HG10006353 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10006353
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	nicotianamine synthase
Location	Chr07: 17620206 .. 17620610 (-)
RNA-Seq Expression	HG10006353
Synteny	HG10006353

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTGAAGTACCTCCCGAAAGCCGAGTTTCACAACTTCGACGTCGACTCGTCTGCTAATGCCAAAGCAGCGATGCTGGTAGCTGGCGACGCAGACCTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGTTGCCTCGAATGAGTTGAGGCAGTTTCAGGTGGTGTTTTTGGCGGCCTTGGGGGGAGATGAAAAGGAAGAGAAGGTGAAGGTGATTGAGCATTTGAGGAAGAAATTGGGTGGTGGCGCGTTGTTGATGGTGAGGAGCGCATATGGTGCACGCGCGTTTCTTTATCCGGTGGTTGAAGCTTGTGATCTTCGAGGGTTTGAGATTGTTACGGTGTTTCATCCCACGGATGAAGTTATTAATTCAATTGTATTGGCACGATTATAA

mRNA sequence

ATGGCCTTGAAGTACCTCCCGAAAGCCGAGTTTCACAACTTCGACGTCGACTCGTCTGCTAATGCCAAAGCAGCGATGCTGGTAGCTGGCGACGCAGACCTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGTTGCCTCGAATGAGTTGAGGCAGTTTCAGGTGGTGTTTTTGGCGGCCTTGGGGGGAGATGAAAAGGAAGAGAAGGTGAAGGTGATTGAGCATTTGAGGAAGAAATTGGGTGGTGGCGCGTTGTTGATGGTGAGGAGCGCATATGGTGCACGCGCGTTTCTTTATCCGGTGGTTGAAGCTTGTGATCTTCGAGGGTTTGAGATTGTTACGGTGTTTCATCCCACGGATGAAGTTATTAATTCAATTGTATTGGCACGATTATAA

Coding sequence (CDS)

ATGGCCTTGAAGTACCTCCCGAAAGCCGAGTTTCACAACTTCGACGTCGACTCGTCTGCTAATGCCAAAGCAGCGATGCTGGTAGCTGGCGACGCAGACCTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGTTGCCTCGAATGAGTTGAGGCAGTTTCAGGTGGTGTTTTTGGCGGCCTTGGGGGGAGATGAAAAGGAAGAGAAGGTGAAGGTGATTGAGCATTTGAGGAAGAAATTGGGTGGTGGCGCGTTGTTGATGGTGAGGAGCGCATATGGTGCACGCGCGTTTCTTTATCCGGTGGTTGAAGCTTGTGATCTTCGAGGGTTTGAGATTGTTACGGTGTTTCATCCCACGGATGAAGTTATTAATTCAATTGTATTGGCACGATTATAA

Protein sequence

MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVFLAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFHPTDEVINSIVLARL

Homology

[Close]

BLAST of HG10006353 vs. NCBI nr
Match: XP_038891237.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 235.0 bits (598), Expect = 3.9e-58
Identity = 118/134 (88.06%), Postives = 128/134 (95.52%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKYLPK E HNFD+D SANAKAAMLVAGDA+LS+RMVFHTTDIMDVAS+ELR+F+VVF
Sbjct: 144 MALKYLPKTELHNFDIDPSANAKAAMLVAGDAELSKRMVFHTTDIMDVASDELREFEVVF 203

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G E+EEKVKVIEHLRK +GGGALLM+RSAYGARAFLYPVVEACDLRGFEI+TVFH
Sbjct: 204 LAALVGMEREEKVKVIEHLRKSMGGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFH 263

Query: 121 PTDEVINSIVLARL 135
           PTDEVINSIVLARL
Sbjct: 264 PTDEVINSIVLARL 277

[Close]

BLAST of HG10006353 vs. NCBI nr
Match: XP_008453494.1 (PREDICTED: nicotianamine synthase 1-like [Cucumis melo])

HSP 1 Score: 226.9 bits (577), Expect = 1.1e-55
Identity = 114/134 (85.07%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKYLPK EFHNFD+D SANAKA+MLVAGD +LSRRMVFHTTDIM+V S ELR+F+VVF
Sbjct: 146 MALKYLPKTEFHNFDLDPSANAKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVF 205

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G E+EEK KVIEHLRK + GGALLM+RSAYGARAFLYPVVEACDLRGFEI+TVFH
Sbjct: 206 LAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFH 265

Query: 121 PTDEVINSIVLARL 135
           PTDEVINSIVLARL
Sbjct: 266 PTDEVINSIVLARL 279

[Close]

BLAST of HG10006353 vs. NCBI nr
Match: KAA0048068.1 (nicotianamine synthase 1-like [Cucumis melo var. makuwa] >TYJ96468.1 nicotianamine synthase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 226.9 bits (577), Expect = 1.1e-55
Identity = 114/134 (85.07%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKYLPK EFHNFD+D SANAKA+MLVAGD +LSRRMVFHTTDIM+V S ELR+F+VVF
Sbjct: 100 MALKYLPKTEFHNFDLDPSANAKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVF 159

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G E+EEK KVIEHLRK + GGALLM+RSAYGARAFLYPVVEACDLRGFEI+TVFH
Sbjct: 160 LAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFH 219

Query: 121 PTDEVINSIVLARL 135
           PTDEVINSIVLARL
Sbjct: 220 PTDEVINSIVLARL 233

[Close]

BLAST of HG10006353 vs. NCBI nr
Match: XP_004144812.1 (nicotianamine synthase 1 [Cucumis sativus])

HSP 1 Score: 224.2 bits (570), Expect = 6.9e-55
Identity = 112/134 (83.58%), Postives = 123/134 (91.79%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKY PK EFHNFD+D SANAKA+MLVAGD +LSRRMVFHTTDIMDV S ELR+F+VVF
Sbjct: 145 MALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRRMVFHTTDIMDVGSEELREFEVVF 204

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G E+EEK KVIEHLRK + GGALLM+RSAYGARAF+YPVVEACDLRGF+I+TVFH
Sbjct: 205 LAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGARAFVYPVVEACDLRGFDILTVFH 264

Query: 121 PTDEVINSIVLARL 135
           PTDEVINSIVLARL
Sbjct: 265 PTDEVINSIVLARL 278

[Close]

BLAST of HG10006353 vs. NCBI nr
Match: XP_022965879.1 (nicotianamine synthase [Cucurbita maxima])

HSP 1 Score: 216.5 bits (550), Expect = 1.4e-52
Identity = 110/134 (82.09%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKYLP  EFHNFDVDSSANAKA MLVA D DLSRRM FHTTDIMDV S+ LR+F+VVF
Sbjct: 149 MALKYLPTTEFHNFDVDSSANAKATMLVASDVDLSRRMTFHTTDIMDVTSDMLREFEVVF 208

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G EKEEKVKVIEHLRK +  G+LLM+RSAYGARAFLYPVVEA DLRGFEI+T FH
Sbjct: 209 LAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAFLYPVVEASDLRGFEILTTFH 268

Query: 121 PTDEVINSIVLARL 135
           PTD+V+NSIVLARL
Sbjct: 269 PTDDVVNSIVLARL 282

[Close]

BLAST of HG10006353 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.0e-41
Identity = 85/133 (63.91%), Postives = 106/133 (79.70%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           +A  +L  + FHNFD+D SAN  AA LV+ D DLS+RM FHT DIMDV +  L+ F VVF
Sbjct: 143 LASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIMDV-TESLKGFDVVF 202

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G +K+EKVKV+EHL K +  GALLM+RSA+G RAFLYP+VE CDL GFE+++V+H
Sbjct: 203 LAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEPCDLEGFEVLSVYH 262

Query: 121 PTDEVINSIVLAR 134
           PTDEVINSIV++R
Sbjct: 263 PTDEVINSIVISR 274

[Close]

BLAST of HG10006353 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 1.7e-40
Identity = 80/133 (60.15%), Postives = 108/133 (81.20%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           +A  +L    FHNFD+D SAN+ A++LV+ D D+S+RM FHT DIMDV +  L+ F VVF
Sbjct: 142 LASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIMDV-TESLKSFDVVF 201

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G  KEEKVKVIEHL+K +  GA+LM+RSA+G RAFLYP+VE CDL+GFE+++++H
Sbjct: 202 LAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEPCDLQGFEVLSIYH 261

Query: 121 PTDEVINSIVLAR 134
           PTD+VINS+V+++
Sbjct: 262 PTDDVINSVVISK 273

[Close]

BLAST of HG10006353 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 2.9e-40
Identity = 77/133 (57.89%), Postives = 108/133 (81.20%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           +A  +LP   FHNFD+DS AN  A+ LV+ D+DLS+RM+FHTTD+++ A   L Q+ VVF
Sbjct: 142 LAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVLN-AKEGLDQYDVVF 201

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G +KE KVK IEHL K +  GA++M+RSA+G RAFLYP+V++CDL+GFE++T++H
Sbjct: 202 LAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDSCDLKGFEVLTIYH 261

Query: 121 PTDEVINSIVLAR 134
           P+D+V+NS+V+AR
Sbjct: 262 PSDDVVNSVVIAR 273

[Close]

BLAST of HG10006353 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.2e-38
Identity = 76/133 (57.14%), Postives = 106/133 (79.70%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           +A  +LP   FHNFD+DS AN  A+ LV+ D DLS+RM+FHTTD+++ A+  L Q+ VVF
Sbjct: 143 LAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDVLN-ATEGLDQYDVVF 202

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G +KE KVK IEHL K +  GA+LM+RSA+  RAFLYP+V++ DL+GF+++T++H
Sbjct: 203 LAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVDSSDLKGFQLLTIYH 262

Query: 121 PTDEVINSIVLAR 134
           PTD+V+NS+V+AR
Sbjct: 263 PTDDVVNSVVIAR 274

[Close]

BLAST of HG10006353 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 2.7e-38
Identity = 78/133 (58.65%), Postives = 103/133 (77.44%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           +A K+L    FHN+D+D  AN  A+ LVA D D+S RM FHT D+MDV    L+ + VVF
Sbjct: 140 LATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMDVTC-ALKDYDVVF 199

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G +KE+KVKV++HL K +  GA LM+RSA+GARAFLYPV++  DLRGFE+++V+H
Sbjct: 200 LAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPRDLRGFEVLSVYH 259

Query: 121 PTDEVINSIVLAR 134
           PTDEVINS+++AR
Sbjct: 260 PTDEVINSVIIAR 271

[Close]

BLAST of HG10006353 vs. ExPASy TrEMBL
Match: A0A5A7TYM0 (Nicotianamine synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001120 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.1e-56
Identity = 114/134 (85.07%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKYLPK EFHNFD+D SANAKA+MLVAGD +LSRRMVFHTTDIM+V S ELR+F+VVF
Sbjct: 100 MALKYLPKTEFHNFDLDPSANAKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVF 159

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G E+EEK KVIEHLRK + GGALLM+RSAYGARAFLYPVVEACDLRGFEI+TVFH
Sbjct: 160 LAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFH 219

Query: 121 PTDEVINSIVLARL 135
           PTDEVINSIVLARL
Sbjct: 220 PTDEVINSIVLARL 233

[Close]

BLAST of HG10006353 vs. ExPASy TrEMBL
Match: A0A1S3BXJ6 (nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.1e-56
Identity = 114/134 (85.07%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKYLPK EFHNFD+D SANAKA+MLVAGD +LSRRMVFHTTDIM+V S ELR+F+VVF
Sbjct: 146 MALKYLPKTEFHNFDLDPSANAKASMLVAGDVELSRRMVFHTTDIMEVGSEELREFEVVF 205

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G E+EEK KVIEHLRK + GGALLM+RSAYGARAFLYPVVEACDLRGFEI+TVFH
Sbjct: 206 LAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFH 265

Query: 121 PTDEVINSIVLARL 135
           PTDEVINSIVLARL
Sbjct: 266 PTDEVINSIVLARL 279

[Close]

BLAST of HG10006353 vs. ExPASy TrEMBL
Match: A0A0A0LJ09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 3.3e-55
Identity = 112/134 (83.58%), Postives = 123/134 (91.79%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKY PK EFHNFD+D SANAKA+MLVAGD +LSRRMVFHTTDIMDV S ELR+F+VVF
Sbjct: 145 MALKYFPKTEFHNFDLDPSANAKASMLVAGDVELSRRMVFHTTDIMDVGSEELREFEVVF 204

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G E+EEK KVIEHLRK + GGALLM+RSAYGARAF+YPVVEACDLRGF+I+TVFH
Sbjct: 205 LAALVGMEREEKGKVIEHLRKNMSGGALLMLRSAYGARAFVYPVVEACDLRGFDILTVFH 264

Query: 121 PTDEVINSIVLARL 135
           PTDEVINSIVLARL
Sbjct: 265 PTDEVINSIVLARL 278

[Close]

BLAST of HG10006353 vs. ExPASy TrEMBL
Match: A0A6J1HS54 (nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 6.9e-53
Identity = 110/134 (82.09%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKYLP  EFHNFDVDSSANAKA MLVA D DLSRRM FHTTDIMDV S+ LR+F+VVF
Sbjct: 149 MALKYLPTTEFHNFDVDSSANAKATMLVASDVDLSRRMTFHTTDIMDVTSDMLREFEVVF 208

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G EKEEKVKVIEHLRK +  G+LLM+RSAYGARAFLYPVVEA DLRGFEI+T FH
Sbjct: 209 LAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAFLYPVVEASDLRGFEILTTFH 268

Query: 121 PTDEVINSIVLARL 135
           PTD+V+NSIVLARL
Sbjct: 269 PTDDVVNSIVLARL 282

[Close]

BLAST of HG10006353 vs. ExPASy TrEMBL
Match: A0A6J1FHL8 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 3.8e-51
Identity = 108/134 (80.60%), Postives = 117/134 (87.31%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           MALKYLP  EFHNFDVDSSANAKA  LVA D DLSRRM FHTTDIMDV S+ L +F+VVF
Sbjct: 149 MALKYLPTTEFHNFDVDSSANAKATALVASDVDLSRRMSFHTTDIMDVTSDMLGEFEVVF 208

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G EKEEKVKVIEHLRK +  G+LLM+RSAYGARAFLYPVVEA DLRGFEI+T FH
Sbjct: 209 LAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAFLYPVVEASDLRGFEILTTFH 268

Query: 121 PTDEVINSIVLARL 135
           PTD+V+NSIVLARL
Sbjct: 269 PTDDVVNSIVLARL 282

[Close]

BLAST of HG10006353 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 169.9 bits (429), Expect = 1.4e-42
Identity = 85/133 (63.91%), Postives = 106/133 (79.70%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           +A  +L  + FHNFD+D SAN  AA LV+ D DLS+RM FHT DIMDV +  L+ F VVF
Sbjct: 143 LASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIMDV-TESLKGFDVVF 202

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G +K+EKVKV+EHL K +  GALLM+RSA+G RAFLYP+VE CDL GFE+++V+H
Sbjct: 203 LAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEPCDLEGFEVLSVYH 262

Query: 121 PTDEVINSIVLAR 134
           PTDEVINSIV++R
Sbjct: 263 PTDEVINSIVISR 274

[Close]

BLAST of HG10006353 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 166.8 bits (421), Expect = 1.2e-41
Identity = 80/133 (60.15%), Postives = 108/133 (81.20%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           +A  +L    FHNFD+D SAN+ A++LV+ D D+S+RM FHT DIMDV +  L+ F VVF
Sbjct: 142 LASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIMDV-TESLKSFDVVF 201

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G  KEEKVKVIEHL+K +  GA+LM+RSA+G RAFLYP+VE CDL+GFE+++++H
Sbjct: 202 LAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEPCDLQGFEVLSIYH 261

Query: 121 PTDEVINSIVLAR 134
           PTD+VINS+V+++
Sbjct: 262 PTDDVINSVVISK 273

[Close]

BLAST of HG10006353 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 166.0 bits (419), Expect = 2.1e-41
Identity = 77/133 (57.89%), Postives = 108/133 (81.20%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           +A  +LP   FHNFD+DS AN  A+ LV+ D+DLS+RM+FHTTD+++ A   L Q+ VVF
Sbjct: 142 LAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVLN-AKEGLDQYDVVF 201

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G +KE KVK IEHL K +  GA++M+RSA+G RAFLYP+V++CDL+GFE++T++H
Sbjct: 202 LAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDSCDLKGFEVLTIYH 261

Query: 121 PTDEVINSIVLAR 134
           P+D+V+NS+V+AR
Sbjct: 262 PSDDVVNSVVIAR 273

[Close]

BLAST of HG10006353 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 160.6 bits (405), Expect = 8.7e-40
Identity = 76/133 (57.14%), Postives = 106/133 (79.70%), Query Frame = 0


Query: 1   MALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVF 60
           +A  +LP   FHNFD+DS AN  A+ LV+ D DLS+RM+FHTTD+++ A+  L Q+ VVF
Sbjct: 143 LAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDVLN-ATEGLDQYDVVF 202

Query: 61  LAALGGDEKEEKVKVIEHLRKKLGGGALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFH 120
           LAAL G +KE KVK IEHL K +  GA+LM+RSA+  RAFLYP+V++ DL+GF+++T++H
Sbjct: 203 LAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVDSSDLKGFQLLTIYH 262

Query: 121 PTDEVINSIVLAR 134
           PTD+V+NS+V+AR
Sbjct: 263 PTDDVVNSVVIAR 274

[Close]

BLAST of HG10006353 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 59.7 bits (143), Expect = 2.1e-09
Identity = 27/50 (54.00%), Postives = 40/50 (80.00%), Query Frame = 0


Query: 86  GALLMVRSAYGARAFLYPVVEACDLRGFEIVTVFHP--TDEVINSIVLAR 134
           GALLM+RSA G R+FLY  V+ CDL+GFE++ ++HP  +D  +NS+++AR
Sbjct: 4   GALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVAR 53

The following BLAST results are available for this feature:

BLAST of HG10006353 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891237.1	3.9e-58	88.06	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
XP_008453494.1	1.1e-55	85.07	PREDICTED: nicotianamine synthase 1-like [Cucumis melo]	[more]
KAA0048068.1	1.1e-55	85.07	nicotianamine synthase 1-like [Cucumis melo var. makuwa] >TYJ96468.1 nicotianami...	[more]
XP_004144812.1	6.9e-55	83.58	nicotianamine synthase 1 [Cucumis sativus]	[more]
XP_022965879.1	1.4e-52	82.09	nicotianamine synthase [Cucurbita maxima]	[more]

BLAST of HG10006353 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	2.0e-41	63.91	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	1.7e-40	60.15	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FKT9	2.9e-40	57.89	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]
Q9FF79	1.2e-38	57.14	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9XGI7	2.7e-38	58.65	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]

BLAST of HG10006353 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TYM0	5.1e-56	85.07	Nicotianamine synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BXJ6	5.1e-56	85.07	nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1	[more]
A0A0A0LJ09	3.3e-55	83.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1	[more]
A0A6J1HS54	6.9e-53	82.09	nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1	[more]
A0A6J1FHL8	3.8e-51	80.60	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 S...	[more]

BLAST of HG10006353 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	1.4e-42	63.91	nicotianamine synthase 4	[more]
AT1G09240.1	1.2e-41	60.15	nicotianamine synthase 3	[more]
AT5G56080.1	2.1e-41	57.89	nicotianamine synthase 2	[more]
AT5G04950.1	8.7e-40	57.14	nicotianamine synthase 1	[more]
AT4G26483.1	2.1e-09	54.00	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 2..133 e-value: 1.9E-48 score: 165.2
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 5..133
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..134 score: 23.325285
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..134 e-value: 5.4E-30 score: 106.4
None	No IPR available	PANTHER	PTHR32266:SF16	NICOTIANAMINE SYNTHASE 4-RELATED	coord: 5..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10006353.1	HG10006353.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity