HG10004378 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10004378
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	cytochrome b5
Location	Chr08: 16433988 .. 16436807 (+)
RNA-Seq Expression	HG10004378
Synteny	HG10004378

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACAAACAATCCAAATACAACAAGAAAATGCTTGCAATGGTGGTTGTTGGCGGCAGCGGGCAACAACTATGGAAAGTGAAGGTAGTGTGAGGGTATTTTTGTCATTAAAAGTTGTTAGAGTTGCAATTAACGCACTTAAAATACCGTTTTTTTAATATAGCTTCAAGTGTTTATCTCACAATAGCTAATATCTAAATCTTTTTTTTTTAAAAAAAAAAACATTTTTAATGTTTGTCAAACCTTTTAATTTTTTTTCAAAGAGACTTATTTTCAATAATAAACACTTGATAAGGAAATCCAAATGGACTCACTCATGGTTTGATTATCAACTGATGTTTTGATGAGACCGGTGCTAGTTTGTACAAGAAATGTGGTATGAGTATTTGAACTTTTGATCTCATGTCAATTATCGCATAACTATATTTAATTACTTTGGTTGAAATGTGCTAGTTTGGATGTCTTAAGAGATGAGTTTATTTATGTACTCTCAAGAGGTGTACATGATATTATTTAACAACTATTTAAGATCGGTGTTACTACTTTTTTATACCGTGGTTACCATTTATTTCATCTAAAAAAAATTATATAATCAATTTTTTTCACATCAGTCAATTATATAAATGATGATGTAAACAATGTCAGGTAGGTCTTCCAACTTTTACATATTAACTGTAACATCAATTTTCTCTTATTTTAAATTTTCTAATGCTATTATTTAAAAAAGAATTCAAAGTTCAAACATAAAAATAAAACATTTTAAGTATCAAAGTTCAGATAACAAAATGATATTTTAAGCTAAAACAATCTTACCCTACAAATCCAGTAAGAAATGCTTAAATTGGGCCAGTCTCCAGATCCGGCCCAAGGATGGCTACCCTTATGGGCCAGCGAAGGAATGGCTGAGGCCCAAAGCTCACCAAAAAGCTTGATATTTCGAGAGCGAGTTCTTAAAAAAAAAATCATCCGATCACAGTCGAACCAACCCGCGGCGGCGATTAGTCGACCAATCGCCGAAGGGATCGATGCCGAGCATTTCGGCCCTCTATTCCATTCAAGAAGTCTCACAGCACAGTTCCAACGATGATTGTTGGATCATTATCGATGGCAAGGTACGTCCCTGATATTTGTTCATGGCGTTAAATAATTTTCTTTCGCAAATTGTTGTTCAGTTCGTCGCCCTTATTATTTTTTCTTCATATATTTGCTTTGTTATATGATTCTTATGTAATTTTCTTTCTGGTTTTTTTTTTGGTTTATGCTCGTGTTTGTTCTATTTGTGATTCTATGAGATGTGTTCTGAATTGTGGTTGGAATTTATTTAAGACGCCTTCGAGTTGTAGACTGAATTCAATTTTTATTGTTAATGATTGTCTTTTTGGGTTGGAGGAATGGGGATTTGATTCTATACTTTGACTGGAGTTAGCTGGATTTGTCAATGTGATGGTGAGAAAAGAAGCAAACAAGCTTATCATGTTCAGGTTCTTTGATTCATGTTGTTGATGGTTTGTAGGTTATCTGGATTTTAGATACATGTTGATTATGATAGCTGTAATTGATTAGGATGAAGACTTAATTCTAAATGAAATCCTGTTTAGAATAAAGTAGTTATCTGTTAATATAGGTTTGAATTATAAATTATTTTAAGAGTTGAGATCTGAGAAAGTCTATTGGCTTGAATTTCTCCTCTCCTCCTGAAATATCTTCTTTTATTCTCTAAGATTCGTTTTGATCCCACTCTTGGAGACAGAGTGGAGATAACCGGATTAAATTAGAAGGTTAAAGAAATGGAGGATGGATGGAAAACCCAACAACAAGGCTCTCTTGTGAATCAATAGATTTATTTTAATGCTCAATTCATTGTCATGTCAAAATTTTAGAATTAACAATACAAAAGTAAGGGGTTAGAGTACTGAATTCAAGTAAGACTAAAAGTTTCAATCTCCTCATAGAACTTATGTTAGTGTTTGAATCTGAGAATTTTCTTGAGTGGATATGTCAAATCCGATGATTTGTTGGTTTGAAATTCTTGAGAGGTAATTAACATTTCTCGTTGTGTCTTTGAAACATTGGCCCACCATTTCCAGAATCTTTTTAAACTTTATGTGCTCATTTTCTTTATGGCAGCCTAGTTTGTCAAAATTGTGAATATCTTTTTATGAAGGAATTGTGTTTGAAGATGATAAGGATGAATCTTGTCACTCCTATATTTCCTAACTCCCCATTTTTGCCTAAAAGAAGTGATACTTTCATTTTTACTTTCTAATAAAATTGAGATCCATGATCAATTTTTTTGTCAACGCTTTTCTCGATTGACACCATTCTATGACACGTCCACCTCCTTTAACCATTACATGTGTTTTCTTTTAACATATTTTGTTTAAGAGGATGAACTTTAACATTGCTTTATAATGACATGTTATCATTTTTAGGTATACGATTTGACTTCATATTTGGATGAGCATCCCGGTGGAGATGACATTATTGTGGCTGCAACTGGTATGCTTGTTCTTTGTAATGTATACTTCTCTCCCAAATCTGAACAGTGACAGATATATATACTTTTTTTCCATCAAAGGCAGAGATGCCACAGATGATTTTGAAGATGCCGGTCATAGCAAAGATGCCAGAGAGTTGATGGAGAAGTTCTATATCGGCGTGCTTGATACATCTTCCTCGGATAGCCTAGAACTTGAAACGAATCAAGTAGACGGTTATGCAGCTCGGGTTCAGGCCTTGACAAAGCAATACTGGAAGGCTCCTGTTGCAGTACTTGGTATTTCTGTGGTACTTGGCTGTCTATACTTGCGCAAAAAGTGA

mRNA sequence

ATGCACAAACAATCCAAATACAACAAGAAAATGCTTGCAATGGTGGTTGTTGGCGGCAGCGGGCAACAACTATGGAAAGTGAAGGTAGTTCGAACCAACCCGCGGCGGCGATTAGTCGACCAATCGCCGAAGGGATCGATGCCGAGCATTTCGGCCCTCTATTCCATTCAAGAAGTCTCACAGCACAGTTCCAACGATGATTGTTGGATCATTATCGATGGCAAGGTATACGATTTGACTTCATATTTGGATGAGCATCCCGGTGGAGATGACATTATTGTGGCTGCAACTGGCAGAGATGCCACAGATGATTTTGAAGATGCCGGTCATAGCAAAGATGCCAGAGAGTTGATGGAGAAGTTCTATATCGGCGTGCTTGATACATCTTCCTCGGATAGCCTAGAACTTGAAACGAATCAAGTAGACGGTTATGCAGCTCGGGTTCAGGCCTTGACAAAGCAATACTGGAAGGCTCCTGTTGCAGTACTTGGTATTTCTGTGGTACTTGGCTGTCTATACTTGCGCAAAAAGTGA

Coding sequence (CDS)

ATGCACAAACAATCCAAATACAACAAGAAAATGCTTGCAATGGTGGTTGTTGGCGGCAGCGGGCAACAACTATGGAAAGTGAAGGTAGTTCGAACCAACCCGCGGCGGCGATTAGTCGACCAATCGCCGAAGGGATCGATGCCGAGCATTTCGGCCCTCTATTCCATTCAAGAAGTCTCACAGCACAGTTCCAACGATGATTGTTGGATCATTATCGATGGCAAGGTATACGATTTGACTTCATATTTGGATGAGCATCCCGGTGGAGATGACATTATTGTGGCTGCAACTGGCAGAGATGCCACAGATGATTTTGAAGATGCCGGTCATAGCAAAGATGCCAGAGAGTTGATGGAGAAGTTCTATATCGGCGTGCTTGATACATCTTCCTCGGATAGCCTAGAACTTGAAACGAATCAAGTAGACGGTTATGCAGCTCGGGTTCAGGCCTTGACAAAGCAATACTGGAAGGCTCCTGTTGCAGTACTTGGTATTTCTGTGGTACTTGGCTGTCTATACTTGCGCAAAAAGTGA

Protein sequence

MHKQSKYNKKMLAMVVVGGSGQQLWKVKVVRTNPRRRLVDQSPKGSMPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFEDAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGISVVLGCLYLRKK

Homology

[Close]

BLAST of HG10004378 vs. NCBI nr
Match: XP_038884358.1 (cytochrome b5 [Benincasa hispida])

HSP 1 Score: 256.9 bits (655), Expect = 1.3e-64
Identity = 126/131 (96.18%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDD+IVAATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGIS 166
           DAGHSKDARELMEKFYIG+LDTSSSDSL+LET+QVDGYAARVQA TKQYWKAPVAVLGIS
Sbjct: 61  DAGHSKDARELMEKFYIGMLDTSSSDSLKLETSQVDGYAARVQASTKQYWKAPVAVLGIS 120

Query: 167 VVLGCLYLRKK 178
           VVLGCLYLRKK
Sbjct: 121 VVLGCLYLRKK 131

[Close]

BLAST of HG10004378 vs. NCBI nr
Match: XP_004144166.1 (cytochrome b5 [Cucumis sativus] >KGN47656.1 hypothetical protein Csa_018326 [Cucumis sativus])

HSP 1 Score: 248.8 bits (634), Expect = 3.4e-62
Identity = 122/131 (93.13%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDD+IVAATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGIS 166
           DAGHSKDARELMEKFYIG+LDTSS DSL+LETNQVDGYA RVQ LTKQYWKAPVAV+GIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120

Query: 167 VVLGCLYLRKK 178
           VVLG  YLRKK
Sbjct: 121 VVLGWAYLRKK 131

[Close]

BLAST of HG10004378 vs. NCBI nr
Match: XP_008445442.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 245.4 bits (625), Expect = 3.8e-61
Identity = 120/131 (91.60%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSIQEVSQHSS+DDCWIIIDGKVYDLTSYLDEHPGGDDIIV ATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSSDDCWIIIDGKVYDLTSYLDEHPGGDDIIVTATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGIS 166
           DAGHSKDARELMEKFYIG+LDTSSSDSL+LETNQVD YA RVQ LTKQYWKAPVAV+GIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSSDSLKLETNQVDSYATRVQTLTKQYWKAPVAVIGIS 120

Query: 167 VVLGCLYLRKK 178
           +VLG +YLRKK
Sbjct: 121 MVLGWVYLRKK 131

[Close]

BLAST of HG10004378 vs. NCBI nr
Match: KAG6598642.1 (Cytochrome b5 isoform A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 231.1 bits (588), Expect = 7.4e-57
Identity = 115/131 (87.79%), Postives = 119/131 (90.84%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSI+EVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDD+IVAATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGIS 166
           DAGHSKDARELMEKFYIG LD SSS   ELET + DGYAARVQ LTKQYW  P+AVLGIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120

Query: 167 VVLGCLYLRKK 178
           VVLG LYL KK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of HG10004378 vs. NCBI nr
Match: XP_022961879.1 (cytochrome b5 [Cucurbita moschata])

HSP 1 Score: 228.8 bits (582), Expect = 3.7e-56
Identity = 114/131 (87.02%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSI+EVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDD+IVAATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGIS 166
           DAGHSKDARELMEKFYIG LD SSS   ELE  + DGYAARVQ LTKQYW  P+AVLGIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELEAKEPDGYAARVQTLTKQYWGIPIAVLGIS 120

Query: 167 VVLGCLYLRKK 178
           VVLG LYL KK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of HG10004378 vs. ExPASy Swiss-Prot
Match: Q9FDW8 (Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.6e-38
Identity = 76/134 (56.72%), Postives = 102/134 (76.12%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MP+++ LYS++E + H+  DDCW++IDGKVYD++SY+DEHPGGDD+++A  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSL-ELETNQVDGYAARVQA---LTKQYWKAPVAV 166
           DAGHSKDARELMEK++IG LD SS   + EL+  + D     VQ    LTKQYW  PV++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 167 LGISVVLGCLYLRK 177
           + ISV +  L+ RK
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of HG10004378 vs. ExPASy Swiss-Prot
Match: Q9ZWT2 (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.5e-25
Identity = 45/78 (57.69%), Postives = 68/78 (87.18%), Query Frame = 0


Query: 53  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFEDAGHSK 112
           ++++ EVSQHSS  DCWI+IDGKVYD+T +LD+HPGGD++I+ +TG+DATDDFED GHS 
Sbjct: 7   VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 113 DARELMEKFYIGVLDTSS 131
            A+ +++++Y+G +DT++
Sbjct: 67  TAKAMLDEYYVGDIDTAT 84

[Close]

BLAST of HG10004378 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-24
Identity = 55/134 (41.04%), Postives = 85/134 (63.43%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           M S   +   +EVSQH+   DCW+II GKVYD+T ++D+HPGGD++++++TG+DAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQ-----------Y 166
           D GHS  AR++MEK+YIG +D+S+  +          Y A VQ    Q            
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRT-------YVAPVQPAYNQDKTPEFMIKILQ 120

Query: 167 WKAPVAVLGISVVL 170
           +  P+ +LG+++V+
Sbjct: 121 FLVPILILGLALVV 127

[Close]

BLAST of HG10004378 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-24
Identity = 43/78 (55.13%), Postives = 70/78 (89.74%), Query Frame = 0


Query: 53  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFEDAGHSK 112
           +Y+++EV++H+S DDCW+II GKVY+++ +L++HPGGDD+++++TG+DATDDFED GHS 
Sbjct: 8   VYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHST 67

Query: 113 DARELMEKFYIGVLDTSS 131
            AR +M+++Y+G +DTS+
Sbjct: 68  TARAMMDEYYVGDIDTST 85

[Close]

BLAST of HG10004378 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.0e-24
Identity = 42/78 (53.85%), Postives = 69/78 (88.46%), Query Frame = 0


Query: 53  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFEDAGHSK 112
           ++++ EVS+H+   DCWI+I+GKVY++T +L++HPGGDD+++++TG+DATDDFED GHS+
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSE 66

Query: 113 DARELMEKFYIGVLDTSS 131
            ARE+ME++Y+G +D ++
Sbjct: 67  SAREMMEQYYVGEIDPTT 84

[Close]

BLAST of HG10004378 vs. ExPASy TrEMBL
Match: A0A0A0KIR1 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G366540 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.7e-62
Identity = 122/131 (93.13%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDD+IVAATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGIS 166
           DAGHSKDARELMEKFYIG+LDTSS DSL+LETNQVDGYA RVQ LTKQYWKAPVAV+GIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120

Query: 167 VVLGCLYLRKK 178
           VVLG  YLRKK
Sbjct: 121 VVLGWAYLRKK 131

[Close]

BLAST of HG10004378 vs. ExPASy TrEMBL
Match: A0A1S3BCR4 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.8e-61
Identity = 120/131 (91.60%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSIQEVSQHSS+DDCWIIIDGKVYDLTSYLDEHPGGDDIIV ATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSSDDCWIIIDGKVYDLTSYLDEHPGGDDIIVTATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGIS 166
           DAGHSKDARELMEKFYIG+LDTSSSDSL+LETNQVD YA RVQ LTKQYWKAPVAV+GIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSSDSLKLETNQVDSYATRVQTLTKQYWKAPVAVIGIS 120

Query: 167 VVLGCLYLRKK 178
           +VLG +YLRKK
Sbjct: 121 MVLGWVYLRKK 131

[Close]

BLAST of HG10004378 vs. ExPASy TrEMBL
Match: A0A6J1HFA7 (cytochrome b5 OS=Cucurbita moschata OX=3662 GN=LOC111462520 PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.8e-56
Identity = 114/131 (87.02%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSI+EVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDD+IVAATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGIS 166
           DAGHSKDARELMEKFYIG LD SSS   ELE  + DGYAARVQ LTKQYW  P+AVLGIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELEAKEPDGYAARVQTLTKQYWGIPIAVLGIS 120

Query: 167 VVLGCLYLRKK 178
           VVLG LYL KK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of HG10004378 vs. ExPASy TrEMBL
Match: A0A5A7V8T8 (Cytochrome b5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00770 PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.4e-55
Identity = 109/118 (92.37%), Postives = 113/118 (95.76%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSIQEVSQHSS+DDCWIIIDGKVYDLTSYLDEHPGGDDIIV ATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSSDDCWIIIDGKVYDLTSYLDEHPGGDDIIVTATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLG 165
           DAGHSKDARELMEKFYIG+LDTSSSDSL+LETNQVD YA  VQ LTKQYWKAPVAV+G
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSSDSLKLETNQVDSYATLVQTLTKQYWKAPVAVIG 118

[Close]

BLAST of HG10004378 vs. ExPASy TrEMBL
Match: A0A6J1KB15 (cytochrome b5 OS=Cucurbita maxima OX=3661 GN=LOC111492240 PE=3 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.8e-54
Identity = 108/131 (82.44%), Postives = 117/131 (89.31%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MPSISALYSI+E+S+HSSNDDCWIIIDGKVYDLTSYLDEHPGGDD+I+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEISRHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIIAATGRDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQYWKAPVAVLGIS 166
           DAGHSKD RELM+KFYIG LD SSS    LET + DGYAA+VQ LTKQYW  P+AVLGIS
Sbjct: 61  DAGHSKDTRELMKKFYIGELDPSSSAIPALETKEPDGYAAQVQTLTKQYWGIPIAVLGIS 120

Query: 167 VVLGCLYLRKK 178
           VVLG LYL KK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of HG10004378 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 160.6 bits (405), Expect = 1.1e-39
Identity = 76/134 (56.72%), Postives = 102/134 (76.12%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           MP+++ LYS++E + H+  DDCW++IDGKVYD++SY+DEHPGGDD+++A  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSL-ELETNQVDGYAARVQA---LTKQYWKAPVAV 166
           DAGHSKDARELMEK++IG LD SS   + EL+  + D     VQ    LTKQYW  PV++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 167 LGISVVLGCLYLRK 177
           + ISV +  L+ RK
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of HG10004378 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 116.3 bits (290), Expect = 2.5e-26
Identity = 45/78 (57.69%), Postives = 68/78 (87.18%), Query Frame = 0


Query: 53  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFEDAGHSK 112
           ++++ EVSQHSS  DCWI+IDGKVYD+T +LD+HPGGD++I+ +TG+DATDDFED GHS 
Sbjct: 7   VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 113 DARELMEKFYIGVLDTSS 131
            A+ +++++Y+G +DT++
Sbjct: 67  TAKAMLDEYYVGDIDTAT 84

[Close]

BLAST of HG10004378 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 113.2 bits (282), Expect = 2.1e-25
Identity = 42/78 (53.85%), Postives = 69/78 (88.46%), Query Frame = 0


Query: 53  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFEDAGHSK 112
           ++++ EVS+H+   DCWI+I+GKVY++T +L++HPGGDD+++++TG+DATDDFED GHS+
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSE 66

Query: 113 DARELMEKFYIGVLDTSS 131
            ARE+ME++Y+G +D ++
Sbjct: 67  SAREMMEQYYVGEIDPTT 84

[Close]

BLAST of HG10004378 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 112.1 bits (279), Expect = 4.7e-25
Identity = 53/129 (41.09%), Postives = 87/129 (67.44%), Query Frame = 0


Query: 47  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFE 106
           M S   + S +EVS+H+   DCW+II GKVYD+T ++D+HPGGD++++++TG+DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 107 DAGHSKDARELMEKFYIGVLDTSSSDSLELETNQVDGYAARVQALTKQY------WKAPV 166
           D GHS  AR++M+K++IG +D+SS  +    T       A  Q  T ++      +  P+
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPA--TRTYVAPQQPAYNQDKTPEFIIKILQFLVPI 120

Query: 167 AVLGISVVL 170
            +LG+++V+
Sbjct: 121 LILGLALVV 127

[Close]

BLAST of HG10004378 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 105.1 bits (261), Expect = 5.7e-23
Identity = 43/78 (55.13%), Postives = 64/78 (82.05%), Query Frame = 0


Query: 53  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDIIVAATGRDATDDFEDAGHSK 112
           L S  +V++H   +DCWI+I GKVYD+++++DEHPGGD++++A TG+DA+ DFED  HSK
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 113 DARELMEKFYIGVLDTSS 131
           DA+ELM+K+ IG +D S+
Sbjct: 64  DAKELMKKYCIGDVDQST 81

The following BLAST results are available for this feature:

BLAST of HG10004378 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884358.1	1.3e-64	96.18	cytochrome b5 [Benincasa hispida]	[more]
XP_004144166.1	3.4e-62	93.13	cytochrome b5 [Cucumis sativus] >KGN47656.1 hypothetical protein Csa_018326 [Cuc...	[more]
XP_008445442.1	3.8e-61	91.60	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
KAG6598642.1	7.4e-57	87.79	Cytochrome b5 isoform A, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022961879.1	3.7e-56	87.02	cytochrome b5 [Cucurbita moschata]	[more]

BLAST of HG10004378 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FDW8	1.6e-38	56.72	Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1	[more]
Q9ZWT2	3.5e-25	57.69	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]
P40934	1.3e-24	41.04	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49100	1.3e-24	55.13	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
O48845	3.0e-24	53.85	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]

BLAST of HG10004378 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIR1	1.7e-62	93.13	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A1S3BCR4	1.8e-61	91.60	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1	[more]
A0A6J1HFA7	1.8e-56	87.02	cytochrome b5 OS=Cucurbita moschata OX=3662 GN=LOC111462520 PE=3 SV=1	[more]
A0A5A7V8T8	3.4e-55	92.37	Cytochrome b5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00770 P...	[more]
A0A6J1KB15	2.8e-54	82.44	cytochrome b5 OS=Cucurbita maxima OX=3661 GN=LOC111492240 PE=3 SV=1	[more]

BLAST of HG10004378 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26340.1	1.1e-39	56.72	cytochrome B5 isoform A	[more]
AT5G48810.1	2.5e-26	57.69	cytochrome B5 isoform D	[more]
AT2G32720.1	2.1e-25	53.85	cytochrome B5 isoform B	[more]
AT5G53560.1	4.7e-25	41.09	cytochrome B5 isoform E	[more]
AT2G46650.1	5.7e-23	55.13	cytochrome B5 isoform C	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 76..86 score: 55.65 coord: 114..126 score: 43.47 coord: 101..108 score: 44.7 coord: 86..100 score: 49.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 54..127 e-value: 6.2E-32 score: 122.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 57..126 e-value: 3.2E-25 score: 88.0
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 51..127 score: 28.729862
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 51..139 e-value: 1.2E-33 score: 117.0
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 49..130
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 37..176
None	No IPR available	PANTHER	PTHR19359:SF25	CYTOCHROME B5 ISOFORM A	coord: 37..176
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 82..89

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10004378.1	HG10004378.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009707	chloroplast outer membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0010319	stromule
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding