HG10005841 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10005841
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	diphthamide biosynthesis protein 3-like
Location	Chr07: 6917970 .. 6918227 (+)
RNA-Seq Expression	HG10005841
Synteny	HG10005841

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCATACGACGACGTGGAGATTGAAGACATGGAGTGGAACGAGGAACTGCAGGCCTTCACGTACCCTTGCCCCTGCGGCGACTTATTCCAGATCACCAAGGAGGATCTTAAGCTCGGCGAAGAGATTGCTCGGTGCCCTAGCTGCTCTCTTTACATCACAGTCATCTACAACATGGAAGATTTTCTCGGAGACTCTAATCAGAAGAATAACAAAGGACTTGAGCCCTCTAAACAGCAGCCTATCGTCGTCGCTTAA

mRNA sequence

ATGTCATACGACGACGTGGAGATTGAAGACATGGAGTGGAACGAGGAACTGCAGGCCTTCACGTACCCTTGCCCCTGCGGCGACTTATTCCAGATCACCAAGGAGGATCTTAAGCTCGGCGAAGAGATTGCTCGGTGCCCTAGCTGCTCTCTTTACATCACAGTCATCTACAACATGGAAGATTTTCTCGGAGACTCTAATCAGAAGAATAACAAAGGACTTGAGCCCTCTAAACAGCAGCCTATCGTCGTCGCTTAA

Coding sequence (CDS)

ATGTCATACGACGACGTGGAGATTGAAGACATGGAGTGGAACGAGGAACTGCAGGCCTTCACGTACCCTTGCCCCTGCGGCGACTTATTCCAGATCACCAAGGAGGATCTTAAGCTCGGCGAAGAGATTGCTCGGTGCCCTAGCTGCTCTCTTTACATCACAGTCATCTACAACATGGAAGATTTTCTCGGAGACTCTAATCAGAAGAATAACAAAGGACTTGAGCCCTCTAAACAGCAGCCTATCGTCGTCGCTTAA

Protein sequence

MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSNQKNNKGLEPSKQQPIVVA

Homology

[Close]

BLAST of HG10005841 vs. NCBI nr
Match: XP_038887330.1 (diphthamide biosynthesis protein 3-like [Benincasa hispida] >XP_038887331.1 diphthamide biosynthesis protein 3-like [Benincasa hispida])

HSP 1 Score: 184.9 bits (468), Expect = 2.9e-43
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFLGDSNQKNNKGLEPSKQQPIVVA
Sbjct: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 85

[Close]

BLAST of HG10005841 vs. NCBI nr
Match: XP_022972367.1 (diphthamide biosynthesis protein 3-like [Cucurbita maxima])

HSP 1 Score: 179.9 bits (455), Expect = 9.4e-42
Identity = 82/85 (96.47%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFLGDS+ KNNKGL+PSKQQPIVVA
Sbjct: 61 DFLGDSDHKNNKGLQPSKQQPIVVA 85

[Close]

BLAST of HG10005841 vs. NCBI nr
Match: XP_008454889.1 (PREDICTED: diphthamide biosynthesis protein 3-like [Cucumis melo] >XP_008454890.1 PREDICTED: diphthamide biosynthesis protein 3-like [Cucumis melo] >KAA0031262.1 diphthamide biosynthesis protein 3-like [Cucumis melo var. makuwa] >TYK06714.1 diphthamide biosynthesis protein 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 179.1 bits (453), Expect = 1.6e-41
Identity = 82/85 (96.47%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDM+WNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMDWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFL DSNQKNNKGLEPSKQ PIVVA
Sbjct: 61 DFLDDSNQKNNKGLEPSKQHPIVVA 85

[Close]

BLAST of HG10005841 vs. NCBI nr
Match: XP_022136247.1 (diphthamide biosynthesis protein 3-like [Momordica charantia])

HSP 1 Score: 177.6 bits (449), Expect = 4.7e-41
Identity = 79/85 (92.94%), Postives = 85/85 (100.00%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDMEWN+ELQA+TYPCPCGDLFQITKEDL+LGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMEWNQELQAYTYPCPCGDLFQITKEDLRLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFLGDS+QKNNKG++PSKQQPIVVA
Sbjct: 61 DFLGDSDQKNNKGIQPSKQQPIVVA 85

[Close]

BLAST of HG10005841 vs. NCBI nr
Match: XP_004137002.1 (diphthamide biosynthesis protein 3 [Cucumis sativus] >KGN43974.1 hypothetical protein Csa_017712 [Cucumis sativus])

HSP 1 Score: 176.4 bits (446), Expect = 1.0e-40
Identity = 81/85 (95.29%), Postives = 82/85 (96.47%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDMEWN+ELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMEWNDELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFL  SNQKNNKGLEPSKQ PIVVA
Sbjct: 61 DFLDHSNQKNNKGLEPSKQHPIVVA 85

[Close]

BLAST of HG10005841 vs. ExPASy Swiss-Prot
Match: Q21102 (DPH3 homolog OS=Caenorhabditis elegans OX=6239 GN=K01H12.1 PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.7e-17
Identity = 36/61 (59.02%), Postives = 50/61 (81.97%), Query Frame = 0


Query: 3  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDF 62
          +D+VEIED E++EE   + YPCPCGD F+I +E L++GE++A+CPSCSL I VIY+ EDF
Sbjct: 5  HDEVEIEDFEFDEEKDVYHYPCPCGDRFEIPREMLEMGEDVAQCPSCSLLIRVIYDPEDF 64

Query: 63 L 64
          +
Sbjct: 65 V 65

[Close]

BLAST of HG10005841 vs. ExPASy Swiss-Prot
Match: Q9VGQ9 (DPH3 homolog OS=Drosophila melanogaster OX=7227 GN=CG14701 PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.7e-17
Identity = 38/68 (55.88%), Postives = 53/68 (77.94%), Query Frame = 0


Query: 3  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDF 62
          +D+VEIED E++EE + + YPCPCGD FQI+KE+L  GEE+A CPSCSL I VIY+ E F
Sbjct: 5  HDEVEIEDFEYDEEEEMYYYPCPCGDRFQISKEELIEGEEVATCPSCSLVIKVIYDPEMF 64

Query: 63 LGDSNQKN 71
            + ++++
Sbjct: 65 KAEEDEES 72

[Close]

BLAST of HG10005841 vs. ExPASy Swiss-Prot
Match: Q6VUC1 (DPH3 homolog OS=Cricetulus griseus OX=10029 GN=DPH3 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.9e-17
Identity = 35/61 (57.38%), Postives = 49/61 (80.33%), Query Frame = 0


Query: 3  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDF 62
          +D+VEIED +++E+ + + YPCPCGD F ITKEDL+ GE++A CPSCSL I VIY+ + F
Sbjct: 5  HDEVEIEDFQYDEDSETYFYPCPCGDNFSITKEDLENGEDVATCPSCSLIIKVIYDKDQF 64

Query: 63 L 64
          +
Sbjct: 65 M 65

[Close]

BLAST of HG10005841 vs. ExPASy Swiss-Prot
Match: Q96FX2 (DPH3 homolog OS=Homo sapiens OX=9606 GN=DPH3 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.7e-17
Identity = 35/61 (57.38%), Postives = 49/61 (80.33%), Query Frame = 0


Query: 3  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDF 62
          +D+VEIED +++E+ + + YPCPCGD F ITKEDL+ GE++A CPSCSL I VIY+ + F
Sbjct: 5  HDEVEIEDFQYDEDSETYFYPCPCGDNFSITKEDLENGEDVATCPSCSLIIKVIYDKDQF 64

Query: 63 L 64
          +
Sbjct: 65 V 65

[Close]

BLAST of HG10005841 vs. ExPASy Swiss-Prot
Match: Q4R312 (DPH3 homolog OS=Macaca fascicularis OX=9541 GN=DPH3 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.7e-17
Identity = 35/61 (57.38%), Postives = 49/61 (80.33%), Query Frame = 0


Query: 3  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDF 62
          +D+VEIED +++E+ + + YPCPCGD F ITKEDL+ GE++A CPSCSL I VIY+ + F
Sbjct: 5  HDEVEIEDFQYDEDSETYFYPCPCGDNFSITKEDLENGEDVATCPSCSLIIKVIYDKDQF 64

Query: 63 L 64
          +
Sbjct: 65 V 65

[Close]

BLAST of HG10005841 vs. ExPASy TrEMBL
Match: A0A6J1I4L9 (diphthamide biosynthesis protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470942 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.6e-42
Identity = 82/85 (96.47%), Postives = 84/85 (98.82%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFLGDS+ KNNKGL+PSKQQPIVVA
Sbjct: 61 DFLGDSDHKNNKGLQPSKQQPIVVA 85

[Close]

BLAST of HG10005841 vs. ExPASy TrEMBL
Match: A0A1S3BZL5 (diphthamide biosynthesis protein 3-like OS=Cucumis melo OX=3656 GN=LOC103495198 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 7.8e-42
Identity = 82/85 (96.47%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDM+WNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMDWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFL DSNQKNNKGLEPSKQ PIVVA
Sbjct: 61 DFLDDSNQKNNKGLEPSKQHPIVVA 85

[Close]

BLAST of HG10005841 vs. ExPASy TrEMBL
Match: A0A5A7SJN1 (Diphthamide biosynthesis protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00150 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 7.8e-42
Identity = 82/85 (96.47%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDM+WNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMDWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFL DSNQKNNKGLEPSKQ PIVVA
Sbjct: 61 DFLDDSNQKNNKGLEPSKQHPIVVA 85

[Close]

BLAST of HG10005841 vs. ExPASy TrEMBL
Match: A0A6J1C519 (diphthamide biosynthesis protein 3-like OS=Momordica charantia OX=3673 GN=LOC111007992 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.3e-41
Identity = 79/85 (92.94%), Postives = 85/85 (100.00%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDMEWN+ELQA+TYPCPCGDLFQITKEDL+LGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMEWNQELQAYTYPCPCGDLFQITKEDLRLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFLGDS+QKNNKG++PSKQQPIVVA
Sbjct: 61 DFLGDSDQKNNKGIQPSKQQPIVVA 85

[Close]

BLAST of HG10005841 vs. ExPASy TrEMBL
Match: A0A0A0K4W9 (DPH-type MB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G075060 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.0e-41
Identity = 81/85 (95.29%), Postives = 82/85 (96.47%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDMEWN+ELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMEWNDELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DFL  SNQKNNKGLEPSKQ PIVVA
Sbjct: 61 DFLDHSNQKNNKGLEPSKQHPIVVA 85

[Close]

BLAST of HG10005841 vs. TAIR 10
Match: AT2G15910.2 (CSL zinc finger domain-containing protein )

HSP 1 Score: 145.2 bits (365), Expect = 2.4e-35
Identity = 67/85 (78.82%), Postives = 74/85 (87.06%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDMEWNEE+QA+TYPCPCGDLFQITKEDL+LGEEIA CPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMEWNEEIQAYTYPCPCGDLFQITKEDLRLGEEIANCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPIVVA 86
          DF  D+ +KNN   EP  + P+ VA
Sbjct: 61 DFQNDT-KKNN---EPKTRHPVAVA 81

[Close]

BLAST of HG10005841 vs. TAIR 10
Match: AT2G15910.1 (CSL zinc finger domain-containing protein )

HSP 1 Score: 141.4 bits (355), Expect = 3.5e-34
Identity = 65/82 (79.27%), Postives = 72/82 (87.80%), Query Frame = 0


Query: 1  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNME 60
          MSYDDVEIEDMEWNEE+QA+TYPCPCGDLFQITKEDL+LGEEIA CPSCSLYITVIYNME
Sbjct: 1  MSYDDVEIEDMEWNEEIQAYTYPCPCGDLFQITKEDLRLGEEIANCPSCSLYITVIYNME 60

Query: 61 DFLGDSNQKNNKGLEPSKQQPI 83
          DF  D+ +KNN   EP  + P+
Sbjct: 61 DFQNDT-KKNN---EPKTRHPV 78

The following BLAST results are available for this feature:

BLAST of HG10005841 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887330.1	2.9e-43	100.00	diphthamide biosynthesis protein 3-like [Benincasa hispida] >XP_038887331.1 diph...	[more]
XP_022972367.1	9.4e-42	96.47	diphthamide biosynthesis protein 3-like [Cucurbita maxima]	[more]
XP_008454889.1	1.6e-41	96.47	PREDICTED: diphthamide biosynthesis protein 3-like [Cucumis melo] >XP_008454890....	[more]
XP_022136247.1	4.7e-41	92.94	diphthamide biosynthesis protein 3-like [Momordica charantia]	[more]
XP_004137002.1	1.0e-40	95.29	diphthamide biosynthesis protein 3 [Cucumis sativus] >KGN43974.1 hypothetical pr...	[more]

BLAST of HG10005841 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q21102	1.7e-17	59.02	DPH3 homolog OS=Caenorhabditis elegans OX=6239 GN=K01H12.1 PE=3 SV=1	[more]
Q9VGQ9	1.7e-17	55.88	DPH3 homolog OS=Drosophila melanogaster OX=7227 GN=CG14701 PE=3 SV=1	[more]
Q6VUC1	2.9e-17	57.38	DPH3 homolog OS=Cricetulus griseus OX=10029 GN=DPH3 PE=3 SV=1	[more]
Q96FX2	3.7e-17	57.38	DPH3 homolog OS=Homo sapiens OX=9606 GN=DPH3 PE=1 SV=1	[more]
Q4R312	3.7e-17	57.38	DPH3 homolog OS=Macaca fascicularis OX=9541 GN=DPH3 PE=3 SV=1	[more]

BLAST of HG10005841 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I4L9	4.6e-42	96.47	diphthamide biosynthesis protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470...	[more]
A0A1S3BZL5	7.8e-42	96.47	diphthamide biosynthesis protein 3-like OS=Cucumis melo OX=3656 GN=LOC103495198 ...	[more]
A0A5A7SJN1	7.8e-42	96.47	Diphthamide biosynthesis protein 3-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1C519	2.3e-41	92.94	diphthamide biosynthesis protein 3-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A0A0K4W9	5.0e-41	95.29	DPH-type MB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G075060...	[more]

BLAST of HG10005841 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15910.2	2.4e-35	78.82	CSL zinc finger domain-containing protein	[more]
AT2G15910.1	3.5e-34	79.27	CSL zinc finger domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007872	DPH-type metal-binding domain	PFAM	PF05207	zf-CSL	coord: 4..58 e-value: 4.6E-22 score: 77.5
IPR007872	DPH-type metal-binding domain	PROSITE	PS51074	DPH_MB	coord: 2..58 score: 23.350922
IPR036671	DPH-type metal-binding domain superfamily	GENE3D	3.10.660.10	DPH Zinc finger	coord: 1..82 e-value: 1.2E-29 score: 103.9
IPR036671	DPH-type metal-binding domain superfamily	SUPERFAMILY	144217	CSL zinc finger	coord: 2..72
None	No IPR available	PANTHER	PTHR21454:SF2	DPH3 HOMOLOG	coord: 2..73
IPR044248	Diphthamide biosynthesis protein 3/4-like	PANTHER	PTHR21454	DPH3 HOMOLOG-RELATED	coord: 2..73

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10005841.1	HG10005841.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046872	metal ion binding