HG10006553 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10006553
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr07: 19802938 .. 19803570 (-)
RNA-Seq Expression	HG10006553
Synteny	HG10006553

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGTGTCGAAAGGGAAATACACCAAGTTACAGGGCAAGCAATGGTCCACATTCCACCTTTCCAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTCTCGCTCTCCGATTCTTCTCTCCTCCGGAAACTTCTCATCGGAATCTACCCCATCGTCTCGCTTCCCTCCGGCATGCAGCCCTTGAAAGGTACTCCGGCAACGCAATGGCTGTGATTCCTTAAGCTTGTTGCATGAATCAAGTGAATTTGATTTTGGTAATTTTGTTGTTGTTGTAGTGATGGGTTGGGGAAAAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTTTATCACAATTTCTTGGTAAGTTTTCTAGAGTTATGGTTTCTTTTTGGCTTCATGTTTCATTTTGTCATTTTTTGAAAAAAAAAAGTGTTTGGTTAAAATGTTGGCTGATAGAATGGTGGGGACAGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCTCACATGGAGAAATCAACTGTGGTTGATAGCAAAACTGGCAAGAGTGTGGATAGCAGTTCTGTATTTGTCATTTTCATTGTTGTCGTCACTACAACACTTGTCGATTATTGA

mRNA sequence

ATGGCGGTGTCGAAAGGGAAATACACCAAGTTACAGGGCAAGCAATGGTCCACATTCCACCTTTCCAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTCTCGCTCTCCGATTCTTCTCTCCTCCGGAAACTTCTCATCGGAATCTACCCCATCGTCTCGCTTCCCTCCGGCATGCAGCCCTTGAAAGTGATGGGTTGGGGAAAAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTTTATCACAATTTCTTGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCTCACATGGAGAAATCAACTGTGGTTGATAGCAAAACTGGCAAGAGTGTGGATAGCAGTTCTGTATTTGTCATTTTCATTGTTGTCGTCACTACAACACTTGTCGATTATTGA

Coding sequence (CDS)

ATGGCGGTGTCGAAAGGGAAATACACCAAGTTACAGGGCAAGCAATGGTCCACATTCCACCTTTCCAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTCTCGCTCTCCGATTCTTCTCTCCTCCGGAAACTTCTCATCGGAATCTACCCCATCGTCTCGCTTCCCTCCGGCATGCAGCCCTTGAAAGTGATGGGTTGGGGAAAAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTTTATCACAATTTCTTGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCTCACATGGAGAAATCAACTGTGGTTGATAGCAAAACTGGCAAGAGTGTGGATAGCAGTTCTGTATTTGTCATTTTCATTGTTGTCGTCACTACAACACTTGTCGATTATTGA

Protein sequence

MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASLRHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSVDSSSVFVIFIVVVTTTLVDY

Homology

[Close]

BLAST of HG10006553 vs. NCBI nr
Match: XP_038889689.1 (probable prolyl 4-hydroxylase 3 [Benincasa hispida])

HSP 1 Score: 245.0 bits (624), Expect = 4.0e-61
Identity = 118/126 (93.65%), Postives = 124/126 (98.41%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MA+SKGKYTK+QGK+WSTF LSKMIMALVLALGFFMLLALRFFSPPETSHRNLPH LAS+
Sbjct: 1   MALSKGKYTKIQGKKWSTFELSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHHLASV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           RH+A+ESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT
Sbjct: 61  RHSAVESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GKSVDS 127
           GKSVDS
Sbjct: 121 GKSVDS 126

[Close]

BLAST of HG10006553 vs. NCBI nr
Match: XP_008456388.1 (PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo])

HSP 1 Score: 224.6 bits (571), Expect = 5.6e-55
Identity = 112/126 (88.89%), Postives = 116/126 (92.06%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MAVSKGKY KLQGK+WSTF LSKMIMALVLALGFFML+ALRFFSPPETSH    HRL S+
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSH----HRLPSV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           R  A +SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GKSVDS 127
           GKSVDS
Sbjct: 121 GKSVDS 122

[Close]

BLAST of HG10006553 vs. NCBI nr
Match: KAA0063574.1 (putative prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo var. makuwa] >TYJ97902.1 putative prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 224.6 bits (571), Expect = 5.6e-55
Identity = 112/126 (88.89%), Postives = 116/126 (92.06%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MAVSKGKY KLQGK+WSTF LSKMIMALVLALGFFML+ALRFFSPPETSH    HRL S+
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSH----HRLPSV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           R  A +SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GKSVDS 127
           GKSVDS
Sbjct: 121 GKSVDS 122

[Close]

BLAST of HG10006553 vs. NCBI nr
Match: XP_008456383.1 (PREDICTED: probable prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo])

HSP 1 Score: 224.6 bits (571), Expect = 5.6e-55
Identity = 112/126 (88.89%), Postives = 116/126 (92.06%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MAVSKGKY KLQGK+WSTF LSKMIMALVLALGFFML+ALRFFSPPETSH    HRL S+
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSH----HRLPSV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           R  A +SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GKSVDS 127
           GKSVDS
Sbjct: 121 GKSVDS 122

[Close]

BLAST of HG10006553 vs. NCBI nr
Match: XP_011648735.2 (probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical protein Csa_019368 [Cucumis sativus])

HSP 1 Score: 221.9 bits (564), Expect = 3.7e-54
Identity = 109/126 (86.51%), Postives = 116/126 (92.06%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MA+SKGKY KLQG++WSTF LSKMIMALVLALGFFML+ALRFFSPPETSH    HR +S+
Sbjct: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSH----HRFSSV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           RH A  SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT
Sbjct: 61  RHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GKSVDS 127
           G+SVDS
Sbjct: 121 GESVDS 122

[Close]

BLAST of HG10006553 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 9.1e-32
Identity = 73/125 (58.40%), Postives = 90/125 (72.00%), Query Frame = 0


Query: 3   VSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASLRH 62
           ++K ++++ Q ++WST  L  + M  +L +   MLLA   FS P  +  + P  L+  R 
Sbjct: 1   MAKLRHSRFQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRR 60

Query: 63  AALE-SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG 122
           AA E S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVDS+TG
Sbjct: 61  AATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETG 120

Query: 123 KSVDS 127
           KS DS
Sbjct: 121 KSKDS 124

[Close]

BLAST of HG10006553 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 9.4e-21
Identity = 54/109 (49.54%), Postives = 70/109 (64.22%), Query Frame = 0


Query: 22  SKMIMALVLALGFFMLLALRF---FSPPETSHRNLPHRLASLRHAALESDGL-GKRGDQW 81
           S ++ A+++   F +L+ L F     P   +  +  + L S+    L+  G    + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSVDS 127
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD KTGKS DS
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDS 126

[Close]

BLAST of HG10006553 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 3.6e-20
Identity = 55/125 (44.00%), Postives = 75/125 (60.00%), Query Frame = 0


Query: 7   KYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSH-RNLPHRLASLRHAAL 66
           K  +L+ K   +F      + +++     +L+ L  FS P T+   ++P  L ++     
Sbjct: 4   KPKQLRNKPRKSFSTQTFTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQTIQ 63

Query: 67  ESDGLGK----RGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG 126
           E +  G      GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD KTG
Sbjct: 64  ERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTG 123

[Close]

BLAST of HG10006553 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.8e-16
Identity = 54/122 (44.26%), Postives = 70/122 (57.38%), Query Frame = 0


Query: 7   KYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLAS--LRHAA 66
           +Y   +    ST   + +I+ LV+ L   +LL L   S P  +  +      +  +R + 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVIL---ILLGLGILSLPNANRNSSKTNDLTNIVRKSE 69

Query: 67  LESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 126
             S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD KTG S 
Sbjct: 70  TSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSK 128

[Close]

BLAST of HG10006553 vs. ExPASy Swiss-Prot
Match: Q9ZW86 (Prolyl 4-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=P4H1 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.9e-09
Identity = 28/48 (58.33%), Postives = 35/48 (72.92%), Query Frame = 0


Query: 79  EFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSVDS 127
           E +SW PR  V H+FLS EEC YL ++A+P ++ STVVD KTGK V S
Sbjct: 78  EVVSWSPRIIVLHDFLSPEECEYLKAIARPRLQVSTVVDVKTGKGVKS 125

[Close]

BLAST of HG10006553 vs. ExPASy TrEMBL
Match: A0A5D3BDQ0 (Putative prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001180 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.7e-55
Identity = 112/126 (88.89%), Postives = 116/126 (92.06%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MAVSKGKY KLQGK+WSTF LSKMIMALVLALGFFML+ALRFFSPPETSH    HRL S+
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSH----HRLPSV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           R  A +SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GKSVDS 127
           GKSVDS
Sbjct: 121 GKSVDS 122

[Close]

BLAST of HG10006553 vs. ExPASy TrEMBL
Match: A0A1S3C2P6 (probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496331 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.7e-55
Identity = 112/126 (88.89%), Postives = 116/126 (92.06%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MAVSKGKY KLQGK+WSTF LSKMIMALVLALGFFML+ALRFFSPPETSH    HRL S+
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSH----HRLPSV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           R  A +SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GKSVDS 127
           GKSVDS
Sbjct: 121 GKSVDS 122

[Close]

BLAST of HG10006553 vs. ExPASy TrEMBL
Match: A0A1S3C367 (probable prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496331 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.7e-55
Identity = 112/126 (88.89%), Postives = 116/126 (92.06%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MAVSKGKY KLQGK+WSTF LSKMIMALVLALGFFML+ALRFFSPPETSH    HRL S+
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSH----HRLPSV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           R  A +SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT
Sbjct: 61  RRTAFQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GKSVDS 127
           GKSVDS
Sbjct: 121 GKSVDS 122

[Close]

BLAST of HG10006553 vs. ExPASy TrEMBL
Match: A0A5D3BGX5 (Putative prolyl 4-hydroxylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001170 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.0e-53
Identity = 111/127 (87.40%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MAVS GKY KLQGK+WSTF LSKMIMALVLALGFFML AL FFSPPETSH    HRL+S+
Sbjct: 1   MAVSIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSH----HRLSSV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           RH A  SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+T
Sbjct: 61  RHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSET 120

Query: 121 GKSVDSS 128
           GKSVDSS
Sbjct: 121 GKSVDSS 123

[Close]

BLAST of HG10006553 vs. ExPASy TrEMBL
Match: A0A1S4DZG7 (probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103496316 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.0e-53
Identity = 111/127 (87.40%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MAVSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASL 60
           MAVS GKY KLQGK+WSTF LSKMIMALVLALGFFML AL FFSPPETSH    HRL+S+
Sbjct: 1   MAVSIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSH----HRLSSV 60

Query: 61  RHAALESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           RH A  SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+T
Sbjct: 61  RHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSET 120

Query: 121 GKSVDSS 128
           GKSVDSS
Sbjct: 121 GKSVDSS 123

[Close]

BLAST of HG10006553 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 137.9 bits (346), Expect = 6.5e-33
Identity = 73/125 (58.40%), Postives = 90/125 (72.00%), Query Frame = 0


Query: 3   VSKGKYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLASLRH 62
           ++K ++++ Q ++WST  L  + M  +L +   MLLA   FS P  +  + P  L+  R 
Sbjct: 1   MAKLRHSRFQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRR 60

Query: 63  AALE-SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG 122
           AA E S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVDS+TG
Sbjct: 61  AATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETG 120

Query: 123 KSVDS 127
           KS DS
Sbjct: 121 KSKDS 124

[Close]

BLAST of HG10006553 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 6.7e-22
Identity = 54/109 (49.54%), Postives = 70/109 (64.22%), Query Frame = 0


Query: 22  SKMIMALVLALGFFMLLALRF---FSPPETSHRNLPHRLASLRHAALESDGL-GKRGDQW 81
           S ++ A+++   F +L+ L F     P   +  +  + L S+    L+  G    + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSVDS 127
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD KTGKS DS
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDS 126

[Close]

BLAST of HG10006553 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 2.6e-21
Identity = 55/125 (44.00%), Postives = 75/125 (60.00%), Query Frame = 0


Query: 7   KYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSH-RNLPHRLASLRHAAL 66
           K  +L+ K   +F      + +++     +L+ L  FS P T+   ++P  L ++     
Sbjct: 4   KPKQLRNKPRKSFSTQTFTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQTIQ 63

Query: 67  ESDGLGK----RGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG 126
           E +  G      GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD KTG
Sbjct: 64  ERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKTG 123

[Close]

BLAST of HG10006553 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.4e-19
Identity = 53/109 (48.62%), Postives = 68/109 (62.39%), Query Frame = 0


Query: 22  SKMIMALVLALGFFMLLALRF---FSPPETSHRNLPHRLASLRHAALESDGL-GKRGDQW 81
           S ++ A+++   F +L+ L F     P   +  +  + L S+    L+  G    + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSVDS 127
           VE ISWEPRA VYHNFL  EEC YLI LAKPHMEKSTVVD KTGKS DS
Sbjct: 78  VEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDEKTGKSTDS 124

[Close]

BLAST of HG10006553 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.3e-17
Identity = 54/122 (44.26%), Postives = 70/122 (57.38%), Query Frame = 0


Query: 7   KYTKLQGKQWSTFHLSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHRLAS--LRHAA 66
           +Y   +    ST   + +I+ LV+ L   +LL L   S P  +  +      +  +R + 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVIL---ILLGLGILSLPNANRNSSKTNDLTNIVRKSE 69

Query: 67  LESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 126
             S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD KTG S 
Sbjct: 70  TSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSK 128

The following BLAST results are available for this feature:

BLAST of HG10006553 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889689.1	4.0e-61	93.65	probable prolyl 4-hydroxylase 3 [Benincasa hispida]	[more]
XP_008456388.1	5.6e-55	88.89	PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo]	[more]
KAA0063574.1	5.6e-55	88.89	putative prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo var. makuwa] >TYJ97902....	[more]
XP_008456383.1	5.6e-55	88.89	PREDICTED: probable prolyl 4-hydroxylase 3 isoform X1 [Cucumis melo]	[more]
XP_011648735.2	3.7e-54	86.51	probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical pro...	[more]

BLAST of HG10006553 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LN20	9.1e-32	58.40	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]
F4JZ24	9.4e-21	49.54	Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S...	[more]
F4JNU8	3.6e-20	44.00	Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=...	[more]
Q24JN5	1.8e-16	44.26	Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1	[more]
Q9ZW86	4.9e-09	58.33	Prolyl 4-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=P4H1 PE=1 SV=1	[more]

BLAST of HG10006553 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BDQ0	2.7e-55	88.89	Putative prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3C2P6	2.7e-55	88.89	probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034963...	[more]
A0A1S3C367	2.7e-55	88.89	probable prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034963...	[more]
A0A5D3BGX5	2.0e-53	87.40	Putative prolyl 4-hydroxylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S4DZG7	2.0e-53	87.40	probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103496316 PE=4 SV=...	[more]

BLAST of HG10006553 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20270.1	6.5e-33	58.40	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.1	6.7e-22	49.54	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G35810.1	2.6e-21	44.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.2	1.4e-19	48.62	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17720.1	1.3e-17	44.26	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 78..136 e-value: 2.1E-12 score: 49.2
None	No IPR available	PANTHER	PTHR10869:SF169	PROLYL 4-HYDROXYLASE 3-RELATED	coord: 4..127
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 4..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10006553.1	HG10006553.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen