HG10005848 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10005848
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationChr07: 7020782 .. 7023084 (+)
RNA-Seq ExpressionHG10005848
SyntenyHG10005848
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGAGACCTTGGCCATGGATTCTACCTTCATCCAACCCATCGAGCACCGCCCCAAGCTCAAACCCCTCGACCCCATTGATGATGAAGTTCCAACCATAGATCTCTCCATCTCCCTCTCTCGCACCACCCAACAGCTCGTCTCTGAAATAGCCTCTGCTTGCCAGAACTGGGGTTTCTTCCAGGTAATCAACCATGGAGTTCCCCTCGAGCTGCTCGCGCGAATTGAGAAAGCTGCCAAGTTGTTTTTTGATCAGTCAATGGACGAGAAGAGGAAGGTGAAGAGAGATGCAGCTAATGCTATGGGGTTCCATGACGGTGAGAATACGAAAAACGTTAGGGATTGGAAGGAAGTGTTTGATTTCTTGGTTAAGGATGGGACGTTGATTCCGGCTTCGCATGACCCAAATGACAAGGAATTGAGGGTATTGTGTAATCAGTGGCCGCAATATCCCCCTGGGTTTAGGTACTTTTAATTTCTCTCCAAATTGTTCCTTGCCTTGAATTAAATGCATACCCAACTGATTCCATAGTGTAGAGATTTAGTTCTTGTTTTCTTATCTATATTTGTATTACTTGAAAGAGAATTAAGGGAATTATTGTTGGGTTTGGAAAACTGGCACACCAACTGTTTGTATAAATTCCCCTGTGAAACTGTGAGCTTGCATTTCTTCGTGCATTCTGAGAGTGATTTTGAAGCGATTTCGTATTCAAAATCACTCCAAAATATACTTTTAATCACTCAAAATTAATTTAATATTTAATTTTACGTTTTTAAATGTAATTTTTATGTTATCAAAATTGATTTCGAAGGATTAAAAACATGTTTTAGAATGATTTTAAAAATGACAAAAGTGATTTTAACTATTTGAAAATCACTTCTTAACACACCCTTCATCTACTGCTGGAGGAACTTGTTATTGTGTTGGCTTATGGTATCAGAGAAATGGGTTGTTGGTTTTCTTGAGCAGGGAAACATGTGAGGAGTATGCAAGAGAGTTGGAATTGCTAGCTCATAAGCTACTGGGGCTGATTTCTTTGAGTCTAGGCTCACCTAGTGCCGATGGATTGAAGGGCTATTTCAACGACAAGCAAACAAGCTTGGTAAGGTTGAATCGCTATCCTCCATGCCCGTCTCCGGATCTCGTGCTTGGCATTGGCCGTCACAAAGATGCTGGTGCCTTGACAATCCTTGCTCAAGATGGTGTTGGAGGGCTGCAAGTGAAGAGAAAATCAGATGGTGAATGGATTCCTGTCAGACCAATCCCAAATGCATATATTGTTAACATTGGTGATGTTGTTCAAGTATGTTGCATTTTACATATTATACATAATATAGACATGTACGAAATCTTTCTTAGGTCATGGTGATCTGAAAAGGAGGAGATCTTAAGAACCTGGAAATGACCTCTGAAACTTGTGTGTGAGTAACATTGGTTTAGAAAGGACAGCTTAGTCTAGAGATTTGGTTTTGAAAAATACTTAGGTGTATGTGTCAAATTCTTCTTACTTCTTTTTTGAAATATTTTAATTCTTTTTTTGTGTGTAATGAGAGTGGGATGCATAAAGATGGGTGCATCTTAGTATGCATCCAAGTATTACTTTTTGGTTTTCTGAAGAAAGATTGATAGGAGATGTCAAGAAGTTTACCGTATCTTAAGTGCTTGCCACCCTTTTACACTGCAAAAGAAAGAACACAAGATTTTCCAATGAGCACAAAAGGTGGCAAGCAGGGATTTCAAACTCTGCTTCCTTCTCTATCCAATTCTATCATTAGAGTGGTTGTGACACATAAAACTTCCTTAGCCTTTCACAATAGCTTACAACAGAGATCCATGTTTGGGTAACCAAAACTCTTAGGAAGAGCTAATAGCAAAGAGCCATAGAGGGTCCGCGTGAAATGATTTTCTAAGTGCTTAAAAACAGGACATTTTTTTAAGTAGTTAGAAAATCATTCCAAACAAGCTTAGACTCGTGTGGTAGATAGATTTTGGTCGTTGAAGAGATGAAGCGTAATCGAAGTTCATGGTTGTATGTGCAGGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGGGTGGTGGTGAATACTGAGAAAGAAAGGTTTTCGTTTCCATTCTTCTTCTTTCCAGCTCACCATATGATGGTGAAGCCATTGGAGGAGCTTGTGAATGAGCAAAACCCACCAAAATACAGAGAATACAACTTTGGGAAGTTCTTTGTTTCCAGAAACCGCAGTGATTTCCAGAAGCAAAAAGTGGAAAACATCCAAATTGACCACTTTAAGCTGCTTGTGTGA

mRNA sequence

ATGGGAGAGACCTTGGCCATGGATTCTACCTTCATCCAACCCATCGAGCACCGCCCCAAGCTCAAACCCCTCGACCCCATTGATGATGAAGTTCCAACCATAGATCTCTCCATCTCCCTCTCTCGCACCACCCAACAGCTCGTCTCTGAAATAGCCTCTGCTTGCCAGAACTGGGGTTTCTTCCAGGTAATCAACCATGGAGTTCCCCTCGAGCTGCTCGCGCGAATTGAGAAAGCTGCCAAGTTGTTTTTTGATCAGTCAATGGACGAGAAGAGGAAGGTGAAGAGAGATGCAGCTAATGCTATGGGGTTCCATGACGGTGAGAATACGAAAAACGTTAGGGATTGGAAGGAAGTGTTTGATTTCTTGGTTAAGGATGGGACGTTGATTCCGGCTTCGCATGACCCAAATGACAAGGAATTGAGGGTATTGTGTAATCAGTGGCCGCAATATCCCCCTGGGTTTAGGGAAACATGTGAGGAGTATGCAAGAGAGTTGGAATTGCTAGCTCATAAGCTACTGGGGCTGATTTCTTTGAGTCTAGGCTCACCTAGTGCCGATGGATTGAAGGGCTATTTCAACGACAAGCAAACAAGCTTGGTAAGGTTGAATCGCTATCCTCCATGCCCGTCTCCGGATCTCGTGCTTGGCATTGGCCGTCACAAAGATGCTGGTGCCTTGACAATCCTTGCTCAAGATGGTGTTGGAGGGCTGCAAGTGAAGAGAAAATCAGATGGTGAATGGATTCCTGTCAGACCAATCCCAAATGCATATATTGTTAACATTGGTGATGTTGTTCAAGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGGGTGGTGGTGAATACTGAGAAAGAAAGGTTTTCGTTTCCATTCTTCTTCTTTCCAGCTCACCATATGATGGTGAAGCCATTGGAGGAGCTTGTGAATGAGCAAAACCCACCAAAATACAGAGAATACAACTTTGGGAAGTTCTTTGTTTCCAGAAACCGCAGTGATTTCCAGAAGCAAAAAGTGGAAAACATCCAAATTGACCACTTTAAGCTGCTTGTGTGA

Coding sequence (CDS)

ATGGGAGAGACCTTGGCCATGGATTCTACCTTCATCCAACCCATCGAGCACCGCCCCAAGCTCAAACCCCTCGACCCCATTGATGATGAAGTTCCAACCATAGATCTCTCCATCTCCCTCTCTCGCACCACCCAACAGCTCGTCTCTGAAATAGCCTCTGCTTGCCAGAACTGGGGTTTCTTCCAGGTAATCAACCATGGAGTTCCCCTCGAGCTGCTCGCGCGAATTGAGAAAGCTGCCAAGTTGTTTTTTGATCAGTCAATGGACGAGAAGAGGAAGGTGAAGAGAGATGCAGCTAATGCTATGGGGTTCCATGACGGTGAGAATACGAAAAACGTTAGGGATTGGAAGGAAGTGTTTGATTTCTTGGTTAAGGATGGGACGTTGATTCCGGCTTCGCATGACCCAAATGACAAGGAATTGAGGGTATTGTGTAATCAGTGGCCGCAATATCCCCCTGGGTTTAGGGAAACATGTGAGGAGTATGCAAGAGAGTTGGAATTGCTAGCTCATAAGCTACTGGGGCTGATTTCTTTGAGTCTAGGCTCACCTAGTGCCGATGGATTGAAGGGCTATTTCAACGACAAGCAAACAAGCTTGGTAAGGTTGAATCGCTATCCTCCATGCCCGTCTCCGGATCTCGTGCTTGGCATTGGCCGTCACAAAGATGCTGGTGCCTTGACAATCCTTGCTCAAGATGGTGTTGGAGGGCTGCAAGTGAAGAGAAAATCAGATGGTGAATGGATTCCTGTCAGACCAATCCCAAATGCATATATTGTTAACATTGGTGATGTTGTTCAAGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGGGTGGTGGTGAATACTGAGAAAGAAAGGTTTTCGTTTCCATTCTTCTTCTTTCCAGCTCACCATATGATGGTGAAGCCATTGGAGGAGCTTGTGAATGAGCAAAACCCACCAAAATACAGAGAATACAACTTTGGGAAGTTCTTTGTTTCCAGAAACCGCAGTGATTTCCAGAAGCAAAAAGTGGAAAACATCCAAATTGACCACTTTAAGCTGCTTGTGTGA

Protein sequence

MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGFFQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVFDFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLSLGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQVKRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAHHMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV
Homology
BLAST of HG10005848 vs. NCBI nr
Match: XP_038888870.1 (flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida])

HSP 1 Score: 682.9 bits (1761), Expect = 1.4e-192
Identity = 325/351 (92.59%), Postives = 341/351 (97.15%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           MGETLAMDSTFIQPIEHRPKL+PL+PI++EVPTIDLSISLSRTTQQLVSEIASACQNWGF
Sbjct: 1   MGETLAMDSTFIQPIEHRPKLEPLNPINEEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGVPLELLARI+K AKLFFDQ+++EKRKVKRDAANAMGFHDGENTKNVRDWKEVF
Sbjct: 61  FQVINHGVPLELLARIKKTAKLFFDQTVEEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFLVKD T IPASHDPNDK+LRVL NQWPQYPPGFRETCE+YARELELLAHKLLGLISLS
Sbjct: 121 DFLVKDETFIPASHDPNDKKLRVLRNQWPQYPPGFRETCEDYARELELLAHKLLGLISLS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG   ADGLKGYFND+QTSLVRLN YPPCPSPDLVLG+GRHKDAGALTILAQDGVGGLQV
Sbjct: 181 LGLSGADGLKGYFNDEQTSLVRLNHYPPCPSPDLVLGVGRHKDAGALTILAQDGVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           KRKSD EWIPV+P+PNA+IVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH
Sbjct: 241 KRKSDEEWIPVKPVPNAFIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKL 352
           HMMVKPLEELVNEQNPPKYREYNFGKFF SRNRSDFQKQKVENIQIDHF+L
Sbjct: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENIQIDHFRL 351

BLAST of HG10005848 vs. NCBI nr
Match: XP_022931615.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 635.6 bits (1638), Expect = 2.6e-178
Identity = 299/353 (84.70%), Postives = 329/353 (93.20%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           MGE LA D++FIQPI+HRPKL+P+DPI DEVPTIDLSIS SR+ +QLVS+IASAC+NWGF
Sbjct: 1   MGEALAFDASFIQPIQHRPKLEPVDPI-DEVPTIDLSISDSRSVEQLVSDIASACENWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGVP+EL+AR+E+  KLFFDQ ++EKRKVKRDAANAMG+HDGENTKNVRDWKEVF
Sbjct: 61  FQVINHGVPMELVARMEEVTKLFFDQGVEEKRKVKRDAANAMGYHDGENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFLVKD TL+PASH+P+D  LRVL NQWPQYPPGFRETCEEYA+ELELLA+KLLGLI LS
Sbjct: 121 DFLVKDRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG P AD LKGYF D+Q S+VRLNRYPPC SPDLVLG+GRHKDAGALTILAQDGVGGLQV
Sbjct: 181 LGLPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           +RKSDGEWIPV+PI NAYIVNIGD+VQVWSNDKYESVEHRVVVNTEKERFS PFFFFPAH
Sbjct: 241 RRKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV 354
           H MVKPLEE+VNEQNPPKYREYNFGKFF SRNRSDFQKQKVEN+QIDHF+L V
Sbjct: 301 HTMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFRLPV 352

BLAST of HG10005848 vs. NCBI nr
Match: KAG7022434.1 (Protein DMR6-LIKE OXYGENASE 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 634.0 bits (1634), Expect = 7.6e-178
Identity = 299/353 (84.70%), Postives = 328/353 (92.92%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           MGE LA D++FIQPI+HRPKL+P+DPI DEVPTIDLSIS SR+ +QLVS+IASAC+NWGF
Sbjct: 1   MGEALAFDASFIQPIQHRPKLEPVDPI-DEVPTIDLSISDSRSVEQLVSDIASACENWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGVP+EL+AR+E+  KLFFDQ +DEKRKVKRDAANAMG+HDGENTKNVRDWKEVF
Sbjct: 61  FQVINHGVPMELVARMEEVTKLFFDQGVDEKRKVKRDAANAMGYHDGENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFLVKD TL+PASH+P+D  LRVL NQWPQYPPGFRETCEEYA+ELELLA+KLLGLI LS
Sbjct: 121 DFLVKDRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG   AD LKGYF D+Q S+VRLNRYPPC SPDLVLG+GRHKDAGALTILAQDGVGGLQV
Sbjct: 181 LGLSVADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           +RKSDGEWIPV+PI NAYIVNIGD+VQVWSNDKYESVEHRVVVNTEKERFS PFFFFPAH
Sbjct: 241 RRKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV 354
           H MVKPLEE+VNEQNPPKYREYNFGKFF SRNRSDFQKQKVEN+QIDHF+L V
Sbjct: 301 HTMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFRLPV 352

BLAST of HG10005848 vs. NCBI nr
Match: XP_023530110.1 (protein DMR6-LIKE OXYGENASE 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630.9 bits (1626), Expect = 6.4e-177
Identity = 298/353 (84.42%), Postives = 327/353 (92.63%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           MGE LA D++FIQPI+HRPKL+P+DPI DEVPTIDLSIS SR+ +QLVS+IASAC+NWGF
Sbjct: 1   MGEALAFDASFIQPIQHRPKLEPVDPI-DEVPTIDLSISDSRSVEQLVSDIASACENWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGVP+EL+AR+EK AKLFFDQ ++EKRKVKRDAANAMG+HDGENTKNVRDWKEVF
Sbjct: 61  FQVINHGVPMELVARMEKVAKLFFDQGVEEKRKVKRDAANAMGYHDGENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFLVKD TL+PASH+P+D  LRVL NQWPQYPP F ETCEEYA+ELELLA+KLLGLI  S
Sbjct: 121 DFLVKDRTLVPASHEPDDTNLRVLTNQWPQYPPEFSETCEEYAKELELLAYKLLGLICSS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG P AD LKGYF D+Q S+VRLNRYPPC SPDLVLG+GRHKDAGALTILAQDGVGGLQV
Sbjct: 181 LGLPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           +RKSDGEWIPV+PI NAYIVNIGD+VQVWSNDKYESVEHRVVVNTEKERFS PFFFFPAH
Sbjct: 241 RRKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV 354
           H MVKPLEE+VNEQNPPKYREYNFGKFF SRNRSDFQKQKVEN+QIDHF+L V
Sbjct: 301 HTMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFRLPV 352

BLAST of HG10005848 vs. NCBI nr
Match: XP_022988832.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 630.6 bits (1625), Expect = 8.4e-177
Identity = 299/353 (84.70%), Postives = 327/353 (92.63%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           MGE LA D++FIQPIEHRPKL+ +DPI DEVPTIDLSIS SR+ +QLVS+IASAC+NWGF
Sbjct: 1   MGEALAFDASFIQPIEHRPKLEHVDPI-DEVPTIDLSISDSRSVEQLVSDIASACENWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGVP+ELLAR+EK AKLFFDQ ++EKRKVKRDAANAMG+HDGENTKNVRDWKEVF
Sbjct: 61  FQVINHGVPMELLARMEKVAKLFFDQGIEEKRKVKRDAANAMGYHDGENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFLV+D TL+PASH+P+   LRVL NQWPQYPP FRETCEEYA+ELELLA+KLLGLI LS
Sbjct: 121 DFLVRDRTLVPASHEPDGTSLRVLTNQWPQYPPEFRETCEEYAKELELLAYKLLGLICLS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG P AD LKGYF D+Q S+VRLNRYPPC SPDLVLG+GRHKDAGALTILAQDGVGGLQV
Sbjct: 181 LGLPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           +RKSDGEWIPV+PI NAYIVNIGD+VQVWSNDKYESVEHRVVVNTEKERFS PFFFFPAH
Sbjct: 241 RRKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV 354
           H MVKPLEE+VNEQNPPKYREYNFGKFF SRNRSDFQKQKVEN+QIDHF+L V
Sbjct: 301 HTMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFRLPV 352

BLAST of HG10005848 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 8.4e-55
Identity = 120/328 (36.59%), Postives = 178/328 (54.27%), Query Frame = 0

Query: 9   STFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGFFQVINHGV 68
           S +++P+  RPK+  +    D +P IDL          ++++ A AC + GFFQ+ NHGV
Sbjct: 20  SNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNHGV 79

Query: 69  PLELLARIEKAAKLFFDQSMDEKRK-----VKRDAANAMGFH-DGENTKNVRDWKEVFDF 128
           P E + ++  AA+ FF QS  E+ K      K+    +  F+   E   N RD+  +  +
Sbjct: 80  PEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCY 139

Query: 129 LVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLSLG 188
            ++D                   N+WP  P  FRE   EYA  +  L   LL  IS SLG
Sbjct: 140 PIED-----------------FINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLG 199

Query: 189 SPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQVKR 248
             + D +      K    + +N YP CP P+L  G+  HKDA  +T+L QD V GLQV +
Sbjct: 200 L-AKDRVSNTIG-KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFK 259

Query: 249 KSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAHHM 308
             DG+WI V P+PN +IVN+GD +QV SN+KY+SV HR VVN++ ER S P F+ P+   
Sbjct: 260 --DGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDA 319

Query: 309 MVKPLEELVNEQ--NPPKYREYNFGKFF 329
           ++ P +EL+NE+  +P  YR + + ++F
Sbjct: 320 VISPAQELINEEEDSPAIYRNFTYAEYF 326

BLAST of HG10005848 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.4e-54
Identity = 128/329 (38.91%), Postives = 177/329 (53.80%), Query Frame = 0

Query: 9   STFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGFFQVINHGV 68
           S +++PI  RP L  ++   D +P IDL          +V ++ASAC  +GFFQ+ NHGV
Sbjct: 22  SNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNHGV 81

Query: 69  PLELLARIEKAAKLFFDQSMDEKRKVKRDAAN-------AMGFHDG-ENTKNVRDWKEVF 128
           P   + +++  A+ FF Q   E  +VK  +A+       +  F+ G +   N RD+  + 
Sbjct: 82  PDTTVNKMQTVAREFFHQ--PESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLH 141

Query: 129 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 188
            F ++D                    +WP  P  FRE   EYA  +  L  +LL  IS S
Sbjct: 142 CFPIED-----------------FIEEWPSSPISFREVTAEYATSVRALVLRLLEAISES 201

Query: 189 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 248
           LG  S D +      K    +  N YPPCP P+L  G+  HKD   +T+L QD V GLQV
Sbjct: 202 LGLES-DHISNILG-KHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQV 261

Query: 249 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 308
            +  D +W+ V PIPN +IVNIGD +QV SNDKY+SV HR VVNTE ER S P F+FP+ 
Sbjct: 262 FK--DDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPST 321

Query: 309 HMMVKPLEELVNEQNP-PKYREYNFGKFF 329
             ++ P  ELVNEQ+    YR Y F +++
Sbjct: 322 DAVIGPAHELVNEQDSLAIYRTYPFVEYW 327

BLAST of HG10005848 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 6.7e-52
Identity = 117/328 (35.67%), Postives = 176/328 (53.66%), Query Frame = 0

Query: 6   AMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSR---TTQQLVSEIASACQNWGFFQ 65
           ++   +I+P   RP      P    +P IDL    S    +   +++ I+ AC+ WGFFQ
Sbjct: 37  SLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQ 96

Query: 66  VINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHD--GENTKNVRDWKEVF 125
           V+NHGV  EL+    +  + FF   ++ K           G+    G       DW + +
Sbjct: 97  VVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYY 156

Query: 126 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 185
            FL     L+P  H   D       N+WP +PP  RE  +EY  EL  L+ +++ ++S +
Sbjct: 157 -FL----HLLP--HHLKD------FNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTN 216

Query: 186 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTI-LAQDGVGGLQ 245
           LG       + +  +   + +R+N YP CP P+L LG+  H D G +TI L  D V GLQ
Sbjct: 217 LGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQ 276

Query: 246 VKRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPA 305
           V++  D  WI V+P P+A+IVNIGD +Q+ SN  Y+SVEHRV+VN++KER S  FF+ P 
Sbjct: 277 VRK--DDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPK 336

Query: 306 HHMMVKPLEELVNEQNPPKYREYNFGKF 328
             + ++PL+ELV+  NPP Y    F ++
Sbjct: 337 SDIPIQPLQELVSTHNPPLYPPMTFDQY 349

BLAST of HG10005848 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 6.2e-50
Identity = 115/316 (36.39%), Postives = 165/316 (52.22%), Query Frame = 0

Query: 11  FIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGFFQVINHGVPL 70
           FI+P + +P          E+PTIDL         +LV  IA A + WG FQV NHG+P 
Sbjct: 23  FIRPEKEQPASTTYHGPAPEIPTIDLD---DPVQDRLVRSIAEASREWGIFQVTNHGIPS 82

Query: 71  ELLARIEKAAKLFFDQSMDEKRKVKR--DAANAMGFHDGENTKNVRDWKEVFDFLVKDGT 130
           +L+ +++   K FF+   +EK    R  DA +  G+   +  K V   K   D       
Sbjct: 83  DLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGY-GTKLQKEVEGKKSWVDH------ 142

Query: 131 LIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLSLGSPSADG 190
           L      P+    R     WP+ PP +R   EEYA+ +  +  KL   +SL LG      
Sbjct: 143 LFHRVWPPSSINYRF----WPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLGLGVEGGVL 202

Query: 191 LKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQVKRKSDGEW 250
            +    D    ++++N YPPCP PDL LG+  H D  ALT+L  + V GLQV +  D  W
Sbjct: 203 KEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFK--DDRW 262

Query: 251 IPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAHHMMVKPLE 310
           I  + IPNA +++IGD +++ SN KY++V HR  VN +K R S+P F  P    +V PL 
Sbjct: 263 IDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADTVVGPLP 322

Query: 311 ELVNEQNPPKYREYNF 325
           +LV+++NPPKY+   F
Sbjct: 323 QLVDDENPPKYKAKKF 322

BLAST of HG10005848 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.4e-49
Identity = 117/311 (37.62%), Postives = 168/311 (54.02%), Query Frame = 0

Query: 11  FIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGFFQVINHGVPL 70
           +I+    +P +  +  +  EVP IDLS S  +    LVSE   A + WG FQV+NHG+P 
Sbjct: 23  YIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLVSE---ASKEWGIFQVVNHGIPN 82

Query: 71  ELLARIEKAAKLFFDQSMDEKRKV-KRDAANAMGFHDGENTKNVRDWKEVFDFLVKDGTL 130
           E++ ++++  K FF+   +EK  + K + + ++  +     K V   K   D L     +
Sbjct: 83  EVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQKEVDGKKGWVDHLFH--KI 142

Query: 131 IPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLSLGSPSADGL 190
            P S         +    WP+ PP +RE  EEYA+ L+L+   L   +SL L        
Sbjct: 143 WPPS--------AINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLGLDLEPNSFK 202

Query: 191 KGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQVKRKSDGEWI 250
            G   D    L+++N YPPCP PDL LG+  H D  A+T+L  + V GLQV +  DG W 
Sbjct: 203 DGAGGDDLVYLMKINYYPPCPRPDLALGVA-HTDMSAITVLVPNEVPGLQVYK--DGHWY 262

Query: 251 PVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAHHMMVKPLEE 310
             + IPNA IV+IGD V++ SN KY+SV HR  VN EK R S+P F  P     V P+ +
Sbjct: 263 DCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDHEVGPIPK 317

Query: 311 LVNEQNPPKYR 321
           LVNE+NP K++
Sbjct: 323 LVNEENPAKFK 317

BLAST of HG10005848 vs. ExPASy TrEMBL
Match: A0A6J1EZZ4 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111437780 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.3e-178
Identity = 299/353 (84.70%), Postives = 329/353 (93.20%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           MGE LA D++FIQPI+HRPKL+P+DPI DEVPTIDLSIS SR+ +QLVS+IASAC+NWGF
Sbjct: 1   MGEALAFDASFIQPIQHRPKLEPVDPI-DEVPTIDLSISDSRSVEQLVSDIASACENWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGVP+EL+AR+E+  KLFFDQ ++EKRKVKRDAANAMG+HDGENTKNVRDWKEVF
Sbjct: 61  FQVINHGVPMELVARMEEVTKLFFDQGVEEKRKVKRDAANAMGYHDGENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFLVKD TL+PASH+P+D  LRVL NQWPQYPPGFRETCEEYA+ELELLA+KLLGLI LS
Sbjct: 121 DFLVKDRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG P AD LKGYF D+Q S+VRLNRYPPC SPDLVLG+GRHKDAGALTILAQDGVGGLQV
Sbjct: 181 LGLPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           +RKSDGEWIPV+PI NAYIVNIGD+VQVWSNDKYESVEHRVVVNTEKERFS PFFFFPAH
Sbjct: 241 RRKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV 354
           H MVKPLEE+VNEQNPPKYREYNFGKFF SRNRSDFQKQKVEN+QIDHF+L V
Sbjct: 301 HTMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFRLPV 352

BLAST of HG10005848 vs. ExPASy TrEMBL
Match: A0A6J1JE50 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111486062 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 4.1e-177
Identity = 299/353 (84.70%), Postives = 327/353 (92.63%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           MGE LA D++FIQPIEHRPKL+ +DPI DEVPTIDLSIS SR+ +QLVS+IASAC+NWGF
Sbjct: 1   MGEALAFDASFIQPIEHRPKLEHVDPI-DEVPTIDLSISDSRSVEQLVSDIASACENWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGVP+ELLAR+EK AKLFFDQ ++EKRKVKRDAANAMG+HDGENTKNVRDWKEVF
Sbjct: 61  FQVINHGVPMELLARMEKVAKLFFDQGIEEKRKVKRDAANAMGYHDGENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFLV+D TL+PASH+P+   LRVL NQWPQYPP FRETCEEYA+ELELLA+KLLGLI LS
Sbjct: 121 DFLVRDRTLVPASHEPDGTSLRVLTNQWPQYPPEFRETCEEYAKELELLAYKLLGLICLS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG P AD LKGYF D+Q S+VRLNRYPPC SPDLVLG+GRHKDAGALTILAQDGVGGLQV
Sbjct: 181 LGLPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           +RKSDGEWIPV+PI NAYIVNIGD+VQVWSNDKYESVEHRVVVNTEKERFS PFFFFPAH
Sbjct: 241 RRKSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV 354
           H MVKPLEE+VNEQNPPKYREYNFGKFF SRNRSDFQKQKVEN+QIDHF+L V
Sbjct: 301 HTMVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFRLPV 352

BLAST of HG10005848 vs. ExPASy TrEMBL
Match: A0A5D3C6D5 (Flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00190 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.7e-175
Identity = 300/353 (84.99%), Postives = 325/353 (92.07%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           M +TL +DSTFIQPI HRPKL P   + D+VPTIDLSISLSRTTQ LVSEIASACQNWGF
Sbjct: 1   MEDTLVVDSTFIQPIHHRPKLPPFQ-LTDQVPTIDLSISLSRTTQHLVSEIASACQNWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGV LE LARI+KAAKLFFDQ+++EKRKVKRD+ NA+GF+DGENTKNVRDWKEVF
Sbjct: 61  FQVINHGVSLEKLARIQKAAKLFFDQTVEEKRKVKRDSGNAVGFYDGENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFLVKDGT  PASHDPND +LRVL NQWP+YPPGFRETCEEYARELELLAHKLL LI LS
Sbjct: 121 DFLVKDGTFFPASHDPNDTKLRVLFNQWPEYPPGFRETCEEYARELELLAHKLLRLILLS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG   AD LKGYFND+QTSLVRLNRYPPCPSPDLVLG+GRHKD+GALTILAQD VGGLQV
Sbjct: 181 LGLSDADALKGYFNDEQTSLVRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           +RKSDGEWIPV+PIPNAYI+NIGDVVQVWSNDKYESVEHRVVVNTEKERFSF FFFFPAH
Sbjct: 241 RRKSDGEWIPVKPIPNAYIINIGDVVQVWSNDKYESVEHRVVVNTEKERFSFAFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV 354
           HMMVKPLEELV+EQNPPKYREYNFGK+F SR+R + QKQ+VENIQIDHF+L V
Sbjct: 301 HMMVKPLEELVDEQNPPKYREYNFGKYFASRSRGN-QKQEVENIQIDHFRLPV 351

BLAST of HG10005848 vs. ExPASy TrEMBL
Match: A0A1S3C0D6 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495204 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.7e-175
Identity = 300/353 (84.99%), Postives = 325/353 (92.07%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           M +TL +DSTFIQPI HRPKL P   + D+VPTIDLSISLSRTTQ LVSEIASACQNWGF
Sbjct: 1   MEDTLVVDSTFIQPIHHRPKLPPFQ-LTDQVPTIDLSISLSRTTQHLVSEIASACQNWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGV LE LARI+KAAKLFFDQ+++EKRKVKRD+ NA+GF+DGENTKNVRDWKEVF
Sbjct: 61  FQVINHGVSLEKLARIQKAAKLFFDQTVEEKRKVKRDSGNAVGFYDGENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFLVKDGT  PASHDPND +LRVL NQWP+YPPGFRETCEEYARELELLAHKLL LI LS
Sbjct: 121 DFLVKDGTFFPASHDPNDTKLRVLFNQWPEYPPGFRETCEEYARELELLAHKLLRLILLS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG   AD LKGYFND+QTSLVRLNRYPPCPSPDLVLG+GRHKD+GALTILAQD VGGLQV
Sbjct: 181 LGLSDADALKGYFNDEQTSLVRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           +RKSDGEWIPV+PIPNAYI+NIGDVVQVWSNDKYESVEHRVVVNTEKERFSF FFFFPAH
Sbjct: 241 RRKSDGEWIPVKPIPNAYIINIGDVVQVWSNDKYESVEHRVVVNTEKERFSFAFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV 354
           HMMVKPLEELV+EQNPPKYREYNFGK+F SR+R + QKQ+VENIQIDHF+L V
Sbjct: 301 HMMVKPLEELVDEQNPPKYREYNFGKYFASRSRGN-QKQEVENIQIDHFRLPV 351

BLAST of HG10005848 vs. ExPASy TrEMBL
Match: A0A6J1C2Z2 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111007793 PE=3 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 2.9e-175
Identity = 294/353 (83.29%), Postives = 325/353 (92.07%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPIDDEVPTIDLSISLSRTTQQLVSEIASACQNWGF 60
           MGE LA D  FIQ IEHRP+L+P+D + DEVPTIDLS+S SR+ +QLVSEIASAC+ WGF
Sbjct: 1   MGEALAFDPAFIQAIEHRPELEPIDTV-DEVPTIDLSVSDSRSIEQLVSEIASACEKWGF 60

Query: 61  FQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVF 120
           FQVINHGVP+EL AR++KAAK FFDQ+++EKR VKRDAANAMGFHD ENTKNVRDWKEVF
Sbjct: 61  FQVINHGVPVELHARLDKAAKSFFDQTVEEKRNVKRDAANAMGFHDAENTKNVRDWKEVF 120

Query: 121 DFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLS 180
           DFL++D TLIPASH+P+D +LRVL NQWPQYPPGFRETCEEYARELELLA KLLGLI LS
Sbjct: 121 DFLIRDRTLIPASHEPDDTKLRVLSNQWPQYPPGFRETCEEYARELELLADKLLGLICLS 180

Query: 181 LGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQV 240
           LG P A+GL+GYF D QTS+VRLN YPPCPSP+LVLG+G HKDAGALTILAQDGVGGLQV
Sbjct: 181 LGLPGAEGLRGYFKDDQTSVVRLNHYPPCPSPNLVLGVGHHKDAGALTILAQDGVGGLQV 240

Query: 241 KRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAH 300
           KRKSDGEWIPV+PIPNAYIVNIGD+VQVWSNDKYESVEHR VVNTEKERFSFPFFFFPAH
Sbjct: 241 KRKSDGEWIPVKPIPNAYIVNIGDIVQVWSNDKYESVEHRAVVNTEKERFSFPFFFFPAH 300

Query: 301 HMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKLLV 354
           HMMVKPLEELVN+QNP KYREYN+GKFF +RNRSD+QKQKVENIQIDHF+LLV
Sbjct: 301 HMMVKPLEELVNDQNPSKYREYNWGKFFCTRNRSDYQKQKVENIQIDHFRLLV 352

BLAST of HG10005848 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 461.5 bits (1186), Expect = 6.3e-130
Identity = 223/357 (62.46%), Postives = 274/357 (76.75%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPID----DEVPTIDLSISLSRTTQQ---LVSEIAS 60
           MGE   +D  FIQ  EHRP     +  D    DE+PTIDLS SL  T      +  EIA 
Sbjct: 1   MGE---LDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLS-SLEDTHHDKTAIAKEIAE 60

Query: 61  ACQNWGFFQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNV 120
           AC+ WGFFQVINHG+P  L  R+EK A  FF+ + +EKRKVKRD  N MG+HD E+TKNV
Sbjct: 61  ACKRWGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNV 120

Query: 121 RDWKEVFDFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKL 180
           RDWKE+FDF ++D T++PAS +P D ELR L NQWPQ P  FRE C+EYARE+E LA +L
Sbjct: 121 RDWKEIFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRL 180

Query: 181 LGLISLSLGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQD 240
           L L+S+SLG P  D L G+FN+ QTS +R N YPPCP+P+L LG+GRHKD GALT+LAQD
Sbjct: 181 LELVSISLGLP-GDRLTGFFNE-QTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQD 240

Query: 241 GVGGLQVKRKSDGEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFP 300
            VGGLQV R+SDG+WIPV+PI +A I+N+G+ +QVW+ND+Y S EHRVVVNT KERFS P
Sbjct: 241 SVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIP 300

Query: 301 FFFFPAHHMMVKPLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFK 351
           FFFFP+H   ++PLEEL++E+NPP Y++YN+GKFFVSRNRSDF+K +VENIQIDHFK
Sbjct: 301 FFFFPSHEANIEPLEELISEENPPCYKKYNWGKFFVSRNRSDFKKLEVENIQIDHFK 351

BLAST of HG10005848 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 429.1 bits (1102), Expect = 3.4e-120
Identity = 200/346 (57.80%), Postives = 255/346 (73.70%), Query Frame = 0

Query: 7   MDSTFIQPIEHRPKLKPLDPIDDEVPTIDLS-ISLSRTTQQLVSEIASACQNWGFFQVIN 66
           +D T+IQ  EHR     +    +E+P IDLS +      Q ++SEI  AC+ WGFFQV+N
Sbjct: 4   LDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLN 63

Query: 67  HGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVFDFLVK 126
           HGVP +   R+EK  K+FFD  M+EK KVKRD  N +G+HDGE+TKNV+DWKEVFD   K
Sbjct: 64  HGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFK 123

Query: 127 DGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLSLGSPS 186
           D  +IP++ DP D+ LR++ N+WPQ P  FRE CE YAR  E LA KLL LISLSLG P 
Sbjct: 124 DPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPK 183

Query: 187 ADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQVKRKSD 246
            +    YF + Q S  R+NRYPPCP PDL LG+G HKDA  +++LAQD VGGLQV R+SD
Sbjct: 184 -ERFHDYFKE-QMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSD 243

Query: 247 GEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAHHMMVK 306
           G W P+RP+PNA ++NIG+ +++W+NDKY S EHRVVVNT +ER+S PFF  P+H + VK
Sbjct: 244 GVWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVK 303

Query: 307 PLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKL 352
           PLEELV+ +NPPKY+ Y +GKF+VSRNRSDF+K ++ENIQID FK+
Sbjct: 304 PLEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDFKV 347

BLAST of HG10005848 vs. TAIR 10
Match: AT3G19010.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 393.3 bits (1009), Expect = 2.1e-109
Identity = 184/346 (53.18%), Postives = 237/346 (68.50%), Query Frame = 0

Query: 7   MDSTFIQPIEHRPKLKPLDPIDDEVPTIDLS-ISLSRTTQQLVSEIASACQNWGFFQVIN 66
           +D T+IQ  EHR     +    +E+P IDLS +      Q ++SEI  AC+ WGFFQV+N
Sbjct: 4   LDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLN 63

Query: 67  HGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVFDFLVK 126
           HGVP +   R+EK  K+FFD  M+EK KVKRD  N +G+HDGE+TKNV+DWKEVFD   K
Sbjct: 64  HGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFK 123

Query: 127 DGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLSLGSPS 186
           D  +IP++ DP D+ LR++ N+WPQ P  FRE CE YAR  E LA               
Sbjct: 124 DPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLA--------------- 183

Query: 187 ADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQVKRKSD 246
                            +NRYPPCP PDL LG+G HKDA  +++LAQD VGGLQV R+SD
Sbjct: 184 -----------------INRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSD 243

Query: 247 GEWIPVRPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAHHMMVK 306
           G W P+RP+PNA ++NIG+ +++W+NDKY S EHRVVVNT +ER+S PFF  P+H + VK
Sbjct: 244 GVWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVK 303

Query: 307 PLEELVNEQNPPKYREYNFGKFFVSRNRSDFQKQKVENIQIDHFKL 352
           PLEELV+ +NPPKY+ Y +GKF+VSRNRSDF+K ++ENIQID FK+
Sbjct: 304 PLEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDFKV 317

BLAST of HG10005848 vs. TAIR 10
Match: AT3G19000.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 329.3 bits (843), Expect = 3.7e-90
Identity = 163/275 (59.27%), Postives = 201/275 (73.09%), Query Frame = 0

Query: 1   MGETLAMDSTFIQPIEHRPKLKPLDPID----DEVPTIDLSISLSRTTQQ---LVSEIAS 60
           MGE   +D  FIQ  EHRP     +  D    DE+PTIDLS SL  T      +  EIA 
Sbjct: 1   MGE---LDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLS-SLEDTHHDKTAIAKEIAE 60

Query: 61  ACQNWGFFQVINHGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNV 120
           AC+ WGFFQVINHG+P  L  R+EK A  FF+ + +EKRKVKRD  N MG+HD E+TKNV
Sbjct: 61  ACKRWGFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNV 120

Query: 121 RDWKEVFDFLVKDGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKL 180
           RDWKE+FDF ++D T++PAS +P D ELR L NQWPQ P  FRE C+EYARE+E LA +L
Sbjct: 121 RDWKEIFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRL 180

Query: 181 LGLISLSLGSPSADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQD 240
           L L+S+SLG P  D L G+FN+ QTS +R N YPPCP+P+L LG+GRHKD GALT+LAQD
Sbjct: 181 LELVSISLGLP-GDRLTGFFNE-QTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQD 240

Query: 241 GVGGLQVKRKSDGEWIPVRPIPNAYIVNIGDVVQV 269
            VGGLQV R+SDG+WIPV+PI +A I+N+G+ +QV
Sbjct: 241 SVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQV 269

BLAST of HG10005848 vs. TAIR 10
Match: AT3G19010.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.2e-83
Identity = 145/263 (55.13%), Postives = 185/263 (70.34%), Query Frame = 0

Query: 7   MDSTFIQPIEHRPKLKPLDPIDDEVPTIDLS-ISLSRTTQQLVSEIASACQNWGFFQVIN 66
           +D T+IQ  EHR     +    +E+P IDLS +      Q ++SEI  AC+ WGFFQV+N
Sbjct: 4   LDPTYIQAPEHRSDSNFIPNQPEEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLN 63

Query: 67  HGVPLELLARIEKAAKLFFDQSMDEKRKVKRDAANAMGFHDGENTKNVRDWKEVFDFLVK 126
           HGVP +   R+EK  K+FFD  M+EK KVKRD  N +G+HDGE+TKNV+DWKEVFD   K
Sbjct: 64  HGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFK 123

Query: 127 DGTLIPASHDPNDKELRVLCNQWPQYPPGFRETCEEYARELELLAHKLLGLISLSLGSPS 186
           D  +IP++ DP D+ LR++ N+WPQ P  FRE CE YAR  E LA KLL LISLSLG P 
Sbjct: 124 DPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPK 183

Query: 187 ADGLKGYFNDKQTSLVRLNRYPPCPSPDLVLGIGRHKDAGALTILAQDGVGGLQVKRKSD 246
            +    YF + Q S  R+NRYPPCP PDL LG+G HKDA  +++LAQD VGGLQV R+SD
Sbjct: 184 -ERFHDYFKE-QMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSD 243

Query: 247 GEWIPVRPIPNAYIVNIGDVVQV 269
           G W P+RP+PNA ++NIG+ ++V
Sbjct: 244 GVWFPIRPVPNALVINIGNCMEV 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888870.11.4e-19292.59flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida][more]
XP_022931615.12.6e-17884.70flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata][more]
KAG7022434.17.6e-17884.70Protein DMR6-LIKE OXYGENASE 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023530110.16.4e-17784.42protein DMR6-LIKE OXYGENASE 1-like [Cucurbita pepo subsp. pepo][more]
XP_022988832.18.4e-17784.70flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9ZSA78.4e-5536.59Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1[more]
Q9ZSA81.4e-5438.91Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1[more]
Q9FFF66.7e-5235.67Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1[more]
Q9ZWQ96.2e-5036.39Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ... [more]
Q9M5471.4e-4937.62Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian... [more]
Match NameE-valueIdentityDescription
A0A6J1EZZ41.3e-17884.70flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JE504.1e-17784.70flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5D3C6D51.7e-17584.99Flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3C0D61.7e-17584.99flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1C2Z22.9e-17583.29flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
AT3G19000.16.3e-13062.462-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G19010.13.4e-12057.802-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G19010.32.1e-10953.182-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G19000.23.7e-9059.272-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G19010.21.2e-8355.132-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 286..304
score: 35.41
coord: 51..68
score: 50.51
coord: 221..242
score: 40.91
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 6..343
NoneNo IPR availablePANTHERPTHR10209:SF727DIRIGENT PROTEINcoord: 6..343
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 9..336
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 31..150
e-value: 1.2E-27
score: 97.0
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..338
e-value: 1.5E-106
score: 358.5
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 199..298
e-value: 1.9E-29
score: 102.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 197..298
score: 13.936672

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10005848.1HG10005848.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity