HG10005991 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10005991
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGDSL esterase/lipase
LocationChr07: 10719049 .. 10725651 (-)
RNA-Seq ExpressionHG10005991
SyntenyHG10005991
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGGACAAATGATGGTGTATTGTTTAATAATAATTGGAGGATTAATAGAGAGTAAATGGGTTGAAGGAGCATCAACAAAAAAGAAATTAGTTAGATGTTATTTTGTATTTGGAGACTCTCAAGCAGACAATGGAAATAACAACGACATATTGGAAAGGGAATATGGGAATGCGAGAGCTGATTATGAGCCTTATGGCATTGACTTCTCTAAACAAAGGATACCCACTGGCAGGTTCACCAATGGCCGAAATGTTCCTGATTTCATCGGTTTGTCTTCTCATTCCCTTCCTTTTTCTTTTCTTTCTTTTAAAAAAAATAATTAAATTTTACTCTCCAACCTTTTACTTCACTTTCATCATTAGACTAACCCGACTCTAAAAAAATGAAAATCCATATTTGACCTAAGTCCGAACTAACTCTTGGACTTGGCAGCTCATGGATCGAAGTTTAGATCATATTAAAAAATATATATATATTTGATATGATTTGATATTGTCCATATTGAGAATGAACCTTTTCATTTTTCAGCTAAATTTTTAGGGTTTCACGATTACATTCCACCATTTAAGACTACAAAGAGTCGAACTATTCTGAAAGGTGTAAACTATGCATCTGGAGGTGCAGGAATTCTCCATGAAACTGGAAGAACACTGGTAACTTTTTTTTTTAATGTAATGAAGTGGGGATTCAAGCCATAAACTTATTGGTCACTAACACGTAATTTATTATAGGGACAATTACAAATATAGTAATTAGACCAAAATTATTAGCAGATATAACACAATGCAAAAGAATTTGTAAATATAGCAAAATTTAGATCCAACTCTCAGAGTTTATTAGGTTAGGGTCTATCACGGATAGATTTTGCTATATTTGCAATTCTTTAAAAATGTTGCTATGCACTTAATTATTAACTCTAAAAGTGCCACCTACTGCGATTATCTTATATTTATATAAGAGTTAAGTTATGCCCGTTTTGGCGTGGGTAACTAATTTACAACAATATAAAATTTTAGTACTTAAATCACTTTTTTACTTTATATATTTATTTATTTATTTATTTATTACTTTCTATTAGGTCTAGGAGCTTTAAAAAAATCTTATAATCAAAACGTTTATTGTCTTCGTTCCTAACTTAGCATAAAAAAATGTGATTAAATTATTATTATTTTGATATATATACTTTGATTTTTGTTTTATTTTAGTCTCTATATATTTAAATGTTCATATTTACTTTCTGTATCTTCAATAAATCTTAAATTTAGTCTATGTTCTAAGTTTGTCGTTGATTTTTTCAAAAACAACTAAATGTAATATGTATGAGCATTTCCTTTAAAAAAAATTTAATGTCTAAATTTAAGATTTATTTATAGTATAGAGGCCTAAATTTGAACAATTGAAGGTATAAAAACTAAAATAAAATGGAACTCAAATACAGGGATCAAAATGGTATTTTTACTAAAAATAATTTATACTCCTATCATTCATTATTTTTCCTCTCATCATTTATTTGAAACTTGAGACAATGTGACATGACCTTTACTTTTATTCGAGTAGGTATGGATTTCTTTTAGTTCAACATGTATGTGGGTGGAAGGCGTTTCAAATCTCTTAACACCTTGGTGATTAATTGTACATGTGTATGTGTGTGACGAAGTTGTTGCACTGGGCTGAAGTTGATGATAATTAGAGATGTTTACCATTTGATAAATATTTAATTTTCAATTTTTGAAAATTAAGCAACTTATAAATAATTACTTTCACTCATAATTCTTTTGTTATCAACTTTCTACATATGTTTTAAAAAACTATACCCAATTTCGAAAACTTAAAAAAAAAAAATGTTTTCAAAAGTTTGTTTATGTTGATTCAAATAAAGTTGAACCATGATGGTAACTTACAATTTAGATGAAACAAATATAGTAGAAAGCTAAAAACAAAATCGTTATCGAACTGGAGATTGAGGGACCGCTTGGATTGACTTTCCAAATGTTACCTTTTTTACATTTATTTAAACACTCAGAAAGTCAATCCAAACGAACCATAAATGTATGCTAACTCTATATATATGTTTATGTTTGTAGGGGCGAGTATCAAGCATCAAGAAACAATTGGAAAATCACAATTACACAATTTCACAAATGCATGGGTTATTGGGAAGTGAAATTAAAATTAGAAGATATCTTAAAAGATGTTTATATACAGTTCAAATTGGAAGCAATGATTACCTTAATAATTATTTTATGCCAAAGCTCTACATGACAAGTACTCGATATACTCCACAAGCATATGCCATTGCCCTAAACAAATACCTCTCACGCCAATTGAAGGTTAGTTTTCTCAAAAATAATGGTAACATTCTCTTTTATTCTTTCACTAAATCCTAAAATAAGATTATATACATCATTATCTTTACATATCTATGAATTTCTTTATTTTGATATCTATTTTTTTTTCGGGATGTTTCAAAAATAATAAGTCAGAATTTGAAAACTAAAGGGTCTGTTTGATTGGAAGTTAGAGATTATGTTTTATGTTTTTAGGTTCCTGAGTTCGGTAAATATGTGTTTGTCAAGCAACCTGAATTCTGTTTCTGAAAATTGCTTTTGGATTCTGTGATCCAAATAGTGTAAAATTTGAAAACAATATTTTTAAGTTTTGATTGTGTCTAGATTTTGGATCTTAATTAAATTTTAAAATGCAGAATTATATTAAATAAAGATATGAACACCTAGAAATACAATATGTCATGTTATAAACTCAAATTATTTTAAAAAAATTAAAATATGTATTATAAATGGTATAATATTTAAAAATAATAATTATATAAAAAAAATTAGGGAAATTACTATAAACAGAAAAAATATCAAACTATTTACAAATATAGAAAAATTTCACTATCTATCAAAGATAGACTGCGATAGACTTCTATCGCAAAAATTTTATCACGGTCTATCGCTGATAAACAATGAAATTTTTCTATATTTGTAAATAGTTTGACTCATTTTTCTATATTTGAAAATAACCCAAAAATTTAACATAAAACATGTTCATAGATAAATTAATAATAGTTTATTGTCAAATAATATTTAATGGGTAACTATAACTAGTTTTATAATTTATAAATATATGATAAAATATATTTTAAACAATTACTTAAATTATAATCCAATTTTTGAACTGCTACCAAACACATATATGAAAACATGAAATACAACTCTATTTTCATAAATTTCTATTTTCTTATTTTCTACCAAACAGACCCTAAACAAATATAGTTTTATTTTTAAAATATTCTTTTTGATATTTTAGTTTGGCTCTGAATTCAAATGTTTCTTAAAGAAAATTAAAATCGTTTAGAGATTTATGAGGAAAAAAACACAATTTTCAAAAATAAGAAACAAAAGATTAAGACCATAACCGTTTGATTTCTCCGTTTTTAAATTTAAGCTTATAAACACCGACTTTCACTGGTTAGTTTCTTTATTTGTTGTTAACTTTTGACTAATACTTGCAAAAAACCAAGTAAAACTTTGAAAACTCAATAAAGTAGTCATTAAAAACTTGTTTTTCCCTTTGGGATGTGACTAAGAATTCAAATGTTTTTTTTTTAAAAAAAAAAAAGGCGAAAACTTAGGCCCCTTTAATAACCATATAACTTTTGGTTGCTAGAGTTTTGAAAATTAAGCCTATAAATATTCCACTTATTGGTTTCTTTGTTTTAATTGTTTACATTTTAGGAGTGTTTTCAAAATCCAAACCAAATTTTACCAAAAAGAGTGATTTTTAAAACTTTGTTTATTTAATTTTTTTTTAAAAATATTTTGGCTAAAAATTCATATGTTTATTTAAGAAAGATGTAAAATATGACTAAGAAATTGTTAGAAAACAAGTACAATTTCCAATAACAAAAAAGAATCAAATAGTTACCAAATATGACTTAATTAATAAGTAAATTGGGATAACACAAGCATAAATTTTAAAAACAGAAAACTAAACACGAAAACATTATCAAATGAAGTCTAAAAAGTTATGAAACAAACTCTAAGCTAATTAATTTACACTCAATTTAACTATATCAATACAACACTCTTTTCACTTGTAAACTTTTAAAGAGAAAATGACATTATACGAATTATAATTCAAATATATGAGACCTTGTATTTTGTTTTTGTCATAGTTAAATTATTGAATAATATTGTTGGGAAATACATTAATTAATTGAAAAATTTTATGGATAGAAAAAATGTTAAACTATTTATAGAAAATAGCAAAAAAAAATACTGATAGACATTGGTAGACGTCTATCCGCATCTATCAGCGATAGACTTGTATCATTTCTATCACTGATATACTCTGGTAGACTTCTATCACTGTCTATTACAACTATATAAAAAAATTTCTATTTTGTGTAAATAATTTTTCTTATTTTTTTATTTTTAAAAACTCCCCTTAATTAATTATATCGTAGATTCTATATGAGCAAGGAGCAAGAAAGGTGGCCATTTTTGGAGTGGGATCTATAGGTTGTACTCCATATGCAAGGGCAAACTTTGAGCACAAAGGATTTCCATGTGTTGACAAAATCAACAGTGCTGTTCAACTTTTCAATACTGGCCTTAAGTCTCTTGTTCAACAGCTTAATATCAACTTCCCTGCTGCTAAGTTTACCTTCATTGATGTCTTCAAAATTTCCACTGTTAACCCTTCAAATTATGGTAAAACTTTTCTTTCAAATTCTTATCTTCTTAATTATAGACAGAATTTAATTAGTATTCATTCAACTAAATAGGCCACAGATAAATTTTTAGTTTGATTTTTTCTATATATGGGATAAATTATACAAATTAAATATCCTTGGGGGTGGGAACATTATTATTATTTTAGACATACATTTGAACTTTCAAAATTTTTATTTATACCCTTAAACTTTTAAAATAAGTTTAAAAAATACTCTTAACATTAGTTTTTTATAGAAACTATTAGTACTATGTTCCAAAAGTATGCCGAACGTTTAAAAGTTTAATTAATACTCTTAGACTTTCGTTAGTACCTTTCTCTTCCATTTTCTCTCTATCTTTTATTTCTTCCCTTTTTTAAAGTTGTTTTTTTTCTCACTATAGATCATTTATTTTAATGAAATTTCTAATTTTGGAAAAATTACTATAAACAGAAAAAATATCAAACCATTTACAAATATAGAAAAATTTTACTGTCTATCAGTGATAGACCGCGATAGAATTCTATCGCTTTCTATTGCTCAAGCAAATAAATTTTATCGTTTGAGCGATAGAAAGCGATAGAATTCTATCGCAATCTATCAGTCTGACTGTAAAATTTTTCTATATTTGTAAATAGTTTAACTCATTTTTTCTATATTTGAAAAGAGTCATTTTTAATAACTAAAGTTGTTATTTTCTTTTTCTAAAATTGTAGTCTTATGAATTATAATTTTTGTAGTGTACCTCTTTGTGTTCCTACTATTCTTTTTTTTCAAGAAAGCTTTTTTTAGAACAATTGCCAAACGACCTTTTGATCGTTTCTTTCCCTTTCTGATTTAACTTAGAAACTATTTCATGATATCTGTAGTTTTTTTCTTTTCGAATTTACTAATTATTCTATATATTAAATTTAGATAATAGAATGCATTATATCAAGTTATGCTTTCGAACTAAAAAAATGCATTTAAAACAAAGAAACATTGTTTGTCTCTTTTTTTTTTTTTTAAATAACAAAAACATTTTAAAAAATCATCATTACCAAATTAGTTTGGAAAATTTTCACACATAGAAAAAATATCAAACTATTTATAAATATCGAAAAATTTCACTATTTATCAGTGATAGACTGCGTAGAATTCTATCGCTTTCTATCGCTCATGCAAATAAATTATATCGCTTGAGCGATAGAAAGCGATAGAATTCTATCACGGTCTATTACTGATAGACTGTAAAATTTTTCTATATTTATAAATAGTTTGGCTCATTTTTTTATATTTGAAAACAACCATATAAAAAATGTCAAACTATTTACAAAAAATAGCAAAAAAAATGTTGATAGACATTGATAGACTTCTATAAGCATCTATCAGTGATCGACTTCTATCAGCATCTATCACTGATAGATCATAATAGACTTCTATCAGCGTCTATCACAACTATATATCTAAAAAATTTGCTATTTTGTATAAATAGTTTTCCTGATTTTTCTATTTTATAAAATTCTCCAATTAGTTTTCTATATAATAAATAAATAAATAAAGAGCTACTTGGAAACATGATATATTTTTGTACCATTCAGACCGTTGTTGAATAAAAAATTGTTATATGATATATATATTTTGTGTTTTCATTTTAAAATTTTGCATATATGCAGGTAAAATGGTTCTAGATGCTCCATGCTGTGAAGTTGAAGTAGGGGGAATGCAATGTGCTCCTTTTGGAAAAGTGTGTGAAAATAGAAGGAATTACATGTTTTGGGATGGAGTTCATCCAACTGAAATTGGATTTAGATTAGTAGCAAGTAGAGCCTTTAATGCTAAACAACCTGGTGATACTTATCCCTGTGATATTAACCATCTTCGTCACCTATCTACCTAA

mRNA sequence

ATGAAGGGACAAATGATGGTGTATTGTTTAATAATAATTGGAGGATTAATAGAGAGTAAATGGGTTGAAGGAGCATCAACAAAAAAGAAATTAGTTAGATGTTATTTTGTATTTGGAGACTCTCAAGCAGACAATGGAAATAACAACGACATATTGGAAAGGGAATATGGGAATGCGAGAGCTGATTATGAGCCTTATGGCATTGACTTCTCTAAACAAAGGATACCCACTGGCAGGTTCACCAATGGCCGAAATGTTCCTGATTTCATCGCTAAATTTTTAGGGTTTCACGATTACATTCCACCATTTAAGACTACAAAGAGTCGAACTATTCTGAAAGGTGTAAACTATGCATCTGGAGGTGCAGGAATTCTCCATGAAACTGGAAGAACACTGGGGCGAGTATCAAGCATCAAGAAACAATTGGAAAATCACAATTACACAATTTCACAAATGCATGGGTTATTGGGAAGTGAAATTAAAATTAGAAGATATCTTAAAAGATGTTTATATACAGTTCAAATTGGAAGCAATGATTACCTTAATAATTATTTTATGCCAAAGCTCTACATGACAAGTACTCGATATACTCCACAAGCATATGCCATTGCCCTAAACAAATACCTCTCACGCCAATTGAAGGTTCCTGAGTTCGGAGCAAGAAAGGTGGCCATTTTTGGAGTGGGATCTATAGGTTGTACTCCATATGCAAGGGCAAACTTTGAGCACAAAGGATTTCCATGTGTTGACAAAATCAACAGTGCTGTTCAACTTTTCAATACTGGCCTTAAGTCTCTTGTTCAACAGCTTAATATCAACTTCCCTGCTGCTAAGTTTACCTTCATTGATGTCTTCAAAATTTCCACTGTTAACCCTTCAAATTATGGTAAAATGGTTCTAGATGCTCCATGCTGTGAAGTTGAAGTAGGGGGAATGCAATGTGCTCCTTTTGGAAAAGTGTGTGAAAATAGAAGGAATTACATGTTTTGGGATGGAGTTCATCCAACTGAAATTGGATTTAGATTAGTAGCAAGTAGAGCCTTTAATGCTAAACAACCTGGTGATACTTATCCCTGTGATATTAACCATCTTCGTCACCTATCTACCTAA

Coding sequence (CDS)

ATGAAGGGACAAATGATGGTGTATTGTTTAATAATAATTGGAGGATTAATAGAGAGTAAATGGGTTGAAGGAGCATCAACAAAAAAGAAATTAGTTAGATGTTATTTTGTATTTGGAGACTCTCAAGCAGACAATGGAAATAACAACGACATATTGGAAAGGGAATATGGGAATGCGAGAGCTGATTATGAGCCTTATGGCATTGACTTCTCTAAACAAAGGATACCCACTGGCAGGTTCACCAATGGCCGAAATGTTCCTGATTTCATCGCTAAATTTTTAGGGTTTCACGATTACATTCCACCATTTAAGACTACAAAGAGTCGAACTATTCTGAAAGGTGTAAACTATGCATCTGGAGGTGCAGGAATTCTCCATGAAACTGGAAGAACACTGGGGCGAGTATCAAGCATCAAGAAACAATTGGAAAATCACAATTACACAATTTCACAAATGCATGGGTTATTGGGAAGTGAAATTAAAATTAGAAGATATCTTAAAAGATGTTTATATACAGTTCAAATTGGAAGCAATGATTACCTTAATAATTATTTTATGCCAAAGCTCTACATGACAAGTACTCGATATACTCCACAAGCATATGCCATTGCCCTAAACAAATACCTCTCACGCCAATTGAAGGTTCCTGAGTTCGGAGCAAGAAAGGTGGCCATTTTTGGAGTGGGATCTATAGGTTGTACTCCATATGCAAGGGCAAACTTTGAGCACAAAGGATTTCCATGTGTTGACAAAATCAACAGTGCTGTTCAACTTTTCAATACTGGCCTTAAGTCTCTTGTTCAACAGCTTAATATCAACTTCCCTGCTGCTAAGTTTACCTTCATTGATGTCTTCAAAATTTCCACTGTTAACCCTTCAAATTATGGTAAAATGGTTCTAGATGCTCCATGCTGTGAAGTTGAAGTAGGGGGAATGCAATGTGCTCCTTTTGGAAAAGTGTGTGAAAATAGAAGGAATTACATGTTTTGGGATGGAGTTCATCCAACTGAAATTGGATTTAGATTAGTAGCAAGTAGAGCCTTTAATGCTAAACAACCTGGTGATACTTATCCCTGTGATATTAACCATCTTCGTCACCTATCTACCTAA

Protein sequence

MKGQMMVYCLIIIGGLIESKWVEGASTKKKLVRCYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKFLGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLKVPEFGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFIDVFKISTVNPSNYGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHLST
Homology
BLAST of HG10005991 vs. NCBI nr
Match: XP_038891276.1 (GDSL esterase/lipase At1g29670-like [Benincasa hispida])

HSP 1 Score: 640.6 bits (1651), Expect = 8.5e-180
Identity = 311/370 (84.05%), Postives = 337/370 (91.08%), Query Frame = 0

Query: 1   MKGQ--MMVYCLIII--GGLIESKWVEGA-STKKKLVRCYFVFGDSQADNGNNNDILERE 60
           MKG+  M+ YCLIII  G L+E+K+V+GA S+ KK V CYFVFGDSQADNGNNND+LERE
Sbjct: 1   MKGELLMVYYCLIIIIGGLLLENKYVDGASSSSKKKVSCYFVFGDSQADNGNNNDMLERE 60

Query: 61  YGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKFLGFHDYIPPFKTTKSRTILKGV 120
           YGNARADY+PYGIDFSKQ IPTGRFTNGRNVPDFIAKFLGF DYIPPFK TKSRTILKGV
Sbjct: 61  YGNARADYKPYGIDFSKQSIPTGRFTNGRNVPDFIAKFLGFRDYIPPFKITKSRTILKGV 120

Query: 121 NYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIRRYLKRCLYTVQI 180
           NYASGGAGIL ETGRTLG+VSSIKKQLENHNYTISQMHGLLGS+ KI  YLKRCLYT+QI
Sbjct: 121 NYASGGAGILRETGRTLGQVSSIKKQLENHNYTISQMHGLLGSKTKITSYLKRCLYTIQI 180

Query: 181 GSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLKV-PEFGARKVAIFGVGSIGCT 240
           GSNDYLNN+FMPKLY TS++Y+P+AYAIALNK LS QLK+  + GARKVAIFGVGSIGCT
Sbjct: 181 GSNDYLNNFFMPKLYQTSSQYSPKAYAIALNKELSTQLKILYDQGARKVAIFGVGSIGCT 240

Query: 241 PYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFIDVFKISTVNPSN 300
           PYARANFEH+G PCVDK+NSA+QLFN GLKSLVQQLN N PAAKFTFIDVFKIST++PS+
Sbjct: 241 PYARANFEHRGLPCVDKMNSAIQLFNDGLKSLVQQLNTNLPAAKFTFIDVFKISTIDPSD 300

Query: 301 YGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTEIGFRLVASRAFNAKQPG 360
           YGK VLDAPCCEVEVGGMQC PFGKVC NR +YMFWDGVHPTEIGFRLVASRAFNAKQ G
Sbjct: 301 YGKKVLDAPCCEVEVGGMQCDPFGKVCGNRMDYMFWDGVHPTEIGFRLVASRAFNAKQAG 360

Query: 361 DTYPCDINHL 365
           DTYP DINHL
Sbjct: 361 DTYPFDINHL 370

BLAST of HG10005991 vs. NCBI nr
Match: XP_011649033.1 (GDSL esterase/lipase At1g29670 [Cucumis sativus] >XP_031737227.1 GDSL esterase/lipase At1g29670 [Cucumis sativus] >KGN61342.1 hypothetical protein Csa_005903 [Cucumis sativus])

HSP 1 Score: 596.7 bits (1537), Expect = 1.4e-166
Identity = 290/385 (75.32%), Postives = 320/385 (83.12%), Query Frame = 0

Query: 1   MKGQMM---VYCLIIIGGLIESKWVEGASTKKK----------LVRCYFVFGDSQADNGN 60
           MKG+++    YC +++  +I       AST  K          +  CYFVFGDSQADNGN
Sbjct: 1   MKGELLKVYYYCAVVVVVIINGVVDGAASTSSKHKGKGGSGRGICSCYFVFGDSQADNGN 60

Query: 61  NNDILEREYGNARADYEPYGIDFSKQR---IPTGRFTNGRNVPDFIAKFLGFHDYIPPFK 120
           NND+LEREYG ARADY+PYGIDFS      IPTGRFTN RNVPDFIAKFLGF DYIPPF+
Sbjct: 61  NNDMLEREYGRARADYKPYGIDFSSSSSSYIPTGRFTNARNVPDFIAKFLGFDDYIPPFR 120

Query: 121 TTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIRR 180
           TTKSRTILKG NYASGGAGIL ETGRTLG+VSSIKKQLENHNYTISQMH LLGS  KI R
Sbjct: 121 TTKSRTILKGANYASGGAGILRETGRTLGQVSSIKKQLENHNYTISQMHTLLGSSSKINR 180

Query: 181 YLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLK-VPEFGARKV 240
           YLK CLYTVQIGSNDYLNNYFMPK+YMTST+Y+PQA+A ALNK LS+ LK +  +GARKV
Sbjct: 181 YLKECLYTVQIGSNDYLNNYFMPKIYMTSTQYSPQAFATALNKNLSQHLKNLYNYGARKV 240

Query: 241 AIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFID 300
           AIFGVGSIGCTPYAR NFEH G PCVD+INSA+QLFN+GLKSLVQ LN N P+AKFTFID
Sbjct: 241 AIFGVGSIGCTPYARENFEHTGLPCVDEINSAIQLFNSGLKSLVQHLNANLPSAKFTFID 300

Query: 301 VFKISTVNPSNYGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTEIGFRLV 360
           VF+ISTV+P NYGKMVLDAPCCEV  G MQC+PFGKVC+NR +YMFWDGVHPTE GF+LV
Sbjct: 301 VFQISTVDPLNYGKMVLDAPCCEVGAGAMQCSPFGKVCKNRGDYMFWDGVHPTESGFKLV 360

Query: 361 ASRAFNAKQPGDTYPCDINHLRHLS 369
           ASRAFNAKQPG+ YP DINHL HLS
Sbjct: 361 ASRAFNAKQPGEAYPFDINHLVHLS 385

BLAST of HG10005991 vs. NCBI nr
Match: XP_008441762.1 (PREDICTED: GDSL esterase/lipase At1g29670-like [Cucumis melo] >KAA0051383.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK23839.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 576.2 bits (1484), Expect = 2.0e-160
Identity = 286/386 (74.09%), Postives = 318/386 (82.38%), Query Frame = 0

Query: 1   MKGQMM---VYCLIIIGGLIESKWVEGA---STKKK------LVRCYFVFGDSQADNGNN 60
           MKG+++    YC  ++  +I +  V+GA   ++KKK         CYFVFGDSQADNGNN
Sbjct: 1   MKGELLKGYYYCTAVVVVVIINGVVDGAASLASKKKGGGGSDACSCYFVFGDSQADNGNN 60

Query: 61  NDILEREYGNARADYEPYGIDF-----SKQRIPTGRFTNGRNVPDFIAKFLGFHDYIPPF 120
           ND+LEREYG ARADY+PYGIDF     S   IPTGRFTNGRNVPDFIA+FLGF DYIPPF
Sbjct: 61  NDMLEREYGRARADYKPYGIDFPPSSSSSSFIPTGRFTNGRNVPDFIAQFLGFRDYIPPF 120

Query: 121 KTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIR 180
           +TTKS+TILKG NYASGGAGIL ETGRTLG VSSIKKQLENHNYTISQM  L GS  K++
Sbjct: 121 RTTKSQTILKGANYASGGAGILLETGRTLGEVSSIKKQLENHNYTISQMQRLFGSSSKLK 180

Query: 181 RYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLKV-PEFGARK 240
            YLK CLYTVQIGSNDYLNNYFMP +YMTST+Y+PQA+A ALN+ LSRQLK+  E GARK
Sbjct: 181 SYLKECLYTVQIGSNDYLNNYFMPTIYMTSTQYSPQAFATALNEDLSRQLKILYEQGARK 240

Query: 241 VAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFI 300
           VAIFGVGSIGCTPYARANFEH G PCVDKIN+A+QLFN GLKSLV+ LN N P+AKFTFI
Sbjct: 241 VAIFGVGSIGCTPYARANFEHIGLPCVDKINTAIQLFNAGLKSLVEHLNANLPSAKFTFI 300

Query: 301 DVFKISTVNPSNYGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTEIGFRL 360
           DVFKISTV+P NYGK+ LDAPCCEV  G MQC PFGKVCENRR+YMF+DGVHPTE GF L
Sbjct: 301 DVFKISTVDPLNYGKVNLDAPCCEVGAGEMQCTPFGKVCENRRDYMFFDGVHPTENGFEL 360

Query: 361 VASRAFNAKQPGDTYPCDINHLRHLS 369
           VAS AFNAKQP + YP DINHL HLS
Sbjct: 361 VASSAFNAKQPDEAYPFDINHLVHLS 386

BLAST of HG10005991 vs. NCBI nr
Match: KAG6598965.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 489.6 bits (1259), Expect = 2.4e-134
Identity = 240/390 (61.54%), Postives = 279/390 (71.54%), Query Frame = 0

Query: 1   MKGQMMVYCLIIIGGLIESKWVEGASTK----------------------KKLVRCYFVF 60
           MKGQ +V   ++      S WV+GAS+                       K  V CYFV 
Sbjct: 1   MKGQSLVVLCLLTIACFASNWVDGASSSRIKSRTKSRTKSKPKSISTTQTKTKVGCYFVL 60

Query: 61  GDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKFLGFHD 120
           GDSQADNGNNN ++E  YGNARADY+PYG DF+++  PTGRFTNGRN PDFIA +LGF  
Sbjct: 61  GDSQADNGNNN-VMEMAYGNARADYKPYGCDFNREGTPTGRFTNGRNTPDFIATYLGFRH 120

Query: 121 YIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGS 180
           YI PF T K R IL GVNYASGGAGIL ETGRTLG+V SI KQL NHN TIS++ GLLG+
Sbjct: 121 YISPFTTIKGREILDGVNYASGGAGILPETGRTLGKVLSIHKQLVNHNITISRIRGLLGT 180

Query: 181 EIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLKV-PE 240
                 +L  CLYTVQIGSNDYLNNYFMPK Y TS +YTPQ +A  LN  L  +LKV  E
Sbjct: 181 RSATSTHLSSCLYTVQIGSNDYLNNYFMPKFYKTSAQYTPQQFATVLNNQLYTELKVLYE 240

Query: 241 FGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAA 300
            GARKVA+FGVGSIGCTPYARANFE+KG  CVDKIN+A+QLFN GLK+LV+Q N     A
Sbjct: 241 QGARKVAVFGVGSIGCTPYARANFENKGGGCVDKINNAIQLFNDGLKALVRQFNTKMAGA 300

Query: 301 KFTFIDVFKISTVNPSNYGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTE 360
           KFT+IDVFKIS+ +P+  G+MVL+APCCEV+ G +QC   G++C NR  YMFWD VHPTE
Sbjct: 301 KFTYIDVFKISSTSPATSGRMVLNAPCCEVDAGQVQCTRLGRICANRMEYMFWDAVHPTE 360

Query: 361 IGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
           +G   +ASRAF AK+ GD YP DIN L HL
Sbjct: 361 VGISALASRAFKAKEAGDAYPYDINQLVHL 389

BLAST of HG10005991 vs. NCBI nr
Match: XP_022973923.1 (GDSL esterase/lipase At1g29670-like [Cucurbita maxima])

HSP 1 Score: 489.2 bits (1258), Expect = 3.1e-134
Identity = 240/380 (63.16%), Postives = 277/380 (72.89%), Query Frame = 0

Query: 1   MKGQMMVYCLIIIGGLIESKWVEGASTK---------------KKLVRCYFVFGDSQADN 60
           MKGQ +V   ++      S WV+GAS +               K  V CYFV GDSQADN
Sbjct: 1   MKGQSLVVLCLLTTACFASSWVDGASPRIKSRTRPKSNATTQTKTKVGCYFVLGDSQADN 60

Query: 61  GNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKFLGFHDYIPPFKT 120
           GNNN ++E  YGNARADY+PYG DF+++  PTGRFTNGRN PDFIA +LGF  YI PF T
Sbjct: 61  GNNN-VMEMAYGNARADYKPYGCDFNREGTPTGRFTNGRNAPDFIATYLGFRHYISPFTT 120

Query: 121 TKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIRRY 180
            K R IL GVNYASGGAGIL ETGRTLG+V SI KQLENHN TISQ+ GLLG+      +
Sbjct: 121 IKGREILDGVNYASGGAGILPETGRTLGQVLSIHKQLENHNITISQIRGLLGTRSATTTH 180

Query: 181 LKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLKV-PEFGARKVA 240
           L  CLYTVQIGSNDYLNNYFMP+ Y TS ++TPQ +A  LN  L  +LKV  E GARKVA
Sbjct: 181 LNSCLYTVQIGSNDYLNNYFMPQFYKTSAQFTPQQFATVLNNRLYTELKVLYEQGARKVA 240

Query: 241 IFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFIDV 300
           +FGVGSIGCTPYARANFE+KG  CVDKIN+A+QLFN GLK+LV QLN     AKFT+IDV
Sbjct: 241 LFGVGSIGCTPYARANFENKGGGCVDKINNAIQLFNDGLKALVLQLNTKMAGAKFTYIDV 300

Query: 301 FKISTVNPSNYGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTEIGFRLVA 360
           FKIS+  PS  G+MVL+APCCEV+ G +QC   G++C NR  YMFWD VHPTE+G   +A
Sbjct: 301 FKISSTIPSTSGRMVLNAPCCEVDAGQVQCTRLGRICGNRMEYMFWDAVHPTEVGISALA 360

Query: 361 SRAFNAKQPGDTYPCDINHL 365
           SRAF AK+ GD YP DIN L
Sbjct: 361 SRAFKAKEAGDAYPYDINQL 379

BLAST of HG10005991 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.5e-86
Identity = 165/342 (48.25%), Postives = 216/342 (63.16%), Query Frame = 0

Query: 32  VRCYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIA 91
           V C+FVFGDS  DNGNNN ++      AR++Y PYGIDF     PTGRF+NG+   D IA
Sbjct: 30  VPCFFVFGDSLVDNGNNNGLI----SIARSNYFPYGIDFGG---PTGRFSNGKTTVDVIA 89

Query: 92  KFLGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQ 151
           + LGF+ YIP + T   R IL GVNYAS  AGI  ETGR LG+  S   Q+ N+  T+SQ
Sbjct: 90  ELLGFNGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQRISFSGQVRNYQTTVSQ 149

Query: 152 MHGLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSR 211
           +  LLG E +   YLKRC+Y+V +GSNDYLNNYFMP  Y +S ++TP+ YA  L    S 
Sbjct: 150 VVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQFTPEQYANDLISRYST 209

Query: 212 QLK-VPEFGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQL 271
           QL  +  +GARK A+ G+G++GC+P A A     G  CVD+INSA Q+FN  L+SLV QL
Sbjct: 210 QLNALYNYGARKFALSGIGAVGCSPNALAG-SPDGRTCVDRINSANQIFNNKLRSLVDQL 269

Query: 272 NINFPAAKFTFID---VFKISTVNPSNYGKMVLDAPCCEV--EVGGMQCAPFGKVCENRR 331
           N N P AKF +I+   +F+    NP+ +G  V +A CC +    G + C P  + C +R 
Sbjct: 270 NNNHPDAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIGRNAGQITCLPGQRPCRDRN 329

Query: 332 NYMFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
            Y+FWD  HPTE    ++A R++NA+   D YP DI+ L  L
Sbjct: 330 AYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDISRLAQL 363

BLAST of HG10005991 vs. ExPASy Swiss-Prot
Match: Q9C7N5 (GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 7.6e-83
Identity = 169/378 (44.71%), Postives = 222/378 (58.73%), Query Frame = 0

Query: 1   MKGQMMVYCLIIIGGLIESKWV--EGASTKKKL---VRCYFVFGDSQADNGNNNDILERE 60
           M+  +  +CL+       S WV   G   K K    V CYF+FGDS  DNGNNN    R 
Sbjct: 1   MESYLRKWCLV-------SVWVLLLGLGFKVKAEPQVPCYFIFGDSLVDNGNNN----RL 60

Query: 61  YGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKFLGFHDYIPPFKTTKSRTILKGV 120
              ARADY PYGIDF     PTGRF+NGR   D + + LGF +YIP + T   + IL+GV
Sbjct: 61  RSIARADYFPYGIDFGG---PTGRFSNGRTTVDVLTELLGFDNYIPAYSTVSGQEILQGV 120

Query: 121 NYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIRRYLKRCLYTVQI 180
           NYAS  AGI  ETG  LG+  +   Q+EN+  T++Q+  +LG E     YLKRC+Y+V +
Sbjct: 121 NYASAAAGIREETGAQLGQRITFSGQVENYKNTVAQVVEILGDEYTAADYLKRCIYSVGM 180

Query: 181 GSNDYLNNYFMPKLYMTSTRYTPQAYAIAL-NKYLSRQLKVPEFGARKVAIFGVGSIGCT 240
           GSNDYLNNYFMP+ Y TS +YTP+ YA  L ++Y  +   +  +GARK A+ G+G+IGC+
Sbjct: 181 GSNDYLNNYFMPQFYSTSRQYTPEQYADDLISRYRDQLNALYNYGARKFALVGIGAIGCS 240

Query: 241 PYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFID---VFKISTVN 300
           P A A     G  CV++INSA ++FN  L S+VQQLN     A FT+I+    F+    N
Sbjct: 241 PNALAQGSQDGTTCVERINSANRIFNNRLISMVQQLNNAHSDASFTYINAYGAFQDIIAN 300

Query: 301 PSNYGKMVLDAPCCEVEVGGMQ--CAPFGKVCENRRNYMFWDGVHPTEIGFRLVASRAFN 360
           PS YG    +  CC +   G Q  C P    C NR  Y+FWD  HP+      +A R++N
Sbjct: 301 PSAYGFTNTNTACCGIGRNGGQLTCLPGEPPCLNRDEYVFWDAFHPSAAANTAIAKRSYN 360

Query: 361 AKQPGDTYPCDINHLRHL 368
           A++  D YP DI+ L  L
Sbjct: 361 AQRSSDVYPIDISQLAQL 364

BLAST of HG10005991 vs. ExPASy Swiss-Prot
Match: Q93YW8 (GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 9.3e-81
Identity = 157/340 (46.18%), Postives = 204/340 (60.00%), Query Frame = 0

Query: 34  CYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKF 93
           CYF+FGDS  D+GNNN    R    ARA+Y PYGIDF  Q  PTGRF+NG+   D I + 
Sbjct: 28  CYFIFGDSLVDSGNNN----RLTSLARANYFPYGIDF--QYGPTGRFSNGKTTVDVITEL 87

Query: 94  LGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMH 153
           LGF DYI P+   +   IL+GVNYAS  AGI  ETGR LG   +   Q+ NH  T+SQ+ 
Sbjct: 88  LGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVSQVV 147

Query: 154 GLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQL 213
            +LG E +   YL +C+Y++ +GSNDYLNNYFMP  Y T ++Y+P AYA  L    + QL
Sbjct: 148 NILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYTEQL 207

Query: 214 KVP-EFGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNI 273
           ++    GARK A+ G+G+IGC+P   A     G  C ++INSA ++FN+ L SLV   N 
Sbjct: 208 RIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDHFNQ 267

Query: 274 NFPAAKFTFID---VFKISTVNPSNYGKMVLDAPCCEV--EVGGMQCAPFGKVCENRRNY 333
           N P AKFT+I+   +F+    NPS YG  V +A CC V    G + C P    C NR  Y
Sbjct: 268 NTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRDEY 327

Query: 334 MFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
           +FWD  HP E    ++ SR+F  +   D +P DI  L  L
Sbjct: 328 VFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQLARL 361

BLAST of HG10005991 vs. ExPASy Swiss-Prot
Match: Q9FK75 (GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.5e-78
Identity = 153/340 (45.00%), Postives = 205/340 (60.29%), Query Frame = 0

Query: 34  CYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKF 93
           CYF+FGDS  DNGNNN +       ARA+Y PYGIDF+    PTGRF+NG    D IA+ 
Sbjct: 29  CYFIFGDSLVDNGNNNQL----QSLARANYFPYGIDFAAG--PTGRFSNGLTTVDVIAQL 88

Query: 94  LGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMH 153
           LGF DYI P+ + + + IL+GVNYAS  AGI  ETGR LG   +   Q+ NH  T+SQ+ 
Sbjct: 89  LGFEDYITPYASARGQDILRGVNYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVV 148

Query: 154 GLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQL 213
            +LG + +   YL +C+Y++ +GSNDYLNNYFMP  Y T  +++P++YA  L    + QL
Sbjct: 149 NILGDQNEASNYLSKCIYSIGLGSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQL 208

Query: 214 KV-PEFGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNI 273
           +V    GARK A+ GVG+IGC+P   A     G  C ++INSA ++FN+ L S+V   N 
Sbjct: 209 RVLYTNGARKFALIGVGAIGCSPNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQ 268

Query: 274 NFPAAKFTFID---VFKISTVNPSNYGKMVLDAPCCEV--EVGGMQCAPFGKVCENRRNY 333
           N P AKFT+I+   +F+    NP+ YG  V +A CC V    G + C P    C NR  Y
Sbjct: 269 NTPDAKFTYINAYGIFQDIITNPARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEY 328

Query: 334 MFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
           +FWD  HP E    ++  R+F  +   D +P DI  L  L
Sbjct: 329 VFWDAFHPGEAANIVIGRRSFKREAASDAHPYDIQQLASL 362

BLAST of HG10005991 vs. ExPASy Swiss-Prot
Match: Q8L5Z1 (GDSL esterase/lipase At1g33811 OS=Arabidopsis thaliana OX=3702 GN=At1g33811 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.2e-74
Identity = 153/347 (44.09%), Postives = 209/347 (60.23%), Query Frame = 0

Query: 32  VRCYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIA 91
           V C F+FGDS  DNGNNN +L      ARA+Y PYGIDF +    TGRFTNGR   D +A
Sbjct: 32  VPCLFIFGDSLVDNGNNNRLLSL----ARANYRPYGIDFPQG--TTGRFTNGRTYVDALA 91

Query: 92  KFLGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQ 151
           + LGF +YIPP+   + + IL+G N+ASG AGI  ETG  LG  +S+ +Q+E +   + Q
Sbjct: 92  QILGFRNYIPPYSRIRGQAILRGANFASGAAGIRDETGDNLGAHTSMNQQVELYTTAVQQ 151

Query: 152 M-HGLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLS 211
           M     G   +++RYL RC++   +GSNDYLNNYFMP  Y TST Y  + +A +L K  +
Sbjct: 152 MLRYFRGDTNELQRYLSRCIFYSGMGSNDYLNNYFMPDFYSTSTNYNDKTFAESLIKNYT 211

Query: 212 RQL-KVPEFGARKVAIFGVGSIGCTPYARANFEHKGFP---CVDKINSAVQLFNTGLKSL 271
           +QL ++ +FGARKV + GVG IGC PY  A + ++      C +KIN+A+ +FNT +K L
Sbjct: 212 QQLTRLYQFGARKVIVTGVGQIGCIPYQLARYNNRNNSTGRCNEKINNAIVVFNTQVKKL 271

Query: 272 VQQLNI-NFPAAKFTFIDVFKIS---TVNPSNYGKMVLDAPCCEV--EVGGMQCAPFGKV 331
           V +LN      AKF ++D +K +    VN + YG  V+D  CC V    G + C P    
Sbjct: 272 VDRLNKGQLKGAKFVYLDSYKSTYDLAVNGAAYGFEVVDKGCCGVGRNNGQITCLPLQTP 331

Query: 332 CENRRNYMFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
           C +R  Y+FWD  HPTE    L+A   F ++    TYP +I  L +L
Sbjct: 332 CPDRTKYLFWDAFHPTETANILLAKSNFYSR--AYTYPINIQELANL 370

BLAST of HG10005991 vs. ExPASy TrEMBL
Match: A0A0A0LKR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G093840 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 6.8e-167
Identity = 290/385 (75.32%), Postives = 320/385 (83.12%), Query Frame = 0

Query: 1   MKGQMM---VYCLIIIGGLIESKWVEGASTKKK----------LVRCYFVFGDSQADNGN 60
           MKG+++    YC +++  +I       AST  K          +  CYFVFGDSQADNGN
Sbjct: 1   MKGELLKVYYYCAVVVVVIINGVVDGAASTSSKHKGKGGSGRGICSCYFVFGDSQADNGN 60

Query: 61  NNDILEREYGNARADYEPYGIDFSKQR---IPTGRFTNGRNVPDFIAKFLGFHDYIPPFK 120
           NND+LEREYG ARADY+PYGIDFS      IPTGRFTN RNVPDFIAKFLGF DYIPPF+
Sbjct: 61  NNDMLEREYGRARADYKPYGIDFSSSSSSYIPTGRFTNARNVPDFIAKFLGFDDYIPPFR 120

Query: 121 TTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIRR 180
           TTKSRTILKG NYASGGAGIL ETGRTLG+VSSIKKQLENHNYTISQMH LLGS  KI R
Sbjct: 121 TTKSRTILKGANYASGGAGILRETGRTLGQVSSIKKQLENHNYTISQMHTLLGSSSKINR 180

Query: 181 YLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLK-VPEFGARKV 240
           YLK CLYTVQIGSNDYLNNYFMPK+YMTST+Y+PQA+A ALNK LS+ LK +  +GARKV
Sbjct: 181 YLKECLYTVQIGSNDYLNNYFMPKIYMTSTQYSPQAFATALNKNLSQHLKNLYNYGARKV 240

Query: 241 AIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFID 300
           AIFGVGSIGCTPYAR NFEH G PCVD+INSA+QLFN+GLKSLVQ LN N P+AKFTFID
Sbjct: 241 AIFGVGSIGCTPYARENFEHTGLPCVDEINSAIQLFNSGLKSLVQHLNANLPSAKFTFID 300

Query: 301 VFKISTVNPSNYGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTEIGFRLV 360
           VF+ISTV+P NYGKMVLDAPCCEV  G MQC+PFGKVC+NR +YMFWDGVHPTE GF+LV
Sbjct: 301 VFQISTVDPLNYGKMVLDAPCCEVGAGAMQCSPFGKVCKNRGDYMFWDGVHPTESGFKLV 360

Query: 361 ASRAFNAKQPGDTYPCDINHLRHLS 369
           ASRAFNAKQPG+ YP DINHL HLS
Sbjct: 361 ASRAFNAKQPGEAYPFDINHLVHLS 385

BLAST of HG10005991 vs. ExPASy TrEMBL
Match: A0A5D3DKM8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold419G00010 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 9.5e-161
Identity = 286/386 (74.09%), Postives = 318/386 (82.38%), Query Frame = 0

Query: 1   MKGQMM---VYCLIIIGGLIESKWVEGA---STKKK------LVRCYFVFGDSQADNGNN 60
           MKG+++    YC  ++  +I +  V+GA   ++KKK         CYFVFGDSQADNGNN
Sbjct: 1   MKGELLKGYYYCTAVVVVVIINGVVDGAASLASKKKGGGGSDACSCYFVFGDSQADNGNN 60

Query: 61  NDILEREYGNARADYEPYGIDF-----SKQRIPTGRFTNGRNVPDFIAKFLGFHDYIPPF 120
           ND+LEREYG ARADY+PYGIDF     S   IPTGRFTNGRNVPDFIA+FLGF DYIPPF
Sbjct: 61  NDMLEREYGRARADYKPYGIDFPPSSSSSSFIPTGRFTNGRNVPDFIAQFLGFRDYIPPF 120

Query: 121 KTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIR 180
           +TTKS+TILKG NYASGGAGIL ETGRTLG VSSIKKQLENHNYTISQM  L GS  K++
Sbjct: 121 RTTKSQTILKGANYASGGAGILLETGRTLGEVSSIKKQLENHNYTISQMQRLFGSSSKLK 180

Query: 181 RYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLKV-PEFGARK 240
            YLK CLYTVQIGSNDYLNNYFMP +YMTST+Y+PQA+A ALN+ LSRQLK+  E GARK
Sbjct: 181 SYLKECLYTVQIGSNDYLNNYFMPTIYMTSTQYSPQAFATALNEDLSRQLKILYEQGARK 240

Query: 241 VAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFI 300
           VAIFGVGSIGCTPYARANFEH G PCVDKIN+A+QLFN GLKSLV+ LN N P+AKFTFI
Sbjct: 241 VAIFGVGSIGCTPYARANFEHIGLPCVDKINTAIQLFNAGLKSLVEHLNANLPSAKFTFI 300

Query: 301 DVFKISTVNPSNYGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTEIGFRL 360
           DVFKISTV+P NYGK+ LDAPCCEV  G MQC PFGKVCENRR+YMF+DGVHPTE GF L
Sbjct: 301 DVFKISTVDPLNYGKVNLDAPCCEVGAGEMQCTPFGKVCENRRDYMFFDGVHPTENGFEL 360

Query: 361 VASRAFNAKQPGDTYPCDINHLRHLS 369
           VAS AFNAKQP + YP DINHL HLS
Sbjct: 361 VASSAFNAKQPDEAYPFDINHLVHLS 386

BLAST of HG10005991 vs. ExPASy TrEMBL
Match: A0A1S3B473 (GDSL esterase/lipase At1g29670-like OS=Cucumis melo OX=3656 GN=LOC103485821 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 9.5e-161
Identity = 286/386 (74.09%), Postives = 318/386 (82.38%), Query Frame = 0

Query: 1   MKGQMM---VYCLIIIGGLIESKWVEGA---STKKK------LVRCYFVFGDSQADNGNN 60
           MKG+++    YC  ++  +I +  V+GA   ++KKK         CYFVFGDSQADNGNN
Sbjct: 1   MKGELLKGYYYCTAVVVVVIINGVVDGAASLASKKKGGGGSDACSCYFVFGDSQADNGNN 60

Query: 61  NDILEREYGNARADYEPYGIDF-----SKQRIPTGRFTNGRNVPDFIAKFLGFHDYIPPF 120
           ND+LEREYG ARADY+PYGIDF     S   IPTGRFTNGRNVPDFIA+FLGF DYIPPF
Sbjct: 61  NDMLEREYGRARADYKPYGIDFPPSSSSSSFIPTGRFTNGRNVPDFIAQFLGFRDYIPPF 120

Query: 121 KTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIR 180
           +TTKS+TILKG NYASGGAGIL ETGRTLG VSSIKKQLENHNYTISQM  L GS  K++
Sbjct: 121 RTTKSQTILKGANYASGGAGILLETGRTLGEVSSIKKQLENHNYTISQMQRLFGSSSKLK 180

Query: 181 RYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLKV-PEFGARK 240
            YLK CLYTVQIGSNDYLNNYFMP +YMTST+Y+PQA+A ALN+ LSRQLK+  E GARK
Sbjct: 181 SYLKECLYTVQIGSNDYLNNYFMPTIYMTSTQYSPQAFATALNEDLSRQLKILYEQGARK 240

Query: 241 VAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFI 300
           VAIFGVGSIGCTPYARANFEH G PCVDKIN+A+QLFN GLKSLV+ LN N P+AKFTFI
Sbjct: 241 VAIFGVGSIGCTPYARANFEHIGLPCVDKINTAIQLFNAGLKSLVEHLNANLPSAKFTFI 300

Query: 301 DVFKISTVNPSNYGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTEIGFRL 360
           DVFKISTV+P NYGK+ LDAPCCEV  G MQC PFGKVCENRR+YMF+DGVHPTE GF L
Sbjct: 301 DVFKISTVDPLNYGKVNLDAPCCEVGAGEMQCTPFGKVCENRRDYMFFDGVHPTENGFEL 360

Query: 361 VASRAFNAKQPGDTYPCDINHLRHLS 369
           VAS AFNAKQP + YP DINHL HLS
Sbjct: 361 VASSAFNAKQPDEAYPFDINHLVHLS 386

BLAST of HG10005991 vs. ExPASy TrEMBL
Match: A0A6J1IEL6 (GDSL esterase/lipase At1g29670-like OS=Cucurbita maxima OX=3661 GN=LOC111472555 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.5e-134
Identity = 240/380 (63.16%), Postives = 277/380 (72.89%), Query Frame = 0

Query: 1   MKGQMMVYCLIIIGGLIESKWVEGASTK---------------KKLVRCYFVFGDSQADN 60
           MKGQ +V   ++      S WV+GAS +               K  V CYFV GDSQADN
Sbjct: 1   MKGQSLVVLCLLTTACFASSWVDGASPRIKSRTRPKSNATTQTKTKVGCYFVLGDSQADN 60

Query: 61  GNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKFLGFHDYIPPFKT 120
           GNNN ++E  YGNARADY+PYG DF+++  PTGRFTNGRN PDFIA +LGF  YI PF T
Sbjct: 61  GNNN-VMEMAYGNARADYKPYGCDFNREGTPTGRFTNGRNAPDFIATYLGFRHYISPFTT 120

Query: 121 TKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIRRY 180
            K R IL GVNYASGGAGIL ETGRTLG+V SI KQLENHN TISQ+ GLLG+      +
Sbjct: 121 IKGREILDGVNYASGGAGILPETGRTLGQVLSIHKQLENHNITISQIRGLLGTRSATTTH 180

Query: 181 LKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQLKV-PEFGARKVA 240
           L  CLYTVQIGSNDYLNNYFMP+ Y TS ++TPQ +A  LN  L  +LKV  E GARKVA
Sbjct: 181 LNSCLYTVQIGSNDYLNNYFMPQFYKTSAQFTPQQFATVLNNRLYTELKVLYEQGARKVA 240

Query: 241 IFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFIDV 300
           +FGVGSIGCTPYARANFE+KG  CVDKIN+A+QLFN GLK+LV QLN     AKFT+IDV
Sbjct: 241 LFGVGSIGCTPYARANFENKGGGCVDKINNAIQLFNDGLKALVLQLNTKMAGAKFTYIDV 300

Query: 301 FKISTVNPSNYGKMVLDAPCCEVEVGGMQCAPFGKVCENRRNYMFWDGVHPTEIGFRLVA 360
           FKIS+  PS  G+MVL+APCCEV+ G +QC   G++C NR  YMFWD VHPTE+G   +A
Sbjct: 301 FKISSTIPSTSGRMVLNAPCCEVDAGQVQCTRLGRICGNRMEYMFWDAVHPTEVGISALA 360

Query: 361 SRAFNAKQPGDTYPCDINHL 365
           SRAF AK+ GD YP DIN L
Sbjct: 361 SRAFKAKEAGDAYPYDINQL 379

BLAST of HG10005991 vs. ExPASy TrEMBL
Match: A0A6J1CSH4 (GDSL esterase/lipase At1g29670-like OS=Momordica charantia OX=3673 GN=LOC111014175 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 9.9e-134
Identity = 233/348 (66.95%), Postives = 273/348 (78.45%), Query Frame = 0

Query: 26  STKKKLVRCYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQR--IPTGRFTNG 85
           S  K  + CYF+FGDSQ+DNGNNNDI     G+A A+Y+PYGIDF  Q    PTGRFTNG
Sbjct: 52  SKSKSRMDCYFIFGDSQSDNGNNNDI----KGDAMANYKPYGIDFPNQNSPTPTGRFTNG 111

Query: 86  RNVPDFIAKFLGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLE 145
           RN+PDFIA+ LGFHDYIPPF+ T    +LKGVNYASGG+GI  ETGR LG+V SI KQLE
Sbjct: 112 RNLPDFIAELLGFHDYIPPFRNTTGWNVLKGVNYASGGSGIHSETGRMLGKVLSINKQLE 171

Query: 146 NHNYTISQMHGLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAI 205
           NHN+TIS++ G LGS++   ++LK CLYTVQ GSNDYLNNYF+P  Y TS ++TPQ YA+
Sbjct: 172 NHNHTISRIQGSLGSKLAATKHLKSCLYTVQAGSNDYLNNYFLPFSYKTSAQFTPQQYAV 231

Query: 206 ALNKYLSRQL-KVPEFGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTG 265
           ALNK LS QL ++ + G RKVA+FGVG IGCTPYARANFEH+G PCV+KIN A+QLFN+G
Sbjct: 232 ALNKQLSHQLEELYDRGGRKVAVFGVGGIGCTPYARANFEHEGSPCVEKINYAIQLFNSG 291

Query: 266 LKSLVQQLNINFPAAKFTFIDVFKISTVNPSNY---GKMVLDAPCCEVEVGGMQCAPFGK 325
           LKSL+  LN  F  AKF FIDVFKIST+ P+       +VLDAPCCEV VG +QC PFGK
Sbjct: 292 LKSLIHDLNTKFTDAKFIFIDVFKISTMKPTPLVPADLLVLDAPCCEVPVGQLQCTPFGK 351

Query: 326 VCENRRNYMFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
           VC NR  YMFWDGVHPTE+GF+LVA RAFNAK+P DTYP DI+HL  L
Sbjct: 352 VCGNRSKYMFWDGVHPTELGFKLVAKRAFNAKEPDDTYPFDISHLVRL 395

BLAST of HG10005991 vs. TAIR 10
Match: AT1G29670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 1.1e-87
Identity = 165/342 (48.25%), Postives = 216/342 (63.16%), Query Frame = 0

Query: 32  VRCYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIA 91
           V C+FVFGDS  DNGNNN ++      AR++Y PYGIDF     PTGRF+NG+   D IA
Sbjct: 30  VPCFFVFGDSLVDNGNNNGLI----SIARSNYFPYGIDFGG---PTGRFSNGKTTVDVIA 89

Query: 92  KFLGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQ 151
           + LGF+ YIP + T   R IL GVNYAS  AGI  ETGR LG+  S   Q+ N+  T+SQ
Sbjct: 90  ELLGFNGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQRISFSGQVRNYQTTVSQ 149

Query: 152 MHGLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSR 211
           +  LLG E +   YLKRC+Y+V +GSNDYLNNYFMP  Y +S ++TP+ YA  L    S 
Sbjct: 150 VVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQFTPEQYANDLISRYST 209

Query: 212 QLK-VPEFGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQL 271
           QL  +  +GARK A+ G+G++GC+P A A     G  CVD+INSA Q+FN  L+SLV QL
Sbjct: 210 QLNALYNYGARKFALSGIGAVGCSPNALAG-SPDGRTCVDRINSANQIFNNKLRSLVDQL 269

Query: 272 NINFPAAKFTFID---VFKISTVNPSNYGKMVLDAPCCEV--EVGGMQCAPFGKVCENRR 331
           N N P AKF +I+   +F+    NP+ +G  V +A CC +    G + C P  + C +R 
Sbjct: 270 NNNHPDAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIGRNAGQITCLPGQRPCRDRN 329

Query: 332 NYMFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
            Y+FWD  HPTE    ++A R++NA+   D YP DI+ L  L
Sbjct: 330 AYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDISRLAQL 363

BLAST of HG10005991 vs. TAIR 10
Match: AT1G29660.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 5.4e-84
Identity = 169/378 (44.71%), Postives = 222/378 (58.73%), Query Frame = 0

Query: 1   MKGQMMVYCLIIIGGLIESKWV--EGASTKKKL---VRCYFVFGDSQADNGNNNDILERE 60
           M+  +  +CL+       S WV   G   K K    V CYF+FGDS  DNGNNN    R 
Sbjct: 1   MESYLRKWCLV-------SVWVLLLGLGFKVKAEPQVPCYFIFGDSLVDNGNNN----RL 60

Query: 61  YGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKFLGFHDYIPPFKTTKSRTILKGV 120
              ARADY PYGIDF     PTGRF+NGR   D + + LGF +YIP + T   + IL+GV
Sbjct: 61  RSIARADYFPYGIDFGG---PTGRFSNGRTTVDVLTELLGFDNYIPAYSTVSGQEILQGV 120

Query: 121 NYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMHGLLGSEIKIRRYLKRCLYTVQI 180
           NYAS  AGI  ETG  LG+  +   Q+EN+  T++Q+  +LG E     YLKRC+Y+V +
Sbjct: 121 NYASAAAGIREETGAQLGQRITFSGQVENYKNTVAQVVEILGDEYTAADYLKRCIYSVGM 180

Query: 181 GSNDYLNNYFMPKLYMTSTRYTPQAYAIAL-NKYLSRQLKVPEFGARKVAIFGVGSIGCT 240
           GSNDYLNNYFMP+ Y TS +YTP+ YA  L ++Y  +   +  +GARK A+ G+G+IGC+
Sbjct: 181 GSNDYLNNYFMPQFYSTSRQYTPEQYADDLISRYRDQLNALYNYGARKFALVGIGAIGCS 240

Query: 241 PYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNINFPAAKFTFID---VFKISTVN 300
           P A A     G  CV++INSA ++FN  L S+VQQLN     A FT+I+    F+    N
Sbjct: 241 PNALAQGSQDGTTCVERINSANRIFNNRLISMVQQLNNAHSDASFTYINAYGAFQDIIAN 300

Query: 301 PSNYGKMVLDAPCCEVEVGGMQ--CAPFGKVCENRRNYMFWDGVHPTEIGFRLVASRAFN 360
           PS YG    +  CC +   G Q  C P    C NR  Y+FWD  HP+      +A R++N
Sbjct: 301 PSAYGFTNTNTACCGIGRNGGQLTCLPGEPPCLNRDEYVFWDAFHPSAAANTAIAKRSYN 360

Query: 361 AKQPGDTYPCDINHLRHL 368
           A++  D YP DI+ L  L
Sbjct: 361 AQRSSDVYPIDISQLAQL 364

BLAST of HG10005991 vs. TAIR 10
Match: AT4G18970.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 6.6e-82
Identity = 157/340 (46.18%), Postives = 204/340 (60.00%), Query Frame = 0

Query: 34  CYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKF 93
           CYF+FGDS  D+GNNN    R    ARA+Y PYGIDF  Q  PTGRF+NG+   D I + 
Sbjct: 28  CYFIFGDSLVDSGNNN----RLTSLARANYFPYGIDF--QYGPTGRFSNGKTTVDVITEL 87

Query: 94  LGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMH 153
           LGF DYI P+   +   IL+GVNYAS  AGI  ETGR LG   +   Q+ NH  T+SQ+ 
Sbjct: 88  LGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVSQVV 147

Query: 154 GLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQL 213
            +LG E +   YL +C+Y++ +GSNDYLNNYFMP  Y T ++Y+P AYA  L    + QL
Sbjct: 148 NILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYTEQL 207

Query: 214 KVP-EFGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNI 273
           ++    GARK A+ G+G+IGC+P   A     G  C ++INSA ++FN+ L SLV   N 
Sbjct: 208 RIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDHFNQ 267

Query: 274 NFPAAKFTFID---VFKISTVNPSNYGKMVLDAPCCEV--EVGGMQCAPFGKVCENRRNY 333
           N P AKFT+I+   +F+    NPS YG  V +A CC V    G + C P    C NR  Y
Sbjct: 268 NTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRDEY 327

Query: 334 MFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
           +FWD  HP E    ++ SR+F  +   D +P DI  L  L
Sbjct: 328 VFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQLARL 361

BLAST of HG10005991 vs. TAIR 10
Match: AT4G18970.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 6.6e-82
Identity = 157/340 (46.18%), Postives = 204/340 (60.00%), Query Frame = 0

Query: 34  CYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKF 93
           CYF+FGDS  D+GNNN    R    ARA+Y PYGIDF  Q  PTGRF+NG+   D I + 
Sbjct: 28  CYFIFGDSLVDSGNNN----RLTSLARANYFPYGIDF--QYGPTGRFSNGKTTVDVITEL 87

Query: 94  LGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMH 153
           LGF DYI P+   +   IL+GVNYAS  AGI  ETGR LG   +   Q+ NH  T+SQ+ 
Sbjct: 88  LGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVSQVV 147

Query: 154 GLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQL 213
            +LG E +   YL +C+Y++ +GSNDYLNNYFMP  Y T ++Y+P AYA  L    + QL
Sbjct: 148 NILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYTEQL 207

Query: 214 KVP-EFGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNI 273
           ++    GARK A+ G+G+IGC+P   A     G  C ++INSA ++FN+ L SLV   N 
Sbjct: 208 RIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDHFNQ 267

Query: 274 NFPAAKFTFID---VFKISTVNPSNYGKMVLDAPCCEV--EVGGMQCAPFGKVCENRRNY 333
           N P AKFT+I+   +F+    NPS YG  V +A CC V    G + C P    C NR  Y
Sbjct: 268 NTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRDEY 327

Query: 334 MFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
           +FWD  HP E    ++ SR+F  +   D +P DI  L  L
Sbjct: 328 VFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQLARL 361

BLAST of HG10005991 vs. TAIR 10
Match: AT5G45670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 1.1e-79
Identity = 153/340 (45.00%), Postives = 205/340 (60.29%), Query Frame = 0

Query: 34  CYFVFGDSQADNGNNNDILEREYGNARADYEPYGIDFSKQRIPTGRFTNGRNVPDFIAKF 93
           CYF+FGDS  DNGNNN +       ARA+Y PYGIDF+    PTGRF+NG    D IA+ 
Sbjct: 29  CYFIFGDSLVDNGNNNQL----QSLARANYFPYGIDFAAG--PTGRFSNGLTTVDVIAQL 88

Query: 94  LGFHDYIPPFKTTKSRTILKGVNYASGGAGILHETGRTLGRVSSIKKQLENHNYTISQMH 153
           LGF DYI P+ + + + IL+GVNYAS  AGI  ETGR LG   +   Q+ NH  T+SQ+ 
Sbjct: 89  LGFEDYITPYASARGQDILRGVNYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVV 148

Query: 154 GLLGSEIKIRRYLKRCLYTVQIGSNDYLNNYFMPKLYMTSTRYTPQAYAIALNKYLSRQL 213
            +LG + +   YL +C+Y++ +GSNDYLNNYFMP  Y T  +++P++YA  L    + QL
Sbjct: 149 NILGDQNEASNYLSKCIYSIGLGSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQL 208

Query: 214 KV-PEFGARKVAIFGVGSIGCTPYARANFEHKGFPCVDKINSAVQLFNTGLKSLVQQLNI 273
           +V    GARK A+ GVG+IGC+P   A     G  C ++INSA ++FN+ L S+V   N 
Sbjct: 209 RVLYTNGARKFALIGVGAIGCSPNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQ 268

Query: 274 NFPAAKFTFID---VFKISTVNPSNYGKMVLDAPCCEV--EVGGMQCAPFGKVCENRRNY 333
           N P AKFT+I+   +F+    NP+ YG  V +A CC V    G + C P    C NR  Y
Sbjct: 269 NTPDAKFTYINAYGIFQDIITNPARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEY 328

Query: 334 MFWDGVHPTEIGFRLVASRAFNAKQPGDTYPCDINHLRHL 368
           +FWD  HP E    ++  R+F  +   D +P DI  L  L
Sbjct: 329 VFWDAFHPGEAANIVIGRRSFKREAASDAHPYDIQQLASL 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891276.18.5e-18084.05GDSL esterase/lipase At1g29670-like [Benincasa hispida][more]
XP_011649033.11.4e-16675.32GDSL esterase/lipase At1g29670 [Cucumis sativus] >XP_031737227.1 GDSL esterase/l... [more]
XP_008441762.12.0e-16074.09PREDICTED: GDSL esterase/lipase At1g29670-like [Cucumis melo] >KAA0051383.1 GDSL... [more]
KAG6598965.12.4e-13461.54GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022973923.13.1e-13463.16GDSL esterase/lipase At1g29670-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9C7N41.5e-8648.25GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2... [more]
Q9C7N57.6e-8344.71GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1... [more]
Q93YW89.3e-8146.18GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2... [more]
Q9FK751.5e-7845.00GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2... [more]
Q8L5Z12.2e-7444.09GDSL esterase/lipase At1g33811 OS=Arabidopsis thaliana OX=3702 GN=At1g33811 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0LKR66.8e-16775.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G093840 PE=3 SV=1[more]
A0A5D3DKM89.5e-16174.09GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold419... [more]
A0A1S3B4739.5e-16174.09GDSL esterase/lipase At1g29670-like OS=Cucumis melo OX=3656 GN=LOC103485821 PE=3... [more]
A0A6J1IEL61.5e-13463.16GDSL esterase/lipase At1g29670-like OS=Cucurbita maxima OX=3661 GN=LOC111472555 ... [more]
A0A6J1CSH49.9e-13466.95GDSL esterase/lipase At1g29670-like OS=Momordica charantia OX=3673 GN=LOC1110141... [more]
Match NameE-valueIdentityDescription
AT1G29670.11.1e-8748.25GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G29660.15.4e-8444.71GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT4G18970.16.6e-8246.18GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT4G18970.26.6e-8246.18GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G45670.11.1e-7945.00GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 23..353
e-value: 9.1E-67
score: 227.7
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 35..345
e-value: 1.3E-29
score: 104.3
NoneNo IPR availablePANTHERPTHR45650GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATEDcoord: 7..365
NoneNo IPR availablePANTHERPTHR45650:SF62SUBFAMILY NOT NAMEDcoord: 7..365
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 38..348
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 34..350
e-value: 2.76624E-108
score: 318.02

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10005991.1HG10005991.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds