HG10004481 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10004481 vs. NCBI nr
Match: XP_038885360.1 (dihydrofolate reductase-like [Benincasa hispida])

HSP 1 Score: 440.7 bits (1132), Expect = 1.2e-119
Identity = 214/238 (89.92%), Postives = 221/238 (92.86%), Query Frame = 0

Query: 111 LAAALPGLQA-KRIPIMGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLD 170
           + +  P L    RIPIMGTD K  RKPRFLCLHGFRTSAAILKKQVGKWPASVLDQ DL 
Sbjct: 1   MTSPFPSLHTNSRIPIMGTDAKIHRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQFDLH 60

Query: 171 FLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLG 230
           FLDAPFPAEGKSDVEGIFDPPYFEWFQFS EFTEYRNFDECLSFIEDYMLKHGPFDGFLG
Sbjct: 61  FLDAPFPAEGKSDVEGIFDPPYFEWFQFSQEFTEYRNFDECLSFIEDYMLKHGPFDGFLG 120

Query: 231 FSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFL 290
           FSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRS+SVA+ AYST IGCPSLHFL
Sbjct: 121 FSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAQKAYSTPIGCPSLHFL 180

Query: 291 GEEDFLMPHGQKLLESYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           GEEDFLMPHGQKLLESYVEPT+INHPKGHT+PRLDDK LEVMESFI+RISKTLDETEE
Sbjct: 181 GEEDFLMPHGQKLLESYVEPTVINHPKGHTVPRLDDKGLEVMESFIKRISKTLDETEE 238

BLAST of HG10004481 vs. NCBI nr
Match: XP_023545459.1 (dihydrofolate reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 436.8 bits (1122), Expect = 1.7e-118
Identity = 208/232 (89.66%), Postives = 218/232 (93.97%), Query Frame = 0

Query: 116 PGLQAKRIPIMGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPF 175
           P    +RIPIMGTDG+  RKPRFLCLHGFRTS AILKKQVGKWP SVL+QLDLDFLDAPF
Sbjct: 18  PEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF 77

Query: 176 PAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 235
           PAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI
Sbjct: 78  PAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 137

Query: 236 LSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDFL 295
           LSAALPG QAKGIALTKVPKIKFVIIVSGAKFRSQSVAE AYS+AIGCPSLHFLG+EDFL
Sbjct: 138 LSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFL 197

Query: 296 MPHGQKLLESYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           MPHGQKLLESY+EP +INHPKGHTIPRLDDK LEVMESFI++ISK LDE EE
Sbjct: 198 MPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 249

BLAST of HG10004481 vs. NCBI nr
Match: XP_022997250.1 (dihydrofolate reductase-like [Cucurbita maxima])

HSP 1 Score: 434.5 bits (1116), Expect = 8.7e-118
Identity = 206/232 (88.79%), Postives = 218/232 (93.97%), Query Frame = 0

Query: 116 PGLQAKRIPIMGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPF 175
           P    +RIPIMGTDG+  RKPRFLCLHGFRTS AILKKQVGKWP SVL+QLDLDFLDAPF
Sbjct: 18  PEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF 77

Query: 176 PAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 235
           PAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI
Sbjct: 78  PAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 137

Query: 236 LSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDFL 295
           LSAALPG QAKG+ALTKVPKIKFVIIVSGAKFRSQSVAE AYS+AIGCPSLHFLG+EDFL
Sbjct: 138 LSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFL 197

Query: 296 MPHGQKLLESYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           MPHGQKLLESY+EP +INHPKGHTIPRLDD+ LEVMESFI++ISK LDE EE
Sbjct: 198 MPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVMESFIEKISKMLDEAEE 249

BLAST of HG10004481 vs. NCBI nr
Match: XP_022961839.1 (dihydrofolate reductase-like [Cucurbita moschata])

HSP 1 Score: 433.3 bits (1113), Expect = 1.9e-117
Identity = 207/232 (89.22%), Postives = 216/232 (93.10%), Query Frame = 0

Query: 116 PGLQAKRIPIMGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPF 175
           P    +RIPIMGTDG+  RKPRFLCLHGFRTS AILKKQVGKWP SVL+QLDLDFLDAPF
Sbjct: 18  PEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF 77

Query: 176 PAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 235
           PAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI
Sbjct: 78  PAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 137

Query: 236 LSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDFL 295
           LSAALPG QAKGIALTKVPKIKFVIIVSGAKFRSQSVAE AYS  IGCPSLHFLG+EDFL
Sbjct: 138 LSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLGKEDFL 197

Query: 296 MPHGQKLLESYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           MPHGQKLLESY+EP IINHPKGHTIPRLDD+ LEVMESFI++ISK LDE EE
Sbjct: 198 MPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVMESFIEKISKMLDEAEE 249

BLAST of HG10004481 vs. NCBI nr
Match: XP_004152614.1 (dihydrofolate reductase [Cucumis sativus])

HSP 1 Score: 431.4 bits (1108), Expect = 7.3e-117
Identity = 208/233 (89.27%), Postives = 217/233 (93.13%), Query Frame = 0

Query: 115 LPGLQAKRIPIMGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAP 174
           +P  +   IPIMG+D KF  KPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDL FLDAP
Sbjct: 17  IPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAP 76

Query: 175 FPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGA 234
           FPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM+KHGPFDGFLGFSQGA
Sbjct: 77  FPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGA 136

Query: 235 ILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDF 294
           ILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRS+SVAE AYST IGCPSLHFLGEEDF
Sbjct: 137 ILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDF 196

Query: 295 LMPHGQKLLESYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           LMPHG+KLLESY+EPTII HPKGHTIPRLDDKALEVMESFI RISK L+E EE
Sbjct: 197 LMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 249

BLAST of HG10004481 vs. ExPASy Swiss-Prot
Match: Q3SZ07 (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 7.1e-14
Identity = 61/204 (29.90%), Postives = 86/204 (42.16%), Query Frame = 0

Query: 137 RFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPFPAEGKSDVEGI---------F 196
           R LCL GFR S    +++ G    ++  + +L  L  P P    +  EG           
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 197 DPPYFEWFQFS--------PEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAA 256
           + P   WF            E T  R  +E L  +   + K GPFDG LGFSQGA L+A 
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 257 LPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDFLMP-- 316
           +      G    + P  +FVI+VSG   R   + E      +  PSLH  G+ D ++P  
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGLMEPIMQGPLSLPSLHVFGDTDGVIPSQ 187

Query: 317 HGQKLLESYVEPTIINHPKGHTIP 322
              +L   +     + H  GH IP
Sbjct: 188 ESMQLCSRFDGAVTLTHSGGHFIP 209

BLAST of HG10004481 vs. ExPASy Swiss-Prot
Match: Q8WZ82 (Esterase OVCA2 OS=Homo sapiens OX=9606 GN=OVCA2 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.8e-13
Identity = 60/207 (28.99%), Postives = 88/207 (42.51%), Query Frame = 0

Query: 134 RKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPFPAEGKSDVEGI------- 193
           R  R LCL GFR S    +++ G    ++  + +L  L  P P       EG        
Sbjct: 5   RPLRVLCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVPDPPGPEGARSDFGSC 64

Query: 194 --FDPPYFEWFQ------FS--PEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAIL 253
              + P   WF       FS   E    R  +E L  +   + + GPFDG LGFSQGA L
Sbjct: 65  PPEEQPRGWWFSEQEADVFSALEEPAVCRGLEESLGMVAQALNRLGPFDGLLGFSQGAAL 124

Query: 254 SAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDFLM 313
           +A +      G    + P  +F+++VSG   R     E+     +  PSLH  G+ D ++
Sbjct: 125 AALVCALGQAGD--PRFPLPRFILLVSGFCPRGIGFKESILQRPLSLPSLHVFGDTDKVI 184

Query: 314 PHGQ--KLLESYVEPTIINHPKGHTIP 322
           P  +  +L   +     + H  GH IP
Sbjct: 185 PSQESVQLASQFPGAITLTHSGGHFIP 209

BLAST of HG10004481 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.5e-11
Identity = 49/140 (35.00%), Postives = 68/140 (48.57%), Query Frame = 0

Query: 8   QRKPKILCLHGFRTSGAILKKQIGKWPNSVLNQFDFHFIDAP----FPSQGKSDVQGIYD 67
           +RK +ILCLHGFR + +  K + G     + N  +  FIDAP    F  Q  +   G+ +
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 68  PPYFEW-----FQHSKET---------------TGYENFESCLEFIENYMVEYGPFDGLL 124
              F W     F    ET               T  E F+  L +++    E GPFDG+L
Sbjct: 411 KK-FAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGIL 470

BLAST of HG10004481 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-11
Identity = 39/116 (33.62%), Postives = 57/116 (49.14%), Query Frame = 0

Query: 12  KILCLHGFRTSGAILKKQIGKWPNSVLNQFDFHFIDAPFPSQGKSD------------VQ 71
           K+LCLHG+  SG +  K++G     +    + HF   P  +  ++D            + 
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALG 65

Query: 72  GIYDPPYFEWFQHSKETTGYENFESCLEFIENYMVEYGPFDGLLGFSQGAALAAAL 116
           G  +   F WF+       Y +++  LE I  YM E GPFDGL+GFSQGA + A L
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAML 121

BLAST of HG10004481 vs. ExPASy Swiss-Prot
Match: S0EES1 (Esterase FUS5 OS=Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) OX=1279085 GN=FUS5 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.8e-10
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 0

Query: 10  KPKILCLHGFRTSGAILKKQIGKWPNSVLNQFDFHFIDAPFPSQGKSDVQGIYDP--PYF 69
           +P++LCLHG   S  I++ Q  K  +++   F   F++ P  S     V   ++   PY 
Sbjct: 4   RPRLLCLHGGGASSQIMRVQFSKLESALRKTFQLVFLEGPLDSAPGPGVLPFFEDFGPYS 63

Query: 70  EWFQHSKETTGYENFE---SCLEFIENYMVEYGPFDGLLGFSQGAALAAAL 116
            W    K     E  +   + + +I+ +M++YGPF G+LGFSQGA   A++
Sbjct: 64  CWVSDDKSLLPEEKRKEENNAIAYIKTFMLQYGPFAGILGFSQGARATASI 114

BLAST of HG10004481 vs. ExPASy TrEMBL
Match: A0A6J1K928 (dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 4.2e-118
Identity = 206/232 (88.79%), Postives = 218/232 (93.97%), Query Frame = 0

Query: 116 PGLQAKRIPIMGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPF 175
           P    +RIPIMGTDG+  RKPRFLCLHGFRTS AILKKQVGKWP SVL+QLDLDFLDAPF
Sbjct: 18  PEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF 77

Query: 176 PAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 235
           PAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI
Sbjct: 78  PAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 137

Query: 236 LSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDFL 295
           LSAALPG QAKG+ALTKVPKIKFVIIVSGAKFRSQSVAE AYS+AIGCPSLHFLG+EDFL
Sbjct: 138 LSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFL 197

Query: 296 MPHGQKLLESYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           MPHGQKLLESY+EP +INHPKGHTIPRLDD+ LEVMESFI++ISK LDE EE
Sbjct: 198 MPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVMESFIEKISKMLDEAEE 249

BLAST of HG10004481 vs. ExPASy TrEMBL
Match: A0A6J1HF66 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 9.3e-118
Identity = 207/232 (89.22%), Postives = 216/232 (93.10%), Query Frame = 0

Query: 116 PGLQAKRIPIMGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPF 175
           P    +RIPIMGTDG+  RKPRFLCLHGFRTS AILKKQVGKWP SVL+QLDLDFLDAPF
Sbjct: 18  PEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF 77

Query: 176 PAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 235
           PAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI
Sbjct: 78  PAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 137

Query: 236 LSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDFL 295
           LSAALPG QAKGIALTKVPKIKFVIIVSGAKFRSQSVAE AYS  IGCPSLHFLG+EDFL
Sbjct: 138 LSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLGKEDFL 197

Query: 296 MPHGQKLLESYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           MPHGQKLLESY+EP IINHPKGHTIPRLDD+ LEVMESFI++ISK LDE EE
Sbjct: 198 MPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVMESFIEKISKMLDEAEE 249

BLAST of HG10004481 vs. ExPASy TrEMBL
Match: A0A0A0LNX4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.5e-117
Identity = 208/233 (89.27%), Postives = 217/233 (93.13%), Query Frame = 0

Query: 115 LPGLQAKRIPIMGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAP 174
           +P  +   IPIMG+D KF  KPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDL FLDAP
Sbjct: 17  IPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAP 76

Query: 175 FPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGA 234
           FPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM+KHGPFDGFLGFSQGA
Sbjct: 77  FPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGA 136

Query: 235 ILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDF 294
           ILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRS+SVAE AYST IGCPSLHFLGEEDF
Sbjct: 137 ILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDF 196

Query: 295 LMPHGQKLLESYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           LMPHG+KLLESY+EPTII HPKGHTIPRLDDKALEVMESFI RISK L+E EE
Sbjct: 197 LMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 249

BLAST of HG10004481 vs. ExPASy TrEMBL
Match: A0A1S3BC97 (dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 4.6e-117
Identity = 207/235 (88.09%), Postives = 218/235 (92.77%), Query Frame = 0

Query: 113 AALPGLQAKRIPIMGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLD 172
           + +P      +PIMG+D K   KPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDL FLD
Sbjct: 15  STIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLD 74

Query: 173 APFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ 232
           APFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM++HGPFDGFLGFSQ
Sbjct: 75  APFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQ 134

Query: 233 GAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEE 292
           GAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRS+SVAE AYST IGCPSLHFLGEE
Sbjct: 135 GAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEE 194

Query: 293 DFLMPHGQKLLESYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           DFLMPHG+KLLESY+EPTII HPKGHTIPRLDDKALEVMESFIQRISK L+ETEE
Sbjct: 195 DFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 249

BLAST of HG10004481 vs. ExPASy TrEMBL
Match: A0A5A7VFL2 (Dihydrofolate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004510 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 5.7e-115
Identity = 204/222 (91.89%), Postives = 212/222 (95.50%), Query Frame = 0

Query: 126 MGTDGKFLRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPFPAEGKSDVEG 185
           MG+D K   KPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDL FLDAPFPAEGKSDVEG
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 186 IFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQA 245
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM++HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 246 KGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDFLMPHGQKLLES 305
           KGIALTKVPKIKFVIIVSGAKFRS+SVAE AYST IGCPSLHFLGEEDFLMPHG+KLLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 306 YVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           Y+EPTII HPKGHTIPRLDDKALEVMESFIQRISK L+ETEE
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 222

BLAST of HG10004481 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 311.6 bits (797), Expect = 7.9e-85
Identity = 143/223 (64.13%), Postives = 181/223 (81.17%), Query Frame = 0

Query: 126 MGTDGKFL-RKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPFPAEGKSDVE 185
           MG++G+ + RKPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 186 GIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQ 245
           GIFDPPY+EWFQF+ EFTEY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 246 AKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLGEEDFLMPHGQKLLE 305
           AKGIA  KVPKIKFVII+ GAK +S  +AENAYS+++   SLHFLGE DFL P+G +L+E
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIE 180

Query: 306 SYVEPTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDETEE 348
           SY  P +++HPKGHT+PRLD+K+LE + +FI  +   + E ++
Sbjct: 181 SYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHLVMEEDK 223

BLAST of HG10004481 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.3e-76
Identity = 132/216 (61.11%), Postives = 164/216 (75.93%), Query Frame = 0

Query: 134 RKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPFPAEGKSDVEGIFDPPYFE 193
           + PR LCLHGFRTS  IL+  +GKWP ++L  LDLDFLDAPFPA GKSDVE  FDPPY+E
Sbjct: 28  KNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYE 87

Query: 194 WFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKV 253
           W+Q +  F EYRNF+ECL++IEDYM+K+GPFDG LGFSQGA L+AA+PG Q +G ALTKV
Sbjct: 88  WYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKV 147

Query: 254 PKIKFVIIVSGAK-----FRSQSVAENAYSTAIGCPSLHFLGEEDFLMPHGQKLLESYVE 313
           PK+KF++I+SGAK     F     A NA+S+ + CPSLHF+GE DFL   G+ L+ES+VE
Sbjct: 148 PKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEVLVESFVE 207

Query: 314 PTIINHPKGHTIPRLDDKALEVMESFIQRISKTLDE 345
           P +I+H  GH IP+LD KA E M SF Q I + L +
Sbjct: 208 PVVIHHSGGHIIPKLDTKAEETMLSFFQSIRQMLSD 243

BLAST of HG10004481 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 248.8 bits (634), Expect = 6.3e-66
Identity = 116/166 (69.88%), Postives = 139/166 (83.73%), Query Frame = 0

Query: 126 MGTDGKFL-RKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLDFLDAPFPAEGKSDVE 185
           MG++G+ + RKPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 186 GIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQ 245
           GIFDPPY+EWFQF+ EFTEY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 246 AKGIALTKVPKIKFVIIVSGAKFRSQSVAENAYSTAIGCPSLHFLG 291
           AKGIA  KVPKIKFVII+ GAK +S  +AENAYS+++   SLHFLG
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

BLAST of HG10004481 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-12
Identity = 49/140 (35.00%), Postives = 68/140 (48.57%), Query Frame = 0

Query: 8   QRKPKILCLHGFRTSGAILKKQIGKWPNSVLNQFDFHFIDAP----FPSQGKSDVQGIYD 67
           +RK +ILCLHGFR + +  K + G     + N  +  FIDAP    F  Q  +   G+ +
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 68  PPYFEW-----FQHSKET---------------TGYENFESCLEFIENYMVEYGPFDGLL 124
              F W     F    ET               T  E F+  L +++    E GPFDG+L
Sbjct: 411 KK-FAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGIL 470