HG10006237 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10006237
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description(R)-mandelonitrile lyase 1-like
LocationChr07: 16301897 .. 16304584 (+)
RNA-Seq ExpressionHG10006237
SyntenyHG10006237
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCATTCTAGGGGTACCATTCTCATACTCAGCCTTATAATCTCTATATTTCAATTAGCAGTTCTCTCCTCACATGCAATTCCCAATCAAGGTAAAATTTTGCCTCTCAAATTACAAACTTTTCTCTTAATTAATGAATTCTTATTGATCTAAAATTCCTTTTTTCAGATTTTAGCTACATGAAGTTTGTACATAATGCCAGCGATTTGCCAGCAAAAGAAGAATATGATTACATAATAATAGGAGGAGGAACAGCAGGTTGTCCATTAGCTACAACATTATCATCAAATTTCTCAGTCCTCCTTCTTGAAAGAGGCAGTGATCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAATGTTTTTGCTGCTGGAGATGATGGTAAAAATCCCTTCCAACGCTTCACCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGAGTCCTTGGCGGTGGAAGTATGGTTAATGCTGGCTTTTACTCGAGAGGCCATCGACAGTTCTTTGAAACAGCAGGTGTTGATTGGGATATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGACTGTGACATCTCGACCAATTTTGAATGCTTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAGCTGGTGTTTTACCTGATAATGGGTTTGATTTGAGGCATCTTGTCGGAACTAAAACTGGAGGTTCCATCTTTGATGACAAAGGAAACAGACATGGAGCTGTTGAGCTTCTCAATAAGGCTAAACCCACCAATATTAAAGTTGCAATTGAAGCCACAGTCCAAAGAATCCTCTTTTCTGGTTAGTCCTAGTAAATATTCTATTTTAATTATATTTTTTGTATTTATTTTTTTGAGTTTTTTTTTTTTTTTTTTTTTTTTACGGATGACCCATTCAGGGGTCCCTACAAATATATGAAATCTAACCTTATGCTTACGTCATAACGCACTACTGCATTTTTTTAACATATCGTGTTAGGGATGACCTTGACCATTGTGTATTGTAGCCTAACGCGATGACCATTATCTTTATCATGATTTTTATTTTTCTATTGTGTTTTCCTCATTTTTCTCACAGTTGTTTGTTTTTTAGGTGAGAAAAATGAATATCGTGATAGAGATGATCATCCGTTAGGACTACATGGTTATCTCTAACGAAATACATTATAAAATGTTCTCCTTTTTTCTCATAATTTGTTAGGTTTTTCAGAAAAATATGAAAATCGTGATAGAGATGGTCATCGCGTTATGCTACGATAAACATGGTCATCTCTAACGCAATAAGTTATATAAAATGCGATAATACCTTATGACGTAAGCAGAAGATTATATTTCATACGTTTGTGAGGGATGGATCATCAATCATAAACACCCTCCTCAAAGAGCCATTCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTGATTTTTTATTCCTAAACTTCGGTAACGTTTATTTTAGTGCTTGATCCATAAATCATTTTGATTGTGATATTATTTTGTAATTTAATATAAGGAAATTTTTAAAAATAAAAAAATAAAGAAACTATTTACACAAAATAGCAAAATTTTTAGATAGTTGTGATAGACACTAGTAGAAGTCTATCAGGGTATATAAATGATAGAAATGACAGAACTTATCACTGATAGATGCTGATAGAAGTCTATCAATGTTTATTAGTGTTTTTTTTGCTATTTTCTGTAAATAATTTGACATTTTTTTCTATATGGAAAAATTTTCCTTTAATATATGCATGAATTTTTTTTTATTTATTTTTTTTTTTTTTTTTTTGCTCATTCTGAACTCAGGTTTAACTGCAAGTGGGGTTTTGTATTCTGATTCAAAAGGAAAACTACATAGAGCATTCATCCGTGAGAAAGGTGAAATAATTGTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTTATCTTTCATCTTTAAAATTACCTGTCGTTCTTCACCAACCACATATCGGTGAATTCATGTCGGACAATCCCCGTTTTGGGACAAACATCATCCTTCCATTCAAATTGCTTCCTTCCTCTGTAAAAGTTGTTGGAATTTTAGAAGACAATATCTATATACAATCTTTTGCCAGCCCTTCACCAATTTTAGTTCCCCCAATTTTCAGTCTTCTTCCTCCTCAATCCACTTCCATCATCCCCAGCTTAGCCATGTTTGTTGGAAAATTCTCGGAGGTGCATTCTGAAGGCTCACTCCGATTGAATTCCTCCACTAATGTGAAGAAGAGCCCCATTGTTCGATTCAATTACTATTCACATCCTGATGATCTTGCTCGATGTGTTAGTGGAGTAAGAAAAATGGGAGATTTGCTTAAAACCCAAACCATGGAAAAGATTAAGACCCAAGATTTGGAGGGTAATAAAGTATTTGAGTTTTTGGGGCTTCCCTTGCCGGAAAATTTGTTGAATGATAGCTCCGTAGAAGAGTATTGTCATAAGACAGTGACAACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGGTAATTATAAAGTCATCGGAATAAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCTGAGTCACCGGGAACTAATCCTATGGCCACCCTCATGATGCTCGGCCGGTGA

mRNA sequence

ATGGAGCATTCTAGGGGTACCATTCTCATACTCAGCCTTATAATCTCTATATTTCAATTAGCAGTTCTCTCCTCACATGCAATTCCCAATCAAGATTTTAGCTACATGAAGTTTGTACATAATGCCAGCGATTTGCCAGCAAAAGAAGAATATGATTACATAATAATAGGAGGAGGAACAGCAGGTTGTCCATTAGCTACAACATTATCATCAAATTTCTCAGTCCTCCTTCTTGAAAGAGGCAGTGATCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAATGTTTTTGCTGCTGGAGATGATGGTAAAAATCCCTTCCAACGCTTCACCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGAGTCCTTGGCGGTGGAAGTATGGTTAATGCTGGCTTTTACTCGAGAGGCCATCGACAGTTCTTTGAAACAGCAGGTGTTGATTGGGATATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGACTGTGACATCTCGACCAATTTTGAATGCTTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAGCTGGTGTTTTACCTGATAATGGGTTTGATTTGAGGCATCTTGTCGGAACTAAAACTGGAGGTTCCATCTTTGATGACAAAGGAAACAGACATGGAGCTGTTGAGCTTCTCAATAAGGCTAAACCCACCAATATTAAAGTTGCAATTGAAGCCACAGTCCAAAGAATCCTCTTTTCTGGTTTAACTGCAAGTGGGGTTTTGTATTCTGATTCAAAAGGAAAACTACATAGAGCATTCATCCGTGAGAAAGGTGAAATAATTGTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTTATCTTTCATCTTTAAAATTACCTGTCGTTCTTCACCAACCACATATCGGTGAATTCATGTCGGACAATCCCCGTTTTGGGACAAACATCATCCTTCCATTCAAATTGCTTCCTTCCTCTGTAAAAGTTGTTGGAATTTTAGAAGACAATATCTATATACAATCTTTTGCCAGCCCTTCACCAATTTTAGTTCCCCCAATTTTCAGTCTTCTTCCTCCTCAATCCACTTCCATCATCCCCAGCTTAGCCATGTTTGTTGGAAAATTCTCGGAGGTGCATTCTGAAGGCTCACTCCGATTGAATTCCTCCACTAATGTGAAGAAGAGCCCCATTGTTCGATTCAATTACTATTCACATCCTGATGATCTTGCTCGATGTGTTAGTGGAGTAAGAAAAATGGGAGATTTGCTTAAAACCCAAACCATGGAAAAGATTAAGACCCAAGATTTGGAGGGTAATAAAGTATTTGAGTTTTTGGGGCTTCCCTTGCCGGAAAATTTGTTGAATGATAGCTCCGTAGAAGAGTATTGTCATAAGACAGTGACAACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGGTAATTATAAAGTCATCGGAATAAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCTGAGTCACCGGGAACTAATCCTATGGCCACCCTCATGATGCTCGGCCGGTGA

Coding sequence (CDS)

ATGGAGCATTCTAGGGGTACCATTCTCATACTCAGCCTTATAATCTCTATATTTCAATTAGCAGTTCTCTCCTCACATGCAATTCCCAATCAAGATTTTAGCTACATGAAGTTTGTACATAATGCCAGCGATTTGCCAGCAAAAGAAGAATATGATTACATAATAATAGGAGGAGGAACAGCAGGTTGTCCATTAGCTACAACATTATCATCAAATTTCTCAGTCCTCCTTCTTGAAAGAGGCAGTGATCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAATGTTTTTGCTGCTGGAGATGATGGTAAAAATCCCTTCCAACGCTTCACCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGAGTCCTTGGCGGTGGAAGTATGGTTAATGCTGGCTTTTACTCGAGAGGCCATCGACAGTTCTTTGAAACAGCAGGTGTTGATTGGGATATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGACTGTGACATCTCGACCAATTTTGAATGCTTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAGCTGGTGTTTTACCTGATAATGGGTTTGATTTGAGGCATCTTGTCGGAACTAAAACTGGAGGTTCCATCTTTGATGACAAAGGAAACAGACATGGAGCTGTTGAGCTTCTCAATAAGGCTAAACCCACCAATATTAAAGTTGCAATTGAAGCCACAGTCCAAAGAATCCTCTTTTCTGGTTTAACTGCAAGTGGGGTTTTGTATTCTGATTCAAAAGGAAAACTACATAGAGCATTCATCCGTGAGAAAGGTGAAATAATTGTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTTATCTTTCATCTTTAAAATTACCTGTCGTTCTTCACCAACCACATATCGGTGAATTCATGTCGGACAATCCCCGTTTTGGGACAAACATCATCCTTCCATTCAAATTGCTTCCTTCCTCTGTAAAAGTTGTTGGAATTTTAGAAGACAATATCTATATACAATCTTTTGCCAGCCCTTCACCAATTTTAGTTCCCCCAATTTTCAGTCTTCTTCCTCCTCAATCCACTTCCATCATCCCCAGCTTAGCCATGTTTGTTGGAAAATTCTCGGAGGTGCATTCTGAAGGCTCACTCCGATTGAATTCCTCCACTAATGTGAAGAAGAGCCCCATTGTTCGATTCAATTACTATTCACATCCTGATGATCTTGCTCGATGTGTTAGTGGAGTAAGAAAAATGGGAGATTTGCTTAAAACCCAAACCATGGAAAAGATTAAGACCCAAGATTTGGAGGGTAATAAAGTATTTGAGTTTTTGGGGCTTCCCTTGCCGGAAAATTTGTTGAATGATAGCTCCGTAGAAGAGTATTGTCATAAGACAGTGACAACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGGTAATTATAAAGTCATCGGAATAAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCTGAGTCACCGGGAACTAATCCTATGGCCACCCTCATGATGCTCGGCCGGTGA

Protein sequence

MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILVPPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR
Homology
BLAST of HG10006237 vs. NCBI nr
Match: XP_038889314.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 973.0 bits (2514), Expect = 1.0e-279
Identity = 483/530 (91.13%), Postives = 504/530 (95.09%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 60
           MEHS+ TILILSL+ISI QL VL+ +AIP +D  YMKFVH ASDLPAKEEYDYIIIGGGT
Sbjct: 1   MEHSKATILILSLMISISQLGVLTYNAIPIKDVRYMKFVHKASDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 120
           AGCPLA TLSS FSVL+LERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRF SEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLVLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFISEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 180
           NIR RVLGGGSMVNAGFYSRGH+QFFETAGVDWDMELVEKAY+WVEETV SRPILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVASRPILNAWQS 180

Query: 181 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 240
           AFRSALLEAGV+PDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKA+PTNIKVAIEAT
Sbjct: 181 AFRSALLEAGVVPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPTNIKVAIEAT 240

Query: 241 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQ+ILFSGL+A GVLYSDSKGKLHRAFIR+KGEIIVSAGAIGSPQLLLLSGVGPKS+LSS
Sbjct: 241 VQKILFSGLSARGVLYSDSKGKLHRAFIRKKGEIIVSAGAIGSPQLLLLSGVGPKSHLSS 300

Query: 301 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 360
           LKLPVVLHQP++GEFMSDNPRFGTNIILPF+L+PSSVKVVGIL+DNIYIQSFASPSP   
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420
           PP FSLLPPQ  SI+PSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 480
           RCV GVRKMGDLLKTQTMEKIKT DLEGNK F+FLGLPLPENL NDSSV EYC KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVGEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of HG10006237 vs. NCBI nr
Match: XP_011650116.2 ((R)-mandelonitrile lyase 1 [Cucumis sativus])

HSP 1 Score: 967.6 bits (2500), Expect = 4.3e-278
Identity = 478/530 (90.19%), Postives = 507/530 (95.66%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 60
           MEHSR TILILSL+ISIFQL + SSHAIPNQD SYMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 120
           AGCPLATTLSS FSVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRF SEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 180
           NIRGRVLGGGSMVNAGFYSRGHR+FF +AGVDWDMELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 240
           AFRS+LLE GV+PDNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVAIEAT
Sbjct: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 241 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRILFSGL+A+GVLYSDSKGKLHRA IR+KGEIIVSAGAIGSPQLLLLSG+GPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 301 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 360
           LKLPVVLHQP++G+ MSDNPRFGTNII+PF +LPSSVKVVGIL+DNIYIQS ASP PIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 361 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420
           P IFSLLPPQ+TSIIP+LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDL 
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420

Query: 421 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 480
           RCV GVRKMGDLLKT+TMEKIKT++LEGNK FEFLG+PLPENL NDSSVEEYC KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of HG10006237 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 963.4 bits (2489), Expect = 8.2e-277
Identity = 474/530 (89.43%), Postives = 500/530 (94.34%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 60
           MEHSR TILILSL+ISIF L +LSSHAIPNQD  YMKFVH+A+DLPAKEEYDYIIIGGGT
Sbjct: 1   MEHSRSTILILSLMISIFHLGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 120
           AGCPL++TLSS FSVLLLERGSDPNKYPSVLNEQGLLN FAA DDGKNPFQ F SEDGVE
Sbjct: 61  AGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 180
           NIRGRVLGGGSMVN GFYSRGH+QFFETAGVDWDMELVEKAY+WVEETV S+PILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 240
           AFRSA L+AGV+PDNGFDLRH VGTK GGSIFD+KGNRHGAVELLNKA PTNIKV IEAT
Sbjct: 181 AFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIEAT 240

Query: 241 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRILFSGL+A GVLYSDSKGKLHRAFIR++GEIIVSAGA+GSPQLLLLSGVGPKS+LSS
Sbjct: 241 VQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHLSS 300

Query: 301 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 360
           LKLPVVLHQP++GEFMSDNPRFGTNIILPF+L+PSSVKVVGIL+DNIYIQSFASPSP   
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420
           PP FSLLPPQ  SI+PSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 480
           RCV GVRKMGDLLKTQTMEKIKT DLEGNK F+FLGLPLPENL NDSSVEEYC KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGCLVGKVVDGNYKVIGI+NLRVVDGSTF +SPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFCDSPGTNPMATLMMLGR 530

BLAST of HG10006237 vs. NCBI nr
Match: KAE8651660.1 (hypothetical protein Csa_021193 [Cucumis sativus])

HSP 1 Score: 948.0 bits (2449), Expect = 3.6e-272
Identity = 466/517 (90.14%), Postives = 495/517 (95.74%), Query Frame = 0

Query: 14  IISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNF 73
           +ISIFQL + SSHAIPNQD SYMKFVHNA+DLP KEEYDYIIIGGGTAGCPLATTLSS F
Sbjct: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60

Query: 74  SVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMV 133
           SVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRF SEDGVENIRGRVLGGGSMV
Sbjct: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120

Query: 134 NAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLP 193
           NAGFYSRGHR+FF +AGVDWDMELVEKAYEWVEETV S+PILNAWQSAFRS+LLE GV+P
Sbjct: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180

Query: 194 DNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILFSGLTASG 253
           DNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVAIEATVQRILFSGL+A+G
Sbjct: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240

Query: 254 VLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKLPVVLHQPHIG 313
           VLYSDSKGKLHRA IR+KGEIIVSAGAIGSPQLLLLSG+GPKS+LSSLKLPVVLHQP++G
Sbjct: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300

Query: 314 EFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILVPPIFSLLPPQSTS 373
           + MSDNPRFGTNII+PF +LPSSVKVVGIL+DNIYIQS ASP PIL+P IFSLLPPQ+TS
Sbjct: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSLLPPQATS 360

Query: 374 IIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLARCVSGVRKMGDLL 433
           IIP+LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDL RCV GVRKMGDLL
Sbjct: 361 IIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420

Query: 434 KTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTYWHYHGGCLVGKVV 493
           KT+TMEKIKT++LEGNK FEFLG+PLPENL NDSSVEEYC KTVTTYWHYHGGCLVGKVV
Sbjct: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480

Query: 494 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 517

BLAST of HG10006237 vs. NCBI nr
Match: XP_008452041.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 933.7 bits (2412), Expect = 6.9e-268
Identity = 463/530 (87.36%), Postives = 496/530 (93.58%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 60
           MEH R TILILSL+ISIFQL + SS AIPNQD  YMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 120
           AGCPLATTLSS FSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRF SEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 180
           NIR RVLGGGSMVNAGFYSRGH++FF +AGVDW+MELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 240
           AFRS+LLE GV+PDNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRILFSGL+A+GVLYSDSKGKLH+AFIR KGEII+SAGAIGSPQLLLLSG+GPKSYLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 360
           LKLPVVLHQP++G+ MSDNPRFGTNIILPF +LPSSVKVVGIL+DNIYIQS ASP PILV
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420
           PPIFS L  Q TSI P+ A+FVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDL 
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 480
           RCV GVRK+GDLLKT+TMEKIKTQ+LEGNK FEFLG+PLPENL N S+VEEYC KT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGC+VGKVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 529

BLAST of HG10006237 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 4.8e-155
Identity = 274/528 (51.89%), Postives = 367/528 (69.51%), Query Frame = 0

Query: 8   ILILSLIISIFQLAVLSSHA-IPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLA 67
           +L+L L + + Q + + S A   N DFSY++F ++A+DL  +  YDY+I+GGGT+GCPLA
Sbjct: 10  LLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLA 69

Query: 68  TTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRV 127
            TLS  + VL+LERGS P  YP+VL   G +      DDGK P +RF SEDG++N+RGRV
Sbjct: 70  ATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRV 129

Query: 128 LGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSAL 187
           LGG SM+NAG Y+R +   +  +GVDWDM+LV K YEWVE+T+  +P    WQS   +A 
Sbjct: 130 LGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAF 189

Query: 188 LEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILF 247
           LEAGV P++GF L H  GT+  GS FD+KG RH A ELLNK    N++V + A+V++I+F
Sbjct: 190 LEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIF 249

Query: 248 S---GLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKL 307
           S   GLTA+GV+Y DS G  HRAF+R KGE+IVSAG IG+PQLLLLSGVGP+SYLSSL +
Sbjct: 250 SNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNI 309

Query: 308 PVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILVPPI 367
           PVVL  P++G+F+ DNPR   NI+ P  + P+ V V+GI  D  + Q   S  P   PP 
Sbjct: 310 PVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTPP- 369

Query: 368 FSLLPPQSTSIIPS-LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLARC 427
           FS  P  S  +  S  A F  K +   S GSL L SS+NV+ SP V+FNYYS+P DL+ C
Sbjct: 370 FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHC 429

Query: 428 VSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTYWH 487
           VSG++K+G+LL T  ++  K +DL G + F  LG+PLP++  +D++ E +C ++V +YWH
Sbjct: 430 VSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWH 489

Query: 488 YHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           YHGGCLVGKV+DG+++V GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 490 YHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGR 534

BLAST of HG10006237 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.3e-152
Identity = 270/537 (50.28%), Postives = 368/537 (68.53%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAI---PNQDFSYMKFVHNASDLPAKEEYDYIIIG 60
           ME S  + ++L L I +  L     H++    + DFSY+ F ++A+DL  +  YDY+I+G
Sbjct: 1   MEKSTMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVG 60

Query: 61  GGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSED 120
           GGT+GCPLA TLS  + VL+LERGS P  YP+VL   G +      DDGK P +RF SED
Sbjct: 61  GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNA 180
           G++N+RGRVLGG S++NAG Y+R +   +  +GVDWDM+LV + YEWVE+T+  +P   +
Sbjct: 121 GIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQS 180

Query: 181 WQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAI 240
           WQS  ++A LEAGV P++GF L H  GT+  GS FD+KG RH A ELLNK    N++V +
Sbjct: 181 WQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGV 240

Query: 241 EATVQRILFS---GLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGP 300
            A+V++I+FS   GLTA+GV+Y DS G  H+AF+R KGE+IVSAG IG+PQLLLLSGVGP
Sbjct: 241 HASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGP 300

Query: 301 KSYLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFAS 360
           +SYLSSL +PVVL  P++G+F+ DNPR   NI+ P  + P+ V V+GI  D  + Q   S
Sbjct: 301 ESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFS 360

Query: 361 PSPILVPPIFSLLPPQSTSIIPS-LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYY 420
             P   PP F   P  S  +  S  A F  K +   S GSL L SS+NV+ SP V+FNYY
Sbjct: 361 SLPFTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYY 420

Query: 421 SHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYC 480
           S+  DL+ CVSG++K+G+LL T  ++  K +DL G + F  LG+PLP++  +D++ E +C
Sbjct: 421 SNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFC 480

Query: 481 HKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
            ++V +YWHYHGGCLVGKV+DG+++V GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 481 RESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGR 534

BLAST of HG10006237 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 3.0e-149
Identity = 263/529 (49.72%), Postives = 363/529 (68.62%), Query Frame = 0

Query: 8   ILILSLIISIFQLAVLSSHA-IPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLA 67
           +L+L + +   Q + + S A   + DFSY+ FV++A+D   +  YDYII+GGGTAGCPLA
Sbjct: 10  LLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLA 69

Query: 68  TTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRV 127
            TLS+N+SVL+LERGS P +YP++L   G +      DDGK P +RF SEDG++N+RGRV
Sbjct: 70  ATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRV 129

Query: 128 LGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSAL 187
           LGG SM+NAG Y R +  FF   G++WDM+LV + YEWVE+T+   P    WQ+   +A 
Sbjct: 130 LGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAY 189

Query: 188 LEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILF 247
           LEAG+LP+NGF + HL GT+  GS FD+ G RH + ELLNK  P N++VA++A V++I+F
Sbjct: 190 LEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIF 249

Query: 248 ----SGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLK 307
               SG+TA GV+Y+DS G  H+AF+R +GE+I+SAG IGSPQLLLLSGVGP+SYL+SL 
Sbjct: 250 SSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLN 309

Query: 308 LPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILVPP 367
           + VV   P++G+++ DNPR   NI+ P  +  S+V V+GI  D  + Q   S  P   PP
Sbjct: 310 ISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLPFDTPP 369

Query: 368 IFSLLPPQSTSI-IPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLAR 427
            FS  P  S  +   + A  V K     S G++ LNSS++V+  P V+FNYYS+  DL+ 
Sbjct: 370 -FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSH 429

Query: 428 CVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTYW 487
           CVSG++K+G++L T  +E  K +DL G   F  LG+PLPEN  +D++ E +C ++V +YW
Sbjct: 430 CVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVASYW 489

Query: 488 HYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           HYHGGCLVGKV+D  ++V GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 490 HYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGR 535

BLAST of HG10006237 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.2e-148
Identity = 268/539 (49.72%), Postives = 359/539 (66.60%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPN----QDFSYMKFVHNASDLPAKEEYDYIII 60
           ME S  + ++L L + +  L     H++ N     DF Y+KFV+NA DL  +  YDYII+
Sbjct: 1   MEKSTMSAVVLVLNLLVLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIV 60

Query: 61  GGGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSE 120
           GGGT+GCPLA TLS+N+SVL+LERG+   +YP+ L   G        DDGK P +RF SE
Sbjct: 61  GGGTSGCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSE 120

Query: 121 DGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILN 180
           DG++N+R R+LGG +++NAG Y+R +  F+  +GV+WD++LV +AYEWVE+ +  +P   
Sbjct: 121 DGIDNVRSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQ 180

Query: 181 AWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVA 240
           +WQS   +A LEAGV PDNGF L H  GT+  GS FD+ G RH + ELLNK  P N+KVA
Sbjct: 181 SWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVA 240

Query: 241 IEATVQRILF----SGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGV 300
           +EA VQ+I+F    SGLTA GV+Y+DS G  HRA +  KGE+I+SAG +G+PQLLLLSGV
Sbjct: 241 VEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGV 300

Query: 301 GPKSYLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSF 360
           GP+SYL+SL + VV   P++G++++DNPR   NI+ P  + PS+V V+GI  D  + Q  
Sbjct: 301 GPESYLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQCS 360

Query: 361 ASPSPILVPPIFSLLPPQSTSI-IPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFN 420
            S  P   PP FSL P  S  +   + A  V K     S GSL L SS+NV  +P V+FN
Sbjct: 361 LSSLPFDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFN 420

Query: 421 YYSHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEE 480
           Y S P DL  CVSG++K+G  L T  ++  K  DL G   F  LG PLPEN  +D++ E+
Sbjct: 421 YCSDPVDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEK 480

Query: 481 YCHKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           +C  TV +YWHYHGG +VGKV+DGN++V GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 481 FCRDTVASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLGR 536

BLAST of HG10006237 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 4.1e-146
Identity = 263/538 (48.88%), Postives = 357/538 (66.36%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPN---QDFSYMKFVHNASDLPAKEEYDYIIIG 60
           ME S  ++++  L + +  L     H++ N    DFSY+KFV+NA+D   +  YDYI+IG
Sbjct: 1   MEKSTMSVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIG 60

Query: 61  GGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSED 120
           GGT+GCPLA TLS  + VLLLERG+   +YP+ L   G        DDGK P +RF SED
Sbjct: 61  GGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNA 180
           G++N+R R+LGG +++NAG Y+R +  F+   G++WD++LV K YEWVE+ +  +P   +
Sbjct: 121 GIDNVRARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQS 180

Query: 181 WQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAI 240
           WQS      LEAG+LPDNGF L H  GT+  GS FD+ G RH A ELLNK  P N+ VA+
Sbjct: 181 WQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAV 240

Query: 241 EATVQRILF----SGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVG 300
           +A+V++ILF    S L+A GV+Y+DS G  H+AF+R  GE+IVSAG IG+PQLLLLSGVG
Sbjct: 241 QASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVG 300

Query: 301 PKSYLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFA 360
           P+SYLSSL + VV   P++G+F+ +NPR   N   P  +  S V V+GI  D  Y Q   
Sbjct: 301 PESYLSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSL 360

Query: 361 SPSPILVPPIFSLLPPQSTSIIPS-LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNY 420
           S  P   PP FSL P  S  +  S  A  V +     S GS+ LNSS++V+ +P ++FNY
Sbjct: 361 SSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNY 420

Query: 421 YSHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEY 480
           YS+  DLA CVSG++K+GDLL+T+ +E  K +D+ G   F +LG+PLPEN  +D+S E +
Sbjct: 421 YSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETF 480

Query: 481 CHKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           C   V +YWHYHGG LVGKV+D +++V+GIK LRVVD STF   P ++P    +MLGR
Sbjct: 481 CLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGR 535

BLAST of HG10006237 vs. ExPASy TrEMBL
Match: A0A0A0LK76 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 8.0e-278
Identity = 477/530 (90.00%), Postives = 507/530 (95.66%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 60
           MEHSR TILILSL+ISIFQL + SSHAIPNQD SYMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 120
           AGCPLATTLSS FSVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRF SEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 180
           NIRGRVLGGGSMVNAGFYSRGHR+FF +AGVDWDMELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 240
           AFRS+LLE GV+PD+GFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVAIEAT
Sbjct: 181 AFRSSLLEGGVVPDSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 241 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRILFSGL+A+GVLYSDSKGKLHRA IR+KGEIIVSAGAIGSPQLLLLSG+GPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 301 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 360
           LKLPVVLHQP++G+ MSDNPRFGTNII+PF +LPSSVKVVGIL+DNIYIQS ASP PIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 361 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420
           P IFSLLPPQ+TSIIP+LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDL 
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420

Query: 421 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 480
           RCV GVRKMGDLLKT+TMEKIKT++LEGNK FEFLG+PLPENL NDSSVEEYC KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of HG10006237 vs. ExPASy TrEMBL
Match: A0A5A7VNU1 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G001020 PE=3 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.4e-268
Identity = 463/530 (87.36%), Postives = 496/530 (93.58%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 60
           MEH R TILILSL+ISIFQL + SS AIPNQD  YMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 120
           AGCPLATTLSS FSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRF SEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 180
           NIR RVLGGGSMVNAGFYSRGH++FF +AGVDW+MELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 240
           AFRS+LLE GV+PDNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRILFSGL+A+GVLYSDSKGKLH+AFIR KGEII+SAGAIGSPQLLLLSG+GPKSYLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 360
           LKLPVVLHQP++G+ MSDNPRFGTNIILPF +LPSSVKVVGIL+DNIYIQS ASP PILV
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420
           PPIFS L  Q TSI P+ A+FVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDL 
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 480
           RCV GVRK+GDLLKT+TMEKIKTQ+LEGNK FEFLG+PLPENL N S+VEEYC KT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGC+VGKVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 529

BLAST of HG10006237 vs. ExPASy TrEMBL
Match: A0A1S3BSB5 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493164 PE=3 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.4e-268
Identity = 463/530 (87.36%), Postives = 496/530 (93.58%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 60
           MEH R TILILSL+ISIFQL + SS AIPNQD  YMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 120
           AGCPLATTLSS FSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRF SEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 180
           NIR RVLGGGSMVNAGFYSRGH++FF +AGVDW+MELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 240
           AFRS+LLE GV+PDNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRILFSGL+A+GVLYSDSKGKLH+AFIR KGEII+SAGAIGSPQLLLLSG+GPKSYLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 360
           LKLPVVLHQP++G+ MSDNPRFGTNIILPF +LPSSVKVVGIL+DNIYIQS ASP PILV
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420
           PPIFS L  Q TSI P+ A+FVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDL 
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 480
           RCV GVRK+GDLLKT+TMEKIKTQ+LEGNK FEFLG+PLPENL N S+VEEYC KT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGC+VGKVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 529

BLAST of HG10006237 vs. ExPASy TrEMBL
Match: A0A5A7VGR6 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G001190 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.1e-263
Identity = 453/530 (85.47%), Postives = 490/530 (92.45%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 60
           MEHSR TILILSL+ISIFQL + SSHAIPNQD SYMKFVHNA+DL  KEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLSTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 120
           AGCPLATTLSS FSVL+LERGSDPNKYP VLNE GL NVF AGDDGKNP QRF SEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLVLERGSDPNKYPFVLNELGLFNVFTAGDDGKNPSQRFISEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 180
           NIRGRVLGGGSMVNAGFYSRGHR+FFE+AGVDWDMELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 240
            FRS+LLE+GV+PDNGFDLRHLVGTKTGGSIFD+ GNRHGAVE LNKA  TNIKVAIEAT
Sbjct: 181 TFRSSLLESGVVPDNGFDLRHLVGTKTGGSIFDNIGNRHGAVEFLNKANTTNIKVAIEAT 240

Query: 241 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 300
           VQRILFSGL+A+GVLYSDSKGKLH+AFIR+KGEII+SAGAIGSPQLLLLSG+GPKSYLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 360
           LKLP+VLHQP++G+ MSDNPRFGT  ILPF +LPSS K VGIL+DNIYIQS AS  PIL+
Sbjct: 301 LKLPLVLHQPYVGQSMSDNPRFGTYFILPFPVLPSSAKAVGILQDNIYIQSIASLLPILI 360

Query: 361 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420
           PPIFS LPP +TSI PSL + +GKFSEVHSEGSLRLNSST+VK+SPIVRFNYYSHPDDL 
Sbjct: 361 PPIFSFLPPGATSIDPSLVVLIGKFSEVHSEGSLRLNSSTDVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 480
           RCV GVRK+GDLLKT+TMEKIKTQ+LE NK FEFLG+PLPENL N S+VEEYC KTV+TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEDNKGFEFLGVPLPENLGNVSAVEEYCKKTVSTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           WHYHGGC+VGKVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 530

BLAST of HG10006237 vs. ExPASy TrEMBL
Match: A0A1S3BTS5 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.3e-259
Identity = 449/531 (84.56%), Postives = 484/531 (91.15%), Query Frame = 0

Query: 1   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 60
           ME  + TILILSL ISIF L +LSS AIPNQD SYMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MERCKVTILILSLTISIFPLQLLSSEAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 120
           AGCPLATTLSS FS+L+LERGSDPNKYPSVLN QG+ N FAAGDDGKNPFQ F SEDGVE
Sbjct: 61  AGCPLATTLSSKFSILVLERGSDPNKYPSVLNAQGINNAFAAGDDGKNPFQYFVSEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPIL-NAWQ 180
           NIRGRVLGGGSMVNAGFYSRGHR+FFE AGVDWDMELVEKAYEWVEETV S+PIL NAWQ
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFENAGVDWDMELVEKAYEWVEETVVSQPILNNAWQ 180

Query: 181 SAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEA 240
           +AFRSALLE GV+PDNGFDLRHLVGTK GGSIFD+KGNRHGAVELLNKA PTNIKVAI+A
Sbjct: 181 AAFRSALLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVAIQA 240

Query: 241 TVQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLS 300
           TVQ+ILFSGL+A+GV YSDSKGKLH A I + GEIIVSAGAIGSPQLLLLSG+GPKS+LS
Sbjct: 241 TVQKILFSGLSATGVSYSDSKGKLHTASIHKNGEIIVSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPIL 360
           SLKLPVVLHQPH+G+FM+DNPRFGT I+LPF+LLPS+ KVVGIL++NIYIQSFA PS  L
Sbjct: 301 SLKLPVVLHQPHVGQFMTDNPRFGTTIVLPFQLLPSAGKVVGILQENIYIQSFAGPSTFL 360

Query: 361 VPPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDL 420
           VPP FSLLPPQSTSI P+LA FVGKFS+V+S+G L LNSS +VKKSPIVRFNYYSH DDL
Sbjct: 361 VPPAFSLLPPQSTSINPTLASFVGKFSDVNSKGWLHLNSSLDVKKSPIVRFNYYSHHDDL 420

Query: 421 ARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTT 480
            RCV GVRK+GDLLKTQT+EKIKTQDLEG K F FLGLPL ENL NDSSV+EYC KTVTT
Sbjct: 421 DRCVRGVRKVGDLLKTQTIEKIKTQDLEGEKAFMFLGLPLTENLWNDSSVKEYCKKTVTT 480

Query: 481 YWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           YWHYHGGCLVGKVVDGNYKVIGI+NLRVVDGSTFS SPGTNPMATLMMLGR
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 531

BLAST of HG10006237 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 518.1 bits (1333), Expect = 8.5e-147
Identity = 258/534 (48.31%), Postives = 363/534 (67.98%), Query Frame = 0

Query: 7   TILILSLIISIFQLAVLSSHAIP--NQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCP 66
           ++L  +L++ +    V  S+A P  N+   +M+F+ NA+D  +++ YDYII+GGGTAGCP
Sbjct: 8   SLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCP 67

Query: 67  LATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRG 126
           LA TLS +F VLLLERG  P   P+V++  G L      ++  +P Q F SE+GV N RG
Sbjct: 68  LAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARG 127

Query: 127 RVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRS 186
           RVLGG S +NAGFYSR  +QFFE +G+ WD+  V ++YEWVE  +  RP L  WQ+A R 
Sbjct: 128 RVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRD 187

Query: 187 ALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRI 246
           ALLE GV P NGF L H VGTK GGS FD  G RH + +LL  A+ +NI+VA+ ATV+R+
Sbjct: 188 ALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERV 247

Query: 247 LF--------SGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKS 306
           L         S ++A GV+Y D  G+ H A IR++GE+I+SAGA+GSPQLL LSG+GP+S
Sbjct: 248 LLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRS 307

Query: 307 YLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPS 366
           YLS+  +PV L QPH+G+F+ DNPR G +I+ P  +  S ++VVG+ ED  ++++ ++  
Sbjct: 308 YLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVI 367

Query: 367 PILVPPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHP 426
           P   P     +   ++ +   +   + K     S G LRL +ST+V+ +P+VRFNY+S P
Sbjct: 368 PFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDP 427

Query: 427 DDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKT 486
            DL RCV+G RK+G++L+++ M+    ++  GN+ F F+G PLP +  ND  + ++C +T
Sbjct: 428 QDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRT 487

Query: 487 VTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           V+T WHYHGG +VGKVVD + KVIG+ +LR+VDGSTF+ SPGTNP ATLMMLGR
Sbjct: 488 VSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGR 540

BLAST of HG10006237 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 411.4 bits (1056), Expect = 1.1e-114
Identity = 250/565 (44.25%), Postives = 324/565 (57.35%), Query Frame = 0

Query: 11  LSLIISIF-QLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLATTL 70
           L + +S+F    + SS   PN       F+ +A+  P    YDYIIIGGGTAGCPLA TL
Sbjct: 10  LFVALSLFLHSPICSSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATL 69

Query: 71  SSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDG---KNPFQRFTSEDGVENIRGRV 130
           S N SVLLLERG  P   P++      L+ F A        +P QRF SEDGV N R RV
Sbjct: 70  SQNASVLLLERGDSPYNNPNITR----LSAFGAALSDLSESSPSQRFVSEDGVINARARV 129

Query: 131 LGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSAL 190
           LGGGS +NAGFY+R   ++    G  WD  L  ++Y+WVE  V  +P +  WQ+A R  L
Sbjct: 130 LGGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGL 189

Query: 191 LEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILF 250
           LEAG++P+NGF   H+ GTK GG+IFD  GNRH A +LL  A P  I V + ATV RILF
Sbjct: 190 LEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILF 249

Query: 251 --SGLT---ASGVLYSDSKGKLHRAFIRE--KGEIIVSAGAIGSPQLLLLSGVGPKSYLS 310
              G T   A+GV+Y D  G+ HRA+++E    EII+SAG +GSPQLL+LSGVGP + L 
Sbjct: 250 RTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQ 309

Query: 311 SLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQ--------- 370
           +  + VV+ QPH+G+ M DNP     +  P  +  S ++VVGI  +  Y++         
Sbjct: 310 AQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGG 369

Query: 371 ----SFASPSP----ILVPPIFSLLPPQSTSIIPSLAMFVG-----KFSEVHSEGSLRLN 430
               S  S S      +  P  +LL   S + + S   F G     K     S G L L 
Sbjct: 370 GGGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL- 429

Query: 431 SSTNVKKSPIVRFNYYSHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEF--- 490
            + N K +PIV FNY+ HPDDL RCV G++ +  +++++   + K  D+     FE+   
Sbjct: 430 KTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVS----FEYLLN 489

Query: 491 LGLPLPENLLN---------DSSVEEYCHKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNL 531
           L    P NL             S EE+C  TVTT WHYHGGC+VG+VVDG+YKVIGI  L
Sbjct: 490 LTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRL 549

BLAST of HG10006237 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 392.1 bits (1006), Expect = 7.0e-109
Identity = 237/542 (43.73%), Postives = 313/542 (57.75%), Query Frame = 0

Query: 34  SYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNFSVLLLERGSDP--NKYPSVL 93
           S   F  N  D      YDYI+IGGGTAGCPLA TLS NFSVL+LERG  P  N   S L
Sbjct: 50  SSSSFSSNGQD----SSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFL 109

Query: 94  NEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGV 153
                 ++  A     +  Q F S DGV N R RVLGGGS +NAGFYSR    F + AG 
Sbjct: 110 RN---FHIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG- 169

Query: 154 DWDMELVEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSI 213
            WD +LV+++Y WVE  +  +P L  WQ A R +LLE GV P NGF   H+ GTK GG+I
Sbjct: 170 -WDPKLVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTI 229

Query: 214 FDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILF--SGL--TASGVLYSDSKGKLHRAF 273
           FD  G RH A ELL  A P  ++V I ATVQ+I+F  SG     +GV++ D KG  H+A 
Sbjct: 230 FDRFGRRHTAAELLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQAL 289

Query: 274 I--REKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKLPVVLHQPHIGEFMSDNPRFGTN 333
           +  R+  E+I+S+GAIGSPQ+L+LSG+GPK  L  LK+PVVL   H+G+ M+DNP     
Sbjct: 290 LSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM--NT 349

Query: 334 IILPFK--LLPSSVKVVGILEDNIYIQSFA----SPSPILV--------PPIFSLLP--- 393
           I++P K  +  S ++ VGI +  +Y+++      SP  I            +FS +P   
Sbjct: 350 ILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQ 409

Query: 394 --PQSTSIIPSL-----------AMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSH 453
             P++T    +            +  + K +   S G L L  +TNV  +P V FNY+ H
Sbjct: 410 RRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKH 469

Query: 454 PDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENL------LNDS-S 513
           P DL RCV  +R +  ++ +     +     +   V + L L +  N+      LND+ S
Sbjct: 470 PVDLQRCVEAIRLVSKVVTSNRF--LNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKS 529

Query: 514 VEEYCHKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMML 531
           + ++C  TV T WHYHGGCLVGKVV  N KV+G+  LRV+DGSTF ESPGTNP AT+MM+
Sbjct: 530 MAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMM 577

BLAST of HG10006237 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 384.0 bits (985), Expect = 1.9e-106
Identity = 224/527 (42.50%), Postives = 309/527 (58.63%), Query Frame = 0

Query: 38  FVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLL 97
           F+ +A+  P    +DYIIIGGGTAGC LA TLS N SVL+LERG  P + P+  +    +
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSV 119

Query: 98  NVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMEL 157
           N     +   +  Q F SEDGV N R RVLGGGS++N GFYSR    + E A  +W+ME 
Sbjct: 120 NTL-LNNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEA--EWEMEE 179

Query: 158 VEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGN 217
           VE AYEWVE+ +   P +  WQ AF+  LLEAG  PDNGF   H+ GTK GG+IFD  G+
Sbjct: 180 VEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGH 239

Query: 218 RHGAVELLNKAKPTNIKVAIEATVQRILFSGLTASGVLYSDSKGKLHRAFIREK--GEII 277
           RH A  LL  A P  I V + A+V ++LF+   A  VL+ D+ G  H+A +  K   E+I
Sbjct: 240 RHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGVFHKANLANKATNEVI 299

Query: 278 VSAGAIGSPQLLLLSGVGPKSYLSSLKL-PVVLHQPHIGEFMSDNPRFGTNIILPFKLLP 337
           +SAGA+GSPQLL+LSGVGP  +L +  + P+VL QP +G+ M+DNP     I  P  +  
Sbjct: 300 LSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQPVEL 359

Query: 338 SSVKVVGILEDNIYIQSFASPS------PILVPPIFSLLPPQSTS-----IIPSLAMFVG 397
           S ++ VGI + + YI+  +  S            + +LL   S +     +  S+A+ + 
Sbjct: 360 SLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLLK 419

Query: 398 KFS---EVHSEGSLRLNS-------------STNVKKSPIVRFNYYSHPDDLARCVSGVR 457
            F    EV   G L                 +TN + +P V FNYY  P+DL +CV G+ 
Sbjct: 420 SFDVKLEVRMNGGLIFQKVDGPASKGHMKLRNTNPRDNPSVTFNYYQEPEDLNKCVKGLN 479

Query: 458 KMGDLLKTQTMEKIKTQDLEGNKVFE-FLGLPL---PENLLNDSSVEEYCHKTVTTYWHY 517
            +  ++ ++   K K   +   ++    L LP+   P ++ +  +++++C  TVT+ WHY
Sbjct: 480 TIIRMINSKAFSKYKYPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVWHY 539

Query: 518 HGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           HGGC VGKVVD NYKV+GI  LRV+DGSTF +SPGTNP AT+MMLGR
Sbjct: 540 HGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLGR 582

BLAST of HG10006237 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.8e-104
Identity = 228/530 (43.02%), Postives = 305/530 (57.55%), Query Frame = 0

Query: 37  KFVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGL 96
           +F+ +A+  P    +DYIIIGGGTAGC LA TLS N +VL+LERG  P   P+  +    
Sbjct: 32  RFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQNATVLVLERGGSPYDDPAATDIGNF 91

Query: 97  LNVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDME 156
            N         +  Q F SEDGV N R RVLGGG+++NAGFYSR    F   AG  W+ +
Sbjct: 92  ANTL-LNITPNSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG--WERD 151

Query: 157 LVEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKG 216
            VE AYEWVE+ V   P +N WQSAFR  LLEAGV P NGF   H+VGTK GG+IFD  G
Sbjct: 152 EVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDG 211

Query: 217 NRHGAVELLNKAKPTNIKVAIEATVQRILFS-----GLTASGVLYSDSKGKLHRAFIREK 276
           +RH A  LL  A P  I V + A+V +ILF+        A GV++ D+ G  ++A +  +
Sbjct: 212 HRHTAANLLEYANPNMIVVYLHASVHKILFTIKGNQRPKAYGVIFLDANGVSYKAELATQ 271

Query: 277 ----GEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKL-PVVLHQPHIGEFMSDNPRFGTNI 336
                E+I+SAGAI SPQLL+LSGVGP ++L++ ++ PV++ QP +G+ M DNP     I
Sbjct: 272 DSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFI 331

Query: 337 ILPFKLLPSSVKVVGILEDNIYIQ--SFASPSPILVPPIF---------SLLPPQSTS-- 396
             P  +  S V+ VGI +   YI+  S  S S  L    F         + LP QS S  
Sbjct: 332 PSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQSISKF 391

Query: 397 ---------IIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLARCVS 456
                    +     + + K +   S G L L  +TN   +P V FNY+  P+DL +CV 
Sbjct: 392 FKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELR-NTNPDDNPSVTFNYFKDPEDLNKCVE 451

Query: 457 GVRKMGDLLKTQTMEKIKTQDLEGNKVFE-FLGLPL---PENLLNDSSVEEYCHKTVTTY 516
           G+  +  ++ ++   K K        +    L LP    P ++ +   +E+YC  TV T 
Sbjct: 452 GLSTIIKVIDSKGYSKYKYPLASARGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMTI 511

Query: 517 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 531
           +HYHGGC VGKVVD NYKV+G+  LR++DGSTF +SPGTNP AT+MMLGR
Sbjct: 512 YHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTFLKSPGTNPQATIMMLGR 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038889314.11.0e-27991.13(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_011650116.24.3e-27890.19(R)-mandelonitrile lyase 1 [Cucumis sativus][more]
XP_038889315.18.2e-27789.43(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
KAE8651660.13.6e-27290.14hypothetical protein Csa_021193 [Cucumis sativus][more]
XP_008452041.16.9e-26887.36PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
P527064.8e-15551.89(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K21.3e-15250.28(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527073.0e-14949.72(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O827841.2e-14849.72(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
O242434.1e-14648.88(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LK768.0e-27890.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1[more]
A0A5A7VNU13.4e-26887.36(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BSB53.4e-26887.36(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493164 PE=3 SV=... [more]
A0A5A7VGR61.1e-26385.47(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BTS58.3e-25984.56(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G73050.18.5e-14748.31Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.11.1e-11444.25Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.17.0e-10943.73Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51930.11.9e-10642.50Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.11.8e-10443.02Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 364..530
e-value: 4.3E-29
score: 99.7
coord: 31..337
e-value: 1.1E-54
score: 184.1
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 51..319
e-value: 1.2E-31
score: 110.2
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 279..293
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 124..147
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 389..529
e-value: 1.3E-28
score: 100.3
NoneNo IPR availableGENE3D3.30.410.40coord: 171..479
e-value: 2.6E-169
score: 566.2
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 7..530
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 7..530
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 389..486
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 51..529
e-value: 2.6E-169
score: 566.2
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 31..530

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10006237.1HG10006237.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors