HG10005852 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10005852
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr07: 7047677 .. 7048126 (+)
RNA-Seq Expression	HG10005852
Synteny	HG10005852

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGCCCTTGAGGAGTTCGATCAGACTTTCATCCAACGCCCAGAGCACCGCCTCAAGCTTGCCATCTCCGAAGCTGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACTCCCCCGGCTGCAATTTTCCCAACGATGTGGTCCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTCTGGTGGTCAATCACGGCGTCCCTCCCGAGAAGCAGCGCCGGATAGAGGTCGCTGCCCGAGAGTTTTTCGGGCAGAGTTTGGAAGAGAAGAGGAAAGTGAGGAGAAACGAAGGGGTGGTGACTGGCTATTTTGATACGGAGCTGACCAAGAACGTAAGAGATTGGAAGGAGGTGTTTGATTTCATGGTGGCGAATCATCCTGATGACGACAAGTTGATTCATGTAGGTCTCACAGTCACTGTCCAATTGTTTAAACTTTAG

mRNA sequence

ATGGGAGCCCTTGAGGAGTTCGATCAGACTTTCATCCAACGCCCAGAGCACCGCCTCAAGCTTGCCATCTCCGAAGCTGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACTCCCCCGGCTGCAATTTTCCCAACGATGTGGTCCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTCTGGTGGTCAATCACGGCGTCCCTCCCGAGAAGCAGCGCCGGATAGAGGTCGCTGCCCGAGAGTTTTTCGGGCAGAGTTTGGAAGAGAAGAGGAAAGTGAGGAGAAACGAAGGGGTGGTGACTGGCTATTTTGATACGGAGCTGACCAAGAACGTAAGAGATTGGAAGGAGGTGTTTGATTTCATGGTGGCGAATCATCCTGATGACGACAAGTTGATTCATGTAGGTCTCACAGTCACTGTCCAATTGTTTAAACTTTAG

Coding sequence (CDS)

ATGGGAGCCCTTGAGGAGTTCGATCAGACTTTCATCCAACGCCCAGAGCACCGCCTCAAGCTTGCCATCTCCGAAGCTGACGGAATCCCCACCATCGACCTCTCTCCGATCTTCAACTCCCCCGGCTGCAATTTTCCCAACGATGTGGTCCGACAAATCGCCTCTGCTTCCAAGGAGTGGGGATTCTTTCTGGTGGTCAATCACGGCGTCCCTCCCGAGAAGCAGCGCCGGATAGAGGTCGCTGCCCGAGAGTTTTTCGGGCAGAGTTTGGAAGAGAAGAGGAAAGTGAGGAGAAACGAAGGGGTGGTGACTGGCTATTTTGATACGGAGCTGACCAAGAACGTAAGAGATTGGAAGGAGGTGTTTGATTTCATGGTGGCGAATCATCCTGATGACGACAAGTTGATTCATGTAGGTCTCACAGTCACTGTCCAATTGTTTAAACTTTAG

Protein sequence

MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWKEVFDFMVANHPDDDKLIHVGLTVTVQLFKL

Homology

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BLAST of HG10005852 vs. NCBI nr
Match: XP_038887395.1 (protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida])

HSP 1 Score: 240.4 bits (612), Expect = 1.0e-59
Identity = 117/145 (80.69%), Postives = 127/145 (87.59%), Query Frame = 0


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEW 60
           MGALE+FD+ FIQ PEHR KLA+SEADGIPTIDLSPIFNSPG +FP+D+VRQIASAS EW
Sbjct: 1   MGALEDFDKAFIQAPEHRPKLAVSEADGIPTIDLSPIFNSPGGDFPDDLVRQIASASSEW 60

Query: 61  GFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWKE 120
           GFFLVVNHGVP EK+RRIE  AREFFGQSLEEKRKVRR+EGVVTGYFDTELTKNVRDWKE
Sbjct: 61  GFFLVVNHGVPSEKRRRIEATAREFFGQSLEEKRKVRRDEGVVTGYFDTELTKNVRDWKE 120

Query: 121 VFDFMVAN--------HPDDDKLIH 138
           VFDFMVA+         PDDD+L H
Sbjct: 121 VFDFMVADPTVVPASPEPDDDELTH 145

[Close]

BLAST of HG10005852 vs. NCBI nr
Match: KAG7011675.1 (putative 2-oxoglutarate-dependent dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 223.0 bits (567), Expect = 1.7e-54
Identity = 108/145 (74.48%), Postives = 123/145 (84.83%), Query Frame = 0


Query: 4   LEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFF 63
           +++FDQ FIQ PEHR KL+  EADGIPT+DLSPIFNSP   FP+D+VRQIASAS +WGFF
Sbjct: 1   MDDFDQAFIQAPEHRPKLSDPEADGIPTVDLSPIFNSPDGTFPDDLVRQIASASADWGFF 60

Query: 64  LVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWKEVFD 123
            VVNHGVPPEKQRRIE AAREFFGQSLEEKRKVRR+EGVVTGY +TELTKNVRDWKEVFD
Sbjct: 61  FVVNHGVPPEKQRRIEAAAREFFGQSLEEKRKVRRDEGVVTGYSNTELTKNVRDWKEVFD 120

Query: 124 FMVAN--------HPDDDKLIHVGL 141
           F+VA+         PDDD+L ++GL
Sbjct: 121 FVVADPTVVPASPEPDDDQLTNLGL 145

[Close]

BLAST of HG10005852 vs. NCBI nr
Match: KAA0031270.1 (protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 220.7 bits (561), Expect = 8.4e-54
Identity = 110/144 (76.39%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LE+FD  FIQ PEHR KLA+S+ADGIPTIDLSPIFNS PG +FP+ +V+QIASA  E
Sbjct: 1   MGDLEDFDHAFIQAPEHRPKLALSDADGIPTIDLSPIFNSVPGTDFPHHLVQQIASACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+RRIE AAREFFGQSLEEKRKVRRNE VVTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRRRIEAAAREFFGQSLEEKRKVRRNEWVVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMV--------ANHPDDDKL 136
           EVFDF+V        ++ PDD +L
Sbjct: 121 EVFDFVVEDPTVIPASSDPDDTEL 144

[Close]

BLAST of HG10005852 vs. NCBI nr
Match: XP_008454901.1 (PREDICTED: protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo] >TYK06722.1 protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 220.7 bits (561), Expect = 8.4e-54
Identity = 110/144 (76.39%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LE+FD  FIQ PEHR KLA+S+ADGIPTIDLSPIFNS PG +FP+ +V+QIASA  E
Sbjct: 1   MGDLEDFDHAFIQAPEHRPKLALSDADGIPTIDLSPIFNSVPGTDFPHHLVQQIASACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+RRIE AAREFFGQSLEEKRKVRRNE VVTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRRRIEAAAREFFGQSLEEKRKVRRNEWVVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMV--------ANHPDDDKL 136
           EVFDF+V        ++ PDD +L
Sbjct: 121 EVFDFVVEDPTVIPASSDPDDTEL 144

[Close]

BLAST of HG10005852 vs. NCBI nr
Match: KAA0031269.1 (protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo var. makuwa] >TYK06721.1 protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 220.3 bits (560), Expect = 1.1e-53
Identity = 108/144 (75.00%), Postives = 123/144 (85.42%), Query Frame = 0


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LEEFDQ F+Q PEHR KLA+S+AD IPTIDLSPIFNS PG +FP+D+V+QIA+A  E
Sbjct: 1   MGDLEEFDQAFVQSPEHRPKLALSDADEIPTIDLSPIFNSVPGSDFPDDLVQQIAAACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+ RIE AAREFFGQSLEEKRKVRR+EG+VTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGMVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMVAN--------HPDDDKL 136
           EVFD +VA+         PDD++L
Sbjct: 121 EVFDLVVADPTIVPASPEPDDEEL 144

[Close]

BLAST of HG10005852 vs. ExPASy Swiss-Prot
Match: A0A023GS29 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase Diox2 OS=Ruta graveolens OX=37565 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.3e-09
Identity = 39/104 (37.50%), Postives = 54/104 (51.92%), Query Frame = 0


Query: 23  ISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAA 82
           I E   IP ID+S  ++SP      +VV+ I  A++ WGFF +VNHGVP E   R++ A 
Sbjct: 59  ILERASIPLIDMSE-WDSP------EVVKSICDAAENWGFFQIVNHGVPLETLERVKEAT 118

Query: 83  REFFGQSLEEKRKV-RRNEGVVTGYFDTELTKNVRDWKEVFDFM 126
             FFG   EEK K  + N  +    F +    +V    E  DF+
Sbjct: 119 HRFFGLPAEEKNKYSKENSPINNVRFGSSFVPHVEKALEWKDFL 155

[Close]

BLAST of HG10005852 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.9e-09
Identity = 35/96 (36.46%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 29  IPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAAREFFGQ 88
           IP +D++ ++  P      + +R + SA +EWGFF +VNHGV      R+  A REFF  
Sbjct: 48  IPVLDMNDVWGKP------EGLRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFEL 107

Query: 89  SLEEKRKVRRNEGVVTGYFD-TELTKNVR-DWKEVF 123
            LEEKRK   +     GY     + K+ + DW + F
Sbjct: 108 PLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYF 137

[Close]

BLAST of HG10005852 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 6.6e-09
Identity = 38/137 (27.74%), Postives = 65/137 (47.45%), Query Frame = 0


Query: 4   LEEFDQTFIQRPEHRL--KLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWG 63
           ++   + +IQ  E RL  K      + IP ID+S    +P     + V   +  A+++WG
Sbjct: 35  IKALPEQYIQPLEERLINKFVNETDEAIPVIDMS----NPD---EDRVAEAVCDAAEKWG 94

Query: 64  FFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVT----GYFDTELTKNVRD 123
           FF V+NHGVP E    ++ A  +FF   +EEKRK  +   + T    G   + L +   +
Sbjct: 95  FFQVINHGVPLEVLDDVKAATHKFFNLPVEEKRKFTKENSLSTTVRFGTSFSPLAEQALE 154

Query: 124 WKEVFDFMVANHPDDDK 135
           WK+       +  + ++
Sbjct: 155 WKDYLSLFFVSEAEAEQ 164

[Close]

BLAST of HG10005852 vs. ExPASy Swiss-Prot
Match: O04395 (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana OX=3724 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 6.6e-09
Identity = 32/78 (41.03%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 29  IPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAAREFFGQ 88
           +P +DLS       C     V R +  AS++WG F VVNHG+P E  +R++   REFF  
Sbjct: 2   VPVVDLS-------CPDEELVARTVVKASEDWGVFQVVNHGIPTELIQRLQKVGREFFEL 61

Query: 89  SLEEKRKVRRNEGVVTGY 107
              EKR   R  G V GY
Sbjct: 62  PEAEKRSCAREAGSVEGY 72

[Close]

BLAST of HG10005852 vs. ExPASy Swiss-Prot
Match: A0A023GS28 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase Diox1 OS=Ruta graveolens OX=37565 GN=DIOX1 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 8.6e-09
Identity = 38/104 (36.54%), Postives = 53/104 (50.96%), Query Frame = 0


Query: 23  ISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWGFFLVVNHGVPPEKQRRIEVAA 82
           I E   IP ID+S  ++SP      +VV+ I  A++ WGFF +VNHGVP E   R++ A 
Sbjct: 60  ILERASIPLIDMSQ-WDSP------EVVKSICDAAENWGFFQIVNHGVPLETLERVKEAT 119

Query: 83  REFFGQSLEEKRKV-RRNEGVVTGYFDTELTKNVRDWKEVFDFM 126
             FFG   EEK    + N  +    F +    +V    E  DF+
Sbjct: 120 HRFFGLPAEEKNNYSKENSPINNVRFGSSFVPHVEKALEWKDFL 156

[Close]

BLAST of HG10005852 vs. ExPASy TrEMBL
Match: A0A5A7SKK1 (Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G00230 PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.1e-54
Identity = 110/144 (76.39%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LE+FD  FIQ PEHR KLA+S+ADGIPTIDLSPIFNS PG +FP+ +V+QIASA  E
Sbjct: 1   MGDLEDFDHAFIQAPEHRPKLALSDADGIPTIDLSPIFNSVPGTDFPHHLVQQIASACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+RRIE AAREFFGQSLEEKRKVRRNE VVTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRRRIEAAAREFFGQSLEEKRKVRRNEWVVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMV--------ANHPDDDKL 136
           EVFDF+V        ++ PDD +L
Sbjct: 121 EVFDFVVEDPTVIPASSDPDDTEL 144

[Close]

BLAST of HG10005852 vs. ExPASy TrEMBL
Match: A0A5D3C5Z7 (Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00230 PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.1e-54
Identity = 110/144 (76.39%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LE+FD  FIQ PEHR KLA+S+ADGIPTIDLSPIFNS PG +FP+ +V+QIASA  E
Sbjct: 1   MGDLEDFDHAFIQAPEHRPKLALSDADGIPTIDLSPIFNSVPGTDFPHHLVQQIASACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+RRIE AAREFFGQSLEEKRKVRRNE VVTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRRRIEAAAREFFGQSLEEKRKVRRNEWVVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMV--------ANHPDDDKL 136
           EVFDF+V        ++ PDD +L
Sbjct: 121 EVFDFVVEDPTVIPASSDPDDTEL 144

[Close]

BLAST of HG10005852 vs. ExPASy TrEMBL
Match: A0A1S3BZ66 (protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 GN=LOC103495206 PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.1e-54
Identity = 110/144 (76.39%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LE+FD  FIQ PEHR KLA+S+ADGIPTIDLSPIFNS PG +FP+ +V+QIASA  E
Sbjct: 1   MGDLEDFDHAFIQAPEHRPKLALSDADGIPTIDLSPIFNSVPGTDFPHHLVQQIASACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+RRIE AAREFFGQSLEEKRKVRRNE VVTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRRRIEAAAREFFGQSLEEKRKVRRNEWVVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMV--------ANHPDDDKL 136
           EVFDF+V        ++ PDD +L
Sbjct: 121 EVFDFVVEDPTVIPASSDPDDTEL 144

[Close]

BLAST of HG10005852 vs. ExPASy TrEMBL
Match: A0A5A7SPM0 (Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00220 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 5.3e-54
Identity = 108/144 (75.00%), Postives = 123/144 (85.42%), Query Frame = 0


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LEEFDQ F+Q PEHR KLA+S+AD IPTIDLSPIFNS PG +FP+D+V+QIA+A  E
Sbjct: 1   MGDLEEFDQAFVQSPEHRPKLALSDADEIPTIDLSPIFNSVPGSDFPDDLVQQIAAACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+ RIE AAREFFGQSLEEKRKVRR+EG+VTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGMVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMVAN--------HPDDDKL 136
           EVFD +VA+         PDD++L
Sbjct: 121 EVFDLVVADPTIVPASPEPDDEEL 144

[Close]

BLAST of HG10005852 vs. ExPASy TrEMBL
Match: A0A1S3BZR3 (LOW QUALITY PROTEIN: protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 GN=LOC103495207 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 5.3e-54
Identity = 108/144 (75.00%), Postives = 123/144 (85.42%), Query Frame = 0


Query: 1   MGALEEFDQTFIQRPEHRLKLAISEADGIPTIDLSPIFNS-PGCNFPNDVVRQIASASKE 60
           MG LEEFDQ F+Q PEHR KLA+S+AD IPTIDLSPIFNS PG +FP+D+V+QIA+A  E
Sbjct: 1   MGDLEEFDQAFVQSPEHRPKLALSDADEIPTIDLSPIFNSVPGSDFPDDLVQQIAAACTE 60

Query: 61  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 120
           WGFFLVVNHGVPPEK+ RIE AAREFFGQSLEEKRKVRR+EG+VTGYFDTELTKNVRDWK
Sbjct: 61  WGFFLVVNHGVPPEKRHRIEAAAREFFGQSLEEKRKVRRSEGMVTGYFDTELTKNVRDWK 120

Query: 121 EVFDFMVAN--------HPDDDKL 136
           EVFD +VA+         PDD++L
Sbjct: 121 EVFDLVVADPTIVPASPEPDDEEL 144

[Close]

BLAST of HG10005852 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 117.9 bits (294), Expect = 7.2e-27
Identity = 60/127 (47.24%), Postives = 80/127 (62.99%), Query Frame = 0


Query: 6   EFDQTFIQRPEHRLKLAISE------ADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKE 65
           E D+ FIQ PEHR    ++       +D IPTIDLS + ++   +    + ++IA A K 
Sbjct: 3   ELDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTH--HDKTAIAKEIAEACKR 62

Query: 66  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 125
           WGFF V+NHG+P   + R+E  A EFF  + EEKRKV+R+E    GY D E TKNVRDWK
Sbjct: 63  WGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWK 122

Query: 126 EVFDFMV 127
           E+FDF +
Sbjct: 123 EIFDFFL 127

[Close]

BLAST of HG10005852 vs. TAIR 10
Match: AT3G19000.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 117.9 bits (294), Expect = 7.2e-27
Identity = 60/127 (47.24%), Postives = 80/127 (62.99%), Query Frame = 0


Query: 6   EFDQTFIQRPEHRLKLAISE------ADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKE 65
           E D+ FIQ PEHR    ++       +D IPTIDLS + ++   +    + ++IA A K 
Sbjct: 3   ELDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTH--HDKTAIAKEIAEACKR 62

Query: 66  WGFFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWK 125
           WGFF V+NHG+P   + R+E  A EFF  + EEKRKV+R+E    GY D E TKNVRDWK
Sbjct: 63  WGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWK 122

Query: 126 EVFDFMV 127
           E+FDF +
Sbjct: 123 EIFDFFL 127

[Close]

BLAST of HG10005852 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 2.8e-23
Identity = 53/122 (43.44%), Postives = 77/122 (63.11%), Query Frame = 0


Query: 4   LEEFDQTFIQRPEHR--LKLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWG 63
           +E+ D T+IQ PEHR       ++ + IP IDLS + +        +V+ +I  A ++WG
Sbjct: 1   MEDLDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDV---QNVISEIGDACEKWG 60

Query: 64  FFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWKEV 123
           FF V+NHGVP + ++R+E   + FF   +EEK KV+R++    GY D E TKNV+DWKEV
Sbjct: 61  FFQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEV 119

[Close]

BLAST of HG10005852 vs. TAIR 10
Match: AT3G19010.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 2.8e-23
Identity = 53/122 (43.44%), Postives = 77/122 (63.11%), Query Frame = 0


Query: 4   LEEFDQTFIQRPEHR--LKLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWG 63
           +E+ D T+IQ PEHR       ++ + IP IDLS + +        +V+ +I  A ++WG
Sbjct: 1   MEDLDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDV---QNVISEIGDACEKWG 60

Query: 64  FFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWKEV 123
           FF V+NHGVP + ++R+E   + FF   +EEK KV+R++    GY D E TKNV+DWKEV
Sbjct: 61  FFQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEV 119

[Close]

BLAST of HG10005852 vs. TAIR 10
Match: AT3G19010.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 2.8e-23
Identity = 53/122 (43.44%), Postives = 77/122 (63.11%), Query Frame = 0


Query: 4   LEEFDQTFIQRPEHR--LKLAISEADGIPTIDLSPIFNSPGCNFPNDVVRQIASASKEWG 63
           +E+ D T+IQ PEHR       ++ + IP IDLS + +        +V+ +I  A ++WG
Sbjct: 1   MEDLDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDV---QNVISEIGDACEKWG 60

Query: 64  FFLVVNHGVPPEKQRRIEVAAREFFGQSLEEKRKVRRNEGVVTGYFDTELTKNVRDWKEV 123
           FF V+NHGVP + ++R+E   + FF   +EEK KV+R++    GY D E TKNV+DWKEV
Sbjct: 61  FFQVLNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEV 119

The following BLAST results are available for this feature:

BLAST of HG10005852 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887395.1	1.0e-59	80.69	protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida]	[more]
KAG7011675.1	1.7e-54	74.48	putative 2-oxoglutarate-dependent dioxygenase, partial [Cucurbita argyrosperma s...	[more]
KAA0031270.1	8.4e-54	76.39	protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo var. makuwa]	[more]
XP_008454901.1	8.4e-54	76.39	PREDICTED: protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo] >TYK06722.1 protein...	[more]
KAA0031269.1	1.1e-53	75.00	protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo var. makuwa] >TYK06721.1 protei...	[more]

BLAST of HG10005852 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A023GS29	2.3e-09	37.50	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase Diox2 OS=Ruta gra...	[more]
O80449	3.9e-09	36.46	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
Q9LHN8	6.6e-09	27.74	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S...	[more]
O04395	6.6e-09	41.03	Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana OX=3724...	[more]
A0A023GS28	8.6e-09	36.54	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase Diox1 OS=Ruta gra...	[more]

BLAST of HG10005852 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SKK1	4.1e-54	76.39	Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A5D3C5Z7	4.1e-54	76.39	Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BZ66	4.1e-54	76.39	protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 GN=LOC103495206 PE=3 ...	[more]
A0A5A7SPM0	5.3e-54	75.00	Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BZR3	5.3e-54	75.00	LOW QUALITY PROTEIN: protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 ...	[more]

BLAST of HG10005852 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19000.1	7.2e-27	47.24	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.2	7.2e-27	47.24	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.1	2.8e-23	43.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.2	2.8e-23	43.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.3	2.8e-23	43.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 29..132 e-value: 1.6E-26 score: 93.4
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..141 e-value: 1.2E-26 score: 95.7
None	No IPR available	PANTHER	PTHR10209:SF664	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE, NON-HEME DIOXYGENASE DOMAIN-CONTAINING PROTEIN-RELATED	coord: 5..127
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 5..127
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 6..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10005852.1	HG10005852.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding