HG10005427 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10005427
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionglucosidase 2 subunit beta
LocationChr07: 2473248 .. 2475455 (-)
RNA-Seq ExpressionHG10005427
SyntenyHG10005427
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCGCCTCGTTCTCCAAACTCCACCAGCTTCGTTTCGCTGCTTTCCATCTGTTTCTTCTTCGCTTCTGTTCGCTCTGTTCCATCACTCATCGGAGTTCATCCCCTCGGTACTCTTCTCTCTCTCTCTCTCTGACTCTGTGCTTATACTCCCCCTGGATCTGATCTTACGAAGCTCAAAAATGGAATTTGTAGCCCCATTTCGCCCTTCCTCTAGCTAATTCCCCCTTTTGGGGTTTACCCATTTTGTGAATTGATGTTAAATTCGGTTAACTTTCCCGAATTGTAATGTGTGTTTGATTGCAGATGAGAAGTATTATTCCTCGGAGGTCATAAAATGCAAGGATGGTTCTAGATCTTTCGCTATTGATCGTCTCAACGATGATTTCTGTGACTGCGTTGATGGAACCGATGAGCCTGGTGCATTCTTAGTTCTTACTGAATTTTAATCATTAGAGTTACTGCCGTTATAAGCTGAACCTCAAGTTGAAATGGCTGTGAAATTGTGTAATGAACTAAGGCAACAATTACTAGCCATTGTAATCTGTCTTAAATAGGGACATGGAAAAGTTTACCATGTTTATTCTAGAAAAGCATGACTTGGTTTTTCTTTCTGAATTTTCACCAACATGTTCAGAAATGGAATGTTGATAAGTATCTACTTTTTGGCCAATTGTTTTAATGGCTTGCATCTCCGTCAATACCGAGACCAAAACCAAAACAAAATTTTAACTTCTTTTAAGTGACACATCCAAAGGAACAATTTTTTTGGGCTTTCTCAATGAAATCTCATTCCCTCCTCGTCATAGACTCTTTCCAGTAAAATTACATACATAATATTTGGGAAAGTTTTTGTAAGATTTGGGGAATACATACATAATAATGGTCAGTCCCAATCTTCGTCCTAGTGGGAATTCCACCTAAAAAAAATCGATGGTTTGGATGTGCCATTAAGCTAAATTTTGTTTTGGTCTCAATATTGATCCTGGCCAAGATGCACCGGGATGAATCATTAAAATAATTGACCAAAAAAGGTGCTATTTGTGAAATGTGGAAAACTTTGAGTGCCGTACAATTACCTATAGGATAGGTTCTGCATCAGAATGATACTGGACTAATAAGCAATAAATCCAAATTCCAGTATGGGCAATTGGTCATGTAAACAAAAGGAAAACAAACCTTCCCAAATAGCCCAAGAACAGCCACCCACACACAACACAGTGCACGGATCAGGCAGGTTTTTTTTCTGTGCTCTGCTGCTGCTGGTTCATGGATTTGACCTTTGATCTTTGTATTTATATCTGTAGGTACTTCTGCCTGTGCAAGAGGGAAATTCTATTGTCGGAATATGGGAAGCACACCACGATTTATATTTTCTTCCCGTGTCAATGATCGTATTTGTGGTAACTATCTAATAAATCTTTTGTTTTCCTTCTTTTTTTATGCATGTGGCTCAATTGAATTTTGATCAAAAGAAGAAAAGCGTGTCGATTGAAACACCATAACTTTGTTGACGTAGATTTTAGAACAACCTTTCTGTAATGCAATGATTATAGTCTTATGAGTCATGCGCTAGTTGTCCTTGTCTTGATTATTTGTTCTTCCTGCTTTATGTTAAGACAATTTAAGGTCTCTATTCTGGTTAAAATCTGCAAATAGCGGTTGAAACTTGACAATCAACATATTCCATAACTCTCAAAATAATCCTTACAGACCATTTCGTACCATGTACAAAATTTATATGCATTTTTTTTTACTTATGTTGAATCCACTGTTAATTCAGAAACTTGGGGTTTTCATTTCATCTTTGAAATTAAATTCTAGTTCCCTGTTCAATATATTTGTAGATTGCTGTGATGGAAGTGATGAATATGATGGTAATATCTTTTGCCCGAACACATGTGTCATGGGGGGAAATATACTGTACAAGAGCAACAATGATATTTCAACTACTCGAGATGTTGATGTAGTCAACAGAAAAGTTAAGGAAGAAATCACCAAGGAGGATATGTTCCAAAAGCTAATTGGTAGGATTTTTGCTTCAATAATTACAAAACACAGATACACAATACTCAACTAATTGTTGTAACTTGCAATTTCAGGCTTGAAGCTAGTAATAATCCTACAGGTAGCTCTAACAAGTTTTGCAGTCCTCATTTGGGTAAATCGCTGTCGTGTGAAATCTAAGAGAAGACGACATCATTGA

mRNA sequence

ATGGAAGCGCCTCGTTCTCCAAACTCCACCAGCTTCGTTTCGCTGCTTTCCATCTGTTTCTTCTTCGCTTCTGTTCGCTCTGTTCCATCACTCATCGGAGTTCATCCCCTCGATGAGAAGTATTATTCCTCGGAGGTCATAAAATGCAAGGATGGTTCTAGATCTTTCGCTATTGATCGTCTCAACGATGATTTCTGTGACTGCGTTGATGGAACCGATGAGCCTGGTACTTCTGCCTGTGCAAGAGGGAAATTCTATTGTCGGAATATGGGAAGCACACCACGATTTATATTTTCTTCCCGTGTCAATGATCGTATTTGTGATTGCTGTGATGGAAGTGATGAATATGATGGTAATATCTTTTGCCCGAACACATGTGTCATGGGGGGAAATATACTGTACAAGAGCAACAATGATATTTCAACTACTCGAGATGTTGATGTAGTCAACAGAAAAGTTAAGGAAGAAATCACCAAGGAGGATATGTTCCAAAAGCTAATTGGCTTGAAGCTAGTAATAATCCTACAGGTAGCTCTAACAAGTTTTGCAGTCCTCATTTGGGTAAATCGCTGTCGTGTGAAATCTAAGAGAAGACGACATCATTGA

Coding sequence (CDS)

ATGGAAGCGCCTCGTTCTCCAAACTCCACCAGCTTCGTTTCGCTGCTTTCCATCTGTTTCTTCTTCGCTTCTGTTCGCTCTGTTCCATCACTCATCGGAGTTCATCCCCTCGATGAGAAGTATTATTCCTCGGAGGTCATAAAATGCAAGGATGGTTCTAGATCTTTCGCTATTGATCGTCTCAACGATGATTTCTGTGACTGCGTTGATGGAACCGATGAGCCTGGTACTTCTGCCTGTGCAAGAGGGAAATTCTATTGTCGGAATATGGGAAGCACACCACGATTTATATTTTCTTCCCGTGTCAATGATCGTATTTGTGATTGCTGTGATGGAAGTGATGAATATGATGGTAATATCTTTTGCCCGAACACATGTGTCATGGGGGGAAATATACTGTACAAGAGCAACAATGATATTTCAACTACTCGAGATGTTGATGTAGTCAACAGAAAAGTTAAGGAAGAAATCACCAAGGAGGATATGTTCCAAAAGCTAATTGGCTTGAAGCTAGTAATAATCCTACAGGTAGCTCTAACAAGTTTTGCAGTCCTCATTTGGGTAAATCGCTGTCGTGTGAAATCTAAGAGAAGACGACATCATTGA

Protein sequence

MEAPRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNIFCPNTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALTSFAVLIWVNRCRVKSKRRRHH
Homology
BLAST of HG10005427 vs. NCBI nr
Match: XP_038887466.1 (glucosidase 2 subunit beta [Benincasa hispida])

HSP 1 Score: 383.3 bits (983), Expect = 1.3e-102
Identity = 186/201 (92.54%), Postives = 193/201 (96.02%), Query Frame = 0

Query: 1   MEAPRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDR 60
           ME PRSP  TSFVSLLSICFFF SVRSVPSLIGVHPLDEKYY SEVIKCKDGS+SF IDR
Sbjct: 1   METPRSPKPTSFVSLLSICFFFDSVRSVPSLIGVHPLDEKYYYSEVIKCKDGSKSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNILYKSNNDISTTRDVD-VVNRKVKEEITKEDMFQKLIGLKLVIILQVAL 180
           FCPNTCVMGGNI+YKSNNDISTTRDVD +VNRKV+EEIT+ED+FQKLIGLKLVIILQVAL
Sbjct: 121 FCPNTCVMGGNIVYKSNNDISTTRDVDIIVNRKVQEEITEEDIFQKLIGLKLVIILQVAL 180

Query: 181 TSFAVLIWVNRCRVKSKRRRH 201
           TSFA+LIW NRCRVKSKRRRH
Sbjct: 181 TSFAILIWANRCRVKSKRRRH 201

BLAST of HG10005427 vs. NCBI nr
Match: XP_022136263.1 (glucosidase 2 subunit beta [Momordica charantia])

HSP 1 Score: 377.9 bits (969), Expect = 5.6e-101
Identity = 181/200 (90.50%), Postives = 190/200 (95.00%), Query Frame = 0

Query: 1   MEAPRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDR 60
           MEA R P STSFVS++SICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSF IDR
Sbjct: 1   MEAGRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+FIFSS VNDRICDCCDGSDEYDGNI
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALT 180
           FCPNTCVMGGNI YKSNN ISTT+DV +VNRKVKEEITKED+FQKL+GLKLVI+LQVALT
Sbjct: 121 FCPNTCVMGGNIAYKSNNYISTTQDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180

Query: 181 SFAVLIWVNRCRVKSKRRRH 201
           SFAV IWVNRCR +SKRRRH
Sbjct: 181 SFAVFIWVNRCRARSKRRRH 200

BLAST of HG10005427 vs. NCBI nr
Match: XP_004144515.1 (glucosidase 2 subunit beta [Cucumis sativus] >XP_031744257.1 glucosidase 2 subunit beta [Cucumis sativus] >KGN43490.1 hypothetical protein Csa_020313 [Cucumis sativus])

HSP 1 Score: 367.5 bits (942), Expect = 7.5e-98
Identity = 178/197 (90.36%), Postives = 184/197 (93.40%), Query Frame = 0

Query: 4   PRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDRLND 63
           PRSP STSF SLLSIC FF SVRS PS IGVHPLDEKYYSSEVIKCKDGSRSF IDRLND
Sbjct: 3   PRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLND 62

Query: 64  DFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNIFCP 123
           +FCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVND ICDCCDGSDEY+GNIFCP
Sbjct: 63  NFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCP 122

Query: 124 NTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALTSFA 183
           NTCVMGGN +YKS NDISTTRDVD+V RKVKEEITKED+FQKL GLKLVIILQVALTSFA
Sbjct: 123 NTCVMGGN-MYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTSFA 182

Query: 184 VLIWVNRCRVKSKRRRH 201
           +LIW NRCRVKSKRRRH
Sbjct: 183 ILIWANRCRVKSKRRRH 198

BLAST of HG10005427 vs. NCBI nr
Match: XP_008455474.1 (PREDICTED: glucosidase 2 subunit beta isoform X1 [Cucumis melo] >XP_016901681.1 PREDICTED: glucosidase 2 subunit beta isoform X1 [Cucumis melo] >KAA0059098.1 glucosidase 2 subunit beta isoform X1 [Cucumis melo var. makuwa] >TYJ99622.1 glucosidase 2 subunit beta isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 367.1 bits (941), Expect = 9.8e-98
Identity = 179/200 (89.50%), Postives = 187/200 (93.50%), Query Frame = 0

Query: 1   MEAPRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDR 60
           MEA RSP STSFVSLLSIC FF SVR+ PS +GVHPLDEKYYSSEVIKCKDGSRSF IDR
Sbjct: 1   MEA-RSPKSTSFVSLLSICLFFTSVRAAPSFLGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVND ICDCCDGSDEY+GNI
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNI 120

Query: 121 FCPNTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALT 180
           FCPNTCVMGGN +YKSNND+STTRDVD+V RKVKEEITKED+FQKL GLKLVIILQVALT
Sbjct: 121 FCPNTCVMGGN-MYKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALT 180

Query: 181 SFAVLIWVNRCRVKSKRRRH 201
            FA+LIW NRCRVKSKRRRH
Sbjct: 181 IFAILIWANRCRVKSKRRRH 198

BLAST of HG10005427 vs. NCBI nr
Match: XP_022952390.1 (glucosidase 2 subunit beta [Cucurbita moschata])

HSP 1 Score: 366.3 bits (939), Expect = 1.7e-97
Identity = 177/200 (88.50%), Postives = 184/200 (92.00%), Query Frame = 0

Query: 1   MEAPRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDR 60
           MEA  SP S SFV LLS CFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSF IDR
Sbjct: 1   MEAGHSPKSMSFVLLLSFCFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRF+FSSRVND ICDCCDGSDEYDGNI
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFLFSSRVNDHICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALT 180
           FCPNTCVMGGN  YK++  IST+RDVDV+NRKVKEEITKED+F +LIGLKLVIILQVALT
Sbjct: 121 FCPNTCVMGGNAAYKNSYYISTSRDVDVINRKVKEEITKEDLFHRLIGLKLVIILQVALT 180

Query: 181 SFAVLIWVNRCRVKSKRRRH 201
            FAVLIW NRCR KSKRRRH
Sbjct: 181 CFAVLIWANRCRAKSKRRRH 200

BLAST of HG10005427 vs. ExPASy Swiss-Prot
Match: Q9FM96 (Glucosidase 2 subunit beta OS=Arabidopsis thaliana OX=3702 GN=PSL4 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.0e-37
Identity = 82/161 (50.93%), Postives = 101/161 (62.73%), Query Frame = 0

Query: 11  SFVSLLSICFFFASVRSVPSLIGVHPLDEKYY-SSEVIKCKDGSRSFAIDRLNDDFCDCV 70
           SF+ LL+     +S       +G+ P DEKYY SS  IKCKDGS+ F   +LNDDFCDC 
Sbjct: 17  SFLLLLASAIRSSSSPPNDPFLGISPQDEKYYKSSSEIKCKDGSKKFTKAQLNDDFCDCS 76

Query: 71  DGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNIFCPNTCVMG 130
           DGTDEPGTSAC  GKFYCRN G +P  +FSSRVND ICDCCDGSDEYDG++ C NTC   
Sbjct: 77  DGTDEPGTSACPTGKFYCRNAGHSPVILFSSRVNDGICDCCDGSDEYDGHVSCQNTCWEA 136

Query: 131 GNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLK 171
           G    ++      T +  +V R+ + E  K  + +    LK
Sbjct: 137 GKAARENLKKKIETYNQGLVIRRQEIEQAKVGLEKDAAELK 177

BLAST of HG10005427 vs. ExPASy Swiss-Prot
Match: A2WNF5 (Glucosidase 2 subunit beta OS=Oryza sativa subsp. indica OX=39946 GN=OsI_01383 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.5e-37
Identity = 72/116 (62.07%), Postives = 82/116 (70.69%), Query Frame = 0

Query: 15  LLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDRLNDDFCDCVDGTDE 74
           LL I    A+ R     +G+ P DE Y+   VI+C+DGS  FA D+LNDDFCDC DGTDE
Sbjct: 11  LLRISASAAASRPPLDTLGIPPQDEAYFRGGVIRCRDGSGRFARDKLNDDFCDCPDGTDE 70

Query: 75  PGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNIFCPNTCVMGG 131
           PGTSAC  GKFYC+N G +P  IFSSRVND ICDCCDGSDEYD N+ C NTC   G
Sbjct: 71  PGTSACPEGKFYCQNAGHSPITIFSSRVNDGICDCCDGSDEYDSNVTCKNTCWEAG 126

BLAST of HG10005427 vs. ExPASy Swiss-Prot
Match: Q5NBP9 (Glucosidase 2 subunit beta OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0276800 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.5e-37
Identity = 72/116 (62.07%), Postives = 82/116 (70.69%), Query Frame = 0

Query: 15  LLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDRLNDDFCDCVDGTDE 74
           LL I    A+ R     +G+ P DE Y+   VI+C+DGS  FA D+LNDDFCDC DGTDE
Sbjct: 11  LLRISASAAASRPPLDTLGIPPQDEAYFRGGVIRCRDGSGRFARDKLNDDFCDCPDGTDE 70

Query: 75  PGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNIFCPNTCVMGG 131
           PGTSAC  GKFYC+N G +P  IFSSRVND ICDCCDGSDEYD N+ C NTC   G
Sbjct: 71  PGTSACPEGKFYCQNAGHSPITIFSSRVNDGICDCCDGSDEYDSNVTCKNTCWEAG 126

BLAST of HG10005427 vs. ExPASy Swiss-Prot
Match: P14314 (Glucosidase 2 subunit beta OS=Homo sapiens OX=9606 GN=PRKCSH PE=1 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 1.0e-25
Identity = 47/86 (54.65%), Postives = 61/86 (70.93%), Query Frame = 0

Query: 41  YYSSEVIKCKDGSRSFAIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSS 100
           Y  S+   C DGS +   D++NDD+CDC DG+DEPGT+AC  G F+C N G  P +I S+
Sbjct: 31  YDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSN 90

Query: 101 RVNDRICDCCDGSDEYDGNIFCPNTC 127
           RVND +CDCCDG+DEY+  + C NTC
Sbjct: 91  RVNDGVCDCCDGTDEYNSGVICENTC 116

BLAST of HG10005427 vs. ExPASy Swiss-Prot
Match: O08795 (Glucosidase 2 subunit beta OS=Mus musculus OX=10090 GN=Prkcsh PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.0e-25
Identity = 47/86 (54.65%), Postives = 60/86 (69.77%), Query Frame = 0

Query: 41  YYSSEVIKCKDGSRSFAIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSS 100
           Y  S+   C DG+ +   D++NDD+CDC DG+DEPGT+AC  G F+C N G  P +I SS
Sbjct: 31  YEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSS 90

Query: 101 RVNDRICDCCDGSDEYDGNIFCPNTC 127
           RVND +CDCCDG+DEY+    C NTC
Sbjct: 91  RVNDGVCDCCDGTDEYNSGTVCENTC 116

BLAST of HG10005427 vs. ExPASy TrEMBL
Match: A0A6J1C765 (glucosidase 2 subunit beta OS=Momordica charantia OX=3673 GN=LOC111008003 PE=4 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 2.7e-101
Identity = 181/200 (90.50%), Postives = 190/200 (95.00%), Query Frame = 0

Query: 1   MEAPRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDR 60
           MEA R P STSFVS++SICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSF IDR
Sbjct: 1   MEAGRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+FIFSS VNDRICDCCDGSDEYDGNI
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALT 180
           FCPNTCVMGGNI YKSNN ISTT+DV +VNRKVKEEITKED+FQKL+GLKLVI+LQVALT
Sbjct: 121 FCPNTCVMGGNIAYKSNNYISTTQDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180

Query: 181 SFAVLIWVNRCRVKSKRRRH 201
           SFAV IWVNRCR +SKRRRH
Sbjct: 181 SFAVFIWVNRCRARSKRRRH 200

BLAST of HG10005427 vs. ExPASy TrEMBL
Match: A0A0A0K1G3 (PRKCSH-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041350 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.6e-98
Identity = 178/197 (90.36%), Postives = 184/197 (93.40%), Query Frame = 0

Query: 4   PRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDRLND 63
           PRSP STSF SLLSIC FF SVRS PS IGVHPLDEKYYSSEVIKCKDGSRSF IDRLND
Sbjct: 3   PRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLND 62

Query: 64  DFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNIFCP 123
           +FCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVND ICDCCDGSDEY+GNIFCP
Sbjct: 63  NFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCP 122

Query: 124 NTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALTSFA 183
           NTCVMGGN +YKS NDISTTRDVD+V RKVKEEITKED+FQKL GLKLVIILQVALTSFA
Sbjct: 123 NTCVMGGN-MYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTSFA 182

Query: 184 VLIWVNRCRVKSKRRRH 201
           +LIW NRCRVKSKRRRH
Sbjct: 183 ILIWANRCRVKSKRRRH 198

BLAST of HG10005427 vs. ExPASy TrEMBL
Match: A0A1S4E0C8 (glucosidase 2 subunit beta isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495629 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.8e-98
Identity = 179/200 (89.50%), Postives = 187/200 (93.50%), Query Frame = 0

Query: 1   MEAPRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDR 60
           MEA RSP STSFVSLLSIC FF SVR+ PS +GVHPLDEKYYSSEVIKCKDGSRSF IDR
Sbjct: 1   MEA-RSPKSTSFVSLLSICLFFTSVRAAPSFLGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVND ICDCCDGSDEY+GNI
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNI 120

Query: 121 FCPNTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALT 180
           FCPNTCVMGGN +YKSNND+STTRDVD+V RKVKEEITKED+FQKL GLKLVIILQVALT
Sbjct: 121 FCPNTCVMGGN-MYKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALT 180

Query: 181 SFAVLIWVNRCRVKSKRRRH 201
            FA+LIW NRCRVKSKRRRH
Sbjct: 181 IFAILIWANRCRVKSKRRRH 198

BLAST of HG10005427 vs. ExPASy TrEMBL
Match: A0A5D3BIQ3 (Glucosidase 2 subunit beta isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold786G00030 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.8e-98
Identity = 179/200 (89.50%), Postives = 187/200 (93.50%), Query Frame = 0

Query: 1   MEAPRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDR 60
           MEA RSP STSFVSLLSIC FF SVR+ PS +GVHPLDEKYYSSEVIKCKDGSRSF IDR
Sbjct: 1   MEA-RSPKSTSFVSLLSICLFFTSVRAAPSFLGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVND ICDCCDGSDEY+GNI
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNI 120

Query: 121 FCPNTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALT 180
           FCPNTCVMGGN +YKSNND+STTRDVD+V RKVKEEITKED+FQKL GLKLVIILQVALT
Sbjct: 121 FCPNTCVMGGN-MYKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALT 180

Query: 181 SFAVLIWVNRCRVKSKRRRH 201
            FA+LIW NRCRVKSKRRRH
Sbjct: 181 IFAILIWANRCRVKSKRRRH 198

BLAST of HG10005427 vs. ExPASy TrEMBL
Match: A0A6J1GLM3 (glucosidase 2 subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111455090 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 8.1e-98
Identity = 177/200 (88.50%), Postives = 184/200 (92.00%), Query Frame = 0

Query: 1   MEAPRSPNSTSFVSLLSICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDR 60
           MEA  SP S SFV LLS CFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSF IDR
Sbjct: 1   MEAGHSPKSMSFVLLLSFCFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRF+FSSRVND ICDCCDGSDEYDGNI
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFLFSSRVNDHICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLKLVIILQVALT 180
           FCPNTCVMGGN  YK++  IST+RDVDV+NRKVKEEITKED+F +LIGLKLVIILQVALT
Sbjct: 121 FCPNTCVMGGNAAYKNSYYISTSRDVDVINRKVKEEITKEDLFHRLIGLKLVIILQVALT 180

Query: 181 SFAVLIWVNRCRVKSKRRRH 201
            FAVLIW NRCR KSKRRRH
Sbjct: 181 CFAVLIWANRCRAKSKRRRH 200

BLAST of HG10005427 vs. TAIR 10
Match: AT2G42390.1 (protein kinase C substrate, heavy chain-related )

HSP 1 Score: 223.8 bits (569), Expect = 1.3e-58
Identity = 108/189 (57.14%), Postives = 137/189 (72.49%), Query Frame = 0

Query: 17  SICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFAIDRLNDDFCDCVDGTDEPG 76
           S+     SV S   L+GVHPLDEKY+ S+VIKCKDGS+SF  DRLND+FCDC+DGTDEPG
Sbjct: 11  SLAVVVISVASTSPLVGVHPLDEKYFDSDVIKCKDGSKSFTRDRLNDNFCDCLDGTDEPG 70

Query: 77  TSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNIFCPNTCVMGGNI--LY 136
           TSAC  GKFYCRN+GS+P+F++SSRVNDRICDCCDGSDEY+ +I CPNTC+MGGN+  +Y
Sbjct: 71  TSACPNGKFYCRNIGSSPKFVYSSRVNDRICDCCDGSDEYESSIHCPNTCIMGGNVNYIY 130

Query: 137 KSNNDISTTRDVDVVNRKVKEEIT---KEDMFQKLIGLKLVIILQVALTSFAVLIWVNRC 196
           K   ++ +           KE  T    +DM + L G+KLV  LQ+    F V++W+ R 
Sbjct: 131 KPRANLKSIHLQLGSTPHPKEFYTIGNLQDMVKNLQGMKLVFALQMVFIGFLVILWMLRS 190

Query: 197 RVKSKRRRH 201
           R +SKRRR+
Sbjct: 191 RARSKRRRY 199

BLAST of HG10005427 vs. TAIR 10
Match: AT5G56360.1 (calmodulin-binding protein )

HSP 1 Score: 157.1 bits (396), Expect = 1.4e-38
Identity = 82/161 (50.93%), Postives = 101/161 (62.73%), Query Frame = 0

Query: 11  SFVSLLSICFFFASVRSVPSLIGVHPLDEKYY-SSEVIKCKDGSRSFAIDRLNDDFCDCV 70
           SF+ LL+     +S       +G+ P DEKYY SS  IKCKDGS+ F   +LNDDFCDC 
Sbjct: 17  SFLLLLASAIRSSSSPPNDPFLGISPQDEKYYKSSSEIKCKDGSKKFTKAQLNDDFCDCS 76

Query: 71  DGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNIFCPNTCVMG 130
           DGTDEPGTSAC  GKFYCRN G +P  +FSSRVND ICDCCDGSDEYDG++ C NTC   
Sbjct: 77  DGTDEPGTSACPTGKFYCRNAGHSPVILFSSRVNDGICDCCDGSDEYDGHVSCQNTCWEA 136

Query: 131 GNILYKSNNDISTTRDVDVVNRKVKEEITKEDMFQKLIGLK 171
           G    ++      T +  +V R+ + E  K  + +    LK
Sbjct: 137 GKAARENLKKKIETYNQGLVIRRQEIEQAKVGLEKDAAELK 177

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887466.11.3e-10292.54glucosidase 2 subunit beta [Benincasa hispida][more]
XP_022136263.15.6e-10190.50glucosidase 2 subunit beta [Momordica charantia][more]
XP_004144515.17.5e-9890.36glucosidase 2 subunit beta [Cucumis sativus] >XP_031744257.1 glucosidase 2 subun... [more]
XP_008455474.19.8e-9889.50PREDICTED: glucosidase 2 subunit beta isoform X1 [Cucumis melo] >XP_016901681.1 ... [more]
XP_022952390.11.7e-9788.50glucosidase 2 subunit beta [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FM962.0e-3750.93Glucosidase 2 subunit beta OS=Arabidopsis thaliana OX=3702 GN=PSL4 PE=2 SV=1[more]
A2WNF53.5e-3762.07Glucosidase 2 subunit beta OS=Oryza sativa subsp. indica OX=39946 GN=OsI_01383 P... [more]
Q5NBP93.5e-3762.07Glucosidase 2 subunit beta OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0276... [more]
P143141.0e-2554.65Glucosidase 2 subunit beta OS=Homo sapiens OX=9606 GN=PRKCSH PE=1 SV=2[more]
O087951.0e-2554.65Glucosidase 2 subunit beta OS=Mus musculus OX=10090 GN=Prkcsh PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C7652.7e-10190.50glucosidase 2 subunit beta OS=Momordica charantia OX=3673 GN=LOC111008003 PE=4 S... [more]
A0A0A0K1G33.6e-9890.36PRKCSH-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041350... [more]
A0A1S4E0C84.8e-9889.50glucosidase 2 subunit beta isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495629 PE... [more]
A0A5D3BIQ34.8e-9889.50Glucosidase 2 subunit beta isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1GLM38.1e-9888.50glucosidase 2 subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111455090 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT2G42390.11.3e-5857.14protein kinase C substrate, heavy chain-related [more]
AT5G56360.11.4e-3850.93calmodulin-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR028146Glucosidase II beta subunit, N-terminalPFAMPF12999PRKCSH-likecoord: 13..132
e-value: 2.3E-32
score: 112.2
NoneNo IPR availablePANTHERPTHR12630:SF17EXPRESSED PROTEINcoord: 20..199
IPR039794Glucosidase II beta subunit-likePANTHERPTHR12630N-LINKED OLIGOSACCHARIDE PROCESSINGcoord: 20..199

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10005427.1HG10005427.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006491 N-glycan processing
cellular_component GO:0017177 glucosidase II complex
cellular_component GO:0016021 integral component of membrane