HG10007019 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10007019
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	nicotianamine synthase-like
Location	Chr10: 406865 .. 408549 (-)
RNA-Seq Expression	HG10007019
Synteny	HG10007019

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTTGTGAAGAAGAAGCTCTAATTCAAAAAGTTAGTGAATTATATGATGAAATCTCAAGCCTTGAAAGCCTCAAACCTTGTAACCATGTCGACACTCTCTTCACTAAGTTGGTGGTCACGTGCATGTCACCCACCTCACCTCATTTCCATATCAATTCACTTTCGAAACCACTTCAACAAATGAGATCAAATCTCATTAGCCTTTGTGGTCAAGCTGAGTCTCTTTTAGAGTCTCATTTTTCTGATCTCTTAGCCAAATTCGATTCCCCTATTCACCATCTTCATGTCTTCCCTTACTTCTCTAATTATCTTAAACTTAGTCTCCTTGAATTCTCCATCCTTTCCCGCCACTGCCCCGTCGTTCCCTCCTCCGTCGCCTTCGTTGGCTCTGGTCCTCTTCCTCTTACCTCCATCGTCCTCGCCACCCGCCACCTCACCTCCACTATCTTCCACAACTACGATCTTGACCCGCTTGCTAATTCCAAGGTTATATATGTTGGTTCATTTAATTTAGTGTAATGAACTTTTGAATTTAGCACCATGTTTTTAACTCTCTGATTAATTTTGATAACTACGTGTTTGGTATAGCTGTCTGTTTTAGAAATTTATATGTTTGTTTTCTTCCTCTTTTTCGGTTATGGTTTTCACCTTTCTTAAAGAAACCCTTAAAATTTTAGCTAAATTCTAAGAACAAAAACGAAACTGTTTTTTAAAACTTGTTTGGCAACTCTTTAACCTTTGGTTTTTAGTTTTTTAAAATTAAGCTTATAAAAACCATTTTGATCACCTTTAAATTTCTTGCTTTGTTTTCTATTTTTTTACCGATATTTTCAAAAACTGAAACAAGTTCTGAAATTCAAAAAACAGTTTTTGAAAATTTATTTTTGTTTTTGGAATTAAGCTAAGAAATCAACTCTTATACTTATGAAAGATGGAAAACATCACAAGAAATTTAATTTTCAAAACTTAACTTGATTTTTAAAAATAAACCTAGGACGAACTTATGATTAGAACAAAGAAATAATGTTTATAAGCTTAATTTTTCCCAAAAACCAGATGTTTATCAAACAACACCGGAAGTAAACTGAACTTGTATTTGCTTCCCACAGGCGTCGAATTTGGTGTCAGTCGACTCGGACCTGAAGACGAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACAGAGGAGCTGAAACAGTACGAGGTTGTGTTTTTGGCAGCTCTTGTAGGAATGGAGAAAGAGGAGAAATTGAAAGTGATTAAGCATTTGTCAGAGTTCATGTCAGAAGGGGCTTATTTGATGGTAAGAAGTGCACATGGAGGAAGAGCTTTTCTATATCCTGTTGTTGAAGATTGTGATTTGGTAGGGTTTGAGGTTTTATCTGTGTTTCATCCCACTGATGAAGTTATAAACTCAGTAATAATTGCACGAAAAAAGACGACTTTCGATAACGATAACATTAACGACAACGACAACGATAACAACGATGACGAAGAAGGCTGTTTGTCTGTTAATTTAGACAAACAAGAGGAAGATGAAAATAAGAAGGTGAAGATTAGCAGTGGAATTGATCATAAGAATAATAATGGAGTTGTGCATAATAACAAATGTTCTGAGATTCAAAGCTTCAATAACCATGGAGGTAAGATTGAGGAATTTGCCATGGAAGCTGAGTAG

mRNA sequence

ATGGTTTGTGAAGAAGAAGCTCTAATTCAAAAAGTTAGTGAATTATATGATGAAATCTCAAGCCTTGAAAGCCTCAAACCTTGTAACCATGTCGACACTCTCTTCACTAAGTTGGTGGTCACGTGCATGTCACCCACCTCACCTCATTTCCATATCAATTCACTTTCGAAACCACTTCAACAAATGAGATCAAATCTCATTAGCCTTTGTGGTCAAGCTGAGTCTCTTTTAGAGTCTCATTTTTCTGATCTCTTAGCCAAATTCGATTCCCCTATTCACCATCTTCATGTCTTCCCTTACTTCTCTAATTATCTTAAACTTAGTCTCCTTGAATTCTCCATCCTTTCCCGCCACTGCCCCGTCGTTCCCTCCTCCGTCGCCTTCGTTGGCTCTGGTCCTCTTCCTCTTACCTCCATCGTCCTCGCCACCCGCCACCTCACCTCCACTATCTTCCACAACTACGATCTTGACCCGCTTGCTAATTCCAAGGCGTCGAATTTGGTGTCAGTCGACTCGGACCTGAAGACGAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACAGAGGAGCTGAAACAGTACGAGGTTGTGTTTTTGGCAGCTCTTGTAGGAATGGAGAAAGAGGAGAAATTGAAAGTGATTAAGCATTTGTCAGAGTTCATGTCAGAAGGGGCTTATTTGATGGTAAGAAGTGCACATGGAGGAAGAGCTTTTCTATATCCTGTTGTTGAAGATTGTGATTTGGTAGGGTTTGAGGTTTTATCTGTGTTTCATCCCACTGATGAAGTTATAAACTCAGTAATAATTGCACGAAAAAAGACGACTTTCGATAACGATAACATTAACGACAACGACAACGATAACAACGATGACGAAGAAGGCTGTTTGTCTGTTAATTTAGACAAACAAGAGGAAGATGAAAATAAGAAGGTGAAGATTAGCAGTGGAATTGATCATAAGAATAATAATGGAGTTGTGCATAATAACAAATGTTCTGAGATTCAAAGCTTCAATAACCATGGAGGTAAGATTGAGGAATTTGCCATGGAAGCTGAGTAG

Coding sequence (CDS)

ATGGTTTGTGAAGAAGAAGCTCTAATTCAAAAAGTTAGTGAATTATATGATGAAATCTCAAGCCTTGAAAGCCTCAAACCTTGTAACCATGTCGACACTCTCTTCACTAAGTTGGTGGTCACGTGCATGTCACCCACCTCACCTCATTTCCATATCAATTCACTTTCGAAACCACTTCAACAAATGAGATCAAATCTCATTAGCCTTTGTGGTCAAGCTGAGTCTCTTTTAGAGTCTCATTTTTCTGATCTCTTAGCCAAATTCGATTCCCCTATTCACCATCTTCATGTCTTCCCTTACTTCTCTAATTATCTTAAACTTAGTCTCCTTGAATTCTCCATCCTTTCCCGCCACTGCCCCGTCGTTCCCTCCTCCGTCGCCTTCGTTGGCTCTGGTCCTCTTCCTCTTACCTCCATCGTCCTCGCCACCCGCCACCTCACCTCCACTATCTTCCACAACTACGATCTTGACCCGCTTGCTAATTCCAAGGCGTCGAATTTGGTGTCAGTCGACTCGGACCTGAAGACGAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACAGAGGAGCTGAAACAGTACGAGGTTGTGTTTTTGGCAGCTCTTGTAGGAATGGAGAAAGAGGAGAAATTGAAAGTGATTAAGCATTTGTCAGAGTTCATGTCAGAAGGGGCTTATTTGATGGTAAGAAGTGCACATGGAGGAAGAGCTTTTCTATATCCTGTTGTTGAAGATTGTGATTTGGTAGGGTTTGAGGTTTTATCTGTGTTTCATCCCACTGATGAAGTTATAAACTCAGTAATAATTGCACGAAAAAAGACGACTTTCGATAACGATAACATTAACGACAACGACAACGATAACAACGATGACGAAGAAGGCTGTTTGTCTGTTAATTTAGACAAACAAGAGGAAGATGAAAATAAGAAGGTGAAGATTAGCAGTGGAATTGATCATAAGAATAATAATGGAGTTGTGCATAATAACAAATGTTCTGAGATTCAAAGCTTCAATAACCATGGAGGTAAGATTGAGGAATTTGCCATGGAAGCTGAGTAG

Protein sequence

MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQQMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCPVVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAFLYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSVNLDKQEEDENKKVKISSGIDHKNNNGVVHNNKCSEIQSFNNHGGKIEEFAMEAE

Homology

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BLAST of HG10007019 vs. NCBI nr
Match: XP_038878580.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 575.5 bits (1482), Expect = 3.2e-160
Identity = 304/369 (82.38%), Postives = 321/369 (86.99%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSK LQ
Sbjct: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKSLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP 120
           QMRSNLI LCGQAESLLE HFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSIL RHCP
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMVF 180
           V+PS+VAFVGSGPLPLTSIVLATRHLTST+FHNYDLD LANSKASNLVS+D DLKTRMVF
Sbjct: 121 VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDLLANSKASNLVSLDPDLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELKQYEVVFLAALVGMEKEEKL+VIKHLSEFMSEGAYLMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIKHLSEFMSEGAYLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDND------------ 300
           YPVVEDCDLVGFEVLSVFHPTDEVINSVIIA+KK  FD++N ND++ D            
Sbjct: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIAQKKMIFDDNNDNDDNRDYLSVNSDKLEED 300

Query: 301 ----------------------NNDDEEGCLSVNLDKQEEDENKKVKISSGIDHKNNNGV 336
                                 ++DD++GCLSVN  KQE+DEN+KV +S GIDHKNNNGV
Sbjct: 301 ENEKVMISGFDRKNNGVMHNDKDSDDDDGCLSVNSSKQEKDENEKVMLSGGIDHKNNNGV 360

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BLAST of HG10007019 vs. NCBI nr
Match: XP_008450545.1 (PREDICTED: nicotianamine synthase-like [Cucumis melo])

HSP 1 Score: 573.9 bits (1478), Expect = 9.3e-160
Identity = 310/356 (87.08%), Postives = 328/356 (92.13%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEE LIQ+V ELYDEISSLESLKPCN+VDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQRVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP 120
           QMRS+LISLCGQAESLLESHFSDLLAKFDSPI HLHVFPYFSNYLKLSLLEFSIL RH P
Sbjct: 61  QMRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120

Query: 121 -VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMV 180
            V+PS+VAFVGSGPLPLTSIVLATRHLTST+FHNYDLDP+ANSKASNLVS+D DLKTRMV
Sbjct: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEK+EKLKVIKHLS++MSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSV 300
           LYPVVED DL+GFEVLSVFHPTDEVINSVIIARK T +DN+N   + NDNNDD   CL V
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNEN---DSNDNNDD-NCCLFV 300

Query: 301 NLDKQEEDENKKVKISSGIDH-KNNNGVVHNNKCSEIQS--FNNHGGKIEEFAMEA 353
           N      DEN+KVKISSGIDH K+NNGVVHNNKCSEIQ+   NNHGGKIEEFAMEA
Sbjct: 301 N-----SDENEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEA 347

[Close]

BLAST of HG10007019 vs. NCBI nr
Match: XP_004135580.1 (nicotianamine synthase [Cucumis sativus] >KGN66010.1 hypothetical protein Csa_007577 [Cucumis sativus])

HSP 1 Score: 558.5 bits (1438), Expect = 4.0e-155
Identity = 302/354 (85.31%), Postives = 317/354 (89.55%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEE LIQKV ELYDEISSLESLKPCN+VDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP 120
           QMRSNLI LCGQAESLLE HFSDLLAKFDSPI HLHVFPYFSNY+KLSLLEFSIL RH P
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 -VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMV 180
             +PS+VAFVGSGPLPLTS+VLATRHLTST+FHNYDLDP+ANSKASNLVS D DLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSE+MSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSV 300
           LYPVVED DL+GFEVLSVFHPTDEVINSVIIARK   FDND  ND+D++NN+D   CL V
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDND--NDSDDNNNNDNGCCLFV 300

Query: 301 NLDKQEEDENKKVKISSGIDHKNNNGVVHNNKCSEIQS-FNNHGGKIEEFAMEA 353
           N      DEN+ VKISS        GVVHNNKCSEIQ+ FNNHGGKIEEFAMEA
Sbjct: 301 N-----SDENENVKISS--------GVVHNNKCSEIQNGFNNHGGKIEEFAMEA 339

[Close]

BLAST of HG10007019 vs. NCBI nr
Match: XP_023529837.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 499.2 bits (1284), Expect = 2.9e-137
Identity = 268/351 (76.35%), Postives = 293/351 (83.48%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEEALIQKV +LY++IS LESLKPC HVDTLFT+LVVTCM PTSP+FHINSLS P+Q
Sbjct: 1   MVTEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPVQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP 120
           QMRSNLI LCGQAESLLESHFSDLLA FD P+HHLH+FPYFSNYLKLSLLEFSIL RHCP
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMVF 180
           V+PSSVAFVGSGPLPLTSIVLATRHL ST FHNYDLDPLANS+AS LVSVD+DLKTRMVF
Sbjct: 121 VLPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDADLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL EFM+EGA LMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSVN 300
           YPVVE+CDLVGFEVLSVFHP+DEVINSVIIARKK              ++DD++ CL + 
Sbjct: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKK------------KKDHDDDDDCLKIV 300

Query: 301 LDKQEEDENKKVKISSGIDHKNNNGVVHNNK--CSEIQSFNNHGGKIEEFA 350
            D   E         +GI+    NGVV +NK  C  I  FNNHGG IEE A
Sbjct: 301 GDDNHE---------NGIE----NGVVVHNKQCCDHIHGFNNHGGMIEELA 326

[Close]

BLAST of HG10007019 vs. NCBI nr
Match: KAG6588596.1 (putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 497.3 bits (1279), Expect = 1.1e-136
Identity = 269/351 (76.64%), Postives = 294/351 (83.76%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEEALIQKV +LY++IS LESLKPC HVDTLFT+LVVTCM+PTSP+FHINSLS PLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMAPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP 120
           QMRSNLI LCGQAESLLESHFSDLLA FD P+HHLH+FPYFSNYLKLSLLEFSIL RHCP
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMVF 180
           V+PSSVAFVGSGPLPLTSIVLATRHL ST FHNYD+DPLAN++AS LVSVDSDLKTRMVF
Sbjct: 121 VLPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDIDPLANTQASKLVSVDSDLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL EFM+EGA LMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSVN 300
           YPVVE+CDLVGFEVLSVFHP+DEVINSVIIARKK             D++DD+  CL + 
Sbjct: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKK-----------KKDHDDDD--CLKIV 300

Query: 301 LDKQEEDENKKVKISSGIDHKNNNGVVHNNK--CSEIQSFNNHGGKIEEFA 350
            D   E         +GI+    NGVV +NK  C  I  FNNHGG IEE A
Sbjct: 301 GDDNHE---------NGIE----NGVVVHNKQCCDHIHGFNNHGGMIEELA 325

[Close]

BLAST of HG10007019 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.8e-90
Identity = 177/314 (56.37%), Postives = 235/314 (74.84%), Query Frame = 0


Query: 3   CEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQQM 62
           C+++ L+ K+ +LY++IS LE+LKPC  VDTLF +LV TC+ P +P+  +  +S+ +Q+M
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI-PPNPNIDVTKMSENIQEM 63

Query: 63  RSNLISLCGQAESLLESHFSDLLAKF-DSPIHHLHVFPYFSNYLKLSLLEFSILSRHC-P 122
           RSNLI +CG+AE  LE HFS +L  F D+P+HHL++FPY++NYLKLS LEF +L ++   
Sbjct: 64  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 123

Query: 123 VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMVF 182
            VP +VAF+GSGPLPLTS+VLA+ HL  +IFHN+D+DP AN  A+ LVS D DL  RM F
Sbjct: 124 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 183

Query: 183 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAFL 242
           HT DIM VTE LK ++VVFLAALVGM+K+EK+KV++HL + MS GA LM+RSAHG RAFL
Sbjct: 184 HTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFL 243

Query: 243 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSVN 302
           YP+VE CDL GFEVLSV+HPTDEVINS++I+RK     N  ++D+ +  +D     L+ N
Sbjct: 244 YPIVEPCDLEGFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASD-----LACN 303

Query: 303 LDKQEEDENKKVKI 315
             K     NKK  I
Sbjct: 304 CSKIHVIMNKKKSI 311

[Close]

BLAST of HG10007019 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.1e-89
Identity = 166/274 (60.58%), Postives = 216/274 (78.83%), Query Frame = 0


Query: 1   MVC-EEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPL 60
           MVC     +++KV ELY++IS LE+L P   V+ LFT LV TCM P      ++ L + +
Sbjct: 1   MVCPNSNPVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP--IDVSKLCQKI 60

Query: 61  QQMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHC 120
           Q++RS+LI LCGQAE LLESHFS +L+ +++P+ HLH+FPYF NY+KLSLLE++IL+++ 
Sbjct: 61  QEIRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNT 120

Query: 121 PVVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMV 180
             +P  +AF+GSGPLPLTS+VLAT+HL +T FHNYD+D  AN  AS LV+ D D+ +RM 
Sbjct: 121 TNIPKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMT 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAF 240
           FHT D+M VT  LK Y+VVFLAALVGM+KE+K+KV+ HL+++MS GA LM+RSAHG RAF
Sbjct: 181 FHTADVMDVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARK 274
           LYPV++  DL GFEVLSV+HPTDEVINSVIIARK
Sbjct: 241 LYPVLDPRDLRGFEVLSVYHPTDEVINSVIIARK 272

[Close]

BLAST of HG10007019 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.3e-87
Identity = 166/284 (58.45%), Postives = 216/284 (76.06%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C++E L+Q + +LY++IS LESLKP   V+ LF +LV TC+ P +P+  +  +   +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI-PPNPNIDVTKMCDRVQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKF-DSPIHHLHVFPYFSNYLKLSLLEFSILSRHC 120
           ++R NLI +CG AE  LE+HFS +L  + D+P+HHL++FPY++NYLKL  LEF +L ++ 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 -PVVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRM 180
              VP SVAF+GSGPLPLTSIVLA+ HL  TIFHN+D+DP ANS AS LVS D D+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRA 240
            FHT DIM VTE LK ++VVFLAALVGM KEEK+KVI+HL + M+ GA LM+RSAHG RA
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 240

Query: 241 FLYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNI 283
           FLYP+VE CDL GFEVLS++HPTD+VINSV+I++K       N+
Sbjct: 241 FLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKKHPVVSIGNV 283

[Close]

BLAST of HG10007019 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.9e-87
Identity = 159/275 (57.82%), Postives = 215/275 (78.18%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSL-SKPL 60
           M CE   +++++ +LY++IS+LESLKP  +VDTLF +LV TC+ PT  +  +  +  + +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLISLCGQAESLLESHFSDLLAKF-DSPIHHLHVFPYFSNYLKLSLLEFSILSRH 120
           + MRS+LI LCG+AE  LE HFS +L  F D+P++HLH+FPY++NYLKL  LEF +LS+H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 CPVVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRM 180
              VP+ VAF+GSGP+PLTSIVLA  HL +T FHN+D+D  AN+ ASNLVS DSDL  RM
Sbjct: 121 TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRA 240
           +FHT D++   E L QY+VVFLAALVGM+KE K+K I+HL + M+ GA +M+RSAHG RA
Sbjct: 181 IFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRA 240

Query: 241 FLYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARK 274
           FLYP+V+ CDL GFEVL+++HP+D+V+NSV+IARK
Sbjct: 241 FLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of HG10007019 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.7e-85
Identity = 155/276 (56.16%), Postives = 210/276 (76.09%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C+   +++++ +LYD+IS L+SLKP  +VDTLF +LV TC+ PT  +  + ++ + ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVK 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKF---DSPIHHLHVFPYFSNYLKLSLLEFSILSR 120
            MR+NLI LCG+AE  LE HFS +L       +P+ HLH+FPY+SNYLKL  LEF +LS+
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HCPVVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTR 180
           H   VP+ +AFVGSGP+PLTSIVLA  HL +T FHN+D+D  AN+ ASNLVS D DL  R
Sbjct: 121 HSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKR 180

Query: 181 MVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGR 240
           M+FHT D++  TE L QY+VVFLAALVGM+KE K+K I+HL + M+ GA LM+RSAH  R
Sbjct: 181 MIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALR 240

Query: 241 AFLYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARK 274
           AFLYP+V+  DL GF++L+++HPTD+V+NSV+IARK
Sbjct: 241 AFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of HG10007019 vs. ExPASy TrEMBL
Match: A0A1S3BPH1 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103492113 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.5e-160
Identity = 310/356 (87.08%), Postives = 328/356 (92.13%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEE LIQ+V ELYDEISSLESLKPCN+VDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQRVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP 120
           QMRS+LISLCGQAESLLESHFSDLLAKFDSPI HLHVFPYFSNYLKLSLLEFSIL RH P
Sbjct: 61  QMRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120

Query: 121 -VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMV 180
            V+PS+VAFVGSGPLPLTSIVLATRHLTST+FHNYDLDP+ANSKASNLVS+D DLKTRMV
Sbjct: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEK+EKLKVIKHLS++MSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSV 300
           LYPVVED DL+GFEVLSVFHPTDEVINSVIIARK T +DN+N   + NDNNDD   CL V
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNEN---DSNDNNDD-NCCLFV 300

Query: 301 NLDKQEEDENKKVKISSGIDH-KNNNGVVHNNKCSEIQS--FNNHGGKIEEFAMEA 353
           N      DEN+KVKISSGIDH K+NNGVVHNNKCSEIQ+   NNHGGKIEEFAMEA
Sbjct: 301 N-----SDENEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEA 347

[Close]

BLAST of HG10007019 vs. ExPASy TrEMBL
Match: A0A0A0LVX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G561410 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 2.0e-155
Identity = 302/354 (85.31%), Postives = 317/354 (89.55%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEE LIQKV ELYDEISSLESLKPCN+VDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP 120
           QMRSNLI LCGQAESLLE HFSDLLAKFDSPI HLHVFPYFSNY+KLSLLEFSIL RH P
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 -VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMV 180
             +PS+VAFVGSGPLPLTS+VLATRHLTST+FHNYDLDP+ANSKASNLVS D DLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSE+MSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSV 300
           LYPVVED DL+GFEVLSVFHPTDEVINSVIIARK   FDND  ND+D++NN+D   CL V
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDND--NDSDDNNNNDNGCCLFV 300

Query: 301 NLDKQEEDENKKVKISSGIDHKNNNGVVHNNKCSEIQS-FNNHGGKIEEFAMEA 353
           N      DEN+ VKISS        GVVHNNKCSEIQ+ FNNHGGKIEEFAMEA
Sbjct: 301 N-----SDENENVKISS--------GVVHNNKCSEIQNGFNNHGGKIEEFAMEA 339

[Close]

BLAST of HG10007019 vs. ExPASy TrEMBL
Match: A0A6J1EU11 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111437742 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 7.0e-137
Identity = 267/350 (76.29%), Postives = 290/350 (82.86%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEEALIQKV +LY++IS LESLKPC HVDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP 120
           QMRSNLI LCGQAESLLESHFSDLLA FD P+HHLH+FPYFSNYLKLSLLEFSIL RHCP
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMVF 180
           V+PSSVAFVGSGPLPLTSIVLATRHL ST FHNYDLDPLAN++AS LVSVDSDLKTRMVF
Sbjct: 121 VLPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANTQASKLVSVDSDLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL EFM+EGA LMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSVN 300
           YPVVE+CDLVGFEVLSVFHP+DEVINSVIIARKK             D++DD+  CL + 
Sbjct: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKK-----------KKDHDDDD--CLKI- 300

Query: 301 LDKQEEDENKKVKISSGIDHKNNNGVVHNNKCSE-IQSFNNHGGKIEEFA 350
                           G D+  N  VVHN +C + I  FNNHGG IEE A
Sbjct: 301 ---------------VGDDNHENGVVVHNKQCCDHIHGFNNHGGMIEELA 321

[Close]

BLAST of HG10007019 vs. ExPASy TrEMBL
Match: A0A6J1JPC1 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111486493 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.2e-136
Identity = 269/351 (76.64%), Postives = 290/351 (82.62%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEEALIQKV +LY++IS LESLKPC HVDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP 120
           QMRSNLI LCGQAESLLESHFSDLLA FD P+HHLH+FPYFSNYLKLSLLEFSIL RHCP
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMVF 180
           ++PSSVAFVGSGPLPLTSIVLATRHL ST FHNYDLDPLANS+AS LVSVDSDLKTRMVF
Sbjct: 121 ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVF 180

Query: 181 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAFL 240
           HTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL EFM+EGA LMVRSAHGGRAFL
Sbjct: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240

Query: 241 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSVN 300
           YPVVE+CDLVGFEVLSVFHP+DEVINSVIIARKK                DD+  CL + 
Sbjct: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKK--------------KKDDD--CLKIV 300

Query: 301 LDKQEEDENKKVKISSGIDHKNNNGVVHNNK--CSEIQSFNNHGGKIEEFA 350
            D   E         +GI+    NGVV +NK  C  I  FNNHGG IEE A
Sbjct: 301 GDDNHE---------NGIE----NGVVVHNKQCCDHIHGFNNHGGMIEELA 322

[Close]

BLAST of HG10007019 vs. ExPASy TrEMBL
Match: A0A5A7U6Y1 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004490 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 3.3e-126
Identity = 253/295 (85.76%), Postives = 269/295 (91.19%), Query Frame = 0


Query: 62  MRSNLISLCGQAESLLESHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILSRHCP- 121
           MRS+LISLCGQAESLLESHFSDLLAKFDSPI HLHVFPYFSNYLKLSLLEFSIL RH P 
Sbjct: 1   MRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR 60

Query: 122 VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMVF 181
           V+PS+VAFVGSGPLPLTSIVLATRHLTST+FHNYDLDP+ANSKASNLVS+D DLKTRMVF
Sbjct: 61  VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF 120

Query: 182 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAFL 241
           HTCDIMKVTEELKQYEVVFLAALVGMEK+EKLKVIKHLS++MSEGAYLMVRSAHGGRAFL
Sbjct: 121 HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL 180

Query: 242 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSVN 301
           YPVVED DL+GFEVLSVFHPTDEVINSVIIARK T +DN+N   + NDNNDD   CL VN
Sbjct: 181 YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNEN---DSNDNNDD-NCCLFVN 240

Query: 302 LDKQEEDENKKVKISSGIDH-KNNNGVVHNNKCSEIQS--FNNHGGKIEEFAMEA 353
                 DEN+KVKISSGIDH K+NNGVVHNNKCSEIQ+   NNHGGKIEEFAMEA
Sbjct: 241 -----SDENEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEA 286

[Close]

BLAST of HG10007019 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 333.6 bits (854), Expect = 2.0e-91
Identity = 177/314 (56.37%), Postives = 235/314 (74.84%), Query Frame = 0


Query: 3   CEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQQM 62
           C+++ L+ K+ +LY++IS LE+LKPC  VDTLF +LV TC+ P +P+  +  +S+ +Q+M
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI-PPNPNIDVTKMSENIQEM 63

Query: 63  RSNLISLCGQAESLLESHFSDLLAKF-DSPIHHLHVFPYFSNYLKLSLLEFSILSRHC-P 122
           RSNLI +CG+AE  LE HFS +L  F D+P+HHL++FPY++NYLKLS LEF +L ++   
Sbjct: 64  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 123

Query: 123 VVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRMVF 182
            VP +VAF+GSGPLPLTS+VLA+ HL  +IFHN+D+DP AN  A+ LVS D DL  RM F
Sbjct: 124 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 183

Query: 183 HTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRAFL 242
           HT DIM VTE LK ++VVFLAALVGM+K+EK+KV++HL + MS GA LM+RSAHG RAFL
Sbjct: 184 HTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFL 243

Query: 243 YPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNINDNDNDNNDDEEGCLSVN 302
           YP+VE CDL GFEVLSV+HPTDEVINS++I+RK     N  ++D+ +  +D     L+ N
Sbjct: 244 YPIVEPCDLEGFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASD-----LACN 303

Query: 303 LDKQEEDENKKVKI 315
             K     NKK  I
Sbjct: 304 CSKIHVIMNKKKSI 311

[Close]

BLAST of HG10007019 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 324.7 bits (831), Expect = 9.2e-89
Identity = 166/284 (58.45%), Postives = 216/284 (76.06%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C++E L+Q + +LY++IS LESLKP   V+ LF +LV TC+ P +P+  +  +   +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI-PPNPNIDVTKMCDRVQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKF-DSPIHHLHVFPYFSNYLKLSLLEFSILSRHC 120
           ++R NLI +CG AE  LE+HFS +L  + D+P+HHL++FPY++NYLKL  LEF +L ++ 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 -PVVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRM 180
              VP SVAF+GSGPLPLTSIVLA+ HL  TIFHN+D+DP ANS AS LVS D D+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRA 240
            FHT DIM VTE LK ++VVFLAALVGM KEEK+KVI+HL + M+ GA LM+RSAHG RA
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 240

Query: 241 FLYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARKKTTFDNDNI 283
           FLYP+VE CDL GFEVLS++HPTD+VINSV+I++K       N+
Sbjct: 241 FLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKKHPVVSIGNV 283

[Close]

BLAST of HG10007019 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 323.6 bits (828), Expect = 2.0e-88
Identity = 159/275 (57.82%), Postives = 215/275 (78.18%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSL-SKPL 60
           M CE   +++++ +LY++IS+LESLKP  +VDTLF +LV TC+ PT  +  +  +  + +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLISLCGQAESLLESHFSDLLAKF-DSPIHHLHVFPYFSNYLKLSLLEFSILSRH 120
           + MRS+LI LCG+AE  LE HFS +L  F D+P++HLH+FPY++NYLKL  LEF +LS+H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 CPVVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTRM 180
              VP+ VAF+GSGP+PLTSIVLA  HL +T FHN+D+D  AN+ ASNLVS DSDL  RM
Sbjct: 121 TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGRA 240
           +FHT D++   E L QY+VVFLAALVGM+KE K+K I+HL + M+ GA +M+RSAHG RA
Sbjct: 181 IFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRA 240

Query: 241 FLYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARK 274
           FLYP+V+ CDL GFEVL+++HP+D+V+NSV+IARK
Sbjct: 241 FLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of HG10007019 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 317.0 bits (811), Expect = 1.9e-86
Identity = 155/276 (56.16%), Postives = 210/276 (76.09%), Query Frame = 0


Query: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C+   +++++ +LYD+IS L+SLKP  +VDTLF +LV TC+ PT  +  + ++ + ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVK 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKF---DSPIHHLHVFPYFSNYLKLSLLEFSILSR 120
            MR+NLI LCG+AE  LE HFS +L       +P+ HLH+FPY+SNYLKL  LEF +LS+
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HCPVVPSSVAFVGSGPLPLTSIVLATRHLTSTIFHNYDLDPLANSKASNLVSVDSDLKTR 180
           H   VP+ +AFVGSGP+PLTSIVLA  HL +T FHN+D+D  AN+ ASNLVS D DL  R
Sbjct: 121 HSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKR 180

Query: 181 MVFHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEFMSEGAYLMVRSAHGGR 240
           M+FHT D++  TE L QY+VVFLAALVGM+KE K+K I+HL + M+ GA LM+RSAH  R
Sbjct: 181 MIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALR 240

Query: 241 AFLYPVVEDCDLVGFEVLSVFHPTDEVINSVIIARK 274
           AFLYP+V+  DL GF++L+++HPTD+V+NSV+IARK
Sbjct: 241 AFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIARK 275

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BLAST of HG10007019 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-10
Identity = 32/56 (57.14%), Postives = 41/56 (73.21%), Query Frame = 0


Query: 222 MSEGAYLMVRSAHGGRAFLYPVVEDCDLVGFEVLSVFHP--TDEVINSVIIARKKT 276
           M  GA LM+RSA G R+FLY  V+ CDL GFEVL ++HP  +D  +NSV++ARK T
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARKLT 56

The following BLAST results are available for this feature:

BLAST of HG10007019 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878580.1	3.2e-160	82.38	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
XP_008450545.1	9.3e-160	87.08	PREDICTED: nicotianamine synthase-like [Cucumis melo]	[more]
XP_004135580.1	4.0e-155	85.31	nicotianamine synthase [Cucumis sativus] >KGN66010.1 hypothetical protein Csa_00...	[more]
XP_023529837.1	2.9e-137	76.35	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6588596.1	1.1e-136	76.64	putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of HG10007019 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	2.8e-90	56.37	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9XGI7	1.1e-89	60.58	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
O80483	1.3e-87	58.45	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FKT9	2.9e-87	57.82	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]
Q9FF79	2.7e-85	56.16	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]

BLAST of HG10007019 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BPH1	4.5e-160	87.08	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103492113 PE=3 SV=1	[more]
A0A0A0LVX1	2.0e-155	85.31	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G561410 PE=3 SV=1	[more]
A0A6J1EU11	7.0e-137	76.29	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111437742 PE=3 S...	[more]
A0A6J1JPC1	1.2e-136	76.64	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111486493 PE=3 SV=...	[more]
A0A5A7U6Y1	3.3e-126	85.76	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]

BLAST of HG10007019 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	2.0e-91	56.37	nicotianamine synthase 4	[more]
AT1G09240.1	9.2e-89	58.45	nicotianamine synthase 3	[more]
AT5G56080.1	2.0e-88	57.82	nicotianamine synthase 2	[more]
AT5G04950.1	1.9e-86	56.16	nicotianamine synthase 1	[more]
AT4G26483.1	2.2e-10	57.14	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 5..275 e-value: 2.3E-117 score: 391.3
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..275
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..278 score: 54.666695
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 6..275 e-value: 2.5E-59 score: 202.6
None	No IPR available	PANTHER	PTHR32266:SF17	SYNTHASE, PUTATIVE-RELATED	coord: 1..275

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10007019.1	HG10007019.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity