HG10006253 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10006253
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description26S proteasome regulatory subunit 4 homolog
LocationChr07: 16465689 .. 16468226 (+)
RNA-Seq ExpressionHG10006253
SyntenyHG10006253
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCAAGGAACTCCCGGCGGACTCAACCGGCAGGGCGGTCTTCCCGGCGACCGCAAGCAAGATGATCCCAACAAGAAGGACAAGAAATTCGAGCCTGCGGCGCCTCCGTCGCGTGTCGGCCGCAAACAGCGCAAGCAGAAGGGTCCCGACGCCGCCGCCCGCCTCCCGACGGTCACTCCCCACACCAAGTGCAAGCTCCGCCTTCTGAAGCTCGAGCGAGTCAAGGACTATTTGCTCATGGAGGAGGAGTTTGTTACCAATCAGGAGCGGTTGAAGCCCCAGGAGGAGAAGAATGAAGAAGATAGATCTAAGGTTGACGACCTTCGTGGGTCGCCGATGAGCGTTGGGAATCTGGAGGAGCTGATTGATGAGAATCATGCGATTGTGTCGTCGTCTGTCGGTCCGGAGTACTATGTTGGGATCTTGTCGTTTGTCGATAAAGACCAGCTGGAGCCTGGATGTGCTATTCTTATGCACAACAAGGTAGGTTTTGTTATGGTTGTTTATCTGGCATTGTTTCGTCGCTGTAATTGGAAATTGCCGAAGAATTGGATAAACCTGCATATTTATTCGATGGATTGTTGCAATTTTTCTGTTTACTTTTCGCTACTCTTCGATTCTAGGTTATTAGGCTGTTGCATTTATCTCGTTCATGTGGATTTAATATGTATACATATCAACTTCTCTAGGAGGACAATCTCAGGTTAGGGTTGGTTGGCTTTGGGAAATGTTGAACAAAAAATGAGCCCTAGATTATGTTTTATGATGACATTGAGTTATGGTGAAGTGCAATATTGTATCCTGTTAAAAATTACTTTTCTATGTTGTGAAATGTGGATGGTTTTTTTGTGTTTTACTCAGGTCCTTTCTGTTGTTGGGCTTCTTCAAGATGAGGTCGACCCCATGGTATCTGTTATGAAGGTTGAGAAGGCTCCATTGGAATCCTATGCTGATATTGGTGGTTTAGATGCTCAGATCCAGGAGATTAAAGAAGCTGTAGAACTTCCTCTTACTCATCCTGAATTATATGAAGACATTGGTATTAAACCCCCCAAAGGAGTTATACTTTATGGGGAGCCAGGGACAGGGAAAACATTGCTCGCAAAGGTATCTATTAACTTAGCCACACACACACACAAAATTGTGTTACTCTGGAATTAGGACGATTATTGATTTAGCAACTTTGTAATATTTATTCGAATTCTCATGCTTGTGAAATGGTTGTTAGAATTTTTATATTTGCTCGGCGTTGATTAATGAAAACATTGCTCGCAAAGGTATCCATTAACTTAGCCACACACACACGCAAAATTGTGTTACTCTGGAATTAGGACGATTACTGATTTAGCAACTGTGTAATATTTATTTCAACTCTCATGCTAGTGAAATGGTTGTTAGAAGTTTTATATTTGTTCGGTGTCTATTAATGAGTGGGTTTTTTATTTTTGAATTTGCAGTGAAGTATTTGCAACATAGGTTGTTCTTGACTTAAACTGTGTTATTAACTTGATTGTGTTCAGGCCGTGGCAAACTCCACTTCAGCCACTTTCTTGCGTGTAGTTGGAAGTGAATTGATCCAAAAGTACTTGGGAGATGGACCTAAGTTGGTCAGGGAGCTCTTTAGAGTTGCAGATGATCTTTCCCCGTCAATTGTATTCATTGATGAAATTGACGCAGTTGGTACAAAGAGGTGTTCATTCTAGTGAAAATTATTGTGACATGTTTTCTATCTTGTGTGTAAGCAGCATTCCTTATTGCGTTGCACGTATTACATGTTGGCCAGGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAAAGGACCATGCTTGAATTACTGAACCAATTGGATGGGTTTGACTCAAGAGGAGATGTTAAAGTAATTCTTGCTACAAATAGAATTGAAAGCCTTGATCCTGCCCTTTTACGACCTGGAAGAATAGATAGAAAAATTGAATTTCCCCTTCCAGATATAAAAACAAGACGACGCATTTTTCAGGTGCTTCAAATACCTTTTTGCATGGAGAAAATCGATTGCAATTGGCTTTTTTTTTTAATCATATAATTTTATTAACGCTGCTGTTCATAAAATATAGATTCATACATCAAGGATGACCCTAGCTGACGATGTGAACTTAGAAGAGTTTGTAATGACCAAAGACGAATTTTCTGGGGCTGATATCAAAGCAATTTGCACAGAAGCTGGCTTGCTTGCTCTAAGAGAACGTAGAATGAAGGTAAATATTATTTACATAGCAACTTTTCATAAACTTGTACATGAAATTTGTTCTCTACCTATAAATGTAGTGCATTTTAGCTGGTGATAAAGTTGATAATATCTGAAGATTGGTAGTTAAACAGTGAGGGTTTCTGTTTTTATACATCTTAAAACGCTGGTATAATATGTTTTCGTGCTCGATGTTTCCTAACTAACTTGCTTTGTATTTCAGGTGACTCATGCAGACTTTAAGAAAGCAAAAGAGAAGGTTATGTTCAAAAAGAAAGAGGGTGTTCCAGAAGGACTTTACATGTAA

mRNA sequence

ATGGGTCAAGGAACTCCCGGCGGACTCAACCGGCAGGGCGGTCTTCCCGGCGACCGCAAGCAAGATGATCCCAACAAGAAGGACAAGAAATTCGAGCCTGCGGCGCCTCCGTCGCGTGTCGGCCGCAAACAGCGCAAGCAGAAGGGTCCCGACGCCGCCGCCCGCCTCCCGACGGTCACTCCCCACACCAAGTGCAAGCTCCGCCTTCTGAAGCTCGAGCGAGTCAAGGACTATTTGCTCATGGAGGAGGAGTTTGTTACCAATCAGGAGCGGTTGAAGCCCCAGGAGGAGAAGAATGAAGAAGATAGATCTAAGGTTGACGACCTTCGTGGGTCGCCGATGAGCGTTGGGAATCTGGAGGAGCTGATTGATGAGAATCATGCGATTGTGTCGTCGTCTGTCGGTCCGGAGTACTATGTTGGGATCTTGTCGTTTGTCGATAAAGACCAGCTGGAGCCTGGATGTGCTATTCTTATGCACAACAAGGTCCTTTCTGTTGTTGGGCTTCTTCAAGATGAGGTCGACCCCATGGTATCTGTTATGAAGGTTGAGAAGGCTCCATTGGAATCCTATGCTGATATTGGTGGTTTAGATGCTCAGATCCAGGAGATTAAAGAAGCTGTAGAACTTCCTCTTACTCATCCTGAATTATATGAAGACATTGGTATTAAACCCCCCAAAGGAGTTATACTTTATGGGGAGCCAGGGACAGGGAAAACATTGCTCGCAAAGGCCGTGGCAAACTCCACTTCAGCCACTTTCTTGCGTGTAGTTGGAAGTGAATTGATCCAAAAGTACTTGGGAGATGGACCTAAGTTGGTCAGGGAGCTCTTTAGAGTTGCAGATGATCTTTCCCCGTCAATTGTATTCATTGATGAAATTGACGCAGTTGGTACAAAGAGGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAAAGGACCATGCTTGAATTACTGAACCAATTGGATGGGTTTGACTCAAGAGGAGATGTTAAAGTAATTCTTGCTACAAATAGAATTGAAAGCCTTGATCCTGCCCTTTTACGACCTGGAAGAATAGATAGAAAAATTGAATTTCCCCTTCCAGATATAAAAACAAGACGACGCATTTTTCAGATTCATACATCAAGGATGACCCTAGCTGACGATGTGAACTTAGAAGAGTTTGTAATGACCAAAGACGAATTTTCTGGGGCTGATATCAAAGCAATTTGCACAGAAGCTGGCTTGCTTGCTCTAAGAGAACGTAGAATGAAGGTGACTCATGCAGACTTTAAGAAAGCAAAAGAGAAGGTTATGTTCAAAAAGAAAGAGGGTGTTCCAGAAGGACTTTACATGTAA

Coding sequence (CDS)

ATGGGTCAAGGAACTCCCGGCGGACTCAACCGGCAGGGCGGTCTTCCCGGCGACCGCAAGCAAGATGATCCCAACAAGAAGGACAAGAAATTCGAGCCTGCGGCGCCTCCGTCGCGTGTCGGCCGCAAACAGCGCAAGCAGAAGGGTCCCGACGCCGCCGCCCGCCTCCCGACGGTCACTCCCCACACCAAGTGCAAGCTCCGCCTTCTGAAGCTCGAGCGAGTCAAGGACTATTTGCTCATGGAGGAGGAGTTTGTTACCAATCAGGAGCGGTTGAAGCCCCAGGAGGAGAAGAATGAAGAAGATAGATCTAAGGTTGACGACCTTCGTGGGTCGCCGATGAGCGTTGGGAATCTGGAGGAGCTGATTGATGAGAATCATGCGATTGTGTCGTCGTCTGTCGGTCCGGAGTACTATGTTGGGATCTTGTCGTTTGTCGATAAAGACCAGCTGGAGCCTGGATGTGCTATTCTTATGCACAACAAGGTCCTTTCTGTTGTTGGGCTTCTTCAAGATGAGGTCGACCCCATGGTATCTGTTATGAAGGTTGAGAAGGCTCCATTGGAATCCTATGCTGATATTGGTGGTTTAGATGCTCAGATCCAGGAGATTAAAGAAGCTGTAGAACTTCCTCTTACTCATCCTGAATTATATGAAGACATTGGTATTAAACCCCCCAAAGGAGTTATACTTTATGGGGAGCCAGGGACAGGGAAAACATTGCTCGCAAAGGCCGTGGCAAACTCCACTTCAGCCACTTTCTTGCGTGTAGTTGGAAGTGAATTGATCCAAAAGTACTTGGGAGATGGACCTAAGTTGGTCAGGGAGCTCTTTAGAGTTGCAGATGATCTTTCCCCGTCAATTGTATTCATTGATGAAATTGACGCAGTTGGTACAAAGAGGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAAAGGACCATGCTTGAATTACTGAACCAATTGGATGGGTTTGACTCAAGAGGAGATGTTAAAGTAATTCTTGCTACAAATAGAATTGAAAGCCTTGATCCTGCCCTTTTACGACCTGGAAGAATAGATAGAAAAATTGAATTTCCCCTTCCAGATATAAAAACAAGACGACGCATTTTTCAGATTCATACATCAAGGATGACCCTAGCTGACGATGTGAACTTAGAAGAGTTTGTAATGACCAAAGACGAATTTTCTGGGGCTGATATCAAAGCAATTTGCACAGAAGCTGGCTTGCTTGCTCTAAGAGAACGTAGAATGAAGGTGACTCATGCAGACTTTAAGAAAGCAAAAGAGAAGGTTATGTTCAAAAAGAAAGAGGGTGTTCCAGAAGGACTTTACATGTAA

Protein sequence

MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVTPHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHADFKKAKEKVMFKKKEGVPEGLYM
Homology
BLAST of HG10006253 vs. NCBI nr
Match: XP_004149071.1 (26S proteasome regulatory subunit 4 homolog B [Cucumis sativus] >XP_008452203.1 PREDICTED: LOW QUALITY PROTEIN: 26S proteasome regulatory subunit 4 homolog B-like [Cucumis melo] >XP_038890843.1 26S proteasome regulatory subunit 4 homolog B [Benincasa hispida] >KAA0067260.1 26S proteasome regulatory subunit 4-like protein A [Cucumis melo var. makuwa] >KGN61098.1 hypothetical protein Csa_021333 [Cucumis sativus] >TYK10869.1 26S proteasome regulatory subunit 4-like protein A [Cucumis melo var. makuwa])

HSP 1 Score: 868.6 bits (2243), Expect = 2.3e-248
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT
Sbjct: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 447

BLAST of HG10006253 vs. NCBI nr
Match: XP_022153378.1 (26S proteasome regulatory subunit 4 homolog B-like [Momordica charantia])

HSP 1 Score: 866.7 bits (2238), Expect = 8.8e-248
Identity = 446/447 (99.78%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT
Sbjct: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKLERVKDYLLMEEEFV NQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 61  PHTKCKLRLLKLERVKDYLLMEEEFVANQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 447

BLAST of HG10006253 vs. NCBI nr
Match: KAG7029796.1 (26S proteasome regulatory subunit 4-like B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 860.5 bits (2222), Expect = 6.3e-246
Identity = 441/447 (98.66%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAA+RLPTVT
Sbjct: 32  MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAASRLPTVT 91

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKL+RVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 92  PHTKCKLRLLKLDRVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 151

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 152 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 211

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MK+EKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 212 MKIEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 271

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADD SPSIVFIDEIDAVGTK
Sbjct: 272 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDHSPSIVFIDEIDAVGTK 331

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 332 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 391

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLA+DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 392 PLPDIKTRRRIFQIHTSRMTLAEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 451

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAK+KVMFKKKEGVPEGLYM
Sbjct: 452 VTHADFKKAKDKVMFKKKEGVPEGLYM 478

BLAST of HG10006253 vs. NCBI nr
Match: XP_022929739.1 (26S proteasome regulatory subunit 4 homolog B-like [Cucurbita moschata] >KAG6598855.1 26S proteasome regulatory subunit 4-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 860.5 bits (2222), Expect = 6.3e-246
Identity = 441/447 (98.66%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAA+RLPTVT
Sbjct: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAASRLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKL+RVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 61  PHTKCKLRLLKLDRVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MK+EKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKIEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADD SPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDHSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLA+DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSRMTLAEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAK+KVMFKKKEGVPEGLYM
Sbjct: 421 VTHADFKKAKDKVMFKKKEGVPEGLYM 447

BLAST of HG10006253 vs. NCBI nr
Match: XP_022997411.1 (26S proteasome regulatory subunit 4 homolog B-like [Cucurbita maxima] >XP_023546172.1 26S proteasome regulatory subunit 4 homolog B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 859.0 bits (2218), Expect = 1.8e-245
Identity = 440/447 (98.43%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLN+QGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAA+RLPTVT
Sbjct: 1   MGQGTPGGLNQQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAASRLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKL+RVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 61  PHTKCKLRLLKLDRVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MK+EKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKIEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADD SPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDHSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLA+DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSRMTLAEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAK+KVMFKKKEGVPEGLYM
Sbjct: 421 VTHADFKKAKDKVMFKKKEGVPEGLYM 447

BLAST of HG10006253 vs. ExPASy Swiss-Prot
Match: Q9SZD4 (26S proteasome regulatory subunit 4 homolog A OS=Arabidopsis thaliana OX=3702 GN=RPT2A PE=1 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 3.0e-235
Identity = 422/447 (94.41%), Postives = 434/447 (97.09%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQG  GGLNRQ    GDRK D  +KK+KKFEPAAPP+RVGRKQRKQKGP+AAARLPTVT
Sbjct: 1   MGQGPSGGLNRQ----GDRKPDGGDKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           P TKCKLRLLKLER+KDYLLMEEEFV NQERLKPQEEK EEDRSKVDDLRG+PMSVGNLE
Sbjct: 61  PSTKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGL+AQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTS+MTL++DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSKMTLSEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTH DFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHPDFKKAKEKVMFKKKEGVPEGLYM 443

BLAST of HG10006253 vs. ExPASy Swiss-Prot
Match: Q9SL67 (26S proteasome regulatory subunit 4 homolog B OS=Arabidopsis thaliana OX=3702 GN=RPT2B PE=1 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 8.9e-235
Identity = 422/447 (94.41%), Postives = 433/447 (96.87%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQG  GGLNRQ    GDRK D   KK+KKFEPAAPP+RVGRKQRKQKGP+AAARLPTVT
Sbjct: 1   MGQGPSGGLNRQ----GDRKPDGGEKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           P TKCKLRLLKLER+KDYLLMEEEFV NQERLKPQEEK EEDRSKVDDLRG+PMSVGNLE
Sbjct: 61  PSTKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGL+AQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDA+SGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDANSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTS+MTLA+DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSKMTLAEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTH DFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHVDFKKAKEKVMFKKKEGVPEGLYM 443

BLAST of HG10006253 vs. ExPASy Swiss-Prot
Match: P46466 (26S proteasome regulatory subunit 4 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=TBP2 PE=2 SV=2)

HSP 1 Score: 805.4 bits (2079), Expect = 3.2e-232
Identity = 415/448 (92.63%), Postives = 433/448 (96.65%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQ-DDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTV 60
           MGQGTPGG+ +QGG PGDRK   D +KKD+KFEP A PSRVGRKQRKQKGP+AAARLP V
Sbjct: 1   MGQGTPGGMGKQGGAPGDRKPGGDGDKKDRKFEPPAAPSRVGRKQRKQKGPEAAARLPNV 60

Query: 61  TPHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNL 120
            P +KC+LRLLKLERVKDYLLMEEEFV  QERL+P E+K EEDRSKVDDLRG+PMSVG+L
Sbjct: 61  APLSKCRLRLLKLERVKDYLLMEEEFVAAQERLRPTEDKTEEDRSKVDDLRGTPMSVGSL 120

Query: 121 EELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVS 180
           EE+IDE+HAIVSSSVGPEYYVGILSFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVS
Sbjct: 121 EEIIDESHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVS 180

Query: 181 VMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGK 240
           VMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGI+PPKGVILYGEPGTGK
Sbjct: 181 VMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGK 240

Query: 241 TLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGT 300
           TLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVAD+LSPSIVFIDEIDAVGT
Sbjct: 241 TLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADELSPSIVFIDEIDAVGT 300

Query: 301 KRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIE 360
           KRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIE
Sbjct: 301 KRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIE 360

Query: 361 FPLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRM 420
           FPLPDIKTRRRIFQIHTS+MTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRM
Sbjct: 361 FPLPDIKTRRRIFQIHTSKMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRM 420

Query: 421 KVTHADFKKAKEKVMFKKKEGVPEGLYM 448
           KVTHADFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 KVTHADFKKAKEKVMFKKKEGVPEGLYM 448

BLAST of HG10006253 vs. ExPASy Swiss-Prot
Match: P48601 (26S proteasome regulatory subunit 4 OS=Drosophila melanogaster OX=7227 GN=Rpt2 PE=1 SV=2)

HSP 1 Score: 690.3 bits (1780), Expect = 1.5e-197
Identity = 347/436 (79.59%), Postives = 402/436 (92.20%), Query Frame = 0

Query: 12  QGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVTPHTKCKLRLLK 71
           QGG     K+DD +KK KK+EP   P+RVG+K+R+ KGPDAA +LP VTPHT+C+L+LLK
Sbjct: 8   QGG--AGEKKDDKDKK-KKYEPPI-PTRVGKKKRRAKGPDAAMKLPQVTPHTRCRLKLLK 67

Query: 72  LERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLEELIDENHAIVS 131
           LER+KDYL+ME+EF+ NQERLKPQ+EKNEE+RSKVDDLRG+PMSVGNLEE+ID+NHAIVS
Sbjct: 68  LERIKDYLMMEDEFIRNQERLKPQDEKNEEERSKVDDLRGTPMSVGNLEEIIDDNHAIVS 127

Query: 132 SSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMKVEKAPLESY 191
           +SVG E+YV ILSFVDKDQLEPGC++L+++KV +VVG+L D+ DPMV+VMK+EKAP E+Y
Sbjct: 128 TSVGSEHYVSILSFVDKDQLEPGCSVLLNHKVHAVVGVLSDDTDPMVTVMKLEKAPQETY 187

Query: 192 ADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTS 251
           ADIGGLD QIQEIKE+VELPLTHPE YE++GIKPPKGVILYG PGTGKTLLAKAVAN TS
Sbjct: 188 ADIGGLDTQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTS 247

Query: 252 ATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGERE 311
           ATFLRVVGSELIQKYLGDGPKLVRELFRVA++ +PSIVFIDEIDAVGTKRYD++SGGERE
Sbjct: 248 ATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAVGTKRYDSNSGGERE 307

Query: 312 IQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRI 371
           IQRTMLELLNQLDGFDSRGDVKVI+ATNRIE+LDPAL+RPGRIDRKIEFPLPD KT+RRI
Sbjct: 308 IQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKRRI 367

Query: 372 FQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHADFKKAKE 431
           F IHTSRMTLA+DVNL E +M KD+ SGADIKAICTEAGL+ALRERRMKVT+ DFKK+KE
Sbjct: 368 FTIHTSRMTLAEDVNLSELIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKE 427

Query: 432 KVMFKKKEGVPEGLYM 448
            V+++KKEG PEGLY+
Sbjct: 428 SVLYRKKEGTPEGLYL 439

BLAST of HG10006253 vs. ExPASy Swiss-Prot
Match: P62191 (26S proteasome regulatory subunit 4 OS=Homo sapiens OX=9606 GN=PSMC1 PE=1 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 1.1e-195
Identity = 345/447 (77.18%), Postives = 404/447 (90.38%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQ   GG       PG  K+DD +KK KK+EP   P+RVG+K++K KGPDAA++LP VT
Sbjct: 1   MGQSQSGGHG-----PGGGKKDDKDKK-KKYEPPV-PTRVGKKKKKTKGPDAASKLPLVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHT+C+L+LLKLER+KDYLLMEEEF+ NQE++KP EEK EE+RSKVDDLRG+PMSVG LE
Sbjct: 61  PHTQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           E+ID+NHAIVS+SVG E+YV ILSFVDKD LEPGC++L+++KV +V+G+L D+ DP+V+V
Sbjct: 121 EIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAP E+YADIGGLD QIQEIKE+VELPLTHPE YE++GIKPPKGVILYG PGTGKT
Sbjct: 181 MKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVAN TSATFLRVVGSELIQKYLGDGPKLVRELFRVA++ +PSIVFIDEIDA+GTK
Sbjct: 241 LLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYD++SGGEREIQRTMLELLNQLDGFDSRGDVKVI+ATNRIE+LDPAL+RPGRIDRKIEF
Sbjct: 301 RYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPD KT++RIFQIHTSRMTLADDV L++ +M KD+ SGADIKAICTEAGL+ALRERRMK
Sbjct: 361 PLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VT+ DFKK+KE V++KK+EG PEGLY+
Sbjct: 421 VTNEDFKKSKENVLYKKQEGTPEGLYL 440

BLAST of HG10006253 vs. ExPASy TrEMBL
Match: A0A5D3CKV4 (26S proteasome regulatory subunit 4-like protein A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00410 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.1e-248
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT
Sbjct: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 447

BLAST of HG10006253 vs. ExPASy TrEMBL
Match: A0A0A0LH67 (AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G049870 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.1e-248
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT
Sbjct: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 447

BLAST of HG10006253 vs. ExPASy TrEMBL
Match: A0A1S3BUF2 (LOW QUALITY PROTEIN: 26S proteasome regulatory subunit 4 homolog B-like OS=Cucumis melo OX=3656 GN=LOC103493290 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.1e-248
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT
Sbjct: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 447

BLAST of HG10006253 vs. ExPASy TrEMBL
Match: A0A6J1DHA6 (26S proteasome regulatory subunit 4 homolog B-like OS=Momordica charantia OX=3673 GN=LOC111020888 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.3e-248
Identity = 446/447 (99.78%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT
Sbjct: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKLERVKDYLLMEEEFV NQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 61  PHTKCKLRLLKLERVKDYLLMEEEFVANQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 447

BLAST of HG10006253 vs. ExPASy TrEMBL
Match: A0A6J1EPM8 (26S proteasome regulatory subunit 4 homolog B-like OS=Cucurbita moschata OX=3662 GN=LOC111436236 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.0e-246
Identity = 441/447 (98.66%), Postives = 446/447 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAA+RLPTVT
Sbjct: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAASRLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           PHTKCKLRLLKL+RVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE
Sbjct: 61  PHTKCKLRLLKLDRVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MK+EKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKIEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADD SPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDHSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTSRMTLA+DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSRMTLAEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTHADFKKAK+KVMFKKKEGVPEGLYM
Sbjct: 421 VTHADFKKAKDKVMFKKKEGVPEGLYM 447

BLAST of HG10006253 vs. TAIR 10
Match: AT4G29040.1 (regulatory particle AAA-ATPase 2A )

HSP 1 Score: 815.5 bits (2105), Expect = 2.2e-236
Identity = 422/447 (94.41%), Postives = 434/447 (97.09%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQG  GGLNRQ    GDRK D  +KK+KKFEPAAPP+RVGRKQRKQKGP+AAARLPTVT
Sbjct: 1   MGQGPSGGLNRQ----GDRKPDGGDKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           P TKCKLRLLKLER+KDYLLMEEEFV NQERLKPQEEK EEDRSKVDDLRG+PMSVGNLE
Sbjct: 61  PSTKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGL+AQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTS+MTL++DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSKMTLSEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTH DFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHPDFKKAKEKVMFKKKEGVPEGLYM 443

BLAST of HG10006253 vs. TAIR 10
Match: AT2G20140.1 (AAA-type ATPase family protein )

HSP 1 Score: 813.9 bits (2101), Expect = 6.3e-236
Identity = 422/447 (94.41%), Postives = 433/447 (96.87%), Query Frame = 0

Query: 1   MGQGTPGGLNRQGGLPGDRKQDDPNKKDKKFEPAAPPSRVGRKQRKQKGPDAAARLPTVT 60
           MGQG  GGLNRQ    GDRK D   KK+KKFEPAAPP+RVGRKQRKQKGP+AAARLPTVT
Sbjct: 1   MGQGPSGGLNRQ----GDRKPDGGEKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVT 60

Query: 61  PHTKCKLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLE 120
           P TKCKLRLLKLER+KDYLLMEEEFV NQERLKPQEEK EEDRSKVDDLRG+PMSVGNLE
Sbjct: 61  PSTKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLE 120

Query: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSV 180
           ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVSV
Sbjct: 121 ELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSV 180

Query: 181 MKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240
           MKVEKAPLESYADIGGL+AQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT
Sbjct: 181 MKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKT 240

Query: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300
           LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK
Sbjct: 241 LLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTK 300

Query: 301 RYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360
           RYDA+SGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF
Sbjct: 301 RYDANSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEF 360

Query: 361 PLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420
           PLPDIKTRRRIFQIHTS+MTLA+DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK
Sbjct: 361 PLPDIKTRRRIFQIHTSKMTLAEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMK 420

Query: 421 VTHADFKKAKEKVMFKKKEGVPEGLYM 448
           VTH DFKKAKEKVMFKKKEGVPEGLYM
Sbjct: 421 VTHVDFKKAKEKVMFKKKEGVPEGLYM 443

BLAST of HG10006253 vs. TAIR 10
Match: AT5G58290.1 (regulatory particle triple-A ATPase 3 )

HSP 1 Score: 378.3 bits (970), Expect = 8.8e-105
Identity = 185/362 (51.10%), Postives = 270/362 (74.59%), Query Frame = 0

Query: 68  RLLKLERVKDYLLMEEEFVTN-QERLKPQEEKNEEDRSKVDDLRGSPMSVGNLEELIDEN 127
           RL  LER  ++  ++EE+V + Q+ LK +  + +E+  ++  +   P+ +G   E++D+N
Sbjct: 33  RLKSLERQLEFTDIQEEYVKDEQKNLKRELLRAQEEVKRIQSV---PLVIGQFMEMVDQN 92

Query: 128 HAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMKVEKA 187
           + IV S+ G  YYV ILS ++++ L+P  ++ +H    ++V +L  E D  +S++   + 
Sbjct: 93  NGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEK 152

Query: 188 PLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAV 247
           P  SY DIGG D Q QEI+EAVELPLTH ELY+ IGI PP+GV+LYG PGTGKT+LAKAV
Sbjct: 153 PDVSYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKAV 212

Query: 248 ANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHS 307
           AN T+A F+RVVGSE +QKYLG+GP++VR++FR+A + +P+I+FIDE+DA+ T R+DA +
Sbjct: 213 ANHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEVDAIATARFDAQT 272

Query: 308 GGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIK 367
           G +RE+QR ++ELLNQ+DGFD   +VKVI+ATNR ++LDPALLRPGR+DRKIEFPLPD +
Sbjct: 273 GADREVQRILMELLNQMDGFDQTVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRR 332

Query: 368 TRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHADF 427
            +R +FQ+ TS+M L+D+V+LE++V   D+ S A+I AIC EAG+ A+R+ R  +   DF
Sbjct: 333 QKRLVFQVCTSKMNLSDEVDLEDYVSRPDKISAAEIAAICQEAGMHAVRKNRYVILPKDF 391

Query: 428 KK 429
           +K
Sbjct: 393 EK 391

BLAST of HG10006253 vs. TAIR 10
Match: AT1G09100.1 (26S proteasome AAA-ATPase subunit RPT5B )

HSP 1 Score: 332.4 bits (851), Expect = 5.6e-91
Identity = 177/381 (46.46%), Postives = 250/381 (65.62%), Query Frame = 0

Query: 79  LLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLEELID---ENHA------- 138
           +L EE   TN + L+  +EK +E++ K+   +  P  VGN+ E+++   E+ A       
Sbjct: 37  ILKEESQRTNLD-LESVKEKIKENQEKIKLNKQLPYLVGNIVEILEMSPEDDAEEDGANI 96

Query: 139 -----------IVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPM 198
                      ++ +S     ++ ++  VD D L+PG  + ++     ++  L  E D  
Sbjct: 97  DLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDTLKPGDLVGVNKDSYLILDTLPSEYDSR 156

Query: 199 VSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGT 258
           V  M+V++ P E Y DIGGL+ QIQE+ EA+ LP+TH E +E +GI+PPKGV+LYG PGT
Sbjct: 157 VKAMEVDEKPTEDYNDIGGLEKQIQELVEAIVLPMTHKEQFEKLGIRPPKGVLLYGPPGT 216

Query: 259 GKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAV 318
           GKTL+A+A A  T+ATFL++ G +L+Q ++GDG KLVR+ F +A + SP I+FIDEIDA+
Sbjct: 217 GKTLMARACAAQTNATFLKLAGPQLVQMFIGDGAKLVRDAFLLAKEKSPCIIFIDEIDAI 276

Query: 319 GTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRK 378
           GTKR+D+   G+RE+QRTMLELLNQLDGF S   +KVI ATNR + LDPAL+R GR+DRK
Sbjct: 277 GTKRFDSEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRK 336

Query: 379 IEFPLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRER 438
           IEFP P  + R RI QIH+ +M +  DVN EE   + D+F+GA +KA+C EAG+LALR  
Sbjct: 337 IEFPHPTEEARGRILQIHSRKMNVNADVNFEELARSTDDFNGAQLKAVCVEAGMLALRRD 396

BLAST of HG10006253 vs. TAIR 10
Match: AT3G05530.1 (regulatory particle triple-A ATPase 5A )

HSP 1 Score: 331.6 bits (849), Expect = 9.5e-91
Identity = 173/381 (45.41%), Postives = 247/381 (64.83%), Query Frame = 0

Query: 79  LLMEEEFVTNQERLKPQEEKNEEDRSKVDDLRGSPMSVGNLEELIDENH----------- 138
           +L E+   TN E     +EK +E++ K+   +  P  VGN+ E+++ N            
Sbjct: 38  ILKEDAQRTNLE-CDSYKEKIKENQEKIKLNKQLPYLVGNIVEILEMNPEDDAEEDGANI 97

Query: 139 ----------AIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPM 198
                      ++ +S     ++ ++  VD D L+PG  + ++     ++  L  E D  
Sbjct: 98  DLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDSLKPGDLVGVNKDSYLILDTLPSEYDSR 157

Query: 199 VSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGT 258
           V  M+V++ P E Y DIGGL+ QIQE+ EA+ LP+TH E +E +G++PPKGV+LYG PGT
Sbjct: 158 VKAMEVDEKPTEDYNDIGGLEKQIQELVEAIVLPMTHKERFEKLGVRPPKGVLLYGPPGT 217

Query: 259 GKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAV 318
           GKTL+A+A A  T+ATFL++ G +L+Q ++GDG KLVR+ F++A + +P I+FIDEIDA+
Sbjct: 218 GKTLMARACAAQTNATFLKLAGPQLVQMFIGDGAKLVRDAFQLAKEKAPCIIFIDEIDAI 277

Query: 319 GTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRK 378
           GTKR+D+   G+RE+QRTMLELLNQLDGF S   +KVI ATNR + LDPAL+R GR+DRK
Sbjct: 278 GTKRFDSEVSGDREVQRTMLELLNQLDGFSSDERIKVIAATNRADILDPALMRSGRLDRK 337

Query: 379 IEFPLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRER 438
           IEFP P  + R RI QIH+ +M +  DVN EE   + D+F+GA +KA+C EAG+LALR  
Sbjct: 338 IEFPHPTEEARARILQIHSRKMNVHPDVNFEELARSTDDFNGAQLKAVCVEAGMLALRRD 397

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004149071.12.3e-248100.0026S proteasome regulatory subunit 4 homolog B [Cucumis sativus] >XP_008452203.1 ... [more]
XP_022153378.18.8e-24899.7826S proteasome regulatory subunit 4 homolog B-like [Momordica charantia][more]
KAG7029796.16.3e-24698.6626S proteasome regulatory subunit 4-like B [Cucurbita argyrosperma subsp. argyro... [more]
XP_022929739.16.3e-24698.6626S proteasome regulatory subunit 4 homolog B-like [Cucurbita moschata] >KAG6598... [more]
XP_022997411.11.8e-24598.4326S proteasome regulatory subunit 4 homolog B-like [Cucurbita maxima] >XP_023546... [more]
Match NameE-valueIdentityDescription
Q9SZD43.0e-23594.4126S proteasome regulatory subunit 4 homolog A OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SL678.9e-23594.4126S proteasome regulatory subunit 4 homolog B OS=Arabidopsis thaliana OX=3702 GN... [more]
P464663.2e-23292.6326S proteasome regulatory subunit 4 homolog OS=Oryza sativa subsp. japonica OX=3... [more]
P486011.5e-19779.5926S proteasome regulatory subunit 4 OS=Drosophila melanogaster OX=7227 GN=Rpt2 P... [more]
P621911.1e-19577.1826S proteasome regulatory subunit 4 OS=Homo sapiens OX=9606 GN=PSMC1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CKV41.1e-248100.0026S proteasome regulatory subunit 4-like protein A OS=Cucumis melo var. makuwa O... [more]
A0A0A0LH671.1e-248100.00AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G049870 PE=3 SV... [more]
A0A1S3BUF21.1e-248100.00LOW QUALITY PROTEIN: 26S proteasome regulatory subunit 4 homolog B-like OS=Cucum... [more]
A0A6J1DHA64.3e-24899.7826S proteasome regulatory subunit 4 homolog B-like OS=Momordica charantia OX=367... [more]
A0A6J1EPM83.0e-24698.6626S proteasome regulatory subunit 4 homolog B-like OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT4G29040.12.2e-23694.41regulatory particle AAA-ATPase 2A [more]
AT2G20140.16.3e-23694.41AAA-type ATPase family protein [more]
AT5G58290.18.8e-10551.10regulatory particle triple-A ATPase 3 [more]
AT1G09100.15.6e-9146.4626S proteasome AAA-ATPase subunit RPT5B [more]
AT3G05530.19.5e-9145.41regulatory particle triple-A ATPase 5A [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 225..364
e-value: 3.6E-20
score: 83.0
IPR032501Proteasomal ATPase OB C-terminal domainPFAMPF16450Prot_ATP_ID_OBcoord: 116..169
e-value: 4.2E-10
score: 39.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 182..362
e-value: 3.2E-68
score: 231.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 190..440
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 229..362
e-value: 3.4E-40
score: 137.5
IPR00593726S proteasome regulatory subunit P45-likeTIGRFAMTIGR01242TIGR01242coord: 82..434
e-value: 1.2E-140
score: 466.8
IPR041569AAA ATPase, AAA+ lid domainPFAMPF17862AAA_lid_3coord: 384..428
e-value: 2.6E-10
score: 40.0
NoneNo IPR availableGENE3D2.40.50.140coord: 114..181
e-value: 1.8E-24
score: 87.3
NoneNo IPR availableGENE3D1.10.8.60coord: 367..441
e-value: 6.9E-26
score: 91.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..109
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..59
NoneNo IPR availablePANTHERPTHR2307326S PROTEASOME REGULATORY SUBUNITcoord: 35..447
NoneNo IPR availablePANTHERPTHR23073:SF111BNAA08G13550D PROTEINcoord: 35..447
NoneNo IPR availableCDDcd00009AAAcoord: 195..362
e-value: 1.41835E-26
score: 102.609
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 332..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10006253.1HG10006253.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901800 positive regulation of proteasomal protein catabolic process
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0030163 protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0008540 proteasome regulatory particle, base subcomplex
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0036402 proteasome-activating activity