HG10005261 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10005261 vs. NCBI nr
Match: XP_011659040.1 (guanine nucleotide exchange factor SPIKE 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 3254.9 bits (8438), Expect = 0.0e+00
Identity = 1681/1836 (91.56%), Postives = 1694/1836 (92.27%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSM FGQRIPETPVT                                
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR  DSTFKK  NNGSDSHHLSGSS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSHHLSGSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNF +GKE SGNGSFSHEN D+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFPVGKEFSGNGSFSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQSVLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKPADG+LMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MASSHSIAT+YGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS+LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRL+VTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. NCBI nr
Match: XP_011659041.1 (guanine nucleotide exchange factor SPIKE 1 isoform X2 [Cucumis sativus] >KAE8646107.1 hypothetical protein Csa_015554 [Cucumis sativus])

HSP 1 Score: 3248.4 bits (8421), Expect = 0.0e+00
Identity = 1680/1836 (91.50%), Postives = 1693/1836 (92.21%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPK IGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPK-IGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSM FGQRIPETPVT                                
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR  DSTFKK  NNGSDSHHLSGSS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSHHLSGSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNF +GKE SGNGSFSHEN D+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFPVGKEFSGNGSFSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQSVLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKPADG+LMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MASSHSIAT+YGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS+LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRL+VTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. NCBI nr
Match: XP_038888190.1 (guanine nucleotide exchange factor SPIKE 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 3241.8 bits (8404), Expect = 0.0e+00
Identity = 1677/1836 (91.34%), Postives = 1690/1836 (92.05%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETI PVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETISPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSMIFGQRIPETPVT                                
Sbjct: 121  LPAYEPAFDWENERSMIFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TG+SSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGTSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRP DSTFKK  NNGSD HH+SGSS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPVDSTFKKHPNNGSDRHHVSGSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            KL+ S+ KE SGNGS  HENAD+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLSCSVVKEFSGNGSSPHENADTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQ VLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQPVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKPADGMLMGSSSRSPAAVGD PGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDVPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFSLMASSHSIAT+YGKLDCITSIFMSFFSKNQPLAF+ ALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFHNALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. NCBI nr
Match: TYJ96782.1 (guanine nucleotide exchange factor SPIKE 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 3237.6 bits (8393), Expect = 0.0e+00
Identity = 1673/1836 (91.12%), Postives = 1687/1836 (91.88%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSM FGQRIPETP T                                
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR  DSTFKK  NNGSDS HLS SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSQHLSCSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            K NF +GKE SGNGS SHEN D+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQSVLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNA EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNATEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECL+LFEHRKPADG+LMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLVLFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MASSHSIAT+YGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS+LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. NCBI nr
Match: XP_008447100.1 (PREDICTED: guanine nucleotide exchange factor SPIKE 1 isoform X1 [Cucumis melo])

HSP 1 Score: 3235.3 bits (8387), Expect = 0.0e+00
Identity = 1672/1836 (91.07%), Postives = 1687/1836 (91.88%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWP+LNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPYLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSM FGQRIPETP T                                
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR  DSTFKK  NNGSDS HLS SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSQHLSCSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            K NF +GKE SGNGS SHEN D+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQSVLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNA EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNATEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECL+LFEHRKPADG+LMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLVLFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MASSHSIAT+YGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS+LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. ExPASy Swiss-Prot
Match: Q8SAB7 (Guanine nucleotide exchange factor SPIKE 1 OS=Arabidopsis thaliana OX=3702 GN=SPK1 PE=1 SV=1)

HSP 1 Score: 2649.4 bits (6866), Expect = 0.0e+00
Identity = 1376/1818 (75.69%), Postives = 1511/1818 (83.11%), Query Frame = 0

Query: 21   EENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQNQIYEGPDTDIETEMRLTYA 80
            +ENLEQWPHLN+LVQCY T+WVKD NKYGHYE I P SFQ QI+EGPDTD ETE+RL  A
Sbjct: 28   DENLEQWPHLNQLVQCYGTEWVKDVNKYGHYENIRPDSFQTQIFEGPDTDTETEIRLASA 87

Query: 81   RRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSPLPAYEPAFDWENERSMIFGQ 140
            R      T E+DV S SGRP S   DP  S   K  G  PLPAYEPAFDWENER+MIFGQ
Sbjct: 88   RSA----TIEEDVASISGRPFS---DPGSS---KHFGQPPLPAYEPAFDWENERAMIFGQ 147

Query: 141  RIPETPVT---------------------------------------------------- 200
            R PE+P                                                      
Sbjct: 148  RTPESPAASYSSGLKISVRVLSLAFQSGLVEPFFGSIALYNQERKEKLSEDFYFQIQPTE 207

Query: 201  ------------------------------------------------------------ 260
                                                                        
Sbjct: 208  MQDAKLSSENRGVFYLDAPSASVCLLIQLEKTATEEGGVTSSVYSRKEPVHLTEREKQKL 267

Query: 261  ---------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNITGSSSHEGVFEPSTKVT 320
                     +E FAWA+V LFDN+    +  SASPSSPLAP++T SSSH+GV+EP  K+T
Sbjct: 268  QVWSRIMPYRESFAWAVVPLFDNNLTTNTGESASPSSPLAPSMTASSSHDGVYEPIAKIT 327

Query: 321  VDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPVKGVLRLEIEKHQISHAD 380
             DGK GYS GSSVVVEISNLNKVKE Y+E+++QDPK KVHKPVKGVLRLEIEKH+  H D
Sbjct: 328  SDGKQGYSGGSSVVVEISNLNKVKESYSEESIQDPKRKVHKPVKGVLRLEIEKHRNGHGD 387

Query: 381  NENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSSKLNFSIGKEISGNGSFS 440
             E++SE+GS+I+DS+D  DR +D T  K  ++ S     +G SK N    K++S N + S
Sbjct: 388  FEDLSENGSIINDSLDPTDRLSDLTLMKCPSSSSGGPR-NGCSKWNSEDAKDVSRNLTSS 447

Query: 441  HENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSRKRNLFIRIELREDDSDP 500
                D N   +HAFDF    RNEPFL LFHCLYVYP+ V+LSRKRN FIR+ELR+DD+D 
Sbjct: 448  CGTPDLNC--YHAFDFCSTTRNEPFLHLFHCLYVYPVAVTLSRKRNPFIRVELRKDDTDI 507

Query: 501  RRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNVD 560
            R+QPLEA+YP E G SLQKW HTQVAVGAR A YHDEIK+SLPATWTP HHLLFTFF+VD
Sbjct: 508  RKQPLEAIYPREPGVSLQKWVHTQVAVGARAASYHDEIKVSLPATWTPSHHLLFTFFHVD 567

Query: 561  MQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYLQDTNRERLDYLEDGKNI 620
            +Q KLEAP+PV +GYASLPLST+   RS+ISLPVMRELVPHYLQ++ +ERLDYLEDGKNI
Sbjct: 568  LQTKLEAPRPVVVGYASLPLSTYIHSRSDISLPVMRELVPHYLQESTKERLDYLEDGKNI 627

Query: 621  FKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLH 680
            FKLRLRLCSSLYP NER+RDF LEYDRHTL+T PPWGSELL+AINSLK+VDSTALLQFL+
Sbjct: 628  FKLRLRLCSSLYPTNERVRDFCLEYDRHTLQTRPPWGSELLQAINSLKHVDSTALLQFLY 687

Query: 681  PILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDFGGR 740
            PILNMLLHLIGNGGETLQVAAFRAMV+I+TRVQQ S +D +RN FLV YVDY+FDDFGG 
Sbjct: 688  PILNMLLHLIGNGGETLQVAAFRAMVDILTRVQQVSFDDADRNRFLVTYVDYSFDDFGGN 747

Query: 741  QPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELIVKSMALEKTRLFYHSLP 800
            QPPVYPGL+TVWGSLARSKAKGYRVGPVYDDVL+MAWFFLELIVKSMALE+ RL+ H+LP
Sbjct: 748  QPPVYPGLATVWGSLARSKAKGYRVGPVYDDVLSMAWFFLELIVKSMALEQARLYDHNLP 807

Query: 801  LGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEP 860
             GED+PPMQLKE VFRCIMQL+DCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLL IIEP
Sbjct: 808  TGEDVPPMQLKESVFRCIMQLFDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLYIIEP 867

Query: 861  RQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMPGRDPSDRNYLSSVLIQE 920
             Q    VSLY+DKFSGVCQSVLH+CKLTFLQ+I DHDLFVEMPGRDPSDRNYLSS+LIQE
Sbjct: 868  CQVYELVSLYMDKFSGVCQSVLHECKLTFLQIISDHDLFVEMPGRDPSDRNYLSSILIQE 927

Query: 921  LFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVF 980
            LFL+ DHD+LPLRAK ARILV+LLCKHEFDARYQK EDKLYIAQLYFP +GQILDEMPVF
Sbjct: 928  LFLSLDHDELPLRAKGARILVILLCKHEFDARYQKAEDKLYIAQLYFPFVGQILDEMPVF 987

Query: 981  YNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADG 1040
            YNLNA EKREVLI +LQIVRNLDDTSLVKAWQQSIARTRL+FKLMEECLILFEH+K AD 
Sbjct: 988  YNLNATEKREVLIGVLQIVRNLDDTSLVKAWQQSIARTRLYFKLMEECLILFEHKKAADS 1047

Query: 1041 MLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRPQGTPDNGNLWQRVNSQ 1100
            +L G++SR P  V +G GSPKYS+RLSPAINNYLSEASRQE R +GTPDNG LWQRVNSQ
Sbjct: 1048 ILGGNNSRGP--VSEGAGSPKYSERLSPAINNYLSEASRQEVRLEGTPDNGYLWQRVNSQ 1107

Query: 1101 LSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQVLEIT 1160
            L+SP+QPYSLREALAQAQSSRIGASAQALRESLHP+LRQKLELWEEN+SA VSLQVLEIT
Sbjct: 1108 LASPSQPYSLREALAQAQSSRIGASAQALRESLHPILRQKLELWEENVSATVSLQVLEIT 1167

Query: 1161 EKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMAR 1220
            E FS MA+SH+IAT+YGKLDCIT+I  SFFS+NQ LAF+KA FP+FN +FDLHGATLMAR
Sbjct: 1168 ENFSSMAASHNIATDYGKLDCITTILTSFFSRNQSLAFWKAFFPIFNRIFDLHGATLMAR 1227

Query: 1221 ENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR-------TARLRVMLIITLSELM 1280
            ENDRFLKQ+ FHLLRLAV+RNDS+RKRAV GLQILV+       TARLR +L ITLSELM
Sbjct: 1228 ENDRFLKQIAFHLLRLAVYRNDSVRKRAVIGLQILVKSSLYFMQTARLRALLTITLSELM 1287

Query: 1281 SDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNECGLPENALVIIPEASADN 1340
            SDVQVT MK++ TLEESGEA+RL++SL +MADE+KS NLL ECGLP++ L+IIPE   +N
Sbjct: 1288 SDVQVTHMKSDNTLEESGEARRLQQSLSEMADEAKSVNLLRECGLPDDTLLIIPEKFTEN 1347

Query: 1341 RWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGFYKLAMAFAPVPDLHIMWL 1400
            RWSW+E+K+LSDSL+LALDASL HALL SVM+MDRYAAAE FYKL MAFAPVPDLHIMWL
Sbjct: 1348 RWSWAEVKHLSDSLVLALDASLGHALLGSVMAMDRYAAAESFYKLGMAFAPVPDLHIMWL 1407

Query: 1401 LHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRICPMVSSEITS 1460
            LHLCDAHQEMQSWAEAAQCAVAVA V+MQALVARNDGVWS+DHV+ALR+ICPMVS E T+
Sbjct: 1408 LHLCDAHQEMQSWAEAAQCAVAVAGVIMQALVARNDGVWSKDHVSALRKICPMVSGEFTT 1467

Query: 1461 EASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRRAYGQL 1520
            EASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSR+AYGQL
Sbjct: 1468 EASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRKAYGQL 1527

Query: 1521 AKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDI 1580
            AKCHTLLTNIYESIL+QES+PIPF DATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDI
Sbjct: 1528 AKCHTLLTNIYESILDQESNPIPFIDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDI 1587

Query: 1581 MEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQITAVDPVIEDEDLGSRRERI 1640
            MEKLSH+YESRMD +H LHIIPDSRQVKAE+LQ GVCYLQITAVD V+EDEDLGSRRERI
Sbjct: 1588 MEKLSHIYESRMDSNHILHIIPDSRQVKAEDLQAGVCYLQITAVDAVMEDEDLGSRRERI 1647

Query: 1641 ISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKS 1700
             SLSTGSVRARVFDRFLFDTPFTKNG+TQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKS
Sbjct: 1648 FSLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKS 1707

Query: 1701 ESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQSLQRILQGSVAVQVNSGVL 1707
            ESLEFSPVENAIGMIETRT ALRNELEEPRSS+GD LPRLQSLQRILQGSVAVQVNSGVL
Sbjct: 1708 ESLEFSPVENAIGMIETRTTALRNELEEPRSSDGDHLPRLQSLQRILQGSVAVQVNSGVL 1767

BLAST of HG10005261 vs. ExPASy Swiss-Prot
Match: Q8BIK4 (Dedicator of cytokinesis protein 9 OS=Mus musculus OX=10090 GN=Dock9 PE=1 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 1.7e-84
Identity = 367/1486 (24.70%), Postives = 626/1486 (42.13%), Query Frame = 0

Query: 342  EPFLQLFHCLYVYPLTV------SLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGAS 401
            +P+    + LYVYP  +      S ++ RN+ I IE ++ D +   QPL+ +Y    G  
Sbjct: 643  QPYTVYSNHLYVYPKYLKYDSQKSFAKARNIAICIEFKDSDEED-SQPLKCIYGRPGGPV 702

Query: 402  LQKWAHTQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNVDM------QAKLEAPKP 461
              + A   V    +   ++DEIK+ LPA    +HHLLFTFF+V          K +    
Sbjct: 703  FTRSALAAVLHHQQNPEFYDEIKIELPAQLHERHHLLFTFFHVSCDNSTKGSTKKKDAVE 762

Query: 462  VPIGYASLPLSTHAQ-LRSEISLPVMRELVPHYL--QDTNRER-----LDYLEDGKNIFK 521
              +G++ LPL    + L SE  +PV   L   YL  Q+    R     + ++E GK + K
Sbjct: 763  TQVGFSWLPLLKDGRVLTSEQHIPVSANLPSGYLGYQELGMGRHYGPEVKWVEGGKPLLK 822

Query: 522  LRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPI 581
            +   L S++Y  ++ + +FF +Y + T   +   GSEL++ + SL  ++   ++ FL  I
Sbjct: 823  ISTHLVSTVYTQDQHLHNFF-QYCQKTESGAQASGSELVKYLKSLHAMEGHVMIAFLPTI 882

Query: 582  LNMLLHLIGNG-GETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQ 641
            LN L  ++     E + V   R ++++V +  +E  E       L +YV +A+      +
Sbjct: 883  LNQLFRVLTRATQEEVAVNVTRVIIHVVAQCHEEGLES-----HLRSYVKFAYK----AE 942

Query: 642  PPVYPGLSTVWGSLARSKAKGYRVGPVY---DDVLAMAWFFLELIVKSMALEKTRLFYHS 701
            P V     TV   L +S     +    +   + +L  +WFF ++++KSMA          
Sbjct: 943  PYVASEYKTVHEELTKSMTTILKPSADFLTSNKLLKYSWFFFDVLIKSMAQHLIENNKVK 1002

Query: 702  LPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSII 761
            L   +  P           +  + + L+  + ++ +     +K  N SLA F     + +
Sbjct: 1003 LLRNQRFP-----ASYHHAVETVVNMLMPHITQKFRDNPEASKNANHSLAVFIKRCFTFM 1062

Query: 762  EP----RQVSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEM-------PGR------ 821
            +     +Q++ Y+  F+      L + K  FL+++C+H+ ++ +        GR      
Sbjct: 1063 DRGFVFKQINNYISCFAPGDPKTLFEYKFEFLRVVCNHEHYIPLNLPMPFGKGRIQRYQD 1122

Query: 822  --------DPSDRN-YLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKP 881
                    D   RN +L  +L++E+  T   +   +R  A  +L  LL KH FD RY   
Sbjct: 1123 LQLDYSLTDEFCRNHFLVGLLLREVG-TALQEFREVRVIAISMLKNLLIKHSFDDRYNSR 1182

Query: 882  EDKLYIAQLYFPLIGQIL---------DEMPVFYNLNAIEKREVLIV------ILQIVRN 941
              +  IA LY PL G ++         D  P   N  +I K E L V      +     N
Sbjct: 1183 SHQARIATLYLPLFGLLIENVQRINVRDVSPFPVNPGSIVKDEALAVPAGNPLMTPQKGN 1242

Query: 942  LDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPK 1001
              D SL K    +I+     +      +    +      ++   S  S         +  
Sbjct: 1243 TLDHSLHKDLLGAISGIASPYTASTPNINSVRNADSRGSLISTDSGNSLPDRNPEKSNSL 1302

Query: 1002 YSDRLSPAINNYLSEASRQE------------FRPQGTPDNG--NLWQRVNS-------- 1061
               + S  + N +    + +            +  +   D+     W + ++        
Sbjct: 1303 DKQQQSGMLGNSVVRCDKLDQSEIKSLLMCFLYVLKSMSDDALFTYWNKASTAELMDFFT 1362

Query: 1062 -------QLSSPNQPYSLREALAQAQSSRIGASAQALR------ESLHPVLRQKLELWEE 1121
                   Q     + Y  R  +  A+  ++G+   ++        S   V+ Q   L E 
Sbjct: 1363 ISEVCLHQFQYMGKRYIARTGMMHARLQQLGSLDNSVTFNHSYGHSEADVVHQ--SLLEA 1422

Query: 1122 NLSAAVSLQVLEITEKFSLMASSHSIATEYGK---LDCITSIFMSFFSKNQPLAFYKALF 1181
            N++  V L  L+    F+L A  + +  ++G    +  +  +++ F  K+Q     K +F
Sbjct: 1423 NIATEVCLTALDTLSLFTL-AFKNQLLADHGHNPLMKKVFDVYLCFLQKHQSEMALKNVF 1482

Query: 1182 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRT----- 1241
                S+     +       D     + + +L+    +  SIR  A   L  L+R      
Sbjct: 1483 TALRSLIYKFPSAFYEGRAD-MCASLCYEVLKCCNSKLSSIRTEASQLLYFLMRNNFDYT 1542

Query: 1242 -----ARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLL 1301
                  R  + +II++S+L++DV    +   GT        R ++SL          +++
Sbjct: 1543 GKKSFVRTHLQVIISVSQLIADV----VGIGGT--------RFQQSL----------SII 1602

Query: 1302 NECGLPENALVIIPEASADNRWSWSELKYLSD---SLLLALDASLEHALLASVMSMDRYA 1361
            N C    N+  II   S       S++K L+    ++L+A     EH         D   
Sbjct: 1603 NNCA---NSDRIIKHTSFS-----SDVKDLTKRIRTVLMATAQMKEH-------ENDPEM 1662

Query: 1362 AAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDG 1421
              +  Y LA ++A  P+L   WL  +   H +    +EAA C V V A+V + L  +   
Sbjct: 1663 LVDLQYSLAKSYASTPELRKTWLDSMARIHVKNGDLSEAAMCYVHVTALVAEYLTRKG-- 1722

Query: 1422 VWSRDHVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELY 1481
               R   TA R I P     I  EAS  E  G        D  ++ L+       +AE Y
Sbjct: 1723 -MFRQGCTAFRVITP----NIDEEASMMEDVGMQDVHFNEDVLMELLEQCADGLWKAERY 1782

Query: 1482 HFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGE 1541
               A I +L+IP+Y+ RR + +LA  +  L   Y  + E   S       TY+RV F+G+
Sbjct: 1783 ELIADIYKLIIPIYEKRRDFERLAHLYDTLHRAYSKVTEVMHSGRRLL-GTYFRVAFFGQ 1842

Query: 1542 K---------------FGKLDRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIP 1601
                            F   D KEY+Y+EP+   L +I ++L  +Y  +  GS  + +I 
Sbjct: 1843 AAQYQFTDSETDVEGFFEDEDGKEYIYKEPKLTPLSEISQRLLKLYSDKF-GSENVKMIQ 1902

Query: 1602 DSRQVKAEELQPGVCYLQITAVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPF 1661
            DS +V  ++L     Y+Q+T V P  ++++L  RR       T   R     RF+F+ PF
Sbjct: 1903 DSGKVNPKDLDSKFAYIQVTHVTPFFDEKELQERR-------TEFERCHNIRRFMFEMPF 1962

Query: 1662 TKNGRTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAAL 1697
            T+ G+ QGG+E+Q KRRT+L     FP +  R+ V      + +P+E AI  +  + A L
Sbjct: 1963 TQTGKRQGGVEEQCKRRTILTAIHCFPYVKKRIPVMYQHHTDLNPIEVAIDEMSKKVAEL 2022

BLAST of HG10005261 vs. ExPASy Swiss-Prot
Match: Q9BZ29 (Dedicator of cytokinesis protein 9 OS=Homo sapiens OX=9606 GN=DOCK9 PE=1 SV=2)

HSP 1 Score: 315.5 bits (807), Expect = 3.8e-84
Identity = 366/1505 (24.32%), Postives = 635/1505 (42.19%), Query Frame = 0

Query: 342  EPFLQLFHCLYVYPLTV------SLSRKRNLFIRIELREDDSDPRRQPLEAMYPVELGAS 401
            +P+    + LYVYP  +      S ++ RN+ I IE ++ D +   QPL+ +Y    G  
Sbjct: 634  QPYTIYTNHLYVYPKYLKYDSQKSFAKARNIAICIEFKDSDEED-SQPLKCIYGRPGGPV 693

Query: 402  LQKWAHTQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNV--DMQAKLEAPK----P 461
              + A   V    +   ++DEIK+ LP     KHHLL TFF+V  D  +K    K     
Sbjct: 694  FTRSAFAAVLHHHQNPEFYDEIKIELPTQLHEKHHLLLTFFHVSCDNSSKGSTKKRDVVE 753

Query: 462  VPIGYASLPLSTHAQ-LRSEISLPVMRELVPHYL--QDTNRER-----LDYLEDGKNIFK 521
              +GY+ LPL    + + SE  +PV   L   YL  Q+    R     + +++ GK + K
Sbjct: 754  TQVGYSWLPLLKDGRVVTSEQHIPVSANLPSGYLGYQELGMGRHYGPEIKWVDGGKPLLK 813

Query: 522  LRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLHPI 581
            +   L S++Y  ++ + +FF +Y + T   +   G+EL++ + SL  ++   ++ FL  I
Sbjct: 814  ISTHLVSTVYTQDQHLHNFF-QYCQKTESGAQALGNELVKYLKSLHAMEGHVMIAFLPTI 873

Query: 582  LNMLLHLIGNG-GETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDFGGRQ 641
            LN L  ++     E + V   R ++++V +  +E  E       L +YV YA+      +
Sbjct: 874  LNQLFRVLTRATQEEVAVNVTRVIIHVVAQCHEEGLES-----HLRSYVKYAYK----AE 933

Query: 642  PPVYPGLSTVWGSLARSKAKGYRVGPVY---DDVLAMAWFFLELIVKSMALEKTRLFYHS 701
            P V     TV   L +S     +    +   + +L  +WFF ++++KSMA          
Sbjct: 934  PYVASEYKTVHEELTKSMTTILKPSADFLTSNKLLKYSWFFFDVLIKSMAQHLIENSKVK 993

Query: 702  LPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSII 761
            L   +  P           +  + + L+  + ++ +     +K  N SLA F     + +
Sbjct: 994  LLRNQRFP-----ASYHHAVETVVNMLMPHITQKFRDNPEASKNANHSLAVFIKRCFTFM 1053

Query: 762  EP----RQVSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEM-------PGR------ 821
            +     +Q++ Y+  F+      L + K  FL+++C+H+ ++ +        GR      
Sbjct: 1054 DRGFVFKQINNYISCFAPGDPKTLFEYKFEFLRVVCNHEHYIPLNLPMPFGKGRIQRYQD 1113

Query: 822  --------DPSDRN-YLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKP 881
                    D   RN +L  +L++E+  T   +   +R  A  +L  LL KH FD RY   
Sbjct: 1114 LQLDYSLTDEFCRNHFLVGLLLREVG-TALQEFREVRLIAISVLKNLLIKHSFDDRYASR 1173

Query: 882  EDKLYIAQLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTS--------LV 941
              +  IA LY PL G +++      N+  I  R+V    +     + D S        LV
Sbjct: 1174 SHQARIATLYLPLFGLLIE------NVQRINVRDVSPFPVNAGMTVKDESLALPAVNPLV 1233

Query: 942  KAWQQSIARTRLFFKLMEECLIL---FEHRKPADGMLMGSSSR-SPAAVGDGPGSPKYSD 1001
               + S     L   L+     +   +    P    +  + SR S  +   G   P+ + 
Sbjct: 1234 TPQKGSTLDNSLHKDLLGAISGIASPYTTSTPNINSVRNADSRGSLISTDSGNSLPERNS 1293

Query: 1002 RLSPAINNYLSEAS------------RQEFRP---------QGTPDNG--NLWQRVNS-- 1061
              S +++ +   ++            + E +          +   D+     W + ++  
Sbjct: 1294 EKSNSLDKHQQSSTLGNSVVRCDKLDQSEIKSLLMCFLYILKSMSDDALFTYWNKASTSE 1353

Query: 1062 -------------QLSSPNQPYSLREALAQAQSSRIGASAQALR------ESLHPVLRQK 1121
                         Q     + Y  R  +  A+  ++G+   +L        S   VL Q 
Sbjct: 1354 LMDFFTISEVCLHQFQYMGKRYIARTGMMHARLQQLGSLDNSLTFNHSYGHSDADVLHQ- 1413

Query: 1122 LELWEENLSAAVSLQVLEITEKFSLMASSHSIATEYGK---LDCITSIFMSFFSKNQPLA 1181
              L E N++  V L  L+    F+L A  + +  ++G    +  +  +++ F  K+Q   
Sbjct: 1414 -SLLEANIATEVCLTALDTLSLFTL-AFKNQLLADHGHNPLMKKVFDVYLCFLQKHQSET 1473

Query: 1182 FYKALFPVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR 1241
              K +F    S+     +T      D     + + +L+    +  SIR  A   L  L+R
Sbjct: 1474 ALKNVFTALRSLIYKFPSTFYEGRAD-MCAALCYEILKCCNSKLSSIRTEASQLLYFLMR 1533

Query: 1242 T----------ARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADES 1301
                        R  + +II++S+L++DV    +   GT        R ++SL       
Sbjct: 1534 NNFDYTGKKSFVRTHLQVIISVSQLIADV----VGIGGT--------RFQQSL------- 1593

Query: 1302 KSSNLLNECGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMD 1361
               +++N C    + L+     S+D +    +L     ++L+A     EH         D
Sbjct: 1594 ---SIINNCA-NSDRLIKHTSFSSDVK----DLTKRIRTVLMATAQMKEH-------END 1653

Query: 1362 RYAAAEGFYKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVAR 1421
                 +  Y LA ++A  P+L   WL  +   H +    +EAA C V V A+V + L  +
Sbjct: 1654 PEMLVDLQYSLAKSYASTPELRKTWLDSMARIHVKNGDLSEAAMCYVHVTALVAEYLTRK 1713

Query: 1422 NDGVWS-----RDHVTALRR-----------ICPMVSSEITSEASAAEVEGYGASKLTVD 1481
                W        H   LRR              +++  I  EAS  E  G        D
Sbjct: 1714 EAVQWEPPLLPHSHSACLRRSRGGVFRQGCTAFRVITPNIDEEASMMEDVGMQDVHFNED 1773

Query: 1482 SAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQE 1541
              ++ L+       +AE Y   A I +L+IP+Y+ RR + +LA  +  L   Y  + E  
Sbjct: 1774 VLMELLEQCADGLWKAERYELIADIYKLIIPIYEKRRDFERLAHLYDTLHRAYSKVTEVM 1833

Query: 1542 SSPIPFTDATYYRVGFYGEK---------------FGKLDRKEYVYREPRDVRLGDIMEK 1601
             S       TY+RV F+G+                F   D KEY+Y+EP+   L +I ++
Sbjct: 1834 HSGRRLL-GTYFRVAFFGQAAQYQFTDSETDVEGFFEDEDGKEYIYKEPKLTPLSEISQR 1893

Query: 1602 LSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQITAVDPVIEDEDLGSRRERIISL 1661
            L  +Y  +  GS  + +I DS +V  ++L     Y+Q+T V P  ++++L  R+      
Sbjct: 1894 LLKLYSDKF-GSENVKMIQDSGKVNPKDLDSKYAYIQVTHVIPFFDEKELQERK------ 1953

Query: 1662 STGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKSESL 1697
             T   R+    RF+F+ PFT+ G+ QGG+E+Q KRRT+L     FP +  R+ V      
Sbjct: 1954 -TEFERSHNIRRFMFEMPFTQTGKRQGGVEEQCKRRTILTAIHCFPYVKKRIPVMYQHHT 2013

BLAST of HG10005261 vs. ExPASy Swiss-Prot
Match: Q96N67 (Dedicator of cytokinesis protein 7 OS=Homo sapiens OX=9606 GN=DOCK7 PE=1 SV=4)

HSP 1 Score: 312.8 bits (800), Expect = 2.4e-83
Identity = 373/1643 (22.70%), Postives = 637/1643 (38.77%), Query Frame = 0

Query: 351  LYVYPLTVSLSRK----RNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAV 410
            LY+YP +++ + +    RN+ ++++    +      P+  ++     +   K A+T V  
Sbjct: 564  LYIYPQSLNFANRQGSARNITVKVQFMYGEDPSNAMPV--IFGKSSCSEFSKEAYTAVVY 623

Query: 411  GARVACYHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLR 470
              R   +H+EIK+ LPAT T  HHLLFTF++V  Q K   P   P+GY  +P+  + +L+
Sbjct: 624  HNRSPDFHEEIKVKLPATLTDHHHLLFTFYHVSCQQKQNTPLETPVGYTWIPMLQNGRLK 683

Query: 471  S-EISLPVMRELVPHYLQDTNRE----RLDYLEDGKNIFKLRLRLCSSLYPINERIRDFF 530
            + +  LPV  E  P      + E     + ++++ K +F + +   SS++  +  +  FF
Sbjct: 684  TGQFCLPVSLEKPPQAYSVLSPEVPLPGMKWVDNHKGVFNVEVVAVSSIHTQDPYLDKFF 743

Query: 531  L---EYDRHTLRT--------SPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIG 590
                  D H                 +EL  +I++L +     +++FLH +L+ L+ L+ 
Sbjct: 744  ALVNALDEHLFPVRIGDMRIMENNLENELKSSISALNSSQLEPVVRFLHLLLDKLILLVI 803

Query: 591  N----GGETLQV--AAFRAMVNIVTRVQQE---SAEDGERNHFLVNYVDYAFDDFGGRQP 650
                  G+ + +  A+F AM +I+ R+ +    + +   RN  L +Y+ Y F     R P
Sbjct: 804  RPPVIAGQIVNLGQASFEAMASIINRLHKNLEGNHDQHGRNSLLASYIHYVF-----RLP 863

Query: 651  PVYPGLST---------------------------------------------------- 710
              YP  S+                                                    
Sbjct: 864  NTYPNSSSPGPGGLGGSVHYATMARSAVRPASLNLNRSRSLSNSNPDISGTPTSPDDEVR 923

Query: 711  -----------------------VWGSLARSKAKGYRV---------------------- 770
                                    WGS     A+  +                       
Sbjct: 924  SIIGSKGLDRSNSWVNTGGPKAAPWGSNPSPSAESTQAMDRSCNRMSSHTETSSFLQTLT 983

Query: 771  ---------------------GPVYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGED 830
                                 G V +  L  AWFF EL+VKSM         H L   + 
Sbjct: 984  GRLPTKKLFHEELALQWVVCSGSVRESALQQAWFFFELMVKSMV--------HHLYFNDK 1043

Query: 831  IP---PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEP- 890
            +      +  E     I  L   + +++  R +K   + +RLN+SLAFF  DLLS+++  
Sbjct: 1044 LEAPRKSRFPERFMDDIAALVSTIASDIVSRFQKDTEMVERLNTSLAFFLNDLLSVMDRG 1103

Query: 891  ----------RQVSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEM--------PGRD 950
                      +QVS  L  +S    SVL   +L FL++IC H+ +V +        P   
Sbjct: 1104 FVFSLIKSCYKQVSSKL--YSLPNPSVLVSLRLDFLRIICSHEHYVTLNLPCSLLTPPAS 1163

Query: 951  PS---------------------------------DRNYLSSVLIQELFLTWDHDD---L 1010
            PS                                  ++YL+ +++ EL +  D D     
Sbjct: 1164 PSPSVSSATSQSSGFSTNVQDQKIANMFELSVPFRQQHYLAGLVLTELAVILDPDAEGLF 1223

Query: 1011 PLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYN-------- 1070
             L  K   ++  LL  H+ D RY  P+ K  +A LY PLIG I++ +P  Y+        
Sbjct: 1224 GLHKKVINMVHNLLSSHDSDPRYSDPQIKARVAMLYLPLIGIIMETVPQLYDFTETHNQR 1283

Query: 1071 -----------------------------------------------------LNAIEKR 1130
                                                                  +A   R
Sbjct: 1284 GRPICIATDDYESESGSMISQTVAMAIAGTSVPQLTRPGSFLLTSTSGRQHTTFSAESSR 1343

Query: 1131 EVLIVILQIVRNLDDTSLVKAWQQ-SIARTRLFFKLMEECLILFEHRKPADGMLMGSSSR 1190
             +LI +L +++N D+T L K +   S+ +      L+  C+  FE++       M S + 
Sbjct: 1344 SLLICLLWVLKNADETVLQKWFTDLSVLQLNRLLDLLYLCVSCFEYKGKKVFERMNSLTF 1403

Query: 1191 SPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRPQGTPDNGNLWQRVNSQLSSPNQPY 1250
                      S     +L  AI   +        R +G        Q     ++SP +  
Sbjct: 1404 KK--------SKDMRAKLEEAILGSIGARQEMVRRSRG--------QLGTYTIASPPERS 1463

Query: 1251 SLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEE-----NLSAAVSLQVLE----I 1310
                A    ++ R        R++   + + + E+  E     NL+   +L +L+    +
Sbjct: 1464 PSGSAFGSQENLRWRKDMTHWRQNTEKLDKSRAEIEHEALIDGNLATEANLIILDTLEIV 1523

Query: 1311 TEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMA 1370
             +  S+  S  SI      L  +  + +   + NQ   + +  F    ++       L  
Sbjct: 1524 VQTVSVTESKESI------LGGVLKVLLHSMACNQSAVYLQHCFATQRALVSKFPELLFE 1583

Query: 1371 RENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRT--------ARLRVMLIITLSE 1430
             E ++    +   LLR       +IR  A   L +L+R         AR+++ + ++LS 
Sbjct: 1584 EETEQ-CADLCLRLLRHCSSSIGTIRSHASASLYLLMRQNFEIGNNFARVKMQVTMSLSS 1643

Query: 1431 LMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNECGLPENALVIIPEASA 1490
            L+           GT +   E + LR+SL+ +   ++    L E   P+           
Sbjct: 1644 LV-----------GTSQNFNE-EFLRRSLKTILTYAEEDLELRETTFPDQV--------- 1703

Query: 1491 DNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGFYKLAMAFAPVPDLHIM 1550
                           L+  L   L   +       D     +  Y++A  +   PDL + 
Sbjct: 1704 -------------QDLVFNLHMILSDTVKMKEHQEDPEMLIDLMYRIAKGYQTSPDLRLT 1763

Query: 1551 WLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRICPMV--SS 1610
            WL ++   H E  + AEAAQC V  AA+V + L    D  +        + I   V   S
Sbjct: 1764 WLQNMAGKHSERSNHAEAAQCLVHSAALVAEYLSMLEDRKYLPVGCVTFQNISSNVLEES 1823

Query: 1611 EITSEASAAEVEGYGASKLTVDSA-VKYLQLANKLFSQAELYHFCASILELVIPVYKSRR 1670
             ++ +  + + EG  + K   +S  V  L+ A   FS A +Y     + +++IP++++ R
Sbjct: 1824 AVSDDVVSPDEEGICSGKYFTESGLVGLLEQAAASFSMAGMYEAVNEVYKVLIPIHEANR 1883

Query: 1671 AYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYREPRDV 1707
               +L+  H  L   +  I+ Q S+       TY+RVGFYG KFG LD +E+VY+EP   
Sbjct: 1884 DAKKLSTIHGKLQEAFSKIVHQ-STGWERMFGTYFRVGFYGTKFGDLDEQEFVYKEPAIT 1943

BLAST of HG10005261 vs. ExPASy Swiss-Prot
Match: Q8R1A4 (Dedicator of cytokinesis protein 7 OS=Mus musculus OX=10090 GN=Dock7 PE=1 SV=3)

HSP 1 Score: 312.0 bits (798), Expect = 4.2e-83
Identity = 384/1643 (23.37%), Postives = 642/1643 (39.07%), Query Frame = 0

Query: 351  LYVYPLTVSLSRK----RNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAV 410
            LY+YP +++ + +    RN+ ++++    +      P+  ++     +   K A+T V  
Sbjct: 564  LYIYPQSLNFANRQGSARNITVKVQFMYGEDPSNAMPV--IFGKSSCSEFSKEAYTAVVY 623

Query: 411  GARVACYHDEIKLSLPATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLR 470
              R   +H+EIK+ LPAT T  HHLLFTF++V  Q K   P   P+GY  +P+  + +L+
Sbjct: 624  HNRSPDFHEEIKVKLPATLTDHHHLLFTFYHVSCQQKQNTPLETPVGYTWIPMLQNGRLK 683

Query: 471  S-EISLPVMRELVPHYLQDTNRE----RLDYLEDGKNIFKLRLRLCSSLYPINERIRDFF 530
            + +  LPV  E  P      + E     + ++++ K +F + +   SS++  +  +  FF
Sbjct: 684  TGQFCLPVSLEKPPQAYSVLSPEVPLPGMKWVDNHKGVFNVEVVAVSSIHTQDPYLDKFF 743

Query: 531  L---EYDRHTLRT--------SPPWGSELLEAINSLKNVDSTALLQFLHPILNMLLHLIG 590
                  D H                 SEL  +I++L +     +++FLH +L+ L+ L+ 
Sbjct: 744  ALVNALDEHMFPVRIGDMRIMENNLESELKSSISALNSSQLEPVVRFLHLLLDKLILLVV 803

Query: 591  N----GGETLQV--AAFRAMVNIVTRVQQE---SAEDGERNHFLVNYVDYAFDDFGGRQP 650
                  G+ + +  A+F AM +I+ R+ +    + +   RN+ L +Y+ Y F     R P
Sbjct: 804  RPPVIAGQIVNLGQASFEAMASIINRLHKNLEGNHDQHGRNNLLASYIYYVF-----RLP 863

Query: 651  PVYP----------GLSTVWGSLARSKAKGYRV--------------------------- 710
              YP          G S  + ++ARS  +   +                           
Sbjct: 864  NTYPNSPSPGPGGLGGSVHYATMARSAVRPASLNLNRSRSLSNSNPDISGTPTSPDDEVR 923

Query: 711  ------------------------------------------------------------ 770
                                                                        
Sbjct: 924  SIIGSKGLDRSNSWVNTGPKAAPWGSNPSPSAESTQAMDRSCNRMSSHTETSSFLQTLTG 983

Query: 771  --------------------GPVYDDVLAMAWFFLELIVKSMALEKTRLFYHSLPLGE-- 830
                                G V +  L  AWFF EL+VKSM         H L   +  
Sbjct: 984  RLPTKKLFHEELALQWVVCSGSVRESALQQAWFFFELMVKSMV--------HHLYFNDKL 1043

Query: 831  DIP-PMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEP-- 890
            D P   +  E     I  L   +  +V  R +K   + +RLN+SLAFF  DLLS+++   
Sbjct: 1044 DAPRESRFPERFMDDIAALVSTIAGDVVSRFQKDTEMVERLNTSLAFFLNDLLSVMDRGF 1103

Query: 891  ---------RQVSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEM--------PGRDP 950
                     +QVS  L  +S    SVL   +L FL++IC H+ +V +        P   P
Sbjct: 1104 VFSLIKSCYKQVSAKL--YSLPNPSVLVSLRLDFLRIICSHEHYVTLNLPCSLLTPPASP 1163

Query: 951  S---------------------------------DRNYLSSVLIQELFLTWDHDD---LP 1010
            S                                  ++YL+ +++ EL L  D D      
Sbjct: 1164 SPSVSSATSQSSGFSTSVQDQKIANMFELSLPFRQQHYLAGLVLTELALILDPDAEGLFG 1223

Query: 1011 LRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVFYN--------- 1070
            L  K   ++  LL  H+ D RY  P+ K  +A LY PLIG I++ +P  Y+         
Sbjct: 1224 LHKKVINMVHNLLSTHDSDPRYSDPQIKARVAMLYLPLIGIIMETVPQLYDFTESHNQRG 1283

Query: 1071 ----------------------------------------------------LNAIEKRE 1130
                                                                 +A   R 
Sbjct: 1284 RPICIAPDDYDSESGSMISQTVAMAIAGTSVPQLTRPGSFLLTSTSGRQHTTFSAESSRS 1343

Query: 1131 VLIVILQIVRNLDDTSLVKAWQQ-SIARTRLFFKLMEECLILFEHRKPADGMLMGSSSRS 1190
            +LI +L +++N D+T L K +   S+ +      L+  C+  FE++       M S +  
Sbjct: 1344 LLICLLWVLKNADETVLQKWFTDLSVLQLNRLLDLLYLCVSCFEYKGKKVFERMNSLTFK 1403

Query: 1191 PAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRPQGTPDNGNLWQRVNSQLS-SPNQPY 1250
                     S     +L  AI    S  +RQE           + +R   QL  SP+   
Sbjct: 1404 K--------SKDMRAKLEEAILG--SIGARQE-----------MVRRSRGQLERSPS--- 1463

Query: 1251 SLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEE-----NLSAAVSLQVLE----I 1310
                A    ++ R        R++   + + + E+  E     NL+   +L +L+    I
Sbjct: 1464 --GSAFGSQENLRWRKDMTHWRQNSEKLDKSRAEIEHEALIDGNLATEANLIILDTLEII 1523

Query: 1311 TEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMA 1370
             +  S+  S  SI      L  +  + +   + NQ   + +  F    ++       L  
Sbjct: 1524 VQTVSVTESKESI------LGGVLKVLLQSMACNQSAVYLQHCFATQRALVSKFPELLFE 1583

Query: 1371 RENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRT--------ARLRVMLIITLSE 1430
             E ++    +   LLR       +IR  A   L +L+R         AR+++ + ++LS 
Sbjct: 1584 EETEQ-CADLCLRLLRHCSSSISTIRSHASASLYLLMRQNFEIGNNFARVKMQVTMSLSS 1643

Query: 1431 LMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNECGLPENALVIIPEASA 1490
            L+           GT +   E + LR+SL+ +   ++    L E   P+           
Sbjct: 1644 LV-----------GTSQNFNE-EFLRRSLKTILTYAEEDLELRETTFPDQV--------- 1703

Query: 1491 DNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGFYKLAMAFAPVPDLHIM 1550
                           L+  L   L   +       D     +  Y++A  +   PDL + 
Sbjct: 1704 -------------QDLVFNLHMILSDTVKMKEHQEDPEMLIDLMYRIAKGYQTSPDLRLT 1763

Query: 1551 WLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRICPMV--SS 1610
            WL ++   H E  + AEAAQC V  AA+V + L    D  +        + I   V   S
Sbjct: 1764 WLQNMAGKHSERSNHAEAAQCLVHSAALVAEYLSMLEDRKYLPVGCVTFQNISSNVLEES 1823

Query: 1611 EITSEASAAEVEGYGASKLTVDSA-VKYLQLANKLFSQAELYHFCASILELVIPVYKSRR 1670
             ++ +  + + EG  + K   +S  V  L+ A   FS A +Y     + +++IP++++ R
Sbjct: 1824 AVSDDVVSPDEEGICSGKYFTESGLVGLLEQAAASFSMAGMYEAVNEVYKVLIPIHEANR 1883

Query: 1671 AYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYREPRDV 1707
               +L+  H  L   +  I+ Q S+       TY+RVGFYG KFG LD +E+VY+EP   
Sbjct: 1884 DAKKLSTIHGKLQEAFSKIVHQ-STGWERMFGTYFRVGFYGTKFGDLDEQEFVYKEPAIT 1943

BLAST of HG10005261 vs. ExPASy TrEMBL
Match: A0A0A0K3K4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G238980 PE=3 SV=1)

HSP 1 Score: 3254.9 bits (8438), Expect = 0.0e+00
Identity = 1681/1836 (91.56%), Postives = 1694/1836 (92.27%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSM FGQRIPETPVT                                
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPVTHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR  DSTFKK  NNGSDSHHLSGSS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSHHLSGSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            KLNF +GKE SGNGSFSHEN D+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KLNFPVGKEFSGNGSFSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQSVLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECLILFEHRKPADG+LMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLILFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MASSHSIAT+YGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS+LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRL+VTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLVVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. ExPASy TrEMBL
Match: A0A5D3BAB8 (Guanine nucleotide exchange factor SPIKE 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00800 PE=3 SV=1)

HSP 1 Score: 3237.6 bits (8393), Expect = 0.0e+00
Identity = 1673/1836 (91.12%), Postives = 1687/1836 (91.88%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSM FGQRIPETP T                                
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR  DSTFKK  NNGSDS HLS SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSQHLSCSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            K NF +GKE SGNGS SHEN D+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQSVLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNA EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNATEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECL+LFEHRKPADG+LMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLVLFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MASSHSIAT+YGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS+LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. ExPASy TrEMBL
Match: A0A1S3BGL5 (guanine nucleotide exchange factor SPIKE 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489635 PE=3 SV=1)

HSP 1 Score: 3235.3 bits (8387), Expect = 0.0e+00
Identity = 1672/1836 (91.07%), Postives = 1687/1836 (91.88%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWP+LNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPYLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSM FGQRIPETP T                                
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR  DSTFKK  NNGSDS HLS SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSQHLSCSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            K NF +GKE SGNGS SHEN D+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQSVLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNA EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNATEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECL+LFEHRKPADG+LMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLVLFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MASSHSIAT+YGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS+LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. ExPASy TrEMBL
Match: A0A5A7U5I0 (Guanine nucleotide exchange factor SPIKE 1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold511G00690 PE=3 SV=1)

HSP 1 Score: 3231.0 bits (8376), Expect = 0.0e+00
Identity = 1672/1836 (91.07%), Postives = 1686/1836 (91.83%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPK IGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPK-IGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSM FGQRIPETP T                                
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR  DSTFKK  NNGSDS HLS SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSQHLSCSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            K NF +GKE SGNGS SHEN D+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQSVLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNA EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNATEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECL+LFEHRKPADG+LMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLVLFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MASSHSIAT+YGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS+LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. ExPASy TrEMBL
Match: A0A1S3BH88 (guanine nucleotide exchange factor SPIKE 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489635 PE=3 SV=1)

HSP 1 Score: 3228.7 bits (8370), Expect = 0.0e+00
Identity = 1671/1836 (91.01%), Postives = 1686/1836 (91.83%), Query Frame = 0

Query: 1    MHLLHRRDSTPASIKWQNKFEENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60
            MHLLHRRDSTPASIKW NKFEENLEQWP+LNELVQCYSTDWVKDENKYGHYETIGPVSFQ
Sbjct: 1    MHLLHRRDSTPASIKWHNKFEENLEQWPYLNELVQCYSTDWVKDENKYGHYETIGPVSFQ 60

Query: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSP 120
            NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPK IGPSP
Sbjct: 61   NQIYEGPDTDIETEMRLTYARRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPK-IGPSP 120

Query: 121  LPAYEPAFDWENERSMIFGQRIPETPVT-------------------------------- 180
            LPAYEPAFDWENERSM FGQRIPETP T                                
Sbjct: 121  LPAYEPAFDWENERSMTFGQRIPETPATHGLKISVKVLSLSLQAGLVEPFYGTICLYNRE 180

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 181  RREKLSEDFHFRIAPKEMQDPKISFEPRGIFYLEAPSASVCLFIQLEKHATEEGGVTASV 240

Query: 241  --------------------------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNI 300
                                      +E FAWAIVSLFDNSTGAASAGSASPSSPLAP+I
Sbjct: 241  YSRKEPVHLNEREKQKLQVWSQIMPYRESFAWAIVSLFDNSTGAASAGSASPSSPLAPSI 300

Query: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360
            TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV
Sbjct: 301  TGSSSHEGVFEPSTKVTVDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPV 360

Query: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSS 420
            KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDR  DSTFKK  NNGSDS HLS SS
Sbjct: 361  KGVLRLEIEKHQISHADNENMSESGSVISDSVDMVDRLVDSTFKKFPNNGSDSQHLSCSS 420

Query: 421  KLNFSIGKEISGNGSFSHENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480
            K NF +GKE SGNGS SHEN D+NADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR
Sbjct: 421  KSNFPVGKEFSGNGSLSHENVDTNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSR 480

Query: 481  KRNLFIRIELREDDSDPRRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLP 540
            KRNLFIR+ELREDDSDPRRQPLEAMYPVE+GASLQKWAHTQVAVGARVACYHDEIKLSLP
Sbjct: 481  KRNLFIRVELREDDSDPRRQPLEAMYPVEVGASLQKWAHTQVAVGARVACYHDEIKLSLP 540

Query: 541  ATWTPKHHLLFTFFNVDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600
            ATWTPKHHLLFTFFN+DMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL
Sbjct: 541  ATWTPKHHLLFTFFNIDMQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYL 600

Query: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660
            QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA
Sbjct: 601  QDTNRERLDYLEDGKNIFKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEA 660

Query: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720
            INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN
Sbjct: 661  INSLKNVDSTALLQFLHPILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERN 720

Query: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780
            HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI
Sbjct: 721  HFLVNYVDYAFDDFGGRQPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELI 780

Query: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840
            VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL
Sbjct: 781  VKSMALEKTRLFYHSLPLGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRL 840

Query: 841  NSSLAFFCYDLLSIIEPRQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMP 900
            NSSLAFFCYDLLSIIEPRQ    VSLYLDKFSGVCQSVLHDCKLTFLQ+ICDHDLFVEMP
Sbjct: 841  NSSLAFFCYDLLSIIEPRQVFDLVSLYLDKFSGVCQSVLHDCKLTFLQIICDHDLFVEMP 900

Query: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960
            GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA
Sbjct: 901  GRDPSDRNYLSSVLIQELFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIA 960

Query: 961  QLYFPLIGQILDEMPVFYNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020
            QLYFPLIGQILDEMPVFYNLNA EKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK
Sbjct: 961  QLYFPLIGQILDEMPVFYNLNATEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFK 1020

Query: 1021 LMEECLILFEHRKPADGMLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080
            LMEECL+LFEHRKPADG+LMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR
Sbjct: 1021 LMEECLVLFEHRKPADGVLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFR 1080

Query: 1081 PQGTPDNGNLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140
            PQGTPDNG LWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL
Sbjct: 1081 PQGTPDNGYLWQRVNSQLSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLEL 1140

Query: 1141 WEENLSAAVSLQVLEITEKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALF 1200
            WEENLSAAVSLQVLEITEKFS MASSHSIAT+YGKLDCITSIFMSFFSKNQPLAFYKALF
Sbjct: 1141 WEENLSAAVSLQVLEITEKFSSMASSHSIATDYGKLDCITSIFMSFFSKNQPLAFYKALF 1200

Query: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR------ 1260
            PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR      
Sbjct: 1201 PVFNSVFDLHGATLMARENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVRSSFCHF 1260

Query: 1261 --TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNE 1320
              TARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSS+LLNE
Sbjct: 1261 MQTARLRVMLIITLSELMSDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSSLLNE 1320

Query: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380
            CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF
Sbjct: 1321 CGLPENALVIIPEASADNRWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGF 1380

Query: 1381 YKLAMAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440
            YKLA AFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD
Sbjct: 1381 YKLATAFAPVPDLHIMWLLHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRD 1440

Query: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500
            HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS
Sbjct: 1441 HVTALRRICPMVSSEITSEASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCAS 1500

Query: 1501 ILELVIPVYKSRRAYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560
            ILELVIPVYKSRR+YGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL
Sbjct: 1501 ILELVIPVYKSRRSYGQLAKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKL 1560

Query: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620
            DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT
Sbjct: 1561 DRKEYVYREPRDVRLGDIMEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQIT 1620

Query: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680
            AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL
Sbjct: 1621 AVDPVIEDEDLGSRRERIISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVL 1680

Query: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1707
            QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS
Sbjct: 1681 QTEGSFPALVNRLLVTKSESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQS 1740

BLAST of HG10005261 vs. TAIR 10
Match: AT4G16340.1 (guanyl-nucleotide exchange factors;GTPase binding;GTP binding )

HSP 1 Score: 2649.4 bits (6866), Expect = 0.0e+00
Identity = 1376/1818 (75.69%), Postives = 1511/1818 (83.11%), Query Frame = 0

Query: 21   EENLEQWPHLNELVQCYSTDWVKDENKYGHYETIGPVSFQNQIYEGPDTDIETEMRLTYA 80
            +ENLEQWPHLN+LVQCY T+WVKD NKYGHYE I P SFQ QI+EGPDTD ETE+RL  A
Sbjct: 28   DENLEQWPHLNQLVQCYGTEWVKDVNKYGHYENIRPDSFQTQIFEGPDTDTETEIRLASA 87

Query: 81   RRTKPDDTTEDDVPSTSGRPESTTYDPLLSNVPKQIGPSPLPAYEPAFDWENERSMIFGQ 140
            R      T E+DV S SGRP S   DP  S   K  G  PLPAYEPAFDWENER+MIFGQ
Sbjct: 88   RSA----TIEEDVASISGRPFS---DPGSS---KHFGQPPLPAYEPAFDWENERAMIFGQ 147

Query: 141  RIPETPVT---------------------------------------------------- 200
            R PE+P                                                      
Sbjct: 148  RTPESPAASYSSGLKISVRVLSLAFQSGLVEPFFGSIALYNQERKEKLSEDFYFQIQPTE 207

Query: 201  ------------------------------------------------------------ 260
                                                                        
Sbjct: 208  MQDAKLSSENRGVFYLDAPSASVCLLIQLEKTATEEGGVTSSVYSRKEPVHLTEREKQKL 267

Query: 261  ---------QEPFAWAIVSLFDNSTGAASAGSASPSSPLAPNITGSSSHEGVFEPSTKVT 320
                     +E FAWA+V LFDN+    +  SASPSSPLAP++T SSSH+GV+EP  K+T
Sbjct: 268  QVWSRIMPYRESFAWAVVPLFDNNLTTNTGESASPSSPLAPSMTASSSHDGVYEPIAKIT 327

Query: 321  VDGKLGYSSGSSVVVEISNLNKVKEGYTEDALQDPKHKVHKPVKGVLRLEIEKHQISHAD 380
             DGK GYS GSSVVVEISNLNKVKE Y+E+++QDPK KVHKPVKGVLRLEIEKH+  H D
Sbjct: 328  SDGKQGYSGGSSVVVEISNLNKVKESYSEESIQDPKRKVHKPVKGVLRLEIEKHRNGHGD 387

Query: 381  NENMSESGSVISDSVDMVDRPADSTFKKLHNNGSDSHHLSGSSKLNFSIGKEISGNGSFS 440
             E++SE+GS+I+DS+D  DR +D T  K  ++ S     +G SK N    K++S N + S
Sbjct: 388  FEDLSENGSIINDSLDPTDRLSDLTLMKCPSSSSGGPR-NGCSKWNSEDAKDVSRNLTSS 447

Query: 441  HENADSNADDFHAFDFRVMMRNEPFLQLFHCLYVYPLTVSLSRKRNLFIRIELREDDSDP 500
                D N   +HAFDF    RNEPFL LFHCLYVYP+ V+LSRKRN FIR+ELR+DD+D 
Sbjct: 448  CGTPDLNC--YHAFDFCSTTRNEPFLHLFHCLYVYPVAVTLSRKRNPFIRVELRKDDTDI 507

Query: 501  RRQPLEAMYPVELGASLQKWAHTQVAVGARVACYHDEIKLSLPATWTPKHHLLFTFFNVD 560
            R+QPLEA+YP E G SLQKW HTQVAVGAR A YHDEIK+SLPATWTP HHLLFTFF+VD
Sbjct: 508  RKQPLEAIYPREPGVSLQKWVHTQVAVGARAASYHDEIKVSLPATWTPSHHLLFTFFHVD 567

Query: 561  MQAKLEAPKPVPIGYASLPLSTHAQLRSEISLPVMRELVPHYLQDTNRERLDYLEDGKNI 620
            +Q KLEAP+PV +GYASLPLST+   RS+ISLPVMRELVPHYLQ++ +ERLDYLEDGKNI
Sbjct: 568  LQTKLEAPRPVVVGYASLPLSTYIHSRSDISLPVMRELVPHYLQESTKERLDYLEDGKNI 627

Query: 621  FKLRLRLCSSLYPINERIRDFFLEYDRHTLRTSPPWGSELLEAINSLKNVDSTALLQFLH 680
            FKLRLRLCSSLYP NER+RDF LEYDRHTL+T PPWGSELL+AINSLK+VDSTALLQFL+
Sbjct: 628  FKLRLRLCSSLYPTNERVRDFCLEYDRHTLQTRPPWGSELLQAINSLKHVDSTALLQFLY 687

Query: 681  PILNMLLHLIGNGGETLQVAAFRAMVNIVTRVQQESAEDGERNHFLVNYVDYAFDDFGGR 740
            PILNMLLHLIGNGGETLQVAAFRAMV+I+TRVQQ S +D +RN FLV YVDY+FDDFGG 
Sbjct: 688  PILNMLLHLIGNGGETLQVAAFRAMVDILTRVQQVSFDDADRNRFLVTYVDYSFDDFGGN 747

Query: 741  QPPVYPGLSTVWGSLARSKAKGYRVGPVYDDVLAMAWFFLELIVKSMALEKTRLFYHSLP 800
            QPPVYPGL+TVWGSLARSKAKGYRVGPVYDDVL+MAWFFLELIVKSMALE+ RL+ H+LP
Sbjct: 748  QPPVYPGLATVWGSLARSKAKGYRVGPVYDDVLSMAWFFLELIVKSMALEQARLYDHNLP 807

Query: 801  LGEDIPPMQLKEGVFRCIMQLYDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLSIIEP 860
             GED+PPMQLKE VFRCIMQL+DCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLL IIEP
Sbjct: 808  TGEDVPPMQLKESVFRCIMQLFDCLLTEVHERCKKGLSLAKRLNSSLAFFCYDLLYIIEP 867

Query: 861  RQ----VSLYLDKFSGVCQSVLHDCKLTFLQLICDHDLFVEMPGRDPSDRNYLSSVLIQE 920
             Q    VSLY+DKFSGVCQSVLH+CKLTFLQ+I DHDLFVEMPGRDPSDRNYLSS+LIQE
Sbjct: 868  CQVYELVSLYMDKFSGVCQSVLHECKLTFLQIISDHDLFVEMPGRDPSDRNYLSSILIQE 927

Query: 921  LFLTWDHDDLPLRAKAARILVVLLCKHEFDARYQKPEDKLYIAQLYFPLIGQILDEMPVF 980
            LFL+ DHD+LPLRAK ARILV+LLCKHEFDARYQK EDKLYIAQLYFP +GQILDEMPVF
Sbjct: 928  LFLSLDHDELPLRAKGARILVILLCKHEFDARYQKAEDKLYIAQLYFPFVGQILDEMPVF 987

Query: 981  YNLNAIEKREVLIVILQIVRNLDDTSLVKAWQQSIARTRLFFKLMEECLILFEHRKPADG 1040
            YNLNA EKREVLI +LQIVRNLDDTSLVKAWQQSIARTRL+FKLMEECLILFEH+K AD 
Sbjct: 988  YNLNATEKREVLIGVLQIVRNLDDTSLVKAWQQSIARTRLYFKLMEECLILFEHKKAADS 1047

Query: 1041 MLMGSSSRSPAAVGDGPGSPKYSDRLSPAINNYLSEASRQEFRPQGTPDNGNLWQRVNSQ 1100
            +L G++SR P  V +G GSPKYS+RLSPAINNYLSEASRQE R +GTPDNG LWQRVNSQ
Sbjct: 1048 ILGGNNSRGP--VSEGAGSPKYSERLSPAINNYLSEASRQEVRLEGTPDNGYLWQRVNSQ 1107

Query: 1101 LSSPNQPYSLREALAQAQSSRIGASAQALRESLHPVLRQKLELWEENLSAAVSLQVLEIT 1160
            L+SP+QPYSLREALAQAQSSRIGASAQALRESLHP+LRQKLELWEEN+SA VSLQVLEIT
Sbjct: 1108 LASPSQPYSLREALAQAQSSRIGASAQALRESLHPILRQKLELWEENVSATVSLQVLEIT 1167

Query: 1161 EKFSLMASSHSIATEYGKLDCITSIFMSFFSKNQPLAFYKALFPVFNSVFDLHGATLMAR 1220
            E FS MA+SH+IAT+YGKLDCIT+I  SFFS+NQ LAF+KA FP+FN +FDLHGATLMAR
Sbjct: 1168 ENFSSMAASHNIATDYGKLDCITTILTSFFSRNQSLAFWKAFFPIFNRIFDLHGATLMAR 1227

Query: 1221 ENDRFLKQVTFHLLRLAVFRNDSIRKRAVTGLQILVR-------TARLRVMLIITLSELM 1280
            ENDRFLKQ+ FHLLRLAV+RNDS+RKRAV GLQILV+       TARLR +L ITLSELM
Sbjct: 1228 ENDRFLKQIAFHLLRLAVYRNDSVRKRAVIGLQILVKSSLYFMQTARLRALLTITLSELM 1287

Query: 1281 SDVQVTQMKANGTLEESGEAQRLRKSLEDMADESKSSNLLNECGLPENALVIIPEASADN 1340
            SDVQVT MK++ TLEESGEA+RL++SL +MADE+KS NLL ECGLP++ L+IIPE   +N
Sbjct: 1288 SDVQVTHMKSDNTLEESGEARRLQQSLSEMADEAKSVNLLRECGLPDDTLLIIPEKFTEN 1347

Query: 1341 RWSWSELKYLSDSLLLALDASLEHALLASVMSMDRYAAAEGFYKLAMAFAPVPDLHIMWL 1400
            RWSW+E+K+LSDSL+LALDASL HALL SVM+MDRYAAAE FYKL MAFAPVPDLHIMWL
Sbjct: 1348 RWSWAEVKHLSDSLVLALDASLGHALLGSVMAMDRYAAAESFYKLGMAFAPVPDLHIMWL 1407

Query: 1401 LHLCDAHQEMQSWAEAAQCAVAVAAVVMQALVARNDGVWSRDHVTALRRICPMVSSEITS 1460
            LHLCDAHQEMQSWAEAAQCAVAVA V+MQALVARNDGVWS+DHV+ALR+ICPMVS E T+
Sbjct: 1408 LHLCDAHQEMQSWAEAAQCAVAVAGVIMQALVARNDGVWSKDHVSALRKICPMVSGEFTT 1467

Query: 1461 EASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRRAYGQL 1520
            EASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSR+AYGQL
Sbjct: 1468 EASAAEVEGYGASKLTVDSAVKYLQLANKLFSQAELYHFCASILELVIPVYKSRKAYGQL 1527

Query: 1521 AKCHTLLTNIYESILEQESSPIPFTDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDI 1580
            AKCHTLLTNIYESIL+QES+PIPF DATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDI
Sbjct: 1528 AKCHTLLTNIYESILDQESNPIPFIDATYYRVGFYGEKFGKLDRKEYVYREPRDVRLGDI 1587

Query: 1581 MEKLSHVYESRMDGSHTLHIIPDSRQVKAEELQPGVCYLQITAVDPVIEDEDLGSRRERI 1640
            MEKLSH+YESRMD +H LHIIPDSRQVKAE+LQ GVCYLQITAVD V+EDEDLGSRRERI
Sbjct: 1588 MEKLSHIYESRMDSNHILHIIPDSRQVKAEDLQAGVCYLQITAVDAVMEDEDLGSRRERI 1647

Query: 1641 ISLSTGSVRARVFDRFLFDTPFTKNGRTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKS 1700
             SLSTGSVRARVFDRFLFDTPFTKNG+TQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKS
Sbjct: 1648 FSLSTGSVRARVFDRFLFDTPFTKNGKTQGGLEDQWKRRTVLQTEGSFPALVNRLLVTKS 1707

Query: 1701 ESLEFSPVENAIGMIETRTAALRNELEEPRSSEGDQLPRLQSLQRILQGSVAVQVNSGVL 1707
            ESLEFSPVENAIGMIETRT ALRNELEEPRSS+GD LPRLQSLQRILQGSVAVQVNSGVL
Sbjct: 1708 ESLEFSPVENAIGMIETRTTALRNELEEPRSSDGDHLPRLQSLQRILQGSVAVQVNSGVL 1767