HG10005824 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10005824
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRibonuclease H protein
LocationChr07: 6513915 .. 6514421 (-)
RNA-Seq ExpressionHG10005824
SyntenyHG10005824
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCACCGGGAGGAGGGGAGATGAAGTTGAATGTCGATACTCTATGCGTGTCTAATGTGGGAGTGGGGTTGGGGGCTATTATTCCTGACTCTCAAGGAAATGTGGTGAAAACTGCGAATAAATTTGAGGAGGGAGTGGTGAATGTTTTAGCTGCTGAAGCGTTAGCACTCTTGTTTAGAATTCAAATGGCCTTTGATTTGCGTCTAACTAATTTATGGGTAGAATTTGATTCTCATAAATTAGTTAGAGTCATTCAAAATTATTCTATGCAAAATTATTCAATTGGTGGAATTCTAGAAGAGATTAGAGACTGGATGGGTAAAATTAATGTTAGGGGTCTCTTTTTTATTTTGTGTGCTTGTAATGGGGTCGCTCACTCTTTAGCTAGATGGGCGTCTACTAATAGAGTCGATGGTCAACGGTCTATTGTGGTTCATAGATGGCTTGGTCATTTTGTTGAGCTTGATGCTTATTTTTGTAATCCACTAGACGGTATTTATGTTTGA

mRNA sequence

ATGTCACCGGGAGGAGGGGAGATGAAGTTGAATGTCGATACTCTATGCGTGTCTAATGTGGGAGTGGGGTTGGGGGCTATTATTCCTGACTCTCAAGGAAATGTGGTGAAAACTGCGAATAAATTTGAGGAGGGAGTGGTGAATGTTTTAGCTGCTGAAGCGTTAGCACTCTTGTTTAGAATTCAAATGGCCTTTGATTTGCGTCTAACTAATTTATGGGTAGAATTTGATTCTCATAAATTAGTTAGAGTCATTCAAAATTATTCTATGCAAAATTATTCAATTGGTGGAATTCTAGAAGAGATTAGAGACTGGATGGGTAAAATTAATGTTAGGGGTCTCTTTTTTATTTTGTGTGCTTGTAATGGGGTCGCTCACTCTTTAGCTAGATGGGCGTCTACTAATAGAGTCGATGGTCAACGGTCTATTGTGGTTCATAGATGGCTTGGTCATTTTGTTGAGCTTGATGCTTATTTTTGTAATCCACTAGACGGTATTTATGTTTGA

Coding sequence (CDS)

ATGTCACCGGGAGGAGGGGAGATGAAGTTGAATGTCGATACTCTATGCGTGTCTAATGTGGGAGTGGGGTTGGGGGCTATTATTCCTGACTCTCAAGGAAATGTGGTGAAAACTGCGAATAAATTTGAGGAGGGAGTGGTGAATGTTTTAGCTGCTGAAGCGTTAGCACTCTTGTTTAGAATTCAAATGGCCTTTGATTTGCGTCTAACTAATTTATGGGTAGAATTTGATTCTCATAAATTAGTTAGAGTCATTCAAAATTATTCTATGCAAAATTATTCAATTGGTGGAATTCTAGAAGAGATTAGAGACTGGATGGGTAAAATTAATGTTAGGGGTCTCTTTTTTATTTTGTGTGCTTGTAATGGGGTCGCTCACTCTTTAGCTAGATGGGCGTCTACTAATAGAGTCGATGGTCAACGGTCTATTGTGGTTCATAGATGGCTTGGTCATTTTGTTGAGCTTGATGCTTATTTTTGTAATCCACTAGACGGTATTTATGTTTGA

Protein sequence

MSPGGGEMKLNVDTLCVSNVGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRIQMAFDLRLTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCACNGVAHSLARWASTNRVDGQRSIVVHRWLGHFVELDAYFCNPLDGIYV
Homology
BLAST of HG10005824 vs. NCBI nr
Match: XP_042974660.1 (uncharacterized protein LOC122306292 [Carya illinoinensis])

HSP 1 Score: 75.1 bits (183), Expect = 6.5e-10
Identity = 43/131 (32.82%), Postives = 71/131 (54.20%), Query Frame = 0

Query: 2   SPGGGEMKLNVDTLCVSNVGV-GLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFR 61
           SP  G  KLNVD    SN  V G+G I+ +S+G V+ +A K E  V+  +  EALA++  
Sbjct: 44  SPPSGVFKLNVDGAFSSNGSVSGIGVIVRNSKGEVIMSAAKKELSVMTAVEVEALAIIRG 103

Query: 62  IQMAFDLRLTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCA 121
           +Q+  DL + NL +E DS  +V+          + G ++ E ++ + +     +  +  +
Sbjct: 104 LQLCIDLEIQNLVIESDSLLVVKEFNKQGSSKATFGNVIREAKELVTRFGTCEVQHVNRS 163

Query: 122 CNGVAHSLARW 132
           CN  AHSLA++
Sbjct: 164 CNEAAHSLAKF 174

BLAST of HG10005824 vs. NCBI nr
Match: XP_042988698.1 (uncharacterized protein LOC122316231 [Carya illinoinensis])

HSP 1 Score: 73.9 bits (180), Expect = 1.4e-09
Identity = 46/131 (35.11%), Postives = 70/131 (53.44%), Query Frame = 0

Query: 2   SPGGGEMKLNVDTLCVSNVGV-GLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFR 61
           SP  G  KLNVD    SN  V G GAI+ DS+G V+ +A K E  ++ V+  EALA+L  
Sbjct: 558 SPPSGVFKLNVDGAFSSNGSVAGTGAIVRDSKGEVIMSATKKEVSIMIVVEVEALAILRG 617

Query: 62  IQMAFDLRLTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCA 121
           +Q    L + NL +E DS  +VR          + G ++ E ++ M +  +  +  +  +
Sbjct: 618 LQFCIHLGIHNLIIESDSLLVVREFNMQGSSKATFGNVIREAKELMTRFGICEVQHVSRS 677

Query: 122 CNGVAHSLARW 132
            N  AHSLA++
Sbjct: 678 YNKAAHSLAKF 688

BLAST of HG10005824 vs. NCBI nr
Match: XP_042979948.1 (uncharacterized protein LOC122310131 [Carya illinoinensis])

HSP 1 Score: 73.2 bits (178), Expect = 2.5e-09
Identity = 44/131 (33.59%), Postives = 70/131 (53.44%), Query Frame = 0

Query: 3   PGGGEMKLNVDTLCVSN-VGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRI 62
           P  G  KLNVD     N    G+GAI+ DS+G V+  A+K E  V +  + E LA+L  +
Sbjct: 11  PQPGTFKLNVDGAIFQNQQSAGIGAILRDSKGEVLMAASKRESAVGDATSIEMLAILRGL 70

Query: 63  QMAFDLRLTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCAC 122
           Q+   L + +L +E D+  LV+ +Q        +G ++ + ++ M    V  + + + +C
Sbjct: 71  QLTLHLGIQHLIIESDALTLVKEMQKTEPSMTLVGNVIRDTKELMSCFRVCDVRYTMRSC 130

Query: 123 NGVAHSLARWA 133
           N VAH LAR A
Sbjct: 131 NEVAHKLARHA 141

BLAST of HG10005824 vs. NCBI nr
Match: KAF5445494.1 (hypothetical protein F2P56_034541 [Juglans regia])

HSP 1 Score: 72.0 bits (175), Expect = 5.5e-09
Identity = 47/131 (35.88%), Postives = 72/131 (54.96%), Query Frame = 0

Query: 3   PGGGEMKLNVDTLCVSNVG-VGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRI 62
           P  G +KLNVD +  ++VG  G+G I+ + +G VV  A K E+        E +A L  +
Sbjct: 119 PPPGFLKLNVDGVMFADVGKAGIGVILRNKEGQVVMAAIKKEDMADEPATIELIAWLRGL 178

Query: 63  QMAFDLRLTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCAC 122
           Q+   L ++ L VEFD   LV+ +Q     + + G +L+++RD M   N   +  +    
Sbjct: 179 QLCLPLGISKLVVEFDCLLLVQELQESHEFSSTNGNLLKDVRDLMKHFNELHIKHVYREG 238

Query: 123 NGVAHSLARWA 133
           NGVAHSLAR+A
Sbjct: 239 NGVAHSLARFA 249

BLAST of HG10005824 vs. NCBI nr
Match: XP_030485460.1 (uncharacterized protein LOC115702143 [Cannabis sativa])

HSP 1 Score: 71.6 bits (174), Expect = 7.1e-09
Identity = 42/124 (33.87%), Postives = 66/124 (53.23%), Query Frame = 0

Query: 10  LNVD-TLCVSNVGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRIQMAFDLR 69
           +N D +L     G GL A+I D +G +V  A  F  G ++VL AEA A+   IQ+A    
Sbjct: 224 INTDASLIAGQQGCGLSAVIRDPKGGLVVAATSFIPGCLSVLLAEANAVHLGIQLAIRWS 283

Query: 70  LTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCACNGVAHSL 129
           +TN  V  DSH L++ + + +      G ++++I+    K       F   +CN VA+SL
Sbjct: 284 ITNAQVGSDSHSLIKALNSDTSYPTDWGQLVQDIKLLRNKFQTLQFLFFTRSCNRVANSL 343

Query: 130 ARWA 133
           A+W+
Sbjct: 344 AKWS 347

BLAST of HG10005824 vs. ExPASy TrEMBL
Match: A0A7J6I5R5 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_013359 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.5e-09
Identity = 40/119 (33.61%), Postives = 64/119 (53.78%), Query Frame = 0

Query: 14  TLCVSNVGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRIQMAFDLRLTNLW 73
           +L     G GL A+I D +G +V  A  F  G ++VL AEA A+   IQ+A    +TN  
Sbjct: 618 SLIAGQKGCGLSAVIRDPKGGLVVAATSFIPGCLSVLLAEANAVHLGIQLAIRWSITNAQ 677

Query: 74  VEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCACNGVAHSLARWA 133
           V  DSH L++ + + +      G ++++I+  + K       F   +CN VA+SLA+W+
Sbjct: 678 VGSDSHSLIKALNSDTSYPTDWGQLVQDIKLLINKFQTLQFLFFTRSCNRVANSLAKWS 736

BLAST of HG10005824 vs. ExPASy TrEMBL
Match: A0A7J6HML3 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_005782 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.5e-09
Identity = 42/124 (33.87%), Postives = 66/124 (53.23%), Query Frame = 0

Query: 10  LNVD-TLCVSNVGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRIQMAFDLR 69
           +N D +L     G GL A+I D +G +V  A  F  G ++VL AEA A+   IQ+A    
Sbjct: 567 INTDASLIAGQQGCGLSAVIRDPKGGLVVAATSFIPGCLSVLLAEANAVHLGIQLAIRWS 626

Query: 70  LTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCACNGVAHSL 129
           +TN  V  DSH L++ + + +      G ++++I+    K       F   +CN VA+SL
Sbjct: 627 ITNAQVGSDSHSLIKALNSDTSYPTDWGQLVQDIKLLRNKFQTLQFLFFTRSCNRVANSL 686

Query: 130 ARWA 133
           A+W+
Sbjct: 687 AKWS 690

BLAST of HG10005824 vs. ExPASy TrEMBL
Match: A0A803QSC8 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.5e-09
Identity = 42/124 (33.87%), Postives = 66/124 (53.23%), Query Frame = 0

Query: 10  LNVD-TLCVSNVGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRIQMAFDLR 69
           +N D +L     G GL A+I D +G +V  A  F  G ++VL AEA A+   IQ+A    
Sbjct: 224 INTDASLIAGQQGCGLSAVIRDPKGGLVVAATSFIPGCLSVLLAEANAVHLGIQLAIRWS 283

Query: 70  LTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCACNGVAHSL 129
           +TN  V  DSH L++ + + +      G ++++I+    K       F   +CN VA+SL
Sbjct: 284 ITNAQVGSDSHSLIKALNSDTSYPTDWGQLVQDIKLLRNKFQTLQFLFFTRSCNRVANSL 343

Query: 130 ARWA 133
           A+W+
Sbjct: 344 AKWS 347

BLAST of HG10005824 vs. ExPASy TrEMBL
Match: A0A7J6HCI0 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_012655 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 4.5e-09
Identity = 41/124 (33.06%), Postives = 66/124 (53.23%), Query Frame = 0

Query: 10  LNVD-TLCVSNVGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRIQMAFDLR 69
           +N D +L     G GL A+I D +G +V  A  F  G +++L AEA A+   IQ+A    
Sbjct: 340 INTDASLIAGQQGCGLSAVIRDPKGGLVVAATSFIPGCLSILLAEANAVHLGIQLAIRWS 399

Query: 70  LTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCACNGVAHSL 129
           +TN  V  DSH L++ + + +      G ++++I+    K       F   +CN VA+SL
Sbjct: 400 ITNAQVGSDSHSLIKALNSDTSYPTDWGHLVQDIKLLRNKFQTLQFLFFTRSCNRVANSL 459

Query: 130 ARWA 133
           A+W+
Sbjct: 460 AKWS 463

BLAST of HG10005824 vs. ExPASy TrEMBL
Match: A0A7J6GCK1 (Uncharacterized protein (Fragment) OS=Cannabis sativa OX=3483 GN=G4B88_012792 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 4.5e-09
Identity = 41/124 (33.06%), Postives = 66/124 (53.23%), Query Frame = 0

Query: 10  LNVD-TLCVSNVGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRIQMAFDLR 69
           +N D +L     G GL A+I D +G +V  A  F  G +++L AEA A+   IQ+A    
Sbjct: 197 INTDASLIAGQQGCGLSAVIRDPKGGLVVAATSFIPGCLSILLAEANAVHLGIQLAIRWS 256

Query: 70  LTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCACNGVAHSL 129
           +TN  V  DSH L++ + + +      G ++++I+    K       F   +CN VA+SL
Sbjct: 257 ITNAQVGSDSHSLIKALNSDTSYPTDWGHLVQDIKLLRNKFQTLQFLFFTRSCNRVANSL 316

Query: 130 ARWA 133
           A+W+
Sbjct: 317 AKWS 320

BLAST of HG10005824 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 5.1e-05
Identity = 33/105 (31.43%), Postives = 50/105 (47.62%), Query Frame = 0

Query: 25  GAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRIQMAFDLRLTNLWVEFDSHKLVRV 84
           G  I +  G++V   N   +     L AEAL  L  +Q+ +   L  +W E DS  LV +
Sbjct: 232 GWTIRECNGHIVLCGNAKLQSSTCSLHAEALGFLHALQVIWAHGLRYVWFESDSKSLVTL 291

Query: 85  IQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCACNGVAHSLA 130
           I N    +  +G ++ +IR WM K+    L F+    N  A +LA
Sbjct: 292 INN-GEDHSLLGTLIYDIRHWMLKLPYCSLEFVNRERNSAADALA 335

BLAST of HG10005824 vs. TAIR 10
Match: AT3G23320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 41.6 bits (96), Expect = 7.4e-04
Identity = 48/163 (29.45%), Postives = 67/163 (41.10%), Query Frame = 0

Query: 3   PGGGEMKLNVDTLCVSN----VGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALL 62
           PG   +K N D   VSN       GL  II +SQG  +       +G   +  AE  AL+
Sbjct: 33  PGAEWVKCNYD---VSNHAGRQDSGLRWIIRNSQGTCLDCGCGKFQGRQTIKEAECTALI 92

Query: 63  FRIQMAFDLRLTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFIL 122
           + IQ A+DL    +  E D+  + R+I+N    N  +   LE I+ W          F  
Sbjct: 93  WAIQCAWDLGYRRVEFEGDNITVNRLIRN-KETNPRLRYYLEFIQQWSKAFTTVKFTFRH 152

Query: 123 CACNGVAHSLARWASTNRVDGQRSIVVHRWLGHFVELDAYFCN 162
              N     LA+ A  N ++        R+L  FV  DA   N
Sbjct: 153 REQNVCVDVLAKKAVANHINHTLYHFCPRFLMSFVNKDAEHVN 191

BLAST of HG10005824 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 41.2 bits (95), Expect = 9.6e-04
Identity = 36/131 (27.48%), Postives = 58/131 (44.27%), Query Frame = 0

Query: 3   PGGGEMKLNVD-TLCVSNVGVGLGAIIPDSQGNVVKTANKFEEGVVNVLAAEALALLFRI 62
           PG  ++K N D +    N   GLG I+ +SQG V++      +G +    AE   L++ I
Sbjct: 108 PGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTLIWAI 167

Query: 63  QMAFDLRLTNLWVEFDSHKLVRVIQNYSMQNYSIGGILEEIRDWMGKINVRGLFFILCAC 122
           Q ++      +  E D+  + R+I N    N  +   L+ I+ W+         F     
Sbjct: 168 QASYGFGHKKVIFEGDNQTITRMI-NTKSSNPRLQHFLDTIQSWIPSFESIEFSFKHREQ 227

Query: 123 NGVAHSLARWA 133
           NG A  LA+ A
Sbjct: 228 NGCADFLAKQA 237

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_042974660.16.5e-1032.82uncharacterized protein LOC122306292 [Carya illinoinensis][more]
XP_042988698.11.4e-0935.11uncharacterized protein LOC122316231 [Carya illinoinensis][more]
XP_042979948.12.5e-0933.59uncharacterized protein LOC122310131 [Carya illinoinensis][more]
KAF5445494.15.5e-0935.88hypothetical protein F2P56_034541 [Juglans regia][more]
XP_030485460.17.1e-0933.87uncharacterized protein LOC115702143 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A7J6I5R53.5e-0933.61Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_013359 PE=4 SV=1[more]
A0A7J6HML33.5e-0933.87Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_005782 PE=4 SV=1[more]
A0A803QSC83.5e-0933.87Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A7J6HCI04.5e-0933.06Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_012655 PE=4 SV=1[more]
A0A7J6GCK14.5e-0933.06Uncharacterized protein (Fragment) OS=Cannabis sativa OX=3483 GN=G4B88_012792 PE... [more]
Match NameE-valueIdentityDescription
AT2G02650.15.1e-0531.43Ribonuclease H-like superfamily protein [more]
AT3G23320.17.4e-0429.45Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G65005.19.6e-0427.48Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 5..134
e-value: 1.6E-8
score: 36.6
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 11..132
e-value: 3.4E-18
score: 65.6
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 2..143
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 2..143

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10005824.1HG10005824.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity