbjct: 481 GKAFLPFKTKPTVNDSKGKKSKA 448

BLAST of HG10007089 vs. NCBI nr
Match: XP_011654444.1 (serine--tRNA ligase [Cucumis sativus] >XP_031741845.1 serine--tRNA ligase [Cucumis sativus] >KGN49564.1 hypothetical protein Csa_018487 [Cucumis sativus])

HSP 1 Score: 822.8 bits (2124), Expect = 1.6e-234
Identity = 420/503 (83.50%), Postives = 433/503 (86.08%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V LVDEVIK DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASE+IKDTEENKKLS EKDVEAKDVLKKLNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
            VIRSWGEKLVKPKLKNHVDLVELLE+AD+KRGSEIAGGRGYYL GAG+RLNQALINYGL
Sbjct: 181 VVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLEK+GYT LQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQ LC+YHL
Sbjct: 241 DFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCSYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPN NDSWDM 
Sbjct: 301 EDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PEVL+PY+G
Sbjct: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVLRPYLG 448

Query: 481 GKTFLPFKAKPATSDAKGKKSKA 504
           GK FLPFK KP  SD+KGKKSKA
Sbjct: 481 GKAFLPFKTKPTGSDSKGKKSKA 448

BLAST of HG10007089 vs. NCBI nr
Match: XP_038892839.1 (serine--tRNA ligase isoform X1 [Benincasa hispida])

HSP 1 Score: 819.7 bits (2116), Expect = 1.4e-233
Identity = 416/502 (82.87%), Postives = 432/502 (86.06%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V LVDEVIK DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASEKIKDTEENKKLS EKDVEAKDVLKKLNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASEKIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
            VIRSWGEKLVKPKLKNHVDLVELLE+ADTK+GSEIAGGRGYYL G G+RLNQALINYGL
Sbjct: 181 VVIRSWGEKLVKPKLKNHVDLVELLEIADTKKGSEIAGGRGYYLKGVGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLE++GYT LQTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQ LC+YHL
Sbjct: 241 DFLEQRGYTALQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCSYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDM 
Sbjct: 301 EDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSE+FYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PEVLQPY+G
Sbjct: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVLQPYLG 447

Query: 481 GKTFLPFKAKPATSDAKGKKSK 503
           GKTFLPFKAKP  SDAKG  ++
Sbjct: 481 GKTFLPFKAKPTASDAKGASNR 447

BLAST of HG10007089 vs. ExPASy Swiss-Prot
Match: Q39230 (Serine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g27470 PE=1 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 3.5e-203
Identity = 352/506 (69.57%), Postives = 406/506 (80.24%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA V +VDE+IK DKEWRQRQFE+++ RKEFNK+N
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVEIVDEIIKLDKEWRQRQFEVDSFRKEFNKLN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQ+AQL+I                                                    
Sbjct: 61  KQVAQLKI---------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
              K EDASE I+ TE+NK+ ST K+ E ++    L +KLE +GNLVHDSVP+  DEANN
Sbjct: 121 ---KKEDASEIIQQTEKNKQDSTAKEAEVREAYAALKAKLEQVGNLVHDSVPVDKDEANN 180

Query: 181 AVIRSWGEKLVKP---KLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALIN 240
            VI+ WGEK       KLKNHVDLVELL +ADTKRG+EIAG RG++L G G+ LNQALIN
Sbjct: 181 LVIKLWGEKRFSTPGLKLKNHVDLVELLGIADTKRGAEIAGARGFFLKGDGLMLNQALIN 240

Query: 241 YGLDFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCA 300
           +GL FL+K+G+T LQ PFFMRKD+MA+CAQLAQFDEELYKVTGEGDDKYLIATAEQ LCA
Sbjct: 241 FGLTFLKKRGFTGLQPPFFMRKDVMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCA 300

Query: 301 YHLEEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSW 360
           YH++EWIHP++LP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEK+EQFCIT PN N SW
Sbjct: 301 YHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKIEQFCITGPNENASW 360

Query: 361 DMLEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNC 420
           +ML+EM+KNSE+FY+ L +PYQ+V+IVSGALNDAAAKKYDLEAWFP+S+T+RELVSCSNC
Sbjct: 361 EMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAKKYDLEAWFPSSETFRELVSCSNC 420

Query: 421 TDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQP 480
           TDYQ+RRLEIRYGQKKSNEQTKQYVH+LNSTLTATERT+CCILENYQ+EDGV+IPEVLQP
Sbjct: 421 TDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATERTICCILENYQREDGVDIPEVLQP 451

Query: 481 YMGGKTFLPFKAKPATSDAKGKKSKA 504
           +MGG+TFLPFKAKP  +D KGKKSKA
Sbjct: 481 FMGGETFLPFKAKPVVADTKGKKSKA 451

BLAST of HG10007089 vs. ExPASy Swiss-Prot
Match: O81983 (Serine--tRNA ligase OS=Helianthus annuus OX=4232 PE=2 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 4.5e-195
Identity = 341/490 (69.59%), Postives = 385/490 (78.57%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKG NPEI+RESQRRR A V +VDE+IK DKEWRQRQFELE LRK+FN+IN
Sbjct: 1   MLDINLFREEKGGNPEIVRESQRRRGASVEIVDEIIKLDKEWRQRQFELEQLRKDFNRIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           K++A+LRI                                                    
Sbjct: 61  KEVAKLRI---------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               G DAS  IK+TEENK  + +K  E ++    L SKLE +GNLVHDSVP+S DEA+N
Sbjct: 121 ---SGGDASSLIKNTEENKDSTAKKQAEVQEARTALYSKLETVGNLVHDSVPVSNDEADN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
           AV+R+WGE+  +  LKNHV+LVELL +AD K+G+ +AGGRGYYL G G+RLNQALIN+GL
Sbjct: 181 AVVRTWGERREETNLKNHVELVELLGIADLKKGANVAGGRGYYLKGDGVRLNQALINFGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLEK+ YT LQTPFFMRKDIM +CAQLAQFDEELYKVTGEGDDKYLIATAEQ LCAYH+
Sbjct: 241 DFLEKRKYTALQTPFFMRKDIMGKCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCAYHI 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           ++WIHP++LP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFC+T P+GNDSW+M 
Sbjct: 301 DDWIHPNELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCLTKPSGNDSWEMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSEEFY+ L +PYQVV+IVSGALNDAAAKKYDLEAWFPAS+TYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEEFYQMLKLPYQVVSIVSGALNDAAAKKYDLEAWFPASKTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSR+LEIR GQKK NEQ KQY HLLNSTLTATERTLCCILENYQ E GV IPEVLQP+MG
Sbjct: 421 QSRKLEIRCGQKKGNEQAKQYCHLLNSTLTATERTLCCILENYQTEKGVAIPEVLQPFMG 435

Query: 481 GKTFLPFKAK 491
           GKTFL F  K
Sbjct: 481 GKTFLDFLKK 435

BLAST of HG10007089 vs. ExPASy Swiss-Prot
Match: O14018 (Serine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC29A4.15 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 2.1e-136
Identity = 266/511 (52.05%), Postives = 336/511 (65.75%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLF+ EKG NPEIIRESQR+R ADVG+VD+VI+  KEW   +FEL+N  K  N++ 
Sbjct: 1   MLDINLFQVEKGGNPEIIRESQRKRGADVGVVDKVIEMYKEWVSLRFELDNTNKSINRVQ 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           K+I                                                        L
Sbjct: 61  KEIG-------------------------------------------------------L 120

Query: 121 EMKKGEDASEKIKD----TEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISID 180
           +MK  EDASE +++    TE  K L  ++  + K++L  ++S    IGN+VHDSVP+S+D
Sbjct: 121 KMKAKEDASELLEEKNSLTERKKNLIEQETAKNKEMLNVVSS----IGNIVHDSVPVSMD 180

Query: 181 EANNAVIRSWGEKLVKPKLKN---HVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQ 240
           E NN +IR W  + V  + KN   H +++  L+  D +RG +++G RGY+L   G+  N 
Sbjct: 181 EDNNEIIRKWAPEGVTVEKKNCLSHHEVLTRLDGYDPERGVKVSGHRGYFLRQYGVFFNL 240

Query: 241 ALINYGLDFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQ 300
           ALI YGLDFLEK+GY  LQ P  + KD+MA+ AQL QFDEELYKV    +++YLIAT+EQ
Sbjct: 241 ALIQYGLDFLEKRGYIALQAPTMLNKDVMAKTAQLEQFDEELYKVIDGDEERYLIATSEQ 300

Query: 301 ALCAYHLEEWIH-PS-QLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSP 360
            + AYH  EW   PS QLP++YAGYS+C+R+EAGSHGRD  GIFRVH FEK+EQF +T P
Sbjct: 301 PISAYHSGEWFEKPSEQLPLKYAGYSTCYRREAGSHGRDAWGIFRVHAFEKIEQFVLTDP 360

Query: 361 NGNDSWDMLEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYREL 420
               SW+   EMI ++E+FYK+L +PY++VAIVSGALN+AAAKKYDLEAWFP    Y+EL
Sbjct: 361 --EKSWEAFTEMINHAEDFYKSLELPYRIVAIVSGALNNAAAKKYDLEAWFPFQGEYKEL 420

Query: 421 VSCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEI 480
           VSCSNCTDYQSR LEIR G KK  ++ K+YVH LNSTL ATER LCCILENYQ  DGV +
Sbjct: 421 VSCSNCTDYQSRNLEIRCGVKKMGDREKKYVHCLNSTLCATERALCCILENYQTPDGVNV 450

Query: 481 PEVLQPYMGGKTFLPF-KAKPATSDAKGKKS 502
           P+VLQPYMGGKTFLPF K  P  S +K  K+
Sbjct: 481 PKVLQPYMGGKTFLPFTKELPKNSTSKKGKN 450

BLAST of HG10007089 vs. ExPASy Swiss-Prot
Match: Q7KWQ2 (Serine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=serS PE=1 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 9.9e-134
Identity = 250/491 (50.92%), Postives = 320/491 (65.17%), Query Frame = 0

Query: 2   LDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKINK 61
           LDI LFR +KG NP++IR+SQ+ R+A+V  V EVI  DK  ++ ++ L+N   E+ K+NK
Sbjct: 3   LDIVLFRADKGGNPDLIRQSQKVRYANVDAVQEVIDLDKVVKETKYRLDNTNAEYAKLNK 62

Query: 62  QIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLLE 121
            +A                                                       ++
Sbjct: 63  SVA-------------------------------------------------------MK 122

Query: 122 MKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANNA 181
            K GE A E I   EE  +   +   E  +V   L  KL  IGN+VH+SVPI  +E NN 
Sbjct: 123 KKAGESADEIIAQAEELNQSIIKLKAEVSEVELLLRKKLRPIGNIVHESVPIDNNEDNNQ 182

Query: 182 VIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGLD 241
           +I++WGE      L +H +L+E+++  D +RG+ ++G R Y+L G G+ LNQA+IN+ L 
Sbjct: 183 IIKTWGECKTSEGLLHHHELLEMIDGYDPERGTLVSGHRCYFLKGIGVLLNQAIINFALM 242

Query: 242 FLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHLE 301
            + K+G  PLQTPFFM KD+MA+ AQL QFD+ELYKVTG+ ++KYLIAT+EQ + A+H +
Sbjct: 243 HMTKRGSVPLQTPFFMNKDVMAKTAQLEQFDDELYKVTGDNEEKYLIATSEQPISAFHQD 302

Query: 302 EWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDMLE 361
           EWI    LP +Y GYS+CFRKEAGSHGRDT GIFRVHQFEK+EQFCIT P    SWDM+E
Sbjct: 303 EWIEEKDLPKKYVGYSTCFRKEAGSHGRDTWGIFRVHQFEKIEQFCITEP--EKSWDMME 362

Query: 362 EMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDYQ 421
           EMI NSE+FY+ L IPY+VV IVSGALN+AA+KKYDLE WFP    YRELVSCSNCTDYQ
Sbjct: 363 EMINNSEQFYQELGIPYRVVNIVSGALNNAASKKYDLEGWFPGYNQYRELVSCSNCTDYQ 422

Query: 422 SRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMGG 481
           SR LEIR G KK  +Q K+YVH+LNSTL AT R +CCILENYQ E G+ +P  L+PY+ G
Sbjct: 423 SRDLEIRCGMKKQGQQQKKYVHMLNSTLAATTRVICCILENYQTEGGITVPVPLRPYL-G 435

Query: 482 KTFLPF-KAKP 492
           K F+PF KA P
Sbjct: 483 KDFIPFVKAAP 435

BLAST of HG10007089 vs. ExPASy Swiss-Prot
Match: Q18678 (Probable serine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=sars-1 PE=3 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.7e-125
Identity = 247/512 (48.24%), Postives = 335/512 (65.43%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           +LDI++FR EKG NPEIIR+SQ+ RF D  LVDEVI+ D++WR+ +F  + L ++ N I+
Sbjct: 2   VLDIDMFRTEKGGNPEIIRKSQQDRFKDPKLVDEVIELDEKWRKERFVADQLNRQKNAIS 61

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           K I + ++        +D     S   D ++ L               + +  L  L ++
Sbjct: 62  KAIGE-KMKKKEPQGTDD-----SVADDIVARL-------------AELKIDELSQLTVV 121

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
           ++K       K++   + K + T   V A +  +  + KL  IGNL+H SV +S DEANN
Sbjct: 122 QLK-------KLRVLVDEKSVQTAAAVIANENAR--HEKLIQIGNLIHSSVVVSKDEANN 181

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
            + R++G+   K K  +HVDLV +++  D +RG+ +AGGRGY+L G  + L QA+I   L
Sbjct: 182 KIERTFGDLSTKKKY-SHVDLVVMVDGFDGERGTVVAGGRGYFLKGPLVFLEQAIIQLAL 241

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEG---------DDKYLIATA 300
             L  KGY PL TPFFMRK++M   AQL+QFD+ELYKV+ +G         D+KYLIAT+
Sbjct: 242 QRLNVKGYVPLYTPFFMRKEVMQEVAQLSQFDDELYKVSSKGSEIAGDTSVDEKYLIATS 301

Query: 301 EQALCAYHLEEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSP 360
           EQ + AYH  EWI  ++LPI+YAG S+CFR+E GSHGRDT GIFRVHQFEK+EQF + SP
Sbjct: 302 EQPIAAYHRNEWIKETELPIKYAGVSTCFRQEVGSHGRDTRGIFRVHQFEKIEQFVLCSP 361

Query: 361 NGNDSWDMLEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYREL 420
           N N+SW + +EMI N+E +Y+ L IPYQVV IVSG LN+AAAKK+DLEAWFP S  YREL
Sbjct: 362 NDNESWTLFDEMIGNAESYYQELQIPYQVVNIVSGELNNAAAKKFDLEAWFPGSGAYREL 421

Query: 421 VSCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEI 480
           VSCSNC DYQSRRL++RYGQ K       +VH+LN+T+ AT R +C ILEN Q E+G+ +
Sbjct: 422 VSCSNCLDYQSRRLKVRYGQTKKLSGEVPFVHMLNATMCATTRVICAILENNQTEEGINV 481

Query: 481 PEVLQPYM--GGKTFLPFKAKPATSDAKGKKS 502
           P  +Q +M    +TF+PF  KPA  D   KK+
Sbjct: 482 PTAIQQWMPENYRTFIPF-VKPAPIDEDAKKA 483

BLAST of HG10007089 vs. ExPASy TrEMBL
Match: A0A6J1CWS6 (Seryl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015292 PE=4 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 9.4e-236
Identity = 421/503 (83.70%), Postives = 437/503 (86.88%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V LVDEVIK DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASEKIKDTEENKKLS EKDVEAKDVLK+LNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
           A+IRSWGEKLVKPKLKNHVDLVELL +ADTK+GSEIAGGRG+YL G G+RLNQALINYGL
Sbjct: 181 AIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLEK+GYT LQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQ LCAYHL
Sbjct: 241 DFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCAYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFC+TSPNGNDSWDM 
Sbjct: 301 EDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSE+FYKTLN+PYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQP+MG
Sbjct: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPFMG 448

Query: 481 GKTFLPFKAKPATSDAKGKKSKA 504
           GKTFLPFKAKP+TS+AKGKKSKA
Sbjct: 481 GKTFLPFKAKPSTSEAKGKKSKA 448

BLAST of HG10007089 vs. ExPASy TrEMBL
Match: A0A1S3CM54 (Seryl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103502550 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 4.6e-235
Identity = 418/503 (83.10%), Postives = 434/503 (86.28%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V LVDEVIK DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASE+IKDTEENK+LS EKDVEAKDVLKKLNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASERIKDTEENKRLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
            VIRSWGEKLVKPKLKNHVDLVELLE+ADTKRGSEIAGGRGYYL GAG+RLNQALINYGL
Sbjct: 181 VVIRSWGEKLVKPKLKNHVDLVELLEIADTKRGSEIAGGRGYYLKGAGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLEK+GYT LQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQ LC+YHL
Sbjct: 241 DFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCSYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDM 
Sbjct: 301 EDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSE+FYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PEVL+PY+G
Sbjct: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVLRPYLG 448

Query: 481 GKTFLPFKAKPATSDAKGKKSKA 504
           GK FLPFK KP  +D+KGKKSKA
Sbjct: 481 GKAFLPFKTKPTVNDSKGKKSKA 448

BLAST of HG10007089 vs. ExPASy TrEMBL
Match: A0A5D3CBX2 (Seryl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold76G00430 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 4.6e-235
Identity = 418/503 (83.10%), Postives = 434/503 (86.28%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V LVDEVIK DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASE+IKDTEENK+LS EKDVEAKDVLKKLNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASERIKDTEENKRLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
            VIRSWGEKLVKPKLKNHVDLVELLE+ADTKRGSEIAGGRGYYL GAG+RLNQALINYGL
Sbjct: 181 VVIRSWGEKLVKPKLKNHVDLVELLEIADTKRGSEIAGGRGYYLKGAGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLEK+GYT LQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQ LC+YHL
Sbjct: 241 DFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCSYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDM 
Sbjct: 301 EDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSE+FYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PEVL+PY+G
Sbjct: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVLRPYLG 448

Query: 481 GKTFLPFKAKPATSDAKGKKSKA 504
           GK FLPFK KP  +D+KGKKSKA
Sbjct: 481 GKAFLPFKTKPTVNDSKGKKSKA 448

BLAST of HG10007089 vs. ExPASy TrEMBL
Match: A0A0A0KL58 (Seryl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_5G002590 PE=4 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.9e-235
Identity = 420/503 (83.50%), Postives = 433/503 (86.08%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V LVDEVIK DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASE+IKDTEENKKLS EKDVEAKDVLKKLNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
            VIRSWGEKLVKPKLKNHVDLVELLE+AD+KRGSEIAGGRGYYL GAG+RLNQALINYGL
Sbjct: 181 VVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLEK+GYT LQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQ LC+YHL
Sbjct: 241 DFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCSYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPN NDSWDM 
Sbjct: 301 EDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PEVL+PY+G
Sbjct: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVLRPYLG 448

Query: 481 GKTFLPFKAKPATSDAKGKKSKA 504
           GK FLPFK KP  SD+KGKKSKA
Sbjct: 481 GKAFLPFKTKPTGSDSKGKKSKA 448

BLAST of HG10007089 vs. ExPASy TrEMBL
Match: A0A6J1EKK7 (Seryl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433427 PE=4 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 3.3e-233
Identity = 413/503 (82.11%), Postives = 434/503 (86.28%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA V LVDEVI+ DKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQIAQLRI                                                    
Sbjct: 61  KQIAQLRIA--------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
               GEDASEKIKDTEENKKLS EKDVEAKDVLK+LNSKLEIIGNLVHDSVP+S DEANN
Sbjct: 121 ----GEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSNDEANN 180

Query: 181 AVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALINYGL 240
           AVIRSWGEKLVKPKLKNHVDLVELL +AD+K+GSEIAGGRGYYL G G+RLNQALINYGL
Sbjct: 181 AVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQALINYGL 240

Query: 241 DFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCAYHL 300
           DFLEK+GYT +QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQ +C+YHL
Sbjct: 241 DFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPICSYHL 300

Query: 301 EEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSWDML 360
           E+WIHPSQLPIRYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGNDSWDM 
Sbjct: 301 EDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGNDSWDMH 360

Query: 361 EEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420
           EEMIKNSE+FYKTLN+PYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY
Sbjct: 361 EEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDY 420

Query: 421 QSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPYMG 480
           QSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVEIPEVLQP+MG
Sbjct: 421 QSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVLQPFMG 448

Query: 481 GKTFLPFKAKPATSDAKGKKSKA 504
           GKTF+PFKAKP TSDAKGKKSKA
Sbjct: 481 GKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of HG10007089 vs. TAIR 10
Match: AT5G27470.1 (seryl-tRNA synthetase / serine--tRNA ligase )

HSP 1 Score: 709.1 bits (1829), Expect = 2.5e-204
Identity = 352/506 (69.57%), Postives = 406/506 (80.24%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFADVGLVDEVIKFDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA V +VDE+IK DKEWRQRQFE+++ RKEFNK+N
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVEIVDEIIKLDKEWRQRQFEVDSFRKEFNKLN 60

Query: 61  KQIAQLRIVTHLSPFLEDICVFFSALSDFISHLHCIGCLSCEIYGFGLISVLPLFTLKLL 120
           KQ+AQL+I                                                    
Sbjct: 61  KQVAQLKI---------------------------------------------------- 120

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKLEIIGNLVHDSVPISIDEANN 180
              K EDASE I+ TE+NK+ ST K+ E ++    L +KLE +GNLVHDSVP+  DEANN
Sbjct: 121 ---KKEDASEIIQQTEKNKQDSTAKEAEVREAYAALKAKLEQVGNLVHDSVPVDKDEANN 180

Query: 181 AVIRSWGEKLVKP---KLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALIN 240
            VI+ WGEK       KLKNHVDLVELL +ADTKRG+EIAG RG++L G G+ LNQALIN
Sbjct: 181 LVIKLWGEKRFSTPGLKLKNHVDLVELLGIADTKRGAEIAGARGFFLKGDGLMLNQALIN 240

Query: 241 YGLDFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQALCA 300
           +GL FL+K+G+T LQ PFFMRKD+MA+CAQLAQFDEELYKVTGEGDDKYLIATAEQ LCA
Sbjct: 241 FGLTFLKKRGFTGLQPPFFMRKDVMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCA 300

Query: 301 YHLEEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGNDSW 360
           YH++EWIHP++LP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEK+EQFCIT PN N SW
Sbjct: 301 YHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKIEQFCITGPNENASW 360

Query: 361 DMLEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNC 420
           +ML+EM+KNSE+FY+ L +PYQ+V+IVSGALNDAAAKKYDLEAWFP+S+T+RELVSCSNC
Sbjct: 361 EMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAKKYDLEAWFPSSETFRELVSCSNC 420

Query: 421 TDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQP 480
           TDYQ+RRLEIRYGQKKSNEQTKQYVH+LNSTLTATERT+CCILENYQ+EDGV+IPEVLQP
Sbjct: 421 TDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATERTICCILENYQREDGVDIPEVLQP 451

Query: 481 YMGGKTFLPFKAKPATSDAKGKKSKA 504
           +MGG+TFLPFKAKP  +D KGKKSKA
Sbjct: 481 FMGGETFLPFKAKPVVADTKGKKSKA 451

BLAST of HG10007089 vs. TAIR 10
Match: AT1G11870.2 (Seryl-tRNA synthetase )

HSP 1 Score: 229.6 bits (584), Expect = 5.7e-60
Identity = 140/386 (36.27%), Postives = 218/386 (56.48%), Query Frame = 0

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKL----EIIGNLVHDSVPISID 180
           +MK   + SE+ +  EE K L  E  V  ++ L KL  +L    + I N+ H  VP+  +
Sbjct: 134 KMKGKLEPSERERLVEEGKNLK-ESLVTLEEDLVKLKDELQHVAQSIPNMTHPDVPVGGE 193

Query: 181 EANNAVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALI 240
           +++         +     +K+H+ L + L+L D    +E++G + +YL    + L  AL+
Sbjct: 194 DSSAIRQEVGSPREFSFPIKDHLQLGKDLDLIDFDSAAEVSGSKFFYLKNEAVLLEMALL 253

Query: 241 NYGLDFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDE-ELYKVTGEGDDKYLIATAEQAL 300
           N+ L  + KKGYTPL TP  +R  I+ +C    + D  ++Y +  +G D+ LI TAE  +
Sbjct: 254 NWTLSQVMKKGYTPLTTPEIVRSSIVEKCGFQPRGDNTQVYSI--DGTDQCLIGTAEIPV 313

Query: 301 CAYHLEEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 360
              H++  +  S LP++Y  +S CFR EAG+ G  T G++RVHQF K E F I  P  ++
Sbjct: 314 GGIHMDSILLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQPEDSE 373

Query: 361 SWDMLEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 420
           S+   +E+I+  E+ + +L + ++ + + +  L   A +K+D+EAW P    + E+ S S
Sbjct: 374 SFH--QELIQIEEDLFTSLGLHFKTLDMATADLGAPAYRKFDIEAWMPGLGRFGEISSAS 433

Query: 421 NCTDYQSRRLEIRY--------GQK--KSNEQTKQYVHLLNSTLTATERTLCCILENYQK 480
           NCTDYQSRRL IRY        G K  K+N    ++VH LN+T  A  R + C+LENYQ+
Sbjct: 434 NCTDYQSRRLGIRYRPSEPPQTGPKKGKANLPATKFVHTLNATACAVPRMMVCLLENYQQ 493

Query: 481 EDG-VEIPEVLQPYMGGKTFLPFKAK 491
           EDG V IPE L+P+MGG   +  K K
Sbjct: 494 EDGSVVIPEPLRPFMGGIELIKPKHK 514

BLAST of HG10007089 vs. TAIR 10
Match: AT1G11870.1 (Seryl-tRNA synthetase )

HSP 1 Score: 229.6 bits (584), Expect = 5.7e-60
Identity = 140/386 (36.27%), Postives = 218/386 (56.48%), Query Frame = 0

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKL----EIIGNLVHDSVPISID 180
           +MK   + SE+ +  EE K L  E  V  ++ L KL  +L    + I N+ H  VP+  +
Sbjct: 132 KMKGKLEPSERERLVEEGKNLK-ESLVTLEEDLVKLKDELQHVAQSIPNMTHPDVPVGGE 191

Query: 181 EANNAVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALI 240
           +++         +     +K+H+ L + L+L D    +E++G + +YL    + L  AL+
Sbjct: 192 DSSAIRQEVGSPREFSFPIKDHLQLGKDLDLIDFDSAAEVSGSKFFYLKNEAVLLEMALL 251

Query: 241 NYGLDFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDE-ELYKVTGEGDDKYLIATAEQAL 300
           N+ L  + KKGYTPL TP  +R  I+ +C    + D  ++Y +  +G D+ LI TAE  +
Sbjct: 252 NWTLSQVMKKGYTPLTTPEIVRSSIVEKCGFQPRGDNTQVYSI--DGTDQCLIGTAEIPV 311

Query: 301 CAYHLEEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 360
              H++  +  S LP++Y  +S CFR EAG+ G  T G++RVHQF K E F I  P  ++
Sbjct: 312 GGIHMDSILLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQPEDSE 371

Query: 361 SWDMLEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 420
           S+   +E+I+  E+ + +L + ++ + + +  L   A +K+D+EAW P    + E+ S S
Sbjct: 372 SFH--QELIQIEEDLFTSLGLHFKTLDMATADLGAPAYRKFDIEAWMPGLGRFGEISSAS 431

Query: 421 NCTDYQSRRLEIRY--------GQK--KSNEQTKQYVHLLNSTLTATERTLCCILENYQK 480
           NCTDYQSRRL IRY        G K  K+N    ++VH LN+T  A  R + C+LENYQ+
Sbjct: 432 NCTDYQSRRLGIRYRPSEPPQTGPKKGKANLPATKFVHTLNATACAVPRMMVCLLENYQQ 491

Query: 481 EDG-VEIPEVLQPYMGGKTFLPFKAK 491
           EDG V IPE L+P+MGG   +  K K
Sbjct: 492 EDGSVVIPEPLRPFMGGIELIKPKHK 512

BLAST of HG10007089 vs. TAIR 10
Match: AT1G11870.3 (Seryl-tRNA synthetase )

HSP 1 Score: 137.9 bits (346), Expect = 2.3e-32
Identity = 85/263 (32.32%), Postives = 144/263 (54.75%), Query Frame = 0

Query: 121 EMKKGEDASEKIKDTEENKKLSTEKDVEAKDVLKKLNSKL----EIIGNLVHDSVPISID 180
           +MK   + SE+ +  EE K L  E  V  ++ L KL  +L    + I N+ H  VP+  +
Sbjct: 134 KMKGKLEPSERERLVEEGKNLK-ESLVTLEEDLVKLKDELQHVAQSIPNMTHPDVPVGGE 193

Query: 181 EANNAVIRSWGEKLVKPKLKNHVDLVELLELADTKRGSEIAGGRGYYLIGAGMRLNQALI 240
           +++         +     +K+H+ L + L+L D    +E++G + +YL    + L  AL+
Sbjct: 194 DSSAIRQEVGSPREFSFPIKDHLQLGKDLDLIDFDSAAEVSGSKFFYLKNEAVLLEMALL 253

Query: 241 NYGLDFLEKKGYTPLQTPFFMRKDIMARCAQLAQFDE-ELYKVTGEGDDKYLIATAEQAL 300
           N+ L  + KKGYTPL TP  +R  I+ +C    + D  ++Y +  +G D+ LI TAE  +
Sbjct: 254 NWTLSQVMKKGYTPLTTPEIVRSSIVEKCGFQPRGDNTQVYSI--DGTDQCLIGTAEIPV 313

Query: 301 CAYHLEEWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 360
              H++  +  S LP++Y  +S CFR EAG+ G  T G++RVHQF K E F I  P  ++
Sbjct: 314 GGIHMDSILLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQPEDSE 373

Query: 361 SWDMLEEMIKNSEEFYKTLNIPY 379
           S+   +E+I+  E+ + +L + +
Sbjct: 374 SFH--QELIQIEEDLFTSLGLHF 391

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892840.15.1e-23683.70serine--tRNA ligase isoform X2 [Benincasa hispida] >XP_038892841.1 serine--tRNA ... [more]
XP_022145958.11.9e-23583.70serine--tRNA ligase [Momordica charantia] >XP_022145959.1 serine--tRNA ligase [M... [more]
XP_008464736.19.6e-23583.10PREDICTED: serine--tRNA ligase, cytoplasmic [Cucumis melo] >XP_008464737.1 PREDI... [more]
XP_011654444.11.6e-23483.50serine--tRNA ligase [Cucumis sativus] >XP_031741845.1 serine--tRNA ligase [Cucum... [more]
XP_038892839.11.4e-23382.87serine--tRNA ligase isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q392303.5e-20369.57Serine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g27470 PE... [more]
O819834.5e-19569.59Serine--tRNA ligase OS=Helianthus annuus OX=4232 PE=2 SV=1[more]
O140182.1e-13652.05Serine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q7KWQ29.9e-13450.92Serine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=serS PE... [more]
Q186781.7e-12548.24Probable serine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=s... [more]
Match NameE-valueIdentityDescription
A0A6J1CWS69.4e-23683.70Seryl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015292 PE=4 SV=1[more]
A0A1S3CM544.6e-23583.10Seryl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103502550 PE=4 SV=1[more]
A0A5D3CBX24.6e-23583.10Seryl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold76... [more]
A0A0A0KL587.9e-23583.50Seryl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_5G002590 PE=4 SV=1[more]
A0A6J1EKK73.3e-23382.11Seryl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433427 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G27470.12.5e-20469.57seryl-tRNA synthetase / serine--tRNA ligase [more]
AT1G11870.25.7e-6036.27Seryl-tRNA synthetase [more]
AT1G11870.15.7e-6036.27Seryl-tRNA synthetase [more]
AT1G11870.32.3e-3232.32Seryl-tRNA synthetase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 49..69
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 165..495
e-value: 1.3E-145
score: 486.6
NoneNo IPR availablePANTHERPTHR11778:SF18BNAA09G03770D PROTEINcoord: 124..488
NoneNo IPR availablePANTHERPTHR11778:SF18BNAA09G03770D PROTEINcoord: 1..69
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 177..486
IPR002317Serine-tRNA ligase, type1PRINTSPR00981TRNASYNTHSERcoord: 335..348
score: 65.34
coord: 323..335
score: 66.38
coord: 394..410
score: 61.66
coord: 412..428
score: 67.76
coord: 377..390
score: 45.24
IPR002317Serine-tRNA ligase, type1PIRSFPIRSF001529Ser-tRNA_ligasecoord: 116..488
e-value: 4.0E-161
score: 534.3
coord: 1..79
e-value: 5.7E-16
score: 56.1
IPR002317Serine-tRNA ligase, type1TIGRFAMTIGR00414TIGR00414coord: 123..479
e-value: 1.7E-133
score: 443.7
IPR002317Serine-tRNA ligase, type1PANTHERPTHR11778SERYL-TRNA SYNTHETASEcoord: 1..69
coord: 124..488
IPR042103Serine-tRNA synthetase, type1, N-terminal domain superfamilyGENE3D1.10.287.40coord: 1..71
e-value: 2.4E-30
score: 107.4
IPR015866Serine-tRNA synthetase, type1, N-terminalPFAMPF02403Seryl_tRNA_Ncoord: 1..66
e-value: 1.6E-18
score: 66.9
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 276..463
e-value: 1.3E-37
score: 129.5
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 197..472
score: 22.794161
IPR033729Serine-tRNA ligase catalytic core domainCDDcd00770SerRS_corecoord: 179..479
e-value: 0.0
score: 519.039
IPR010978Class I and II aminoacyl-tRNA synthetase, tRNA-binding armSUPERFAMILY46589tRNA-binding armcoord: 1..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10007089.1HG10007089.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006434 seryl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005829 cytosol
molecular_function GO:0005524 ATP binding
molecular_function GO:0004828 serine-tRNA ligase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding