HG10006231 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10006231
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	(R)-mandelonitrile lyase 1-like
Location	Chr07: 16230248 .. 16232496 (+)
RNA-Seq Expression	HG10006231
Synteny	HG10006231

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCATTTTGTGGCTATCGTTCTCCTCATCTTTTCCTCTGTGTTATTTCACTTCCAATTGGGAGTCCTCTCCCCAAACTCCATCCCCAATCAAGGTAAAAACTTTTCGTTATGTCCAAAATTTAATCAGTCCATCCAAGAATAGTTTTAGATTATTATATATTATGAGCTTCTACTTTTTTAATATATATTTCAGATGTTAGCTACATGAAATTTGTAGAAGATGCCAATGAATTACCAGAAAGTGAAGAATATGACTACATAATAATAGGAGGAGGAACAGCTGGCTGTCCATTAGCTGCAACGTTATCATCAAATTTCTCAGTTCCTCTTTTGGAAAGAGGCAGTGATCCTAATCCATATCCATCTCTACTTAATGAACAAGGTTTGTTGAACGTTTTTTCTACTGAAGATGATGGCAAAAATCCCTTCCAACGATTTATCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGGGTCCTTGGTGGCGGGAGTATGGTTAATGCTGGCTTTTACTCGAGGGGTCATCGAGAGTTCTTTGAAACAGCAGGTGTTGATTGGGATATGGAATTGGTAGAGAAGGCTTATGAATGGGGTGAAGAAACTGTGGTATCTCAACCAAGTTTGAATAATGCTTGGCAAGCTGCTTTTAGAAATGTTTTGTTGGAAGCTGGGATTCAACCTGATAATGGGTTTGATTTGAGGAACCTTGTGGGGACTAGAATTGGAGGATCCATCTTTGATAATAAAGGAAACAGACATGGAGCTGTAGAGCTTCTCAATAAAGCTGAATTCAAAAACCTCAAAGTTGTAATTCAAGCCATAGTCCAAAGAATCATCTTCTCTGGTAATCAATGTTAACTAAAAATAAAGGGAAAATTACCATTTTAGTCCCCAAGTTTTGAACAATATGTGCGTTTAGTCCCTAAGTTTTAAAAATGTACCTTTTTAGTCCCTTAGTTTATAAGAATAGGTCTATTTGGTCCCTGAGTTTTCAAAATATACCTTTTCAGTTCCTGAGTTTTTAAAAATAGGTCTAAGAGATCCCTCAAATAATTTTTATTATAATTAAATAATATATGACATTTTAAATTATAAGAATAATTTTAAAAACCATTTCGTCTCTAAAACTATTTTTCTAATTTTAAATATCATATAATTATTTTTAAAAAATTAAAGAAAAAATATTTGGAGGACCTTTTAGACCTATTTTTAAAAACTCAGGGACTGAAAGGTTTTTTTGAAAACTCAGGGACCAAATTGATCTATTCTTATAAACTGAGGGACTAAAAAGGTACATTTTTAAAAATTAGGGACCAAACACACATATTGTTGAAAACTTGAGAACTAAAAAGGTAATTTTCTCAAAAATAAATTTTATTCCAGCCATAGACTTCTATCGGCGTCTATCACAACTATCTACAAATTTTTCTATTTGGTGTAATAGTTTTCCTTATCTTCTTATTTTTAAAAATCACCCTTGAAAAACATTGAATTTTCTAATATTATCTTTGTAGGTTTATCTGCAAGTGGGGTGTCATATTCTGATTCAAAAGGAAAGTTGCATAAAGCATTTATTCGTGAGAAAGGAGAGATAATTGTAAGTGCTGGAGCCATTGGAAGTCCTCAGCTTCTCCTTCTAAGTGGGGTTGGCCCAAAAGCTCATCTTTCATCCTTAAAACTACATGTCGTTCTTGACCAACCACATGTCGGTGAATTCATGGCTGACAATCCTCGTTTTGGTGTCAACCTTATGCTTCCATTCCAACTAGTTGCTTCATCTGCAAAAGTGGTTGGAACTATAGAAAACAATATCCATTTCCAAACAATTGCAAGTATTTTTCAATTTTCACTTCCACCATCATTTAGTCTTTTTCCTTCTAATCAACATGGCTTAGTGAATTTAAGCTTAGCCTTAATTCTTGGAAAATTTTCTGAGGTGTTTTCTGTGGGATCGTTAAGGTTGAATTCTTCTATAAGACGCGGGATTTGGAGGGTAAGAAAGGATTTCAATTTCTTGGGCCTCCGTTGCCGGAAAATTTGTCGGATGATAATTCAGTTGGAGAATTCTGTCGGAATGTAGTGGCAACATATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTTGTCGACGGTAATTATAAAGTCATCGGAATTGAAAATCTGCGTGTAGTTGATGGCTCCACTTTCTCCGATTCACCTGGAACCAATCCTATGGCCACCCTTATGATGCTCGGACGGTGA

mRNA sequence

ATGGAGCATTTTGTGGCTATCGTTCTCCTCATCTTTTCCTCTGTGTTATTTCACTTCCAATTGGGAGTCCTCTCCCCAAACTCCATCCCCAATCAAGATGTTAGCTACATGAAATTTGTAGAAGATGCCAATGAATTACCAGAAAGTGAAGAATATGACTACATAATAATAGGAGGAGGAACAGCTGGCTGTCCATTAGCTGCAACGTTATCATCAAATTTCTCAGTTCCTCTTTTGGAAAGAGGCAGTGATCCTAATCCATATCCATCTCTACTTAATGAACAAGGTTTGTTGAACGTTTTTTCTACTGAAGATGATGGCAAAAATCCCTTCCAACGATTTATCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGGGTCCTTGGTGGCGGGAGTATGGTTAATGCTGGCTTTTACTCGAGGGGTCATCGAGAGTTCTTTGAAACAGCAGGTGTTGATTGGGATATGGAATTGGTAGAGAAGGCTTATGAATGGGGTGAAGAAACTGTGGTATCTCAACCAAGTTTGAATAATGCTTGGCAAGCTGCTTTTAGAAATGTTTTGTTGGAAGCTGGGATTCAACCTGATAATGGGTTTGATTTGAGGAACCTTGTGGGGACTAGAATTGGAGGATCCATCTTTGATAATAAAGGAAACAGACATGGAGCTGTAGAGCTTCTCAATAAAGCTGAATTCAAAAACCTCAAAGTTGTAATTCAAGCCATAGTCCAAAGAATCATCTTCTCTGGAAAGTTGCATAAAGCATTTATTCGTGAGAAAGGAGAGATAATTGTAAGTGCTGGAGCCATTGGAAGTCCTCAGCTTCTCCTTCTAAGTGGGGTTGGCCCAAAAGCTCATCTTTCATCCTTAAAACTACATGTCGTTCTTGACCAACCACATGTCGGTGAATTCATGGCTGACAATCCTCGTTTTGGTGTCAACCTTATGCTTCCATTCCAACTAGTTGCTTCATCTGCAAAAGTGGTTGGAACTATAGAAAACAATATCCATTTCCAAACAATTGCAAGTGTTTTCTGTGGGATCGTTAAGGTTGAATTCTTCTATAAGACGCGGGATTTGGAGGGTAAGAAAGGATTTCAATTTCTTGGGCCTCCGTTGCCGGAAAATTTGTCGGATGATAATTCAGTTGGAGAATTCTGTCGGAATGTAGTGGCAACATATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTTGTCGACGGTAATTATAAAGTCATCGGAATTGAAAATCTGCGTGTAGTTGATGGCTCCACTTTCTCCGATTCACCTGGAACCAATCCTATGGCCACCCTTATGATGCTCGGACGGTGA

Coding sequence (CDS)

ATGGAGCATTTTGTGGCTATCGTTCTCCTCATCTTTTCCTCTGTGTTATTTCACTTCCAATTGGGAGTCCTCTCCCCAAACTCCATCCCCAATCAAGATGTTAGCTACATGAAATTTGTAGAAGATGCCAATGAATTACCAGAAAGTGAAGAATATGACTACATAATAATAGGAGGAGGAACAGCTGGCTGTCCATTAGCTGCAACGTTATCATCAAATTTCTCAGTTCCTCTTTTGGAAAGAGGCAGTGATCCTAATCCATATCCATCTCTACTTAATGAACAAGGTTTGTTGAACGTTTTTTCTACTGAAGATGATGGCAAAAATCCCTTCCAACGATTTATCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGGGTCCTTGGTGGCGGGAGTATGGTTAATGCTGGCTTTTACTCGAGGGGTCATCGAGAGTTCTTTGAAACAGCAGGTGTTGATTGGGATATGGAATTGGTAGAGAAGGCTTATGAATGGGGTGAAGAAACTGTGGTATCTCAACCAAGTTTGAATAATGCTTGGCAAGCTGCTTTTAGAAATGTTTTGTTGGAAGCTGGGATTCAACCTGATAATGGGTTTGATTTGAGGAACCTTGTGGGGACTAGAATTGGAGGATCCATCTTTGATAATAAAGGAAACAGACATGGAGCTGTAGAGCTTCTCAATAAAGCTGAATTCAAAAACCTCAAAGTTGTAATTCAAGCCATAGTCCAAAGAATCATCTTCTCTGGAAAGTTGCATAAAGCATTTATTCGTGAGAAAGGAGAGATAATTGTAAGTGCTGGAGCCATTGGAAGTCCTCAGCTTCTCCTTCTAAGTGGGGTTGGCCCAAAAGCTCATCTTTCATCCTTAAAACTACATGTCGTTCTTGACCAACCACATGTCGGTGAATTCATGGCTGACAATCCTCGTTTTGGTGTCAACCTTATGCTTCCATTCCAACTAGTTGCTTCATCTGCAAAAGTGGTTGGAACTATAGAAAACAATATCCATTTCCAAACAATTGCAAGTGTTTTCTGTGGGATCGTTAAGGTTGAATTCTTCTATAAGACGCGGGATTTGGAGGGTAAGAAAGGATTTCAATTTCTTGGGCCTCCGTTGCCGGAAAATTTGTCGGATGATAATTCAGTTGGAGAATTCTGTCGGAATGTAGTGGCAACATATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTTGTCGACGGTAATTATAAAGTCATCGGAATTGAAAATCTGCGTGTAGTTGATGGCTCCACTTTCTCCGATTCACCTGGAACCAATCCTATGGCCACCCTTATGATGCTCGGACGGTGA

Protein sequence

MEHFVAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGGTAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAIVQRIIFSGKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAHLSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIASVFCGIVKVEFFYKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR

Homology

[Close]

BLAST of HG10006231 vs. NCBI nr
Match: XP_038889314.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 649.8 bits (1675), Expect = 1.7e-182
Identity = 346/532 (65.04%), Postives = 393/532 (73.87%), Query Frame = 0


Query: 1   MEHFVAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGG 60
           MEH  A  +LI S ++   QLGVL+ N+IP +DV YMKFV  A++LP  EEYDYIIIGGG
Sbjct: 1   MEHSKA-TILILSLMISISQLGVLTYNAIPIKDVRYMKFVHKASDLPAKEEYDYIIIGGG 60

Query: 61  TAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGV 120
           TAGCPLAATLSS FSV +LERGSDPN YPS+LNEQGLLNVF+  DDGKNPFQRFISEDGV
Sbjct: 61  TAGCPLAATLSSKFSVLVLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFISEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAW 180
           ENIR RVLGGGSMVNAGFYSRGH++FFETAGVDWDMELVEKAY+W EETV S+P L NAW
Sbjct: 121 ENIRARVLGGGSMVNAGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVASRPIL-NAW 180

Query: 181 QAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQ 240
           Q+AFR+ LLEAG+ PDNGFDLR+LVGT+ GGSIFD+KGNRHGAVELLNKAE  N+KV I+
Sbjct: 181 QSAFRSALLEAGVVPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPTNIKVAIE 240

Query: 241 AIVQRIIFS-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAHL 300
           A VQ+I+FS             GKLH+AFIR+KGEIIVSAGAIGSPQLLLLSGVGPK+HL
Sbjct: 241 ATVQKILFSGLSARGVLYSDSKGKLHRAFIRKKGEIIVSAGAIGSPQLLLLSGVGPKSHL 300

Query: 301 SSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS---- 360
           SSLKL VVL QP+VGEFM+DNPRFG N++LPFQLV SS KVVG +++NI+ Q+ AS    
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPF 360

Query: 361 ---------------------VFCG-----------------------IVKVEFF----- 420
                                +F G                       IV+  ++     
Sbjct: 361 FAPPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDD 420

Query: 421 ----------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVA 445
                                  KT DLEG KGFQFLG PLPENLS+D+SVGE+C+  V 
Sbjct: 421 LARCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVGEYCQKTVT 480

[Close]

BLAST of HG10006231 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 647.9 bits (1670), Expect = 6.4e-182
Identity = 343/532 (64.47%), Postives = 389/532 (73.12%), Query Frame = 0


Query: 1   MEHFVAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGG 60
           MEH  +  +LI S ++  F LG+LS ++IPNQDV YMKFV  A +LP  EEYDYIIIGGG
Sbjct: 1   MEHSRS-TILILSLMISIFHLGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGGG 60

Query: 61  TAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGV 120
           TAGCPL++TLSS FSV LLERGSDPN YPS+LNEQGLLN F+ EDDGKNPFQ FISEDGV
Sbjct: 61  TAGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAW 180
           ENIRGRVLGGGSMVN GFYSRGH++FFETAGVDWDMELVEKAY+W EETVVSQP L NAW
Sbjct: 121 ENIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPIL-NAW 180

Query: 181 QAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQ 240
           Q+AFR+  L+AG+ PDNGFDLR+ VGT+IGGSIFDNKGNRHGAVELLNKA   N+KV I+
Sbjct: 181 QSAFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIE 240

Query: 241 AIVQRIIFS-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAHL 300
           A VQRI+FS             GKLH+AFIR++GEIIVSAGA+GSPQLLLLSGVGPK+HL
Sbjct: 241 ATVQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHL 300

Query: 301 SSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS---- 360
           SSLKL VVL QP+VGEFM+DNPRFG N++LPFQLV SS KVVG +++NI+ Q+ AS    
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPF 360

Query: 361 ---------------------VFCG-----------------------IVKVEFF----- 420
                                +F G                       IV+  ++     
Sbjct: 361 FAPPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDD 420

Query: 421 ----------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVA 445
                                  KT DLEG KGFQFLG PLPENLS+D+SV E+C+  V 
Sbjct: 421 LARCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVT 480

[Close]

BLAST of HG10006231 vs. NCBI nr
Match: XP_038889699.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-181
Identity = 346/532 (65.04%), Postives = 391/532 (73.50%), Query Frame = 0


Query: 1   MEHFVAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGG 60
           M+  +A +LLI   ++  FQLGV S ++IP+QD SY+KFV +ANELPE+ EYDYIIIGGG
Sbjct: 1   MKRSMATILLII--LISIFQLGVFSSHTIPDQDTSYLKFVHEANELPETGEYDYIIIGGG 60

Query: 61  TAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGV 120
           TAGCPLAATLSS +SV LLERGSDPN YPS+LNEQGLLN F  EDDG+NPFQ FISEDGV
Sbjct: 61  TAGCPLAATLSSKYSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAW 180
           ENIRGRVLGGGSM+NAGFYSRGHREFFETAGVDWDMELVEKAY+W E+TVVS+PSL +AW
Sbjct: 121 ENIRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEQTVVSRPSL-SAW 180

Query: 181 QAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQ 240
           Q+AFR  LLEAG+ PDNGFDL++LVGT+ GGSIFDNKGNRHGAVELLNKAE +NLKV  Q
Sbjct: 181 QSAFRGALLEAGVGPDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVGTQ 240

Query: 241 AIVQRIIFS-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAHL 300
           AIV+RIIF+             GKLH AFIREKGEII+SAGAIGSPQLLLLSGVGPK HL
Sbjct: 241 AIVERIIFTGLSASGVSYSDPKGKLHTAFIREKGEIILSAGAIGSPQLLLLSGVGPKYHL 300

Query: 301 SSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS---- 360
           SSLKL VVL QPHVGEFM+DNPRF   ++LPFQLVASSA+VVGT+ NNIH Q+ AS    
Sbjct: 301 SSLKLPVVLHQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLANNIHLQSFASPLPF 360

Query: 361 ---------------------VFCG-----------------------IVKVEFF----- 420
                                +F G                       IV+  ++     
Sbjct: 361 FAPPSFSLLPPQSTSIIPSLAIFVGKFSDVLSEGSLRLNSSIDVKESPIVRFNYYSNHDD 420

Query: 421 ----------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVA 445
                                  K +DLEG KGFQFLG PLPENL +D++V E+C+  V 
Sbjct: 421 LARCVRGVRKVGDLLKTQTMEKIKNQDLEGNKGFQFLGLPLPENLWNDSAVEEYCQKTVT 480

[Close]

BLAST of HG10006231 vs. NCBI nr
Match: XP_022153314.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 643.3 bits (1658), Expect = 1.6e-180
Identity = 341/533 (63.98%), Postives = 389/533 (72.98%), Query Frame = 0


Query: 1   MEHF-VAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGG 60
           M+H+ +AI LL+F   +FHFQL VLS ++IPNQDVSYMKFVEDA +LPE EEYDYII+GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDG 120
           GTAGCPLA TLSS FSV LLERGS+PN +PS+L E+GLLN F+ EDD KNPFQRFISEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNA 180
           VEN+RGRVLGG SM+NAGFYSRGHRE+FE AGV+WDMELVEKAY+W EETVVS+PSL +A
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHREYFEKAGVEWDMELVEKAYQWVEETVVSRPSL-SA 180

Query: 181 WQAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVI 240
            Q  FR+ LLE+G+ PDNGF LR+LVGTRIGGSIFD KGNRHGAVELLNKA   NLKV I
Sbjct: 181 LQPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNRHGAVELLNKANPTNLKVAI 240

Query: 241 QAIVQRIIFS-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAH 300
           +A V+RIIFS             GKLH AFIR KGEII+SAGAIGSPQLLLLSG+GPK+ 
Sbjct: 241 RATVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQ 300

Query: 301 LSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS--- 360
           LSSLKL VVL QP+VGEFM+DNPRFG +++LPF  V +SAKVVGT+ENNIH QT+AS   
Sbjct: 301 LSSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPFVPTSAKVVGTLENNIHLQTVASPLP 360

Query: 361 ----------------------VFCG-----------------------IVKVEFF---- 420
                                 +F G                       IV+  ++    
Sbjct: 361 FSIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPD 420

Query: 421 -----------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVV 445
                                   KTRD EGKK FQF+GP LPENLSDD++VGEFC   +
Sbjct: 421 DLARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSL 480

[Close]

BLAST of HG10006231 vs. NCBI nr
Match: XP_022153315.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 637.5 bits (1643), Expect = 8.6e-179
Identity = 338/533 (63.41%), Postives = 387/533 (72.61%), Query Frame = 0


Query: 1   MEHF-VAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGG 60
           M+H+ +AI LL+F   +FHFQL VLS ++IPNQDVSYMKFVEDA +LPE EEYDYII+GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDG 120
           GTAGCPLA TLSS FSV LLERGS+PN +PS+L E+GLLN F+ EDD KNPFQRFISEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNA 180
           VEN+RGRVLGG SM+NAGFYSRGH+EFFE AGV+WDMELVEKAY+W EETVVS+PSL +A
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHQEFFEKAGVEWDMELVEKAYQWVEETVVSRPSL-SA 180

Query: 181 WQAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVI 240
            Q  FR+ LLE+G+ PDNGF LR+LVGTRIGGSIFD KGN HGAVELLNKA   N+KV I
Sbjct: 181 LQPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNPHGAVELLNKANPTNIKVAI 240

Query: 241 QAIVQRIIFS-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAH 300
           +  V+RIIFS             GKLH AFIR KGEII+SAGAIGSPQLLLLSG+GPK+ 
Sbjct: 241 RTTVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQ 300

Query: 301 LSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS--- 360
           LSSLKL VVL QP+VGEFM+DNPRFG +++LPF  V +SAKVVGT+ENNIH QT+AS   
Sbjct: 301 LSSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPXVPTSAKVVGTLENNIHLQTVASPLP 360

Query: 361 ----------------------VFCG-----------------------IVKVEFF---- 420
                                 +F G                       IV+  ++    
Sbjct: 361 FSIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPD 420

Query: 421 -----------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVV 445
                                   KTRD EGKK FQF+GP LPENLSDD++VGEFC   +
Sbjct: 421 DLARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSL 480

[Close]

BLAST of HG10006231 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 3.1e-115
Identity = 229/531 (43.13%), Postives = 303/531 (57.06%), Query Frame = 0


Query: 5   VAIVLLIFSSVLFHFQLG-VLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGGTAG 64
           ++ VLL+    + H Q   V S  +  + D SY+ FV DA +      YDYII+GGGTAG
Sbjct: 6   MSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAG 65

Query: 65  CPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVENI 124
           CPLAATLS+N+SV +LERGS P  YP+LL   G +     EDDGK P +RF+SEDG++N+
Sbjct: 66  CPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 125 RGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQAA 184
           RGRVLGG SM+NAG Y R +  FF   G++WDM+LV + YEW E+T+V +P  +  WQ  
Sbjct: 126 RGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPD-SQTWQTV 185

Query: 185 FRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAIV 244
                LEAGI P+NGF + +L GTR+ GS FDN G RH + ELLNK +  NL+V +QA V
Sbjct: 186 IGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAV 245

Query: 245 QRIIFS-----------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAH 304
           ++IIFS                 G  H+AF+R +GE+I+SAG IGSPQLLLLSGVGP+++
Sbjct: 246 EKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESY 305

Query: 305 LSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVG---------------- 364
           L+SL + VV   P+VG+++ DNPR  +N++ P  + AS+  V+G                
Sbjct: 306 LTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSDFYQCSISSLPFD 365

Query: 365 --------TIENNIHFQTIASVFCGI----------------------VKVEFF------ 424
                   T    +  QT A +   +                      VK  ++      
Sbjct: 366 TPPFSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDL 425

Query: 425 ---------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVAT 445
                                YK  DL G  GF  LG PLPEN +DD +   FCR  VA+
Sbjct: 426 SHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVAS 485

[Close]

BLAST of HG10006231 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.0e-114
Identity = 227/529 (42.91%), Postives = 305/529 (57.66%), Query Frame = 0


Query: 5   VAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGGTAGC 64
           + +VL +F  +L + ++  L+  S  N D SY++F  DA +L     YDY+I+GGGT+GC
Sbjct: 9   ILLVLHLFVLLLQYSEVHSLATTS--NHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGC 68

Query: 65  PLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVENIR 124
           PLAATLS  + V +LERGS P  YP++L   G +     EDDGK P +RF+SEDG++N+R
Sbjct: 69  PLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVR 128

Query: 125 GRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQAAF 184
           GRVLGG SM+NAG Y+R +   +  +GVDWDM+LV K YEW E+T+V +P+    WQ+  
Sbjct: 129 GRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNY-QPWQSVT 188

Query: 185 RNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAIVQ 244
               LEAG+ P++GF L +  GTRI GS FDNKG RH A ELLNK    NL+V + A V+
Sbjct: 189 GTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVE 248

Query: 245 RIIFS----------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAHLS 304
           +IIFS                G  H+AF+R KGE+IVSAG IG+PQLLLLSGVGP+++LS
Sbjct: 249 KIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLS 308

Query: 305 SLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIEN-------------- 364
           SL + VVL  P+VG+F+ DNPR  +N++ P  +  +   V+G   +              
Sbjct: 309 SLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISNDFYQCSFSSLPFTTP 368

Query: 365 -------------NIHFQTIASVFCG-------------------IVKVEFF-------- 424
                        N  F   AS   G                    VK  ++        
Sbjct: 369 PFSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSH 428

Query: 425 -------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVATYW 445
                              YK  DL G +GF  LG PLP++ +DD +   FCR  VA+YW
Sbjct: 429 CVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYW 488

[Close]

BLAST of HG10006231 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.2e-113
Identity = 225/528 (42.61%), Postives = 304/528 (57.58%), Query Frame = 0


Query: 6   AIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGGTAGCP 65
           AI+L+++  VL      V S  +  + D SY+ F  DA +L     YDY+I+GGGT+GCP
Sbjct: 8   AILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSGCP 67

Query: 66  LAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVENIRG 125
           LAATLS  + V +LERGS P  YP++L   G +     EDDGK P +RF+SEDG++N+RG
Sbjct: 68  LAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRG 127

Query: 126 RVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQAAFR 185
           RVLGG S++NAG Y+R +   +  +GVDWDM+LV + YEW E+T+V +P+ + +WQ+  +
Sbjct: 128 RVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPN-SQSWQSVTK 187

Query: 186 NVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAIVQR 245
              LEAG+ P++GF L +  GTRI GS FDNKG RH A ELLNK    NL+V + A V++
Sbjct: 188 TAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEK 247

Query: 246 IIFS----------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAHLSS 305
           IIFS                G  H+AF+R KGE+IVSAG IG+PQLLLLSGVGP+++LSS
Sbjct: 248 IIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSS 307

Query: 306 LKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIEN--------------- 365
           L + VVL  P+VG+F+ DNPR  +N++ P  +  +   V+G   +               
Sbjct: 308 LNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISNDFYQCSFSSLPFTTPP 367

Query: 366 ------------NIHFQTIASVFCG-------------------IVKVEFF--------- 425
                       N  F   AS   G                    VK  ++         
Sbjct: 368 FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHC 427

Query: 426 ------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVATYWH 445
                             YK  DL G +GF  LG PLP++ +DD +   FCR  VA+YWH
Sbjct: 428 VSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVASYWH 487

[Close]

BLAST of HG10006231 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.5e-112
Identity = 225/530 (42.45%), Postives = 299/530 (56.42%), Query Frame = 0


Query: 6   AIVLLIFSSVLFHFQLG-VLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGGTAGC 65
           AI++L     + H Q   V S  +    D SY++FV DAN+      YDYII+GGGTAGC
Sbjct: 8   AILVLALHLFVLHLQYSEVQSLANTSAHDFSYLEFVYDANDTELEGTYDYIIVGGGTAGC 67

Query: 66  PLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVENIR 125
           PLAATLS+N+SV +LERG+ P  YP+LL   G +     EDDG+ P +RF+S DG++N+R
Sbjct: 68  PLAATLSANYSVLVLERGTLPTEYPNLLTSDGFIYNLQQEDDGQTPVERFVSGDGIDNVR 127

Query: 126 GRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQAAF 185
           GRVLGG SM+NAG Y R +  FF   G++WDM+LV K Y+W E+T+V +P     WQ   
Sbjct: 128 GRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNKTYDWVEDTIVFKPDF-QFWQNLT 187

Query: 186 RNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAIVQ 245
               LE GI PDNGF L +L GTR+ GS FDN G RH + ELLNK +  NL+V + A V+
Sbjct: 188 GTAFLEVGILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVHAAVE 247

Query: 246 RIIFS-----------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAHL 305
           +IIFS                 G  H+AF+R  GE+I+SAG IGSPQLLLLSGVG +++L
Sbjct: 248 KIIFSSDSSGVTAIGVIYTDSNGTTHQAFVRGDGEVILSAGPIGSPQLLLLSGVGLESYL 307

Query: 306 SSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVG----------------- 365
           +SL + VV   P+VG+++ DNPR  +N++ P  + AS+  V+G                 
Sbjct: 308 TSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSDFYQCSISSLPFST 367

Query: 366 ------------------------------------------TIENNIHFQ------TIA 425
                                                      +  N+ F        +A
Sbjct: 368 APFGFFPNPTYPLPNTTFAHIVNKVPGPLSHGTVLLQSTSDVRVAPNVTFNYYSNTTDLA 427

Query: 426 SVFCGIVKVEFF--------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVATY 445
               G+ K+  F        YK  DL G +GF  LG PLPEN +DD +   FCR  VA+Y
Sbjct: 428 HCVSGMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIPLPENQTDDAAFETFCREAVASY 487

[Close]

BLAST of HG10006231 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.6e-111
Identity = 218/532 (40.98%), Postives = 307/532 (57.71%), Query Frame = 0


Query: 5   VAIVLLIFSSVLFHFQLGVLS--PNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGGTA 64
           ++ V+L+ + ++ H Q   +    N+    D  Y+KFV +A +L     YDYII+GGGT+
Sbjct: 6   MSAVVLVLNLLVLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIVGGGTS 65

Query: 65  GCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVEN 124
           GCPLAATLS+N+SV +LERG+    YP+ L   G       +DDGK P +RF+SEDG++N
Sbjct: 66  GCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSEDGIDN 125

Query: 125 IRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQA 184
           +R R+LGG +++NAG Y+R +  F+  +GV+WD++LV +AYEW E+ +V +PS N +WQ+
Sbjct: 126 VRSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPS-NQSWQS 185

Query: 185 AFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAI 244
                 LEAG+ PDNGF L +  GTR+ GS FDN G RH + ELLNK +  NLKV ++A 
Sbjct: 186 ITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVAVEAA 245

Query: 245 VQRIIFS-----------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKA 304
           VQ+IIFS                 G  H+A +  KGE+I+SAG +G+PQLLLLSGVGP++
Sbjct: 246 VQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGVGPES 305

Query: 305 HLSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVG--------------- 364
           +L+SL + VV   P+VG+++ DNPR  +N++ P  +  S+  V+G               
Sbjct: 306 YLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSDFYQCSLSSLPF 365

Query: 365 ---------TIENNIHFQTIASV------------------------------FC----- 424
                    T    +  QT A +                              +C     
Sbjct: 366 DTPPFSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFNYCSDPVD 425

Query: 425 ------GIVKVEFF--------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVA 445
                 G+ K+  F        YK  DL G  GF  LG PLPEN +DD +  +FCR+ VA
Sbjct: 426 LTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEKFCRDTVA 485

[Close]

BLAST of HG10006231 vs. ExPASy TrEMBL
Match: A0A6J1DIQ0 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020835 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 7.6e-181
Identity = 341/533 (63.98%), Postives = 389/533 (72.98%), Query Frame = 0


Query: 1   MEHF-VAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGG 60
           M+H+ +AI LL+F   +FHFQL VLS ++IPNQDVSYMKFVEDA +LPE EEYDYII+GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDG 120
           GTAGCPLA TLSS FSV LLERGS+PN +PS+L E+GLLN F+ EDD KNPFQRFISEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNA 180
           VEN+RGRVLGG SM+NAGFYSRGHRE+FE AGV+WDMELVEKAY+W EETVVS+PSL +A
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHREYFEKAGVEWDMELVEKAYQWVEETVVSRPSL-SA 180

Query: 181 WQAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVI 240
            Q  FR+ LLE+G+ PDNGF LR+LVGTRIGGSIFD KGNRHGAVELLNKA   NLKV I
Sbjct: 181 LQPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNRHGAVELLNKANPTNLKVAI 240

Query: 241 QAIVQRIIFS-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAH 300
           +A V+RIIFS             GKLH AFIR KGEII+SAGAIGSPQLLLLSG+GPK+ 
Sbjct: 241 RATVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQ 300

Query: 301 LSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS--- 360
           LSSLKL VVL QP+VGEFM+DNPRFG +++LPF  V +SAKVVGT+ENNIH QT+AS   
Sbjct: 301 LSSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPFVPTSAKVVGTLENNIHLQTVASPLP 360

Query: 361 ----------------------VFCG-----------------------IVKVEFF---- 420
                                 +F G                       IV+  ++    
Sbjct: 361 FSIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPD 420

Query: 421 -----------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVV 445
                                   KTRD EGKK FQF+GP LPENLSDD++VGEFC   +
Sbjct: 421 DLARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSL 480

[Close]

BLAST of HG10006231 vs. ExPASy TrEMBL
Match: A0A6J1DGG8 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020836 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 4.2e-179
Identity = 338/533 (63.41%), Postives = 387/533 (72.61%), Query Frame = 0


Query: 1   MEHF-VAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGG 60
           M+H+ +AI LL+F   +FHFQL VLS ++IPNQDVSYMKFVEDA +LPE EEYDYII+GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDG 120
           GTAGCPLA TLSS FSV LLERGS+PN +PS+L E+GLLN F+ EDD KNPFQRFISEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNA 180
           VEN+RGRVLGG SM+NAGFYSRGH+EFFE AGV+WDMELVEKAY+W EETVVS+PSL +A
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHQEFFEKAGVEWDMELVEKAYQWVEETVVSRPSL-SA 180

Query: 181 WQAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVI 240
            Q  FR+ LLE+G+ PDNGF LR+LVGTRIGGSIFD KGN HGAVELLNKA   N+KV I
Sbjct: 181 LQPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNPHGAVELLNKANPTNIKVAI 240

Query: 241 QAIVQRIIFS-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAH 300
           +  V+RIIFS             GKLH AFIR KGEII+SAGAIGSPQLLLLSG+GPK+ 
Sbjct: 241 RTTVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQ 300

Query: 301 LSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS--- 360
           LSSLKL VVL QP+VGEFM+DNPRFG +++LPF  V +SAKVVGT+ENNIH QT+AS   
Sbjct: 301 LSSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPXVPTSAKVVGTLENNIHLQTVASPLP 360

Query: 361 ----------------------VFCG-----------------------IVKVEFF---- 420
                                 +F G                       IV+  ++    
Sbjct: 361 FSIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPD 420

Query: 421 -----------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVV 445
                                   KTRD EGKK FQF+GP LPENLSDD++VGEFC   +
Sbjct: 421 DLARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSL 480

[Close]

BLAST of HG10006231 vs. ExPASy TrEMBL
Match: A0A5D3CL00 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00890 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 3.5e-178
Identity = 338/524 (64.50%), Postives = 381/524 (72.71%), Query Frame = 0


Query: 9   LLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGGTAGCPLAA 68
           +L+F+ +   FQLGV+S + IPNQD SYMKFV +A+EL ESEEYDYIIIGGGTAGCPLAA
Sbjct: 5   ILLFTLISI-FQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAA 64

Query: 69  TLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVENIRGRVL 128
           TLSS FSV LLERG+DPN YPS+LNEQGLLN F  EDDG+NPFQ FISEDGVEN+RGRVL
Sbjct: 65  TLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVL 124

Query: 129 GGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQAAFRNVL 188
           GGGSM+NAGFYSRGHREFFETAGVDWDMELVEKAYEW EETVVS+PSL + WQAAFR+ L
Sbjct: 125 GGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSL-SPWQAAFRSAL 184

Query: 189 LEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAIVQRIIF 248
           LE G+  DNGFDL++LVGT+ GGSIFDNKGNRHGAVELLNK E +N KV  QA VQRIIF
Sbjct: 185 LEGGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIF 244

Query: 249 S-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAHLSSLKLHVV 308
           +             GKLH AFIR+KGEII+SAGAIGSPQLLLLSGVGPK+ L SLKL VV
Sbjct: 245 TGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVV 304

Query: 309 LDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS------------ 368
           LDQPHVGEFM+DNPRF   ++LPFQ+VASSA+VVGT++NNIH Q  AS            
Sbjct: 305 LDQPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSL 364

Query: 369 -------------VFCG-----------------------IVKVEFF------------- 428
                        +F G                       IV+  ++             
Sbjct: 365 LPPQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGV 424

Query: 429 --------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVATYWHYHGG 445
                          KT+DLEG K FQFLG  LPENL +D++V E+C+  V TYWHYHGG
Sbjct: 425 RKVGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGG 484

[Close]

BLAST of HG10006231 vs. ExPASy TrEMBL
Match: A0A6J1FC90 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444087 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 3.5e-178
Identity = 343/533 (64.35%), Postives = 382/533 (71.67%), Query Frame = 0


Query: 1   MEHFVAIVL-LIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGG 60
           +EH +A +L LI  S+   F L VLS N+IPNQDVSYMK V DA ELPE+E+YDYIIIGG
Sbjct: 4   IEHSMATILPLILISI---FLLRVLSSNTIPNQDVSYMKLVHDATELPENEDYDYIIIGG 63

Query: 61  GTAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDG 120
           GTAGCPLAATLSS FSV LLERGSDPN YPS+LNEQGLLNVF  EDDG+NPFQRF+SEDG
Sbjct: 64  GTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSEDG 123

Query: 121 VENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNA 180
           VE IRGRVLGGGSMVNAGFYSRGH EFFE+AGVDWDMELVEK YEW E+TVVS+P L +A
Sbjct: 124 VEIIRGRVLGGGSMVNAGFYSRGHGEFFESAGVDWDMELVEKGYEWVEKTVVSRPRL-SA 183

Query: 181 WQAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVI 240
           WQ+AFR  LLE G+ PDNGFDLR+LVGT+ GGSIFD  GNRHGAVELLN  E  NLKV +
Sbjct: 184 WQSAFRRALLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAV 243

Query: 241 QAIVQRIIFS-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAH 300
           +A VQ+IIFS             GK H AFIR+KGEII+SAGAIGSPQLLLLSGVGPK+H
Sbjct: 244 RATVQKIIFSDLSAIGVSYSDSKGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSH 303

Query: 301 LSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS--- 360
           LSSLKL VVL QPHVGEFM+DNPRF   ++LPF LVASSA+VVG ++ NIH QT AS   
Sbjct: 304 LSSLKLPVVLHQPHVGEFMSDNPRFSAAIVLPFPLVASSAQVVGILDGNIHLQTFASPAP 363

Query: 361 ----------------------VFCG-----------------------IVKVEFF---- 420
                                 +  G                       IV+  ++    
Sbjct: 364 FFILPTFGLLPPQSTLINPSLALLIGKFTDVLSKGSLHLNSSTDVKNNPIVRFNYYSNPN 423

Query: 421 -----------------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVV 445
                                   KTRDLEG KGF FLGPPLPENLSD +SV E+C+  V
Sbjct: 424 DLAQCVRGLRKVGDLLKTRMMEKIKTRDLEGNKGFLFLGPPLPENLSDISSVEEYCKETV 483

[Close]

BLAST of HG10006231 vs. ExPASy TrEMBL
Match: A0A1S3BSP4 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 3.5e-178
Identity = 338/524 (64.50%), Postives = 381/524 (72.71%), Query Frame = 0


Query: 9   LLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGGTAGCPLAA 68
           +L+F+ +   FQLGV+S + IPNQD SYMKFV +A+EL ESEEYDYIIIGGGTAGCPLAA
Sbjct: 5   ILLFTLISI-FQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAA 64

Query: 69  TLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVENIRGRVL 128
           TLSS FSV LLERG+DPN YPS+LNEQGLLN F  EDDG+NPFQ FISEDGVEN+RGRVL
Sbjct: 65  TLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVL 124

Query: 129 GGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQAAFRNVL 188
           GGGSM+NAGFYSRGHREFFETAGVDWDMELVEKAYEW EETVVS+PSL + WQAAFR+ L
Sbjct: 125 GGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSL-SPWQAAFRSAL 184

Query: 189 LEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAIVQRIIF 248
           LE G+  DNGFDL++LVGT+ GGSIFDNKGNRHGAVELLNK E +N KV  QA VQRIIF
Sbjct: 185 LEGGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIF 244

Query: 249 S-------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKAHLSSLKLHVV 308
           +             GKLH AFIR+KGEII+SAGAIGSPQLLLLSGVGPK+ L SLKL VV
Sbjct: 245 TGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVV 304

Query: 309 LDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIAS------------ 368
           LDQPHVGEFM+DNPRF   ++LPFQ+VASSA+VVGT++NNIH Q  AS            
Sbjct: 305 LDQPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSL 364

Query: 369 -------------VFCG-----------------------IVKVEFF------------- 428
                        +F G                       IV+  ++             
Sbjct: 365 LPPQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGV 424

Query: 429 --------------YKTRDLEGKKGFQFLGPPLPENLSDDNSVGEFCRNVVATYWHYHGG 445
                          KT+DLEG K FQFLG  LPENL +D++V E+C+  V TYWHYHGG
Sbjct: 425 RKVGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGG 484

[Close]

BLAST of HG10006231 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 397.5 bits (1020), Expect = 1.4e-110
Identity = 214/533 (40.15%), Postives = 305/533 (57.22%), Query Frame = 0


Query: 10  LIFSSVLFHFQLGVL-SPNSIP--NQDVSYMKFVEDANELPESEEYDYIIIGGGTAGCPL 69
           L++++++    LGV+   N+ P  N+   +M+F+ +A +    + YDYII+GGGTAGCPL
Sbjct: 9   LLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPL 68

Query: 70  AATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVENIRGR 129
           AATLS +F V LLERG  P   P++++  G L   +  ++  +P Q FISE+GV N RGR
Sbjct: 69  AATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGR 128

Query: 130 VLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQAAFRN 189
           VLGG S +NAGFYSR  ++FFE +G+ WD+  V ++YEW E  +V +P L   WQ A R+
Sbjct: 129 VLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQL-RTWQTAIRD 188

Query: 190 VLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAIVQRI 249
            LLE G+ P NGF L + VGT+IGGS FD  G RH + +LL  A   N++V + A V+R+
Sbjct: 189 ALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERV 248

Query: 250 IFS---------------------GKLHKAFIREKGEIIVSAGAIGSPQLLLLSGVGPKA 309
           + +                     G+ H A IR++GE+I+SAGA+GSPQLL LSG+GP++
Sbjct: 249 LLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRS 308

Query: 310 HLSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTIENNIHFQTIASV- 369
           +LS+  + V LDQPHVG+F+ DNPR G++++ P  +  S  +VVG  E+    +  ++V 
Sbjct: 309 YLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVI 368

Query: 370 ----------------------------------------------FCGIVKVEFFYKTR 429
                                                            +V+  +F   +
Sbjct: 369 PFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQ 428

Query: 430 DLE---------------------------GKKGFQFLGPPLPENLSDDNSVGEFCRNVV 445
           DLE                           G + F+F+G PLP + S+D  + +FCR  V
Sbjct: 429 DLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTV 488

[Close]

BLAST of HG10006231 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 317.0 bits (811), Expect = 2.4e-86
Identity = 197/498 (39.56%), Postives = 261/498 (52.41%), Query Frame = 0


Query: 48  ESEEYDYIIIGGGTAGCPLAATLSSNFSVPLLERGSDP--NPYPSLLNEQGLLNVFSTED 107
           +   YDYI+IGGGTAGCPLAATLS NFSV +LERG  P  N   S L      ++   + 
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADI 118

Query: 108 DGKNPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEW 167
              +  Q F+S DGV N R RVLGGGS +NAGFYSR    F + AG  WD +LV+++Y W
Sbjct: 119 SASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPW 178

Query: 168 GEETVVSQPSLNNAWQAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVE 227
            E  +V QP L   WQ A R+ LLE G++P NGF   ++ GT+IGG+IFD  G RH A E
Sbjct: 179 VEREIVHQPKL-TLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAE 238

Query: 228 LLNKAEFKNLKVVIQAIVQRIIF-----------------SGKLHKAFI--REKGEIIVS 287
           LL  A  + L+V+I A VQ+I+F                  G  H+A +  R+  E+I+S
Sbjct: 239 LLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILS 298

Query: 288 AGAIGSPQLLLLSGVGPKAHLSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSA 347
           +GAIGSPQ+L+LSG+GPK  L  LK+ VVL+  HVG+ MADNP   + +     +  S  
Sbjct: 299 SGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLI 358

Query: 348 KVVGTIENNIHFQTIAS---------VFCGIV--KVEFF---------------YKTRD- 407
           + VG  +  ++ +                GI+  K E F               Y TR+ 
Sbjct: 359 QTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNK 418

Query: 408 ------LEGKKGFQFLGPPL---------------------------------------- 445
                   G    + L  P+                                        
Sbjct: 419 YQLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPCDKQNVHKMLSLSV 478

[Close]

BLAST of HG10006231 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 315.1 bits (806), Expect = 9.1e-86
Identity = 213/568 (37.50%), Postives = 277/568 (48.77%), Query Frame = 0


Query: 1   MEHFVAIVLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGG 60
           M+ F +  L +  S+  H    + S +  PN       F+ DA   P +  YDYIIIGGG
Sbjct: 2   MDRFWSWRLFVALSLFLHSP--ICSSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGG 61

Query: 61  TAGCPLAATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGV 120
           TAGCPLAATLS N SV LLERG  P   P++          S   +  +P QRF+SEDGV
Sbjct: 62  TAGCPLAATLSQNASVLLLERGDSPYNNPNITRLSAFGAALSDLSE-SSPSQRFVSEDGV 121

Query: 121 ENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAW 180
            N R RVLGGGS +NAGFY+R   ++    G  WD  L  ++Y+W E  V  QP +   W
Sbjct: 122 INARARVLGGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGR-W 181

Query: 181 QAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQ 240
           Q A R+ LLEAGI P+NGF   ++ GT+ GG+IFD  GNRH A +LL  A+ K + V++ 
Sbjct: 182 QTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLH 241

Query: 241 AIVQRIIF------------------SGKLHKAFIRE--KGEIIVSAGAIGSPQLLLLSG 300
           A V RI+F                  +G+ H+A+++E    EII+SAG +GSPQLL+LSG
Sbjct: 242 ATVHRILFRTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSG 301

Query: 301 VGPKAHLSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVGTI-------- 360
           VGP A L +  + VV+DQPHVG+ M DNP   V +  P  +  S  +VVG          
Sbjct: 302 VGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEA 361

Query: 361 ----------------------------------ENNIHFQTIASVFCG----------- 420
                                              N++   + A  F G           
Sbjct: 362 AGGENFGGGGGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPL 421

Query: 421 ----------------IVKVEFFYKTRDLE-----------------------GKKGFQF 445
                           IV   +F    DL+                           F++
Sbjct: 422 STGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVSFEY 481

[Close]

BLAST of HG10006231 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 306.6 bits (784), Expect = 3.2e-83
Identity = 197/525 (37.52%), Postives = 260/525 (49.52%), Query Frame = 0


Query: 48  ESEEYDYIIIGGGTAGCPLAATLSSNFSVPLLERGSDP--NPYPSLLNEQGLLNVFSTED 107
           +   YDYI+IGGGTAGCPLAATLS NFSV +LERG  P  N   S L      ++   + 
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADI 118

Query: 108 DGKNPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEW 167
              +  Q F+S DGV N R RVLGGGS +NAGFYSR    F + AG  WD +LV+++Y W
Sbjct: 119 SASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPW 178

Query: 168 GEETVVSQPSLNNAWQAAFRNVLLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVE 227
            E  +V QP L   WQ A R+ LLE G++P NGF   ++ GT+IGG+IFD  G RH A E
Sbjct: 179 VEREIVHQPKL-TLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAE 238

Query: 228 LLNKAEFKNLKVVIQAIVQRIIF-----------------SGKLHKAFI--REKGEIIVS 287
           LL  A  + L+V+I A VQ+I+F                  G  H+A +  R+  E+I+S
Sbjct: 239 LLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILS 298

Query: 288 AGAIGSPQLLLLSGVGPKAHLSSLKLHVVLDQPHVGEFMADNPRFGVNLMLPFQLVASSA 347
           +GAIGSPQ+L+LSG+GPK  L  LK+ VVL+  HVG+ MADNP   + +     +  S  
Sbjct: 299 SGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLI 358

Query: 348 KVVGTIENNIHFQTIAS---------VFCGIV--KVEFF---------------YKTR-- 407
           + VG  +  ++ +                GI+  K E F               Y TR  
Sbjct: 359 QTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNK 418

Query: 408 --------------------------------DLEGKKGFQFLGPPL------------- 445
                                           D      F +   P+             
Sbjct: 419 YQLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVS 478

[Close]

BLAST of HG10006231 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 303.9 bits (777), Expect = 2.1e-82
Identity = 206/558 (36.92%), Postives = 280/558 (50.18%), Query Frame = 0


Query: 8   VLLIFSSVLFHFQLGVLSPNSIPNQDVSYMKFVEDANELPESEEYDYIIIGGGTAGCPLA 67
           ++L++ S+ F F + + +             F++DA   P++  +DYIIIGGGTAGC LA
Sbjct: 41  LILVYISIFFFFLVKLET------------TFMKDATLAPKNASFDYIIIGGGTAGCALA 100

Query: 68  ATLSSNFSVPLLERGSDPNPYPSLLNEQGLLNVFSTEDDGKNPFQRFISEDGVENIRGRV 127
           ATLS N SV +LERG  P   P+  +    +N     +   +  Q FISEDGV N R RV
Sbjct: 101 ATLSQNASVLVLERGGSPYENPTATDMGNSVNTL-LNNTPNSWSQLFISEDGVYNTRPRV 160

Query: 128 LGGGSMVNAGFYSRGHREFFETAGVDWDMELVEKAYEWGEETVVSQPSLNNAWQAAFRNV 187
           LGGGS++N GFYSR   ++ E A  +W+ME VE AYEW E+ +V +P +   WQ AF++ 
Sbjct: 161 LGGGSVINGGFYSRAGNDYVEEA--EWEMEEVEAAYEWVEKKLVFEPQVIE-WQKAFKDG 220

Query: 188 LLEAGIQPDNGFDLRNLVGTRIGGSIFDNKGNRHGAVELLNKAEFKNLKVVIQAIVQRII 247
           LLEAG  PDNGF   ++ GT+IGG+IFD  G+RH A  LL  A    + V + A V +++
Sbjct: 221 LLEAGESPDNGFTYDHIYGTKIGGTIFDRAGHRHTAANLLEYANPNRIVVYLHASVHKVL 280

Query: 248 F------------SGKLHKAFIREK--GEIIVSAGAIGSPQLLLLSGVGPKAHLSSLKLH 307
           F            +G  HKA +  K   E+I+SAGA+GSPQLL+LSGVGP  HL +  ++
Sbjct: 281 FTTEAYEVLFEDANGVFHKANLANKATNEVILSAGALGSPQLLMLSGVGPAVHLEAHGVN 340

Query: 308 -VVLDQPHVGEFMADNPRFGVNLMLPFQLVASSAKVVG---------------------- 367
            +VLDQP VG+ MADNP   V +  P  +  S  + VG                      
Sbjct: 341 PLVLDQPMVGQGMADNPMNFVAIPSPQPVELSLIQAVGITKFDSYIEGLSGLSLSFDITR 400

Query: 368 ---------------TIENNIHFQTIASVFCGI-VKVE---------------------- 427
                          T    I  Q+IA +     VK+E                      
Sbjct: 401 RFFDGVLNLLNETSHTTSRKILTQSIAVLLKSFDVKLEVRMNGGLIFQKVDGPASKGHMK 460

Query: 428 ---------------FFYKTRDLE-------------GKKGFQ---------------FL 445
                          ++ +  DL                K F                 L
Sbjct: 461 LRNTNPRDNPSVTFNYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGVTARELLNLML 520

The following BLAST results are available for this feature:

BLAST of HG10006231 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889314.1	1.7e-182	65.04	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]
XP_038889315.1	6.4e-182	64.47	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]
XP_038889699.1	1.1e-181	65.04	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]
XP_022153314.1	1.6e-180	63.98	(R)-mandelonitrile lyase 1-like [Momordica charantia]	[more]
XP_022153315.1	8.6e-179	63.41	(R)-mandelonitrile lyase 1-like [Momordica charantia]	[more]

BLAST of HG10006231 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52707	3.1e-115	43.13	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
P52706	2.0e-114	42.91	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q945K2	2.2e-113	42.61	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
O50048	2.5e-112	42.45	(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1	[more]
O82784	1.6e-111	40.98	(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1	[more]

BLAST of HG10006231 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIQ0	7.6e-181	63.98	(R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020835 P...	[more]
A0A6J1DGG8	4.2e-179	63.41	(R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020836 P...	[more]
A0A5D3CL00	3.5e-178	64.50	(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A6J1FC90	3.5e-178	64.35	(R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444087 PE...	[more]
A0A1S3BSP4	3.5e-178	64.50	(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=...	[more]

BLAST of HG10006231 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	1.4e-110	40.15	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.2	2.4e-86	39.56	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	9.1e-86	37.50	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	3.2e-83	37.52	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51930.1	2.1e-82	36.92	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 52..307 e-value: 3.2E-25 score: 89.1
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00624	GMC_OXRED_2	coord: 268..282
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00623	GMC_OXRED_1	coord: 125..148
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 373..444 e-value: 9.0E-18 score: 66.3
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 34..354 e-value: 2.7E-76 score: 258.7
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 46..444
IPR012132	Glucose-methanol-choline oxidoreductase	PIRSF	PIRSF000137	Alcohol_oxidase	coord: 35..335 e-value: 3.3E-53 score: 179.3 coord: 359..444 e-value: 4.6E-18 score: 63.2
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 376..443 e-value: 8.2E-17 score: 62.0
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 355..444
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 3..350
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 3..350 coord: 355..444

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10006231.1	HG10006231.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors