HG10006386 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10006386
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGDSL esterase/lipase
LocationChr07: 17975213 .. 17978948 (-)
RNA-Seq ExpressionHG10006386
SyntenyHG10006386
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACGAACTAATAATTACCTCATTGCAGCTCTATCATTCCACATAATTTGGCTGCTATTTCTTAGCAAACCAGGAAGTGCTTTAAAGCCTAAAAGTCCTCGGTCATTCCTTGCCATTCTCATATTTGGTGATTCTACCGTAGATACCGGAAATAACAACTTTATTTCCATCCTTTTCAAAGCCAATTACTTTCCATACGGCCAAGATTTTCCTGGCCATGTTAGTAGTGGAAGGTTTAGTGATGGAAAAATCATTCCTGATATGGTGGCTTCTAGGTTAGGGATAAAGGAGTTTGTTCCTCCATTTTTGGATCCGAAATTGTCGAATGATGATGTGAAAACAGGGGTTAGTTTTGCCTCGGCTGGCACTGGCTTCGATGATCTGACAGCCGCTATATCTAGAGTCATTGCTGTGATGAATCAAATCGATCTTTTCAATAACTACACTAAAAGGCTTCAAAAAATTGTGGGTGCAGGTCAAAGTAGAAGGATTATTAAGGATGCTTTTGTTGTGATTAGTGCAGGAACCAATGACTTTAACGTCAACTTTTATAACATTCCCACAAGGCAGCTACAGTACAATATTAGTGGTTACCAAGATTTTATACAGAATAAGCTACAAAGCTTGATCAAGGTACATTATACAAAATGTTTATGTACACATCATGTGGGCGTGCCGGAGTGGTTATCGGGCATGATTAGAAATCATGTGGGCTTTGCCAGTGCAGGTTTGAATCCTGCCGCTCACGCTTTGTTTTTGTATTTTTTTTCCTTTTAATTTATGAACATCTGTTCAAAGATTTTTTTTTCCAAATGACCTAATTCTACTTGTGTTAATGTAGAATGCCCCAAATCTTAAAAACAATGAAAATTAACCTTATGCTTACATCATCACTGAACAATGTGGAACTACCTCTAGCAATTGCAAGTTCTCACCCATCGCAAATTATTTTGATCACTGACGCGATTTCAAAGTGCATCACGATTTTTTTTTTTAAATCTCTCTGTTGGCCATACACGATTGCTAGTGCCCAACCCCCACAAAGTGATAGCGGAGTTCGATTACGATCTTAAGGCGAATTGTTATTTGTAAAAAGTTCATACTTTACACAGCCCTCTGTTATAATTTCGTGATGGGGAGAACTCGTGACTATTTTGAACCCAACTCAATCCAAATGAGTTGATAACCCAACCAAAACCGTGCCGGTTGGGTAGCTCAAATTTTTTTTTCAGGTCATTAGACTTTTTGAACATCCCTAATCGACGATATAATGTCTTAACCAATTGAGCTATACTCAAAGTGACATATATGGTTGCACTTGAAGAAACTTTGATAGAGACATGGATCGATAAAATTTTATGCAAACATGAGTATCGTTACTTGAGGACCGAATGGAAATTAGGTTCAAAGTTTCAAAAGACCGAACGGGTATTTTCTCTTAAATAATACTAAGATGGAGAAGTATATCATCAATTAAGGATGTCAAAGTGTACAACATGTGGGCGTGCCGGAGTGGTTATCGGGCATGACTAGAAATCATGTGGGCTTTGCCCGCGCAGGTTCGAATCCTGCCGCTCACGCTTTTTCGCTTTTTTTTTTTTTTTGCATCTTTTTTCTCATATTTTGGTGATTCTAAGCTCCTCCAATCTGACATCCCTTATACCTGGAGTCATTCCTGTGATGAAGCAAATCTATCTTTGTAAGAACTACATAGGGCCATTCGTTTTTTGGGAATGTAGATAACTGGAATCTTAGATGTCTGGAATTATAGATATACGGAATTATAGATGTCCGACATCTTTAGATTACCATGTTTGTTTTGTAGGAATATTTTTGCAGATATCTAGAAATATTTTGATAATTGCGTTTGTTTCGTAAGAATTCTACTAGAAAATGTCTTAAATTTACATAATGTTCCAAGAATGCCCTTATACAACATAAAGAAATGTAGGAGCCATATGGAAACTAAATTCAAAAGGCAAGGTAAAATGGTAACATTTTGAAAACTAATGATCAAATGAAAACTAAACTCAAAACTTAGTAGTAAAAATTTGACAATTGGAAACTTAGGGACTTGGAGAACCAATACTCAAAACTAATGAATAAATAATATATGTTCATTTTTTAATCATCACTTATCGACGATATAAATGTCTTAACCAATTGTGCAACGCTCAAGATGACATATATGGTTGCACTTGAAGAAACTTCGTTAGAGATATAGAATGATAAAACTCTATGCAATCATGAGTATAGTTAGGGTTACTTGAGGATCGAATGAAAATTAGATTCAAAGTTTTGGGACCAAACATGTATAGTTTCTTAAATAATAATAAGATGGAAGATTATATTATCGGTTAAGGATGTTTATGTACACATCATGTGGGCGTGCCGGGGTGGTTATTGGGCATGACTAGAAATCATGTGGGCTTTGCCCAAATGTAAAATGAGGATGCCCTGAACTTAAGAATCTGGAAAACTCATGGTTTAGGAGCGCATCCAAAACTGGATGCCTAAGAATCTCCAGACGTCAGGGCATCTTATAATACCGCAAAACAAACATAGTAATCTAGATACTCATTCCCTAGGAATCTAGGACGCCTGCAAAACAAACGGCCCCTAAAAGGCTTCAAAAGATTGTGGGTATGCATCAAAGTAGGAGGATTCTTAACAATGCTTTTGTTGTGATTAGTGCAGGAACCAATGACTTTAACATCAATATCTATGACCTTCGCCTAAGGCAGCTACAGTACAATATTAGTGGTTACCAAGATTTTATACAGAATAAGCTACAAAGCTTGATCAACGTAAATTATACAATAGGCACACCATTAATATCCTAATCAATATTTAACATAGAATAATAAAAAGATCAACACTCCGTCTCATATCCATTTAGTTTGTTGTTTTTGAAATTTGTGTTTTTTCTCACAAATTCTTTATAATCTCTTAGTTGGGTCTCCCTTATTTTAGTCGAGTTTTTGTAGGCTTGATTTTTTTTTTTTTTGAATATCATTGTATTCTTTCCCCCGTTAGTCTATACTTGTACCATCAAAACAAACACTTCAACTCTACTGAAAATAAACACTTTTAACTCTGTTTTCAATTGAAAATGGACAGGAAATTTACCAGCTTGGATGTCGCAATATGGTGGTGGCTGGCCTTCCTCCAATTGGTTGTCTTCCCATTCAACATACCTTATCAATTCGGCATCTCTTCAATCGAAAATGTGTGGAACAACAAAACACAGATTCCAGAGTCTACAATCAGAAGCTTTCAAAGCTACTAAGGAATTTACAGCCACAGCTTCCTAGCAGCAAAATCCTAAGAAATATGTCAAGTCTCAGTCAGTGGTACTTAAAATTGAATCTAATGCAGTAAATATGTGAAGGGAAATGCAGTTTTGAGAGCCATTTTGAACTAAATTTGGTTGGGGCATTTTGTCGAACAGGTTTTGAGCATACCAATAAAGGTTGATGTGGAACTGTATTGAAGGAAGCAGGGCCTCTCTGTAACCCAATAACTCCAAAACGCATCAAAATTTGTGTTCTATGATAGCATTCATCCCACTGAAGCAGCCTACGAGGTGATTGCGGATTCGGTTTGTATCGGAATTAACGGCTGCGGTCGAGTTCTTCGTTCTGAGGCTCTTGGGAATGAAAATGTTGAGGTGATTTTGAACTACAATGTTTTATTCTCTGATTCTGCCAGATTTCTTTCAACTCTCCATTGA

mRNA sequence

ATGGCACGAACTAATAATTACCTCATTGCAGCTCTATCATTCCACATAATTTGGCTGCTATTTCTTAGCAAACCAGGAAGTGCTTTAAAGCCTAAAAGTCCTCGGTCATTCCTTGCCATTCTCATATTTGGTGATTCTACCGTAGATACCGGAAATAACAACTTTATTTCCATCCTTTTCAAAGCCAATTACTTTCCATACGGCCAAGATTTTCCTGGCCATGTTAGTAGTGGAAGGTTTAGTGATGGAAAAATCATTCCTGATATGGTGGCTTCTAGGTTAGGGATAAAGGAGTTTGTTCCTCCATTTTTGGATCCGAAATTGTCGAATGATGATGTGAAAACAGGGGTTAGTTTTGCCTCGGCTGGCACTGGCTTCGATGATCTGACAGCCGCTATATCTAGAGTCATTGCTGTGATGAATCAAATCGATCTTTTCAATAACTACACTAAAAGGCTTCAAAAAATTGTGGGTGCAGGTCAAAGTAGAAGGATTATTAAGGATGCTTTTGTTGTGATTAGTGCAGGAACCAATGACTTTAACGTCAACTTTTATAACATTCCCACAAGGCAGCTACAGTACAATATTAGTGGTTACCAAGATTTTATACAGAATAAGCTACAAAGCTTGATCAAGGAAATTTACCAGCTTGGATGTCGCAATATGGTGGTGGCTGGCCTTCCTCCAATTGGTTGTCTTCCCATTCAACATACCTTATCAATTCGGCATCTCTTCAATCGAAAATGTGTGGAACAACAAAACACAGATTCCAGAGTCTACAATCAGAAGCTTTCAAAGCTACTAAGGAATTTACAGCCACAGCTTCCTAGCAGCAAAATCCTAAGAAATATGTCAAGTCTCACCTACGAGGTGATTGCGGATTCGGTTTGTATCGGAATTAACGGCTGCGGTCGAGTTCTTCGTTCTGAGGCTCTTGGGAATGAAAATGTTGAGGTGATTTTGAACTACAATGTTTTATTCTCTGATTCTGCCAGATTTCTTTCAACTCTCCATTGA

Coding sequence (CDS)

ATGGCACGAACTAATAATTACCTCATTGCAGCTCTATCATTCCACATAATTTGGCTGCTATTTCTTAGCAAACCAGGAAGTGCTTTAAAGCCTAAAAGTCCTCGGTCATTCCTTGCCATTCTCATATTTGGTGATTCTACCGTAGATACCGGAAATAACAACTTTATTTCCATCCTTTTCAAAGCCAATTACTTTCCATACGGCCAAGATTTTCCTGGCCATGTTAGTAGTGGAAGGTTTAGTGATGGAAAAATCATTCCTGATATGGTGGCTTCTAGGTTAGGGATAAAGGAGTTTGTTCCTCCATTTTTGGATCCGAAATTGTCGAATGATGATGTGAAAACAGGGGTTAGTTTTGCCTCGGCTGGCACTGGCTTCGATGATCTGACAGCCGCTATATCTAGAGTCATTGCTGTGATGAATCAAATCGATCTTTTCAATAACTACACTAAAAGGCTTCAAAAAATTGTGGGTGCAGGTCAAAGTAGAAGGATTATTAAGGATGCTTTTGTTGTGATTAGTGCAGGAACCAATGACTTTAACGTCAACTTTTATAACATTCCCACAAGGCAGCTACAGTACAATATTAGTGGTTACCAAGATTTTATACAGAATAAGCTACAAAGCTTGATCAAGGAAATTTACCAGCTTGGATGTCGCAATATGGTGGTGGCTGGCCTTCCTCCAATTGGTTGTCTTCCCATTCAACATACCTTATCAATTCGGCATCTCTTCAATCGAAAATGTGTGGAACAACAAAACACAGATTCCAGAGTCTACAATCAGAAGCTTTCAAAGCTACTAAGGAATTTACAGCCACAGCTTCCTAGCAGCAAAATCCTAAGAAATATGTCAAGTCTCACCTACGAGGTGATTGCGGATTCGGTTTGTATCGGAATTAACGGCTGCGGTCGAGTTCTTCGTTCTGAGGCTCTTGGGAATGAAAATGTTGAGGTGATTTTGAACTACAATGTTTTATTCTCTGATTCTGCCAGATTTCTTTCAACTCTCCATTGA

Protein sequence

MARTNNYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILFKANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKILRNMSSLTYEVIADSVCIGINGCGRVLRSEALGNENVEVILNYNVLFSDSARFLSTLH
Homology
BLAST of HG10006386 vs. NCBI nr
Match: XP_004143225.1 (GDSL esterase/lipase At2g30310 [Cucumis sativus])

HSP 1 Score: 429.9 bits (1104), Expect = 2.1e-116
Identity = 213/281 (75.80%), Postives = 242/281 (86.12%), Query Frame = 0

Query: 1   MARTNNYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILF 60
           MAR  NYLIAALS HIIWLLFLSKP SAL PK+ RSF ++LIFGDSTVDTGNNNFI  +F
Sbjct: 1   MARA-NYLIAALSIHIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIF 60

Query: 61  KANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY+PYG+DFPGHV++GRFSDGK+IPDMVAS+LGIKE VPPFLDP+LS+DDVKTGVSFA
Sbjct: 61  KANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFA 120

Query: 121 SAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDF 180
           SAGTG DDLTAAIS+VI  M QID+F NY +RLQ+IVG  +S+RII  A  VIS GTND 
Sbjct: 121 SAGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDL 180

Query: 181 NVNFYNIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLS 240
             NFY+IPTRQLQYNISGYQ+F+QN+LQSLIKEIYQLGCR +VVAGLPPIGCLPIQ T+S
Sbjct: 181 TFNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 240

Query: 241 IRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
                NR+C+E QN D+  YNQKLSKLL +LQPQLP S+IL
Sbjct: 241 SPIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSQIL 280

BLAST of HG10006386 vs. NCBI nr
Match: KAE8646939.1 (hypothetical protein Csa_021052 [Cucumis sativus])

HSP 1 Score: 429.9 bits (1104), Expect = 2.1e-116
Identity = 213/281 (75.80%), Postives = 242/281 (86.12%), Query Frame = 0

Query: 1   MARTNNYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILF 60
           MAR  NYLIAALS HIIWLLFLSKP SAL PK+ RSF ++LIFGDSTVDTGNNNFI  +F
Sbjct: 1   MARA-NYLIAALSIHIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIF 60

Query: 61  KANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY+PYG+DFPGHV++GRFSDGK+IPDMVAS+LGIKE VPPFLDP+LS+DDVKTGVSFA
Sbjct: 61  KANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFA 120

Query: 121 SAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDF 180
           SAGTG DDLTAAIS+VI  M QID+F NY +RLQ+IVG  +S+RII  A  VIS GTND 
Sbjct: 121 SAGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDL 180

Query: 181 NVNFYNIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLS 240
             NFY+IPTRQLQYNISGYQ+F+QN+LQSLIKEIYQLGCR +VVAGLPPIGCLPIQ T+S
Sbjct: 181 TFNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 240

Query: 241 IRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
                NR+C+E QN D+  YNQKLSKLL +LQPQLP S+IL
Sbjct: 241 SPIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSQIL 280

BLAST of HG10006386 vs. NCBI nr
Match: XP_016902930.1 (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0066338.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK00940.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 427.9 bits (1099), Expect = 7.9e-116
Identity = 208/276 (75.36%), Postives = 239/276 (86.59%), Query Frame = 0

Query: 6   NYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILFKANYF 65
           N LIA LS H IWLL L+KP S+L+PK+  SF AILIFGDSTVDTGNNNFI  +FK NY 
Sbjct: 5   NCLIATLSLHTIWLLLLTKPCSSLEPKTSSSFPAILIFGDSTVDTGNNNFIPTIFKGNYS 64

Query: 66  PYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTG 125
           PYG++FPGH+++GRFSDGK+IPDMVASRLGIKE VPPFLDPKLSNDD+KTGVSFASAGTG
Sbjct: 65  PYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFASAGTG 124

Query: 126 FDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDFNVNFY 185
           FDDLTAAIS+VI VM QID F NY +RLQ +VG G+S+RII +A VVISAGTND N+NFY
Sbjct: 125 FDDLTAAISKVIPVMKQIDHFKNYIQRLQGLVGVGESKRIISNALVVISAGTNDLNINFY 184

Query: 186 NIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLSIRHLF 245
           ++PTRQLQYNISGYQDF+QN+L+SLIKEIYQLGCRN+VVAGLPP+GCLPIQ T++  +  
Sbjct: 185 DLPTRQLQYNISGYQDFLQNRLKSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIAFENPL 244

Query: 246 NRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
            R C+E QN+DS  YNQKLSKLL NLQPQL  SKIL
Sbjct: 245 KRHCLEDQNSDSIAYNQKLSKLLTNLQPQLSGSKIL 280

BLAST of HG10006386 vs. NCBI nr
Match: XP_008463828.1 (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0059943.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07588.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 425.2 bits (1092), Expect = 5.1e-115
Identity = 213/281 (75.80%), Postives = 238/281 (84.70%), Query Frame = 0

Query: 1   MARTNNYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILF 60
           MAR  NYLI ALS HIIWLL LSKP SAL PK+ RSF AILIFGDSTVDTGNNNFIS +F
Sbjct: 1   MARA-NYLITALSIHIIWLLLLSKPCSALAPKTSRSFPAILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY+PYG+DFPG V++GRFSDGK+IPDMVAS+LGIKE VPPFLDPKLS+DDVKTGVSFA
Sbjct: 61  KANYWPYGKDFPGQVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSDDDVKTGVSFA 120

Query: 121 SAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDF 180
           SAGTG DDLTAAIS+VI  M QID+F NY +RLQ+I G  +S+RII  A  VIS GTND 
Sbjct: 121 SAGTGVDDLTAAISKVIPTMKQIDMFKNYIQRLQRIAGVDESKRIIGSALTVISVGTNDL 180

Query: 181 NVNFYNIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLS 240
             NFY+IPTRQLQYNISGYQ+F+QN+LQSLIKEIYQLGCR +VVAGLPPIGCLPIQ T+S
Sbjct: 181 TYNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 240

Query: 241 IRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
                NR+C+E QN D+  YNQKLSKLL +LQPQLP S IL
Sbjct: 241 SPIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSTIL 280

BLAST of HG10006386 vs. NCBI nr
Match: XP_008463830.1 (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0059942.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07587.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 424.5 bits (1090), Expect = 8.7e-115
Identity = 206/281 (73.31%), Postives = 245/281 (87.19%), Query Frame = 0

Query: 1   MARTNNYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILF 60
           MAR   Y IAALS H+IWLLFLSKP +AL+PK  RSF +ILIFGDSTVDTGNNNFIS +F
Sbjct: 1   MARA-TYHIAALSLHMIWLLFLSKPCTALEPKITRSFPSILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY PYG DFPGHV++GRFSDGK+IPDMVAS+LGIKE VPPFLD KLS+D+VKTGVSFA
Sbjct: 61  KANYSPYGADFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDTKLSDDNVKTGVSFA 120

Query: 121 SAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDF 180
           SAG+GFD+LTA++S VI VM QID+F NY +RLQ IVG  +SR+I+  A V+ISAGTND 
Sbjct: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYIRRLQGIVGVDESRKILNSALVIISAGTNDV 180

Query: 181 NVNFYNIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLS 240
           N+NFY++PTRQLQYNISGYQDF+Q++LQSLIKEIYQLGCR MVVAGLPP+GCLPIQ +++
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQDRLQSLIKEIYQLGCRTMVVAGLPPVGCLPIQESIA 240

Query: 241 IRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
            ++  +RKC+E+QN+DS+ YNQKL+ LL NLQPQLP S IL
Sbjct: 241 FQNPLDRKCLEEQNSDSKAYNQKLAHLLSNLQPQLPGSTIL 280

BLAST of HG10006386 vs. ExPASy Swiss-Prot
Match: O22927 (GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.0e-75
Identity = 142/247 (57.49%), Postives = 182/247 (73.68%), Query Frame = 0

Query: 37  FLAILIFGDSTVDTGNNNFIS-ILFKANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLG 96
           F AILIFGDSTVDTGNNN+ S  +FKA + PYG D PGH ++GR+S+GK+I D++AS+L 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLN 91

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQK 156
           IKE VPPFL P +S+ D+ TGVSFASAG G+DD ++  S+ I V  Q  +F NY  RL+ 
Sbjct: 92  IKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKG 151

Query: 157 IVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTRQLQY-NISGYQDFIQNKLQSLIKEI 216
           IVG  ++  II +A VVISAG NDF +NFY+IPTR+L+Y  I GYQ+FI  +L   ++E+
Sbjct: 152 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVREL 211

Query: 217 YQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQ 276
           Y LGCRN+VV GLPP+GCLPIQ T  +R++  R CVEQ+N DS +YNQKL K L  +Q  
Sbjct: 212 YSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYNQKLVKKLPEIQAS 271

Query: 277 LPSSKIL 282
           LP S  L
Sbjct: 272 LPGSNFL 277

BLAST of HG10006386 vs. ExPASy Swiss-Prot
Match: O22918 (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.5e-74
Identity = 140/247 (56.68%), Postives = 180/247 (72.87%), Query Frame = 0

Query: 37  FLAILIFGDSTVDTGNNNFIS-ILFKANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLG 96
           F AILIFGDST DTGNNN+ S  +FKAN+ PYG D PGH ++GRFS+GK+I D+++++L 
Sbjct: 31  FPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLN 90

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQK 156
           IKEFVPPFL P +S+ D+ TGV FASAG G+DD T+  S+ I V  Q  +F NY  RL+ 
Sbjct: 91  IKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKG 150

Query: 157 IVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTRQLQY-NISGYQDFIQNKLQSLIKEI 216
           IVG  ++  II +A VVISAG NDF +NFY+IP R+L+Y  I GYQDF+  +L   ++E+
Sbjct: 151 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVREL 210

Query: 217 YQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQ 276
           Y LGCRN++V GLPP+GCLPIQ T  +R +    CVEQ+N DS +YNQKL K L  +Q  
Sbjct: 211 YSLGCRNILVGGLPPMGCLPIQLTAKLRTILG-ICVEQENKDSILYNQKLVKKLPEIQAS 270

Query: 277 LPSSKIL 282
           LP SK L
Sbjct: 271 LPGSKFL 276

BLAST of HG10006386 vs. ExPASy Swiss-Prot
Match: Q9SJA9 (GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2 SV=2)

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-73
Identity = 141/272 (51.84%), Postives = 193/272 (70.96%), Query Frame = 0

Query: 12  LSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFIS-ILFKANYFPYGQD 71
           ++F +     LS   +A    S   F AILIFGDSTVDTGNNN+ S  +FKA + PYG D
Sbjct: 7   ITFTLFIAALLSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGID 66

Query: 72  FPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLT 131
            P H +SGRF++GKI  D++A++L IK+FVPPFL P LS+ ++ TGV FASAG G+DD T
Sbjct: 67  LPNHKASGRFTNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHT 126

Query: 132 AAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTR 191
           +  ++ I V++Q  +F NY  RL+ IVG  ++  IIK+A VVISAG NDF +N+Y+IP+R
Sbjct: 127 SLSTQAIRVLDQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSR 186

Query: 192 QLQY-NISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKC 251
           +L++ +ISGYQDF+  +L + ++E+Y LGCR ++V GLPP+GCLPIQ T   R+   R C
Sbjct: 187 RLEFPHISGYQDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAKFRNAL-RFC 246

Query: 252 VEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
           +EQ+N DS +YNQKL  LL  ++  L  SKIL
Sbjct: 247 LEQENRDSVLYNQKLQNLLPQIEASLTGSKIL 277

BLAST of HG10006386 vs. ExPASy Swiss-Prot
Match: Q9SIQ2 (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 275.8 bits (704), Expect = 6.6e-73
Identity = 135/247 (54.66%), Postives = 185/247 (74.90%), Query Frame = 0

Query: 37  FLAILIFGDSTVDTGNNNF-ISILFKANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLG 96
           F AILIFGDSTVDTGNNN+ +  +F+A +FPYG D P   ++GRFS+GK+I D++A++L 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQK 156
           IKEF+PPFL P LS+ D+ TGV FASAG G+DDLT+  ++ I V  Q ++F +Y  RL+ 
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 157 IVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTRQLQYN-ISGYQDFIQNKLQSLIKEI 216
           IVG  ++  II +AFVV+SAG NDF +N+Y+IP+R+L+Y  ISGYQDFI  +L++ ++E+
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYDIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 217 YQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQ 276
           Y LG RN++V GLPP+GCLPI  T   R++F R C+E  N DS +YN+KL KLL  ++  
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFRNIF-RFCLEHHNKDSVLYNEKLQKLLPQIEAS 272

Query: 277 LPSSKIL 282
           LP SK L
Sbjct: 273 LPGSKFL 278

BLAST of HG10006386 vs. ExPASy Swiss-Prot
Match: Q9SIQ3 (GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.3e-72
Identity = 134/247 (54.25%), Postives = 183/247 (74.09%), Query Frame = 0

Query: 37  FLAILIFGDSTVDTGNNNF-ISILFKANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLG 96
           F AILIFGDSTVDTGNNN+ +  +F+A +FPYG D P   ++GRFS+GK+I D++A++L 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQK 156
           IKEF+PPFL P LS+ D+ TGV FASAG G+DDLT+  ++ I V  Q ++F +Y  RL+ 
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 157 IVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTRQLQYN-ISGYQDFIQNKLQSLIKEI 216
           IVG  ++  II +AFVV+SAG NDF +N+Y IP+R+L+Y  ISGYQDFI  +L++ ++E+
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 217 YQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQ 276
           Y LG RN++V GLPP+GCLPI  T   R++F R C+E  N DS +YN+KL  LL  ++  
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFRNIF-RFCLEHHNKDSVLYNEKLQNLLPQIEAS 272

Query: 277 LPSSKIL 282
           LP SK L
Sbjct: 273 LPGSKFL 278

BLAST of HG10006386 vs. ExPASy TrEMBL
Match: A0A0A0KF79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G180980 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 1.0e-116
Identity = 213/281 (75.80%), Postives = 242/281 (86.12%), Query Frame = 0

Query: 1   MARTNNYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILF 60
           MAR  NYLIAALS HIIWLLFLSKP SAL PK+ RSF ++LIFGDSTVDTGNNNFI  +F
Sbjct: 1   MARA-NYLIAALSIHIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIF 60

Query: 61  KANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY+PYG+DFPGHV++GRFSDGK+IPDMVAS+LGIKE VPPFLDP+LS+DDVKTGVSFA
Sbjct: 61  KANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFA 120

Query: 121 SAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDF 180
           SAGTG DDLTAAIS+VI  M QID+F NY +RLQ+IVG  +S+RII  A  VIS GTND 
Sbjct: 121 SAGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDL 180

Query: 181 NVNFYNIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLS 240
             NFY+IPTRQLQYNISGYQ+F+QN+LQSLIKEIYQLGCR +VVAGLPPIGCLPIQ T+S
Sbjct: 181 TFNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 240

Query: 241 IRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
                NR+C+E QN D+  YNQKLSKLL +LQPQLP S+IL
Sbjct: 241 SPIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSQIL 280

BLAST of HG10006386 vs. ExPASy TrEMBL
Match: A0A5A7VF47 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001150 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 3.8e-116
Identity = 208/276 (75.36%), Postives = 239/276 (86.59%), Query Frame = 0

Query: 6   NYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILFKANYF 65
           N LIA LS H IWLL L+KP S+L+PK+  SF AILIFGDSTVDTGNNNFI  +FK NY 
Sbjct: 5   NCLIATLSLHTIWLLLLTKPCSSLEPKTSSSFPAILIFGDSTVDTGNNNFIPTIFKGNYS 64

Query: 66  PYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTG 125
           PYG++FPGH+++GRFSDGK+IPDMVASRLGIKE VPPFLDPKLSNDD+KTGVSFASAGTG
Sbjct: 65  PYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFASAGTG 124

Query: 126 FDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDFNVNFY 185
           FDDLTAAIS+VI VM QID F NY +RLQ +VG G+S+RII +A VVISAGTND N+NFY
Sbjct: 125 FDDLTAAISKVIPVMKQIDHFKNYIQRLQGLVGVGESKRIISNALVVISAGTNDLNINFY 184

Query: 186 NIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLSIRHLF 245
           ++PTRQLQYNISGYQDF+QN+L+SLIKEIYQLGCRN+VVAGLPP+GCLPIQ T++  +  
Sbjct: 185 DLPTRQLQYNISGYQDFLQNRLKSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIAFENPL 244

Query: 246 NRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
            R C+E QN+DS  YNQKLSKLL NLQPQL  SKIL
Sbjct: 245 KRHCLEDQNSDSIAYNQKLSKLLTNLQPQLSGSKIL 280

BLAST of HG10006386 vs. ExPASy TrEMBL
Match: A0A1S4E4N0 (GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501132 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 3.8e-116
Identity = 208/276 (75.36%), Postives = 239/276 (86.59%), Query Frame = 0

Query: 6   NYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILFKANYF 65
           N LIA LS H IWLL L+KP S+L+PK+  SF AILIFGDSTVDTGNNNFI  +FK NY 
Sbjct: 5   NCLIATLSLHTIWLLLLTKPCSSLEPKTSSSFPAILIFGDSTVDTGNNNFIPTIFKGNYS 64

Query: 66  PYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTG 125
           PYG++FPGH+++GRFSDGK+IPDMVASRLGIKE VPPFLDPKLSNDD+KTGVSFASAGTG
Sbjct: 65  PYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFASAGTG 124

Query: 126 FDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDFNVNFY 185
           FDDLTAAIS+VI VM QID F NY +RLQ +VG G+S+RII +A VVISAGTND N+NFY
Sbjct: 125 FDDLTAAISKVIPVMKQIDHFKNYIQRLQGLVGVGESKRIISNALVVISAGTNDLNINFY 184

Query: 186 NIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLSIRHLF 245
           ++PTRQLQYNISGYQDF+QN+L+SLIKEIYQLGCRN+VVAGLPP+GCLPIQ T++  +  
Sbjct: 185 DLPTRQLQYNISGYQDFLQNRLKSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIAFENPL 244

Query: 246 NRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
            R C+E QN+DS  YNQKLSKLL NLQPQL  SKIL
Sbjct: 245 KRHCLEDQNSDSIAYNQKLSKLLTNLQPQLSGSKIL 280

BLAST of HG10006386 vs. ExPASy TrEMBL
Match: A0A5D3C8T5 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852G00280 PE=3 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 2.5e-115
Identity = 213/281 (75.80%), Postives = 238/281 (84.70%), Query Frame = 0

Query: 1   MARTNNYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILF 60
           MAR  NYLI ALS HIIWLL LSKP SAL PK+ RSF AILIFGDSTVDTGNNNFIS +F
Sbjct: 1   MARA-NYLITALSIHIIWLLLLSKPCSALAPKTSRSFPAILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY+PYG+DFPG V++GRFSDGK+IPDMVAS+LGIKE VPPFLDPKLS+DDVKTGVSFA
Sbjct: 61  KANYWPYGKDFPGQVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSDDDVKTGVSFA 120

Query: 121 SAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDF 180
           SAGTG DDLTAAIS+VI  M QID+F NY +RLQ+I G  +S+RII  A  VIS GTND 
Sbjct: 121 SAGTGVDDLTAAISKVIPTMKQIDMFKNYIQRLQRIAGVDESKRIIGSALTVISVGTNDL 180

Query: 181 NVNFYNIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLS 240
             NFY+IPTRQLQYNISGYQ+F+QN+LQSLIKEIYQLGCR +VVAGLPPIGCLPIQ T+S
Sbjct: 181 TYNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 240

Query: 241 IRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
                NR+C+E QN D+  YNQKLSKLL +LQPQLP S IL
Sbjct: 241 SPIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSTIL 280

BLAST of HG10006386 vs. ExPASy TrEMBL
Match: A0A1S3CK41 (GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501877 PE=3 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 2.5e-115
Identity = 213/281 (75.80%), Postives = 238/281 (84.70%), Query Frame = 0

Query: 1   MARTNNYLIAALSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFISILF 60
           MAR  NYLI ALS HIIWLL LSKP SAL PK+ RSF AILIFGDSTVDTGNNNFIS +F
Sbjct: 1   MARA-NYLITALSIHIIWLLLLSKPCSALAPKTSRSFPAILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY+PYG+DFPG V++GRFSDGK+IPDMVAS+LGIKE VPPFLDPKLS+DDVKTGVSFA
Sbjct: 61  KANYWPYGKDFPGQVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSDDDVKTGVSFA 120

Query: 121 SAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDF 180
           SAGTG DDLTAAIS+VI  M QID+F NY +RLQ+I G  +S+RII  A  VIS GTND 
Sbjct: 121 SAGTGVDDLTAAISKVIPTMKQIDMFKNYIQRLQRIAGVDESKRIIGSALTVISVGTNDL 180

Query: 181 NVNFYNIPTRQLQYNISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLS 240
             NFY+IPTRQLQYNISGYQ+F+QN+LQSLIKEIYQLGCR +VVAGLPPIGCLPIQ T+S
Sbjct: 181 TYNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 240

Query: 241 IRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
                NR+C+E QN D+  YNQKLSKLL +LQPQLP S IL
Sbjct: 241 SPIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSTIL 280

BLAST of HG10006386 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.0e-76
Identity = 142/247 (57.49%), Postives = 182/247 (73.68%), Query Frame = 0

Query: 37  FLAILIFGDSTVDTGNNNFIS-ILFKANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLG 96
           F AILIFGDSTVDTGNNN+ S  +FKA + PYG D PGH ++GR+S+GK+I D++AS+L 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLN 91

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQK 156
           IKE VPPFL P +S+ D+ TGVSFASAG G+DD ++  S+ I V  Q  +F NY  RL+ 
Sbjct: 92  IKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKG 151

Query: 157 IVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTRQLQY-NISGYQDFIQNKLQSLIKEI 216
           IVG  ++  II +A VVISAG NDF +NFY+IPTR+L+Y  I GYQ+FI  +L   ++E+
Sbjct: 152 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVREL 211

Query: 217 YQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQ 276
           Y LGCRN+VV GLPP+GCLPIQ T  +R++  R CVEQ+N DS +YNQKL K L  +Q  
Sbjct: 212 YSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYNQKLVKKLPEIQAS 271

Query: 277 LPSSKIL 282
           LP S  L
Sbjct: 272 LPGSNFL 277

BLAST of HG10006386 vs. TAIR 10
Match: AT2G30220.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.2e-75
Identity = 140/247 (56.68%), Postives = 180/247 (72.87%), Query Frame = 0

Query: 37  FLAILIFGDSTVDTGNNNFIS-ILFKANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLG 96
           F AILIFGDST DTGNNN+ S  +FKAN+ PYG D PGH ++GRFS+GK+I D+++++L 
Sbjct: 31  FPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLN 90

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQK 156
           IKEFVPPFL P +S+ D+ TGV FASAG G+DD T+  S+ I V  Q  +F NY  RL+ 
Sbjct: 91  IKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKG 150

Query: 157 IVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTRQLQY-NISGYQDFIQNKLQSLIKEI 216
           IVG  ++  II +A VVISAG NDF +NFY+IP R+L+Y  I GYQDF+  +L   ++E+
Sbjct: 151 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVREL 210

Query: 217 YQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQ 276
           Y LGCRN++V GLPP+GCLPIQ T  +R +    CVEQ+N DS +YNQKL K L  +Q  
Sbjct: 211 YSLGCRNILVGGLPPMGCLPIQLTAKLRTILG-ICVEQENKDSILYNQKLVKKLPEIQAS 270

Query: 277 LPSSKIL 282
           LP SK L
Sbjct: 271 LPGSKFL 276

BLAST of HG10006386 vs. TAIR 10
Match: AT2G24560.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.2e-74
Identity = 141/272 (51.84%), Postives = 193/272 (70.96%), Query Frame = 0

Query: 12  LSFHIIWLLFLSKPGSALKPKSPRSFLAILIFGDSTVDTGNNNFIS-ILFKANYFPYGQD 71
           ++F +     LS   +A    S   F AILIFGDSTVDTGNNN+ S  +FKA + PYG D
Sbjct: 7   ITFTLFIAALLSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGID 66

Query: 72  FPGHVSSGRFSDGKIIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLT 131
            P H +SGRF++GKI  D++A++L IK+FVPPFL P LS+ ++ TGV FASAG G+DD T
Sbjct: 67  LPNHKASGRFTNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHT 126

Query: 132 AAISRVIAVMNQIDLFNNYTKRLQKIVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTR 191
           +  ++ I V++Q  +F NY  RL+ IVG  ++  IIK+A VVISAG NDF +N+Y+IP+R
Sbjct: 127 SLSTQAIRVLDQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSR 186

Query: 192 QLQY-NISGYQDFIQNKLQSLIKEIYQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKC 251
           +L++ +ISGYQDF+  +L + ++E+Y LGCR ++V GLPP+GCLPIQ T   R+   R C
Sbjct: 187 RLEFPHISGYQDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAKFRNAL-RFC 246

Query: 252 VEQQNTDSRVYNQKLSKLLRNLQPQLPSSKIL 282
           +EQ+N DS +YNQKL  LL  ++  L  SKIL
Sbjct: 247 LEQENRDSVLYNQKLQNLLPQIEASLTGSKIL 277

BLAST of HG10006386 vs. TAIR 10
Match: AT2G31540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 2.3e-73
Identity = 134/247 (54.25%), Postives = 183/247 (74.09%), Query Frame = 0

Query: 37  FLAILIFGDSTVDTGNNNF-ISILFKANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLG 96
           F AILIFGDSTVDTGNNN+ +  +F+A +FPYG D P   ++GRFS+GK+I D++A++L 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQK 156
           IKEF+PPFL P LS+ D+ TGV FASAG G+DDLT+  ++ I V  Q ++F +Y  RL+ 
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 157 IVGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTRQLQYN-ISGYQDFIQNKLQSLIKEI 216
           IVG  ++  II +AFVV+SAG NDF +N+Y IP+R+L+Y  ISGYQDFI  +L++ ++E+
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 217 YQLGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQ 276
           Y LG RN++V GLPP+GCLPI  T   R++F R C+E  N DS +YN+KL  LL  ++  
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFRNIF-RFCLEHHNKDSVLYNEKLQNLLPQIEAS 272

Query: 277 LPSSKIL 282
           LP SK L
Sbjct: 273 LPGSKFL 278

BLAST of HG10006386 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-72
Identity = 121/244 (49.59%), Postives = 181/244 (74.18%), Query Frame = 0

Query: 37  FLAILIFGDSTVDTGNNNFISILFKANYFPYGQDFPGHVSSGRFSDGKIIPDMVASRLGI 96
           F AIL+FGDST+DTGNNN+I    +AN+ PYG +FPGH ++GRFS+GK+IPD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 97  KEFVPPFLDPKLSNDDVKTGVSFASAGTGFDDLTAAISRVIAVMNQIDLFNNYTKRLQKI 156
           K+ VPPFLDP LS+ D+ TGV FASAG+G+D+LT   +  ++V  Q D+  +Y +RL +I
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 157 VGAGQSRRIIKDAFVVISAGTNDFNVNFYNIPTRQLQYNISGYQDFIQNKLQSLIKEIYQ 216
           VG  ++  I+ +A V++S+GTNDFN+N Y+ P+R+ +  + GYQ FI + + + ++E+Y 
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYD 214

Query: 217 LGCRNMVVAGLPPIGCLPIQHTLSIRHLFNRKCVEQQNTDSRVYNQKLSKLLRNLQPQLP 276
           +GCR ++V GLPP+GCLPIQ T++++    R+C+++QN+DS+ +NQKL   L  +Q  L 
Sbjct: 215 IGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLT 274

Query: 277 SSKI 281
            S I
Sbjct: 275 GSVI 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004143225.12.1e-11675.80GDSL esterase/lipase At2g30310 [Cucumis sativus][more]
KAE8646939.12.1e-11675.80hypothetical protein Csa_021052 [Cucumis sativus][more]
XP_016902930.17.9e-11675.36PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0066338.1 GDSL... [more]
XP_008463828.15.1e-11575.80PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0059943.1 GDSL... [more]
XP_008463830.18.7e-11573.31PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0059942.1 GDSL... [more]
Match NameE-valueIdentityDescription
O229277.0e-7557.49GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2... [more]
O229184.5e-7456.68GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3... [more]
Q9SJA91.7e-7351.84GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2... [more]
Q9SIQ26.6e-7354.66GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3... [more]
Q9SIQ33.3e-7254.25GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0KF791.0e-11675.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G180980 PE=3 SV=1[more]
A0A5A7VF473.8e-11675.36GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602... [more]
A0A1S4E4N03.8e-11675.36GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501132 PE=3... [more]
A0A5D3C8T52.5e-11575.80GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852... [more]
A0A1S3CK412.5e-11575.80GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501877 PE=3... [more]
Match NameE-valueIdentityDescription
AT2G30310.15.0e-7657.49GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G30220.13.2e-7556.68GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G24560.11.2e-7451.84GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G31540.12.3e-7354.25GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G06990.11.1e-7249.59GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 28..328
e-value: 7.6E-46
score: 159.0
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 40..274
e-value: 1.1E-22
score: 81.6
NoneNo IPR availablePANTHERPTHR45642:SF30GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 35..282
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 35..282
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 40..234
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 39..280
e-value: 3.06173E-95
score: 283.738

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10006386.1HG10006386.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds