HG10006291 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10006291 vs. NCBI nr
Match: XP_008452708.1 (PREDICTED: uncharacterized protein LOC103493647 isoform X1 [Cucumis melo] >XP_016900949.1 PREDICTED: uncharacterized protein LOC103493647 isoform X1 [Cucumis melo] >TYJ96404.1 Multipolar spindle 1, putative isoform 1 [Cucumis melo var. makuwa])

HSP 1 Score: 625.9 bits (1613), Expect = 2.3e-175
Identity = 334/402 (83.08%), Postives = 351/402 (87.31%), Query Frame = 0

Query: 8   SRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRWKRKAKER 67
           S+IPA SGGIDDQSLKLAIA SLL SKAIQNQ  QH PTVSS PPS S+A RWKRKAKER
Sbjct: 4   SQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSS-PPSHSNALRWKRKAKER 63

Query: 68  KQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFF 127
           KQELIRLRENLKQAEGLC IISSSSLLLSSELI F+ DDLALDASHDD  SQN+TCKCFF
Sbjct: 64  KQELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCFF 123

Query: 128 FDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDFNEDDEVE 187
           FDNLGK NP R E+GTDNRFNDVLRRRFLRQVRYRERRRK+DNAVHRKRLLDFNEDDEVE
Sbjct: 124 FDNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEVE 183

Query: 188 RLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTEGIISSLI 247
           RLQASVDFLVEL DT+S                       DLLPKGR VEL EGIISSLI
Sbjct: 184 RLQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLI 243

Query: 248 MRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLF 307
           MRLIRKM+SPVKDELEDS SD++FY Q LIRELGRETYIGQRAIVSVSQRIS  GESLLF
Sbjct: 244 MRLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLF 303

Query: 308 MDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALEL 367
           MDPFDDNFP+LHE LFIMIQLIEFLISDYL+MWSKD NFDSLLFEE MISI+HAKKALEL
Sbjct: 304 MDPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDRNFDSLLFEEGMISIVHAKKALEL 363

Query: 368 LESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           LESRNGLY+LYMDRVTG+LANQISNIPRLRE+KPDIVDDL+H
Sbjct: 364 LESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of HG10006291 vs. NCBI nr
Match: KAA0056114.1 (Multipolar spindle 1, putative isoform 1 [Cucumis melo var. makuwa])

HSP 1 Score: 623.2 bits (1606), Expect = 1.5e-174
Identity = 332/402 (82.59%), Postives = 351/402 (87.31%), Query Frame = 0

Query: 8   SRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRWKRKAKER 67
           ++IPA SGGIDDQSLKLAIA SLL SKAIQNQ  QH PTVSS PPS S+A RWKRKAKER
Sbjct: 4   TQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSS-PPSHSNALRWKRKAKER 63

Query: 68  KQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFF 127
           KQELIRLRENLKQAEGLC IISSSSLLLSSELI F+ DDLALDASHDD  SQN+TCKCFF
Sbjct: 64  KQELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCFF 123

Query: 128 FDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDFNEDDEVE 187
           FDNLGK NP R E+GTDNRFNDVLRRRFLRQVRYRERRRK+DNAVHRKRL DFNEDDEVE
Sbjct: 124 FDNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLSDFNEDDEVE 183

Query: 188 RLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTEGIISSLI 247
           RLQASVDFLVEL DT+S                       DLLPKGR VEL EGIISSLI
Sbjct: 184 RLQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLI 243

Query: 248 MRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLF 307
           MRLIRKM+SPVKDELEDS SD++FY Q LIRELGRETYIGQRAIVSVSQRIS  GESLLF
Sbjct: 244 MRLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLF 303

Query: 308 MDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALEL 367
           MDPFDDNFP+LHE LFIMIQLIEFLISDYL+MWSKD+NFDSLLFEE MISI+HAKKALEL
Sbjct: 304 MDPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDQNFDSLLFEEGMISIVHAKKALEL 363

Query: 368 LESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           LESRNGLY+LYMDRVTG+LANQISNIPRLRE+KPDIVDDL+H
Sbjct: 364 LESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of HG10006291 vs. NCBI nr
Match: XP_038890473.1 (protein MULTIPOLAR SPINDLE 1 isoform X1 [Benincasa hispida] >XP_038890474.1 protein MULTIPOLAR SPINDLE 1 isoform X1 [Benincasa hispida] >XP_038890475.1 protein MULTIPOLAR SPINDLE 1 isoform X1 [Benincasa hispida] >XP_038890476.1 protein MULTIPOLAR SPINDLE 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 611.7 bits (1576), Expect = 4.4e-171
Identity = 328/409 (80.20%), Postives = 342/409 (83.62%), Query Frame = 0

Query: 1   MSVADGRSRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRW 60
           MSVA+GRS+IPA SGGIDDQSLKLA+AISLLRSKAIQNQQ Q+PPTVSSPP SDSDA RW
Sbjct: 1   MSVANGRSQIPAASGGIDDQSLKLAVAISLLRSKAIQNQQRQNPPTVSSPPSSDSDALRW 60

Query: 61  KRKAKERKQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQN 120
           KRKAKERKQELIRLRE LKQAE                         A+DASHDDL SQN
Sbjct: 61  KRKAKERKQELIRLREVLKQAE-------------------------AVDASHDDLLSQN 120

Query: 121 ATCKCFFFDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDF 180
           ATCKCFFFDN+GK NP R E+GTDNRFNDVLRRRFLRQVRYRER RKADNAVHRKRLLDF
Sbjct: 121 ATCKCFFFDNMGKLNPKRLEDGTDNRFNDVLRRRFLRQVRYRERGRKADNAVHRKRLLDF 180

Query: 181 NEDDEVERLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTE 240
           NEDDEVERLQASVDFLVELCDT+S                       DLLPKGRN+EL E
Sbjct: 181 NEDDEVERLQASVDFLVELCDTYSPVGEANFKNWAHQAVDFILASLKDLLPKGRNMELIE 240

Query: 241 GIISSLIMRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRIST 300
           GIISSLIMRL+RKM SPVKDEL DSVSDIRFYTQQLIRELGRETYIGQRAIV VSQRIS 
Sbjct: 241 GIISSLIMRLVRKMGSPVKDELGDSVSDIRFYTQQLIRELGRETYIGQRAIVYVSQRISA 300

Query: 301 AGESLLFMDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILH 360
           AGESLLFMDPFDDN+P+LHECLFIMIQLIEF+ SDYLQMWSKDENFD LLFEEWMISILH
Sbjct: 301 AGESLLFMDPFDDNYPSLHECLFIMIQLIEFMTSDYLQMWSKDENFDRLLFEEWMISILH 360

Query: 361 AKKALELLESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           AKKALELLESRNGLYVLYMDRVT ELANQISNI RLRELKPDIVDDLI+
Sbjct: 361 AKKALELLESRNGLYVLYMDRVTNELANQISNIRRLRELKPDIVDDLIN 384

BLAST of HG10006291 vs. NCBI nr
Match: XP_038890478.1 (protein MULTIPOLAR SPINDLE 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 609.0 bits (1569), Expect = 2.9e-170
Identity = 327/409 (79.95%), Postives = 340/409 (83.13%), Query Frame = 0

Query: 1   MSVADGRSRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRW 60
           MSVA+GRS+IPA SGGIDDQSLKLA+AISLLRSKAIQNQQ Q+PPTVSSPP SDSDA RW
Sbjct: 1   MSVANGRSQIPAASGGIDDQSLKLAVAISLLRSKAIQNQQRQNPPTVSSPPSSDSDALRW 60

Query: 61  KRKAKERKQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQN 120
           KRKAKERKQELIRLRE LKQAE                           DASHDDL SQN
Sbjct: 61  KRKAKERKQELIRLREVLKQAE---------------------------DASHDDLLSQN 120

Query: 121 ATCKCFFFDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDF 180
           ATCKCFFFDN+GK NP R E+GTDNRFNDVLRRRFLRQVRYRER RKADNAVHRKRLLDF
Sbjct: 121 ATCKCFFFDNMGKLNPKRLEDGTDNRFNDVLRRRFLRQVRYRERGRKADNAVHRKRLLDF 180

Query: 181 NEDDEVERLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTE 240
           NEDDEVERLQASVDFLVELCDT+S                       DLLPKGRN+EL E
Sbjct: 181 NEDDEVERLQASVDFLVELCDTYSPVGEANFKNWAHQAVDFILASLKDLLPKGRNMELIE 240

Query: 241 GIISSLIMRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRIST 300
           GIISSLIMRL+RKM SPVKDEL DSVSDIRFYTQQLIRELGRETYIGQRAIV VSQRIS 
Sbjct: 241 GIISSLIMRLVRKMGSPVKDELGDSVSDIRFYTQQLIRELGRETYIGQRAIVYVSQRISA 300

Query: 301 AGESLLFMDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILH 360
           AGESLLFMDPFDDN+P+LHECLFIMIQLIEF+ SDYLQMWSKDENFD LLFEEWMISILH
Sbjct: 301 AGESLLFMDPFDDNYPSLHECLFIMIQLIEFMTSDYLQMWSKDENFDRLLFEEWMISILH 360

Query: 361 AKKALELLESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           AKKALELLESRNGLYVLYMDRVT ELANQISNI RLRELKPDIVDDLI+
Sbjct: 361 AKKALELLESRNGLYVLYMDRVTNELANQISNIRRLRELKPDIVDDLIN 382

BLAST of HG10006291 vs. NCBI nr
Match: XP_008452717.1 (PREDICTED: uncharacterized protein LOC103493647 isoform X2 [Cucumis melo])

HSP 1 Score: 572.8 bits (1475), Expect = 2.3e-159
Identity = 311/402 (77.36%), Postives = 327/402 (81.34%), Query Frame = 0

Query: 8   SRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRWKRKAKER 67
           S+IPA SGGIDDQSLKLAIA SLL SKAIQNQ  QH PTVSS PPS S+A RWKRKAKER
Sbjct: 4   SQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSS-PPSHSNALRWKRKAKER 63

Query: 68  KQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFF 127
           KQELIRLRENLKQAE                           DASHDD  SQN+TCKCFF
Sbjct: 64  KQELIRLRENLKQAE---------------------------DASHDDFLSQNSTCKCFF 123

Query: 128 FDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDFNEDDEVE 187
           FDNLGK NP R E+GTDNRFNDVLRRRFLRQVRYRERRRK+DNAVHRKRLLDFNEDDEVE
Sbjct: 124 FDNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEVE 183

Query: 188 RLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTEGIISSLI 247
           RLQASVDFLVEL DT+S                       DLLPKGR VEL EGIISSLI
Sbjct: 184 RLQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLI 243

Query: 248 MRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLF 307
           MRLIRKM+SPVKDELEDS SD++FY Q LIRELGRETYIGQRAIVSVSQRIS  GESLLF
Sbjct: 244 MRLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLF 303

Query: 308 MDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALEL 367
           MDPFDDNFP+LHE LFIMIQLIEFLISDYL+MWSKD NFDSLLFEE MISI+HAKKALEL
Sbjct: 304 MDPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDRNFDSLLFEEGMISIVHAKKALEL 363

Query: 368 LESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           LESRNGLY+LYMDRVTG+LANQISNIPRLRE+KPDIVDDL+H
Sbjct: 364 LESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 377

BLAST of HG10006291 vs. ExPASy Swiss-Prot
Match: F4KDF5 (Protein MULTIPOLAR SPINDLE 1 OS=Arabidopsis thaliana OX=3702 GN=MPS1 PE=2 SV=2)

HSP 1 Score: 312.0 bits (798), Expect = 9.5e-84
Identity = 183/390 (46.92%), Postives = 251/390 (64.36%), Query Frame = 0

Query: 19  DQSLKLAIAISLLRSKAIQNQQHQHPPTVS-SPPPSDSDAFRWKRKAKERKQELIRLREN 78
           ++SL+LAIA+SLLRSK    Q HQ   + S     S+SDA RWK+KAKERK+E+IRL+E+
Sbjct: 14  EESLRLAIAVSLLRSKF---QNHQSSSSTSRCYVSSESDALRWKQKAKERKKEIIRLQED 73

Query: 79  LKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFFFDNLGKSNPM 138
           LK AE                            + H DLF  NA+CKC+FFDNLG  +  
Sbjct: 74  LKDAE---------------------------SSFHRDLFPANASCKCYFFDNLGVFSGR 133

Query: 139 RFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDFNEDDEVERLQASVDFLV 198
           R  E +++RFNDVLRRRFLR  R R  RRK   +  R +  + + ++E E L+ S+DFL+
Sbjct: 134 RIGEASESRFNDVLRRRFLRLAR-RRSRRKLTRSSQRLQPSEPDYEEEAEHLRISIDFLL 193

Query: 199 ELCDTFSAD--------------------LLPKGRNVELTEGIISSLIMRLIRKMYSPVK 258
           EL +  S D                    L+  GRN+E  E  IS +I +LI +M +PVK
Sbjct: 194 ELSEADSNDSNFSNWSHQAVDFIFASLKKLISMGRNLESVEESISFMITQLITRMCTPVK 253

Query: 259 -DELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLFMDPFDDNFPNL 318
            +E++   + + FY Q LIR+LG E +IGQRAI ++SQRIS   E+LLFMDPFD++FP +
Sbjct: 254 GNEVKQLETSVGFYVQHLIRKLGSEPFIGQRAIFAISQRISILAENLLFMDPFDESFPEM 313

Query: 319 HECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALELLESRNGLYVLY 378
            EC+FI+IQLIEFLI DYL  W+ +E FD+++FEEW+ S++HA+KA++ LE RNGLY+LY
Sbjct: 314 DECMFILIQLIEFLICDYLLPWA-NEAFDNVMFEEWIASVVHARKAVKALEERNGLYLLY 371

Query: 379 MDRVTGELANQISNIPRLRELKPDIVDDLI 387
           MDRVTGELA ++  I   RE++P I+D ++
Sbjct: 374 MDRVTGELAKRVGQITSFREVEPAILDKIL 371

BLAST of HG10006291 vs. ExPASy TrEMBL
Match: A0A1S3BUI0 (uncharacterized protein LOC103493647 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493647 PE=4 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.1e-175
Identity = 334/402 (83.08%), Postives = 351/402 (87.31%), Query Frame = 0

Query: 8   SRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRWKRKAKER 67
           S+IPA SGGIDDQSLKLAIA SLL SKAIQNQ  QH PTVSS PPS S+A RWKRKAKER
Sbjct: 4   SQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSS-PPSHSNALRWKRKAKER 63

Query: 68  KQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFF 127
           KQELIRLRENLKQAEGLC IISSSSLLLSSELI F+ DDLALDASHDD  SQN+TCKCFF
Sbjct: 64  KQELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCFF 123

Query: 128 FDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDFNEDDEVE 187
           FDNLGK NP R E+GTDNRFNDVLRRRFLRQVRYRERRRK+DNAVHRKRLLDFNEDDEVE
Sbjct: 124 FDNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEVE 183

Query: 188 RLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTEGIISSLI 247
           RLQASVDFLVEL DT+S                       DLLPKGR VEL EGIISSLI
Sbjct: 184 RLQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLI 243

Query: 248 MRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLF 307
           MRLIRKM+SPVKDELEDS SD++FY Q LIRELGRETYIGQRAIVSVSQRIS  GESLLF
Sbjct: 244 MRLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLF 303

Query: 308 MDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALEL 367
           MDPFDDNFP+LHE LFIMIQLIEFLISDYL+MWSKD NFDSLLFEE MISI+HAKKALEL
Sbjct: 304 MDPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDRNFDSLLFEEGMISIVHAKKALEL 363

Query: 368 LESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           LESRNGLY+LYMDRVTG+LANQISNIPRLRE+KPDIVDDL+H
Sbjct: 364 LESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of HG10006291 vs. ExPASy TrEMBL
Match: A0A5D3BBS1 (Multipolar spindle 1, putative isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00310 PE=4 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.1e-175
Identity = 334/402 (83.08%), Postives = 351/402 (87.31%), Query Frame = 0

Query: 8   SRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRWKRKAKER 67
           S+IPA SGGIDDQSLKLAIA SLL SKAIQNQ  QH PTVSS PPS S+A RWKRKAKER
Sbjct: 4   SQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSS-PPSHSNALRWKRKAKER 63

Query: 68  KQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFF 127
           KQELIRLRENLKQAEGLC IISSSSLLLSSELI F+ DDLALDASHDD  SQN+TCKCFF
Sbjct: 64  KQELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCFF 123

Query: 128 FDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDFNEDDEVE 187
           FDNLGK NP R E+GTDNRFNDVLRRRFLRQVRYRERRRK+DNAVHRKRLLDFNEDDEVE
Sbjct: 124 FDNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEVE 183

Query: 188 RLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTEGIISSLI 247
           RLQASVDFLVEL DT+S                       DLLPKGR VEL EGIISSLI
Sbjct: 184 RLQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLI 243

Query: 248 MRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLF 307
           MRLIRKM+SPVKDELEDS SD++FY Q LIRELGRETYIGQRAIVSVSQRIS  GESLLF
Sbjct: 244 MRLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLF 303

Query: 308 MDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALEL 367
           MDPFDDNFP+LHE LFIMIQLIEFLISDYL+MWSKD NFDSLLFEE MISI+HAKKALEL
Sbjct: 304 MDPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDRNFDSLLFEEGMISIVHAKKALEL 363

Query: 368 LESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           LESRNGLY+LYMDRVTG+LANQISNIPRLRE+KPDIVDDL+H
Sbjct: 364 LESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of HG10006291 vs. ExPASy TrEMBL
Match: A0A5A7ULP1 (Multipolar spindle 1, putative isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold323G00780 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 7.1e-175
Identity = 332/402 (82.59%), Postives = 351/402 (87.31%), Query Frame = 0

Query: 8   SRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRWKRKAKER 67
           ++IPA SGGIDDQSLKLAIA SLL SKAIQNQ  QH PTVSS PPS S+A RWKRKAKER
Sbjct: 4   TQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSS-PPSHSNALRWKRKAKER 63

Query: 68  KQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFF 127
           KQELIRLRENLKQAEGLC IISSSSLLLSSELI F+ DDLALDASHDD  SQN+TCKCFF
Sbjct: 64  KQELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCFF 123

Query: 128 FDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDFNEDDEVE 187
           FDNLGK NP R E+GTDNRFNDVLRRRFLRQVRYRERRRK+DNAVHRKRL DFNEDDEVE
Sbjct: 124 FDNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLSDFNEDDEVE 183

Query: 188 RLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTEGIISSLI 247
           RLQASVDFLVEL DT+S                       DLLPKGR VEL EGIISSLI
Sbjct: 184 RLQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLI 243

Query: 248 MRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLF 307
           MRLIRKM+SPVKDELEDS SD++FY Q LIRELGRETYIGQRAIVSVSQRIS  GESLLF
Sbjct: 244 MRLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLF 303

Query: 308 MDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALEL 367
           MDPFDDNFP+LHE LFIMIQLIEFLISDYL+MWSKD+NFDSLLFEE MISI+HAKKALEL
Sbjct: 304 MDPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDQNFDSLLFEEGMISIVHAKKALEL 363

Query: 368 LESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           LESRNGLY+LYMDRVTG+LANQISNIPRLRE+KPDIVDDL+H
Sbjct: 364 LESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of HG10006291 vs. ExPASy TrEMBL
Match: A0A0A0LK37 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G036060 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.0e-173
Identity = 329/402 (81.84%), Postives = 350/402 (87.06%), Query Frame = 0

Query: 8   SRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRWKRKAKER 67
           S++PA SGG+DD SLKLAIA S L S+A QNQ  QHPPTVSS   SDS+A RWKRKAKER
Sbjct: 4   SQVPAASGGLDDLSLKLAIASSFLPSEAYQNQHQQHPPTVSS-LSSDSNALRWKRKAKER 63

Query: 68  KQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFF 127
           KQELIRLRENLKQAEGLC+IISSSSLLLSSELIRFV DDL LDASHDDL SQN++CKCFF
Sbjct: 64  KQELIRLRENLKQAEGLCIIISSSSLLLSSELIRFVSDDLTLDASHDDLLSQNSSCKCFF 123

Query: 128 FDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDFNEDDEVE 187
           FDNLGK NP R ++GTDNRFNDVLRRRFLRQVRYRERRRK+D+AVHRKR+LDFNEDDEVE
Sbjct: 124 FDNLGKLNPTRLQDGTDNRFNDVLRRRFLRQVRYRERRRKSDDAVHRKRVLDFNEDDEVE 183

Query: 188 RLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTEGIISSLI 247
           RLQASVDFLVEL DT+S                       DLLPKGR VEL EGIISSLI
Sbjct: 184 RLQASVDFLVELYDTYSPVGEANFKNWAHQAVDFILASLKDLLPKGRYVELIEGIISSLI 243

Query: 248 MRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLF 307
           MRLIRKM SPVKDELEDS SD++FYTQ LIRELGRETYIGQRAIVSVSQRIS AGESLLF
Sbjct: 244 MRLIRKMRSPVKDELEDSASDVQFYTQHLIRELGRETYIGQRAIVSVSQRISAAGESLLF 303

Query: 308 MDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALEL 367
           MDPFDDNFP+LHE LF+MIQLIE LISDYLQMWSKDENFDSLLFEE MISILHAKKALEL
Sbjct: 304 MDPFDDNFPSLHEYLFLMIQLIELLISDYLQMWSKDENFDSLLFEEGMISILHAKKALEL 363

Query: 368 LESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           LESRNGLY+LYMDRVTG+LANQI NIPRLRE+KPDIVDDLIH
Sbjct: 364 LESRNGLYILYMDRVTGDLANQICNIPRLREIKPDIVDDLIH 404

BLAST of HG10006291 vs. ExPASy TrEMBL
Match: A0A1S3BUG1 (uncharacterized protein LOC103493647 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493647 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 1.1e-159
Identity = 311/402 (77.36%), Postives = 327/402 (81.34%), Query Frame = 0

Query: 8   SRIPAESGGIDDQSLKLAIAISLLRSKAIQNQQHQHPPTVSSPPPSDSDAFRWKRKAKER 67
           S+IPA SGGIDDQSLKLAIA SLL SKAIQNQ  QH PTVSS PPS S+A RWKRKAKER
Sbjct: 4   SQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSS-PPSHSNALRWKRKAKER 63

Query: 68  KQELIRLRENLKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFF 127
           KQELIRLRENLKQAE                           DASHDD  SQN+TCKCFF
Sbjct: 64  KQELIRLRENLKQAE---------------------------DASHDDFLSQNSTCKCFF 123

Query: 128 FDNLGKSNPMRFEEGTDNRFNDVLRRRFLRQVRYRERRRKADNAVHRKRLLDFNEDDEVE 187
           FDNLGK NP R E+GTDNRFNDVLRRRFLRQVRYRERRRK+DNAVHRKRLLDFNEDDEVE
Sbjct: 124 FDNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEVE 183

Query: 188 RLQASVDFLVELCDTFS----------------------ADLLPKGRNVELTEGIISSLI 247
           RLQASVDFLVEL DT+S                       DLLPKGR VEL EGIISSLI
Sbjct: 184 RLQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLI 243

Query: 248 MRLIRKMYSPVKDELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLF 307
           MRLIRKM+SPVKDELEDS SD++FY Q LIRELGRETYIGQRAIVSVSQRIS  GESLLF
Sbjct: 244 MRLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLF 303

Query: 308 MDPFDDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALEL 367
           MDPFDDNFP+LHE LFIMIQLIEFLISDYL+MWSKD NFDSLLFEE MISI+HAKKALEL
Sbjct: 304 MDPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDRNFDSLLFEEGMISIVHAKKALEL 363

Query: 368 LESRNGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLIH 388
           LESRNGLY+LYMDRVTG+LANQISNIPRLRE+KPDIVDDL+H
Sbjct: 364 LESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 377

BLAST of HG10006291 vs. TAIR 10
Match: AT5G57880.1 (multipolar spindle 1 )

HSP 1 Score: 307.8 bits (787), Expect = 1.3e-83
Identity = 182/397 (45.84%), Postives = 251/397 (63.22%), Query Frame = 0

Query: 19  DQSLKLAIAISLLRSKAIQNQQHQHPPTVS-SPPPSDSDAFRWKRKAKERKQELIRLREN 78
           ++SL+LAIA+SLLRSK    Q HQ   + S     S+SDA RWK+KAKERK+E+IRL+E+
Sbjct: 14  EESLRLAIAVSLLRSKF---QNHQSSSSTSRCYVSSESDALRWKQKAKERKKEIIRLQED 73

Query: 79  LKQAEGLCVIISSSSLLLSSELIRFVCDDLALDASHDDLFSQNATCKCFFFDNLGKSNPM 138
           LK AE                            + H DLF  NA+CKC+FFDNLG  +  
Sbjct: 74  LKDAE---------------------------SSFHRDLFPANASCKCYFFDNLGVFSGR 133

Query: 139 RFEEGTDNRFNDVLRRRFLR-------QVRYRERRRKADNAVHRKRLLDFNEDDEVERLQ 198
           R  E +++RFNDVLRRRFLR        +  R  RRK   +  R +  + + ++E E L+
Sbjct: 134 RIGEASESRFNDVLRRRFLRLACVVILSLARRRSRRKLTRSSQRLQPSEPDYEEEAEHLR 193

Query: 199 ASVDFLVELCDTFSAD--------------------LLPKGRNVELTEGIISSLIMRLIR 258
            S+DFL+EL +  S D                    L+  GRN+E  E  IS +I +LI 
Sbjct: 194 ISIDFLLELSEADSNDSNFSNWSHQAVDFIFASLKKLISMGRNLESVEESISFMITQLIT 253

Query: 259 KMYSPVK-DELEDSVSDIRFYTQQLIRELGRETYIGQRAIVSVSQRISTAGESLLFMDPF 318
           +M +PVK +E++   + + FY Q LIR+LG E +IGQRAI ++SQRIS   E+LLFMDPF
Sbjct: 254 RMCTPVKGNEVKQLETSVGFYVQHLIRKLGSEPFIGQRAIFAISQRISILAENLLFMDPF 313

Query: 319 DDNFPNLHECLFIMIQLIEFLISDYLQMWSKDENFDSLLFEEWMISILHAKKALELLESR 378
           D++FP + EC+FI+IQLIEFLI DYL  W+ +E FD+++FEEW+ S++HA+KA++ LE R
Sbjct: 314 DESFPEMDECMFILIQLIEFLICDYLLPWA-NEAFDNVMFEEWIASVVHARKAVKALEER 373

Query: 379 NGLYVLYMDRVTGELANQISNIPRLRELKPDIVDDLI 387
           NGLY+LYMDRVTGELA ++  I   RE++P I+D ++
Sbjct: 374 NGLYLLYMDRVTGELAKRVGQITSFREVEPAILDKIL 379