HG10004309 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10004309 vs. NCBI nr
Match: XP_004143163.1 (asparagine synthetase [glutamine-hydrolyzing] [Cucumis sativus] >KGN47067.1 hypothetical protein Csa_020758 [Cucumis sativus])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 532/589 (90.32%), Postives = 548/589 (93.04%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQH DCYLAHQRLAIIDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHKDCYLAHQRLAIIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNED+S+VVTVNGEIYNHEELRKKLRNH+IRTGSDCDVISHLYE+YGENFVDMLD
Sbjct: 61  GDQPLYNEDQSVVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+ ARDAIGITSLYIGWGLDGSVWISSELK LNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SKEA FKRWYNPTWFSEDIPSTPYDP+VLRRA ENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITARHL GTRAAKHWGTQLHSFCVGLEGSPDLKAA+EVADFLGTVHHEFHFTVQD
Sbjct: 241 SLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMVISGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRESCRK                         PFLDKEF NVAMAIDPEWKMIK
Sbjct: 361 PNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            DQG+IEKWVLR+AFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKMM
Sbjct: 421 RDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN
Sbjct: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVHA+AYGDE NGVN V PPK ID++PRMEVS PGVKILS
Sbjct: 541 LDPSGRAALGVHAAAYGDETNGVNNVAPPKIIDSIPRMEVSTPGVKILS 587

BLAST of HG10004309 vs. NCBI nr
Match: XP_008465262.1 (PREDICTED: asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucumis melo])

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 533/589 (90.49%), Postives = 549/589 (93.21%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQH DCYLAHQRLAIIDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHKDCYLAHQRLAIIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNH+IRTGSDCDVISHLYE+YGENFVDMLD
Sbjct: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+ ARDAIGITSLYIGWGLDGSVWISSELK LNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SKEA FKRWYNPTWFSEDIPSTPYDP+VLRRALENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITARHL GTRAAKHWGTQLHSFCVGLEGSPDLKAA+EVADFLGTVHHEFHFTVQD
Sbjct: 241 SLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMVISGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRESCRK                         PFLDKEF NVAMAIDPEWKMIK
Sbjct: 361 PNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            DQG+IEKWVLR+AFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKMM
Sbjct: 421 RDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKA+EWDAAWSKN
Sbjct: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVHA+AYGDEANGVN + PPK ID++PRMEV  PGVKILS
Sbjct: 541 LDPSGRAALGVHAAAYGDEANGVN-IAPPKIIDSIPRMEVGTPGVKILS 586

BLAST of HG10004309 vs. NCBI nr
Match: XP_004144212.3 (asparagine synthetase [glutamine-hydrolyzing] isoform X1 [Cucumis sativus])

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 525/589 (89.13%), Postives = 551/589 (93.55%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQHADCYL+HQRLAI+DPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHADCYLSHQRLAIVDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNE+KSIVVTVNGEIYNHEELRKKLRNH+IRT SDCDVISHLYE+YGENFVDMLD
Sbjct: 61  GDQPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+AARDAIG+TSLYIGWG DGSVWISSELK LNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SKEAGFKRWYNP WFSEDIPSTPYDP+VLRRALENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITAR+L GTRAAKHWGTQLHSFCVGLEGSPDLKAA+EVADFLGTVHHEFHFTVQD
Sbjct: 241 SLVASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMV+SGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRESCRK                         PFLDK+F +VAMAIDPEWKMIK
Sbjct: 361 PNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
           H+QG+IEKWVLR+AFDDEQQPYLPK+VLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKM+
Sbjct: 421 HNQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMI 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSA LTVPGGPSIACSTAKAVEWDAAWSKN
Sbjct: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVHAS YGDEANG+N++VPPK +DT+PRMEVS  GVKILS
Sbjct: 541 LDPSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 587

BLAST of HG10004309 vs. NCBI nr
Match: KAE8647225.1 (hypothetical protein Csa_018945 [Cucumis sativus])

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 525/589 (89.13%), Postives = 551/589 (93.55%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQHADCYL+HQRLAI+DPAS
Sbjct: 51  MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHADCYLSHQRLAIVDPAS 110

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNE+KSIVVTVNGEIYNHEELRKKLRNH+IRT SDCDVISHLYE+YGENFVDMLD
Sbjct: 111 GDQPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLD 170

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+AARDAIG+TSLYIGWG DGSVWISSELK LNDDCEHFETFPPGH
Sbjct: 171 GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH 230

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SKEAGFKRWYNP WFSEDIPSTPYDP+VLRRALENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 231 LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 290

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITAR+L GTRAAKHWGTQLHSFCVGLEGSPDLKAA+EVADFLGTVHHEFHFTVQD
Sbjct: 291 SLVASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQD 350

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMV+SGEGADEIFGGYLYFHKA
Sbjct: 351 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKA 410

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRESCRK                         PFLDK+F +VAMAIDPEWKMIK
Sbjct: 411 PNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIK 470

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
           H+QG+IEKWVLR+AFDDEQQPYLPK+VLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKM+
Sbjct: 471 HNQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMI 530

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSA LTVPGGPSIACSTAKAVEWDAAWSKN
Sbjct: 531 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSACLTVPGGPSIACSTAKAVEWDAAWSKN 590

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVHAS YGDEANG+N++VPPK +DT+PRMEVS  GVKILS
Sbjct: 591 LDPSGRAALGVHASTYGDEANGLNDIVPPKIVDTIPRMEVSTAGVKILS 637

BLAST of HG10004309 vs. NCBI nr
Match: XP_038886005.1 (asparagine synthetase [glutamine-hydrolyzing] 1-like [Benincasa hispida])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 527/589 (89.47%), Postives = 547/589 (92.87%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQHAD YL+HQRLAIIDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHADFYLSHQRLAIIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNED+SIVVTVNGEIYNHEELRKKLRNH+IRT SDCDVISHLYE+YGENFVDMLD
Sbjct: 61  GDQPLYNEDESIVVTVNGEIYNHEELRKKLRNHKIRTDSDCDVISHLYEEYGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+AARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SKEAGFKRWYNPTWFSEDIPSTPYDP+VLR ALENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEAGFKRWYNPTWFSEDIPSTPYDPLVLRCALENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SL+ASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAA+EVADFLGTVHHEFHFTVQD
Sbjct: 241 SLIASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYH+ETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHVETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRESCRK                         PFLDKEF NVAMAIDPEWKMIK
Sbjct: 361 PNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
           HDQG+IEKWVLR+AF+DEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKMM
Sbjct: 421 HDQGRIEKWVLRRAFNDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           +NAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAR TVPGGPSIACSTAKAVEWDA WSKN
Sbjct: 481 VNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARQTVPGGPSIACSTAKAVEWDATWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVHASAYGDE N +N+VVPPK ID + RM VS P V+ILS
Sbjct: 541 LDPSGRAALGVHASAYGDEVNHINDVVPPKIIDPISRMNVSTPEVEILS 587

BLAST of HG10004309 vs. ExPASy Swiss-Prot
Match: P49078 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 GN=ASN1 PE=2 SV=2)

HSP 1 Score: 959.1 bits (2478), Expect = 2.2e-278
Identity = 456/578 (78.89%), Postives = 508/578 (87.89%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  L+HRGPDWSGLYQ+ D YLAHQRLA+IDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LRHRGPDWSGLYQNGDNYLAHQRLAVIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPL+NEDK+IVVTVNGEIYNHEELRK+L+NH+ RTGSDC+VI+HLYE+YG +FVDMLD
Sbjct: 61  GDQPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           G+FSFVLLDTRDNSFM ARDAIG+TSLYIGWGLDGSVWISSE+K LNDDCEHFETFPPGH
Sbjct: 121 GIFSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
            Y+SK  GFK+WYNP WF+E +PSTPY+P+ +RRA ENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 FYSSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITARHLAGT+AAK WG QLHSFCVGLEGSPDLKA KEVA++LGTVHHEFHF+VQD
Sbjct: 241 SLVASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNK+EFH+E+CRK                         PFLDK+F N AM++DPE KMIK
Sbjct: 361 PNKKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            ++G+IEKWVLR+AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA Q+V DKMM
Sbjct: 421 PEEGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVNDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
            NA HIFP NTP +KEAYYYR IFERFFPQNSARLTVPGG ++ACSTAKAVEWDA+WS N
Sbjct: 481 SNAGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDASWSNN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRM 554
           +DPSGRAA+GVH SAY D  N    + P K ID +P M
Sbjct: 541 MDPSGRAAIGVHLSAY-DGKNVALTIPPLKAIDNMPMM 575

BLAST of HG10004309 vs. ExPASy Swiss-Prot
Match: O24661 (Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor OX=64093 GN=AS PE=2 SV=3)

HSP 1 Score: 958.0 bits (2475), Expect = 4.8e-278
Identity = 460/575 (80.00%), Postives = 505/575 (87.83%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSG++ H DCYLAHQRLAI+DPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGIHHHGDCYLAHQRLAIVDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPL+NEDK I VTVNGEIYNHEELR  L NH+ RTGSDCDVI+HLYE+YGENFV+MLD
Sbjct: 61  GDQPLFNEDKRIAVTVNGEIYNHEELRALLPNHKFRTGSDCDVIAHLYEEYGENFVEMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLD+RDN+F+AARDA GITSLYIGWGLDGSVWISSELK L+D+CE+FE FPPGH
Sbjct: 121 GMFSFVLLDSRDNTFIAARDAFGITSLYIGWGLDGSVWISSELKGLHDECENFEVFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           +Y+SK  GF+RWYNP WFSE IPSTPYDP+VLR A E AVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 VYSSKTEGFRRWYNPPWFSEAIPSTPYDPLVLRGAFEQAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVA++TARHLAGT+AAK WG+QLHSFCVGLEGSPDLKA KEVAD+LGTVHHEF FTVQD
Sbjct: 241 SLVAAVTARHLAGTKAAKRWGSQLHSFCVGLEGSPDLKAGKEVADYLGTVHHEFLFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYHIETYDVTTIRASTPMFLM+RKIKSLGVKMVISGEG+DEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRE+CRK                         PFLDKEF N+AM+IDPE KMIK
Sbjct: 361 PNKEEFHRETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFVNLAMSIDPEAKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            DQG+IEKW+LRKAFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHA+QHVTDKMM
Sbjct: 421 PDQGRIEKWILRKAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAEQHVTDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNA HIFP NTPT+KE YYYR IFERFFPQNSA+LTVPGGPS+ACSTA AV WDA+WSKN
Sbjct: 481 LNAGHIFPHNTPTTKEGYYYRMIFERFFPQNSAKLTVPGGPSVACSTATAVAWDASWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTV 551
           +DPSGRAA GVH  AY +     N  +  K++D+V
Sbjct: 541 LDPSGRAATGVHDLAYENHVPIGN--LKSKKMDSV 571

BLAST of HG10004309 vs. ExPASy Swiss-Prot
Match: P49092 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=AS1 PE=2 SV=2)

HSP 1 Score: 951.8 bits (2459), Expect = 3.4e-276
Identity = 457/589 (77.59%), Postives = 512/589 (86.93%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSD +QAKRVRVLELSRR  LKHRGPDWSGL+QH DCYLAHQRLAI+DPAS
Sbjct: 1   MCGILAVLGCSDFTQAKRVRVLELSRR--LKHRGPDWSGLHQHGDCYLAHQRLAIVDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPL+NEDKSI+VTVNGEIYNHEELRK+L NH+ RTGSDCDVI+HLYE++GENF+DMLD
Sbjct: 61  GDQPLFNEDKSIIVTVNGEIYNHEELRKQLPNHQFRTGSDCDVIAHLYEEHGENFMDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           G+FSFVLLDTRDN+F+ ARDAIG+TSLYIGWGLDGSVWISSE+K LNDDCEHFE FPPGH
Sbjct: 121 GIFSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFEVFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+S+E  F+RWYNPTWFSE IPS PYDP+ +R A E AVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSRERAFRRWYNPTWFSESIPSAPYDPLAVRHAFEKAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASIT+R+LA T+AA+ WG++LHSFCVGLEGSPDLKAAKEVAD+LGTVHHEF FTVQD
Sbjct: 241 SLVASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIE+VIYH+ETYDVTTIRASTPMFLM+RKIKSLGVK VISGEG+DEIFGGYLYFHKA
Sbjct: 301 GIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFH E+CRK                         PFLDKEF NVAM IDPE+KMIK
Sbjct: 361 PNKEEFHTETCRKIKALHQYDCLRANKSTFAWGLEARVPFLDKEFINVAMNIDPEYKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            D+G+IEK++LR+AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA +HVTDKM+
Sbjct: 421 RDEGRIEKYILRRAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAKHVTDKMI 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNA +IF  NTP +KEAYYYR IFERFFPQNSARLTVPGGP++ACSTAKAVEWDAAWS N
Sbjct: 481 LNAGNIFRHNTPLTKEAYYYRMIFERFFPQNSARLTVPGGPTVACSTAKAVEWDAAWSNN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVH SAY D+ N +    P +    +P ME  + GV I S
Sbjct: 541 LDPSGRAALGVHLSAYDDKQNNLINNKPVEFEKLIP-MEAPSLGVAIHS 586

BLAST of HG10004309 vs. ExPASy Swiss-Prot
Match: P31752 (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 PE=2 SV=2)

HSP 1 Score: 951.4 bits (2458), Expect = 4.5e-276
Identity = 451/556 (81.12%), Postives = 498/556 (89.57%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGL QH DC+L+HQRLAIIDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLCQHGDCFLSHQRLAIIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNEDKSIVVTVNGEIYNHEELR++L +H+ RTGSDC+VI+HLYE++GE+FVDMLD
Sbjct: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRRRLPDHKYRTGSDCEVIAHLYEEHGEDFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTR+N F+AARDA+GIT LYIGWGLDGSVW+SSE+K LNDDCEHFE FPPG+
Sbjct: 121 GMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVWLSSEMKGLNDDCEHFEVFPPGN 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+S+   F+RWYNP W++E IPS PYDP+VLR+A E+AVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVA++TARHLAG++AA+ WGTQLHSFCVGLEGSPDLKAAKEVA++LGTVHHEFHFTVQD
Sbjct: 241 SLVAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLKAAKEVAEYLGTVHHEFHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVI+HIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEG+DEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFH E+CRK                         PFLDKEF +VAM+IDPE KMIK
Sbjct: 361 PNKEEFHHETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFMDVAMSIDPESKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            D G+IEKWVLRKAFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLKAHA +HVTD+MM
Sbjct: 421 PDLGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAKHVTDRMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 532
           LNA  I+P NTPT+KEAYYYR IFERFFPQNSAR TVPGGPSIACSTAKA+EWDA WS N
Sbjct: 481 LNAARIYPHNTPTTKEAYYYRMIFERFFPQNSARFTVPGGPSIACSTAKAIEWDARWSNN 540

BLAST of HG10004309 vs. ExPASy Swiss-Prot
Match: P49093 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=AS2 PE=2 SV=2)

HSP 1 Score: 945.7 bits (2443), Expect = 2.5e-274
Identity = 453/590 (76.78%), Postives = 513/590 (86.95%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGL+QH D +LAHQRLAI+DPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLHQHGDNFLAHQRLAIVDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPL+NED+SI+VTVNGEI+NHEELRK+L NH+ RTG DCDVI+HLYE++GENFVDMLD
Sbjct: 61  GDQPLFNEDQSIIVTVNGEIFNHEELRKQLPNHKFRTGCDCDVIAHLYEEHGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           G+FSFVLLDTRDNSF+ ARDAIG+TSLYIG+GLDGSVWI+SELK LNDDCEHFE FPPGH
Sbjct: 121 GIFSFVLLDTRDNSFLVARDAIGVTSLYIGYGLDGSVWIASELKGLNDDCEHFELFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SKE  F+RWYNP WFSE IPS PYDP+ LR+A E A+IKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEKEFRRWYNPPWFSEAIPSAPYDPLALRQAFEKAIIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVAS+TAR+LA T+AAK WG++LHSFCVGLEG+PDLKAA+EVAD++GTVHHEF +T+QD
Sbjct: 241 SLVASVTARYLADTKAAKQWGSKLHSFCVGLEGAPDLKAAREVADYIGTVHHEFQYTIQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYH+ETYDVTTIRA TPMFLM+RKIKSLGVKMV+SGEG+DEIF GYLYFHKA
Sbjct: 301 GIDAIEDVIYHVETYDVTTIRAGTPMFLMSRKIKSLGVKMVLSGEGSDEIFAGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEE H+E+C K                         PFLDK+F +VAM IDPE KMIK
Sbjct: 361 PNKEELHQETCSKIKALHKYDCLRANKSTYAWGLEARVPFLDKKFIDVAMGIDPENKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            D+G+IEKWVLRKAFDDE+ PYLPKH+LYRQKEQFSDGVGY WIDGLK HA +HVTDKMM
Sbjct: 421 RDEGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYGWIDGLKDHAAKHVTDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNA +I+P NTP +KEAYYYR IFERFFPQNSARL+VPGG SIACST KA+EWDAAWS N
Sbjct: 481 LNASNIYPFNTPNTKEAYYYRMIFERFFPQNSARLSVPGGASIACSTEKAIEWDAAWSNN 540

Query: 541 IDPSGRAALGVHASAYGDEAN-GVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVH SAY D+ N  V++ V P++I  +P+MEVS  GV ILS
Sbjct: 541 LDPSGRAALGVHDSAYDDQLNKSVSKGVEPEKI--IPKMEVSPLGVAILS 586

BLAST of HG10004309 vs. ExPASy TrEMBL
Match: A0A0A0KF94 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_6G183190 PE=4 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 532/589 (90.32%), Postives = 548/589 (93.04%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQH DCYLAHQRLAIIDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHKDCYLAHQRLAIIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNED+S+VVTVNGEIYNHEELRKKLRNH+IRTGSDCDVISHLYE+YGENFVDMLD
Sbjct: 61  GDQPLYNEDQSVVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+ ARDAIGITSLYIGWGLDGSVWISSELK LNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SKEA FKRWYNPTWFSEDIPSTPYDP+VLRRA ENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITARHL GTRAAKHWGTQLHSFCVGLEGSPDLKAA+EVADFLGTVHHEFHFTVQD
Sbjct: 241 SLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMVISGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRESCRK                         PFLDKEF NVAMAIDPEWKMIK
Sbjct: 361 PNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            DQG+IEKWVLR+AFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKMM
Sbjct: 421 RDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN
Sbjct: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVHA+AYGDE NGVN V PPK ID++PRMEVS PGVKILS
Sbjct: 541 LDPSGRAALGVHAAAYGDETNGVNNVAPPKIIDSIPRMEVSTPGVKILS 587

BLAST of HG10004309 vs. ExPASy TrEMBL
Match: A0A1S3CNG7 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC103502918 PE=4 SV=1)

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 533/589 (90.49%), Postives = 549/589 (93.21%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQH DCYLAHQRLAIIDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHKDCYLAHQRLAIIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNH+IRTGSDCDVISHLYE+YGENFVDMLD
Sbjct: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+ ARDAIGITSLYIGWGLDGSVWISSELK LNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SKEA FKRWYNPTWFSEDIPSTPYDP+VLRRALENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITARHL GTRAAKHWGTQLHSFCVGLEGSPDLKAA+EVADFLGTVHHEFHFTVQD
Sbjct: 241 SLVASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYHIETYDVTTIRASTPM+LMARKIKSLGVKMVISGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRESCRK                         PFLDKEF NVAMAIDPEWKMIK
Sbjct: 361 PNKEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            DQG+IEKWVLR+AFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKMM
Sbjct: 421 RDQGRIEKWVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKA+EWDAAWSKN
Sbjct: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAIEWDAAWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVHA+AYGDEANGVN + PPK ID++PRMEV  PGVKILS
Sbjct: 541 LDPSGRAALGVHAAAYGDEANGVN-IAPPKIIDSIPRMEVGTPGVKILS 586

BLAST of HG10004309 vs. ExPASy TrEMBL
Match: A0A5D3BR39 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00140 PE=4 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 525/614 (85.50%), Postives = 547/614 (89.09%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQH DCYL+HQRLAI+DPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHKDCYLSHQRLAIVDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNE+KSIVVTVNGEIYNHEELRKKL+NH+IRTGSDCDVISHLYE+YGENFVDMLD
Sbjct: 61  GDQPLYNENKSIVVTVNGEIYNHEELRKKLKNHKIRTGSDCDVISHLYEEYGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+AARDAIG+TSLYIGWG DGSVWISSELK LNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SKEAGFKRWYNP WFSEDIPSTPYDP+VLRRALENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLE--------------------------GS 300
           SLVASITARHL GTRAAKHWGTQLHSFCVGLE                          GS
Sbjct: 241 SLVASITARHLTGTRAAKHWGTQLHSFCVGLEHVFQELLFNPCNVISLHPSSFIHQFKGS 300

Query: 301 PDLKAAKEVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK 360
           PDLKAA+EVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK
Sbjct: 301 PDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK 360

Query: 361 SLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCRK--------------------- 420
           SLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESC K                     
Sbjct: 361 SLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCHKIKALHMYDCLRANKATSAWGL 420

Query: 421 ----PFLDKEFFNVAMAIDPEWKMIKHDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQ 480
               PFLDK+F  VAMAIDPEWKMIKHDQG+IEKWVLR+AFDDEQQPYLPK+VLYRQKEQ
Sbjct: 421 EIRVPFLDKKFIEVAMAIDPEWKMIKHDQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQ 480

Query: 481 FSDGVGYSWIDGLKAHADQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAR 540
           FSDGVGYSWIDGLKAHADQHVTDKM+LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSA 
Sbjct: 481 FSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSAC 540

Query: 541 LTVPGGPSIACSTAKAVEWDAAWSKNIDPSGRAALGVHASAYGDEANGVNEVVPPKRIDT 564
           LTVPGGPSIACSTAKAVEWDAAWSKN+DPSGRAALGVHAS YGDEANG+N +V PK +DT
Sbjct: 541 LTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASTYGDEANGLNAIVQPKIVDT 600

BLAST of HG10004309 vs. ExPASy TrEMBL
Match: A0A6J1J8M0 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC111484448 PE=4 SV=1)

HSP 1 Score: 1073.5 bits (2775), Expect = 2.1e-310
Identity = 519/589 (88.12%), Postives = 539/589 (91.51%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGC+DDS AKRVRVLELSRR  LKHRGPDWSGLYQH DCYL HQRLAIIDPAS
Sbjct: 1   MCGILAVLGCADDSLAKRVRVLELSRR--LKHRGPDWSGLYQHGDCYLTHQRLAIIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNEDKS+VVTVNGEIYNHEELRKKLRNH+ RTGSDCDVISHLYE+YGENFV MLD
Sbjct: 61  GDQPLYNEDKSVVVTVNGEIYNHEELRKKLRNHKFRTGSDCDVISHLYEEYGENFVGMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+AARDAIGITSLYIGWGLDGSVWISSELK LNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           LY+SK+ GFKRWYNPTWFSE IPSTPYDP+VLRRALENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 LYSSKQGGFKRWYNPTWFSEGIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITARHLAGTRAAK WGTQLHSFCVGLEGSPDLKAA+EVADFLGTVHHEFHFTVQD
Sbjct: 241 SLVASITARHLAGTRAAKQWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRESCRK                         PFLDKEF NVAMAIDPEWKM+K
Sbjct: 361 PNKEEFHRESCRKIKELHLYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMVK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            +QG+IEKWVLR+AFDDEQQPYLPK VLYRQKEQFSDGVGYSWIDGLK+HA QHVTDKMM
Sbjct: 421 REQGRIEKWVLRRAFDDEQQPYLPKSVLYRQKEQFSDGVGYSWIDGLKSHAAQHVTDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
            NAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN
Sbjct: 481 QNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVH SAY DEA  VN ++P K IDT+PRMEVS  G+KILS
Sbjct: 541 LDPSGRAALGVHVSAYADEATNVNSILPSKMIDTIPRMEVSTSGLKILS 587

BLAST of HG10004309 vs. ExPASy TrEMBL
Match: A0A6J1G8T6 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita moschata OX=3662 GN=LOC111451773 PE=4 SV=1)

HSP 1 Score: 1069.7 bits (2765), Expect = 4.2e-309
Identity = 516/589 (87.61%), Postives = 541/589 (91.85%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  LKHRGPDWSGLYQH D YL HQRLAIIDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LKHRGPDWSGLYQHTDFYLTHQRLAIIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPL+NEDKSIVVTVNGEIYNHEELRKKL NH+ RTGSDCDVI+HLYE+YGENFVDMLD
Sbjct: 61  GDQPLFNEDKSIVVTVNGEIYNHEELRKKLSNHKFRTGSDCDVIAHLYEEYGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+ ARDAIGITSLYIGWGLDGSVWISSELK LNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
            Y+SKE GFKRWYNPTWFSE IPSTPYDP+VLRR+LENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 FYSSKEGGFKRWYNPTWFSEAIPSTPYDPLVLRRSLENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAA+EVADFLGTVHHEFHFTVQD
Sbjct: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK+LGVKMVISGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKALGVKMVISGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNKEEFHRESCRK                         PFLDKEF + AMAIDPEWKMIK
Sbjct: 361 PNKEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDTAMAIDPEWKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            +QG+IEKWVLR+AFDDEQQPYLPK+VLYRQKEQFSDGVGYSWIDGLKAHA QHVTD+MM
Sbjct: 421 REQGRIEKWVLRRAFDDEQQPYLPKNVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDRMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
           LNA HIFPQNTPTSKEAYYYRTIFERFFPQN+A+LT+PGGPSIACSTAKAVEWDAAWSKN
Sbjct: 481 LNAGHIFPQNTPTSKEAYYYRTIFERFFPQNAAQLTIPGGPSIACSTAKAVEWDAAWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRMEVSAPGVKILS 565
           +DPSGRAALGVH SAYGDEA GV  VVPP  ++T+PRMEVSAPGV+ILS
Sbjct: 541 LDPSGRAALGVHVSAYGDEATGVKSVVPPPIVNTIPRMEVSAPGVEILS 587

BLAST of HG10004309 vs. TAIR 10
Match: AT3G47340.1 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 959.1 bits (2478), Expect = 1.5e-279
Identity = 456/578 (78.89%), Postives = 508/578 (87.89%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGCSDDSQAKRVRVLELSRR  L+HRGPDWSGLYQ+ D YLAHQRLA+IDPAS
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRR--LRHRGPDWSGLYQNGDNYLAHQRLAVIDPAS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPL+NEDK+IVVTVNGEIYNHEELRK+L+NH+ RTGSDC+VI+HLYE+YG +FVDMLD
Sbjct: 61  GDQPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           G+FSFVLLDTRDNSFM ARDAIG+TSLYIGWGLDGSVWISSE+K LNDDCEHFETFPPGH
Sbjct: 121 GIFSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
            Y+SK  GFK+WYNP WF+E +PSTPY+P+ +RRA ENAVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 FYSSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVASITARHLAGT+AAK WG QLHSFCVGLEGSPDLKA KEVA++LGTVHHEFHF+VQD
Sbjct: 241 SLVASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIEDVIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEGADEIFGGYLYFHKA
Sbjct: 301 GIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNK+EFH+E+CRK                         PFLDK+F N AM++DPE KMIK
Sbjct: 361 PNKKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESKMIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            ++G+IEKWVLR+AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA Q+V DKMM
Sbjct: 421 PEEGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVNDKMM 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
            NA HIFP NTP +KEAYYYR IFERFFPQNSARLTVPGG ++ACSTAKAVEWDA+WS N
Sbjct: 481 SNAGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDASWSNN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPRM 554
           +DPSGRAA+GVH SAY D  N    + P K ID +P M
Sbjct: 541 MDPSGRAAIGVHLSAY-DGKNVALTIPPLKAIDNMPMM 575

BLAST of HG10004309 vs. TAIR 10
Match: AT5G65010.1 (asparagine synthetase 2 )

HSP 1 Score: 899.8 bits (2324), Expect = 1.1e-261
Identity = 417/577 (72.27%), Postives = 490/577 (84.92%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGC D+SQAKR R++ELSRR  L+HRGPDWSGL+ + DCYLAH+RLAIIDP S
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRR--LRHRGPDWSGLHCYEDCYLAHERLAIIDPTS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNEDK++ VTVNGEIYNH+ LR+KL++H+ RTGSDC+VI+HLYE++GE F+DMLD
Sbjct: 61  GDQPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMF+FVLLDTRD SF+AARDAIGIT LYIGWGLDGSVW +SE+KAL+DDCE F +FPPGH
Sbjct: 121 GMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           +Y+SK+ G +RWYNP W++E +PSTPYDP+VLR A E AVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 IYSSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVA++  RHL  + AA+ WG+QLH+FC+GL+GSPDLKA +EVAD+LGT HHEF FTVQD
Sbjct: 241 SLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIE+VIYHIETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEI GGYLYFHKA
Sbjct: 301 GIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNK+EFH E+CRK                         PFLDKEF NVAM+IDPEWK+IK
Sbjct: 361 PNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            D G+IEKWVLR AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA++HV+D M+
Sbjct: 421 PDLGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSDTML 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 540
            NA  +FP NTP +KEAYYYRTIFE+FFP+++AR TVPGGPSIACSTAKAVEWDA WSKN
Sbjct: 481 SNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSIACSTAKAVEWDATWSKN 540

Query: 541 IDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPR 553
           +DPSGRAALGVH +AY ++            +D +P+
Sbjct: 541 LDPSGRAALGVHVAAYEEDKAAAAAKAGSDLVDPLPK 575

BLAST of HG10004309 vs. TAIR 10
Match: AT5G10240.1 (asparagine synthetase 3 )

HSP 1 Score: 898.7 bits (2321), Expect = 2.5e-261
Identity = 415/559 (74.24%), Postives = 484/559 (86.58%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGC D+SQAKR R++ELSRR  L+HRGPDWSGL+ + DCYLAH+RLAI+DP S
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRR--LRHRGPDWSGLHCYEDCYLAHERLAIVDPTS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNEDK+I VTVNGEIYNH+ LR+ L++H+ RTGSDC+VI+HLYE++GE FVDMLD
Sbjct: 61  GDQPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMF+FVLLDTRD SF+AARDAIGIT LYIGWGLDGSVW +SE+KAL+DDCE F  FPPGH
Sbjct: 121 GMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           +Y+SK+ G +RWYNP WFSE +PSTPYDP+V+R   E AVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 IYSSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVAS+  RHL  + AA  WG++LH+FC+GL+GSPDLKA +EVAD+LGT HHE HFTVQD
Sbjct: 241 SLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIE+VIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEIFGGYLYFHKA
Sbjct: 301 GIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNK+EFH E+CRK                         PFLDKEF NVAM+IDPEWKMI+
Sbjct: 361 PNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIR 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            D G+IEKWVLR AFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA++HV++ M+
Sbjct: 421 PDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSETML 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 535
           +NA  +FP NTP +KEAYYYRTIFE+FFP+++AR TVPGGPS+ACSTAKAVEWDAAWS+N
Sbjct: 481 MNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAWSQN 540

BLAST of HG10004309 vs. TAIR 10
Match: AT5G65010.2 (asparagine synthetase 2 )

HSP 1 Score: 896.7 bits (2316), Expect = 9.3e-261
Identity = 418/578 (72.32%), Postives = 490/578 (84.78%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGC D+SQAKR R++ELSRR  L+HRGPDWSGL+ + DCYLAH+RLAIIDP S
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRR--LRHRGPDWSGLHCYEDCYLAHERLAIIDPTS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNEDK++ VTVNGEIYNH+ LR+KL++H+ RTGSDC+VI+HLYE++GE F+DMLD
Sbjct: 61  GDQPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMF+FVLLDTRD SF+AARDAIGIT LYIGWGLDGSVW +SE+KAL+DDCE F +FPPGH
Sbjct: 121 GMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           +Y+SK+ G +RWYNP W++E +PSTPYDP+VLR A E AVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 IYSSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVA++  RHL  + AA+ WG+QLH+FC+GL+GSPDLKA +EVAD+LGT HHEF FTVQD
Sbjct: 241 SLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIE+VIYHIETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEI GGYLYFHKA
Sbjct: 301 GIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNK+EFH E+CRK                         PFLDKEF NVAM+IDPEWK+IK
Sbjct: 361 PNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIK 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            D G+IEKWVLR AFDDE++PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA++HV+D M+
Sbjct: 421 PDLGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSDTML 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFP-QNSARLTVPGGPSIACSTAKAVEWDAAWSK 540
            NA  +FP NTP +KEAYYYRTIFE+FFP Q++AR TVPGGPSIACSTAKAVEWDA WSK
Sbjct: 481 SNASFVFPDNTPLTKEAYYYRTIFEKFFPKQSAARATVPGGPSIACSTAKAVEWDATWSK 540

Query: 541 NIDPSGRAALGVHASAYGDEANGVNEVVPPKRIDTVPR 553
           N+DPSGRAALGVH +AY ++            +D +P+
Sbjct: 541 NLDPSGRAALGVHVAAYEEDKAAAAAKAGSDLVDPLPK 576

BLAST of HG10004309 vs. TAIR 10
Match: AT5G10240.2 (asparagine synthetase 3 )

HSP 1 Score: 888.3 bits (2294), Expect = 3.3e-258
Identity = 413/559 (73.88%), Postives = 482/559 (86.23%), Query Frame = 0

Query: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHADCYLAHQRLAIIDPAS 60
           MCGILAVLGC D+SQAKR R++ELSRR  L+HRGPDWSGL+ + DCYLAH+RLAI+DP S
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRR--LRHRGPDWSGLHCYEDCYLAHERLAIVDPTS 60

Query: 61  GDQPLYNEDKSIVVTVNGEIYNHEELRKKLRNHRIRTGSDCDVISHLYEQYGENFVDMLD 120
           GDQPLYNEDK+I VTVNGEIYNH+ LR+ L++H+ RTGSDC+VI+HL  ++GE FVDMLD
Sbjct: 61  GDQPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLV-KHGEEFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWGLDGSVWISSELKALNDDCEHFETFPPGH 180
           GMF+FVLLDTRD SF+AARDAIGIT LYIGWGLDGSVW +SE+KAL+DDCE F  FPPGH
Sbjct: 121 GMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGH 180

Query: 181 LYTSKEAGFKRWYNPTWFSEDIPSTPYDPIVLRRALENAVIKRLITDVPFGVLLSGGLDS 240
           +Y+SK+ G +RWYNP WFSE +PSTPYDP+V+R   E AVIKRL+TDVPFGVLLSGGLDS
Sbjct: 181 IYSSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 SLVASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAKEVADFLGTVHHEFHFTVQD 300
           SLVAS+  RHL  + AA  WG++LH+FC+GL+GSPDLKA +EVAD+LGT HHE HFTVQD
Sbjct: 241 SLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQD 300

Query: 301 GIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKA 360
           GIDAIE+VIYH+ETYDVTTIRASTPMFLM+RKIKSLGVKMV+SGEG+DEIFGGYLYFHKA
Sbjct: 301 GIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKA 360

Query: 361 PNKEEFHRESCRK-------------------------PFLDKEFFNVAMAIDPEWKMIK 420
           PNK+EFH E+CRK                         PFLDKEF NVAM+IDPEWKMI+
Sbjct: 361 PNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIR 420

Query: 421 HDQGQIEKWVLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMM 480
            D G+IEKWVLR AFDDE+ PYLPKH+LYRQKEQFSDGVGYSWIDGLK HA++HV++ M+
Sbjct: 421 PDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSETML 480

Query: 481 LNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKN 535
           +NA  +FP NTP +KEAYYYRTIFE+FFP+++AR TVPGGPS+ACSTAKAVEWDAAWS+N
Sbjct: 481 MNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAWSQN 540