HG10005519 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10005519
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	subtilisin-like protease SBT5.3
Location	Chr07: 3274945 .. 3275326 (+)
RNA-Seq Expression	HG10005519
Synteny	HG10005519

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGCTCATTCCCACGACGGTCGAAAACCGGCAAATATCGTCACCAATTCTCACCATGAATTCCTCCGACCGTTTCTCGAAAGGTACGTCTCTAAAACATTGACTTTATCATTCTACTGTAACCTACCAGCTTAAGCTGTATTTTTTAGATTTCGTCGTACAGTTTAATACGATACGAAAATGAGAGTAGTAAGTGTTATGTTTGATAGGTTTAATTTAACGGTTTTGATTGCTTCATGATTGTTTTTTCTCTTTTAGTGGAGAGGAGTTTACAGATGATGTCATCTTTTATTCGTATACAAGACACATCAATGGCTTTGCAGCAATGTTGGAAGATGAAGTTGCTGCTCAATTAGCTAGTAATCTCTCTCTCTCTTGA

mRNA sequence

ATGGGAGCTCATTCCCACGACGGTCGAAAACCGGCAAATATCGTCACCAATTCTCACCATGAATTCCTCCGACCGTTTCTCGAAAGTGGAGAGGAGTTTACAGATGATGTCATCTTTTATTCGTATACAAGACACATCAATGGCTTTGCAGCAATGTTGGAAGATGAAGTTGCTGCTCAATTAGCTAGTAATCTCTCTCTCTCTTGA

Coding sequence (CDS)

ATGGGAGCTCATTCCCACGACGGTCGAAAACCGGCAAATATCGTCACCAATTCTCACCATGAATTCCTCCGACCGTTTCTCGAAAGTGGAGAGGAGTTTACAGATGATGTCATCTTTTATTCGTATACAAGACACATCAATGGCTTTGCAGCAATGTTGGAAGATGAAGTTGCTGCTCAATTAGCTAGTAATCTCTCTCTCTCTTGA

Protein sequence

MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQLASNLSLS

Homology

[Close]

BLAST of HG10005519 vs. NCBI nr
Match: XP_011658647.1 (subtilisin-like protease SBT5.3 [Cucumis sativus] >KAE8645706.1 hypothetical protein Csa_020514 [Cucumis sativus])

HSP 1 Score: 115.5 bits (288), Expect = 1.7e-22
Identity = 53/64 (82.81%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1   MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
           MGAHSH G+KPA++V NSHHEFL+PFL+SGEEFT DVIFYSYTRHINGFAAMLEDEVA Q
Sbjct: 37  MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQ 96

Query: 61  LASN 65
           LA +
Sbjct: 97  LAKH 100

[Close]

BLAST of HG10005519 vs. NCBI nr
Match: KAA0059266.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 115.5 bits (288), Expect = 1.7e-22
Identity = 53/64 (82.81%), Postives = 60/64 (93.75%), Query Frame = 0


Query: 1   MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
           MGAHSH GRKPA++V +SHH+FL+PFL+SGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38  MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97

Query: 61  LASN 65
           LA +
Sbjct: 98  LAKH 101

[Close]

BLAST of HG10005519 vs. NCBI nr
Match: TYK03381.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 115.5 bits (288), Expect = 1.7e-22
Identity = 53/64 (82.81%), Postives = 60/64 (93.75%), Query Frame = 0


Query: 1   MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
           MGAHSH GRKPA++V +SHH+FL+PFL+SGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38  MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97

Query: 61  LASN 65
           LA +
Sbjct: 98  LAKH 101

[Close]

BLAST of HG10005519 vs. NCBI nr
Match: XP_008462019.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo])

HSP 1 Score: 115.5 bits (288), Expect = 1.7e-22
Identity = 53/64 (82.81%), Postives = 60/64 (93.75%), Query Frame = 0


Query: 1   MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
           MGAHSH GRKPA++V +SHH+FL+PFL+SGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38  MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97

Query: 61  LASN 65
           LA +
Sbjct: 98  LAKH 101

[Close]

BLAST of HG10005519 vs. NCBI nr
Match: XP_023554401.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 112.1 bits (279), Expect = 1.9e-21
Identity = 52/64 (81.25%), Postives = 57/64 (89.06%), Query Frame = 0


Query: 1  MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
          MGAHSH GRKPANIV NSH + LRP+LESG EF +DVIFYSYTRHINGFAAMLEDEVAA+
Sbjct: 35 MGAHSHGGRKPANIVANSHRDLLRPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAAR 94

Query: 61 LASN 65
          LA +
Sbjct: 95 LAQH 98

[Close]

BLAST of HG10005519 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 58.9 bits (141), Expect = 2.5e-08
Identity = 33/68 (48.53%), Postives = 43/68 (63.24%), Query Frame = 0


Query: 1   MGAHSHDGRKPAN----IVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDE 60
           MGAHSH G  P +    + T+SH++ L     S E+   + I YSY RHINGFAA+LE+E
Sbjct: 36  MGAHSH-GPSPTSADLELATDSHYDLLGSIFGSREK-AKEAIIYSYNRHINGFAALLEEE 95

Query: 61  VAAQLASN 65
            AA +A N
Sbjct: 96  EAADIAKN 101

[Close]

BLAST of HG10005519 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.7e-07
Identity = 30/66 (45.45%), Postives = 42/66 (63.64%), Query Frame = 0


Query: 2   GAHSHDG---RKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVA 61
           GAHSH G       + V  +H++FL  F  S E  T D IFYSYT+HINGFAA L+ ++A
Sbjct: 37  GAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERAT-DAIFYSYTKHINGFAAHLDHDLA 96

Query: 62  AQLASN 65
            +++ +
Sbjct: 97  YEISKH 101

[Close]

BLAST of HG10005519 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 5.3e-06
Identity = 29/67 (43.28%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 1   MGAHSHDGR-KPANI--VTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEV 60
           +G+H+H  +   A++  V +SH  FL  F+ S E    + IFYSY RHINGFAA+L++  
Sbjct: 46  LGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN-AKEAIFYSYKRHINGFAAILDENE 105

Query: 61  AAQLASN 65
           AA++A +
Sbjct: 106 AAEIAKH 111

[Close]

BLAST of HG10005519 vs. ExPASy TrEMBL
Match: A0A5A7UYI5 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G002010 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.4e-23
Identity = 53/64 (82.81%), Postives = 60/64 (93.75%), Query Frame = 0


Query: 1   MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
           MGAHSH GRKPA++V +SHH+FL+PFL+SGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38  MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97

Query: 61  LASN 65
           LA +
Sbjct: 98  LAKH 101

[Close]

BLAST of HG10005519 vs. ExPASy TrEMBL
Match: A0A5D3BUG6 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84663G00160 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.4e-23
Identity = 53/64 (82.81%), Postives = 60/64 (93.75%), Query Frame = 0


Query: 1   MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
           MGAHSH GRKPA++V +SHH+FL+PFL+SGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38  MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97

Query: 61  LASN 65
           LA +
Sbjct: 98  LAKH 101

[Close]

BLAST of HG10005519 vs. ExPASy TrEMBL
Match: A0A0A0K313 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G030520 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.4e-23
Identity = 53/64 (82.81%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1  MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
          MGAHSH G+KPA++V NSHHEFL+PFL+SGEEFT DVIFYSYTRHINGFAAMLEDEVA Q
Sbjct: 1  MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQ 60

Query: 61 LASN 65
          LA +
Sbjct: 61 LAKH 64

[Close]

BLAST of HG10005519 vs. ExPASy TrEMBL
Match: A0A1S3CG03 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500491 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.4e-23
Identity = 53/64 (82.81%), Postives = 60/64 (93.75%), Query Frame = 0


Query: 1   MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
           MGAHSH GRKPA++V +SHH+FL+PFL+SGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38  MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97

Query: 61  LASN 65
           LA +
Sbjct: 98  LAKH 101

[Close]

BLAST of HG10005519 vs. ExPASy TrEMBL
Match: A0A6J1HWD2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468122 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 6.0e-21
Identity = 51/64 (79.69%), Postives = 57/64 (89.06%), Query Frame = 0


Query: 1   MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
           MGAHSH GRKPA+IV +SH E LRP+LESG EF +DVIFYSYTRHINGFAAMLEDEVAA+
Sbjct: 57  MGAHSHGGRKPADIVADSHRELLRPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAAR 116

Query: 61  LASN 65
           LA +
Sbjct: 117 LAKH 120

[Close]

BLAST of HG10005519 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.6e-08
Identity = 30/66 (45.45%), Postives = 42/66 (63.64%), Query Frame = 0


Query: 2   GAHSHDG---RKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVA 61
           GAHSH G       + V  +H++FL  F  S E  T D IFYSYT+HINGFAA L+ ++A
Sbjct: 37  GAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERAT-DAIFYSYTKHINGFAAHLDHDLA 96

Query: 62  AQLASN 65
            +++ +
Sbjct: 97  YEISKH 101

[Close]

BLAST of HG10005519 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 51.2 bits (121), Expect = 3.8e-07
Identity = 29/67 (43.28%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 1   MGAHSHDGR-KPANI--VTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEV 60
           +G+H+H  +   A++  V +SH  FL  F+ S E    + IFYSY RHINGFAA+L++  
Sbjct: 46  LGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN-AKEAIFYSYKRHINGFAAILDENE 105

Query: 61  AAQLASN 65
           AA++A +
Sbjct: 106 AAEIAKH 111

[Close]

BLAST of HG10005519 vs. TAIR 10
Match: AT1G66210.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 42.0 bits (97), Expect = 2.3e-04
Identity = 24/64 (37.50%), Postives = 35/64 (54.69%), Query Frame = 0


Query: 1   MGAHSHDGRKPANIVTNSHHEFLRPFLESGEEFTDDVIFYSYTRHINGFAAMLEDEVAAQ 60
           +G   HD     NIVT SHH+ L P L S ++ + + + YSY    +GFAA L    A +
Sbjct: 45  LGERQHD---DPNIVTESHHDILGPLLGS-KKASHESMIYSYRHGFSGFAAKLTSSQARE 104

Query: 61  LASN 65
           L+ +
Sbjct: 105 LSGH 104

The following BLAST results are available for this feature:

BLAST of HG10005519 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011658647.1	1.7e-22	82.81	subtilisin-like protease SBT5.3 [Cucumis sativus] >KAE8645706.1 hypothetical pro...	[more]
KAA0059266.1	1.7e-22	82.81	subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa]	[more]
TYK03381.1	1.7e-22	82.81	subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008462019.1	1.7e-22	82.81	PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo]	[more]
XP_023554401.1	1.9e-21	81.25	subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo]	[more]

BLAST of HG10005519 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I1N462	2.5e-08	48.53	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
Q9ZSP5	3.7e-07	45.45	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
F4JXC5	5.3e-06	43.28	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]

BLAST of HG10005519 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UYI5	8.4e-23	82.81	Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A5D3BUG6	8.4e-23	82.81	Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A0A0K313	8.4e-23	82.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G030520 PE=3 SV=1	[more]
A0A1S3CG03	8.4e-23	82.81	subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1035004...	[more]
A0A6J1HWD2	6.0e-21	79.69	subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of HG10005519 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04160.1	2.6e-08	45.45	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59810.1	3.8e-07	43.28	Subtilase family protein	[more]
AT1G66210.1	2.3e-04	37.50	Subtilisin-like serine endopeptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 1..67 e-value: 2.4E-12 score: 49.1
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 10..63 e-value: 1.2E-6 score: 29.1
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 1..62
None	No IPR available	PANTHER	PTHR43806:SF49	SUBTILISIN-LIKE PROTEASE	coord: 1..62

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10005519.1	HG10005519.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity