HG10004604 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10004604 vs. NCBI nr
Match: XP_038886465.1 (uncharacterized protein CG45076 [Benincasa hispida])

HSP 1 Score: 530.4 bits (1365), Expect = 1.2e-146
Identity = 307/368 (83.42%), Postives = 326/368 (88.59%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +P+T+SKSGPAPIPEGKPSLNRRRSFDKPP TAPRL KPFRSPGPRNRA HVPVRSSSFG
Sbjct: 94  KPVTRSKSGPAPIPEGKPSLNRRRSFDKPPSTAPRLGKPFRSPGPRNRAAHVPVRSSSFG 153

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPSTT     TIVNSTKPG L+RSSSK+SKVGGKPQQPI +LKSS SNVKKSLKRE SN
Sbjct: 154 AKPSTT-----TIVNSTKPGFLDRSSSKISKVGGKPQQPIHSLKSSNSNVKKSLKRESSN 213

Query: 131 A---ASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPD 190
           A   AS SASAS SASASAE++PKTKNA+EHVD SPSFVLGVNEEDLKKIECELDPYLPD
Sbjct: 214 AAASASVSASASASASASAEIVPKTKNAVEHVDHSPSFVLGVNEEDLKKIECELDPYLPD 273

Query: 191 PMPELDKQLRIDQVEK-----NETDDTENFKVVVTNKEVEEETPEAAQTEMSLMNREQNE 250
           PMPELDKQLRIDQVEK      +  DTE FKVVV NKEV EE PEAA+TE+SL NRE++E
Sbjct: 274 PMPELDKQLRIDQVEKKAVLDKDEADTETFKVVVANKEV-EEIPEAAETEISLTNRERDE 333

Query: 251 AEGDEEKTERITVEKEEVIASEE-NTNNKNDQEKSETDPILDGESYKEENDHKATETTEK 310
            EG+EEK ERI VEKEEV+ SEE NTNNKNDQEKSETDP+LDGESYKEENDHKA ETTEK
Sbjct: 334 VEGEEEKPERIAVEKEEVVGSEEINTNNKNDQEKSETDPVLDGESYKEENDHKAAETTEK 393

Query: 311 EGEATDKVAAAVAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTV 370
           E EAT+K AAAV  AKP+QGGP RKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTV
Sbjct: 394 EAEATEKAAAAV-VAKPRQGGPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTV 453

BLAST of HG10004604 vs. NCBI nr
Match: KAA0064998.1 (serine/arginine repetitive matrix protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 486.9 bits (1252), Expect = 1.6e-133
Identity = 291/373 (78.02%), Postives = 316/373 (84.72%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +PIT+SKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRA HVPVRSSSFG
Sbjct: 93  KPITRSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFG 152

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPST+TT    I +STKPG LERSSSK+SKVGGKPQ PIQ+LK+S+S+VKKSL+RE SN
Sbjct: 153 AKPSTSTT---IIGHSTKPGFLERSSSKISKVGGKPQPPIQSLKTSSSHVKKSLRRESSN 212

Query: 131 AASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 190
            A   ASAS SASASAE++PKTKNA+EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP
Sbjct: 213 VA---ASASASASASAEIVPKTKNAVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 272

Query: 191 ELDKQLRIDQVEK-----NETDDTENFKVVVTNKEVEE-ETPEAAQTEMSLMNREQNEAE 250
           ELDKQ+RIDQVEK      +  DTE  KVVVTNKEVEE ETP  A+TE+SL NREQNE E
Sbjct: 273 ELDKQVRIDQVEKKAALDKDETDTEILKVVVTNKEVEEKETPGTAETEISLANREQNEGE 332

Query: 251 GDEEKTE----RITVEKEEVIASEENTNNKNDQEKSETDPILDGESYKEENDH----KAT 310
            +E K E    RI VEKEEV+A EE+ NNKNDQEKSETD +LDGES KEEND     KA 
Sbjct: 333 EEEPKEEDKPKRIGVEKEEVLALEESINNKNDQEKSETDSVLDGESCKEENDQNSTPKAA 392

Query: 311 ETTEKEGEATDKVAAAVAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAG 370
           ETTEKE E+T+K       AKP+QG P RKESPA+YNHVIEETASKLLEKRKNKVRALAG
Sbjct: 393 ETTEKEAESTEK------TAKPRQGAPGRKESPAVYNHVIEETASKLLEKRKNKVRALAG 452

BLAST of HG10004604 vs. NCBI nr
Match: XP_011649760.1 (neurofilament heavy polypeptide [Cucumis sativus] >KGN62866.1 hypothetical protein Csa_021828 [Cucumis sativus])

HSP 1 Score: 484.2 bits (1245), Expect = 1.0e-132
Identity = 290/377 (76.92%), Postives = 316/377 (83.82%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +PI +SKSGPAPIPEG+PSLNRRRSFDKPPPT PRL KPFRSPGPRNR  HVPVRSSSFG
Sbjct: 95  KPIARSKSGPAPIPEGRPSLNRRRSFDKPPPTVPRLGKPFRSPGPRNRTPHVPVRSSSFG 154

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPSTTTT   TI +STKPG LERSSSK SKVGGKPQ PIQ+LK+S+S VKKSL+RE SN
Sbjct: 155 AKPSTTTT---TIGHSTKPGFLERSSSKSSKVGGKPQPPIQSLKTSSSYVKKSLRRESSN 214

Query: 131 A---ASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPD 190
           A   ASASASAS SASASAE++PK+KNA+EHVDQSPSFVLGVNEEDLKKIECELDPYLPD
Sbjct: 215 AAASASASASASASASASAEIVPKSKNAVEHVDQSPSFVLGVNEEDLKKIECELDPYLPD 274

Query: 191 PMPELDKQLRIDQVEK-----NETDDTENFKVVVTNKEVEE--ETPEAAQTEMSLMNREQ 250
           PMPELDKQ+R+DQVEK      +  DTE  KVVVTNKEVEE  ETP   +TE+SL N+EQ
Sbjct: 275 PMPELDKQIRLDQVEKKAVLDKDETDTEILKVVVTNKEVEEQKETPRTVETEISLANQEQ 334

Query: 251 NEAEGDEEKTE----RITVEKEEVIASEENTNNKNDQEKSETDPILDGESYKEENDH--- 310
           NE EG+E K E    RI VEKEEV+A EE+ NNKNDQEKSETD +LDGES KEE+D    
Sbjct: 335 NEREGEEPKEEDNPKRIEVEKEEVVALEESINNKNDQEKSETDSVLDGESCKEESDQNST 394

Query: 311 -KATETTEKEGEATDKVAAAVAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVR 370
            KA ETTEKE E+T+K       AKP+QGGP RKESPAMYNHVIEETASKLLEKRKNKVR
Sbjct: 395 PKAAETTEKEAESTEK------TAKPRQGGPGRKESPAMYNHVIEETASKLLEKRKNKVR 454

BLAST of HG10004604 vs. NCBI nr
Match: XP_008445084.1 (PREDICTED: serine/arginine repetitive matrix protein 1 [Cucumis melo])

HSP 1 Score: 483.8 bits (1244), Expect = 1.3e-132
Identity = 289/373 (77.48%), Postives = 314/373 (84.18%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +PIT+SKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRA HVPVRSSSFG
Sbjct: 93  KPITRSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFG 152

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPST+TT    I +STKPG LERSSSK+SKVGGKPQ PIQ+LK+S+S+VKKSL+RE SN
Sbjct: 153 AKPSTSTT---IIGHSTKPGFLERSSSKISKVGGKPQPPIQSLKTSSSHVKKSLRRESSN 212

Query: 131 AASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 190
            A     AS SASASAE++PKTKNA+EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP
Sbjct: 213 VA-----ASASASASAEIVPKTKNAVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 272

Query: 191 ELDKQLRIDQVEK-----NETDDTENFKVVVTNKEVEE-ETPEAAQTEMSLMNREQNEAE 250
           ELDKQ+RIDQVEK      +  DTE  KVVVTNKEVEE ETP  A+TE+SL NREQNE E
Sbjct: 273 ELDKQVRIDQVEKKAALDKDETDTEILKVVVTNKEVEEKETPGTAETEISLANREQNEGE 332

Query: 251 GDEEKTE----RITVEKEEVIASEENTNNKNDQEKSETDPILDGESYKEENDH----KAT 310
            +E K E    RI VEKEEV+A EE+ NNKNDQEKSETD +LDGES KEEND     KA 
Sbjct: 333 EEEPKEEDKPKRIGVEKEEVLALEESINNKNDQEKSETDSVLDGESCKEENDQNSTPKAA 392

Query: 311 ETTEKEGEATDKVAAAVAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAG 370
           ETTEKE E+T+K       AKP+QG P RKESPA+YNHVIEETASKLLEKRKNKVRALAG
Sbjct: 393 ETTEKEAESTEK------TAKPRQGAPGRKESPAVYNHVIEETASKLLEKRKNKVRALAG 451

BLAST of HG10004604 vs. NCBI nr
Match: XP_023546370.1 (cell wall protein RBR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 395.2 bits (1014), Expect = 6.2e-106
Identity = 265/398 (66.58%), Postives = 291/398 (73.12%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +PI KS +GP PIPEGKPSLNRRRSFDKPPP   RL KPFRSP PRNRA H PVRSSSFG
Sbjct: 93  KPIKKS-AGPTPIPEGKPSLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFG 152

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPST      TIV+STK G LERS SK+ KVGGK QQ +Q LKSS SNV+KSLKRE SN
Sbjct: 153 AKPST------TIVSSTKTGFLERSGSKIYKVGGKTQQSVQTLKSS-SNVRKSLKRESSN 212

Query: 131 AASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 190
           AASAS         SAEM+PKTKNA+EHV QSP  VLG NEEDLKKIECELD  LPDP P
Sbjct: 213 AASAS---------SAEMVPKTKNAVEHVGQSPVLVLGANEEDLKKIECELDLCLPDPRP 272

Query: 191 ELDKQL-RIDQVEK-----NETDDTENFKVVVTNKEVEEETPEAAQTEMSLM------NR 250
           ELDK+L   DQVEK         DT+ FKVV +NK+V EETP AA+T+ +        NR
Sbjct: 273 ELDKELSTTDQVEKRLHHHQPIQDTKIFKVVGSNKKV-EETPGAAETDNAKSSIREDENR 332

Query: 251 EQNEAEG-----DEEKTERITVEKEE---VIASEENTNNKNDQEKSETDPIL-------- 310
           EQNE EG     +EEK ERI VEKEE   V+ASEE+TNNKN++EKS TDPI         
Sbjct: 333 EQNEGEGEEEAKEEEKPERIEVEKEEVFGVVASEESTNNKNEEEKSHTDPIQVGVIVLEH 392

Query: 311 ----DGESYKEENDHKATETTEKEGEATDKVAAA-------VAAAKPQQGGPARKESPAM 370
               DG+S KEEN +    +TEKE EAT+KVAAA        AAAKP+QGGP RKESPA+
Sbjct: 393 KEVEDGQSSKEENHN----STEKEAEATEKVAAAAAAAAAEAAAAKPRQGGPGRKESPAV 452

BLAST of HG10004604 vs. ExPASy TrEMBL
Match: A0A5A7VBK4 (Serine/arginine repetitive matrix protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003220 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 7.6e-134
Identity = 291/373 (78.02%), Postives = 316/373 (84.72%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +PIT+SKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRA HVPVRSSSFG
Sbjct: 93  KPITRSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFG 152

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPST+TT    I +STKPG LERSSSK+SKVGGKPQ PIQ+LK+S+S+VKKSL+RE SN
Sbjct: 153 AKPSTSTT---IIGHSTKPGFLERSSSKISKVGGKPQPPIQSLKTSSSHVKKSLRRESSN 212

Query: 131 AASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 190
            A   ASAS SASASAE++PKTKNA+EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP
Sbjct: 213 VA---ASASASASASAEIVPKTKNAVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 272

Query: 191 ELDKQLRIDQVEK-----NETDDTENFKVVVTNKEVEE-ETPEAAQTEMSLMNREQNEAE 250
           ELDKQ+RIDQVEK      +  DTE  KVVVTNKEVEE ETP  A+TE+SL NREQNE E
Sbjct: 273 ELDKQVRIDQVEKKAALDKDETDTEILKVVVTNKEVEEKETPGTAETEISLANREQNEGE 332

Query: 251 GDEEKTE----RITVEKEEVIASEENTNNKNDQEKSETDPILDGESYKEENDH----KAT 310
            +E K E    RI VEKEEV+A EE+ NNKNDQEKSETD +LDGES KEEND     KA 
Sbjct: 333 EEEPKEEDKPKRIGVEKEEVLALEESINNKNDQEKSETDSVLDGESCKEENDQNSTPKAA 392

Query: 311 ETTEKEGEATDKVAAAVAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAG 370
           ETTEKE E+T+K       AKP+QG P RKESPA+YNHVIEETASKLLEKRKNKVRALAG
Sbjct: 393 ETTEKEAESTEK------TAKPRQGAPGRKESPAVYNHVIEETASKLLEKRKNKVRALAG 452

BLAST of HG10004604 vs. ExPASy TrEMBL
Match: A0A0A0LS85 (CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G378000 PE=4 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.9e-133
Identity = 290/377 (76.92%), Postives = 316/377 (83.82%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +PI +SKSGPAPIPEG+PSLNRRRSFDKPPPT PRL KPFRSPGPRNR  HVPVRSSSFG
Sbjct: 95  KPIARSKSGPAPIPEGRPSLNRRRSFDKPPPTVPRLGKPFRSPGPRNRTPHVPVRSSSFG 154

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPSTTTT   TI +STKPG LERSSSK SKVGGKPQ PIQ+LK+S+S VKKSL+RE SN
Sbjct: 155 AKPSTTTT---TIGHSTKPGFLERSSSKSSKVGGKPQPPIQSLKTSSSYVKKSLRRESSN 214

Query: 131 A---ASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPD 190
           A   ASASASAS SASASAE++PK+KNA+EHVDQSPSFVLGVNEEDLKKIECELDPYLPD
Sbjct: 215 AAASASASASASASASASAEIVPKSKNAVEHVDQSPSFVLGVNEEDLKKIECELDPYLPD 274

Query: 191 PMPELDKQLRIDQVEK-----NETDDTENFKVVVTNKEVEE--ETPEAAQTEMSLMNREQ 250
           PMPELDKQ+R+DQVEK      +  DTE  KVVVTNKEVEE  ETP   +TE+SL N+EQ
Sbjct: 275 PMPELDKQIRLDQVEKKAVLDKDETDTEILKVVVTNKEVEEQKETPRTVETEISLANQEQ 334

Query: 251 NEAEGDEEKTE----RITVEKEEVIASEENTNNKNDQEKSETDPILDGESYKEENDH--- 310
           NE EG+E K E    RI VEKEEV+A EE+ NNKNDQEKSETD +LDGES KEE+D    
Sbjct: 335 NEREGEEPKEEDNPKRIEVEKEEVVALEESINNKNDQEKSETDSVLDGESCKEESDQNST 394

Query: 311 -KATETTEKEGEATDKVAAAVAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVR 370
            KA ETTEKE E+T+K       AKP+QGGP RKESPAMYNHVIEETASKLLEKRKNKVR
Sbjct: 395 PKAAETTEKEAESTEK------TAKPRQGGPGRKESPAMYNHVIEETASKLLEKRKNKVR 454

BLAST of HG10004604 vs. ExPASy TrEMBL
Match: A0A1S3BBU4 (serine/arginine repetitive matrix protein 1 OS=Cucumis melo OX=3656 GN=LOC103488229 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 6.4e-133
Identity = 289/373 (77.48%), Postives = 314/373 (84.18%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +PIT+SKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRA HVPVRSSSFG
Sbjct: 93  KPITRSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFG 152

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPST+TT    I +STKPG LERSSSK+SKVGGKPQ PIQ+LK+S+S+VKKSL+RE SN
Sbjct: 153 AKPSTSTT---IIGHSTKPGFLERSSSKISKVGGKPQPPIQSLKTSSSHVKKSLRRESSN 212

Query: 131 AASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 190
            A     AS SASASAE++PKTKNA+EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP
Sbjct: 213 VA-----ASASASASAEIVPKTKNAVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 272

Query: 191 ELDKQLRIDQVEK-----NETDDTENFKVVVTNKEVEE-ETPEAAQTEMSLMNREQNEAE 250
           ELDKQ+RIDQVEK      +  DTE  KVVVTNKEVEE ETP  A+TE+SL NREQNE E
Sbjct: 273 ELDKQVRIDQVEKKAALDKDETDTEILKVVVTNKEVEEKETPGTAETEISLANREQNEGE 332

Query: 251 GDEEKTE----RITVEKEEVIASEENTNNKNDQEKSETDPILDGESYKEENDH----KAT 310
            +E K E    RI VEKEEV+A EE+ NNKNDQEKSETD +LDGES KEEND     KA 
Sbjct: 333 EEEPKEEDKPKRIGVEKEEVLALEESINNKNDQEKSETDSVLDGESCKEENDQNSTPKAA 392

Query: 311 ETTEKEGEATDKVAAAVAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAG 370
           ETTEKE E+T+K       AKP+QG P RKESPA+YNHVIEETASKLLEKRKNKVRALAG
Sbjct: 393 ETTEKEAESTEK------TAKPRQGAPGRKESPAVYNHVIEETASKLLEKRKNKVRALAG 451

BLAST of HG10004604 vs. ExPASy TrEMBL
Match: A0A6J1K599 (microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492415 PE=4 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 6.7e-106
Identity = 263/395 (66.58%), Postives = 292/395 (73.92%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +PI KS +GPAPIPEGKPSLNRRRSFDKPPP   RL KPFRSP PRNRA H PVRSSSFG
Sbjct: 92  KPIKKS-AGPAPIPEGKPSLNRRRSFDKPPPAMQRLGKPFRSPEPRNRAAHAPVRSSSFG 151

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPST      T+V+STK G LERS SK+ KVGGK QQ +Q  KSS SN++KSLKRE SN
Sbjct: 152 AKPST------TLVSSTKTGFLERSGSKIYKVGGKTQQSVQTFKSS-SNLRKSLKRESSN 211

Query: 131 AASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 190
           AASAS         SAEM+PKTKNA+EHV QSP  VLGVNEEDLKKIECELD  LPDP P
Sbjct: 212 AASAS---------SAEMVPKTKNAVEHVGQSPVLVLGVNEEDLKKIECELDLCLPDPRP 271

Query: 191 ELDKQL-RIDQVEK-----NETDDTENFKVVVTNKEVEEETPEAAQTEMSLM------NR 250
           ELDK+L   DQVEK         DT+ FKVV +NK+V EETP AA+T+ +        N+
Sbjct: 272 ELDKELSTTDQVEKRLHHHQPIQDTKIFKVVGSNKKV-EETPGAAETDNAKSSIWEDENQ 331

Query: 251 EQNEAEG-----DEEKTERITVEKEE---VIASEENTNNKNDQEKSETDPIL-------- 310
           EQNE EG     +EEK ERI VEKEE   V+ASEE+TNNKN++EKS TDPI         
Sbjct: 332 EQNEGEGEEEAKEEEKPERIEVEKEEVYGVVASEESTNNKNEEEKSHTDPIQEGVIVLEH 391

Query: 311 ----DGESYKEENDHKATETTEKEGEATDKVAAAVA----AAKPQQGGPARKESPAMYNH 370
               DG+S KEEN +    +TEKE EAT+KVAAA A    AAKP+QGGP RKESPA+YNH
Sbjct: 392 KEAEDGQSSKEENHN----STEKEAEATEKVAAAAAAAAEAAKPRQGGPGRKESPAVYNH 451

BLAST of HG10004604 vs. ExPASy TrEMBL
Match: A0A6J1HE16 (neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462632 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.4e-103
Identity = 262/397 (65.99%), Postives = 288/397 (72.54%), Query Frame = 0

Query: 11  RPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFG 70
           +PI KS  GPAPIPEGKPSLNRRRSFDKPPP   RL KPFRSP PRNRA H PVRSSSFG
Sbjct: 93  KPIKKS-GGPAPIPEGKPSLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFG 152

Query: 71  AKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSN 130
           AKPST      +IV+STK G LERS SK+ KVGGK QQ +Q LKSS SNV+KSLKRE SN
Sbjct: 153 AKPST------SIVSSTKTGFLERSGSKIYKVGGKTQQSVQTLKSS-SNVRKSLKRESSN 212

Query: 131 AASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMP 190
           AASAS         SAEM+PKTKNA+EHV QSP  VLG NEEDLKKIECELD  LPDP P
Sbjct: 213 AASAS---------SAEMVPKTKNAVEHVGQSPVLVLGANEEDLKKIECELDLCLPDPRP 272

Query: 191 ELDKQL-RIDQVEK-----NETDDTENFKVVVTNKEVEEETPEAAQTEMSLM------NR 250
           ELDK+L   DQVEK         +T+ FKVV +NK+V EETP AA+T+ +        NR
Sbjct: 273 ELDKELSTTDQVEKRLHHHQPIQETKIFKVVGSNKKV-EETPGAAETDNAKSSIREDENR 332

Query: 251 EQNEAEG-----DEEKTERITVEKEE---VIASEENTNNKNDQEKSETDPIL-------- 310
           EQNE EG     +EEK ERI VEKEE   V+ASEE+TNNKN++EKS T PI         
Sbjct: 333 EQNEGEGEEEAKEEEKPERIEVEKEEVFGVVASEESTNNKNEEEKSHTVPIQEGVIVLEH 392

Query: 311 ----DGESYKEENDHKATETTEKEGEATDKVAAA------VAAAKPQQGGPARKESPAMY 370
               DG+S KEEN      +TEKE EAT+KVAAA        AAKP+QGGP RKESPA+Y
Sbjct: 393 KEAEDGQSSKEENH----TSTEKETEATEKVAAAATSAAEAVAAKPRQGGPGRKESPAVY 452

BLAST of HG10004604 vs. TAIR 10
Match: AT5G24880.1 (BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (TAIR:AT5G10660.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 53.9 bits (128), Expect = 3.1e-07
Identity = 111/386 (28.76%), Postives = 168/386 (43.52%), Query Frame = 0

Query: 30  LNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKP 89
           L RRRSFD+PP +   L  P  S  PR +         S    PS   +     V   KP
Sbjct: 80  LLRRRSFDRPPSS---LTSPSTSASPRIQ--------KSLNVSPS--RSRDRPAVPREKP 139

Query: 90  GLLERSSS-----KVSKVGGKPQQPIQALK----SSTSNVKKSLKREPSNAASASASASV 149
               RSSS      V K G   + P  A K    +S+S   KS K    N     AS   
Sbjct: 140 VTALRSSSFHGSRNVPKGGNTAKSPPVAPKKSGLNSSSTSSKSKKEGSENVRIKKASDKE 199

Query: 150 SASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQ 209
            A  SA M    ++  E + +  S  L V++ D+++ + E +        E + Q ++ Q
Sbjct: 200 IALDSASMSSAQEDHQEEILKVESDHLQVSDHDIEEPKYEKE--------EKEVQEKVVQ 259

Query: 210 VEKNETDDTENF----------------------KVVVTNKEVEEETPEAAQTEMSLMNR 269
             ++  +  E+                       K++    ++EE+T E  + + +  N+
Sbjct: 260 ANESVEEKAESSGPTPVASPVGKDCNAVVAELEEKLIKNEDDIEEKTEEMKEQDNNQANK 319

Query: 270 EQNEAE-----GDEEKTERITVEKEEVIASEENTNNKNDQEKSE-----TDPILDGESYK 329
            + E +      + E  E++  E +EV + EE T  K ++ K E      +   + E  K
Sbjct: 320 SEEEEDVKKKIDENETPEKVDTESKEVESVEETTQEKEEEVKEEGKERVEEEEKEKEKVK 379

Query: 330 EENDHKATETTEKEGEATDKVAAAV-----AAAKPQQGGPARKESPAMYNHVIEETASKL 369
           E++  +  E  EKE    D+    V     A  K ++    +KESP+ YN VI   ASK+
Sbjct: 380 EDDQKEKVEEEEKEKVKGDEEKEKVKEEESAEGKKKEVVKGKKESPSAYNDVI---ASKM 439

BLAST of HG10004604 vs. TAIR 10
Match: AT5G10660.1 (calmodulin-binding protein-related )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 110/366 (30.05%), Postives = 161/366 (43.99%), Query Frame = 0

Query: 25  EGKPSLNRRRSFDKPPP--TAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNT 84
           E    L RRRSFD PP   T+P      RS        H+  + +    KP T   +T +
Sbjct: 76  EENQKLLRRRSFDHPPSSLTSPSTSSAHRSLNTSPAHPHLRDKPAVPREKPVTGLRST-S 135

Query: 85  IVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNAASASASASVSA 144
              S++ GL  R SS V       + P  A + S+   K  L    S+ +    S +V  
Sbjct: 136 FHGSSRGGL--RGSSTV-------KSPPVASRGSSGVKKSGLSGNSSSKSKKEGSGNVPK 195

Query: 145 SASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVE 204
            +S + I          D SP      +EE++ K+E ++                 D  E
Sbjct: 196 KSSGKEISP--------DSSPLASAHEDEEEIVKVETDVH--------------ISDHGE 255

Query: 205 KNETDDTENFKVVVTNKEVEEETPEAAQTE--MSLMNREQNEAEGDEEKTERITVEKEEV 264
           + + +D + F     + E +E +P AA TE     +  E    E  EE  E   +E+   
Sbjct: 256 EPKEEDKDQFAQPDESGEEKETSPVAASTEEQKGELIDEDKSTEQIEEPKEPENIEENNS 315

Query: 265 IASEENTNNKNDQEKSET-------DPILDGESYKEENDHKATETTEKEGE---ATDKVA 324
              EE     +D+E SET       +  ++ E  KEE   +A E  E+EGE   A ++  
Sbjct: 316 EEEEEVKKKSDDEENSETVATTTDMNEAVNVEESKEEEKEEA-EVKEEEGESSAAKEETT 375

Query: 325 AAVAAAK--PQQGG-----PARKESPAMYNHVIEETASKLLE-KRKNKVRALAGAFQTVI 369
             +A  +  P++G        +KESP  YN VI   ASK+ E  +KNKV ALAGAFQTVI
Sbjct: 376 ETMAQVEELPEEGTKNEVVQGKKESPTAYNDVI---ASKMQENSKKNKVLALAGAFQTVI 405