HG10006806 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10006806
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionthermospermine synthase ACAULIS5-like
LocationChr07: 22176029 .. 22178396 (-)
RNA-Seq ExpressionHG10006806
SyntenyHG10006806
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTCTTCCTTCTGCTCTGCCCTTTCATTTCTCTTTATCCCCAACTCCACTTCTCTCCCTCTCGATCATATCCACCAAGTATCTGAATGGTTCGAGGAACAACTCGAGGATGATCTCAAATGGTCCTTTGCCGTCAACAGGTCCAATTTTTCTCTCTTTTTCTCTTGCTCATAAGGTGTTTGTTAAAATGTTTTCCTCTTTTATTGCTCGTGGATTCATTTTTGGATGATATAATATTAAGGGTGTGTTTGGAATAAAATGTTTTATTTAAAAAGTAAGTCATTTTGAAAAAAATTGGAGTGATTGTGTACCACTCAAAATATCTTTTAAAGTGTATTTTAAAGTGTATTTTAAACTCTTAAAAAGAGTTTAAATAAAAATGAGTTTGTTTGAAAAACACTTTTTTCTCTATTCAATCCAAAACGATCTTAAATTTAATTTCACTCATTAACTTAAGCTCTTGGATTCAATGCGGTCTAAAGAGATACTACGTTCAAATTCCTACCATGCCTAATTCCTACCATGCCTACATGTGGGGGAGTGTTAAATTTGCTTTCATCCCTGATTTAAGATTCATCTTTTGTAGAGTGGATGGAAAATTTGAGCGTGTTCTTTAAGTTAAAACTGATCCTAACATTATCACGTCCTTTAGGATACTTCATGCCGCAACTAGCGAGTTCCAAGATATAGTTCTGTTGGATACCAAGCGATTTGGGAAGGTTTGCATTATCCCATATCTGTTTCAGTTTCCTTTCCAATAACCCTTGATTAATTTAATACAAATTAGCTGAAAACGAGTTGACATTGATTTCAGGCTCTATTGCTTGATGGGAAGCTACAAAGTGCTGAAAAAGATGAATTCATTTATCATGAGTCCTTGGTTCATCCTGCTCTGTTACTGCATCATAAGTATATGCTGTGTTCATATTTGGTTTAGCTCTTTTAACTGAGCTGAGATGATTTTTGTTGTTTTTATCTTTCCTTTTGATTTTTTTTTTTTTTTTTTTTTGGGAATGATTTTCAGCCCCAAAACAGTGTTTATAATGGGAGGTGGAGAAGGGTCTACTGCTAGAGAGACACTAAAGCATAAAAATCTTGAGGAAGTTGTCATGTGCGATATTGATAGGGTATGGTTCTCAAAATCCTAAGATTCACTCGTTTGCTAATTAATGTGGCAAAAGGATCAATGAAGTTTAGTTCTATGTCTTGAAAAATTATGTAGTAGAGACATGTTGATGGAAGGAAAAGAGAATAACAATGAGAAATGCTAGTTTTTGCATGAAGTCTTTAATTTACATTGTAGGATGTTGTCAATTTCTGCCGCACACATCTAAAAGAAAACCAAGATGCATTTCAGGACGAGAAGCTTCATATTATTTTTGACGATGCAAAGTGAGTAGCTTTTGCCCTTTGGACTGAATCTACCATTGATGTTAAGCTTGTTAAAACTATATAATATCTCAACTTTTGCTTGTATCAATGAACATTTCAAAAGGATTCAAGTTTTGTAAGTTTAGATTTGAAAGAACTAACCCGAAAATGAAAAAGGGCTGGATTGGAGGGACGACCAGAGAAGTTCGATGTAATCATTGGCGATTTATCAGATCCACATGAAGGAGGACCATGCAATCATCTGTACACTAAATCCTTCTATGAGGAAATGATTAAGCCCAAGCTCAATGACAATGGTATTTTTGTTACTCAGGTACATTATATAAGATACTCAAAGCTCTCTGCTATTATTCCTGCTAATTACTACTTTTACTAAGATTTAACATACTGTTACTAAAATGCAGGCTGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCATGTCTTCAGATGTAAGACACTATTAAAAATAATGCATACATTCTATCATGTAATTTGCATACATATTGAACTTTCCCTTAACGTATTTATTAATGTATTATAACTTTGCCTTGCTCATGTTTGTTTGGCCTAACATCTTTTGTTTTATGTGCACAGATGTGATTGCATATACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGTAAGCAACTTGAATGAATTCATTGTGTCTTATTGCCTGATATGAAAAAGAAAGAGACGAAACATATAACTTGTTTGGAGTGATTTTCTAAGCGCTTAAAAACACTTTTGTGAGTACTTATACAAATTTGCAACGGTAGGCTTGTGATCATCCTATTAAACTGGACACAGAGGACCTCAACAATAGGATTCGTGAGAGAGTCCGAGGCGAGCTACAGTACTTGGATGGTGCATTCATCGTATCTTCAACAGTCATAAACAAGACCATTCGTACATCGTACGTACAAAGATAG

mRNA sequence

ATGTTTTCTTCCTTCTGCTCTGCCCTTTCATTTCTCTTTATCCCCAACTCCACTTCTCTCCCTCTCGATCATATCCACCAAGTATCTGAATGGTTCGAGGAACAACTCGAGGATGATCTCAAATGGTCCTTTGCCGTCAACAGGATACTTCATGCCGCAACTAGCGAGTTCCAAGATATAGTTCTGTTGGATACCAAGCGATTTGGGAAGGCTCTATTGCTTGATGGGAAGCTACAAAGTGCTGAAAAAGATGAATTCATTTATCATGAGTCCTTGGTTCATCCTGCTCTGTTACTGCATCATAACCCCAAAACAGTGTTTATAATGGGAGGTGGAGAAGGGTCTACTGCTAGAGAGACACTAAAGCATAAAAATCTTGAGGAAGTTGTCATGTGCGATATTGATAGGGATGTTGTCAATTTCTGCCGCACACATCTAAAAGAAAACCAAGATGCATTTCAGGACGAGAAGCTTCATATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACGACCAGAGAAGTTCGATGTAATCATTGGCGATTTATCAGATCCACATGAAGGAGGACCATGCAATCATCTGTACACTAAATCCTTCTATGAGGAAATGATTAAGCCCAAGCTCAATGACAATGGTATTTTTGTTACTCAGGCTGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCATGTCTTCAGATATGTGATTGCATATACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGCTTGTGATCATCCTATTAAACTGGACACAGAGGACCTCAACAATAGGATTCGTGAGAGAGTCCGAGGCGAGCTACAGTACTTGGATGGTGCATTCATCGTATCTTCAACAGTCATAAACAAGACCATTCGTACATCGTACGTACAAAGATAG

Coding sequence (CDS)

ATGTTTTCTTCCTTCTGCTCTGCCCTTTCATTTCTCTTTATCCCCAACTCCACTTCTCTCCCTCTCGATCATATCCACCAAGTATCTGAATGGTTCGAGGAACAACTCGAGGATGATCTCAAATGGTCCTTTGCCGTCAACAGGATACTTCATGCCGCAACTAGCGAGTTCCAAGATATAGTTCTGTTGGATACCAAGCGATTTGGGAAGGCTCTATTGCTTGATGGGAAGCTACAAAGTGCTGAAAAAGATGAATTCATTTATCATGAGTCCTTGGTTCATCCTGCTCTGTTACTGCATCATAACCCCAAAACAGTGTTTATAATGGGAGGTGGAGAAGGGTCTACTGCTAGAGAGACACTAAAGCATAAAAATCTTGAGGAAGTTGTCATGTGCGATATTGATAGGGATGTTGTCAATTTCTGCCGCACACATCTAAAAGAAAACCAAGATGCATTTCAGGACGAGAAGCTTCATATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACGACCAGAGAAGTTCGATGTAATCATTGGCGATTTATCAGATCCACATGAAGGAGGACCATGCAATCATCTGTACACTAAATCCTTCTATGAGGAAATGATTAAGCCCAAGCTCAATGACAATGGTATTTTTGTTACTCAGGCTGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCATGTCTTCAGATATGTGATTGCATATACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGCTTGTGATCATCCTATTAAACTGGACACAGAGGACCTCAACAATAGGATTCGTGAGAGAGTCCGAGGCGAGCTACAGTACTTGGATGGTGCATTCATCGTATCTTCAACAGTCATAAACAAGACCATTCGTACATCGTACGTACAAAGATAG

Protein sequence

MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTVINKTIRTSYVQR
Homology
BLAST of HG10006806 vs. NCBI nr
Match: XP_038890496.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 615.1 bits (1585), Expect = 3.2e-172
Identity = 294/308 (95.45%), Postives = 303/308 (98.38%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60
           MFSSFCSALS LFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI
Sbjct: 1   MFSSFCSALSVLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK++E+VVMCDIDRDVVNFCR HLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII
Sbjct: 121 LKHKDIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYEE+IKPKLNDNGIFVTQAGPAGILSH+VFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHEVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLACDHPI+LD EDLNNRIRERVRGEL YLDGA+IVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLACDHPIELDMEDLNNRIRERVRGELHYLDGAYIVSSTV 300

Query: 301 INKTIRTS 309
           +NKTIR S
Sbjct: 301 VNKTIRAS 308

BLAST of HG10006806 vs. NCBI nr
Match: XP_008459675.1 (PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa] >TYK10801.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa])

HSP 1 Score: 597.4 bits (1539), Expect = 6.9e-167
Identity = 284/307 (92.51%), Postives = 299/307 (97.39%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60
           MFSSFCSALS LFIPNS+SLPLDHIHQ+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQDI
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK++E+VVMCDIDRDVVNFCR HLKENQDAFQDEKLHIIFDDAKAGLEGR EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE++IKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHPI+LD EDLNN+I ERVRGEL+YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRT 308
           INKTIRT
Sbjct: 301 INKTIRT 307

BLAST of HG10006806 vs. NCBI nr
Match: XP_004141174.1 (thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical protein Csa_019446 [Cucumis sativus])

HSP 1 Score: 596.3 bits (1536), Expect = 1.5e-166
Identity = 283/307 (92.18%), Postives = 298/307 (97.07%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60
           MFSSFCSALS LF+PNS+SLPLDHI Q+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQDI
Sbjct: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120

Query: 121 LKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK++E+VVMCDIDRDVVNFCR HLKENQDAFQDE+LHIIFDDAKAGLEGRPEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE++IKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTV 300
           FRYVIAYTAHVPSYADSCGWVLA DHPIKLD EDLNN+IRERV+GEL YLDGAFIVSSTV
Sbjct: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300

Query: 301 INKTIRT 308
           INKTIRT
Sbjct: 301 INKTIRT 307

BLAST of HG10006806 vs. NCBI nr
Match: XP_022946737.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 551.2 bits (1419), Expect = 5.7e-153
Identity = 267/308 (86.69%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60
           M S  CSAL  L IP S S  + H   VSEWFEEQLEDDLKWSFAVNRILHAATS+FQDI
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPH---VSEWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEGSTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK++E+VVMCDIDRDVVNFCR HL ENQDAFQDEKLHIIFDDAK GLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLA DHP KLD EDL+ RIRERVRGEL+YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTS 309
           INKTIRT+
Sbjct: 301 INKTIRTA 305

BLAST of HG10006806 vs. NCBI nr
Match: KAG7030499.1 (Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 551.2 bits (1419), Expect = 5.7e-153
Identity = 266/308 (86.36%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60
           M S  CSAL  L IP S S  + H   VSEWFEEQLEDDLKWSFAVNRILHAATS+FQDI
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPH---VSEWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEGSTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK++E+VVMCDIDRDVVNFCR HL ENQDAFQDEKLHIIFDDAK GLEG+PEKFD+II
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLA DHP KLD EDL+ RIRERVRGEL+YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTS 309
           INKTIRT+
Sbjct: 301 INKTIRTA 305

BLAST of HG10006806 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.6e-110
Identity = 184/280 (65.71%), Postives = 227/280 (81.07%), Query Frame = 0

Query: 27  QVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEF 86
           Q   W+EE ++DDLKWSFA+N +LH  TSE+QDI LLDTKRFGK L++DGK+QSAE+DEF
Sbjct: 31  QDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEF 90

Query: 87  IYHESLVHPALLLHHNPKTVFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHL 146
           IYHE L+HPALL H NPKTVFIMGGGEGS ARE LKH  +E+VVMCDID++VV+FCR  L
Sbjct: 91  IYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFL 150

Query: 147 KENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEMIK 206
             N DAF ++KL ++  DAKA LE R EKFD+I+GDL+DP EGGPC  LYTKSFY+ ++K
Sbjct: 151 TVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNILK 210

Query: 207 PKLNDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD 266
           PKL+ NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+AD+ GWV+A D
Sbjct: 211 PKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMASD 270

Query: 267 HPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTVINKTI 306
           H   ++ ++++ RI ERV GEL YL+    VS+  +NKTI
Sbjct: 271 HEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTI 310

BLAST of HG10006806 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 8.8e-56
Identity = 112/265 (42.26%), Postives = 169/265 (63.77%), Query Frame = 0

Query: 45  AVNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPK 104
           A+ +++    + +Q  +L++  RFGKAL+LDGK+QS   DEFIYHE+LVHP LL  +NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 105 TVFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHLKE-NQDAFQDEKLHIIFD 164
            + I+GGGEG+T RE LKHK ++ V M DID  V++F + +L+E +Q AF + K  ++ +
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 165 DAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAG 224
           D    ++   EKFD ++ DL+DP +  P   LYTK FYEE+   +++  GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 225 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPIKLDTEDLNNRIRE 284
             S + FS IYNT+KHVF+ V A   +VP++    G+V A D  +P +   E++NNRI+E
Sbjct: 200 -FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIKE 259

Query: 285 RVRGELQYLDGAFIVSSTVINKTIR 307
           R+ G L++ DG   ++   I K IR
Sbjct: 260 RIDGSLRFYDGETHITMFSIPKHIR 280

BLAST of HG10006806 vs. ExPASy Swiss-Prot
Match: Q9YE02 (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=speE PE=3 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 5.3e-53
Identity = 117/282 (41.49%), Postives = 170/282 (60.28%), Query Frame = 0

Query: 31  WFEEQLEDDLKWSFAVNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHE 90
           WF E L  D      +  ++    S FQ+I ++     GK L+LDGK QS+E DEF+YHE
Sbjct: 15  WFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQSSESDEFMYHE 74

Query: 91  SLVHPALLLHHNPKTVFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHLKE-N 150
           +LVHPA++LH +P+ V I+GGGEG+T RE LKH+++E+ VM DID  VVN  R HL+E +
Sbjct: 75  ALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVVNVAREHLREWH 134

Query: 151 QDAFQDEKLHIIFDDAKAGLEGRPEK-FDVIIGDLSDPHEGGPCNHLYTKSFYEEMIKPK 210
           + AF D +  ++ DDA   +  + E  FDV+I DL DP E GP   LY++ +Y  M+K  
Sbjct: 135 RGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYSEEYY-RMVKDV 194

Query: 211 LNDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACDHP- 270
           +N  G+FVTQA     L+ +  + I NTV  VF++V +Y  +VPS+    G+V+A D   
Sbjct: 195 MNPGGVFVTQAVSISHLT-EYHAIIRNTVARVFKHVESYGVYVPSFDSMWGFVVASDDKD 254

Query: 271 --IKLDTEDLNNRIRERVRG-ELQYLDGAFIVSSTVINKTIR 307
             I  D      R+  +++G EL++LD A ++    I K  R
Sbjct: 255 PRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYR 294

BLAST of HG10006806 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.6e-52
Identity = 109/264 (41.29%), Postives = 161/264 (60.98%), Query Frame = 0

Query: 46  VNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKT 105
           + R++ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 106 VFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHLKE-NQDAFQDEKLHIIFDD 165
           V I+GGGEG+T RE LKH  +E+ VM DID ++V   + H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 166 AKAGLEGRPEKFDVIIGDLSDP-HEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAG 225
           A+A LE   E++DV+I DL+DP  E  P   LYT  FY  ++K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFY-RLVKAHLNPGGVMGMQAGMIL 200

Query: 226 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPIKLDTEDLNNRIRE 285
           +  H+V   ++ TV+  FRYV +Y  H+P +  + G++LA D   P       +  RIRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 286 RVRGELQYLDGAFIVSSTVINKTI 306
           R    L++L   ++ +  V+ K +
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDL 282

BLAST of HG10006806 vs. ExPASy Swiss-Prot
Match: Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.6e-52
Identity = 109/264 (41.29%), Postives = 161/264 (60.98%), Query Frame = 0

Query: 46  VNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKT 105
           + R++ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 106 VFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHLKE-NQDAFQDEKLHIIFDD 165
           V I+GGGEG+T RE LKH  +E+ VM DID ++V   + H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 166 AKAGLEGRPEKFDVIIGDLSDP-HEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAG 225
           A+A LE   E++DV+I DL+DP  E  P   LYT  FY  ++K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFY-RLVKAHLNPGGVMGMQAGMIL 200

Query: 226 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPIKLDTEDLNNRIRE 285
           +  H+V   ++ TV+  FRYV +Y  H+P +  + G++LA D   P       +  RIRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 286 RVRGELQYLDGAFIVSSTVINKTI 306
           R    L++L   ++ +  V+ K +
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDL 282

BLAST of HG10006806 vs. ExPASy TrEMBL
Match: A0A5A7UZV1 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001170 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.4e-167
Identity = 284/307 (92.51%), Postives = 299/307 (97.39%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60
           MFSSFCSALS LFIPNS+SLPLDHIHQ+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQDI
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK++E+VVMCDIDRDVVNFCR HLKENQDAFQDEKLHIIFDDAKAGLEGR EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE++IKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHPI+LD EDLNN+I ERVRGEL+YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRT 308
           INKTIRT
Sbjct: 301 INKTIRT 307

BLAST of HG10006806 vs. ExPASy TrEMBL
Match: A0A1S3CAR3 (thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.4e-167
Identity = 284/307 (92.51%), Postives = 299/307 (97.39%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60
           MFSSFCSALS LFIPNS+SLPLDHIHQ+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQDI
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK++E+VVMCDIDRDVVNFCR HLKENQDAFQDEKLHIIFDDAKAGLEGR EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE++IKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHPI+LD EDLNN+I ERVRGEL+YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRT 308
           INKTIRT
Sbjct: 301 INKTIRT 307

BLAST of HG10006806 vs. ExPASy TrEMBL
Match: A0A0A0LET5 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.5e-167
Identity = 283/307 (92.18%), Postives = 298/307 (97.07%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60
           MFSSFCSALS LF+PNS+SLPLDHI Q+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQDI
Sbjct: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120

Query: 121 LKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK++E+VVMCDIDRDVVNFCR HLKENQDAFQDE+LHIIFDDAKAGLEGRPEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE++IKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTV 300
           FRYVIAYTAHVPSYADSCGWVLA DHPIKLD EDLNN+IRERV+GEL YLDGAFIVSSTV
Sbjct: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300

Query: 301 INKTIRT 308
           INKTIRT
Sbjct: 301 INKTIRT 307

BLAST of HG10006806 vs. ExPASy TrEMBL
Match: A0A6J1G4J8 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450716 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.8e-153
Identity = 267/308 (86.69%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60
           M S  CSAL  L IP S S  + H   VSEWFEEQLEDDLKWSFAVNRILHAATS+FQDI
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVPH---VSEWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEGSTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           LKHK++E+VVMCDIDRDVVNFCR HL ENQDAFQDEKLHIIFDDAK GLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLA DHP KLD EDL+ RIRERVRGEL+YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTS 309
           INKTIRT+
Sbjct: 301 INKTIRTA 305

BLAST of HG10006806 vs. ExPASy TrEMBL
Match: A0A6J1KJ05 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111493662 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.5e-151
Identity = 266/310 (85.81%), Postives = 285/310 (91.94%), Query Frame = 0

Query: 1   MFSSFCSALSFLFIP--NSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQ 60
           M S  CSAL  L IP  NST++P     QVSEWFEEQLEDDLKWSFAV RILHAATS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----QVSEWFEEQLEDDLKWSFAVTRILHAATSKFQ 60

Query: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTAR 120
           DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDV 180
           ETLKHK++E+VVMCDIDRDVVNFCR HL ENQDAFQDEKLHIIF+DAK GLEG+PEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFNDAKVGLEGQPEKFDV 180

Query: 181 IIGDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVK 240
           II DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPIKLDTEDLNNRIRERVRGELQYLDGAFIVSS 300
           HVF+YVIAYTAH+PSYADS GWVLA DHP +LD EDL+ RIRERVRGEL+YLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLASDHPFELDMEDLDKRIRERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTS 309
           TVINKTIRT+
Sbjct: 301 TVINKTIRTA 305

BLAST of HG10006806 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.2e-111
Identity = 184/280 (65.71%), Postives = 227/280 (81.07%), Query Frame = 0

Query: 27  QVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEF 86
           Q   W+EE ++DDLKWSFA+N +LH  TSE+QDI LLDTKRFGK L++DGK+QSAE+DEF
Sbjct: 31  QDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEF 90

Query: 87  IYHESLVHPALLLHHNPKTVFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHL 146
           IYHE L+HPALL H NPKTVFIMGGGEGS ARE LKH  +E+VVMCDID++VV+FCR  L
Sbjct: 91  IYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFL 150

Query: 147 KENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEMIK 206
             N DAF ++KL ++  DAKA LE R EKFD+I+GDL+DP EGGPC  LYTKSFY+ ++K
Sbjct: 151 TVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNILK 210

Query: 207 PKLNDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD 266
           PKL+ NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+AD+ GWV+A D
Sbjct: 211 PKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMASD 270

Query: 267 HPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTVINKTI 306
           H   ++ ++++ RI ERV GEL YL+    VS+  +NKTI
Sbjct: 271 HEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTI 310

BLAST of HG10006806 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.2e-111
Identity = 184/280 (65.71%), Postives = 227/280 (81.07%), Query Frame = 0

Query: 27  QVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEF 86
           Q   W+EE ++DDLKWSFA+N +LH  TSE+QDI LLDTKRFGK L++DGK+QSAE+DEF
Sbjct: 24  QDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEF 83

Query: 87  IYHESLVHPALLLHHNPKTVFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHL 146
           IYHE L+HPALL H NPKTVFIMGGGEGS ARE LKH  +E+VVMCDID++VV+FCR  L
Sbjct: 84  IYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFL 143

Query: 147 KENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEMIK 206
             N DAF ++KL ++  DAKA LE R EKFD+I+GDL+DP EGGPC  LYTKSFY+ ++K
Sbjct: 144 TVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNILK 203

Query: 207 PKLNDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD 266
           PKL+ NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+AD+ GWV+A D
Sbjct: 204 PKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMASD 263

Query: 267 HPIKLDTEDLNNRIRERVRGELQYLDGAFIVSSTVINKTI 306
           H   ++ ++++ RI ERV GEL YL+    VS+  +NKTI
Sbjct: 264 HEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTI 303

BLAST of HG10006806 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 129.4 bits (324), Expect = 5.0e-30
Identity = 69/224 (30.80%), Postives = 119/224 (53.12%), Query Frame = 0

Query: 43  SFAVNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHN 102
           S  V ++L    S++QD+++  +  +GK L+LDG +Q  E+DE  Y E + H  L    N
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTVFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIF 162
           PK V ++GGG+G   RE  +H ++E++ MC+ID+ VV+  +    +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 DDAKAGLEGRPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGP 222
            D  A L+   E  +D +I D SDP   GP   L+ K F++ + +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQAES 284

Query: 223 AGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCG-WVLA 264
              L   +   I +  + +F+  + Y    VP+Y    G W+ A
Sbjct: 285 LW-LHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYA 324

BLAST of HG10006806 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 128.3 bits (321), Expect = 1.1e-29
Identity = 69/224 (30.80%), Postives = 116/224 (51.79%), Query Frame = 0

Query: 43  SFAVNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHN 102
           S  V +IL    S++QD+++  +  +GK L+LDG +Q  E+DE  Y E + H  L    N
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 103 PKTVFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIF 162
           PK V ++GGG+G   RE  +H ++E++ +C+ID+ VV+  + +       ++D ++++I 
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 163 DDAKAGLEGRPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGP 222
            D  A L+   E  +D +I D SDP   GP   L+ K F+E  +   L   G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFES-VNRALRPGGVVCTQAES 244

Query: 223 AGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCGWVLAC 265
              L   +   I +  + +F+  + Y    VP+Y       + C
Sbjct: 245 LW-LHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLC 284

BLAST of HG10006806 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-29
Identity = 67/224 (29.91%), Postives = 117/224 (52.23%), Query Frame = 0

Query: 43  SFAVNRILHAATSEFQDIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHN 102
           S  V ++L    S++QD+++  +  +GK L+LDG +Q  E+DE  Y E + H  L    N
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTVFIMGGGEGSTARETLKHKNLEEVVMCDIDRDVVNFCRTHLKENQDAFQDEKLHIIF 162
           PK V ++GGG+G   RE  +H ++E++ MC+ID+ VV+  +    +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 DDAKAGLEGRPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEEMIKPKLNDNGIFVTQAGP 222
            D  A L+   E  +D +I D SDP   GP   L+ K F++ + +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQAES 284

Query: 223 AGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCGWVLAC 265
              L   +   I +  + +F+  + Y    VP+Y       + C
Sbjct: 285 LW-LHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLC 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890496.13.2e-17295.45thermospermine synthase ACAULIS5-like [Benincasa hispida][more]
XP_008459675.16.9e-16792.51PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 th... [more]
XP_004141174.11.5e-16692.18thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical prot... [more]
XP_022946737.15.7e-15386.69thermospermine synthase ACAULIS5-like [Cucurbita moschata][more]
KAG7030499.15.7e-15386.36Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9S7X61.6e-11065.71Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV... [more]
Q975S58.8e-5642.26Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ... [more]
Q9YE025.3e-5341.49Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 1... [more]
Q72K552.6e-5241.29Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ... [more]
Q5SK282.6e-5241.29Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS... [more]
Match NameE-valueIdentityDescription
A0A5A7UZV13.4e-16792.51Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CAR33.4e-16792.51thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE... [more]
A0A0A0LET57.5e-16792.18PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 S... [more]
A0A6J1G4J82.8e-15386.69thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450... [more]
A0A6J1KJ051.5e-15185.81thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149366... [more]
Match NameE-valueIdentityDescription
AT5G19530.11.2e-11165.71S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G19530.21.2e-11165.71S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G23820.25.0e-3030.80spermidine synthase 1 [more]
AT1G70310.11.1e-2930.80spermidine synthase 2 [more]
AT1G23820.11.5e-2929.91spermidine synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 85..257
e-value: 2.9E-38
score: 131.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 82..312
e-value: 1.1E-70
score: 240.0
NoneNo IPR availablePANTHERPTHR43317:SF6THERMOSPERMINE SYNTHASE ACAULIS5-LIKEcoord: 3..310
NoneNo IPR availablePANTHERPTHR43317THERMOSPERMINE SYNTHASE ACAULIS5coord: 3..310
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 110..218
e-value: 1.27533E-6
score: 44.3431
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 28..81
e-value: 1.8E-16
score: 61.9
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 31..82
e-value: 4.0E-13
score: 49.1
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 29..265
score: 89.318497
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 30..311
score: 29.998821
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 28..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10006806.1HG10006806.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006596 polyamine biosynthetic process
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0010487 thermospermine synthase activity
molecular_function GO:0003824 catalytic activity