HG10006583 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10006583
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRING-type domain-containing protein
LocationChr07: 20095906 .. 20097809 (-)
RNA-Seq ExpressionHG10006583
SyntenyHG10006583
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGGAATGTTGCCTGGAGTTGAATGCGCTAGAAGGCGGCGGTTCCACCAGAGCATAACCTCATCGGACTCGCCGTCCACGGCGGCGAAATCAGGATCTACAAGAAGGCCATCCTTCTGTTTATACACAAGCAACCGTGATTTTCATCTCATTTCATCATCTTCTTCACAGGTATTATGAATAAATTCTAAAGAATTCCATCTATGTTCTTCGATTTCTTTTGTTTCTCAGTTTGTTTCCTGGGAAAATCTGATGAATCGTCTTATAATTTTAAAATTAAGCAACGGAGCTCGTCGAATCAAACTTACCAGAACGAGAAGCTTGGTGAAATTGCAAGAGAAGCTAAGGAACGGTTAGATGAGAGGTTGAGAACTCACAGAAAACCTGAAAATCCAAGGTAATTTCTTCGTGAACCACCTTATTTCTTGAGAATTTTATTCATTGTTCTTCAAATCTATTTTTAACTATTCTAAGAAAAAAAACCGTTTGTTACTTGTTAGTAAAATCCAAGAATCAGCAAATTTTAATAGATGATGGGTGCAATTGAAAAGTTTAAAAGATTGAGGATCAAATTGAAAATGCACTAAAAGATTAGGGACTTCATAAATAAATGAAGTTTAAAAATATCATTCTGTTTATGGGTTATGGTTTAGTTCCTATATTTTTTAGATGTTGAATTTTAGTTAACATATATTTAATAGATATTAAATTTAGTCATTGAATTTTTAAGTTAATTTTTTTGAGGAATTTTTTAAAAACAAATTTAATCGAAATTGAATAAATAATTATAATGTCTCATGAAATAAAATACATTTTTGTAGTGAAAAAATTCATAGATAATAATTTTTTTTTAAAAAAAATAAATTGTAAAGGAATTAGTAGTATGAATTAGGTTTAAGATTCATTAAAGTAAAAGACTAAAATTGAATAAATTTCAAATATATGGGATCAAAATAAAAATTGCTTCGAGTTTGTTTTATTTTTTCAGAAAACAACAAAAATTGGTAACACAATAATTAACGTGAAAAAATTCCAAATGAAAAAAAAAACTACAGACGAAAAAAAGATTATACTATGTGAAGAATTATTACAATCCCACAAAAATCTTTTCTCGACCTTAATTATAAGAACAATCTTTCAGAACATGATACTATTCACACAACTAAGCTCGTATACCAATTTGAAATAATTAAAAACCAAATACATATGCCCATTTTTATAGTGTTTGGATTCATCACTTCTTTGAATTTTTTGGCGTGGGTTAACAATTCTTCTAATATTTTGCCAAAAATTCAAAGAATGATTTAAGTAGTAGTTTGGTTTTCATTTTGAGCAAATATTAGGTGAATCGAGGTGCACCTTTAAAAAAATTCAGATAGTTATGTTATTAAAACAAAATAAAATAGTAAAAGATTGATTCATTAGATTATTTTACTAAATGATAAAGAGATGATCAAATTCGAACTCTTAGAACTATAAATACAAAGTCAAAACTACATTAACATAACAAGTTAGTAATTTAAGTTAGCTAAAACCAACATCAAAATTAAAATCATTAGAAATTTCAACCATTTTTTTTTTGGTATAAATGTAGGAGGAAGAGTAGTAGCAATGAAGAAGTAGGGTGGAAGGAATCATCAGAAAAAAATGGTGAGATAAAAAAAGAGAAAACAAAGGGAAAAGAAGAAACAATAACGAAGAAAAGATTCAGTTGGGGGAAGCTAATGAAATGGAAAGCCTCAGAGCAAGAAGAGTGCAGCGTTTGTCTAGAACGGTTCAGAGTGGGCGAGCCACTGGTCCATCTTCAATGCGCCCATAAGTTCCATTCCACCTGCTTGGTTCCTTGGCTCCAAGCCAATGCTCATTGCCCTTGCTGCAGGTTCCCTATTCCTTCTCAATAA

mRNA sequence

ATGGCCGGAATGTTGCCTGGAGTTGAATGCGCTAGAAGGCGGCGGTTCCACCAGAGCATAACCTCATCGGACTCGCCGTCCACGGCGGCGAAATCAGGATCTACAAGAAGGCCATCCTTCTGTTTATACACAAGCAACCGTGATTTTCATCTCATTTCATCATCTTCTTCACAGCAACGGAGCTCGTCGAATCAAACTTACCAGAACGAGAAGCTTGGTGAAATTGCAAGAGAAGCTAAGGAACGGTTAGATGAGAGGTTGAGAACTCACAGAAAACCTGAAAATCCAAGGAGGAAGAGTAGTAGCAATGAAGAAGTAGGGTGGAAGGAATCATCAGAAAAAAATGGTGAGATAAAAAAAGAGAAAACAAAGGGAAAAGAAGAAACAATAACGAAGAAAAGATTCAGTTGGGGGAAGCTAATGAAATGGAAAGCCTCAGAGCAAGAAGAGTGCAGCGTTTGTCTAGAACGGTTCAGAGTGGGCGAGCCACTGGTCCATCTTCAATGCGCCCATAAGTTCCATTCCACCTGCTTGGTTCCTTGGCTCCAAGCCAATGCTCATTGCCCTTGCTGCAGGTTCCCTATTCCTTCTCAATAA

Coding sequence (CDS)

ATGGCCGGAATGTTGCCTGGAGTTGAATGCGCTAGAAGGCGGCGGTTCCACCAGAGCATAACCTCATCGGACTCGCCGTCCACGGCGGCGAAATCAGGATCTACAAGAAGGCCATCCTTCTGTTTATACACAAGCAACCGTGATTTTCATCTCATTTCATCATCTTCTTCACAGCAACGGAGCTCGTCGAATCAAACTTACCAGAACGAGAAGCTTGGTGAAATTGCAAGAGAAGCTAAGGAACGGTTAGATGAGAGGTTGAGAACTCACAGAAAACCTGAAAATCCAAGGAGGAAGAGTAGTAGCAATGAAGAAGTAGGGTGGAAGGAATCATCAGAAAAAAATGGTGAGATAAAAAAAGAGAAAACAAAGGGAAAAGAAGAAACAATAACGAAGAAAAGATTCAGTTGGGGGAAGCTAATGAAATGGAAAGCCTCAGAGCAAGAAGAGTGCAGCGTTTGTCTAGAACGGTTCAGAGTGGGCGAGCCACTGGTCCATCTTCAATGCGCCCATAAGTTCCATTCCACCTGCTTGGTTCCTTGGCTCCAAGCCAATGCTCATTGCCCTTGCTGCAGGTTCCCTATTCCTTCTCAATAA

Protein sequence

MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQRSSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIKKEKTKGKEETITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLVPWLQANAHCPCCRFPIPSQ
Homology
BLAST of HG10006583 vs. NCBI nr
Match: XP_038891102.1 (probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida])

HSP 1 Score: 342.8 bits (878), Expect = 2.0e-90
Identity = 178/199 (89.45%), Postives = 186/199 (93.47%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60
           MAGMLPGVECARRRRFHQSI SSDSPSTAAKSGSTRRPSFCLYTSNRDFH  SSSSSQ R
Sbjct: 1   MAGMLPGVECARRRRFHQSIASSDSPSTAAKSGSTRRPSFCLYTSNRDFHSTSSSSSQHR 60

Query: 61  SSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIKK 120
           SSSNQ YQNEKLGEIAREAKERLDERLRTHRKPEN RRK S NE+VGWK SSEKNGE +K
Sbjct: 61  SSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENSRRK-SENEKVGWKGSSEKNGE-EK 120

Query: 121 EKTKGKEET--ITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCL 180
           E+TKGKEE+  ITKKRF+WGKLMKW+ASEQEEC+VCLERFRVGEPLVHL CAHKFHSTCL
Sbjct: 121 ERTKGKEESIIITKKRFNWGKLMKWRASEQEECTVCLERFRVGEPLVHLPCAHKFHSTCL 180

Query: 181 VPWLQANAHCPCCRFPIPS 198
           VPWLQAN+HCPCCRFPIPS
Sbjct: 181 VPWLQANSHCPCCRFPIPS 197

BLAST of HG10006583 vs. NCBI nr
Match: XP_004139268.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucumis sativus] >KGN60748.1 hypothetical protein Csa_019469 [Cucumis sativus])

HSP 1 Score: 317.4 bits (812), Expect = 8.8e-83
Identity = 173/203 (85.22%), Postives = 180/203 (88.67%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60
           MAGMLPGVECARRRRFHQSI SSDSP TA KSGSTRRPSFCLYTSNRDFHLISSSSSQQR
Sbjct: 1   MAGMLPGVECARRRRFHQSINSSDSPFTAGKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIKK 120
           S +NQ YQN+KLGEIAREAKERLDERLRTHRKPEN R+K ++NEEV  KES EKN   KK
Sbjct: 61  SLTNQAYQNDKLGEIAREAKERLDERLRTHRKPENSRKK-NNNEEVKGKESREKNNGEKK 120

Query: 121 E--KTKGKEETITKKRFSWG-KLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTC 180
           E   TKGKEE  TKKRFSWG KLMKWKASEQEECSVCLERFR+ EPL+HL CAHKFHSTC
Sbjct: 121 EMKNTKGKEEE-TKKRFSWGRKLMKWKASEQEECSVCLERFRLSEPLLHLPCAHKFHSTC 180

Query: 181 LVPWLQANAHCPCCRFPI--PSQ 199
           LVPWLQANAHCPCCRFPI  PSQ
Sbjct: 181 LVPWLQANAHCPCCRFPIAVPSQ 201

BLAST of HG10006583 vs. NCBI nr
Match: XP_008456709.1 (PREDICTED: RING-H2 finger protein ATL16 [Cucumis melo])

HSP 1 Score: 316.2 bits (809), Expect = 2.0e-82
Identity = 167/195 (85.64%), Postives = 175/195 (89.74%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60
           MAGMLPGVECARRRRFH SI SSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR
Sbjct: 1   MAGMLPGVECARRRRFHHSINSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIKK 120
           S +N+ +QNEKLGEIAREAKERLDERLRTHRKPEN R K+S NEEV WKES EKN   +K
Sbjct: 61  SLTNKAHQNEKLGEIAREAKERLDERLRTHRKPENSRSKNSKNEEVKWKESREKNNGERK 120

Query: 121 E--KTKGKEETITKKRFSWG-KLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTC 180
           E   TKGKEE  T+KRFSWG KLMKWKASEQ+ECSVCLERFR GEPL+HL CAHKFHSTC
Sbjct: 121 EMRSTKGKEEE-TRKRFSWGRKLMKWKASEQDECSVCLERFRSGEPLLHLPCAHKFHSTC 180

Query: 181 LVPWLQANAHCPCCR 193
           LVPWLQANAHCPCCR
Sbjct: 181 LVPWLQANAHCPCCR 194

BLAST of HG10006583 vs. NCBI nr
Match: KAG7030344.1 (putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 314.7 bits (805), Expect = 5.7e-82
Identity = 165/198 (83.33%), Postives = 172/198 (86.87%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSS-SQQ 60
           MAGMLPGVECARRRRFHQSIT SD+PSTAAK GSTRRPSFCLYTSNRDFH+ SSSS SQQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIK 120
           RSSS+Q YQNEKLGEIAREAKERLDERLRTHRKPENPR+KS      G K S E     K
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGE----GLKNSLE-----K 120

Query: 121 KEKTKGKEETITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLV 180
           KEK KGKEE+ITKKRF+WGKLMKWKASEQEEC+VCLE FR GEPLVHL CAHKFHS CLV
Sbjct: 121 KEKAKGKEESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLV 180

Query: 181 PWLQANAHCPCCRFPIPS 198
           PWLQ NAHCPCCRFP PS
Sbjct: 181 PWLQTNAHCPCCRFPFPS 189

BLAST of HG10006583 vs. NCBI nr
Match: XP_023538036.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 311.2 bits (796), Expect = 6.3e-81
Identity = 165/198 (83.33%), Postives = 172/198 (86.87%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60
           MAGMLPGVECARRRRFHQSITSSDS STAAK GSTRR SFCLYTSNRDFHL +SSSSQQR
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYTSNRDFHL-TSSSSQQR 60

Query: 61  SSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIKK 120
            SSN  YQNEKLG+ AREAKERLDERLRTHRKPEN R+KSSS EEVG          +KK
Sbjct: 61  CSSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRKKSSS-EEVG----------LKK 120

Query: 121 EKTKGKEETITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLVP 180
           EK KGKEET TKKRFSWGKLMKWKASEQEEC+VCLERFR  EPLVHL CAHKFHSTCLVP
Sbjct: 121 EKAKGKEETTTKKRFSWGKLMKWKASEQEECTVCLERFRAVEPLVHLPCAHKFHSTCLVP 180

Query: 181 WLQANAHCPCCRFPIPSQ 199
           WLQANAHCPCCR+PI +Q
Sbjct: 181 WLQANAHCPCCRYPILAQ 186

BLAST of HG10006583 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.5e-11
Identity = 26/55 (47.27%), Postives = 36/55 (65.45%), Query Frame = 0

Query: 144 KASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLVPWLQANAHCPCCRFPIPSQ 199
           K  E   CSVCL+   VG     + C HKFHS+C++PWL+ ++ CP CRF +PS+
Sbjct: 192 KIEEVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPSE 246

BLAST of HG10006583 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.0e-09
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = 0

Query: 146 SEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLVPWLQANAHCPCCRFPIPS 198
           SE  +C+VC++ F  G  +  + C H FH  CL+PWL+ +  CP CRF +P+
Sbjct: 211 SEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPT 262

BLAST of HG10006583 vs. ExPASy Swiss-Prot
Match: Q9C1X4 (Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP32A8.03c PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 25/69 (36.23%), Postives = 39/69 (56.52%), Query Frame = 0

Query: 127 EETITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLVPWLQANA 186
           E+ I K +    K  K    E+ EC++C+E F++ + ++ L C H FH  C+ PWL+ N 
Sbjct: 374 EDVIAKMKVQ--KPPKELIDEEGECTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNG 433

Query: 187 HCPCCRFPI 196
            C  CR P+
Sbjct: 434 TCAICRAPV 440

BLAST of HG10006583 vs. ExPASy Swiss-Prot
Match: Q08D68 (E3 ubiquitin-protein ligase ZNRF3 OS=Xenopus tropicalis OX=8364 GN=znrf3 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 7.4e-08
Identity = 32/82 (39.02%), Postives = 43/82 (52.44%), Query Frame = 0

Query: 117 EIKKEKTKGKEETITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHST 176
           E +K K KGK      +  S G L    +S   +C++CLE++  GE L  + C H+FH  
Sbjct: 236 ETRKFKAKGK----VPREGSCGGLDTLSSSSTSDCAICLEKYIDGEELRVIPCTHRFHKR 295

Query: 177 CLVPWLQANAHCPCCRFPIPSQ 199
           C+ PWL  N  CP CR  I  Q
Sbjct: 296 CVDPWLLQNHTCPHCRHNIIEQ 313

BLAST of HG10006583 vs. ExPASy Swiss-Prot
Match: Q4KLR8 (E3 ubiquitin-protein ligase ZNRF3 OS=Xenopus laevis OX=8355 GN=znrf3 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.7e-08
Identity = 32/82 (39.02%), Postives = 43/82 (52.44%), Query Frame = 0

Query: 117 EIKKEKTKGKEETITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHST 176
           E +K K KGK      +  S G L    +S   +C++CLE++  GE L  + C H+FH  
Sbjct: 172 ETRKFKAKGK----VSREGSCGGLDTLSSSSISDCAICLEKYIDGEELRVIPCTHRFHKR 231

Query: 177 CLVPWLQANAHCPCCRFPIPSQ 199
           C+ PWL  N  CP CR  I  Q
Sbjct: 232 CVDPWLLQNHTCPHCRHNIIEQ 249

BLAST of HG10006583 vs. ExPASy TrEMBL
Match: A0A0A0LG47 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 4.3e-83
Identity = 173/203 (85.22%), Postives = 180/203 (88.67%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60
           MAGMLPGVECARRRRFHQSI SSDSP TA KSGSTRRPSFCLYTSNRDFHLISSSSSQQR
Sbjct: 1   MAGMLPGVECARRRRFHQSINSSDSPFTAGKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIKK 120
           S +NQ YQN+KLGEIAREAKERLDERLRTHRKPEN R+K ++NEEV  KES EKN   KK
Sbjct: 61  SLTNQAYQNDKLGEIAREAKERLDERLRTHRKPENSRKK-NNNEEVKGKESREKNNGEKK 120

Query: 121 E--KTKGKEETITKKRFSWG-KLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTC 180
           E   TKGKEE  TKKRFSWG KLMKWKASEQEECSVCLERFR+ EPL+HL CAHKFHSTC
Sbjct: 121 EMKNTKGKEEE-TKKRFSWGRKLMKWKASEQEECSVCLERFRLSEPLLHLPCAHKFHSTC 180

Query: 181 LVPWLQANAHCPCCRFPI--PSQ 199
           LVPWLQANAHCPCCRFPI  PSQ
Sbjct: 181 LVPWLQANAHCPCCRFPIAVPSQ 201

BLAST of HG10006583 vs. ExPASy TrEMBL
Match: A0A1S3C563 (RING-H2 finger protein ATL16 OS=Cucumis melo OX=3656 GN=LOC103496570 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 9.5e-83
Identity = 167/195 (85.64%), Postives = 175/195 (89.74%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60
           MAGMLPGVECARRRRFH SI SSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR
Sbjct: 1   MAGMLPGVECARRRRFHHSINSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIKK 120
           S +N+ +QNEKLGEIAREAKERLDERLRTHRKPEN R K+S NEEV WKES EKN   +K
Sbjct: 61  SLTNKAHQNEKLGEIAREAKERLDERLRTHRKPENSRSKNSKNEEVKWKESREKNNGERK 120

Query: 121 E--KTKGKEETITKKRFSWG-KLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTC 180
           E   TKGKEE  T+KRFSWG KLMKWKASEQ+ECSVCLERFR GEPL+HL CAHKFHSTC
Sbjct: 121 EMRSTKGKEEE-TRKRFSWGRKLMKWKASEQDECSVCLERFRSGEPLLHLPCAHKFHSTC 180

Query: 181 LVPWLQANAHCPCCR 193
           LVPWLQANAHCPCCR
Sbjct: 181 LVPWLQANAHCPCCR 194

BLAST of HG10006583 vs. ExPASy TrEMBL
Match: A0A6J1G4D6 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC111450678 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.7e-79
Identity = 158/198 (79.80%), Postives = 165/198 (83.33%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSS-SQQ 60
           MAGMLPGVECARRRRFHQSITSSD+PSTAAK GSTRRPSFCLYTSNRDFH+ SSSS SQQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIK 120
           RSSS+Q YQNEKLGEIAREAKERLDERLRTHRKPENPR+KS      GW           
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGE----GW----------- 120

Query: 121 KEKTKGKEETITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLV 180
                 KEE+ITKKRF+WGKLMKWKASEQEEC+VCLE FR GEPLVHL CAHKFHS CLV
Sbjct: 121 ------KEESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLV 177

Query: 181 PWLQANAHCPCCRFPIPS 198
           PWLQ NAHCPCCRFP PS
Sbjct: 181 PWLQTNAHCPCCRFPFPS 177

BLAST of HG10006583 vs. ExPASy TrEMBL
Match: A0A6J1HQI5 (probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465688 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.1e-78
Identity = 161/198 (81.31%), Postives = 168/198 (84.85%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60
           MAGMLPGVECARRRRFHQSITSS+S STAAK GSTRR SFCLYTSNRDFHL +SSSSQQR
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHL-TSSSSQQR 60

Query: 61  SSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIKK 120
            SSN  YQNEKLG+ AREAKERLDERLRTHRKPEN R KSSS EEV           +KK
Sbjct: 61  CSSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSS-EEV----------RLKK 120

Query: 121 EKTKGKEETITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLVP 180
           E  KGKEET TKKRFSWGKLMKWKASEQEEC+VCLERFR  EPLVHL CAHKFHS CLVP
Sbjct: 121 ENVKGKEETTTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVP 180

Query: 181 WLQANAHCPCCRFPIPSQ 199
           WLQANAHCPCCR+PI +Q
Sbjct: 181 WLQANAHCPCCRYPILAQ 186

BLAST of HG10006583 vs. ExPASy TrEMBL
Match: A0A6J1FHS8 (E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444265 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 4.1e-78
Identity = 159/198 (80.30%), Postives = 169/198 (85.35%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60
           MAGMLPGVECARRRRFHQSITSSDS STAAK GSTRR SFCLY SNRDFHL +SSSSQQR
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYASNRDFHL-TSSSSQQR 60

Query: 61  SSSNQTYQNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESSEKNGEIKK 120
            SSN  YQ+EKLG+ AREAKERLDERLRTHRKPEN R+KSSS EEVG          +KK
Sbjct: 61  CSSNHAYQDEKLGQAAREAKERLDERLRTHRKPENSRKKSSS-EEVG----------LKK 120

Query: 121 EKTKGKEETITKKRFSWGKLMKWKASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLVP 180
           EK KGKEET TKKRF WGKLMKWKASEQEEC+VCLE+FR  EPLVHL CAHKFHS CLVP
Sbjct: 121 EKAKGKEETTTKKRFGWGKLMKWKASEQEECTVCLEKFRATEPLVHLPCAHKFHSACLVP 180

Query: 181 WLQANAHCPCCRFPIPSQ 199
           WLQANA+CPCCR+PI +Q
Sbjct: 181 WLQANAYCPCCRYPILAQ 186

BLAST of HG10006583 vs. TAIR 10
Match: AT2G15580.1 (RING/U-box superfamily protein )

HSP 1 Score: 142.9 bits (359), Expect = 2.8e-34
Identity = 97/206 (47.09%), Postives = 127/206 (61.65%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKSGS--TRRPSFCLYTSNRDFHLISSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA +G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  SSQQRSSSNQTY---QNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKESS 120
            S +RS  N++Y    +EKL   A+EAK+RL++RLR       P R   + ++ G K   
Sbjct: 61  FS-ERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRI------PPRTRQNGKDKGNKLEQ 120

Query: 121 EKNGEIKKEKTKGKEETITKKRFSWGKLMKW---KASEQEECSVCLERFRVGEPLVHLQC 180
            K   +    T    E +  K+ S G+LM+W   +  EQ++C++CL+RF+ GE LVHL C
Sbjct: 121 GKGKPLGDLPT----EVVGLKK-SRGRLMEWFKRRVREQQDCAICLDRFKKGETLVHLPC 180

Query: 181 AHKFHSTCLVPWLQANAHCPCCRFPI 196
           AHKFHS CL+PWL  N +CP CR  I
Sbjct: 181 AHKFHSICLLPWLDTNVYCPYCRTDI 194

BLAST of HG10006583 vs. TAIR 10
Match: AT2G15580.2 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-13
Identity = 56/118 (47.46%), Postives = 72/118 (61.02%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKSGS--TRRPSFCLYTSNRDFHLISSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA +G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  SSQQRSSSNQTY---QNEKLGEIAREAKERLDERLRTHRKPENPRRKSSSNEEVGWKE 111
            S +RS  N++Y    +EKL   A+EAK+RL++RLR    P     K    + + W +
Sbjct: 61  FS-ERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRI--PPRTSSGKMVKTKGINWSK 115

BLAST of HG10006583 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.5e-11
Identity = 25/54 (46.30%), Postives = 36/54 (66.67%), Query Frame = 0

Query: 144 KASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLVPWLQANAHCPCCRFPIPS 198
           K  E  +CSVCL+ F +G     + C HKFHS CL+PWL+ ++ CP CR+ +P+
Sbjct: 216 KIEETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPA 269

BLAST of HG10006583 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.3e-11
Identity = 26/54 (48.15%), Postives = 34/54 (62.96%), Query Frame = 0

Query: 144 KASEQEECSVCLERFRVGEPLVHLQCAHKFHSTCLVPWLQANAHCPCCRFPIPS 198
           K  E  +CSVCL+ F  G     + C HKFH  C+VPWL+ ++ CP CRF +PS
Sbjct: 252 KIMEPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLELHSSCPVCRFELPS 305

BLAST of HG10006583 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 5.6e-11
Identity = 23/48 (47.92%), Postives = 34/48 (70.83%), Query Frame = 0

Query: 150 ECSVCLERFRVGEPLVHLQCAHKFHSTCLVPWLQANAHCPCCRFPIPS 198
           +CSVCL+ F +G     + C HKFHS CL+PWL+ ++ CP CR+ +P+
Sbjct: 224 QCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPT 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891102.12.0e-9089.45probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida][more]
XP_004139268.18.8e-8385.22probable E3 ubiquitin-protein ligase RHY1A [Cucumis sativus] >KGN60748.1 hypothe... [more]
XP_008456709.12.0e-8285.64PREDICTED: RING-H2 finger protein ATL16 [Cucumis melo][more]
KAG7030344.15.7e-8283.33putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subs... [more]
XP_023538036.16.3e-8183.33probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q6AVN25.5e-1147.27E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
Q8LPN73.0e-0942.31E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q9C1X41.1e-0836.23Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (stra... [more]
Q08D687.4e-0839.02E3 ubiquitin-protein ligase ZNRF3 OS=Xenopus tropicalis OX=8364 GN=znrf3 PE=1 SV... [more]
Q4KLR89.7e-0839.02E3 ubiquitin-protein ligase ZNRF3 OS=Xenopus laevis OX=8355 GN=znrf3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LG474.3e-8385.22RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 P... [more]
A0A1S3C5639.5e-8385.64RING-H2 finger protein ATL16 OS=Cucumis melo OX=3656 GN=LOC103496570 PE=4 SV=1[more]
A0A6J1G4D61.7e-7979.80probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HQI51.1e-7881.31probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1FHS84.1e-7880.30E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT2G15580.12.8e-3447.09RING/U-box superfamily protein [more]
AT2G15580.22.1e-1347.46RING/U-box superfamily protein [more]
AT1G55530.11.5e-1146.30RING/U-box superfamily protein [more]
AT5G56340.13.3e-1148.15RING/U-box superfamily protein [more]
AT3G13430.15.6e-1147.92RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 151..191
e-value: 2.6E-4
score: 30.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 150..192
e-value: 1.2E-10
score: 41.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 151..192
score: 12.090947
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 121..197
e-value: 3.6E-17
score: 63.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..39
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..127
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..39
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..127
NoneNo IPR availablePANTHERPTHR22765:SF204RING/U-BOX SUPERFAMILY PROTEINcoord: 1..192
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 1..192
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 150..192
e-value: 1.64373E-15
score: 65.4009
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 138..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10006583.1HG10006583.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
molecular_function GO:0061630 ubiquitin protein ligase activity