HG10006601 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10006601
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionchitotriosidase-1-like
LocationChr07: 20248934 .. 20250007 (-)
RNA-Seq ExpressionHG10006601
SyntenyHG10006601
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAATCCCCAAACCACAAAGTTTGTGAATGCTGGTTACTGGCTTGCAAGCACAAATGATAAACTTCCACCAGCAAATATCAATCCTAACCTTTTCACCCATTTGTTTGTTGGTTATGCTGGTGTTGATGCTCAAACTTACAAAGTCACTATAGATCCTTCACAAGATGTTTTAATCAATCAGTTTTCATCAACTGTCAGACTACGAAATCCCGACGTCAAAACTCTCTTGTCCATCGCAGGATTTAGCGAAAGATTCGCTAAAATCGCTACCGACGCTTCGAATCGTGACGTTTTTATCAAATCCTCCATTGATGTAGCAAGAGCAGGTAAGTTTGATGGCCTTGATCTTCAATGGCTATACCCATCTTCTCATGATGACATGAACAATTTTGAAACTTTGGTTAGTGTTTGGCATAAAGCTATTGTGGAAGATGCTATTAATTTGAGTCTTCCAAGGTTACTTCTTGTTGCTACTGTCTCTAATGTACCTTATCTTCAACACAACATTCAGTATCCAGTTGATGCTATTAAACAAAATTTGGATTGGGTGAATATACTTTCTTATGATTTTTATACACCAATTTCATCAAGTAAAGCTACTAGCCCTTCTTCAGCATTCAACAACCCAAAGGCTAATGCTTTATCTGCAAATTTTGGTATTAAGACTTGGATACAAAGTCTTAATAATCTTCCTCATAAGAAAATTGTGTTTGGAATTCCTTTCCATGGTTGGGCTTGGAAACTTGTCGACTCTCACCGGCACGACGTGTTTTCGAAAACCGACGGAGCGGCGAAAGGAGACAATATTTTAGAAAATGGACAAATTTATTACTCTGATGTGAAGAAATTTATAGAGGAGAATCATGGTGGAGTTGAGAACAATGATAAAAGGTTTCTGATTGCTTACATTTATTATGACACAACATGGATTGCATATGAAAGTGAATACACAATTGCAGATAAGATTTCATTGGCAAAGTTAAATAACGTTCTTGGTTACTTTGCTTACAACATTGCAGCTGATGATGATGTTAATACTCTTGCTAATGCTGCCAAATCCAATTGGTGA

mRNA sequence

ATGGAAAATCCCCAAACCACAAAGTTTGTGAATGCTGGTTACTGGCTTGCAAGCACAAATGATAAACTTCCACCAGCAAATATCAATCCTAACCTTTTCACCCATTTGTTTGTTGGTTATGCTGGTGTTGATGCTCAAACTTACAAAGTCACTATAGATCCTTCACAAGATGTTTTAATCAATCAGTTTTCATCAACTGTCAGACTACGAAATCCCGACGTCAAAACTCTCTTGTCCATCGCAGGATTTAGCGAAAGATTCGCTAAAATCGCTACCGACGCTTCGAATCGTGACGTTTTTATCAAATCCTCCATTGATGTAGCAAGAGCAGGTAAGTTTGATGGCCTTGATCTTCAATGGCTATACCCATCTTCTCATGATGACATGAACAATTTTGAAACTTTGGTTAGTGTTTGGCATAAAGCTATTGTGGAAGATGCTATTAATTTGAGTCTTCCAAGGTTACTTCTTGTTGCTACTGTCTCTAATGTACCTTATCTTCAACACAACATTCAGTATCCAGTTGATGCTATTAAACAAAATTTGGATTGGGTGAATATACTTTCTTATGATTTTTATACACCAATTTCATCAAGTAAAGCTACTAGCCCTTCTTCAGCATTCAACAACCCAAAGGCTAATGCTTTATCTGCAAATTTTGGTATTAAGACTTGGATACAAAGTCTTAATAATCTTCCTCATAAGAAAATTGTGTTTGGAATTCCTTTCCATGGTTGGGCTTGGAAACTTGTCGACTCTCACCGGCACGACGTGTTTTCGAAAACCGACGGAGCGGCGAAAGGAGACAATATTTTAGAAAATGGACAAATTTATTACTCTGATGTGAAGAAATTTATAGAGGAGAATCATGGTGGAGTTGAGAACAATGATAAAAGGTTTCTGATTGCTTACATTTATTATGACACAACATGGATTGCATATGAAAGTGAATACACAATTGCAGATAAGATTTCATTGGCAAAGTTAAATAACGTTCTTGGTTACTTTGCTTACAACATTGCAGCTGATGATGATGTTAATACTCTTGCTAATGCTGCCAAATCCAATTGGTGA

Coding sequence (CDS)

ATGGAAAATCCCCAAACCACAAAGTTTGTGAATGCTGGTTACTGGCTTGCAAGCACAAATGATAAACTTCCACCAGCAAATATCAATCCTAACCTTTTCACCCATTTGTTTGTTGGTTATGCTGGTGTTGATGCTCAAACTTACAAAGTCACTATAGATCCTTCACAAGATGTTTTAATCAATCAGTTTTCATCAACTGTCAGACTACGAAATCCCGACGTCAAAACTCTCTTGTCCATCGCAGGATTTAGCGAAAGATTCGCTAAAATCGCTACCGACGCTTCGAATCGTGACGTTTTTATCAAATCCTCCATTGATGTAGCAAGAGCAGGTAAGTTTGATGGCCTTGATCTTCAATGGCTATACCCATCTTCTCATGATGACATGAACAATTTTGAAACTTTGGTTAGTGTTTGGCATAAAGCTATTGTGGAAGATGCTATTAATTTGAGTCTTCCAAGGTTACTTCTTGTTGCTACTGTCTCTAATGTACCTTATCTTCAACACAACATTCAGTATCCAGTTGATGCTATTAAACAAAATTTGGATTGGGTGAATATACTTTCTTATGATTTTTATACACCAATTTCATCAAGTAAAGCTACTAGCCCTTCTTCAGCATTCAACAACCCAAAGGCTAATGCTTTATCTGCAAATTTTGGTATTAAGACTTGGATACAAAGTCTTAATAATCTTCCTCATAAGAAAATTGTGTTTGGAATTCCTTTCCATGGTTGGGCTTGGAAACTTGTCGACTCTCACCGGCACGACGTGTTTTCGAAAACCGACGGAGCGGCGAAAGGAGACAATATTTTAGAAAATGGACAAATTTATTACTCTGATGTGAAGAAATTTATAGAGGAGAATCATGGTGGAGTTGAGAACAATGATAAAAGGTTTCTGATTGCTTACATTTATTATGACACAACATGGATTGCATATGAAAGTGAATACACAATTGCAGATAAGATTTCATTGGCAAAGTTAAATAACGTTCTTGGTTACTTTGCTTACAACATTGCAGCTGATGATGATGTTAATACTCTTGCTAATGCTGCCAAATCCAATTGGTGA

Protein sequence

MENPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSSTVRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQNLDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGIPFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFLIAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW
Homology
BLAST of HG10006601 vs. NCBI nr
Match: KAG6599362.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1 Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 536.2 bits (1380), Expect = 2.2e-148
Identity = 256/356 (71.91%), Postives = 301/356 (84.55%), Query Frame = 0

Query: 2   ENPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLIN 61
           E    T+FVNAGYWLAST+D+LP ANI+  LFTHLF GYA +++ TY+VTIDPSQ +LI+
Sbjct: 3   EFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILID 62

Query: 62  QFSSTVRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWL 121
           QFSSTV+L NP+VKTLLSIA  +  FA +A D +NRD FIKSSI  AR G FDGLDLQWL
Sbjct: 63  QFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWL 122

Query: 122 YPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQN 181
           YPSS D M+NF+TL+  WH+A+V+DA +LSLP+LLLVATVSN+PYLQ NIQYP DAI Q 
Sbjct: 123 YPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQK 182

Query: 182 LDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGI 241
           LDWVN+LSYDFYTP SS  AT PSSAF+NPKANALSANFGI++W QSL+NLPHKKIVFGI
Sbjct: 183 LDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGI 242

Query: 242 PFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFL 301
           PFHGWAWKL +SH+H +F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L
Sbjct: 243 PFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 302

Query: 302 IAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           +AY YY+TTWI YESEYTIADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 303 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of HG10006601 vs. NCBI nr
Match: KAG7030350.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 535.8 bits (1379), Expect = 2.8e-148
Identity = 255/356 (71.63%), Postives = 301/356 (84.55%), Query Frame = 0

Query: 2   ENPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLIN 61
           E    T+FVNAGYWLAST+D+LP ANI+  LFTHLF GYA +++ TY+VTIDPS  +LI+
Sbjct: 3   EFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILID 62

Query: 62  QFSSTVRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWL 121
           QFSSTV+L NP+VKTLLSIA  +  FA +A D +NRD FIKSSI  AR G FDGLDLQWL
Sbjct: 63  QFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWL 122

Query: 122 YPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQN 181
           YPSS D M+NF+TL+  WH+A+V+DA +LSLP+LLLVATVSN+PYLQ NIQYP DAI Q 
Sbjct: 123 YPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQK 182

Query: 182 LDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGI 241
           LDWVN+LSYDFYTP SS  AT PSSAF+NPKANALSANFGI++W+QSL+NLPHKKIVFGI
Sbjct: 183 LDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGI 242

Query: 242 PFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFL 301
           PFHGWAWKL +SH+H +F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L
Sbjct: 243 PFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 302

Query: 302 IAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           +AY YY+TTWI YESEYTIADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 303 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of HG10006601 vs. NCBI nr
Match: XP_022946077.1 (acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mammalian chitinase-like [Cucurbita moschata])

HSP 1 Score: 534.6 bits (1376), Expect = 6.3e-148
Identity = 255/356 (71.63%), Postives = 301/356 (84.55%), Query Frame = 0

Query: 2   ENPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLIN 61
           E    T+FVNAGYWLAST+D+LP ANI+  LFTHLF GYA +++ TY+VT+DPSQ +LI+
Sbjct: 3   EFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILID 62

Query: 62  QFSSTVRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWL 121
           QFSSTV+L NP+VKTLLSIA  +  FA +A D +NRD FIKSSI  AR G FDGLDLQWL
Sbjct: 63  QFSSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWL 122

Query: 122 YPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQN 181
           YPSS D M+NF+TL+  WH+A+V+DA +LSLP+LLLVATVSN+PYLQ NIQYP DAI Q 
Sbjct: 123 YPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQK 182

Query: 182 LDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGI 241
           LDWVN+LSYDFYTP SS  AT PSSAF+NPKANALSANFGI++W QSL+NLPHKKIVFGI
Sbjct: 183 LDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGI 242

Query: 242 PFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFL 301
           PFHGWAWKL +SH+H +F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L
Sbjct: 243 PFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 302

Query: 302 IAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           +AY YY+TTWI YESEYTIADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 303 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of HG10006601 vs. NCBI nr
Match: KAG6599434.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 532.7 bits (1371), Expect = 2.4e-147
Identity = 254/356 (71.35%), Postives = 300/356 (84.27%), Query Frame = 0

Query: 2   ENPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLIN 61
           E    T+FVNAGYWLAST+D+LP ANI+  LFTHLF GYA +++ TY+VTIDPS  +LI+
Sbjct: 3   EFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILID 62

Query: 62  QFSSTVRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWL 121
           QFSSTV+L NP+VKTLLSIA  +  FA +A D +NRD FIKSSI  AR G FDGLDLQWL
Sbjct: 63  QFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWL 122

Query: 122 YPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQN 181
           YPSS D M+NF+TL+  WH+A+V+DA +LSLP+LLLVATVSN+PYL+ NIQYP DAI Q 
Sbjct: 123 YPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLKSNIQYPADAINQK 182

Query: 182 LDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGI 241
           LDWVN+LSYDFYTP SS  AT PSSAF+NPKANALSANFGI++W QSL+NLPHKKIVFGI
Sbjct: 183 LDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGI 242

Query: 242 PFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFL 301
           PFHGWAWKL +SH+H +F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L
Sbjct: 243 PFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 302

Query: 302 IAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           +AY YY+TTWI YESEYTIADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 303 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of HG10006601 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 532.3 bits (1370), Expect = 3.1e-147
Identity = 252/351 (71.79%), Postives = 299/351 (85.19%), Query Frame = 0

Query: 7   TKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSST 66
           T+FVNAGYWLAST+D+LP ANI+  LFTHLF GYA +++ TY+VTIDPSQ +L+NQFS T
Sbjct: 14  TEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSPT 73

Query: 67  VRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSH 126
           VRL NPDVKTLLSIA  +  FA +A D +NRD FIKSSI  AR G FDGLDLQWLYPSS 
Sbjct: 74  VRLSNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQ 133

Query: 127 DDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQNLDWVN 186
           ++M NF+TL+  WH+A+V+DA +LSLP+LLLVATVSN+PYLQ NIQYP DAI Q LDWVN
Sbjct: 134 EEMGNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVN 193

Query: 187 ILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGIPFHGW 246
           +LSYDFYTP S+  AT PSSAF+NPKANALSANFGI++W QSL+NLPHKKIVFGIPFHGW
Sbjct: 194 LLSYDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGW 253

Query: 247 AWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFLIAYIY 306
           AWKL +SH+H +F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L+AY Y
Sbjct: 254 AWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYTY 313

Query: 307 YDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           ++TTWI YES+YTIADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 314 FETTWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 364

BLAST of HG10006601 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.1e-65
Identity = 141/353 (39.94%), Postives = 209/353 (59.21%), Query Frame = 0

Query: 10  VNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSSTVRL 69
           V A YW  ++  + P  +I+ +LFTHLF  +A +++QT +VT+  +     + F+ TV+ 
Sbjct: 28  VKASYWFPAS--EFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 70  RNPDVKTLLSIAG---FSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSH 129
           RNP VKTLLSI G       +A +A++ ++R  FI SSI VAR+  F GLDL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 130 DDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATV--SNVPYLQHNIQYPVDAIKQNLDW 189
            +M NF TL+  W  A+V +A +   PRLLL A V  SN  Y   ++ YPV A+  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 190 VNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGIPFH 249
           VN+++YDFY P   S+ T P +A  +P     S + G ++WIQ+   LP KK V G P++
Sbjct: 208 VNLMAYDFYGP-GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYY 267

Query: 250 GWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFLIAY 309
           G+AW+L +++ H  ++ T GAA    I  +G I Y  ++KFI +N G     +   +  Y
Sbjct: 268 GYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDN-GATTVYNSTVVGDY 327

Query: 310 IYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
            Y  T WI Y+   +I  K+  AK   +LGYF++++ ADD+ + L+ AA   W
Sbjct: 328 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of HG10006601 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.2e-47
Identity = 121/350 (34.57%), Postives = 182/350 (52.00%), Query Frame = 0

Query: 14  YWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSSTVRLRNPD 73
           YWL   N   PP  ++ +LFTH+F  +   +  TYK+ I  SQ + +N F+ T + ++P 
Sbjct: 39  YWL--ENPFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKTKSPP 98

Query: 74  VKTLLSIAGF---SERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSHDDMN 133
             TL SI G    S   A IA+D      FI S+IDVAR   FDG+DL W +P +  +MN
Sbjct: 99  AATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNTKEMN 158

Query: 134 NFETLVSVWHKAIVEDAINLSLPRLLLVATV---SNVPYLQHNIQYPVDAIKQNLDWVNI 193
           +   ++  W KAI ++      P LLL A V    N         YPV++I +NLDWVN+
Sbjct: 159 DLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLDWVNV 218

Query: 194 LSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGIPFHGWA 253
           +SY+   P  S+K  +PS  F +PK+N +S   G+ +WI S   +  +K+V G+P +G +
Sbjct: 219 MSYELRGP-RSNKTGAPSGTF-DPKSN-VSVVSGLLSWIHS--GVVPEKLVMGMPLYGKS 278

Query: 254 WKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFLIAYIYY 313
           WKL D + H + + + G+  G     NG + Y  V  F  +    VE  D      Y Y 
Sbjct: 279 WKLRDPNVHGIGAPSVGSGPG----VNGLMAYFQVLDFNRQKSAKVE-YDVDTASVYSYS 338

Query: 314 DTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
            +TWI Y++ +T++ K+  A+   + GYF +    D     +A  A   W
Sbjct: 339 GSTWIGYDNPFTVSIKVGFAQALKLRGYFFWVAGLDTLDWKIATQASKAW 376

BLAST of HG10006601 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 184.5 bits (467), Expect = 2.1e-45
Identity = 118/363 (32.51%), Postives = 185/363 (50.96%), Query Frame = 0

Query: 3   NPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLI-- 62
           +P +T  V   YW+   N   PP++I+ +LFTH+F  +   +  TYK+  +      +  
Sbjct: 30  SPSSTTRVKGIYWI--ENPLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVAT 89

Query: 63  --NQFSSTVRLRNPDVKTLLSIAGF---SERFAKIATDASNRDVFIKSSIDVARAGKFDG 122
               F++T + + P + TLLSI G    S  FA IA+D + R  FI S+I VAR   FDG
Sbjct: 90  SLTTFTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDG 149

Query: 123 LDLQWLYPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATV---SNVPYLQHNIQ 182
           +D  W +P++  +MN+   L+  W +AI ++  + S P LLL A V    N         
Sbjct: 150 IDFDWEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRM 209

Query: 183 YPVDAIKQNLDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNL 242
           YPVD+I +NLDWVN++SYD     S S  T   S   + K+N +S   G+ +WI+    +
Sbjct: 210 YPVDSINKNLDWVNVMSYDLRG--SGSNVTGAPSGMFDSKSN-VSVVSGLFSWIR--GGV 269

Query: 243 PHKKIVFGIPFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGG 302
             +KIV G+P +G +WKL D + H + +   G   G   ++ G  Y+  V     +  G 
Sbjct: 270 APEKIVMGMPLYGKSWKLQDPNVHGIGAPNVGPGPG---VDGGMAYFQVVD--FNKQMGA 329

Query: 303 VENNDKRFLIAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANA 356
               DK     Y Y  +TWI Y+  +T++ K+  A+   + GYF +    D     ++ A
Sbjct: 330 KVVYDKETGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFWAAGYDTSDWKVSTA 380

BLAST of HG10006601 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.9e-42
Identity = 116/356 (32.58%), Postives = 191/356 (53.65%), Query Frame = 0

Query: 10  VNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSSTVRL 69
           V + YW A   D   P+ I+ N FTH+ + +   +  ++K+ I  S       F   +R 
Sbjct: 40  VRSAYWPA--GDDFSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 70  RNPDVKTLLSIAG---FSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSH 129
           R+P VKTLLSI G    S  F++IA+   NR++FI S+I+VAR  +FDG+DL W +P + 
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 130 DDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATV---SNVPYL-QHNIQ-YPVDAIKQN 189
            DM N   L   W+ A+  +A     PRLLL + V   S V  + +H  + YP  AI + 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 190 LDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGI 249
           LDW + + +D++    ++  T  ++A  + K+  +S NFG+ +WI+S   +  +K+V G+
Sbjct: 220 LDWASPMCFDYHGTWDNN--TDFNAALYDSKSE-ISTNFGLHSWIKS--GVRPEKLVMGL 279

Query: 250 PFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFL 309
             +G AW+L D + + V ++  G A       +G + Y+++ KF +++   V   DK  +
Sbjct: 280 ALYGRAWELKDPNVNGVGAEAVGPATD----TDGSMNYNEILKFNKQSGANVV-YDKVAI 339

Query: 310 IAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
             Y Y  TTWI Y+   +I  K+  AK   + GYF + +  D D  +++  A + W
Sbjct: 340 SFYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKDW-SISKQASNAW 382

BLAST of HG10006601 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 167.2 bits (422), Expect = 3.5e-40
Identity = 113/363 (31.13%), Postives = 189/363 (52.07%), Query Frame = 0

Query: 2   ENPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLIN 61
           + P +   V A YW A ++     ++I+ N FTH++  +     +++ + I  S      
Sbjct: 47  QQPPSKGGVRAAYWPAWSD--FSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAP 106

Query: 62  QFSSTVRLRNPDVKTLLSIAGFSER---FAKIATDASNRDVFIKSSIDVARAGKFDGLDL 121
           ++     +R P V TLLSI G       F+++A+   NR  FI S+I VAR  +F+GLDL
Sbjct: 107 KYDGIHNIR-PRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDL 166

Query: 122 QWLYPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATV---SNVPYLQHNIQ-YP 181
            W +P    DM+N   L+  W+KA+V +A      RLLL + V   S +  + +  + YP
Sbjct: 167 DWEWPGDEKDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYP 226

Query: 182 VDAIKQNLDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPH 241
           V AI++ LDW + + +D+    + +  T  ++A  +P +N +S  +GI +WI S   +P 
Sbjct: 227 VRAIRKYLDWASPMCFDYNG--AWANETGFNAALYDPNSN-ISTKYGIGSWIGS--GVPA 286

Query: 242 KKIVFGIPFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVE 301
           +K+V G+P +G AW+L D + H V +K  G A    +  +G + Y ++  F  ++ G   
Sbjct: 287 EKLVMGLPLYGRAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVF-NKDTGAKV 346

Query: 302 NNDKRFLIAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAK 358
             D+  +  Y Y  TTWI Y+   +I  K+  A+   + GYF + I  D D  T++  A 
Sbjct: 347 VYDEVAVSFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDW-TISKQAS 395

BLAST of HG10006601 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 3.1e-148
Identity = 255/356 (71.63%), Postives = 301/356 (84.55%), Query Frame = 0

Query: 2   ENPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLIN 61
           E    T+FVNAGYWLAST+D+LP ANI+  LFTHLF GYA +++ TY+VT+DPSQ +LI+
Sbjct: 3   EFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILID 62

Query: 62  QFSSTVRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWL 121
           QFSSTV+L NP+VKTLLSIA  +  FA +A D +NRD FIKSSI  AR G FDGLDLQWL
Sbjct: 63  QFSSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWL 122

Query: 122 YPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQN 181
           YPSS D M+NF+TL+  WH+A+V+DA +LSLP+LLLVATVSN+PYLQ NIQYP DAI Q 
Sbjct: 123 YPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQK 182

Query: 182 LDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGI 241
           LDWVN+LSYDFYTP SS  AT PSSAF+NPKANALSANFGI++W QSL+NLPHKKIVFGI
Sbjct: 183 LDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGI 242

Query: 242 PFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFL 301
           PFHGWAWKL +SH+H +F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L
Sbjct: 243 PFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 302

Query: 302 IAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           +AY YY+TTWI YESEYTIADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 303 VAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of HG10006601 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 2.2e-146
Identity = 252/356 (70.79%), Postives = 298/356 (83.71%), Query Frame = 0

Query: 2   ENPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLIN 61
           E    T+FVNAGYWLAST+  LP ANI+  LFTHLF GYA +++ TY+VT+DPSQ +LI+
Sbjct: 3   EFANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILID 62

Query: 62  QFSSTVRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWL 121
           QFSSTV+L NP VKTLLSIAG +  FA +A D +NRD FIKSSI  AR G FDGLDLQWL
Sbjct: 63  QFSSTVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWL 122

Query: 122 YPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQN 181
           YPSS D M NF+TL+  WH+A+V+DA ++SLP+LLLVATVSN+PYLQ+NIQYP DAI QN
Sbjct: 123 YPSSQDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQN 182

Query: 182 LDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGI 241
           LDWVN+LSYDFYTP S   AT PSSAF+NPKANALSANFGI++W QSLNNL HKKIVFGI
Sbjct: 183 LDWVNLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGI 242

Query: 242 PFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFL 301
           PFHGWAWKL +SH+H +F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L
Sbjct: 243 PFHGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 302

Query: 302 IAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           +AY YY+TTWI +ESEYT+ADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 303 VAYAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of HG10006601 vs. ExPASy TrEMBL
Match: A0A6J1G4H5 (chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.9e-146
Identity = 253/351 (72.08%), Postives = 296/351 (84.33%), Query Frame = 0

Query: 7   TKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSST 66
           TKFVNAGYWLASTND+L  ANI+  LFTHLFVGYA +D +T++VT+DPSQ +LINQFSST
Sbjct: 8   TKFVNAGYWLASTNDRLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSST 67

Query: 67  VRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSH 126
           V+L NPDVKTLLSIA  +  FA +A   +NRD FIKSSI  AR G FDGLDLQWLYPSS 
Sbjct: 68  VKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSSQ 127

Query: 127 DDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQNLDWVN 186
            +M NF+TL+  WH+A+V+DA  +SLP+LLLVATVSN+PYLQ N++YP DAI Q LDWVN
Sbjct: 128 YEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLDWVN 187

Query: 187 ILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGIPFHGW 246
           +LSYDFYTP SS  AT PSSAF+NPKANALSANFGI++W QSL+NLPHKKIVFGIPFHGW
Sbjct: 188 LLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGW 247

Query: 247 AWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFLIAYIY 306
           AWKL +SH+H  F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L+AY Y
Sbjct: 248 AWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAY 307

Query: 307 YDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           Y+TTWI YESEYTIADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 308 YETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of HG10006601 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 8.3e-146
Identity = 251/356 (70.51%), Postives = 298/356 (83.71%), Query Frame = 0

Query: 2   ENPQTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLIN 61
           E    T+FVNAGYWLAST+  LP ANI+  LFTHLF GYA +++ TY+VT+DPSQ +LI+
Sbjct: 3   EFANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILID 62

Query: 62  QFSSTVRLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWL 121
           QFSSTV+L NPDVKTLLSIAG +  FA +A D +NRD FIKSSI  AR G FDGLDLQWL
Sbjct: 63  QFSSTVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWL 122

Query: 122 YPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQN 181
           YPSS D M NF+TL+  WH+A+V+DA ++SLP+LLLVATVSN+PYLQ+NIQYP DAI Q 
Sbjct: 123 YPSSQDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQK 182

Query: 182 LDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGI 241
           LDWVN+LSYDFYTP S   AT PSSAF+NPKANALSANFGI++W QSL+NL HKKIVFGI
Sbjct: 183 LDWVNLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGI 242

Query: 242 PFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFL 301
           PFHGWAWKL +SH+H +F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L
Sbjct: 243 PFHGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 302

Query: 302 IAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           +AY YY+TTWI +ESEYT+ADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 303 VAYAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of HG10006601 vs. ExPASy TrEMBL
Match: A0A6J1G4J9 (acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.8e-144
Identity = 249/350 (71.14%), Postives = 293/350 (83.71%), Query Frame = 0

Query: 8   KFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSSTV 67
           +FVNAGYWLA T+D L  ANI+  LFTHLFVGYA +D +T++VT+DPSQ +LINQFSSTV
Sbjct: 3   EFVNAGYWLAGTDDTLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSSTV 62

Query: 68  RLRNPDVKTLLSIAGFSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSHD 127
           +L NPDVKTLLSIA  +  FA +A   +NRD FIKSSI  AR G FDGLDLQWLYPSS  
Sbjct: 63  KLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSSQY 122

Query: 128 DMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQNLDWVNI 187
           +M NF+TL+  WH+A+V+DA  +SLP+LLLVATVSN+PYLQ N++YP DAI Q LDWVN+
Sbjct: 123 EMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLDWVNL 182

Query: 188 LSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGIPFHGWA 247
           LSYDFYTP SS  AT PSSAF+NPKANALSANFGI++W QSL+NLPHKKIVFGIPFHGWA
Sbjct: 183 LSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWA 242

Query: 248 WKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFLIAYIYY 307
           WKL +SH+H  F+K DGAA G+ I  +GQI+YSDV+KFIE+N  GVE+ DK +L+AY YY
Sbjct: 243 WKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYY 302

Query: 308 DTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
           +TTWI YESEYTIADKI+LAK + VLGYFA+NIAADD  NTLANAAKSNW
Sbjct: 303 ETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 352

BLAST of HG10006601 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 250.0 bits (637), Expect = 2.9e-66
Identity = 141/353 (39.94%), Postives = 209/353 (59.21%), Query Frame = 0

Query: 10  VNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSSTVRL 69
           V A YW  ++  + P  +I+ +LFTHLF  +A +++QT +VT+  +     + F+ TV+ 
Sbjct: 28  VKASYWFPAS--EFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 70  RNPDVKTLLSIAG---FSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSH 129
           RNP VKTLLSI G       +A +A++ ++R  FI SSI VAR+  F GLDL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 130 DDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATV--SNVPYLQHNIQYPVDAIKQNLDW 189
            +M NF TL+  W  A+V +A +   PRLLL A V  SN  Y   ++ YPV A+  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 190 VNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGIPFH 249
           VN+++YDFY P   S+ T P +A  +P     S + G ++WIQ+   LP KK V G P++
Sbjct: 208 VNLMAYDFYGP-GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYY 267

Query: 250 GWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFLIAY 309
           G+AW+L +++ H  ++ T GAA    I  +G I Y  ++KFI +N G     +   +  Y
Sbjct: 268 GYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDN-GATTVYNSTVVGDY 327

Query: 310 IYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANAAKSNW 358
            Y  T WI Y+   +I  K+  AK   +LGYF++++ ADD+ + L+ AA   W
Sbjct: 328 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of HG10006601 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-63
Identity = 133/342 (38.89%), Postives = 192/342 (56.14%), Query Frame = 0

Query: 5   QTTKFVNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFS 64
           +T   V A YW+    +  P  NI+   FTHLF  +A VD+ T++VTI  +    ++ F+
Sbjct: 9   RTNSIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFT 68

Query: 65  STVRLRNPDVKTLLSIAGFSER---FAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWL 124
            TV+ +N DV+TLLSI G        A +A+++ NR  FI SSID+AR   F GLDL W 
Sbjct: 69  HTVKDKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWE 128

Query: 125 YPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQN 184
           YPS+  +M NF  LV  W  A+VE++   +   LLL A V   P   +  +YPV AI  N
Sbjct: 129 YPSNDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPD-YYGEEYPVQAIADN 188

Query: 185 LDWVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGI 244
           LD+VNI++YDFY P  S     P++ F+       S + G+  W+++   LP KK V G 
Sbjct: 189 LDFVNIMAYDFYGPGWSPVTGPPAALFDPSNPAGRSGDSGLSKWLEA--KLPAKKAVLGF 248

Query: 245 PFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFL 304
            + GWAW L D+  +   + TDGAA    I  +G I Y+ ++ +I +N G    +D   +
Sbjct: 249 SYCGWAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDN-GAATFHDPAVI 308

Query: 305 IAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAAD 344
             Y Y  TTWI Y+   +I  K+  AKL  +LGYF++++ AD
Sbjct: 309 GFYCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

BLAST of HG10006601 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 235.7 bits (600), Expect = 5.7e-62
Identity = 138/365 (37.81%), Postives = 198/365 (54.25%), Query Frame = 0

Query: 5   QTTKFVNAGYWLASTNDK--------LPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQ 64
           +T   V A YW      +         P + I+  LFTHLF  +A VD+ T++VTI  + 
Sbjct: 9   RTNSIVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAAN 68

Query: 65  DVLINQFSSTVRLRNPDVKTLLSIAGFSER---FAKIATDASNRDVFIKSSIDVARAGKF 124
               + F+ TV+ +N DV+TLLSI G        A +A+++ NR  FI SSID+AR   F
Sbjct: 69  SYQFSSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDF 128

Query: 125 DGLDLQWLYPSSHDDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQY 184
            GLDL W YPS+  +M NF  L+  W  A+VE++   +   LLL A V   P     ++Y
Sbjct: 129 YGLDLAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQYD-GVEY 188

Query: 185 PVDAIKQNLDWVNILSYDFYTPISSSKATSPSSAFNNPKANA-LSANFGIKTWIQSLNNL 244
           PV AI  NLD+VNI++YDFY P  S     P++ F++P   A  S N G++ W+     L
Sbjct: 189 PVKAIADNLDFVNIMAYDFYGPGWSPVTGPPAALFHDPSNPAGRSGNSGLRKWLDEA-KL 248

Query: 245 PHKKIVFGIPFHGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGG 304
           P KK V G P+ GWAW L D+  +   + TDGAA    I  +G I Y+ ++ +I +N G 
Sbjct: 249 PPKKAVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNYIVDN-GA 308

Query: 305 VENNDKRFLIAYIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDDVNTLANA 358
              +D   +  Y Y   TWI Y+   +I  K+  AK   +LGYF++++ AD +   L+ A
Sbjct: 309 ATFHDPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNCG-LSRA 365

BLAST of HG10006601 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-61
Identity = 130/339 (38.35%), Postives = 195/339 (57.52%), Query Frame = 0

Query: 10  VNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSSTVRL 69
           V A YW A +    P A I+ +LFTHLF  +A ++  TY+V +        + F+ TVR 
Sbjct: 26  VKATYWFAESES--PLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVRR 85

Query: 70  RNPDVKTLLSIAG---FSERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSH 129
           RNP VKTLLSI G   ++  FA +A++ ++R +FI SSI +AR+  F GLDL W YPS  
Sbjct: 86  RNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSIT 145

Query: 130 DDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQYPVDAIKQNLDWVN 189
            +M+NF  L+  W  A+  +A +   PRLLL A V    Y  +++ +PV+A+  +LDWVN
Sbjct: 146 TEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADSLDWVN 205

Query: 190 ILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGIPFHGW 249
           +++YDFY   SS    SP+  + +P     S + G++ W Q+   LP KK V G P +G+
Sbjct: 206 LVAYDFYESGSSRVTCSPAPLY-DPITTGPSGDAGVRAWTQA--GLPAKKAVLGFPLYGY 265

Query: 250 AWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFLIAYIY 309
           AW L D+  H+ ++ + G A    I  +G I Y  +++FI +N   +  N    +  Y Y
Sbjct: 266 AWCLTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKATMVYN-SNLVQNYCY 325

Query: 310 YDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDD 346
              TWI Y+   +I  K+  AK   +LGYF+++I ADD+
Sbjct: 326 AKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDN 353

BLAST of HG10006601 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 223.0 bits (567), Expect = 3.8e-58
Identity = 127/342 (37.13%), Postives = 197/342 (57.60%), Query Frame = 0

Query: 10  VNAGYWLASTNDKLPPANINPNLFTHLFVGYAGVDAQTYKVTIDPSQDVLINQFSSTVRL 69
           V A YW  +T+   P  +I+ +LFTHLF  +A ++A++Y++TI        + F+ TV+ 
Sbjct: 7   VKASYWFPATD--FPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTETVQQ 66

Query: 70  RNPDVKTLLSIAGFS---ERFAKIATDASNRDVFIKSSIDVARAGKFDGLDLQWLYPSSH 129
           RNP VKTLLSI G +   + FA +A++  +R  FI+S+I VAR+  F GLDL W YP + 
Sbjct: 67  RNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYPRNE 126

Query: 130 DDMNNFETLVSVWHKAIVEDAINLSLPRLLLVATVSNVPYLQHNIQ---YPVDAIKQNLD 189
           ++M +F  L+  W  A+  ++ +     L+L A V    Y   N Q   YPV AI  +LD
Sbjct: 127 EEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLAISNSLD 186

Query: 190 WVNILSYDFYTPISSSKATSPSSAFNNPKANALSANFGIKTWIQSLNNLPHKKIVFGIPF 249
           W+N+++YDFY P   S  T P ++   P  +  S + G++ W ++   LP KK V G P+
Sbjct: 187 WINLMAYDFYGP-GWSTVTGPPASLYLP-TDGRSGDSGVRDWTEA--GLPAKKAVLGFPY 246

Query: 250 HGWAWKLVDSHRHDVFSKTDGAAKGDNILENGQIYYSDVKKFIEENHGGVENNDKRFLIA 309
           +GWAW L D   +   + T G A    I ++G+I Y  ++ +I +N G  + +D   +  
Sbjct: 247 YGWAWTLADPDVNGYDANTTGPA----ISDDGEISYRQLQTWIVDN-GATKVHDDMMVGD 306

Query: 310 YIYYDTTWIAYESEYTIADKISLAKLNNVLGYFAYNIAADDD 346
           Y Y  TTWI Y+SE +I  K+  AK   +LGYF++++  DD+
Sbjct: 307 YCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6599362.12.2e-14871.91Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1... [more]
KAG7030350.12.8e-14871.63Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022946077.16.3e-14871.63acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mamm... [more]
KAG6599434.12.4e-14771.35Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023521369.13.1e-14771.79acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O818624.1e-6539.94Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A072UR652.2e-4734.57Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
U5N4E32.1e-4532.51Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
A0A072VEP04.9e-4232.58Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
A0A1B1J8Z23.5e-4031.13Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1G2P43.1e-14871.63acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE... [more]
A0A6J1KFT32.2e-14670.79chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1[more]
A0A6J1G4H52.9e-14672.08chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 ... [more]
A0A6J1KB828.3e-14670.51chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1[more]
A0A6J1G4J97.8e-14471.14acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G19810.12.9e-6639.94Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19750.11.3e-6338.89Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19760.15.7e-6237.81Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.11.3e-6138.35Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.13.8e-5837.13Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011583Chitinase IISMARTSM006362g34coord: 9..343
e-value: 4.2E-63
score: 225.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 26..344
e-value: 1.6E-76
score: 259.8
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 5..351
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 5..351
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 243..314
e-value: 1.6E-76
score: 259.8
IPR029070Chitinase insertion domain superfamilySUPERFAMILY54556Chitinase insertion domaincoord: 245..314
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 12..343
e-value: 1.7E-54
score: 185.7
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 9..357
score: 50.149757
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 12..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10006601.1HG10006601.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0008061 chitin binding
molecular_function GO:0016787 hydrolase activity