HG10005339 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10005339 vs. NCBI nr
Match: XP_038887715.1 (vacuolar sorting protein 18 isoform X1 [Benincasa hispida])

HSP 1 Score: 1767.7 bits (4577), Expect = 0.0e+00
Identity = 894/966 (92.55%), Postives = 909/966 (94.10%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           MEPGRPAFTVDLLERYAAKGRGVI+CMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV
Sbjct: 1   MEPGRPAFTVDLLERYAAKGRGVITCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPR+LTRLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLTRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFEL+ELPEAFMDLQMETTS+LN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM++YVMAVTPTRLYSFTG+GSLE                              QRRAIH
Sbjct: 181 GMRYYVMAVTPTRLYSFTGLGSLETVFSTYLERAVHFMELPGEIPNSELHFYIKQRRAIH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSGAGIYHGELNFGSQRS SNGDENFVENKALLDYSKLT+NSGT+KPSSMAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTLKPSSMAVSEFHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA                
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLDMKEYTAALANCRD+LQRDQVYLAQAEGALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTAALANCRDALQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFI ASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DIASDGHSTEYQS+IKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIASDGHSTEYQSVIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKP  P ELQYKFAPELIMLDAYETVESWM+TNNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGAPPELQYKFAPELIMLDAYETVESWMITNNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYAVIDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRD W+ASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDFWMASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISSMTTADKLRTQLDDAIAGECPFCGELMIREISLPFIS 918
           QITLLGGETRKD NG+FAEDSISSMT ADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRKDLNGSFAEDSISSMTPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

BLAST of HG10005339 vs. NCBI nr
Match: XP_004136806.1 (vacuolar sorting protein 18 [Cucumis sativus] >KGN43582.1 hypothetical protein Csa_020462 [Cucumis sativus])

HSP 1 Score: 1749.9 bits (4531), Expect = 0.0e+00
Identity = 891/968 (92.05%), Postives = 905/968 (93.49%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           ME GRPAFTVDLLERYAAKGRGVISCMAAGNDVI+LGTSKGWVTRYDFGVGDSIDFDLSV
Sbjct: 1   MEQGRPAFTVDLLERYAAKGRGVISCMAAGNDVIMLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPG+QSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGDQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLARLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFEL+ELPEAFMDLQMETTSILN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSILN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM++YVMAVTPTRLYSFTG GSLE                              QRRAIH
Sbjct: 181 GMRYYVMAVTPTRLYSFTGTGSLETVFSTYLERAVHFMELPGEIPNSELHFYIKQRRAIH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSGAGIYHGELNFGSQRS SNGDENFVENKALLDYSKLT+NSGTVKPSSMAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTVKPSSMAVSEFHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRISEQIIEELQFDQTSEAI+RGILGLCSDATA                
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAITRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAE ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEDALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFI ASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           D A DGHSTEYQSII+EFRAFLSD KDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DTAFDGHSTEYQSIIQEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKP VPAELQYKFAPELIMLDAYETVESWM+TNNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPAELQYKFAPELIMLDAYETVESWMITNNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAIC+SLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYAVIDRDEDCGVCK
Sbjct: 781 IDDFKEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDLW+ SSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDLWMTSSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISS--MTTADKLRTQLDDAIAGECPFCGELMIREISLPF 918
           QITLLGGETRKDSNG+FAEDSISS  MT ADKLRTQLDDAIAGECPFCGELMIREISLPF
Sbjct: 901 QITLLGGETRKDSNGSFAEDSISSMTMTPADKLRTQLDDAIAGECPFCGELMIREISLPF 960

BLAST of HG10005339 vs. NCBI nr
Match: KAA0031599.1 (vacuolar protein sorting-associated protein 18-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1744.9 bits (4518), Expect = 0.0e+00
Identity = 890/980 (90.82%), Postives = 905/980 (92.35%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           ME GRPAFTVDLLERYAAKGRGVISCMAAGNDVI+LGTSKGWVTRYDFGVGDSIDFDLSV
Sbjct: 1   MEQGRPAFTVDLLERYAAKGRGVISCMAAGNDVIMLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLARLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFEL+ELPEAFMDLQMETT++LN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTNVLN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------------ 240
           GM++YVMAVTPTRLYSFTG GSLE                                    
Sbjct: 181 GMRYYVMAVTPTRLYSFTGTGSLETVFSTYLERAVHFMELPGEIPNRQCYQFRRLIHYSE 240

Query: 241 ------QRRAIHFAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTV 300
                 QRRAIHFAWLSGAGIYHGELNFGSQRS SNGDENFVENKALLDYSKLT+NSGTV
Sbjct: 241 LHFYIKQRRAIHFAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTV 300

Query: 301 KPSSMAVSEFHFLLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---- 360
           KPSSMAVSEFHFLLLIGNKVKVVNRISEQIIEELQFDQTSEAI+RGILGLCSDATA    
Sbjct: 301 KPSSMAVSEFHFLLLIGNKVKVVNRISEQIIEELQFDQTSEAITRGILGLCSDATAGLFY 360

Query: 361 ---------------GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYL 420
                          GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAE ALASRDYL
Sbjct: 361 AYDQNSIFQVSVNDEGRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEDALASRDYL 420

Query: 421 RAASFYAKINYILSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATEL 480
           RAASFYAKINYILSFEEITLKFI A EQDALRTFLLRKLDNLTKDDKCQITMISTWATEL
Sbjct: 421 RAASFYAKINYILSFEEITLKFISAGEQDALRTFLLRKLDNLTKDDKCQITMISTWATEL 480

Query: 481 YLDKINRLLLDDDIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELV 540
           YLDKINRLLLDDD A DGHSTEYQSII+EFRAFLSD KDVLDEVTTMKLLESYGRVEELV
Sbjct: 481 YLDKINRLLLDDDTAFDGHSTEYQSIIQEFRAFLSDSKDVLDEVTTMKLLESYGRVEELV 540

Query: 541 FFAGLKEQYEIVVHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMM 600
           FFAGLKEQYEIVVHHYIQQGEAKKALEVLQKP VPAELQYKFAPELIMLDAYETVESWM+
Sbjct: 541 FFAGLKEQYEIVVHHYIQQGEAKKALEVLQKPGVPAELQYKFAPELIMLDAYETVESWMI 600

Query: 601 TNNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQED 660
           TNNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQED
Sbjct: 601 TNNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQED 660

Query: 661 DSALLRFLQCKFGKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALAL 720
           DSALLRFLQCKFGKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALAL
Sbjct: 661 DSALLRFLQCKFGKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALAL 720

Query: 721 QVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKI 780
           QVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKI
Sbjct: 721 QVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKI 780

Query: 781 EDILPFFPDFALIDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYA 840
           EDILPFFPDFALIDDFKEAIC+SLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYA
Sbjct: 781 EDILPFFPDFALIDDFKEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYA 840

Query: 841 VIDRDEDCGVCKRKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTD 900
           VIDRDEDCGVCKRKILTVGRDLW+ASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTD
Sbjct: 841 VIDRDEDCGVCKRKILTVGRDLWMASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTD 900

Query: 901 EAQAEYILDLQKQITLLGGETRKDSNGNFAEDSISSMT--TADKLRTQLDDAIAGECPFC 918
           EAQAEYILDLQKQITLLGGETRKDSNG+FAEDSISSMT   ADKLRTQLDDAIAGECPFC
Sbjct: 901 EAQAEYILDLQKQITLLGGETRKDSNGSFAEDSISSMTMSPADKLRTQLDDAIAGECPFC 960

BLAST of HG10005339 vs. NCBI nr
Match: XP_008455352.1 (PREDICTED: LOW QUALITY PROTEIN: vacuolar protein sorting-associated protein 18 homolog [Cucumis melo])

HSP 1 Score: 1741.9 bits (4510), Expect = 0.0e+00
Identity = 887/968 (91.63%), Postives = 903/968 (93.29%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           ME GRPAFTVDLLERYAAKGRGVISCMAAGNDVI+LGTSKGWVTRYDFGVGDSIDFDLSV
Sbjct: 1   MEQGRPAFTVDLLERYAAKGRGVISCMAAGNDVIMLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLARLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEK KKEKYVKFLFEL+ELPEAFMDLQMETT++LN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKXKKEKYVKFLFELAELPEAFMDLQMETTNVLN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM++YVMAVTPTRLYSFTG GSLE                              QRRAIH
Sbjct: 181 GMRYYVMAVTPTRLYSFTGTGSLETVFSTYLERAVHFMELPGEIPNSELHFYIKQRRAIH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSGAGIYHGELNFGSQRS SNGDENFVENKALLDYSKLT+NSGTVKPSSMAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTVKPSSMAVSEFHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRISEQIIEELQFDQTSEAI+RGILGLCSDATA                
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAITRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAE ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEDALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEI+LKFI A EQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEISLKFISAGEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           D A DGHSTEYQSII+EFRAFLSD KDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DTAFDGHSTEYQSIIQEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKP V AELQYKFAPELIMLDAYETVESWM+TNNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVLAELQYKFAPELIMLDAYETVESWMITNNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAIC+SLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYAVIDRDEDCGVCK
Sbjct: 781 IDDFKEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDLW+ASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDLWMASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISSMT--TADKLRTQLDDAIAGECPFCGELMIREISLPF 918
           QITLLGGETRKDSNG+FAEDSISSMT   ADKLRTQLDDAIAGECPFCGELMIREISLPF
Sbjct: 901 QITLLGGETRKDSNGSFAEDSISSMTMSPADKLRTQLDDAIAGECPFCGELMIREISLPF 960

BLAST of HG10005339 vs. NCBI nr
Match: XP_022968943.1 (vacuolar protein sorting-associated protein 18 homolog [Cucurbita maxima])

HSP 1 Score: 1731.5 bits (4483), Expect = 0.0e+00
Identity = 872/966 (90.27%), Postives = 903/966 (93.48%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           M+PGRPAFTVDLLERYAAKGRGVISCM+AGNDVILLGTSKGWVTRYDFG+GDSID+DLSV
Sbjct: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPGEQSIHR+FVDPGGSHCITTIVG  GADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRIFVDPGGSHCITTIVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFEL+ELPEAFMDLQMETTS+LN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM+FYVMAVTPTRLYSFTGIGSLE                              QRRA+H
Sbjct: 181 GMRFYVMAVTPTRLYSFTGIGSLETVFSTYLERAVHFMELPGDIPNSELHFYIKQRRAMH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSGAGIYHGELNFGSQ SSSNGDENFVENKALLDYSKLT+NSG+VKPSS+AVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRI+EQI+EELQFDQT+EAI RGILGLCSDATA                
Sbjct: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLDMKEYTA+LA+CRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEI LKFI A+EQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEIALKFISANEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DI SDGHST+YQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIVSDGHSTDYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKP VPA+LQYKFAPELIMLDAYETVESWM+T+NLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKG ENGP+FFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYA+IDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRD  +ASSYTSVA MAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISSMTTADKLRTQLDDAIAGECPFCGELMIREISLPFIS 918
           QITLLGGETR++SNG+FAEDSISS+T ADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

BLAST of HG10005339 vs. ExPASy Swiss-Prot
Match: F4IDS7 (Vacuolar sorting protein 18 OS=Arabidopsis thaliana OX=3702 GN=VPS18 PE=1 SV=1)

HSP 1 Score: 1483.4 bits (3839), Expect = 0.0e+00
Identity = 735/967 (76.01%), Postives = 818/967 (84.59%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           M+ GR  F+VDLLERYA K RG+I+CMAAGNDVI+LGTSKGW+ RYDFGVG S D DL+V
Sbjct: 1   MDQGRQVFSVDLLERYATKNRGMITCMAAGNDVIVLGTSKGWIIRYDFGVGSSNDIDLAV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GR GEQSIH+VFVDPGGSHCI T+ G GGA+TFY HAKW KPRVL+RLKGL+VN VAWNR
Sbjct: 61  GRTGEQSIHKVFVDPGGSHCIATVTGVGGAETFYTHAKWLKPRVLSRLKGLLVNAVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           Q ITE STKE+ILGT +GQLFE+AVDEK+K+EKY+KFLFEL ELPEAF  LQMET +I +
Sbjct: 121 QQITEVSTKEIILGTQDGQLFEMAVDEKDKREKYIKFLFELEELPEAFKALQMETANISS 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM++YVMAVTPTRLYSFTGIG+LE                              QRRA+H
Sbjct: 181 GMRYYVMAVTPTRLYSFTGIGTLESVFASYKERAVHFMELPGEIPNSELHFFIKQRRAVH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSG GIYHG LNFG+Q S  NGDENFVENKALLDYSKL+D +  VKP SMA+SE+HF
Sbjct: 241 FAWLSGTGIYHGGLNFGAQHSYPNGDENFVENKALLDYSKLSDGTEAVKPGSMALSEYHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRISEQIIEELQFD TS+++SRGI+GLCSDA+A                
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDITSDSVSRGIIGLCSDASANVFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLD+K Y AALANCRD LQRDQVYL QAE A   ++YLRAASFYAKINY+
Sbjct: 361 IDEGRDMWKVYLDLKVYAAALANCRDPLQRDQVYLVQAESAFTDKEYLRAASFYAKINYV 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           +SFEE+TLKFI  +E +ALRTFLL KLDNL+KDDKCQITMISTWATELYLDKINRLLL+D
Sbjct: 421 ISFEEVTLKFISINEPEALRTFLLHKLDNLSKDDKCQITMISTWATELYLDKINRLLLED 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           D A +   +EY S+I+EFRAF+SDCKD LDE TT+K+LESYGRVEELV+FA LKEQYEIV
Sbjct: 481 DTAIENRDSEYHSVIQEFRAFMSDCKDELDEATTVKILESYGRVEELVYFANLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           V HYIQQGEAKKALEVLQK  V  ELQY+FAPELIMLDAYETVESWM   NLNPR+LI A
Sbjct: 541 VLHYIQQGEAKKALEVLQKSSVSVELQYQFAPELIMLDAYETVESWMANKNLNPRRLITA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYS  PHAKNETHEVIKYLE+CVHRLHNEDPG+H+LLLSLYAKQEDD ALLRFLQCKF
Sbjct: 601 MMRYSSGPHAKNETHEVIKYLEFCVHRLHNEDPGIHSLLLSLYAKQEDDGALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKG+ENGPEFFYDPKYALRLCLKE+R RACVHIYSMM+MHEEAVALALQ+D ELAMAEAD
Sbjct: 661 GKGRENGPEFFYDPKYALRLCLKERRTRACVHIYSMMSMHEEAVALALQIDPELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLM+AKHV++ EKG KRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMVAKHVVKQEKGAKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQI+QLK+EMNDAT GADNIR DISAL QRYAVIDRDE+CGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIEQLKEEMNDATRGADNIRNDISALTQRYAVIDRDEECGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKIL +  D  +A  Y+S   +APFYVFPCGH FHAQCLI HVT C  E QAE+ILDLQK
Sbjct: 841 RKILMMSGDFRMAQGYSSAGPLAPFYVFPCGHSFHAQCLITHVTSCAHEEQAEHILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISSMTTADKLRTQLDDAIAGECPFCGELMIREISLPFIS 919
           Q+TLLG ETR+D NGN +++ I+S TTADKLR++LDDAIA ECPFCGELMI EI+LPFI 
Sbjct: 901 QLTLLGSETRRDINGNRSDEPITSTTTADKLRSELDDAIASECPFCGELMINEITLPFIK 960

BLAST of HG10005339 vs. ExPASy Swiss-Prot
Match: P59015 (Vacuolar protein sorting-associated protein 18 homolog OS=Danio rerio OX=7955 GN=vps18 PE=2 SV=2)

HSP 1 Score: 485.7 bits (1249), Expect = 1.2e-135
Identity = 330/969 (34.06%), Postives = 518/969 (53.46%), Query Frame = 0

Query: 26  CMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSVGRPGEQSIHRVFVDPGGSH---CIT 85
           CM+ G D +L         R D G  D  +  + +GR  +  +HR+F+DP GSH   C+T
Sbjct: 68  CMSLGKDTLL---------RIDLGKPDQPN-QIELGRKDDSKVHRLFLDPTGSHLVICLT 127

Query: 86  TIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNRQHITEASTKEVILGTDNGQLFE 145
           T       +  Y++    K R L+R +G ++ ++ WN+   +E +T  +++GT  G +FE
Sbjct: 128 T------NECVYLNRNTQKVRGLSRWRGHLIESIGWNKLIGSETNTGPILVGTSQGIIFE 187

Query: 146 LAVDEKE------KKEKYVKFLFELSE--LPEAFMDLQMETTSILNGM--KFYVMAVTPT 205
             +   E        ++Y + +  L E   P     L++E      G+  K++++A T  
Sbjct: 188 AEISASEGSLFNTNPDQYFRQVHYLEEDGKPAPVCCLEVE-----RGLETKYFIIATTRK 247

Query: 206 RLYSFTG---IGSLEQ------------------------------------RRAIHFAW 265
           RL+ F G    GS +Q                                     R   FAW
Sbjct: 248 RLFQFVGKLAEGSEQQGFSSIFAQNQDLLPSFQEFPVNMGYSEITFYTSKLRSRPKTFAW 307

Query: 266 LSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHFLLL 325
           + G G+ +G+L++          ++ + +  + +Y++  D    VKP S+ +++FHFLLL
Sbjct: 308 MMGNGVLYGQLDYVR-------PDSLLSDVQVWEYTQDID-LNFVKPISIVLTQFHFLLL 367

Query: 326 IGNKVKVVNRISEQIIEE----LQFDQTSEAISRGILGLCSDAT-----------AGRDM 385
           + ++V+ +  ++ Q++ E     +F    + I   I GL    T             RD+
Sbjct: 368 LPDRVRGICTLNGQVVHEDVFPEKFGTLQKMIKDPITGLVWIYTEKAVFRYHIQKEARDV 427

Query: 386 WKVYLDMKEYTAALANCRDSLQ-RDQVYLAQAEGALASRDYLRAASFYAKI-NYILSFEE 445
           W++Y++M ++  A   C+D  +  D V   +AE    ++ YL +A  YA   NY   FEE
Sbjct: 428 WQMYMNMNKFDLAKEYCKDRPECLDMVLAKEAEHCFQNKRYLESAKCYALTQNY---FEE 487

Query: 446 ITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDDDIASD 505
           I LKFI A +++AL+ FL++KL NL   +K QIT++ TW TELYL+++ +L  D     +
Sbjct: 488 IALKFIEAKQEEALKEFLIKKLVNLKPSEKTQITLLVTWLTELYLNRLGQLEAD-----E 547

Query: 506 GHSTEYQSIIKEFRAFLSDCKD----VLDEVTTMKLLESYGRVEELVFFAGLKEQYEIVV 565
           G    +    +EFR FL   K       +  T   LL S+G V+ +V+F+ + + YE V+
Sbjct: 548 GKQHLFLETREEFRTFLKSPKHKDCFYNNRSTIYDLLASHGDVDNMVYFSVIMQDYERVI 607

Query: 566 HHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWM-MTNNLNPRKLIPA 625
            HY Q  +   AL+VL K     +L YKF+P L+     + V++W+ M N L+P+ LIPA
Sbjct: 608 SHYCQHDDYSAALDVLSK-HCDDKLFYKFSPVLMQHIPKKVVDAWIQMGNRLDPKNLIPA 667

Query: 626 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 685
           ++ YS +  +  + +E I+Y+E+CV+ L  ++  +HN LLSLYAK + D ALL +L+   
Sbjct: 668 LVNYS-QMGSMQQINETIRYMEFCVYELDVKEEAIHNYLLSLYAKHKPD-ALLWYLE--- 727

Query: 686 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 745
            +   +  +  YD KYALRLC +   ++ACV +Y +M ++EEAV LAL+VD +LA + AD
Sbjct: 728 -QAGTHVSDIHYDLKYALRLCSEHGYLQACVLVYKIMELYEEAVDLALKVDVDLAKSCAD 787

Query: 746 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 805
             EDDE+LRKKLWL IA+HV++ EK     +++KA+  L   + LLKIEDILPFFPDF  
Sbjct: 788 LPEDDEELRKKLWLKIARHVVQEEK-----DVKKAMNCLSSCN-LLKIEDILPFFPDFVT 847

Query: 806 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 865
           ID FKEAICSSLE+YNK I++LKQEM +AT  A  IR+DI  +  +Y V++  E C  C 
Sbjct: 848 IDHFKEAICSSLEEYNKHIEELKQEMEEATESAKRIREDIQEMRNKYGVVESQEKCATCD 907

Query: 866 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 918
             +L                   PFY+F CGH FH  CL+  V       +   + +LQK
Sbjct: 908 FPLLN-----------------RPFYLFLCGHMFHYDCLLQEVIPHLSVYKQNKLDELQK 967

BLAST of HG10005339 vs. ExPASy Swiss-Prot
Match: Q8R307 (Vacuolar protein sorting-associated protein 18 homolog OS=Mus musculus OX=10090 GN=Vps18 PE=1 SV=2)

HSP 1 Score: 443.4 bits (1139), Expect = 6.6e-123
Identity = 314/966 (32.51%), Postives = 486/966 (50.31%), Query Frame = 0

Query: 26  CMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSVGRPGEQSIHRVFVDPGGSHCITTIV 85
           CM+ G D +L         R D G     +  + +GR  +  +H++F+D  GSH +  + 
Sbjct: 68  CMSLGKDTLL---------RIDLGKASEPN-RVELGRKDDAKVHKMFLDHTGSHLLVALS 127

Query: 86  GTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNRQHITEASTKEVILGTDNGQLFELAV 145
            T   +  YM+    K R L R KG +V +V WN+    E+ST  +++GT  GQ+FE  +
Sbjct: 128 ST---EVLYMNRNGQKARPLARWKGQLVESVGWNKAMGNESSTGPILVGTAQGQIFEAEL 187

Query: 146 DEKE------KKEKYVKFLFELSEL--PEAFMDLQMETTSILNGMKFYVMAVTPTRLYSF 205
              E        + Y + L+ L+E   P     L+ E      G   +V+A T  RL+ F
Sbjct: 188 SASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRG---FVIATTRQRLFQF 247

Query: 206 TG-------------------------------IGSLE--------QRRAIHFAWLSGAG 265
            G                               +G  E        +     FAW+ G G
Sbjct: 248 IGRAVEDTEAQGFAGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDG 307

Query: 266 IYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHFLLLIGNKV 325
           + +G L+ G         ++ +  + + +Y       G   P ++ +++FHFLLL+ ++V
Sbjct: 308 VLYGSLDCGR-------PDSLLSEERVWEYPAGV-GPGANPPLAIVLTQFHFLLLLADRV 367

Query: 326 KVVNRISEQIIEELQFDQTSEAI-------SRGILGLCSDATA--------GRDMWKVYL 385
           + V  ++ Q++    F +    +       S G L   ++            RD+W+ YL
Sbjct: 368 EAVCTLTGQVVLRDHFLEKFGPLRHMVKDSSTGHLWAYTERAVFRYHVQREARDVWRTYL 427

Query: 386 DMKEYTAALANCRDSLQ-RDQVYLAQAEGALASRDYLRAASFYAKINYILSFEEITLKFI 445
           DM  +  A   CR+     D V   +A+       YL +A  YA       FEEI LKF+
Sbjct: 428 DMNRFDLAKEYCRERPDCLDTVLAREADFCFRQHRYLESARCYALTQSY--FEEIALKFL 487

Query: 446 GASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDDDIASDGHSTEY 505
            A +++AL  FL RKL  L   ++ Q T+++TW TELYL ++  L  D D       T Y
Sbjct: 488 EARQEEALAEFLQRKLAGLKPTERTQATLLTTWLTELYLSRLGALQGDPDAL-----TLY 547

Query: 506 QSIIKEFRAFLSDCKD----VLDEVTTMKLLESYGRVEELVFFAGLKEQYEIVVHHYIQQ 565
           +   + FR FLS  +          +  +LL S+G  E +V+FA + + YE VV ++ Q 
Sbjct: 548 RDTRECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQH 607

Query: 566 GEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWM-MTNNLNPRKLIPAMMRYSG 625
              ++AL VL + R P +L YKF+P LI     + V++W+ M + L+ R+LIPA++ YS 
Sbjct: 608 EAYEEALAVLARHRDP-QLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQ 667

Query: 626 EPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKFGKGQEN 685
              A+ +  + I+Y+E+CV+ L   +  +HN LLSLYA+ +  ++LL +L+    +   +
Sbjct: 668 GGEAQ-QVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGQ-PASLLAYLE----QAGAS 727

Query: 686 GPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEADKVEDDE 745
                YD KYALRLC +    RACVH+Y ++ ++EEAV LALQVD +LA   AD  E+DE
Sbjct: 728 PHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDE 787

Query: 746 DLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFALIDDFKE 805
           +LRKKLWL IA+HV++ E     E+++ A+A L     LLKIED+LPFFPDF  ID FKE
Sbjct: 788 ELRKKLWLKIARHVVQEE-----EDVQTAMACLASCP-LLKIEDVLPFFPDFVTIDHFKE 847

Query: 806 AICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCKRKILTV 865
           AICSSL+ YN  I +L++EM +AT  A  IR+D+  L  RY  ++  + C  C   +L  
Sbjct: 848 AICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCSTCDFPLLN- 907

Query: 866 GRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQKQITLLG 923
                            PFY+F CGH FHA CL+  V       +   + +LQ+++    
Sbjct: 908 ----------------RPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAP 967

BLAST of HG10005339 vs. ExPASy Swiss-Prot
Match: Q9P253 (Vacuolar protein sorting-associated protein 18 homolog OS=Homo sapiens OX=9606 GN=VPS18 PE=1 SV=2)

HSP 1 Score: 441.8 bits (1135), Expect = 1.9e-122
Identity = 312/964 (32.37%), Postives = 486/964 (50.41%), Query Frame = 0

Query: 26  CMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSVGRPGEQSIHRVFVDPGGSHCITTIV 85
           CM+ G D +L         R D G  +  +  + +GR  +  +H++F+D  GSH +  + 
Sbjct: 68  CMSLGKDTLL---------RIDLGKANEPN-HVELGRKDDAKVHKMFLDHTGSHLLIALS 127

Query: 86  GTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNRQHITEASTKEVILGTDNGQLFELAV 145
            T   +  Y++    K R L R KG +V +V WN+   TE+ST  +++GT  G +FE  +
Sbjct: 128 ST---EVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAEL 187

Query: 146 DEKE------KKEKYVKFLFELSELPEAFMDLQMETTSILNGMKFYVMAVTPTRLYSFTG 205
              E        + Y + L+ L+E         +E     +G  F V+A T  RL+ F G
Sbjct: 188 SASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSF-VIATTRQRLFQFIG 247

Query: 206 -------------------------------IGSLE--------QRRAIHFAWLSGAGIY 265
                                          +G  E        +     FAW+ G G+ 
Sbjct: 248 RAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVL 307

Query: 266 HGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHFLLLIGNKVKV 325
           +G L+ G         ++ +  + + +Y +     G   P ++ +++FHFLLL+ ++V+ 
Sbjct: 308 YGALDCGR-------PDSLLSEERVWEYPEGV-GPGASPPLAIVLTQFHFLLLLADRVEA 367

Query: 326 VNRISEQIIEELQFDQTSEAI-------SRGILGLCSDATA--------GRDMWKVYLDM 385
           V  ++ Q++    F +    +       S G L   ++            RD+W+ YLDM
Sbjct: 368 VCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDM 427

Query: 386 KEYTAALANCRDSLQ-RDQVYLAQAEGALASRDYLRAASFYAKINYILSFEEITLKFIGA 445
             +  A   CR+     D V   +A+     R YL +A  YA       FEEI LKF+ A
Sbjct: 428 NRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSY--FEEIALKFLEA 487

Query: 446 SEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDDDIASDGHSTEYQS 505
            +++AL  FL RKL +L   ++ Q T+++TW TELYL ++  L  D +       T Y+ 
Sbjct: 488 RQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEAL-----TLYRE 547

Query: 506 IIKEFRAFLSDCKD----VLDEVTTMKLLESYGRVEELVFFAGLKEQYEIVVHHYIQQGE 565
             + FR FLS  +          +  +LL S+G  E +V+FA + + YE VV ++ Q   
Sbjct: 548 TKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEA 607

Query: 566 AKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWM-MTNNLNPRKLIPAMMRYSGEP 625
            ++AL VL + R P +L YKF+P LI     + V++W+ M + L+ R+LIPA++ YS + 
Sbjct: 608 YEEALAVLARHRDP-QLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYS-QG 667

Query: 626 HAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKFGKGQENGP 685
               +  + I+Y+E+CV+ L   +  +HN LLSLYA+   DS LL +L+    +   +  
Sbjct: 668 GEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDS-LLAYLE----QAGASPH 727

Query: 686 EFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEADKVEDDEDL 745
              YD KYALRLC +    RACVH+Y ++ ++EEAV LALQVD +LA   AD  E+DE+L
Sbjct: 728 RVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEEL 787

Query: 746 RKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFALIDDFKEAI 805
           RKKLWL IA+HV++ E     E+++ A+A L     LLKIED+LPFFPDF  ID FKEAI
Sbjct: 788 RKKLWLKIARHVVQEE-----EDVQTAMACLASCP-LLKIEDVLPFFPDFVTIDHFKEAI 847

Query: 806 CSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCKRKILTVGR 865
           CSSL+ YN  I +L++EM +AT  A  IR+D+  L  RY  ++  + C  C   +L    
Sbjct: 848 CSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLN--- 907

Query: 866 DLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQKQITLLGGE 923
                          PFY+F CGH FHA CL+  V       +   + +LQ+++      
Sbjct: 908 --------------RPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPP 967

BLAST of HG10005339 vs. ExPASy Swiss-Prot
Match: Q24314 (Vacuolar protein sorting-associated protein 18 homolog OS=Drosophila melanogaster OX=7227 GN=dor PE=1 SV=3)

HSP 1 Score: 296.2 bits (757), Expect = 1.3e-78
Identity = 259/934 (27.73%), Postives = 431/934 (46.15%), Query Frame = 0

Query: 68  IHRVFVDPGGSHCITTIV---GTGGA--DTFYMH------AKWSKPRVLTRLKGLVVNTV 127
           I R+F+DP G H I  +V    T G   D  Y+H      A+  K R + + K   +  V
Sbjct: 111 ITRMFLDPTGHHIIIALVPKSATAGVSPDFLYIHCLESPQAQQLKVRRIEKFKDHEITAV 170

Query: 128 AWNRQHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETT 187
           A+N  H  E+ST  ++LGT  G +FE  ++         K L++L      +    ++  
Sbjct: 171 AFNPYHGNESSTGPILLGTSRGLIFETELNPAADGHVQRKQLYDLGLGRPKYPITGLKLL 230

Query: 188 SILNGMKFYVMAVTPTRLYSFTGIGSLEQR--RAI------------------------- 247
            + N  ++ ++  +P  +Y+F      E+R  +AI                         
Sbjct: 231 RVPNSSRYIIVVTSPECIYTFQETLKAEERSLQAIFAGYVSGVQEPHCEERKTDLTFSQL 290

Query: 248 ------------HFAWLSGAGIYHGELNFGSQRSSSNGDENFVENKAL-LDYSKLTDNSG 307
                        +AWL G GI  GEL+       +N     + N  + LD+ K    S 
Sbjct: 291 RFFAPPNSKYPKQWAWLCGEGIRVGELSI-----EANSAATLIGNTLINLDFEKTMHLSY 350

Query: 308 TVK----PSSMAVSEFHFLLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDA 367
             +    P +  ++E+H +LL  + V+ +  ++++ + +  FD   EA     L +  D 
Sbjct: 351 GERRLNTPKAFVLTEYHAVLLYADHVRAICLLNQEQVYQEAFD---EARVGKPLSIERDE 410

Query: 368 TAG-------------------RDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQ-AEGA 427
             G                   R++W++YLD  +Y  A A+  +  +  Q+ L Q A+ A
Sbjct: 411 LTGSIYVYTVKTVFNLRVTREERNVWRIYLDKGQYELATAHAAEDPEHLQLVLCQRADAA 470

Query: 428 LASRDYLRAASFYAKINYILSFEEITLKFIGASEQDALRTFLLRKL----------DNLT 487
            A   Y  AA +YA+ +   SFEE+ LKF+   ++  +  ++ ++L          D L 
Sbjct: 471 FADGSYQVAADYYAETD--KSFEEVCLKFMVLPDKRPIINYVKKRLSRVTTKPMETDELD 530

Query: 488 KDDKCQITMISTWATELYLDKINRLLLDDDIASDGHSTEYQSIIKEFRAFLSDCKDVLDE 547
           +D    I  +  W  +LYL +IN    D++  S    TEY   + E  A +  C     E
Sbjct: 531 EDKMNIIKALVIWLIDLYLIQINMPDKDEEWRS-SWQTEYDEFMME--AHVLSCTRQNRE 590

Query: 548 VTTMKLLESYGRVEELVFFAGLKEQYEIVVHHYIQQGEAKKALEVLQKPRVPAELQYKFA 607
            T  +L+  +     +  FA     Y+ VV   ++     +AL+ L   R P EL YK+A
Sbjct: 591 -TVRQLIAEHADPRNMAQFAIAIGDYDEVVAQQLKAECYAEALQTLINQRNP-ELFYKYA 650

Query: 608 PELIMLDAYETVESWM-MTNNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHN 667
           PELI      TV++ M   + L   KL+P ++        + +  +  +YLE+ +++L+ 
Sbjct: 651 PELITRLPKPTVDALMAQGSRLEVEKLVPTLIIMEN----REQREQTQRYLEFAIYKLNT 710

Query: 668 EDPGVHNLLLSLYAKQEDDSALLRFLQCKFGKGQENGPEFFYDPKYALRLCLKEKRMRAC 727
            +  +HN LL LYA+ E    L+++L+    +G++      YD  YA ++C       A 
Sbjct: 711 TNDAIHNFLLHLYAEHE-PKLLMKYLEI---QGRDES-LVHYDIYYAHKVCTDLDVKEAR 770

Query: 728 VHIYSMMAMHEEAVALALQVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRE 787
           V +  M+     AV LAL  D +LA   A +  D + +R+KLWL IA H I   KGT   
Sbjct: 771 VFLECMLRKWISAVDLALTFDMKLAKETASRPSDSK-IRRKLWLRIAYHDI---KGT--N 830

Query: 788 NIRKAIAFLKETDGLLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIDQLKQEMNDAT 847
           +++KA+  LKE D LL+IED+LPFF DF  ID+FKEAIC +L DYN++I +L++EM + T
Sbjct: 831 DVKKALNLLKECD-LLRIEDLLPFFADFEKIDNFKEAICDALRDYNQRIQELQREMAETT 890

Query: 848 HGADNIRKDISALAQRYAVIDRDEDCGVCKRKILTVGRDLWLASSYTSVAHMAPFYVFPC 907
              D +  ++  L Q    ++  + C +C+  +L                 + PF++F C
Sbjct: 891 EQTDRVTAELQQLRQHSLTVESQDTCEICEMMLL-----------------VKPFFIFIC 950

Query: 908 GHGFHAQCLIAHVTRCTDEAQAEYILDLQKQITL-LGGETRKDSNGNFAEDSISSMTTAD 915
           GH FH+ CL  HV     + Q   +  L++Q+   +  + +  S     + ++       
Sbjct: 951 GHKFHSDCLEKHVVPLLTKEQCRRLGTLKQQLEAEVQTQAQPQSGALSKQQAMELQRKRA 995

BLAST of HG10005339 vs. ExPASy TrEMBL
Match: A0A0A0K5N6 (Pep3_Vps18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G046700 PE=3 SV=1)

HSP 1 Score: 1749.9 bits (4531), Expect = 0.0e+00
Identity = 891/968 (92.05%), Postives = 905/968 (93.49%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           ME GRPAFTVDLLERYAAKGRGVISCMAAGNDVI+LGTSKGWVTRYDFGVGDSIDFDLSV
Sbjct: 1   MEQGRPAFTVDLLERYAAKGRGVISCMAAGNDVIMLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPG+QSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGDQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLARLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFEL+ELPEAFMDLQMETTSILN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSILN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM++YVMAVTPTRLYSFTG GSLE                              QRRAIH
Sbjct: 181 GMRYYVMAVTPTRLYSFTGTGSLETVFSTYLERAVHFMELPGEIPNSELHFYIKQRRAIH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSGAGIYHGELNFGSQRS SNGDENFVENKALLDYSKLT+NSGTVKPSSMAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTVKPSSMAVSEFHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRISEQIIEELQFDQTSEAI+RGILGLCSDATA                
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAITRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAE ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEDALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFI ASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           D A DGHSTEYQSII+EFRAFLSD KDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DTAFDGHSTEYQSIIQEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKP VPAELQYKFAPELIMLDAYETVESWM+TNNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPAELQYKFAPELIMLDAYETVESWMITNNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAIC+SLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYAVIDRDEDCGVCK
Sbjct: 781 IDDFKEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDLW+ SSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDLWMTSSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISS--MTTADKLRTQLDDAIAGECPFCGELMIREISLPF 918
           QITLLGGETRKDSNG+FAEDSISS  MT ADKLRTQLDDAIAGECPFCGELMIREISLPF
Sbjct: 901 QITLLGGETRKDSNGSFAEDSISSMTMTPADKLRTQLDDAIAGECPFCGELMIREISLPF 960

BLAST of HG10005339 vs. ExPASy TrEMBL
Match: A0A5A7SQQ7 (Vacuolar protein sorting-associated protein 18-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003670 PE=3 SV=1)

HSP 1 Score: 1744.9 bits (4518), Expect = 0.0e+00
Identity = 890/980 (90.82%), Postives = 905/980 (92.35%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           ME GRPAFTVDLLERYAAKGRGVISCMAAGNDVI+LGTSKGWVTRYDFGVGDSIDFDLSV
Sbjct: 1   MEQGRPAFTVDLLERYAAKGRGVISCMAAGNDVIMLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLARLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFEL+ELPEAFMDLQMETT++LN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTNVLN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------------ 240
           GM++YVMAVTPTRLYSFTG GSLE                                    
Sbjct: 181 GMRYYVMAVTPTRLYSFTGTGSLETVFSTYLERAVHFMELPGEIPNRQCYQFRRLIHYSE 240

Query: 241 ------QRRAIHFAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTV 300
                 QRRAIHFAWLSGAGIYHGELNFGSQRS SNGDENFVENKALLDYSKLT+NSGTV
Sbjct: 241 LHFYIKQRRAIHFAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTV 300

Query: 301 KPSSMAVSEFHFLLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---- 360
           KPSSMAVSEFHFLLLIGNKVKVVNRISEQIIEELQFDQTSEAI+RGILGLCSDATA    
Sbjct: 301 KPSSMAVSEFHFLLLIGNKVKVVNRISEQIIEELQFDQTSEAITRGILGLCSDATAGLFY 360

Query: 361 ---------------GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYL 420
                          GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAE ALASRDYL
Sbjct: 361 AYDQNSIFQVSVNDEGRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEDALASRDYL 420

Query: 421 RAASFYAKINYILSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATEL 480
           RAASFYAKINYILSFEEITLKFI A EQDALRTFLLRKLDNLTKDDKCQITMISTWATEL
Sbjct: 421 RAASFYAKINYILSFEEITLKFISAGEQDALRTFLLRKLDNLTKDDKCQITMISTWATEL 480

Query: 481 YLDKINRLLLDDDIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELV 540
           YLDKINRLLLDDD A DGHSTEYQSII+EFRAFLSD KDVLDEVTTMKLLESYGRVEELV
Sbjct: 481 YLDKINRLLLDDDTAFDGHSTEYQSIIQEFRAFLSDSKDVLDEVTTMKLLESYGRVEELV 540

Query: 541 FFAGLKEQYEIVVHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMM 600
           FFAGLKEQYEIVVHHYIQQGEAKKALEVLQKP VPAELQYKFAPELIMLDAYETVESWM+
Sbjct: 541 FFAGLKEQYEIVVHHYIQQGEAKKALEVLQKPGVPAELQYKFAPELIMLDAYETVESWMI 600

Query: 601 TNNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQED 660
           TNNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQED
Sbjct: 601 TNNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQED 660

Query: 661 DSALLRFLQCKFGKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALAL 720
           DSALLRFLQCKFGKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALAL
Sbjct: 661 DSALLRFLQCKFGKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALAL 720

Query: 721 QVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKI 780
           QVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKI
Sbjct: 721 QVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKI 780

Query: 781 EDILPFFPDFALIDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYA 840
           EDILPFFPDFALIDDFKEAIC+SLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYA
Sbjct: 781 EDILPFFPDFALIDDFKEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYA 840

Query: 841 VIDRDEDCGVCKRKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTD 900
           VIDRDEDCGVCKRKILTVGRDLW+ASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTD
Sbjct: 841 VIDRDEDCGVCKRKILTVGRDLWMASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTD 900

Query: 901 EAQAEYILDLQKQITLLGGETRKDSNGNFAEDSISSMT--TADKLRTQLDDAIAGECPFC 918
           EAQAEYILDLQKQITLLGGETRKDSNG+FAEDSISSMT   ADKLRTQLDDAIAGECPFC
Sbjct: 901 EAQAEYILDLQKQITLLGGETRKDSNGSFAEDSISSMTMSPADKLRTQLDDAIAGECPFC 960

BLAST of HG10005339 vs. ExPASy TrEMBL
Match: A0A1S3C0V2 (LOW QUALITY PROTEIN: vacuolar protein sorting-associated protein 18 homolog OS=Cucumis melo OX=3656 GN=LOC103495539 PE=3 SV=1)

HSP 1 Score: 1741.9 bits (4510), Expect = 0.0e+00
Identity = 887/968 (91.63%), Postives = 903/968 (93.29%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           ME GRPAFTVDLLERYAAKGRGVISCMAAGNDVI+LGTSKGWVTRYDFGVGDSIDFDLSV
Sbjct: 1   MEQGRPAFTVDLLERYAAKGRGVISCMAAGNDVIMLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLARLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEK KKEKYVKFLFEL+ELPEAFMDLQMETT++LN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKXKKEKYVKFLFELAELPEAFMDLQMETTNVLN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM++YVMAVTPTRLYSFTG GSLE                              QRRAIH
Sbjct: 181 GMRYYVMAVTPTRLYSFTGTGSLETVFSTYLERAVHFMELPGEIPNSELHFYIKQRRAIH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSGAGIYHGELNFGSQRS SNGDENFVENKALLDYSKLT+NSGTVKPSSMAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTVKPSSMAVSEFHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRISEQIIEELQFDQTSEAI+RGILGLCSDATA                
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAITRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAE ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEDALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEI+LKFI A EQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEISLKFISAGEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           D A DGHSTEYQSII+EFRAFLSD KDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DTAFDGHSTEYQSIIQEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKP V AELQYKFAPELIMLDAYETVESWM+TNNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVLAELQYKFAPELIMLDAYETVESWMITNNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAIC+SLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYAVIDRDEDCGVCK
Sbjct: 781 IDDFKEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDLW+ASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDLWMASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISSMT--TADKLRTQLDDAIAGECPFCGELMIREISLPF 918
           QITLLGGETRKDSNG+FAEDSISSMT   ADKLRTQLDDAIAGECPFCGELMIREISLPF
Sbjct: 901 QITLLGGETRKDSNGSFAEDSISSMTMSPADKLRTQLDDAIAGECPFCGELMIREISLPF 960

BLAST of HG10005339 vs. ExPASy TrEMBL
Match: A0A6J1HZJ9 (vacuolar protein sorting-associated protein 18 homolog OS=Cucurbita maxima OX=3661 GN=LOC111468106 PE=3 SV=1)

HSP 1 Score: 1731.5 bits (4483), Expect = 0.0e+00
Identity = 872/966 (90.27%), Postives = 903/966 (93.48%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           M+PGRPAFTVDLLERYAAKGRGVISCM+AGNDVILLGTSKGWVTRYDFG+GDSID+DLSV
Sbjct: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPGEQSIHR+FVDPGGSHCITTIVG  GADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRIFVDPGGSHCITTIVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFEL+ELPEAFMDLQMETTS+LN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM+FYVMAVTPTRLYSFTGIGSLE                              QRRA+H
Sbjct: 181 GMRFYVMAVTPTRLYSFTGIGSLETVFSTYLERAVHFMELPGDIPNSELHFYIKQRRAMH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSGAGIYHGELNFGSQ SSSNGDENFVENKALLDYSKLT+NSG+VKPSS+AVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRI+EQI+EELQFDQT+EAI RGILGLCSDATA                
Sbjct: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLDMKEYTA+LA+CRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEI LKFI A+EQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEIALKFISANEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DI SDGHST+YQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIVSDGHSTDYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKP VPA+LQYKFAPELIMLDAYETVESWM+T+NLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKG ENGP+FFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYA+IDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRD  +ASSYTSVA MAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISSMTTADKLRTQLDDAIAGECPFCGELMIREISLPFIS 918
           QITLLGGETR++SNG+FAEDSISS+T ADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

BLAST of HG10005339 vs. ExPASy TrEMBL
Match: A0A6J1GKZ1 (vacuolar protein sorting-associated protein 18 homolog OS=Cucurbita moschata OX=3662 GN=LOC111454916 PE=3 SV=1)

HSP 1 Score: 1726.1 bits (4469), Expect = 0.0e+00
Identity = 871/966 (90.17%), Postives = 903/966 (93.48%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           M+PGRPAFTVDLLERYAAKGRGVISCM+AGNDVILLGTSKGWVTRYDFG+GDSID+DLSV
Sbjct: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GRPGEQSIHR+FVDPGGSHCITT+VG  GADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFEL+ELPEAFMDLQMETTS+LN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM+FYVMAVTPTRLYSFTGIGSLE                              QRRA+H
Sbjct: 181 GMRFYVMAVTPTRLYSFTGIGSLETVFSTYLERAVHFMELPGDIPNSELHFYIKQRRAMH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSGAGIYHGELNFGSQ SSSNGDENFVENKALLDYSKLT+NSG+VKPSS+AVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRI+EQI+EELQFDQT+EAI RGILGLCSDATA                
Sbjct: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLDMKEYTA+LA+CRDSLQRDQVYLAQAE ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFI ASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DIASDG+ST+YQSIIKEFRAFLSD KDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKP VPA+LQYKFAPELIMLDAYETVESWM+T+NLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKG ENGP+FFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDI+ALAQRYA+IDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRD  +ASSYTSVA MAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISSMTTADKLRTQLDDAIAGECPFCGELMIREISLPFIS 918
           QITLLGGETR++SNG+FAEDSISS+T ADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

BLAST of HG10005339 vs. TAIR 10
Match: AT1G12470.1 (zinc ion binding )

HSP 1 Score: 1483.4 bits (3839), Expect = 0.0e+00
Identity = 735/967 (76.01%), Postives = 818/967 (84.59%), Query Frame = 0

Query: 1   MEPGRPAFTVDLLERYAAKGRGVISCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60
           M+ GR  F+VDLLERYA K RG+I+CMAAGNDVI+LGTSKGW+ RYDFGVG S D DL+V
Sbjct: 1   MDQGRQVFSVDLLERYATKNRGMITCMAAGNDVIVLGTSKGWIIRYDFGVGSSNDIDLAV 60

Query: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRVLTRLKGLVVNTVAWNR 120
           GR GEQSIH+VFVDPGGSHCI T+ G GGA+TFY HAKW KPRVL+RLKGL+VN VAWNR
Sbjct: 61  GRTGEQSIHKVFVDPGGSHCIATVTGVGGAETFYTHAKWLKPRVLSRLKGLLVNAVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELSELPEAFMDLQMETTSILN 180
           Q ITE STKE+ILGT +GQLFE+AVDEK+K+EKY+KFLFEL ELPEAF  LQMET +I +
Sbjct: 121 QQITEVSTKEIILGTQDGQLFEMAVDEKDKREKYIKFLFELEELPEAFKALQMETANISS 180

Query: 181 GMKFYVMAVTPTRLYSFTGIGSLE------------------------------QRRAIH 240
           GM++YVMAVTPTRLYSFTGIG+LE                              QRRA+H
Sbjct: 181 GMRYYVMAVTPTRLYSFTGIGTLESVFASYKERAVHFMELPGEIPNSELHFFIKQRRAVH 240

Query: 241 FAWLSGAGIYHGELNFGSQRSSSNGDENFVENKALLDYSKLTDNSGTVKPSSMAVSEFHF 300
           FAWLSG GIYHG LNFG+Q S  NGDENFVENKALLDYSKL+D +  VKP SMA+SE+HF
Sbjct: 241 FAWLSGTGIYHGGLNFGAQHSYPNGDENFVENKALLDYSKLSDGTEAVKPGSMALSEYHF 300

Query: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATA---------------- 360
           LLLIGNKVKVVNRISEQIIEELQFD TS+++SRGI+GLCSDA+A                
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDITSDSVSRGIIGLCSDASANVFYAYDQNSIFQVSV 360

Query: 361 ---GRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420
              GRDMWKVYLD+K Y AALANCRD LQRDQVYL QAE A   ++YLRAASFYAKINY+
Sbjct: 361 IDEGRDMWKVYLDLKVYAAALANCRDPLQRDQVYLVQAESAFTDKEYLRAASFYAKINYV 420

Query: 421 LSFEEITLKFIGASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           +SFEE+TLKFI  +E +ALRTFLL KLDNL+KDDKCQITMISTWATELYLDKINRLLL+D
Sbjct: 421 ISFEEVTLKFISINEPEALRTFLLHKLDNLSKDDKCQITMISTWATELYLDKINRLLLED 480

Query: 481 DIASDGHSTEYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           D A +   +EY S+I+EFRAF+SDCKD LDE TT+K+LESYGRVEELV+FA LKEQYEIV
Sbjct: 481 DTAIENRDSEYHSVIQEFRAFMSDCKDELDEATTVKILESYGRVEELVYFANLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPRVPAELQYKFAPELIMLDAYETVESWMMTNNLNPRKLIPA 600
           V HYIQQGEAKKALEVLQK  V  ELQY+FAPELIMLDAYETVESWM   NLNPR+LI A
Sbjct: 541 VLHYIQQGEAKKALEVLQKSSVSVELQYQFAPELIMLDAYETVESWMANKNLNPRRLITA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYS  PHAKNETHEVIKYLE+CVHRLHNEDPG+H+LLLSLYAKQEDD ALLRFLQCKF
Sbjct: 601 MMRYSSGPHAKNETHEVIKYLEFCVHRLHNEDPGIHSLLLSLYAKQEDDGALLRFLQCKF 660

Query: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKG+ENGPEFFYDPKYALRLCLKE+R RACVHIYSMM+MHEEAVALALQ+D ELAMAEAD
Sbjct: 661 GKGRENGPEFFYDPKYALRLCLKERRTRACVHIYSMMSMHEEAVALALQIDPELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLM+AKHV++ EKG KRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMVAKHVVKQEKGAKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDISALAQRYAVIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQI+QLK+EMNDAT GADNIR DISAL QRYAVIDRDE+CGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIEQLKEEMNDATRGADNIRNDISALTQRYAVIDRDEECGVCK 840

Query: 841 RKILTVGRDLWLASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKIL +  D  +A  Y+S   +APFYVFPCGH FHAQCLI HVT C  E QAE+ILDLQK
Sbjct: 841 RKILMMSGDFRMAQGYSSAGPLAPFYVFPCGHSFHAQCLITHVTSCAHEEQAEHILDLQK 900

Query: 901 QITLLGGETRKDSNGNFAEDSISSMTTADKLRTQLDDAIAGECPFCGELMIREISLPFIS 919
           Q+TLLG ETR+D NGN +++ I+S TTADKLR++LDDAIA ECPFCGELMI EI+LPFI 
Sbjct: 901 QLTLLGSETRRDINGNRSDEPITSTTTADKLRSELDDAIASECPFCGELMINEITLPFIK 960