HG10005014 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10005014
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionSqualene monooxygenase
LocationChr08: 22253025 .. 22256289 (+)
RNA-Seq ExpressionHG10005014
SyntenyHG10005014
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGATCACTGCGCCTTCGGCTGGATCTTGTCCGCCTTCCTCGCCTTCGCCATTGCCCTCTCTTTCTTCCTCGCTTCCAGGAGGAATCGCCGAGTTGGAGGAGCCGATTCCACGCCGAGGAGTGAATGTGTCAGAAGCTGCTCTGCATCCACCAACGGAGAATGCAGATCCGTCGACGGCGACGCTGACGTCATCATCGTCGGAGCCGGTGTTGCCGGTTCCGCTCTTGCTCATACTCTCGGCAAGGTACAGCGGTTTTATCGCTTTATTTTGAATTTTTAATCTCTTCTTTCTTATCAAGCATTTGATTATTATTATAATTGATATTTGATTCATAGAAACCCCTAGAATGAAAGAATCCATCGCAAATTTATGGATTGTTATGGATGAGAGGAAAAACAGTGGAAAGAAGAAAGAAATTACAAACTTCGGGTTGTAAATTGCTCTTCCTTCCATGTAACCAATGAAATGACTGGATTATTGTTTTTTTCTTTCTTTATTTCTTATTGGTGCAAATATATAGAATTAAAATTTTATGCAGCCACCAGTTGATTTTTATAATGGATAAAATCGCTGCTTCGTATTTTGATGTAAAATGGTTTTGATTATTGTTGACCCAAGTACAAGGTCCGAATTTAAATTTTAAATCTTAACCCAAAACCCAATTTATTTGTTTATTTATTTTATGTGATTGTAGTTCATCATCTATTACCCTTTCAAAGGTCAATTATTATTTTAAAAATTTGATGGTTAAATTTAGAAGGAAAAAAAAAATGGTTTATATATATATATTTTCTCTTTTTGTTATTTTTACAATGGAAGCAAAGCAAGCTGATGAGATCAAAATTGGTAGGATGGCCGTCGAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGAATCGTCGGTGAATTATTGCAGCCTGGGGGCTACCTCAAATTGATTGAGTTAGGACTGCAAGGTTTGTAATTACCCATGGCAAATTTCTTTTCCAACTCTATTTTTTTTATTTCTTAAAAGTACAAAATATTCATCAATTAAAATAGGAAAACACATGTTCGTAATACAGATTGCGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCCCTTTTCAAGGATGGAAAGAGCACTCGACTTTCTTACCCATTGGAAAATTTTCACTCTGATGTATCTGGAAGAAGCTTCCACAATGGACGCTTCATACAGAGAATGAGGGAGAAGGCTGCTTCACTTCCCAAGTACGTTCATTTCGTCTTTGTTTTCTGCTCCTTGAACTCTTAAGAGTATGAGTTTTAGTTAAACAAGTAAATTATTTGTAACCCATCAACTTAAGTTTTTGAGTTAGTTGGTGATTTTAACCCATTTAACCCCTATATAATGTCCCATGATCAGATGTTAAATGTTAATTTTGGTCTCTTGAAGTAAAGATTTTGTGAACCGGAGTGTTTTGACAATTTATGTCCTGAGTTGTTCTGTGATATGAAACTCTTGTGCTCTTGAAATGTTAGAGATTTGACTGTATTGTTAAGCAGATTCGGACAAAATGAGGTTGAAATTAATGATACTTCTACTTATGTAAATGGATTTAAGACAAATTTCCTAGGTGTGGTTTAGAGCCGCCCTATTGAATCTAGTTGAAGTCATAGTGACAAACATTCACCATTTATACTTGTGTATTTCCTGTGATTGGATGAATGAATAACAACTACTTAGGTTTCACTCATCAATGAAAATTTGTACATGCTCTGTTTCCGTTTCTTGTAGTGTGAGATTGGAGCAAGGGACAGTTACTTCATTGCTTGAAGAAAAGGGAACGATCAAAGGTGTGCAGTATAAATCCAAAAATGGTGAAGAAAAAACAGCATATGCTCCTCTGACCATTGTTTGTGATGGCTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAAACCCATGGTAAATAGGCTTCAATTATGCAGTGGAACAGGCATTGATTTTCTCTTGGGATTTGATTGGCTGCAGAACTATTCTAATTACATTTTGCCAACTGCATGAAAACAGTGAATAAGTTTTTAAATTCCGCCCTTTACCTGATCTATTTTTTGCAGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCTTGGGCATGTTGTTCTTGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGCAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTACTTGAAGACCGTAGTAGCTCCTCAGGTACAATTTTTTCAACAGTCCCATTCATGCCAATGTATGTGTTCTAGTGCTCTGAACTGATTAATTGACCATTACTCGTGCAGGTTCCTCCGGAAATCCATGATGCATTCATAGCTGCTATTGAGAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCCGCTCCCCAGCCGACACCTGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCTCTCACTGGTGGAGGAATGACTGTAGCTTTGTCTGATATTGTTGTGCTCAGGAACCTCCTAAAGCCATTGAAAAACTTGAATGATGCACCAACTCTCTGCAAATATCTAGAATCCTTTTACACTCTGAGAAAGGTATAACTTTAGTTTACGTTGTTGTTGGCAATCCTAAAAGCCATTTTACAACAGGCCGTAAGTAGAAGCTGCTCTAATATATTCGTCTCAAACTGCAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTGTTTTGTGCATCGCCAGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCTGTGTCCTTGCTTTCGGGATTGAATCCTCGCCCATTGAGTCTCGTTCTCCATTTCTTTGCGGTTGCAATATATGGAGTTGGACGCTTACTGCTTCCATTTCCTTCTCTGAAAGGCATCTGGATTGGAGCAAGATTGGTCTATGTGAGTTTTGAACAAACAAAACACCTTCATTCATTAATATTTTCCTCTTAGACAAGTTATGAGTAATGAGAAATGAATTGGTTTGTTTATTGCAGAGCGCATCAGGAATCATATTTCCAATAATAAAAGCGGAAGGAGTTAGGCAGATGTTCTTCCCAGCAACTGTTCCTGCTTATTACAGAAGCCCACCAGTTTTTAAACCCATAGCATGA

mRNA sequence

ATGGTGGATCACTGCGCCTTCGGCTGGATCTTGTCCGCCTTCCTCGCCTTCGCCATTGCCCTCTCTTTCTTCCTCGCTTCCAGGAGGAATCGCCGAGTTGGAGGAGCCGATTCCACGCCGAGGAGTGAATGTGTCAGAAGCTGCTCTGCATCCACCAACGGAGAATGCAGATCCGTCGACGGCGACGCTGACGTCATCATCGTCGGAGCCGGTGTTGCCGGTTCCGCTCTTGCTCATACTCTCGGCAAGGATGGCCGTCGAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGAATCGTCGGTGAATTATTGCAGCCTGGGGGCTACCTCAAATTGATTGAGTTAGGACTGCAAGATTGCGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCCCTTTTCAAGGATGGAAAGAGCACTCGACTTTCTTACCCATTGGAAAATTTTCACTCTGATGTATCTGGAAGAAGCTTCCACAATGGACGCTTCATACAGAGAATGAGGGAGAAGGCTGCTTCACTTCCCAATGTGAGATTGGAGCAAGGGACAGTTACTTCATTGCTTGAAGAAAAGGGAACGATCAAAGGTGTGCAGTATAAATCCAAAAATGGTGAAGAAAAAACAGCATATGCTCCTCTGACCATTGTTTGTGATGGCTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAAACCCATGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCTTGGGCATGTTGTTCTTGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGCAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTACTTGAAGACCGTAGTAGCTCCTCAGGTTCCTCCGGAAATCCATGATGCATTCATAGCTGCTATTGAGAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCCGCTCCCCAGCCGACACCTGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCTCTCACTGGTGGAGGAATGACTGTAGCTTTGTCTGATATTGTTGTGCTCAGGAACCTCCTAAAGCCATTGAAAAACTTGAATGATGCACCAACTCTCTGCAAATATCTAGAATCCTTTTACACTCTGAGAAAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTGTTTTGTGCATCGCCAGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCTGTGTCCTTGCTTTCGGGATTGAATCCTCGCCCATTGAGTCTCGTTCTCCATTTCTTTGCGGTTGCAATATATGGAGTTGGACGCTTACTGCTTCCATTTCCTTCTCTGAAAGGCATCTGGATTGGAGCAAGATTGGTCTATAGCGCATCAGGAATCATATTTCCAATAATAAAAGCGGAAGGAGTTAGGCAGATGTTCTTCCCAGCAACTGTTCCTGCTTATTACAGAAGCCCACCAGTTTTTAAACCCATAGCATGA

Coding sequence (CDS)

ATGGTGGATCACTGCGCCTTCGGCTGGATCTTGTCCGCCTTCCTCGCCTTCGCCATTGCCCTCTCTTTCTTCCTCGCTTCCAGGAGGAATCGCCGAGTTGGAGGAGCCGATTCCACGCCGAGGAGTGAATGTGTCAGAAGCTGCTCTGCATCCACCAACGGAGAATGCAGATCCGTCGACGGCGACGCTGACGTCATCATCGTCGGAGCCGGTGTTGCCGGTTCCGCTCTTGCTCATACTCTCGGCAAGGATGGCCGTCGAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGAATCGTCGGTGAATTATTGCAGCCTGGGGGCTACCTCAAATTGATTGAGTTAGGACTGCAAGATTGCGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCCCTTTTCAAGGATGGAAAGAGCACTCGACTTTCTTACCCATTGGAAAATTTTCACTCTGATGTATCTGGAAGAAGCTTCCACAATGGACGCTTCATACAGAGAATGAGGGAGAAGGCTGCTTCACTTCCCAATGTGAGATTGGAGCAAGGGACAGTTACTTCATTGCTTGAAGAAAAGGGAACGATCAAAGGTGTGCAGTATAAATCCAAAAATGGTGAAGAAAAAACAGCATATGCTCCTCTGACCATTGTTTGTGATGGCTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAAACCCATGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCTTGGGCATGTTGTTCTTGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGCAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTACTTGAAGACCGTAGTAGCTCCTCAGGTTCCTCCGGAAATCCATGATGCATTCATAGCTGCTATTGAGAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCCGCTCCCCAGCCGACACCTGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCTCTCACTGGTGGAGGAATGACTGTAGCTTTGTCTGATATTGTTGTGCTCAGGAACCTCCTAAAGCCATTGAAAAACTTGAATGATGCACCAACTCTCTGCAAATATCTAGAATCCTTTTACACTCTGAGAAAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTGTTTTGTGCATCGCCAGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCTGTGTCCTTGCTTTCGGGATTGAATCCTCGCCCATTGAGTCTCGTTCTCCATTTCTTTGCGGTTGCAATATATGGAGTTGGACGCTTACTGCTTCCATTTCCTTCTCTGAAAGGCATCTGGATTGGAGCAAGATTGGTCTATAGCGCATCAGGAATCATATTTCCAATAATAAAAGCGGAAGGAGTTAGGCAGATGTTCTTCCCAGCAACTGTTCCTGCTTATTACAGAAGCCCACCAGTTTTTAAACCCATAGCATGA

Protein sequence

MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVDGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFKPIA
Homology
BLAST of HG10005014 vs. NCBI nr
Match: XP_038886247.1 (squalene monooxygenase SE1-like [Benincasa hispida])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.6e-293
Identity = 507/530 (95.66%), Postives = 517/530 (97.55%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVDHCAFGWILSA  AF IALSFFLA RRNRRV G  STPRS+C+RS S++TNGECRS+D
Sbjct: 1   MVDHCAFGWILSALFAFVIALSFFLAPRRNRRVEGTHSTPRSDCLRS-SSTTNGECRSID 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
           GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGK+TRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTIKGVQYKSKNGE KTAYAPLTIVCDGCFSNLRRSLC PMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEVKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
           CFVGLVLENCQLPFAN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY
Sbjct: 241 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKTVVAPQVP EIHDAF+AAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTVVAPQVPREIHDAFVAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
           PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFKPI 531
           S KGIWIGARL+YSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFKP+
Sbjct: 481 SPKGIWIGARLIYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFKPM 529

BLAST of HG10005014 vs. NCBI nr
Match: XP_022996831.1 (squalene monooxygenase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.2e-289
Identity = 504/531 (94.92%), Postives = 517/531 (97.36%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVD+CAFGWIL+A L  AIALSFF++ RRNRR GGADSTPRSE VRS S++TNGECRSVD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRR-GGADSTPRSEGVRS-SSTTNGECRSVD 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
           GDADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  GDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGK+T+LSYPLE F SDVSGRSFHNGRFIQRMREKAASLPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
           CFVGLVLENCQLPFAN GHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSISNGEMEKY
Sbjct: 241 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKT+VAPQVPP+IHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
           PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFKPIA 532
           S KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV K IA
Sbjct: 481 SPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSIA 529

BLAST of HG10005014 vs. NCBI nr
Match: XP_008444517.1 (PREDICTED: squalene monooxygenase-like [Cucumis melo])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.1e-289
Identity = 502/526 (95.44%), Postives = 511/526 (97.15%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVDHCAFGWI SA LAF IALS FL+  RNRRV G DSTPRS  V S SA+TNGECRSVD
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASV-SSSATTNGECRSVD 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
           GDADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  GDADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGK+TRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTI GVQYKSKNGE+KTAYAPLTIVCDGCFSNLRRSLC PMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
            FVGLVLENCQLP+ANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY
Sbjct: 241 YFVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
           PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV 527
           SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV
Sbjct: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 525

BLAST of HG10005014 vs. NCBI nr
Match: KAA0060974.1 (squalene monooxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.1e-289
Identity = 502/526 (95.44%), Postives = 511/526 (97.15%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVDHCAFGWI SA LAF IALS FL+  RNRRV G DSTPRS  V S SA+TNGECRSVD
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASV-SSSATTNGECRSVD 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
           GDADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  GDADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGK+TRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTI GVQYKSKNGE+KTAYAPLTIVCDGCFSNLRRSLC PMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
            FVGLVLENCQLP+ANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY
Sbjct: 241 FFVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
           PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV 527
           SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV
Sbjct: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 525

BLAST of HG10005014 vs. NCBI nr
Match: KAG6598316.1 (Squalene monooxygenase SE2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029287.1 hypothetical protein SDJN02_07625 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1003.0 bits (2592), Expect = 9.3e-289
Identity = 504/531 (94.92%), Postives = 517/531 (97.36%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVD+CAFGWIL+A L  AIALSFF++ RRNRR GGADSTPRSE VRS S++TNGECRSVD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRR-GGADSTPRSEGVRS-SSTTNGECRSVD 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
           GDADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  GDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGK+T+LSYPLE F SDVSGRSFHNGRFIQRMREKAASLPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTA+APLTIVCDGCFSNLRRSLCKPMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAHAPLTIVCDGCFSNLRRSLCKPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
           CFVGLVLENCQLPFAN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY
Sbjct: 241 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKT+VAPQVPP+IHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
           PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFKPIA 532
           S KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV K IA
Sbjct: 481 SPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of HG10005014 vs. ExPASy Swiss-Prot
Match: B7TWW5 (Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2)

HSP 1 Score: 801.6 bits (2069), Expect = 5.4e-231
Identity = 399/528 (75.57%), Postives = 451/528 (85.42%), Query Frame = 0

Query: 10  ILSAFLAFAIALSFFLASRRNRR---VGGADSTP--RSECVRSCSASTNGECRSVD---G 69
           + +A L F   L  F   RR RR    GGA S          + S+  NG C   D    
Sbjct: 16  MFAATLLFGFVLYLFTLRRRRRRREKKGGAGSMEIINGAYKMTSSSEVNGHCTPEDIAGS 75

Query: 70  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 129
             DVIIVGAGVAGSALA+TL KDGRRVHVIERDLTE DRIVGELLQPGGYLKL+ELGL+D
Sbjct: 76  SDDVIIVGAGVAGSALAYTLAKDGRRVHVIERDLTEQDRIVGELLQPGGYLKLVELGLED 135

Query: 130 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 189
           CV EIDAQ+V+GYAL+ DGK+TRLSYPLE FH+DV+GRSFHNGRFIQRMREKAASLPNVR
Sbjct: 136 CVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHADVAGRSFHNGRFIQRMREKAASLPNVR 195

Query: 190 LEQGTVTSLLEEKGTIKGVQYKSKNGEE-KTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 249
           +EQGTVTSL+E+KGT+KGV+YK+KNG+E   AYAPLTIVCDGCFSNLR SLC P VDVPS
Sbjct: 196 MEQGTVTSLVEQKGTVKGVRYKTKNGQEMSAAYAPLTIVCDGCFSNLRHSLCNPKVDVPS 255

Query: 250 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 309
           CFVGL+LEN  LP  N GHV+L DPSPILFY ISSTEIRCLVDVPGQKVPSI+NGE+  Y
Sbjct: 256 CFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPSIANGELAHY 315

Query: 310 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 369
           LKT VAPQ+PPE++ +FIAAI+KG I+TMPNRSMPA P  TPGALL+GDAFNMRHPLTGG
Sbjct: 316 LKTSVAPQIPPELYKSFIAAIDKGKIKTMPNRSMPADPHSTPGALLLGDAFNMRHPLTGG 375

Query: 370 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 429
           GMTVALSDIV++R+LL+PL++L+D+ TLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 376 GMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 435

Query: 430 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 489
           PD+AR+EMR ACFDYLSLGGI S GP++LLSGLNPRP+SL  HFFAVAIYGVGRLL+PFP
Sbjct: 436 PDKARQEMRDACFDYLSLGGICSEGPIALLSGLNPRPMSLFFHFFAVAIYGVGRLLIPFP 495

Query: 490 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFK 529
           S + +W+GARL+  ASGIIFPIIK+EGVRQMFFPATVPAYYR+PP+ K
Sbjct: 496 SPRKMWLGARLISGASGIIFPIIKSEGVRQMFFPATVPAYYRAPPITK 543

BLAST of HG10005014 vs. ExPASy Swiss-Prot
Match: O48651 (Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 7.1e-231
Identity = 392/528 (74.24%), Postives = 455/528 (86.17%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNG--ECRS 60
           ++D    GWI +    F + L+F    +R R         ++      +  +NG     +
Sbjct: 23  LIDQYFLGWIFAFLFGFLLLLNF----KRKRE--------KNNSTEFGTDDSNGYYTPEN 82

Query: 61  VDGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELG 120
           + G  DVIIVGAGVAGSALA+TL  DGRRVHVIERDLTE DRIVGELLQPGGYLKLIELG
Sbjct: 83  IAGSTDVIIVGAGVAGSALAYTLANDGRRVHVIERDLTEQDRIVGELLQPGGYLKLIELG 142

Query: 121 LQDCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLP 180
           L+DCV EIDAQ+V+GYAL+ DGK+TRLSYPLE FHSDV+GRSFHNGRF+QRMREKAASLP
Sbjct: 143 LEDCVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHSDVAGRSFHNGRFVQRMREKAASLP 202

Query: 181 NVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240
           NVR+EQGTVTSL+E+KG++KGVQYK+K+G+E +A+APLTIVCDGCFSNLRRSLC P V+V
Sbjct: 203 NVRMEQGTVTSLVEKKGSVKGVQYKTKDGQELSAFAPLTIVCDGCFSNLRRSLCNPKVEV 262

Query: 241 PSCFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEME 300
           PSCFVGL+LEN  LP  N GHV+L DPSPILFY ISSTEIRCLVDVPGQKVP ISNGE+ 
Sbjct: 263 PSCFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPCISNGELA 322

Query: 301 KYLKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLT 360
            YLKTVVAPQVP +++++FIAA++KGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLT
Sbjct: 323 NYLKTVVAPQVPKQLYNSFIAAVDKGNIRTMPNRSMPADPHPTPGALLLGDAFNMRHPLT 382

Query: 361 GGGMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFC 420
           GGGMTVALSDIV++R+LL+PL++L+D+ TLCKYLESFYTLRKPVASTINTLAGALYKVFC
Sbjct: 383 GGGMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFC 442

Query: 421 ASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLP 480
           ASPD+AR+EMR ACFDYLSLGGI S GP++LLSGLNPRP+SL LHFFAVAIYGVGRLL+P
Sbjct: 443 ASPDKARQEMRNACFDYLSLGGICSQGPIALLSGLNPRPISLFLHFFAVAIYGVGRLLIP 502

Query: 481 FPSLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV 527
           FPS K +W+GARL+  ASGIIFPIIK+EG+RQMFFPA VPAYYR+PP+
Sbjct: 503 FPSPKRMWLGARLILGASGIIFPIIKSEGLRQMFFPAIVPAYYRAPPI 538

BLAST of HG10005014 vs. ExPASy Swiss-Prot
Match: Q9SM02 (Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 4.3e-228
Identity = 396/526 (75.29%), Postives = 451/526 (85.74%), Query Frame = 0

Query: 7   FGWIL-----SAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVDG 66
           + WIL     S  ++F     FF+  +RN   G          V S   S N     + G
Sbjct: 6   WNWILPLLISSLLISFVAFYGFFVKPKRN---GLRHDRKTVSTVTSDVGSVN-----ITG 65

Query: 67  D--ADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 126
           D  ADVI+VGAGVAGSALA+TLGKD RRVHVIERDL+EPDRIVGELLQPGGYLKL+ELG+
Sbjct: 66  DTVADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGI 125

Query: 127 QDCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPN 186
           +DCVEEIDAQ+VYGYALFK+GK  RL+YPLE FH DVSGRSFHNGRFIQRMREKAASLPN
Sbjct: 126 EDCVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPN 185

Query: 187 VRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 246
           V+LEQGTV SLLEE GTIKGV+YK+K GEE+TA+A LTIVCDGCFSNLRRSLC P V+VP
Sbjct: 186 VQLEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVP 245

Query: 247 SCFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEK 306
           SCFVGLVLENC LP+AN GHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI+NGEM+ 
Sbjct: 246 SCFVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKN 305

Query: 307 YLKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTG 366
           YLKTVVAPQ+P E++D+FIAA++KGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTG
Sbjct: 306 YLKTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTG 365

Query: 367 GGMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCA 426
           GGMTVAL+DIVVLRNLL+PL++L+D  +LCKYLESFYTLRKPVA+TINTLA ALY+VFC+
Sbjct: 366 GGMTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCS 425

Query: 427 SPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPF 486
           S ++AR EMR+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PF
Sbjct: 426 SENEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPF 485

Query: 487 PSLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 526
           PS K IW+GA+L+  ASGIIFPIIKAEGVRQMFFPATVPAYY   P
Sbjct: 486 PSPKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of HG10005014 vs. ExPASy Swiss-Prot
Match: Q8VYH2 (Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.5e-225
Identity = 390/525 (74.29%), Postives = 447/525 (85.14%), Query Frame = 0

Query: 2   VDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVDG 61
           VDHC    + + F+A   A       RR           RS+ +       NG      G
Sbjct: 7   VDHCI---LTTTFVASLFAFLLLYVLRR-----------RSKTIHGSVNVRNGTLTVKSG 66

Query: 62  -DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 121
            D D+IIVGAGVAG+ALAHTLGK+GRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL+
Sbjct: 67  TDVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLE 126

Query: 122 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 181
           DCV++IDAQ+V GYALFKDGK T+LSYPL+ F SDV+GRSFHNGRF+QRMREKA+ LPNV
Sbjct: 127 DCVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNV 186

Query: 182 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 241
           R+EQGTVTSL+EE G IKGVQYK+K+G+E  ++APLTIVCDGCFSNLRRSLCKP V+VPS
Sbjct: 187 RMEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPS 246

Query: 242 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 301
            FVGLVLENC+LPF N GHVVLGDPSPILFYPISS+E+RCLVDVPG K+PS+++GEM  +
Sbjct: 247 NFVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHH 306

Query: 302 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 361
           LKT+VAPQVPP+I DAFI+A+EKGNIRTMPNRSMPA P  TPGALL+GDAFNMRHPLTGG
Sbjct: 307 LKTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGG 366

Query: 362 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 421
           GMTVALSDIV+LR+LL PL +L +  +L KY+ESFYTLRKPVASTINTLAGALYKVF AS
Sbjct: 367 GMTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLAS 426

Query: 422 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 481
           PD AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+SLVLHFFAVAI+GVGRLL+P P
Sbjct: 427 PDDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLP 486

Query: 482 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 526
           S+K +W+GARL+ SASGIIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 487 SVKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of HG10005014 vs. ExPASy Swiss-Prot
Match: O81000 (Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 4.1e-223
Identity = 385/509 (75.64%), Postives = 433/509 (85.07%), Query Frame = 0

Query: 17  FAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVDGDADVIIVGAGVAGSA 76
           F  +L  FL     RR    +   R   V      +      VD   DVIIVGAGVAGSA
Sbjct: 77  FIASLFTFLLLYILRRSSNKNKKNRGLVVSQNDTVSKNLETEVDSGTDVIIVGAGVAGSA 136

Query: 77  LAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYAL 136
           LAHTLGK+GRRVHVIERD +E DRIVGELLQPGGYLKLIELGL+DCV++IDAQ+V GY L
Sbjct: 137 LAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKIDAQRVLGYVL 196

Query: 137 FKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVRLEQGTVTSLLEEKGT 196
           FKDGK T+L+YPLE F SDV+GRSFHNGRF+QRMREKA +L NVRLEQGTVTSLLEE GT
Sbjct: 197 FKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGTVTSLLEEHGT 256

Query: 197 IKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCFVGLVLENCQLPFAN 256
           IKGV+Y++K G E  ++APLTIVCDGCFSNLRRSLCKP VDVPS FVGLVLENC+LPFAN
Sbjct: 257 IKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLVLENCELPFAN 316

Query: 257 LGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPEIHDA 316
            GHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM KYLKT VAPQVP ++ +A
Sbjct: 317 HGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVAPQVPTKVREA 376

Query: 317 FIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLL 376
           FI A+EKGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLTGGGMTVAL+DIVVLR+LL
Sbjct: 377 FITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLL 436

Query: 377 KPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDQARKEMRQACFDYL 436
           +P++NLND   L KY+ESFYTLRKPVASTINTLA ALYKVF AS D+AR EMR+ACFDYL
Sbjct: 437 RPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFLASSDEARTEMREACFDYL 496

Query: 437 SLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSLKGIWIGARLVYSAS 496
           SLGG+FS+GPV+LLSGLNPRPLSLVLHFFAVAIY V RL+LPFPS++  W+GAR++ SAS
Sbjct: 497 SLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFWLGARIISSAS 556

Query: 497 GIIFPIIKAEGVRQMFFPATVPAYYRSPP 526
            IIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 557 SIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of HG10005014 vs. ExPASy TrEMBL
Match: A0A6J1K5U9 (Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491959 PE=3 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 2.0e-289
Identity = 504/531 (94.92%), Postives = 517/531 (97.36%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVD+CAFGWIL+A L  AIALSFF++ RRNRR GGADSTPRSE VRS S++TNGECRSVD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRR-GGADSTPRSEGVRS-SSTTNGECRSVD 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
           GDADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  GDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGK+T+LSYPLE F SDVSGRSFHNGRFIQRMREKAASLPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
           CFVGLVLENCQLPFAN GHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSISNGEMEKY
Sbjct: 241 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKT+VAPQVPP+IHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
           PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFKPIA 532
           S KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV K IA
Sbjct: 481 SPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSIA 529

BLAST of HG10005014 vs. ExPASy TrEMBL
Match: A0A5A7V0X5 (Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001090 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.5e-289
Identity = 502/526 (95.44%), Postives = 511/526 (97.15%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVDHCAFGWI SA LAF IALS FL+  RNRRV G DSTPRS  V S SA+TNGECRSVD
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASV-SSSATTNGECRSVD 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
           GDADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  GDADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGK+TRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTI GVQYKSKNGE+KTAYAPLTIVCDGCFSNLRRSLC PMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
            FVGLVLENCQLP+ANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY
Sbjct: 241 FFVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
           PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV 527
           SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV
Sbjct: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 525

BLAST of HG10005014 vs. ExPASy TrEMBL
Match: A0A1S3BB88 (Squalene monooxygenase OS=Cucumis melo OX=3656 GN=LOC103487810 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.5e-289
Identity = 502/526 (95.44%), Postives = 511/526 (97.15%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVDHCAFGWI SA LAF IALS FL+  RNRRV G DSTPRS  V S SA+TNGECRSVD
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASV-SSSATTNGECRSVD 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
           GDADV+IVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  GDADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGK+TRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTI GVQYKSKNGE+KTAYAPLTIVCDGCFSNLRRSLC PMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
            FVGLVLENCQLP+ANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY
Sbjct: 241 YFVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
           PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV 527
           SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV
Sbjct: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPV 525

BLAST of HG10005014 vs. ExPASy TrEMBL
Match: A0A0A0LKI1 (Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G353480 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.0e-288
Identity = 499/527 (94.69%), Postives = 510/527 (96.77%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVDHC FGWI SAFLAF IA SFFL+ R+NRR  G +STPR +C+ S SA+TNGECRSVD
Sbjct: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCL-SSSATTNGECRSVD 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
           GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGKSTRLSYPLENF SDVSGRSFHNGRFIQRMREKAA LPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTI GVQYKSKNGE+KTAYAPLTIVCDGCFSNLRRSLC PMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
           CFVGLVLENCQLP+ANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY
Sbjct: 241 CFVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKTVVAPQVPP+IHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
            DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 SDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVF 528
           S KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPVF
Sbjct: 481 SPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVF 526

BLAST of HG10005014 vs. ExPASy TrEMBL
Match: A0A6J1HEG7 (Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111462746 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.0e-288
Identity = 503/531 (94.73%), Postives = 516/531 (97.18%), Query Frame = 0

Query: 1   MVDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVD 60
           MVD+CAFGWIL+A L  AIALSFF++ RRNRR GGADSTPRSE VRS S++TNGECRSVD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRR-GGADSTPRSEGVRS-SSTTNGECRSVD 60

Query: 61  GDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120
            DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ
Sbjct: 61  CDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 120

Query: 121 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 180
           DCVEEIDAQKVYGYALFKDGK+T+LSYPLE F SDVSGRSFHNGRFIQRMREKAASLPNV
Sbjct: 121 DCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNV 180

Query: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 240
           RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTA+APLTIVCDGCFSNLRRSLCKPMVDVPS
Sbjct: 181 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAHAPLTIVCDGCFSNLRRSLCKPMVDVPS 240

Query: 241 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300
           CFVGLVLENCQLPFAN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY
Sbjct: 241 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 300

Query: 301 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360
           LKT+VAPQVPP+IHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG
Sbjct: 301 LKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVALSDIVVLRNLLKPLK+LNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 361 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 420

Query: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480
           PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP
Sbjct: 421 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 480

Query: 481 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVFKPIA 532
           S KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV K IA
Sbjct: 481 SPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of HG10005014 vs. TAIR 10
Match: AT1G58440.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 792.0 bits (2044), Expect = 3.0e-229
Identity = 396/526 (75.29%), Postives = 451/526 (85.74%), Query Frame = 0

Query: 7   FGWIL-----SAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVDG 66
           + WIL     S  ++F     FF+  +RN   G          V S   S N     + G
Sbjct: 6   WNWILPLLISSLLISFVAFYGFFVKPKRN---GLRHDRKTVSTVTSDVGSVN-----ITG 65

Query: 67  D--ADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 126
           D  ADVI+VGAGVAGSALA+TLGKD RRVHVIERDL+EPDRIVGELLQPGGYLKL+ELG+
Sbjct: 66  DTVADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGI 125

Query: 127 QDCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPN 186
           +DCVEEIDAQ+VYGYALFK+GK  RL+YPLE FH DVSGRSFHNGRFIQRMREKAASLPN
Sbjct: 126 EDCVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPN 185

Query: 187 VRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 246
           V+LEQGTV SLLEE GTIKGV+YK+K GEE+TA+A LTIVCDGCFSNLRRSLC P V+VP
Sbjct: 186 VQLEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVP 245

Query: 247 SCFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEK 306
           SCFVGLVLENC LP+AN GHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI+NGEM+ 
Sbjct: 246 SCFVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKN 305

Query: 307 YLKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTG 366
           YLKTVVAPQ+P E++D+FIAA++KGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTG
Sbjct: 306 YLKTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTG 365

Query: 367 GGMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCA 426
           GGMTVAL+DIVVLRNLL+PL++L+D  +LCKYLESFYTLRKPVA+TINTLA ALY+VFC+
Sbjct: 366 GGMTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCS 425

Query: 427 SPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPF 486
           S ++AR EMR+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PF
Sbjct: 426 SENEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPF 485

Query: 487 PSLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 526
           PS K IW+GA+L+  ASGIIFPIIKAEGVRQMFFPATVPAYY   P
Sbjct: 486 PSPKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of HG10005014 vs. TAIR 10
Match: AT4G37760.1 (squalene epoxidase 3 )

HSP 1 Score: 783.5 bits (2022), Expect = 1.1e-226
Identity = 390/525 (74.29%), Postives = 447/525 (85.14%), Query Frame = 0

Query: 2   VDHCAFGWILSAFLAFAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVDG 61
           VDHC    + + F+A   A       RR           RS+ +       NG      G
Sbjct: 7   VDHCI---LTTTFVASLFAFLLLYVLRR-----------RSKTIHGSVNVRNGTLTVKSG 66

Query: 62  -DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQ 121
            D D+IIVGAGVAG+ALAHTLGK+GRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL+
Sbjct: 67  TDVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLE 126

Query: 122 DCVEEIDAQKVYGYALFKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNV 181
           DCV++IDAQ+V GYALFKDGK T+LSYPL+ F SDV+GRSFHNGRF+QRMREKA+ LPNV
Sbjct: 127 DCVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNV 186

Query: 182 RLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 241
           R+EQGTVTSL+EE G IKGVQYK+K+G+E  ++APLTIVCDGCFSNLRRSLCKP V+VPS
Sbjct: 187 RMEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPS 246

Query: 242 CFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 301
            FVGLVLENC+LPF N GHVVLGDPSPILFYPISS+E+RCLVDVPG K+PS+++GEM  +
Sbjct: 247 NFVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHH 306

Query: 302 LKTVVAPQVPPEIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 361
           LKT+VAPQVPP+I DAFI+A+EKGNIRTMPNRSMPA P  TPGALL+GDAFNMRHPLTGG
Sbjct: 307 LKTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGG 366

Query: 362 GMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 421
           GMTVALSDIV+LR+LL PL +L +  +L KY+ESFYTLRKPVASTINTLAGALYKVF AS
Sbjct: 367 GMTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLAS 426

Query: 422 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 481
           PD AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+SLVLHFFAVAI+GVGRLL+P P
Sbjct: 427 PDDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLP 486

Query: 482 SLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 526
           S+K +W+GARL+ SASGIIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 487 SVKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of HG10005014 vs. TAIR 10
Match: AT2G22830.1 (squalene epoxidase 2 )

HSP 1 Score: 775.4 bits (2001), Expect = 2.9e-224
Identity = 385/509 (75.64%), Postives = 433/509 (85.07%), Query Frame = 0

Query: 17  FAIALSFFLASRRNRRVGGADSTPRSECVRSCSASTNGECRSVDGDADVIIVGAGVAGSA 76
           F  +L  FL     RR    +   R   V      +      VD   DVIIVGAGVAGSA
Sbjct: 77  FIASLFTFLLLYILRRSSNKNKKNRGLVVSQNDTVSKNLETEVDSGTDVIIVGAGVAGSA 136

Query: 77  LAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYAL 136
           LAHTLGK+GRRVHVIERD +E DRIVGELLQPGGYLKLIELGL+DCV++IDAQ+V GY L
Sbjct: 137 LAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKIDAQRVLGYVL 196

Query: 137 FKDGKSTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVRLEQGTVTSLLEEKGT 196
           FKDGK T+L+YPLE F SDV+GRSFHNGRF+QRMREKA +L NVRLEQGTVTSLLEE GT
Sbjct: 197 FKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGTVTSLLEEHGT 256

Query: 197 IKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCFVGLVLENCQLPFAN 256
           IKGV+Y++K G E  ++APLTIVCDGCFSNLRRSLCKP VDVPS FVGLVLENC+LPFAN
Sbjct: 257 IKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLVLENCELPFAN 316

Query: 257 LGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPEIHDA 316
            GHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM KYLKT VAPQVP ++ +A
Sbjct: 317 HGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVAPQVPTKVREA 376

Query: 317 FIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLL 376
           FI A+EKGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLTGGGMTVAL+DIVVLR+LL
Sbjct: 377 FITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLL 436

Query: 377 KPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDQARKEMRQACFDYL 436
           +P++NLND   L KY+ESFYTLRKPVASTINTLA ALYKVF AS D+AR EMR+ACFDYL
Sbjct: 437 RPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFLASSDEARTEMREACFDYL 496

Query: 437 SLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSLKGIWIGARLVYSAS 496
           SLGG+FS+GPV+LLSGLNPRPLSLVLHFFAVAIY V RL+LPFPS++  W+GAR++ SAS
Sbjct: 497 SLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFWLGARIISSAS 556

Query: 497 GIIFPIIKAEGVRQMFFPATVPAYYRSPP 526
            IIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 557 SIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of HG10005014 vs. TAIR 10
Match: AT5G24150.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 505.4 bits (1300), Expect = 5.7e-143
Identity = 251/467 (53.75%), Postives = 329/467 (70.45%), Query Frame = 0

Query: 60  DGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 119
           DG  DVIIVGAGV GSALA+ L KDGRRVHVIERDL EP+RI+GE +QPGG L L +LGL
Sbjct: 43  DGATDVIIVGAGVGGSALAYALAKDGRRVHVIERDLREPERIMGEFMQPGGRLMLSKLGL 102

Query: 120 QDCVEEIDAQKVYGYALFKDGKSTRLSYPLE--NFHSDVSGRSFHNGRFIQRMREKAASL 179
           +DC+E IDAQK  G  ++KDGK    S+P++  NF  D S RSFHNGRF+QR+R+KA+SL
Sbjct: 103 EDCLEGIDAQKATGMTVYKDGKEAVASFPVDNNNFPFDPSARSFHNGRFVQRLRQKASSL 162

Query: 180 PNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVD 239
           PNVRLE+GTV SL+EEKG IKGV YK+  GEE TA APLT+VCDGC+SNLRRSL     +
Sbjct: 163 PNVRLEEGTVKSLIEEKGVIKGVTYKNSAGEETTALAPLTVVCDGCYSNLRRSLNDNNAE 222

Query: 240 VPSCFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEM 299
           V S  VG + +NCQL       +++  PS  + Y ISST++RC+ +V    +PSISNGEM
Sbjct: 223 VLSYQVGFISKNCQLEEPEKLKLIMSKPSFTMLYQISSTDVRCVFEVLPNNIPSISNGEM 282

Query: 300 EKYLKTVVAPQVPPEIHDAFIAAIEKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRHP 359
             ++K  +APQVP ++   F+  I++G +I+ MP + M A      G +L+GDAFNMRHP
Sbjct: 283 ATFVKNTIAPQVPLKLRKIFLKGIDEGEHIKAMPTKKMTATLSEKKGVILLGDAFNMRHP 342

Query: 360 LTGGGMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKV 419
               GM V LSDI++LR LL+PL NL +A  + + ++SFY +RKP+++T+NTL  A  +V
Sbjct: 343 AIASGMMVLLSDILILRRLLQPLSNLGNAQKISQVIKSFYDIRKPMSATVNTLGNAFSQV 402

Query: 420 FCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLL 479
             AS D+A++ MRQ C+DYLS GG  ++G ++LL G+NPRP+SL+ H  A+ +  +G LL
Sbjct: 403 LVASTDEAKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIGHLL 462

Query: 480 LPFPSLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRS 524
            PFPS   IW   RL   A  ++ P +KAEGV QM FP    AY +S
Sbjct: 463 SPFPSPLRIWHSLRLFGLAMKMLVPHLKAEGVSQMLFPVNAAAYSKS 509

BLAST of HG10005014 vs. TAIR 10
Match: AT5G24160.1 (squalene monoxygenase 6 )

HSP 1 Score: 494.2 bits (1271), Expect = 1.3e-139
Identity = 245/468 (52.35%), Postives = 329/468 (70.30%), Query Frame = 0

Query: 60  DGDADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 119
           DG ADVIIVGAGV GSALA+ L KDGRRVHVIERD+ EP+R++GE +QPGG L L +LGL
Sbjct: 43  DGAADVIIVGAGVGGSALAYALAKDGRRVHVIERDMREPERMMGEFMQPGGRLMLSKLGL 102

Query: 120 QDCVEEIDAQKVYGYALFKDGKSTRLSYPLE--NFHSDVSGRSFHNGRFIQRMREKAASL 179
           QDC+E+IDAQK  G A++KDGK     +P++  NF  + S RSFHNGRF+Q++R KA SL
Sbjct: 103 QDCLEDIDAQKATGLAVYKDGKEADAPFPVDNNNFSYEPSARSFHNGRFVQQLRRKAFSL 162

Query: 180 PNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKP-MV 239
            NVRLE+GTV SLLEEKG +KGV YK+K GEE TA APLT+VCDGC+SNLRRSL      
Sbjct: 163 SNVRLEEGTVKSLLEEKGVVKGVTYKNKEGEETTALAPLTVVCDGCYSNLRRSLNDDNNA 222

Query: 240 DVPSCFVGLVLENCQLPFANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGE 299
           ++ S  VG + +NC+L      H++L  PS  + Y ISST++RC  +V  +  PSI+NGE
Sbjct: 223 EIMSYIVGYISKNCRLEEPEKLHLILSKPSFTMVYQISSTDVRCGFEVLPENFPSIANGE 282

Query: 300 MEKYLKTVVAPQVPPEIHDAFIAAIEKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRH 359
           M  ++K  + PQVPP++   F+  I++G +I+ +P + M +      G +++GDAFNMRH
Sbjct: 283 MSTFMKNTIVPQVPPKLRKIFLKGIDEGAHIKVVPAKRMTSTLSKKKGVIVLGDAFNMRH 342

Query: 360 PLTGGGMTVALSDIVVLRNLLKPLKNLNDAPTLCKYLESFYTLRKPVASTINTLAGALYK 419
           P+   GM V LSDI++LR LL+PL NL DA  + + + SFY +RKP+++T+NTL  A  +
Sbjct: 343 PVVASGMMVLLSDILILRRLLQPLSNLGDANKVSEVINSFYDIRKPMSATVNTLGNAFSQ 402

Query: 420 VFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRL 479
           V   S D+A++ MRQ  +DYL  GG  ++G ++LL G+NPRPLSLV H  A+ +  +G+L
Sbjct: 403 VLIGSTDEAKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLSSIGQL 462

Query: 480 LLPFPSLKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRS 524
           L PFPS   IW   +L   A  ++ P +KAEGV QM FPA   AY++S
Sbjct: 463 LSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886247.11.6e-29395.66squalene monooxygenase SE1-like [Benincasa hispida][more]
XP_022996831.14.2e-28994.92squalene monooxygenase-like isoform X1 [Cucurbita maxima][more]
XP_008444517.17.1e-28995.44PREDICTED: squalene monooxygenase-like [Cucumis melo][more]
KAA0060974.17.1e-28995.44squalene monooxygenase-like [Cucumis melo var. makuwa][more]
KAG6598316.19.3e-28994.92Squalene monooxygenase SE2, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
Match NameE-valueIdentityDescription
B7TWW55.4e-23175.57Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2[more]
O486517.1e-23174.24Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1[more]
Q9SM024.3e-22875.29Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1[more]
Q8VYH21.5e-22574.29Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1[more]
O810004.1e-22375.64Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1K5U92.0e-28994.92Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491959 PE=3 SV=1[more]
A0A5A7V0X53.5e-28995.44Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5... [more]
A0A1S3BB883.5e-28995.44Squalene monooxygenase OS=Cucumis melo OX=3656 GN=LOC103487810 PE=3 SV=1[more]
A0A0A0LKI11.0e-28894.69Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G353480 PE=3 SV=1[more]
A0A6J1HEG75.0e-28894.73Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111462746 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G58440.13.0e-22975.29FAD/NAD(P)-binding oxidoreductase family protein [more]
AT4G37760.11.1e-22674.29squalene epoxidase 3 [more]
AT2G22830.12.9e-22475.64squalene epoxidase 2 [more]
AT5G24150.15.7e-14353.75FAD/NAD(P)-binding oxidoreductase family protein [more]
AT5G24160.11.3e-13952.35squalene monoxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00420RNGMNOXGNASEcoord: 64..86
score: 43.04
coord: 341..356
score: 30.83
coord: 213..228
score: 31.25
coord: 356..372
score: 32.55
NoneNo IPR availablePFAMPF13450NAD_binding_8coord: 67..93
e-value: 3.2E-5
score: 24.1
NoneNo IPR availablePANTHERPTHR10835:SF17SQUALENE MONOOXYGENASE-RELATEDcoord: 1..526
IPR013698Squalene epoxidasePFAMPF08491SEcoord: 214..483
e-value: 1.8E-119
score: 397.9
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 52..417
e-value: 1.5E-33
score: 118.2
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 63..438
IPR040125Squalene monooxygenasePANTHERPTHR10835SQUALENE MONOOXYGENASEcoord: 1..526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10005014.1HG10005014.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0004506 squalene monooxygenase activity