HG10005176 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10005176
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionKetol-acid reductoisomerase
LocationChr07: 257220 .. 260582 (-)
RNA-Seq ExpressionHG10005176
SyntenyHG10005176
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCGCCACATATTTCGCTCCCTCTATCTCAGCCTCATCAACCAAAACCCTAAAACAACCTGCTGCTTTCCAGCCTCTCGCTCCTTCCAGCTTAGGCTTCTTGTCCTCCTCCTCCTCTTCATCAGGATCCAACGCTCTCAAGGCTGTTGGTATTGGTGCTGCTAACCCCGTCGGCTCAGCTTTAGGTGCTCAAATGGTGTCTGTTCCTGCCATCAAACCCTTACCCTCACTCGATTTTGACACATCTGTCTTCAAGAAGGAGAAAGTCAATCTCGCTGGCCATGATGAGGTTTTTACTCCCTTTACTTGTAATGGATTTTGGGGGATGTGAGTTGTGAGTGTGAAACTTTTTTTTTTTTTTTTTGGGAAGGAAATTTATTATGCTTCTATTTCCTGGATTTTTTTTTTTCTACCTTCGGATTTCAGTATATTGTCAGTATATTGTCAGAGGAGGCAGAGATCTTTTTCCCTTGCTGCCCGAAGCCTTCAAGGGAATCAAGAAAATCGGTGTCATTGGCTGGGGTTCCCAGGTAATGTATTCTCACACTCTGCAACCAGTCTTATTGTCATGTTTTGCTGGCTCCATATTCTGATGGTGAAGGATTTAGTAAACTGCAGAATTTAACATATACGAACTTATTTATTTATTTATTGAAATCATGAACGTACTCTTGAAAATTGTTTGTTTCACTTTAAAGTTGATTTTTCTGTTATTAGAAGAAAATGATGTGACATTTTAACTAAATTAAGGATTAATTGGAAAATTTACATTTCAACTTTCAACCTACGTGCAAATATTTTATCTTTTCACTTACGAATATGCTTGGTATGAACAAAAAAAAGTTATGGGATAGAATGTAACAAAGTGACCGACTGGAAATTTTTGTTTCTCTAATGATGTTTTGTTGGTGTGGAAACTAGAGCATTGTATTTTTGAGAAACTGATTTGATTTGTGCATTTGAATGTAGAATGTATGTATGGCTCTGGTTCATTAAAAAATTTCACTCAGAAAAGCGCTTTCTCTTTTCAGGAATACTCAAATTTCTCTAACCTTTTAATTTGTTCTTTATCAGGCACCTGCACAAGCTCAGAATTTAAGGGACTCGCTTGCTGAAGCCAAGTCTGATATTGTGGTTAAGGTGATTTTATTTTATTGTACATTACAATTTACTCAGTCTCTTGTACAAGACTCCTCTCTGGAGTGTTTATTTCCTATAGCATTGTAGGTTGTGATGTTTATTGATTAATCACCTATATGAATGCGGCTTTTTCCATGTTGATTTCTGTCGATACAGATTGGCCTGAGGAAGGGTTCCCGCTCTTTTGCGGAAGCTCGTGCAGTTGGTTTTTCTGAAGAGAATGGAACTTTGGGTGATATATATGAAACTATATCTGAAAGTGATCTTGTCATGCTTCTGATTTCTGATTCTGCACAGGTCAGTTGGTGAAGTTATCTCAACTTTGTTACTTCTAGTACTATTAAGCTAGAGTTAAGTGCTTGTGATAGTAGATATATATGTTTGTTTGATTTCCTGTTTTCTTGGTATTCTGAGGATTATCTACCAATTCCTTTTCTGTATGTTCGCCTACATCCATTCTTCTACATATTTTTTTACAATTGTTTATTTCTCAATATCATTGAACTTTGCAGGCTGATAACTATGAGAAAATATTTTCTCACATGAAACCAAACAGCATTCTTGGGCTTTCCCATGGGTTTCTTCTTGGTCATTTGCAGTCATCCGGGCTTGACTTCCCTAAGAATATTAGCGTGATTGCTGTTTGTCCTAAGGGGATGGGTCCATCTGTGAGGAGACTTTATGTTCAAGGAAAAGAAGTCAATGGTGCAGGAATTAATTCTAGTTTTGCAGTACACCAGGTAATATCGCTTTCTTTCTCCACTTTGAGGTGGATATTGATCGATGTACTTGGTCGCTAACTATGTACAGTTTACAGGATGTTGATGGCAGAGCCACAGATGTTGCATTGGGTTGGTCCGTTGCCCTTGGCTCTCCCTTCACATTTGCTACAACTCTAGAGCAAGAATACAGGAGTGACATCTTTGGCGAGCGAGGTAAACTTTCCACTCTACTTCTTGTGTACATTCAAATACACACAAATTGTGGATACTTAACTTCTTTTGCTGTGGTTGATAGGTATTCTACTCGGTGCTGTTCATGGAATTGTGGAGTCCTTGTTCAGAAGGTACACTGAAAATGGAATGTCTGAAGACCTGGCATACAAGAACACTGTTGAAAGCATAACAGGAATTATATCAAAGACCATTTCCACTAAGGTTGTATCAGCTTACTAGATTTAAGTTTCAGTAATTTTCCATGCATGCACTTCATGTTAATGGCCGTCTGTTTTTCTTCCTGTACAGGGAATGTTGGCAGTGTACAATTCCTTCTCTCCTGATGAGAAAAAGGAATTTGAGAGAGCATATAGTGCATCATATTACCCCTGTATGGATATTCTCTATGAATGTTATGAAGATGTTGCCAGTGGAAGTGAGATCCGCAGTGTTGTGCTTGCTGGCCGACGATTTTATGTATGTTGGTACCTTTTTCCCCCTTAACAGCAATAATATTTGGTGTAATCAAGTAGTTTTTAGTGAAAACAAGCTTATTTATCAGTGATATTTATAATTATGGTTGGATAATTGGTGCCTGTAATTCTTCATAACTTGATCTGCCTTTTATTATTGTTATTATTCTTATGTTACAGGAAAAGGAGGGTCTCCCAGCTTTCCCAATGGGGAACATTGACCAGACCCGGATGTGGAAAGTCGGTGAACGTGTCCGACAATCACGTCCTGCAGGTGACCTCGGCCCGTTGTATCCTTTCACTGCTGGTGTATATGTGGCATTGATGATGGCACAGGTATGAAAATTTCCTGTCTAATAACAATTCGCAGACTCTTAAGAACTTTAAAATTTATTCTATTCCATTTTGTCTGCCTGCTTCATAATAAAATTCTACTTTCAGATCGAGATTCTGAGGAAAAAGGGCCACTCTTACTCTGAGATCATCAACGAAAGTGTGATTGAATCAGTCGACTCTTTGAACCCGTTCATGCATGCTCGTGGTGTGTCTTTCATGGTTGACAACTGTTCCACAACCGCACGATTGGGATCTCGGAAGTGGGCTCCTCGCTTTGACTACATTCTCACCCAACAAGCCCTGGTTGCCATTGACAATGGTTCAGCCATTAACCAGGACCTTATTAGCAACTTCCTATCTGACCCAGTTCATGGAGCAATTGAAGTATGCGCCCAGTTGAGACCTACTGTTGACATTTCTGTTCCAGCGGATGCTGATTTCGTTCGCCCAGAACTACGTCAATTGAGCAACTGA

mRNA sequence

ATGGCCGCCGCCACATATTTCGCTCCCTCTATCTCAGCCTCATCAACCAAAACCCTAAAACAACCTGCTGCTTTCCAGCCTCTCGCTCCTTCCAGCTTAGGCTTCTTGTCCTCCTCCTCCTCTTCATCAGGATCCAACGCTCTCAAGGCTGTTGGTATTGGTGCTGCTAACCCCGTCGGCTCAGCTTTAGGTGCTCAAATGGTGTCTGTTCCTGCCATCAAACCCTTACCCTCACTCGATTTTGACACATCTGTCTTCAAGAAGGAGAAAGTCAATCTCGCTGGCCATGATGAGGTTTTTACTCCCTTTACTTGTAATGGATTTTGGGGGATAGGAGGCAGAGATCTTTTTCCCTTGCTGCCCGAAGCCTTCAAGGGAATCAAGAAAATCGGTGTCATTGGCTGGGGTTCCCAGGCACCTGCACAAGCTCAGAATTTAAGGGACTCGCTTGCTGAAGCCAAGTCTGATATTGTGGTTAAGATTGGCCTGAGGAAGGGTTCCCGCTCTTTTGCGGAAGCTCGTGCAGTTGGTTTTTCTGAAGAGAATGGAACTTTGGGTGATATATATGAAACTATATCTGAAAGTGATCTTGTCATGCTTCTGATTTCTGATTCTGCACAGGCTGATAACTATGAGAAAATATTTTCTCACATGAAACCAAACAGCATTCTTGGGCTTTCCCATGGGTTTCTTCTTGGTCATTTGCAGTCATCCGGGCTTGACTTCCCTAAGAATATTAGCGTGATTGCTGTTTGTCCTAAGGGGATGGGTCCATCTGTGAGGAGACTTTATGTTCAAGGAAAAGAAGTCAATGGTGCAGGAATTAATTCTAGTTTTGCAGTACACCAGGATGTTGATGGCAGAGCCACAGATGTTGCATTGGGTTGGTCCGTTGCCCTTGGCTCTCCCTTCACATTTGCTACAACTCTAGAGCAAGAATACAGGAGTGACATCTTTGGCGAGCGAGGTATTCTACTCGGTGCTGTTCATGGAATTGTGGAGTCCTTGTTCAGAAGGTACACTGAAAATGGAATGTCTGAAGACCTGGCATACAAGAACACTGTTGAAAGCATAACAGGAATTATATCAAAGACCATTTCCACTAAGGGAATGTTGGCAGTGTACAATTCCTTCTCTCCTGATGAGAAAAAGGAATTTGAGAGAGCATATAGTGCATCATATTACCCCTGTATGGATATTCTCTATGAATGTTATGAAGATGTTGCCAGTGGAAGTGAGATCCGCAGTGTTGTGCTTGCTGGCCGACGATTTTATGAAAAGGAGGGTCTCCCAGCTTTCCCAATGGGGAACATTGACCAGACCCGGATGTGGAAAGTCGGTGAACGTGTCCGACAATCACGTCCTGCAGGTGACCTCGGCCCGTTGTATCCTTTCACTGCTGGTGTATATGTGGCATTGATGATGGCACAGATCGAGATTCTGAGGAAAAAGGGCCACTCTTACTCTGAGATCATCAACGAAAGTGTGATTGAATCAGTCGACTCTTTGAACCCGTTCATGCATGCTCGTGGTGTGTCTTTCATGGTTGACAACTGTTCCACAACCGCACGATTGGGATCTCGGAAGTGGGCTCCTCGCTTTGACTACATTCTCACCCAACAAGCCCTGGTTGCCATTGACAATGGTTCAGCCATTAACCAGGACCTTATTAGCAACTTCCTATCTGACCCAGTTCATGGAGCAATTGAAGTATGCGCCCAGTTGAGACCTACTGTTGACATTTCTGTTCCAGCGGATGCTGATTTCGTTCGCCCAGAACTACGTCAATTGAGCAACTGA

Coding sequence (CDS)

ATGGCCGCCGCCACATATTTCGCTCCCTCTATCTCAGCCTCATCAACCAAAACCCTAAAACAACCTGCTGCTTTCCAGCCTCTCGCTCCTTCCAGCTTAGGCTTCTTGTCCTCCTCCTCCTCTTCATCAGGATCCAACGCTCTCAAGGCTGTTGGTATTGGTGCTGCTAACCCCGTCGGCTCAGCTTTAGGTGCTCAAATGGTGTCTGTTCCTGCCATCAAACCCTTACCCTCACTCGATTTTGACACATCTGTCTTCAAGAAGGAGAAAGTCAATCTCGCTGGCCATGATGAGGTTTTTACTCCCTTTACTTGTAATGGATTTTGGGGGATAGGAGGCAGAGATCTTTTTCCCTTGCTGCCCGAAGCCTTCAAGGGAATCAAGAAAATCGGTGTCATTGGCTGGGGTTCCCAGGCACCTGCACAAGCTCAGAATTTAAGGGACTCGCTTGCTGAAGCCAAGTCTGATATTGTGGTTAAGATTGGCCTGAGGAAGGGTTCCCGCTCTTTTGCGGAAGCTCGTGCAGTTGGTTTTTCTGAAGAGAATGGAACTTTGGGTGATATATATGAAACTATATCTGAAAGTGATCTTGTCATGCTTCTGATTTCTGATTCTGCACAGGCTGATAACTATGAGAAAATATTTTCTCACATGAAACCAAACAGCATTCTTGGGCTTTCCCATGGGTTTCTTCTTGGTCATTTGCAGTCATCCGGGCTTGACTTCCCTAAGAATATTAGCGTGATTGCTGTTTGTCCTAAGGGGATGGGTCCATCTGTGAGGAGACTTTATGTTCAAGGAAAAGAAGTCAATGGTGCAGGAATTAATTCTAGTTTTGCAGTACACCAGGATGTTGATGGCAGAGCCACAGATGTTGCATTGGGTTGGTCCGTTGCCCTTGGCTCTCCCTTCACATTTGCTACAACTCTAGAGCAAGAATACAGGAGTGACATCTTTGGCGAGCGAGGTATTCTACTCGGTGCTGTTCATGGAATTGTGGAGTCCTTGTTCAGAAGGTACACTGAAAATGGAATGTCTGAAGACCTGGCATACAAGAACACTGTTGAAAGCATAACAGGAATTATATCAAAGACCATTTCCACTAAGGGAATGTTGGCAGTGTACAATTCCTTCTCTCCTGATGAGAAAAAGGAATTTGAGAGAGCATATAGTGCATCATATTACCCCTGTATGGATATTCTCTATGAATGTTATGAAGATGTTGCCAGTGGAAGTGAGATCCGCAGTGTTGTGCTTGCTGGCCGACGATTTTATGAAAAGGAGGGTCTCCCAGCTTTCCCAATGGGGAACATTGACCAGACCCGGATGTGGAAAGTCGGTGAACGTGTCCGACAATCACGTCCTGCAGGTGACCTCGGCCCGTTGTATCCTTTCACTGCTGGTGTATATGTGGCATTGATGATGGCACAGATCGAGATTCTGAGGAAAAAGGGCCACTCTTACTCTGAGATCATCAACGAAAGTGTGATTGAATCAGTCGACTCTTTGAACCCGTTCATGCATGCTCGTGGTGTGTCTTTCATGGTTGACAACTGTTCCACAACCGCACGATTGGGATCTCGGAAGTGGGCTCCTCGCTTTGACTACATTCTCACCCAACAAGCCCTGGTTGCCATTGACAATGGTTCAGCCATTAACCAGGACCTTATTAGCAACTTCCTATCTGACCCAGTTCATGGAGCAATTGAAGTATGCGCCCAGTTGAGACCTACTGTTGACATTTCTGTTCCAGCGGATGCTGATTTCGTTCGCCCAGAACTACGTCAATTGAGCAACTGA

Protein sequence

MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVGSALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLLPEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITGIISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN
Homology
BLAST of HG10005176 vs. NCBI nr
Match: XP_038888114.1 (ketol-acid reductoisomerase, chloroplastic [Benincasa hispida])

HSP 1 Score: 1107.0 bits (2862), Expect = 0.0e+00
Identity = 571/599 (95.33%), Postives = 576/599 (96.16%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAAATYFAPSIS SSTKTLKQ AA QPL PSSLGFL SSSSSSGSNALKAVG+G AN VG
Sbjct: 1   MAAATYFAPSISVSSTKTLKQAAALQPLTPSSLGFL-SSSSSSGSNALKAVGMGGANGVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA+MVSVPAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLL
Sbjct: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE
Sbjct: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GL
Sbjct: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL
Sbjct: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVA+GSEIRSVVLA
Sbjct: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVATGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI
Sbjct: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ +N
Sbjct: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSNN 589

BLAST of HG10005176 vs. NCBI nr
Match: XP_008455269.1 (PREDICTED: ketol-acid reductoisomerase, chloroplastic [Cucumis melo] >KAA0031534.1 ketol-acid reductoisomerase [Cucumis melo var. makuwa] >TYK06987.1 ketol-acid reductoisomerase [Cucumis melo var. makuwa])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 567/599 (94.66%), Postives = 574/599 (95.83%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAAAT+FAPSISASSTKTLKQ AA QPL+PSSLGFL  SSSSSG  ALKAVGI  AN VG
Sbjct: 1   MAAATHFAPSISASSTKTLKQAAALQPLSPSSLGFL--SSSSSGLYALKAVGICGANGVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA+MVSVPAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLL
Sbjct: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE
Sbjct: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GL
Sbjct: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWS+AL
Sbjct: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+E+LAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           IIS+TISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA
Sbjct: 361 IISRTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI
Sbjct: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ SN
Sbjct: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 588

BLAST of HG10005176 vs. NCBI nr
Match: XP_022952496.1 (ketol-acid reductoisomerase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 561/599 (93.66%), Postives = 572/599 (95.49%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAAATYF+PSISAS +KTLKQ A+  PL PSSLGFLSSSS+SSGS ALKAVG+G  N VG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGSFALKAVGMGGGNAVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA+MVS PAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLL
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           PEAFKGIK+IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE
Sbjct: 121 PEAFKGIKQIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GL
Sbjct: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKN SVIAVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWSVAL
Sbjct: 241 DFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           IISKTISTKGMLAVYNSFSPDEKKEFERAYSAS+YPCMDILYECYEDVASGSEIRSVVLA
Sbjct: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI
Sbjct: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVAIDNGSA+NQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ SN
Sbjct: 541 QALVAIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of HG10005176 vs. NCBI nr
Match: XP_022969037.1 (ketol-acid reductoisomerase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 561/599 (93.66%), Postives = 571/599 (95.33%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAAATYF+PSISAS +KTLKQ A+  PL PSSLGFLSSSS+SSG  ALKAVG+G AN VG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA+MVS PAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLL
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSE
Sbjct: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GL
Sbjct: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKN SVIAVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWSVAL
Sbjct: 241 DFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           IISKTISTKGMLAVYNSFSPDEKKEFERAYSAS+YPCMDILYECYEDVASGSEIRSVVLA
Sbjct: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI
Sbjct: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVAIDNGSA+NQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ SN
Sbjct: 541 QALVAIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of HG10005176 vs. NCBI nr
Match: XP_023554080.1 (ketol-acid reductoisomerase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 560/599 (93.49%), Postives = 572/599 (95.49%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAAATYF+PSISAS +KTLKQ A+  PL PSSLGFLSSSS+SSGS ALKAVG+G  N VG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGSFALKAVGMGGGNAVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA+MVS PAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLL
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           PEAFKGIK+IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE
Sbjct: 121 PEAFKGIKQIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GL
Sbjct: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKN SVIAVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWSVAL
Sbjct: 241 DFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           IISKTISTKGMLAVYNSFSPDEKKEFERAYSAS+YPCMDILYECYEDVA+GSEIRSVVLA
Sbjct: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVATGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI
Sbjct: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVAIDNGSA+NQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ SN
Sbjct: 541 QALVAIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of HG10005176 vs. ExPASy Swiss-Prot
Match: Q05758 (Ketol-acid reductoisomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g58610 PE=1 SV=2)

HSP 1 Score: 963.8 bits (2490), Expect = 9.3e-280
Identity = 504/604 (83.44%), Postives = 539/604 (89.24%), Query Frame = 0

Query: 1   MAAAT-YFAPSIS----ASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGA 60
           MAAAT   APS+S    +SS+KTL    A + LA  ++GFLSSSS S  S      G GA
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTLWSSKA-RTLALPNIGFLSSSSKSLRSLTATVAGNGA 60

Query: 61  ANPVGSALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRD 120
               GS+L A+MVS  A+K   SLDF+TSVFKKEKV+LAG++E             GGRD
Sbjct: 61  ---TGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVR---------GGRD 120

Query: 121 LFPLLPEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA 180
           LF  LP+AFKGIK+IGVIGWGSQ PAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EARA
Sbjct: 121 LFKHLPDAFKGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARA 180

Query: 181 VGFSEENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHL 240
            GF+EE+GTLGDI+ETI+ SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHL
Sbjct: 181 AGFTEESGTLGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHL 240

Query: 241 QSSGLDFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALG 300
           QSSGLDFPKNISV+AVCPKGMGPSVRRLYVQGKE+NGAGIN+SFAVHQDVDGRA DVALG
Sbjct: 241 QSSGLDFPKNISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALG 300

Query: 301 WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV 360
           WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV
Sbjct: 301 WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV 360

Query: 361 ESITGIISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIR 420
           E ITG IS+TIST+GMLAVYNS S + KK+FE AYSAS+YPCM+ILYECYEDV SGSEIR
Sbjct: 361 ECITGTISRTISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIR 420

Query: 421 SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMM 480
           SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVR+SRPAGDLGPLYPFTAGVYVALMM
Sbjct: 421 SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMM 480

Query: 481 AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD 540
           AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD
Sbjct: 481 AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD 540

Query: 541 YILTQQALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELR 600
           YILTQQALVA+D+G+AIN+DLISNF SDPVHGAIEVCAQLRPTVDISVPADADFVRPELR
Sbjct: 541 YILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELR 591

BLAST of HG10005176 vs. ExPASy Swiss-Prot
Match: O82043 (Ketol-acid reductoisomerase, chloroplastic OS=Pisum sativum OX=3888 GN=PGAAIR PE=2 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 1.2e-274
Identity = 487/599 (81.30%), Postives = 526/599 (87.81%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAA T    +  ++S+KTL +P A    AP++L F  S  S     A +++       VG
Sbjct: 1   MAAVTSSCSTAISASSKTLAKPVA-ASFAPTNLSF--SKLSPQSIRARRSI------TVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA  VS P      SLDF+TSVFKKE+VNLAGH+E             GGRDLF LL
Sbjct: 61  SALGATKVSAPPATHPVSLDFETSVFKKERVNLAGHEEYIVR---------GGRDLFHLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           P+AFKGIK+IGVIGWGSQ PAQAQNLRDSL EAKSDIVVK+GLRKGS SF EAR  GFSE
Sbjct: 121 PDAFKGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKVGLRKGSSSFNEAREAGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           E GTLGDI+ETIS SDLV+LLISDSAQADNYEKIFSH+KPNSILGLSHGFLLGHLQS GL
Sbjct: 181 EKGTLGDIWETISGSDLVLLLISDSAQADNYEKIFSHLKPNSILGLSHGFLLGHLQSIGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKN SVIAVCPKGMGPSVRRLYVQGKE+NGAGINSSF VHQDVDGRAT+VALGWSVAL
Sbjct: 241 DFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFGVHQDVDGRATNVALGWSVAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           +ISKTIST+GMLAVYN+ S D KKEFE+AYSAS+YPCM+ILYECYEDVASGSEIRSVVLA
Sbjct: 361 VISKTISTQGMLAVYNALSEDGKKEFEKAYSASFYPCMEILYECYEDVASGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMG IDQTRMWKVGERVR +RPAGDLGPLYPFTAGV+VA+MMAQIE+
Sbjct: 421 GRRFYEKEGLPAFPMGKIDQTRMWKVGERVRSTRPAGDLGPLYPFTAGVFVAMMMAQIEV 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVA+D+G+ INQDLISNF+SDPVHGAI+VCA+LRPT+DISVPA ADFVRPELRQ SN
Sbjct: 541 QALVAVDSGAPINQDLISNFVSDPVHGAIQVCAELRPTLDISVPAAADFVRPELRQCSN 581

BLAST of HG10005176 vs. ExPASy Swiss-Prot
Match: Q01292 (Ketol-acid reductoisomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=AHRI PE=1 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 1.2e-274
Identity = 494/600 (82.33%), Postives = 527/600 (87.83%), Query Frame = 0

Query: 3   AATYFAPSISASSTKTLKQPAAFQPLAPS--SLGFLSSSSSSSGSNALKAVGI--GAANP 62
           AAT F+ S S+SST      A  Q   PS  +LGFL SSS+     +LKA  +    AN 
Sbjct: 5   AATTFSLS-SSSSTSAAASKALKQSPKPSALNLGFLGSSSTIKACRSLKAARVLPSGANG 64

Query: 63  VGSALGAQMVSVPAIK--PLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDL 122
            GSAL AQMVS P+I      + DFD+SVFKKEKV L+GHDE             GGR+L
Sbjct: 65  GGSALSAQMVSAPSINTPSATTFDFDSSVFKKEKVTLSGHDEYIVR---------GGRNL 124

Query: 123 FPLLPEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAV 182
           FPLLP+AFKGIK+IGVIGWGSQAPAQAQNL+DSL EAKSD+VVKIGLRKGS SFAEARA 
Sbjct: 125 FPLLPDAFKGIKQIGVIGWGSQAPAQAQNLKDSLTEAKSDVVVKIGLRKGSNSFAEARAA 184

Query: 183 GFSEENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQ 242
           GFSEENGTLGD++ETIS SDLV+LLISDSAQADNYEK+FSHMKPNSILGLSHGFLLGHLQ
Sbjct: 185 GFSEENGTLGDMWETISGSDLVLLLISDSAQADNYEKVFSHMKPNSILGLSHGFLLGHLQ 244

Query: 243 SSGLDFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGW 302
           S G DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGW
Sbjct: 245 SLGQDFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGW 304

Query: 303 SVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVE 362
           S+ALGSPFTFATTLEQEY+SDIFGERGILLGAVHGIVE LFRRYTE+GMSEDLAYKNTVE
Sbjct: 305 SIALGSPFTFATTLEQEYKSDIFGERGILLGAVHGIVECLFRRYTESGMSEDLAYKNTVE 364

Query: 363 SITGIISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRS 422
            ITG+ISKTISTKGMLA+YNS S + KK+F+ AYSASYYP MDILYECYEDVASGSEIRS
Sbjct: 365 CITGVISKTISTKGMLALYNSLSEEGKKDFQAAYSASYYPSMDILYECYEDVASGSEIRS 424

Query: 423 VVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMA 482
           VVLAGRRFYEKEGLPAFPMG IDQTRMWKVGE+VR  RPAGDLGPLYPFTAGVYVALMMA
Sbjct: 425 VVLAGRRFYEKEGLPAFPMGKIDQTRMWKVGEKVRSVRPAGDLGPLYPFTAGVYVALMMA 484

Query: 483 QIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDY 542
           QIEILRKKGHSYSEIINESVIE+VDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDY
Sbjct: 485 QIEILRKKGHSYSEIINESVIEAVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDY 544

Query: 543 ILTQQALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ 597
           IL+QQALVA+DNG+ INQDLISNFLSDPVH AI VCAQLRP+VDISV ADADFVRPELRQ
Sbjct: 545 ILSQQALVAVDNGAPINQDLISNFLSDPVHEAIGVCAQLRPSVDISVTADADFVRPELRQ 594

BLAST of HG10005176 vs. ExPASy Swiss-Prot
Match: Q65XK0 (Ketol-acid reductoisomerase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0573700 PE=1 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 6.2e-268
Identity = 476/599 (79.47%), Postives = 520/599 (86.81%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAA+T    +++ S  KTL   AA  P AP++   +S   S +    L A          
Sbjct: 1   MAAST----TLALSHPKTLAAAAAAAPKAPTAPAAVSFPVSHAACAPLAA--------RR 60

Query: 61  SALGAQMVSVPAI-KPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPL 120
            A+ A + + PA+   +PSLDFDTSVF KEKV+LAGH+E             GGR+LFPL
Sbjct: 61  RAVTAMVAAPPAVGAAMPSLDFDTSVFNKEKVSLAGHEEYIVR---------GGRNLFPL 120

Query: 121 LPEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFS 180
           LPEAFKGIK+IGVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGS+SF EARA GF+
Sbjct: 121 LPEAFKGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSKSFDEARAAGFT 180

Query: 181 EENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSG 240
           EE+GTLGDI+ET+S SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS+G
Sbjct: 181 EESGTLGDIWETVSGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSAG 240

Query: 241 LDFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVA 300
           LDFPKNISVIAVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWSVA
Sbjct: 241 LDFPKNISVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVA 300

Query: 301 LGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESIT 360
           LGSPFTFATTLEQEY+SDIFGERGILLGAVHGIVE+LFRRYTE GM E++AYKNTVE IT
Sbjct: 301 LGSPFTFATTLEQEYKSDIFGERGILLGAVHGIVEALFRRYTEQGMDEEMAYKNTVEGIT 360

Query: 361 GIISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVL 420
           GIISKTIS KGML VYNS + + KKEF +AYSAS+YPCMDILYECYEDVASGSEIRSVVL
Sbjct: 361 GIISKTISKKGMLEVYNSLTEEGKKEFNKAYSASFYPCMDILYECYEDVASGSEIRSVVL 420

Query: 421 AGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIE 480
           AGRRFYEKEGLPAFPMGNIDQTRMWKVGE+VR +RP  DLGPL+PFTAGVYVALMMAQIE
Sbjct: 421 AGRRFYEKEGLPAFPMGNIDQTRMWKVGEKVRSTRPENDLGPLHPFTAGVYVALMMAQIE 480

Query: 481 ILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILT 540
           +LRKKGHSYSEIINESVIESVDSLNPFMHARGV+FMVDNCSTTARLGSRKWAPRFDYILT
Sbjct: 481 VLRKKGHSYSEIINESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAPRFDYILT 540

Query: 541 QQALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLS 599
           QQA V +D  + INQDLISNF+SDPVHGAIEVCA+LRPTVDISVPA+ADFVRPELRQ S
Sbjct: 541 QQAFVTVDKDAPINQDLISNFMSDPVHGAIEVCAELRPTVDISVPANADFVRPELRQSS 578

BLAST of HG10005176 vs. ExPASy Swiss-Prot
Match: A8ZTR0 (Ketol-acid reductoisomerase (NAD(+)) OS=Desulfococcus oleovorans (strain DSM 6200 / JCM 39069 / Hxd3) OX=96561 GN=Dole_2039 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 2.2e-39
Identity = 110/329 (33.43%), Postives = 172/329 (52.28%), Query Frame = 0

Query: 130 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 189
           I V+G+G Q PAQA NL+D+  E      V IG  +G   + +A A GF     TL  I 
Sbjct: 33  ITVLGYGVQGPAQALNLKDNGFE------VIIGQLEGDAYWEKAIADGF-VPGKTLFPIE 92

Query: 190 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPKNISVI 249
           E   +  ++ +L+SD+ Q   + K+   +K    L  SHGF + +   +G+  PKN+ VI
Sbjct: 93  EAAKKGTIIKMLLSDAGQVAVWPKVKKCLKKGDALYFSHGFGIVYKDQTGIVPPKNVDVI 152

Query: 250 AVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 309
            V PKG G +VRR +      +G+GINSS+AV QD  GRA +  +   +A+GS + F TT
Sbjct: 153 LVAPKGSGTNVRRNF-----KDGSGINSSYAVFQDATGRAEERTIALGIAIGSGYLFPTT 212

Query: 310 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITGIISKTISTK 369
            E+E  SD+ GERG+L+G + G +E+ +    ++G S   A+  TVE +T  + + ++  
Sbjct: 213 FEKEVFSDLTGERGVLMGCLAGTMEAQYNVLRKHGHSPSEAFNETVEELTQSLIRLVAEN 272

Query: 370 GMLAVYNSFSPDEKK---EFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYE 429
           GM  ++ + S   ++   ++   +  +  P  D L   Y  V +G+E R V+        
Sbjct: 273 GMDWMFANCSTTAQRGALDWAPKFRDAVAPVFDSL---YRRVKNGAETRRVLKVNSAPNY 332

Query: 430 KEGLPAFPMGNIDQTRMWKVGERVRQSRP 456
            E L    +  I  + MW+ G  VR  RP
Sbjct: 333 LEKLRK-ELDTIKNSEMWQAGAAVRALRP 345

BLAST of HG10005176 vs. ExPASy TrEMBL
Match: A0A5D3C563 (Ketol-acid reductoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003030 PE=3 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 567/599 (94.66%), Postives = 574/599 (95.83%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAAAT+FAPSISASSTKTLKQ AA QPL+PSSLGFL  SSSSSG  ALKAVGI  AN VG
Sbjct: 1   MAAATHFAPSISASSTKTLKQAAALQPLSPSSLGFL--SSSSSGLYALKAVGICGANGVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA+MVSVPAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLL
Sbjct: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE
Sbjct: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GL
Sbjct: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWS+AL
Sbjct: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+E+LAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           IIS+TISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA
Sbjct: 361 IISRTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI
Sbjct: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ SN
Sbjct: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 588

BLAST of HG10005176 vs. ExPASy TrEMBL
Match: A0A1S3C039 (Ketol-acid reductoisomerase OS=Cucumis melo OX=3656 GN=LOC103495472 PE=3 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 567/599 (94.66%), Postives = 574/599 (95.83%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAAAT+FAPSISASSTKTLKQ AA QPL+PSSLGFL  SSSSSG  ALKAVGI  AN VG
Sbjct: 1   MAAATHFAPSISASSTKTLKQAAALQPLSPSSLGFL--SSSSSGLYALKAVGICGANGVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA+MVSVPAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLL
Sbjct: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE
Sbjct: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GL
Sbjct: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWS+AL
Sbjct: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+E+LAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           IIS+TISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA
Sbjct: 361 IISRTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI
Sbjct: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ SN
Sbjct: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 588

BLAST of HG10005176 vs. ExPASy TrEMBL
Match: A0A6J1GKD7 (Ketol-acid reductoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111455167 PE=3 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 561/599 (93.66%), Postives = 572/599 (95.49%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAAATYF+PSISAS +KTLKQ A+  PL PSSLGFLSSSS+SSGS ALKAVG+G  N VG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGSFALKAVGMGGGNAVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA+MVS PAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLL
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           PEAFKGIK+IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE
Sbjct: 121 PEAFKGIKQIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GL
Sbjct: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKN SVIAVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWSVAL
Sbjct: 241 DFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           IISKTISTKGMLAVYNSFSPDEKKEFERAYSAS+YPCMDILYECYEDVASGSEIRSVVLA
Sbjct: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI
Sbjct: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVAIDNGSA+NQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ SN
Sbjct: 541 QALVAIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of HG10005176 vs. ExPASy TrEMBL
Match: A0A6J1HV87 (Ketol-acid reductoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111468154 PE=3 SV=1)

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 561/599 (93.66%), Postives = 571/599 (95.33%), Query Frame = 0

Query: 1   MAAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVG 60
           MAAATYF+PSISAS +KTLKQ A+  PL PSSLGFLSSSS+SSG  ALKAVG+G AN VG
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60

Query: 61  SALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLL 120
           SALGA+MVS PAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLL
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLL 120

Query: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSE 180
           PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR VGFSE
Sbjct: 121 PEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSE 180

Query: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGL 240
           ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GL
Sbjct: 181 ENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGL 240

Query: 241 DFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVAL 300
           DFPKN SVIAVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWSVAL
Sbjct: 241 DFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVAL 300

Query: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITG 360
           GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+EDLAYKNTVESITG
Sbjct: 301 GSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITG 360

Query: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLA 420
           IISKTISTKGMLAVYNSFSPDEKKEFERAYSAS+YPCMDILYECYEDVASGSEIRSVVLA
Sbjct: 361 IISKTISTKGMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLA 420

Query: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480
           GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI
Sbjct: 421 GRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEI 480

Query: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540
           LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ
Sbjct: 481 LRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQ 540

Query: 541 QALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQLSN 600
           QALVAIDNGSA+NQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ SN
Sbjct: 541 QALVAIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of HG10005176 vs. ExPASy TrEMBL
Match: A0A0A0K5T7 (Ketol-acid reductoisomerase OS=Cucumis sativus OX=3659 GN=Csa_7G051370 PE=3 SV=1)

HSP 1 Score: 1089.7 bits (2817), Expect = 0.0e+00
Identity = 561/595 (94.29%), Postives = 569/595 (95.63%), Query Frame = 0

Query: 2   AAATYFAPSISASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGAANPVGS 61
           AA T+FAPSISASS KTLK  AA  PLAPSSL FL  SSSSSG NALKAVGI AAN +GS
Sbjct: 3   AATTHFAPSISASSAKTLKHAAALHPLAPSSLSFL--SSSSSGLNALKAVGISAANGIGS 62

Query: 62  ALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRDLFPLLP 121
           ALGA+MVSVPAIKPL SLDFDTSVFKKEKVNLAGHDE             GGRDLFPLLP
Sbjct: 63  ALGARMVSVPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVR---------GGRDLFPLLP 122

Query: 122 EAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEE 181
           EAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEE
Sbjct: 123 EAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEE 182

Query: 182 NGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLD 241
           NGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLD
Sbjct: 183 NGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLD 242

Query: 242 FPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVALG 301
           FPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWS+ALG
Sbjct: 243 FPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIALG 302

Query: 302 SPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITGI 361
           SPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGM+E+LAYKNTVESITGI
Sbjct: 303 SPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGI 362

Query: 362 ISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAG 421
           IS+TISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAG
Sbjct: 363 ISRTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAG 422

Query: 422 RRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEIL 481
           RRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEIL
Sbjct: 423 RRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEIL 482

Query: 482 RKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ 541
           RKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ
Sbjct: 483 RKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQ 542

Query: 542 ALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ 597
           ALVAIDNGSA+NQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ
Sbjct: 543 ALVAIDNGSAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQ 586

BLAST of HG10005176 vs. TAIR 10
Match: AT3G58610.1 (ketol-acid reductoisomerase )

HSP 1 Score: 963.8 bits (2490), Expect = 6.6e-281
Identity = 504/604 (83.44%), Postives = 539/604 (89.24%), Query Frame = 0

Query: 1   MAAAT-YFAPSIS----ASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGA 60
           MAAAT   APS+S    +SS+KTL    A + LA  ++GFLSSSS S  S      G GA
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTLWSSKA-RTLALPNIGFLSSSSKSLRSLTATVAGNGA 60

Query: 61  ANPVGSALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRD 120
               GS+L A+MVS  A+K   SLDF+TSVFKKEKV+LAG++E             GGRD
Sbjct: 61  ---TGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVR---------GGRD 120

Query: 121 LFPLLPEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA 180
           LF  LP+AFKGIK+IGVIGWGSQ PAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EARA
Sbjct: 121 LFKHLPDAFKGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARA 180

Query: 181 VGFSEENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHL 240
            GF+EE+GTLGDI+ETI+ SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHL
Sbjct: 181 AGFTEESGTLGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHL 240

Query: 241 QSSGLDFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALG 300
           QSSGLDFPKNISV+AVCPKGMGPSVRRLYVQGKE+NGAGIN+SFAVHQDVDGRA DVALG
Sbjct: 241 QSSGLDFPKNISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALG 300

Query: 301 WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV 360
           WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV
Sbjct: 301 WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV 360

Query: 361 ESITGIISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIR 420
           E ITG IS+TIST+GMLAVYNS S + KK+FE AYSAS+YPCM+ILYECYEDV SGSEIR
Sbjct: 361 ECITGTISRTISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIR 420

Query: 421 SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMM 480
           SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVR+SRPAGDLGPLYPFTAGVYVALMM
Sbjct: 421 SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMM 480

Query: 481 AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD 540
           AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD
Sbjct: 481 AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD 540

Query: 541 YILTQQALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELR 600
           YILTQQALVA+D+G+AIN+DLISNF SDPVHGAIEVCAQLRPTVDISVPADADFVRPELR
Sbjct: 541 YILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELR 591

BLAST of HG10005176 vs. TAIR 10
Match: AT3G58610.2 (ketol-acid reductoisomerase )

HSP 1 Score: 963.8 bits (2490), Expect = 6.6e-281
Identity = 504/604 (83.44%), Postives = 539/604 (89.24%), Query Frame = 0

Query: 1   MAAAT-YFAPSIS----ASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGA 60
           MAAAT   APS+S    +SS+KTL    A + LA  ++GFLSSSS S  S      G GA
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTLWSSKA-RTLALPNIGFLSSSSKSLRSLTATVAGNGA 60

Query: 61  ANPVGSALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRD 120
               GS+L A+MVS  A+K   SLDF+TSVFKKEKV+LAG++E             GGRD
Sbjct: 61  ---TGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVR---------GGRD 120

Query: 121 LFPLLPEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA 180
           LF  LP+AFKGIK+IGVIGWGSQ PAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EARA
Sbjct: 121 LFKHLPDAFKGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARA 180

Query: 181 VGFSEENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHL 240
            GF+EE+GTLGDI+ETI+ SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHL
Sbjct: 181 AGFTEESGTLGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHL 240

Query: 241 QSSGLDFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALG 300
           QSSGLDFPKNISV+AVCPKGMGPSVRRLYVQGKE+NGAGIN+SFAVHQDVDGRA DVALG
Sbjct: 241 QSSGLDFPKNISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALG 300

Query: 301 WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV 360
           WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV
Sbjct: 301 WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV 360

Query: 361 ESITGIISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIR 420
           E ITG IS+TIST+GMLAVYNS S + KK+FE AYSAS+YPCM+ILYECYEDV SGSEIR
Sbjct: 361 ECITGTISRTISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIR 420

Query: 421 SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMM 480
           SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVR+SRPAGDLGPLYPFTAGVYVALMM
Sbjct: 421 SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMM 480

Query: 481 AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD 540
           AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD
Sbjct: 481 AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD 540

Query: 541 YILTQQALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELR 600
           YILTQQALVA+D+G+AIN+DLISNF SDPVHGAIEVCAQLRPTVDISVPADADFVRPELR
Sbjct: 541 YILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELR 591

BLAST of HG10005176 vs. TAIR 10
Match: AT3G58610.3 (ketol-acid reductoisomerase )

HSP 1 Score: 963.8 bits (2490), Expect = 6.6e-281
Identity = 504/604 (83.44%), Postives = 539/604 (89.24%), Query Frame = 0

Query: 1   MAAAT-YFAPSIS----ASSTKTLKQPAAFQPLAPSSLGFLSSSSSSSGSNALKAVGIGA 60
           MAAAT   APS+S    +SS+KTL    A + LA  ++GFLSSSS S  S      G GA
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTLWSSKA-RTLALPNIGFLSSSSKSLRSLTATVAGNGA 60

Query: 61  ANPVGSALGAQMVSVPAIKPLPSLDFDTSVFKKEKVNLAGHDEVFTPFTCNGFWGIGGRD 120
               GS+L A+MVS  A+K   SLDF+TSVFKKEKV+LAG++E             GGRD
Sbjct: 61  ---TGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVR---------GGRD 120

Query: 121 LFPLLPEAFKGIKKIGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA 180
           LF  LP+AFKGIK+IGVIGWGSQ PAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EARA
Sbjct: 121 LFKHLPDAFKGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARA 180

Query: 181 VGFSEENGTLGDIYETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHL 240
            GF+EE+GTLGDI+ETI+ SDLV+LLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHL
Sbjct: 181 AGFTEESGTLGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHL 240

Query: 241 QSSGLDFPKNISVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALG 300
           QSSGLDFPKNISV+AVCPKGMGPSVRRLYVQGKE+NGAGIN+SFAVHQDVDGRA DVALG
Sbjct: 241 QSSGLDFPKNISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALG 300

Query: 301 WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV 360
           WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV
Sbjct: 301 WSVALGSPFTFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTV 360

Query: 361 ESITGIISKTISTKGMLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIR 420
           E ITG IS+TIST+GMLAVYNS S + KK+FE AYSAS+YPCM+ILYECYEDV SGSEIR
Sbjct: 361 ECITGTISRTISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIR 420

Query: 421 SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMM 480
           SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVR+SRPAGDLGPLYPFTAGVYVALMM
Sbjct: 421 SVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMM 480

Query: 481 AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD 540
           AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD
Sbjct: 481 AQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFD 540

Query: 541 YILTQQALVAIDNGSAINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELR 600
           YILTQQALVA+D+G+AIN+DLISNF SDPVHGAIEVCAQLRPTVDISVPADADFVRPELR
Sbjct: 541 YILTQQALVAVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELR 591

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888114.10.0e+0095.33ketol-acid reductoisomerase, chloroplastic [Benincasa hispida][more]
XP_008455269.10.0e+0094.66PREDICTED: ketol-acid reductoisomerase, chloroplastic [Cucumis melo] >KAA0031534... [more]
XP_022952496.10.0e+0093.66ketol-acid reductoisomerase, chloroplastic-like [Cucurbita moschata][more]
XP_022969037.10.0e+0093.66ketol-acid reductoisomerase, chloroplastic-like [Cucurbita maxima][more]
XP_023554080.10.0e+0093.49ketol-acid reductoisomerase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q057589.3e-28083.44Ketol-acid reductoisomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At... [more]
O820431.2e-27481.30Ketol-acid reductoisomerase, chloroplastic OS=Pisum sativum OX=3888 GN=PGAAIR PE... [more]
Q012921.2e-27482.33Ketol-acid reductoisomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=AHRI ... [more]
Q65XK06.2e-26879.47Ketol-acid reductoisomerase, chloroplastic OS=Oryza sativa subsp. japonica OX=39... [more]
A8ZTR02.2e-3933.43Ketol-acid reductoisomerase (NAD(+)) OS=Desulfococcus oleovorans (strain DSM 620... [more]
Match NameE-valueIdentityDescription
A0A5D3C5630.0e+0094.66Ketol-acid reductoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3C0390.0e+0094.66Ketol-acid reductoisomerase OS=Cucumis melo OX=3656 GN=LOC103495472 PE=3 SV=1[more]
A0A6J1GKD70.0e+0093.66Ketol-acid reductoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111455167 PE=3 S... [more]
A0A6J1HV870.0e+0093.66Ketol-acid reductoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111468154 PE=3 SV=... [more]
A0A0A0K5T70.0e+0094.29Ketol-acid reductoisomerase OS=Cucumis sativus OX=3659 GN=Csa_7G051370 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G58610.16.6e-28183.44ketol-acid reductoisomerase [more]
AT3G58610.26.6e-28183.44ketol-acid reductoisomerase [more]
AT3G58610.36.6e-28183.44ketol-acid reductoisomerase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 304..577
e-value: 1.5E-157
score: 525.3
NoneNo IPR availableGENE3D3.40.50.720coord: 67..303
e-value: 1.3E-104
score: 350.4
NoneNo IPR availablePIRSRPIRSR000118-1PIRSR000118-1coord: 3..598
e-value: 0.0
score: 1202.1
NoneNo IPR availablePIRSRPIRSR000118-2PIRSR000118-2coord: 3..598
e-value: 0.0
score: 1202.1
NoneNo IPR availablePANTHERPTHR21371:SF20KETOL-ACID REDUCTOISOMERASEcoord: 9..598
IPR000506Ketol-acid reductoisomerase, C-terminalPFAMPF01450IlvCcoord: 311..455
e-value: 7.0E-34
score: 117.2
coord: 468..537
e-value: 1.3E-7
score: 32.0
IPR000506Ketol-acid reductoisomerase, C-terminalPROSITEPS51851KARI_Ccoord: 309..457
score: 32.639565
IPR000506Ketol-acid reductoisomerase, C-terminalPROSITEPS51851KARI_Ccoord: 458..594
score: 23.223982
IPR013116Ketol-acid reductoisomerase, N-terminalPFAMPF07991IlvNcoord: 128..302
e-value: 2.2E-31
score: 108.7
IPR013116Ketol-acid reductoisomerase, N-terminalPROSITEPS51850KARI_Ncoord: 111..308
score: 51.116451
IPR013023Ketol-acid reductoisomerasePANTHERPTHR21371KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIALcoord: 9..598
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 100..306
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 311..596

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10005176.1HG10005176.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009097 isoleucine biosynthetic process
biological_process GO:0009099 valine biosynthetic process
biological_process GO:0009082 branched-chain amino acid biosynthetic process
molecular_function GO:0016853 isomerase activity
molecular_function GO:0004455 ketol-acid reductoisomerase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity