HG10004285 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10004285 vs. NCBI nr
Match: XP_022141775.1 (TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 1598.9 bits (4139), Expect = 0.0e+00
Identity = 870/1411 (61.66%), Postives = 1026/1411 (72.71%), Query Frame = 0

Query: 71   EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
            EVD++K+IT QIF+R RPK+ TSDKNLVGM   L +MNV+LGLGLDDVRF+G+VGMGGIG
Sbjct: 202  EVDVVKKITNQIFERWRPKIATSDKNLVGMTRQLLEMNVKLGLGLDDVRFVGIVGMGGIG 261

Query: 131  KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
            KTTIAKVVYDCI SKF+GSCFL + G  +SK+ NL+SLQEQLLS   LKEN+RIWDE+ G
Sbjct: 262  KTTIAKVVYDCITSKFEGSCFLRVFGG-SSKQCNLVSLQEQLLSRLFLKENVRIWDEDYG 321

Query: 191  AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKILE 250
            AEMI N+L ++KVLLVLD VE+KEQL +LA  P WFGPGSR+IITTRNK+IL QPY+ILE
Sbjct: 322  AEMIENQLCSRKVLLVLDEVEEKEQLEMLARSPDWFGPGSRIIITTRNKEILHQPYEILE 381

Query: 251  FNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYLNKSWV- 310
            + +K LD  SA QLF N AFGIDYI+ +F+NLSK +V+KLGRLP+ALKVIGSYLNK  V 
Sbjct: 382  YKMKLLDDDSALQLFCNRAFGIDYIDSSFMNLSKGMVEKLGRLPLALKVIGSYLNKKDVL 441

Query: 311  IWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFGY 370
            IWKETLNRL+KVDEK+LFEVL ISYDGLGAE+QKVFLDLACFFNG++ DKVIK+L+SFGY
Sbjct: 442  IWKETLNRLIKVDEKHLFEVLNISYDGLGAESQKVFLDLACFFNGRRTDKVIKILESFGY 501

Query: 371  NPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCAF 430
            NP+S+L L  E+CL+EVS +KILMH+++ ALGQEIVR+ES+TQ GKQSRIWLQEDVV AF
Sbjct: 502  NPHSELNLFTEKCLIEVSHSKILMHDLIVALGQEIVRKESLTQLGKQSRIWLQEDVVRAF 561

Query: 431  TVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHNVRLSGRIKFLSNLLGF 490
            T+KH GL+YTEGIVLN +KKQKE  LEAKLF  M  LRILEI NV+L G IK LSNLL  
Sbjct: 562  TMKH-GLKYTEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLYGNIKSLSNLLAL 621

Query: 491  LSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYLLETPDF 550
            L+WPGYPSK LPS F S  L+ELHL HSSI  +W GKKEFE LKVIDV DSEYLLETP+F
Sbjct: 622  LNWPGYPSKCLPSNFQSGYLLELHLRHSSIVRIWDGKKEFENLKVIDVSDSEYLLETPNF 681

Query: 551  SMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLECLILSK 610
            S VP LE LVLCNCR L+HIHPSINSL  LKFL+L EC SLKSFSSNLRCKNLE L+LSK
Sbjct: 682  SKVPKLERLVLCNCRRLNHIHPSINSLHLLKFLDLTECSSLKSFSSNLRCKNLERLVLSK 741

Query: 611  SGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSLPNEVGNL 670
            SG+TS PEIE +M +L  L LD T I  LH SI  LVGLI L+L  C MLSSLPNE+GNL
Sbjct: 742  SGVTSSPEIEENMERLCELYLDGTPIKQLHPSIGRLVGLIKLDLRKCIMLSSLPNEIGNL 801

Query: 671  RSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTI---KNLEKLICEGLSP 730
             SL +LLLGGC+NLD++PPSLGN + L+YL+I +TSI  +PPTI   KNL+ L  +GLS 
Sbjct: 802  ESLQSLLLGGCINLDQMPPSLGNVQRLQYLEICETSICHLPPTIHSLKNLKGLKFDGLSG 861

Query: 731  IVWHSLLPPCDIL----------------------------------------------- 790
             +WHSLLPP +IL                                               
Sbjct: 862  RIWHSLLPPSNILESDLGISGSKFGMESLEYLSLRGCNLVEEDIPEDLHYFSSLKTLDLS 921

Query: 791  ---------------------------------------------------------RHN 850
                                                                      H+
Sbjct: 922  GNNFVRLPESINHLKNLRELRLHDCFKLQHLPELRTNLVPIISCTESGAVRNLPIPYHHD 981

Query: 851  KPFVLEFITETDFKLDLKAYEESIYTKGDMFYSTNDNVEVLECFDEIREGNLVEVPYYFP 910
            K F+LEFI +T+ KLD KA+EESI ++G +F ST+DN EVLECFDEIREGN++E+  Y  
Sbjct: 982  KHFILEFIPKTNLKLDPKAFEESI-SEGALFSSTDDNAEVLECFDEIREGNVIEIERYQI 1041

Query: 911  TNDEKCPYGVVLSACIQHSKNSVHKEVTTFYCKFLIEMRFEGNLNTLTCPWTSKLMSRTR 970
             ND    YGVVLSACIQ S+N+ H EV +FYCKFLIE+  E +  T   P TSKL SRTR
Sbjct: 1042 KNDANNIYGVVLSACIQCSQNTAH-EVISFYCKFLIELGMEEHPRTGILPLTSKLTSRTR 1101

Query: 971  FWMLFIPFHMLPKQRVLCFKITVIGTVPAFNIQRCGASVLFHQKAPAFLANIFNRM--RF 1030
            FWMLFIP ++LPK R L FKI   G+    NIQ+CGASVL HQ AP  LAN+FN++    
Sbjct: 1102 FWMLFIPLNLLPKSR-LRFKILAKGSPNTINIQKCGASVLSHQNAPELLANMFNKVYRTR 1161

Query: 1031 NPNKFYIATRGCNHGSECSCDEPETAEQCCSTQFVENESDSFSLLKRNFKTILQRTYEEE 1090
            N ++F    R CNHGS+CSCDEPET +Q CSTQ+VENES  FSLLKR+ K+IL+RTYEEE
Sbjct: 1162 NQDEFCTTIRECNHGSKCSCDEPETIDQYCSTQYVENESGPFSLLKRSLKSILRRTYEEE 1221

Query: 1091 KHCSLSYFFPQGQFPSWFRI-QPKNTVSVKLPFNLFREKKWKGLAVFAIFSI---YEFVH 1150
            K  SL YFFP+G   SWFRI QPK+TV+VKLP +LF+EKKW GLAVFAIFS+    E+VH
Sbjct: 1222 KDHSLYYFFPRGPALSWFRIQQPKDTVAVKLPLDLFKEKKWMGLAVFAIFSVPGNSEYVH 1281

Query: 1151 NHSFKFQIGPDEDVRQLMKGGSYSSDSIFIP-SSLQFSHRILFVRFVPRCMFPYEQGELS 1210
            NH+F FQI  +ED R L+KGGSYSS S  IP S LQ S +ILFV F PR +FPY Q  L+
Sbjct: 1282 NHTFNFQITSNEDGRHLIKGGSYSSGSFLIPLSLLQSSPQILFVTFEPRHVFPYVQEGLN 1341

Query: 1211 HFYVSFITNASRLGVESCGARLVYLHNVEGLLQTIMDCVLKSGQELYQDYTQAILRDGLR 1270
            HFYVSFI+  +RL VESCGARLVY HNVEGL+ TIMDCV KS +ELYQD++Q I+RD L 
Sbjct: 1342 HFYVSFISIPTRLQVESCGARLVYQHNVEGLINTIMDCVPKSREELYQDHSQEIMRDFLT 1401

Query: 1271 TYHQPTWIPVSADPQTTSSSDSSDYYSTKKLPHEGFQHLHPMKSTQDRYK-SQFDVIIH- 1330
            TYH+P  I V AD  TTS+  SSD    +    E FQH   M+STQD+Y  S+ + IIH 
Sbjct: 1402 TYHRPIRIQVPADQLTTST--SSDLSGRR----ESFQHSLFMESTQDQYNISKLEAIIHG 1461

Query: 1331 RRIPDFLTSQSRGNEGIVRIPEELCFLEKKVGVLVCTVVTINKKLAELERISEPWSRVKV 1365
            + IPDF TSQS GN+G VR PE L F + +VGVLVC VV IN +  +LE        VKV
Sbjct: 1462 KEIPDFFTSQSMGNKGAVRTPERLYFAKCEVGVLVCAVVVINNQTEKLE-------HVKV 1521

BLAST of HG10004285 vs. NCBI nr
Match: XP_022141862.1 (TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 1500.3 bits (3883), Expect = 0.0e+00
Identity = 827/1417 (58.36%), Postives = 983/1417 (69.37%), Query Frame = 0

Query: 71   EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
            EV+I+K+IT QIF+R RPK+ TSDKNLVGM Y L +MNV+LGLGLDDVRF+G+VGMGGIG
Sbjct: 217  EVNIVKKITNQIFERWRPKIATSDKNLVGMTYQLLKMNVKLGLGLDDVRFVGIVGMGGIG 276

Query: 131  KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
            KTTIAKVVYDCI S F+GSCFL + G  +SK+ NLLSLQEQLLS   LKEN+R+WDE+ G
Sbjct: 277  KTTIAKVVYDCIKSNFEGSCFLRVFGG-SSKQNNLLSLQEQLLSRLFLKENVRMWDEDYG 336

Query: 191  AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKILE 250
            AEMI N+L ++KVLLVLDGVE+KEQL +LA  P WFGPGSR+IITTRNK+IL QPY+I+E
Sbjct: 337  AEMIKNQLCSRKVLLVLDGVEEKEQLEMLARSPDWFGPGSRIIITTRNKEILHQPYEIIE 396

Query: 251  FNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYLN-KSWV 310
            + +K LDA SA QLF N AFGIDYI+ NF+NLSKEI++KLGRLP+ALKVIGSYLN K  V
Sbjct: 397  YKLKLLDADSALQLFCNRAFGIDYIDSNFMNLSKEILEKLGRLPLALKVIGSYLNKKEEV 456

Query: 311  IWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFGY 370
            IWKETL RL+KVDEKNLFEVLKISYDGLG E+QKVFLDLAC F+G+K+DKVI++L+ FGY
Sbjct: 457  IWKETLYRLIKVDEKNLFEVLKISYDGLGVESQKVFLDLACLFHGRKKDKVIEILECFGY 516

Query: 371  NPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCAF 430
            +PYS+LQL MERCLLEVS TKILMH++V ALGQEIV RES+TQPGKQSRIW QEDV   F
Sbjct: 517  SPYSELQLFMERCLLEVSPTKILMHDLVVALGQEIVHRESLTQPGKQSRIWFQEDVDRVF 576

Query: 431  TVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHNVRLSGRIKFLSNLLGF 490
            TVKH GL+YTEGIVLN +KKQKE  LEAK F  M  LRILEI NV+LSG I FLSNLLGF
Sbjct: 577  TVKH-GLKYTEGIVLNLEKKQKELILEAKSFTDMTYLRILEISNVQLSGNINFLSNLLGF 636

Query: 491  LSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYLLETPDF 550
            ++WPGYPSK LP  F S CL+ELHLP SSIE+LW+GKK+FEKLKVIDV DS+YLL TPDF
Sbjct: 637  INWPGYPSKCLPPNFQSRCLLELHLPLSSIEILWEGKKDFEKLKVIDVSDSKYLLGTPDF 696

Query: 551  SMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLECLILSK 610
            S VPNLE LVL NCR L+HIHPSIN L+RL+ L+L  C S KSFSS+LRCK+LE LILS+
Sbjct: 697  SKVPNLERLVLRNCRKLYHIHPSINDLNRLRLLDLSNCSSFKSFSSHLRCKSLEGLILSE 756

Query: 611  SGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSLPNEVGNL 670
            SGLTS P+ E +M  L  L L  T I  LHSSI  L+GL+ L+L NC MLSSLP+E+GNL
Sbjct: 757  SGLTSSPKFEVNMEHLSRLHLVGTPIKQLHSSIGCLIGLVLLDLRNCIMLSSLPSEIGNL 816

Query: 671  RSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTI---KNLEKLICEGLSP 730
            +SL  LLL GC +LD+IPPSLGNA+CL+YLDI KTSI   PPTI   +NL++L CEGLS 
Sbjct: 817  KSLQVLLLEGCKSLDQIPPSLGNAQCLEYLDIGKTSICQAPPTIHYLENLKRLNCEGLSR 876

Query: 731  IVWHSLLPPCDILRHN-------------------------------------------- 790
             +WHSLLP  DI R N                                            
Sbjct: 877  KIWHSLLPR-DIQRSNRQISGLGFGLKSLEHLCLKNCNLVDEDIPEDLHCFSSLESLDLS 936

Query: 791  ------------------------------------------------------------ 850
                                                                        
Sbjct: 937  GNHFVQYPKSINHLVNLKQLMFHDCFKLQQIPKPSINLEPKFSSVESGMPIDQSLPCQLD 996

Query: 851  KPFVLEFITETDFKLDLKAYEESIYTKGDMFYSTNDNVEVLECFDEIREGNLVEVPYYFP 910
            KPF LE+ +E   KLD K YEE I  +   F +T D  E+LECFDEIREGN VEV Y   
Sbjct: 997  KPFRLEYGSEIKSKLDPKTYEELILERA-TFGTTCDKAEILECFDEIREGNSVEVEYNQV 1056

Query: 911  TNDEKCPYGVVLSACIQHSKNSVHKEVTTFYCKFLIEMRFEGNLNTLTCPWTSKLMSRTR 970
             ND    YGVVLSA IQ SKN+ H EV TFYC F IE+  + N        T  L SR R
Sbjct: 1057 KNDANIIYGVVLSASIQCSKNTSHNEVDTFYCNFSIELETDMNPTITVFYGTFALTSRIR 1116

Query: 971  FWMLFIPFHMLPK----QRVLC-FKITVIGTVPAFNIQRCGASVLFHQKAPAFLANIFNR 1030
            FW LFIP H LPK    Q  LC FK T+   V + +IQ CGAS+L HQ A  F+A +F+R
Sbjct: 1117 FWTLFIPSHKLPKSTSMQNSLCSFKATIKYEVSSVHIQMCGASILSHQNASQFMAKMFSR 1176

Query: 1031 MRFNPNKFYIATRGCNHGSECSCDEPETAEQCCSTQFVENESDSFSLLKRNFKTILQRTY 1090
            M     KF     G NH SEC+CDEPET E     QFVENE DSFSLL+RN K+IL+RTY
Sbjct: 1177 MYCIQEKFQTFIVGGNHWSECNCDEPETVEHNYLIQFVENERDSFSLLRRNLKSILRRTY 1236

Query: 1091 EEEKHCSLSYFFPQGQFPSWFRIQPKN-TVSVKLPFNLFREKKWKGLAVFAIFSI---YE 1150
            E+ KH    YFFPQ Q PSWFRIQ  N TV +KLP N+FREKKW GLA+FA+FS+    E
Sbjct: 1237 EDTKHIFPFYFFPQNQVPSWFRIQQLNDTVKMKLPPNMFREKKWMGLAMFAVFSVERNSE 1296

Query: 1151 FVHNHSFKFQIGPDEDVRQLMKGGSYSSDS-IFIPSSLQF-SHRILFVRFVPRCMFPYEQ 1210
            FV +H F +QI  DE+   L +  SY+  S   IP  L + S +ILF+ F PR   PY Q
Sbjct: 1297 FVKDHPFSWQIDIDENGTYLKRVRSYAFHSPHMIPLQLLWSSSKILFMIFKPRETSPYVQ 1356

Query: 1211 GELSHFYVSFITNASRLGVESCGARLVYLHNVEGLLQTIMDCVLKSGQELYQDYTQAILR 1270
             + +H YV F+TN++ L VE CGARLVY HNV+G +  I+DCVLKS Q+  Q+Y+  ILR
Sbjct: 1357 DKSNHLYVKFLTNSTSLKVELCGARLVYQHNVDGFIHKIVDCVLKSEQKQCQNYSHKILR 1416

Query: 1271 DGLRTYHQPTWIPVSADPQTTSSSDSSDYYSTKKLPHEGFQHLHPMKSTQDRYKSQFDVI 1330
            D L TY QP W  V+    T++S++ + + ST+ L HE  Q     +  QDRYKS+F+ I
Sbjct: 1417 DLLATYQQPIWKKVA----TSTSNNPTQHLSTETLRHESSQRPPFKERIQDRYKSKFNSI 1476

Query: 1331 IH-RRIPDFLTSQSRGNEGIVRIPEELCFLEKKVGVLVCTVVTINKKLAELERISEPWSR 1368
            IH + IP+F  S+S GNE  +RIPE + F+E  VGVL+C VVTI+K + +LERI EP S 
Sbjct: 1477 IHGKEIPNFFISRSMGNEATIRIPEIVNFIEYHVGVLMCAVVTISKLIEKLERIPEPQSG 1536

BLAST of HG10004285 vs. NCBI nr
Match: XP_022141776.1 (LOW QUALITY PROTEIN: TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 717/1191 (60.20%), Postives = 844/1191 (70.86%), Query Frame = 0

Query: 105  QQMNVQLGLGLDDVRFIGMVGMGGIGKTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGN 164
            ++MN+QLGLGLDDVRFIG+VGMGGIGKT IA+VVYDCI SKF+ +CFL + G  +S++ N
Sbjct: 161  KKMNMQLGLGLDDVRFIGIVGMGGIGKTKIAEVVYDCIKSKFECNCFLRVFGG-SSRQIN 220

Query: 165  LLSLQEQLLSHFCLKENIRIWDENLGAEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPH 224
            L+SLQEQLLS   LKEN+RIWDE+ GAEMI  +L ++KVLLVLDGVE+KEQL +LA  P 
Sbjct: 221  LVSLQEQLLSRLFLKENVRIWDEDYGAEMIEKQLCSRKVLLVLDGVEEKEQLEMLARSPD 280

Query: 225  WFGPGSRVIITTRNKDILRQPYKILEFNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSK 284
            WFGPGSR+IITTRNK++L QPY+ILE  VK LD  +A QLF NHAFGI +I+ NF+NLSK
Sbjct: 281  WFGPGSRIIITTRNKEVLHQPYEILEHKVKLLDNDNALQLFCNHAFGIAHIDNNFMNLSK 340

Query: 285  EIVDKLGRLPIALKVIGSYLN-KSWVIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQK 344
            E+V+   RLP+ALKVIGSYLN K+ V+WKETLNRL+KV E+ LFEVLKISYDGLG E+QK
Sbjct: 341  EMVEXAWRLPLALKVIGSYLNKKNGVVWKETLNRLIKVKEERLFEVLKISYDGLGVESQK 400

Query: 345  VFLDLACFFNGKKRDKVIKVLDSFGYNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQE 404
            VFLDLACFFNG++ DKVI++L+SFGYNP+S+L L +E+CL+E S +KILMH+++ ALGQE
Sbjct: 401  VFLDLACFFNGRRLDKVIEILESFGYNPHSELHLFIEKCLIEDSHSKILMHDLIVALGQE 460

Query: 405  IVRRESITQPGKQSRIWLQEDVVCAFTVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERM 464
            IVR+ES+TQ GKQSRIWLQEDVV AFT+KH                       AKLF  M
Sbjct: 461  IVRKESLTQLGKQSRIWLQEDVVRAFTIKH-----------------------AKLFADM 520

Query: 465  ASLRILEIHNVRLSGRIKFLSNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLW 524
              LRILEI NV+LSG IK LSNLLG L+WPGYPSK LPS F S  L ELHL HS I  +W
Sbjct: 521  TCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSKCLPSNFQSGYLHELHLRHSGILRIW 580

Query: 525  KGKKEFEKLKVIDVRDSEYLLETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLN 584
             GKKEFE LKVIDV +SEYLLETPDFS VP LE LVL NC  L+HIH SINSL  L  L+
Sbjct: 581  DGKKEFENLKVIDVSNSEYLLETPDFSEVPKLERLVLRNCSRLYHIHHSINSLHHLILLD 640

Query: 585  LQECPSLKSFSSNLRCKNLECLILSKSGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIK 644
            L  C S KSFS+NL CKNLE L+LSKSG+TS PE EG+M  LR L LD+T I  LHSSI+
Sbjct: 641  LTNCSSFKSFSTNLGCKNLEHLVLSKSGVTSSPEFEGNMEHLRKLHLDETPIKQLHSSIE 700

Query: 645  HLVGLICLNLTNCTMLSSLPNEVGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISK 704
            HLVGLI L+L NC MLSSLP+E+GNL SL+ L LGGCVNLD+IPPSLGNA+CL++LDI K
Sbjct: 701  HLVGLILLDLRNCIMLSSLPDEIGNLNSLEALFLGGCVNLDQIPPSLGNAQCLQFLDIGK 760

Query: 705  TSISGVPPTI---KNLEKLICEGLSPIVWHSLLPPCDIL--------------------- 764
            TSI  VPPTI   KNLE L C+GLS  +W SLLPP +IL                     
Sbjct: 761  TSICRVPPTIHCLKNLEGLNCDGLSCSIWRSLLPPDNILGSDLGIPSLKFGLESLEFLSL 820

Query: 765  ------------------------------------------------------------ 824
                                                                        
Sbjct: 821  KDCNLVNEDIPEDLHYFSSLKTLDLSGNNFVWLPESINHLKNLRELKLHDCFKLQQLPQL 880

Query: 825  -----------------------RHNKPFVLEFITETDFKLDLKAYEESIYTKGDMFYST 884
                                    H+K F+LEFI +T+ KLDLKA+EESI ++G +FYST
Sbjct: 881  PTHLEPIISYTESGAIRNLPIPYHHDKHFILEFIPKTNLKLDLKAFEESI-SEGALFYST 940

Query: 885  NDNVEVLECFDEIREGNLVEVPYYFPTNDEKCPYGVVLSACIQHSKNSVHKEVTTFYCKF 944
            +D  EVLECFDE REGN++E+  Y   ND    YGVVL+ACIQ S+N+ H EV TFY KF
Sbjct: 941  DDKAEVLECFDETREGNVIEIDQYQIKNDANSIYGVVLAACIQCSQNTPH-EVITFYRKF 1000

Query: 945  LIEMRFEGNLNTLTCPWTSKLMSRTRFWMLFIPFHMLPKQRVLCFKITVIGTVPA-FNIQ 1004
            L+E+  EGN  TL+ P TSKL S+TRFWM FIP HMLP+ RV CFKI V G++P+  N+Q
Sbjct: 1001 LVELEMEGNPKTLSLPLTSKLTSKTRFWMSFIPSHMLPECRV-CFKIVVKGSLPSVVNVQ 1060

Query: 1005 RCGASVLFHQKAPAFLANIFNRMRF--NPNKFYIATRGCNHGSECSCDEPETAEQCCSTQ 1064
            +CGA VL HQ A  FLA +FN++      NKF    R CNHGS+CSCDEPET  Q CSTQ
Sbjct: 1061 KCGAFVLSHQNASRFLAYMFNKVYHTRKQNKFSTTIRECNHGSKCSCDEPETIYQYCSTQ 1120

Query: 1065 FVENESDSFSLLKRNFKTILQRTYEEEKHCSLSYFFPQGQFPSWFRI-QPKNTVSVKLPF 1124
            +VENESDS SLLKR+ K+ILQRTYEEEK  SL YFFPQG   SWFRI QPK+ V+VKLP 
Sbjct: 1121 YVENESDSLSLLKRSLKSILQRTYEEEKDHSLYYFFPQGPAVSWFRIQQPKDRVAVKLPL 1180

Query: 1125 NLFREKKWKGLAVFAIFSI---YEFVHNHSFKFQIGPDEDVRQLMKGGSYSSDSIFIP-S 1180
            +LFREKKW GLAVFAIFSI    EFVH+H+F FQI  +E+ R LM GGSY SDS  IP S
Sbjct: 1181 DLFREKKWMGLAVFAIFSIPGNSEFVHHHTFNFQITSNENDRHLMSGGSYLSDSFLIPLS 1240

BLAST of HG10004285 vs. NCBI nr
Match: XP_038890520.1 (TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890521.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890522.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890523.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890524.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890525.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890526.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890527.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890528.1 TMV resistance protein N-like isoform X1 [Benincasa hispida] >XP_038890530.1 TMV resistance protein N-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1013.4 bits (2619), Expect = 1.8e-291
Identity = 624/1458 (42.80%), Postives = 841/1458 (57.68%), Query Frame = 0

Query: 71   EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
            EVDI+ +I  QIFD  RPKL   +KNLVGM   L  MN+ LGLGLDDVRF+ +VGMGGIG
Sbjct: 174  EVDIVNKIANQIFDVWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIG 233

Query: 131  KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
            KTTIA+VV+DCI SKFD  CFL + G     K +L+SLQ ++LS    KE+ RIW EN G
Sbjct: 234  KTTIAQVVFDCILSKFDDCCFLTLPG--GDSKQSLVSLQREMLSQIFHKEDFRIWHENHG 293

Query: 191  AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPY--KI 250
             EMI NRL  +KVL+VLDGVE++ QL +LAG   WFGPGSR+IITTRNK IL      ++
Sbjct: 294  VEMIKNRLSGRKVLIVLDGVEERRQLEMLAGSAEWFGPGSRIIITTRNKGILNHHNYDEM 353

Query: 251  LEFNVKFLDAQSAFQLFRNHAFGIDYINR-NFLNLSKEIVDKLGRLPIALKVIGSYL-NK 310
             E+NV+ LD  SA QLF  HAFG ++ N  +F++LS E+++K  RLP+AL+VIGS+L  K
Sbjct: 354  KEYNVEELDHDSALQLFLKHAFGSNHQNNDSFMDLSNEMIEKAKRLPLALRVIGSFLYGK 413

Query: 311  SWVIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDS 370
               IW+ETL RL+KVDE++ F VLKISYDGLG E+Q+VFLD+ CFFNGK  D+VI++L+S
Sbjct: 414  EITIWRETLKRLIKVDERSFFHVLKISYDGLGVESQQVFLDITCFFNGKNEDRVIEILES 473

Query: 371  FGYNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVV 430
            FGY+P S++QLLM+RCL+EVS  KIL+H+++  +G+EIVR+ES+TQP KQSRIWL ED+ 
Sbjct: 474  FGYSPKSEIQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWLHEDLY 533

Query: 431  CAFTVKHVGLEYTEGIVLNFKKKQKE-FRLEAKLFERMASLRILEIHNVRLSGRIKFLSN 490
            C F  KH  L + +GIVL+ +K+ +E   L+A+ F  M  LRILEI+NV LS  I++LS 
Sbjct: 534  CKFAEKH-DLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELSEDIEYLSQ 593

Query: 491  LLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYLLE 550
            LL  ++WPGYPSK LP  F S  L EL LPHS I  LW GKK F KLKVIDV +SE+L  
Sbjct: 594  LLRIINWPGYPSKSLPPMFQSRYLFELLLPHSHILRLWDGKKRFPKLKVIDVSNSEHLRV 653

Query: 551  TPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLECL 610
            TPDFS VPNLE LVL NC  L  IHPSINSL +L  L+L+ C  LK F  N+RCKNL+ L
Sbjct: 654  TPDFSGVPNLERLVLSNCVKLCEIHPSINSLSKLILLDLEGCGDLKHFPPNIRCKNLQTL 713

Query: 611  ILSKSGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSLPNE 670
             LS +GL + PEI G+M  L  L LD++ I+ LH SI HL GL+ L+L++C  LSSLP E
Sbjct: 714  KLSGTGLETSPEIGGNMEHLTHLHLDESNITQLHPSIGHLTGLVLLDLSSCLGLSSLPCE 773

Query: 671  VGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTI----KNLEKLIC 730
            +GNL+SL TLLL  C  LD+IP SL NA+ L+ L IS+TSI+ +P +I    KNLE L C
Sbjct: 774  IGNLKSLKTLLLKYCEKLDQIPKSLANAESLETLCISETSITHLPSSIIHCLKNLETLEC 833

Query: 731  EGLSPIVWHSLLPP---------------------CDILRHNKPFVLEFITE-------- 790
            EGLS  +W S+LP                      C ++  + P  L F +         
Sbjct: 834  EGLSCGIWKSMLPQFNIHQTVSTGLGCLKTLNLMGCKLMDEDIPEDLHFFSSLETLDLSY 893

Query: 791  ------------------------TDFK--------------LDLKAYEESIYTK----- 850
                                    T+ K              +D ++  E  Y K     
Sbjct: 894  NNFTTLPVSLSHLNKLKTLNLNFCTELKDLPKLPDSLQYVGGIDCRSMSEQYYNKILLIP 953

Query: 851  ---GDMFY------STNDNVE----------------------------------VLECF 910
               G   Y      S + NVE                                  +LE F
Sbjct: 954  SSSGHQLYLNFIIRSKDANVECAMNEFQHSIFTRRSFELNIIEEKPSTIIHKDVDMLEWF 1013

Query: 911  DEIREGNLVEVPY------------YFPTNDEKCPYGVVLSACIQHSKNSVHKEVTTFYC 970
             +I EGN + + Y             +   D     GV LS  I+  +N  H  +  F  
Sbjct: 1014 GQINEGNWINIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLDHLALGRFSV 1073

Query: 971  KFLIEMRFEGNLNTLTCPWTSKLMSRTR-FWMLFIPF-----HMLPKQRVLCFKITVIG- 1030
             F I+ +  G     T  +       TR FW+ +IP      H L  QR    K T+   
Sbjct: 1074 SFEIDGKCSGG----TMGYEMSQFKATRFFWVAYIPIWMFIHHSLMVQRCCSIKATISYF 1133

Query: 1031 ----TVPAFNIQRCG-ASVLFHQKAPAFLANIFNRMRFNPNKFYIATRGCN-HGSECSCD 1090
                      I+ CG +S+L       +LA +F +   +   F    R  N H SEC CD
Sbjct: 1134 CDHIDASKVKIKACGVSSLLSWPNVSEYLAKLFAKRYCSKRNFCTMIRQHNDHNSECRCD 1193

Query: 1091 EPETAEQCCSTQFVENESDSFSLLKRNFKTILQRTYEEEKHCSLSYFFPQGQFPSWFRIQ 1150
            E E A+   S+   E++  +F LL+ N + IL   +EE+K   + YFFPQ +F  WF+ Q
Sbjct: 1194 ELEVAKDDFSSYTFESDDSTF-LLRMNLRAILGIMFEEKKRYYMKYFFPQTKFFGWFKNQ 1253

Query: 1151 -PKNTVSVKLPFNLFREKKWKGLAVFAIFSIYEFVHNHSFKFQIGPDEDVRQLMKGGSYS 1210
              K+ V+VK+P N+ ++KKW GLA+F +FSI +    + F+++I   E +         S
Sbjct: 1254 NKKDKVAVKIPVNIDKDKKWMGLAMFVVFSISKKASCYCFEYEIQTKEKI--------IS 1313

Query: 1211 SDSIFIPSSL-QFSHRILFVRFVPRC-MFPYEQ---GELSHFYVSFITNASRLGVESCGA 1270
            +    IP  + ++S++ILF+ F PR   +PY++      +H Y++F TN+ R+ VE CGA
Sbjct: 1314 TQRHSIPKEVAEYSNQILFIVFEPRYNWYPYDELKSSSSNHVYINFNTNSERMRVELCGA 1373

Query: 1271 RLVYLHNVEGLLQTIMDCVLKSGQELYQDYTQAILRDGLR-------TYHQPTWIPVSAD 1330
            RLVY  NVEGL+ TI++C++KSG ELY+ Y Q I+   L+       T   P   PV   
Sbjct: 1374 RLVYQQNVEGLIHTIVNCIVKSGDELYEYYNQQIVEYHLKMINTHWYTISLPRNNPVKNQ 1433

Query: 1331 PQTTSSSDSSDYYSTKKLPHEGFQHLHPMKSTQDRYKSQFDVIIH-RRIPDFLTSQSRGN 1365
              TT+S+  +   S + L H  F H    KS Q+R+ S+FD+++   +IP F TSQSRGN
Sbjct: 1434 KPTTASTCIASSLSVEHLLHGSFPHPFFHKSIQERFGSKFDLLLRGDKIPKFFTSQSRGN 1493

BLAST of HG10004285 vs. NCBI nr
Match: KAA0039330.1 (TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00512.1 TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 1012.7 bits (2617), Expect = 3.0e-291
Identity = 613/1457 (42.07%), Postives = 842/1457 (57.79%), Query Frame = 0

Query: 71   EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
            EVDI+ +I  QIFD  RPKL   +KNLVGM   L  MN+ LGLGLDDVRF+ +VGMGGIG
Sbjct: 174  EVDIVNKIASQIFDAWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIG 233

Query: 131  KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
            KTTIA+VV+DCI SKFD  CFL + G     K +L+SLQ ++LS    KE+ RIW EN G
Sbjct: 234  KTTIAQVVFDCILSKFDDCCFLTLPG--GDSKQSLVSLQREMLSQIFHKEDFRIWHENHG 293

Query: 191  AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPY--KI 250
             EMI NRL  +KVL+VLDGVE++ QL +LAG   WFGPGSR+IITTRNK +L  P   ++
Sbjct: 294  VEMIKNRLSGRKVLIVLDGVEERRQLEMLAGSTDWFGPGSRIIITTRNKGLLCHPNYDEM 353

Query: 251  LEFNVKFLDAQSAFQLFRNHAFGIDYINR-NFLNLSKEIVDKLGRLPIALKVIGSYL-NK 310
             E+NV+ LD  SA QLF  HAFG ++ N+ +F++LS EIV+K  RLP+AL+VIGS L  K
Sbjct: 354  KEYNVEELDHDSALQLFLKHAFGSNHQNKDSFMDLSNEIVEKAKRLPLALRVIGSSLYGK 413

Query: 311  SWVIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDS 370
               IW+ETL RL+KVDE+N F++LKISYDGLG E+Q+VFLD+ CFFNGK  D+V ++L+S
Sbjct: 414  EITIWRETLKRLIKVDERNFFDILKISYDGLGVESQQVFLDITCFFNGKNEDRVNEILES 473

Query: 371  FGYNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVV 430
            FGY+P S+LQLLM+RCL+EVS  KIL+H+++  +G+EIVR+ES+TQP KQSRIWL ED+ 
Sbjct: 474  FGYSPNSELQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWLHEDLY 533

Query: 431  CAFTVKHVGLEYTEGIVLNFKKKQKE-FRLEAKLFERMASLRILEIHNVRLSGRIKFLSN 490
            C F  KH  L + +GIVL+ +K+ +E   L+A+ F  M  LRILEI+NV L   I++LS 
Sbjct: 534  CRFAEKH-DLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELDEDIEYLSP 593

Query: 491  LLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYLLE 550
            LL  ++W GYPSK LP  F S  L EL LPHS +  +W GKK F KLK+IDV +SE+L  
Sbjct: 594  LLRIINWLGYPSKSLPPTFQSRYLFELLLPHSHLLRVWDGKKRFPKLKLIDVSNSEHLRV 653

Query: 551  TPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLECL 610
            TPDFS VPNLE LVLCNC  L  IHPSINSL++L  L+L+ C  LK F +N+RCKNL+ L
Sbjct: 654  TPDFSGVPNLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKNLQTL 713

Query: 611  ILSKSGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSLPNE 670
             LS +GL  FPEI G M  L  L LD + I+ LH SI +L GL+ L+L+ C  LSSLP E
Sbjct: 714  KLSGTGLEIFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGLSSLPFE 773

Query: 671  VGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTI----KNLEKLIC 730
            +GNL+SL TLLL  C  LDKIPPSL NA+ L+ L IS+TSI+ VP +I    KNLE L C
Sbjct: 774  IGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKNLETLDC 833

Query: 731  EGLSPIVWHSLLPPCDI------------------------------------------- 790
            EGLS  +W SLLP  +I                                           
Sbjct: 834  EGLSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLEALDLSY 893

Query: 791  ------------LRHNKPFVLEFITE---------------------------------- 850
                        L+  K   L + TE                                  
Sbjct: 894  NNFTTLPDSLSHLKKLKTLNLNYCTELKDLPKLPESLQYVGGVDCRSMSEQYYNKILLIP 953

Query: 851  -----------------TDFKLDLKAYEESIYTKGDMFYS---------TNDNVEVLECF 910
                              D +  +  ++ SI+T+     S          +D V++ + F
Sbjct: 954  SSSGHQLYLTFIIPSKDADVECAMNEFQHSIFTRRSFEQSIIEEQPSTIVHDTVDMFQWF 1013

Query: 911  DEIREGNLVEVPY------------YFPTNDEKCPYGVVLSACIQHSKNSVHKEVTTFYC 970
             +I EGN   + Y             +   D     GV LS  I+  +N  H  +  F  
Sbjct: 1014 GQINEGNWTNIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLNHLAIGRFLV 1073

Query: 971  KFLIEMRFEGNLNTLTCPWTSKLMSRTRFWMLFIPF-----HMLPKQRVLCFKITVIG-- 1030
             F I+ +  G          S+  +   FW+ +IP      H L  QR    K+T+    
Sbjct: 1074 SFGIDGKCSGGTMNYE---MSQFKAARFFWVAYIPIWMIKDHSLMVQRCCSVKVTISYCC 1133

Query: 1031 ---TVPAFNIQRCG-ASVLFHQKAPAFLANIFNRMRFNPNKFYIATRGCN-HGSECSCDE 1090
                     I+ CG +S+L       +LA +F     +   FY   R  N H +EC CDE
Sbjct: 1134 DHIDASKVKIKACGVSSMLSWPNVAEYLAKLFTERFCSKRNFYTMIRQHNDHQTECRCDE 1193

Query: 1091 PETAEQCCSTQFVENESDSFSLLKRNFKTILQRTYEEEKHCSLSYFFPQGQFPSWFRIQ- 1150
             E  +   S+   E+ +DS  LL++N + IL   +EE+K   + YFFP  +   WF+ Q 
Sbjct: 1194 LEVGKDDFSSSTFES-NDSTFLLRKNLRAILGVMFEEKKRYYMKYFFPHTKIFGWFKNQN 1253

Query: 1151 PKNTVSVKLPFNLFREKKWKGLAVFAIFSIYEFVHNHSFKFQIGPDEDVRQLMKGGSYSS 1210
             K+ V+VK+P N+ +++KW GLA+F +FSI E    + F+++I   E++    +    S+
Sbjct: 1254 KKDKVAVKIPVNIEKDRKWMGLAMFVVFSISEKASCYCFEYEIQTKENIISTQR-HFIST 1313

Query: 1211 DSIFIPSSLQFSHRILFVRFVPRC-MFPYEQ---GELSHFYVSFITNASRLGVESCGARL 1270
            D +     L+ S++ILFV F PR   +PY++      +H Y++F TN +R+ VE CGARL
Sbjct: 1314 DQV-----LEHSNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNTNGARMRVEFCGARL 1373

Query: 1271 VYLHNVEGLLQTIMDCVLKSGQELYQDYTQAILRDGLRTYHQPTWIPVS--------ADP 1330
            VY  NVEGL+ TI++C+ +SG ELY+ Y Q I+   L T+    W  +S          P
Sbjct: 1374 VYQQNVEGLIHTILNCIAESGDELYEHYNQYIVESHL-TFINTHWYTLSFRRNNSVKNQP 1433

Query: 1331 QTTSSSDSSDYYSTKKLPHEGFQHLHPMKSTQDRYKSQFDVIIH-RRIPDFLTSQSRGNE 1365
             T +S+ ++   S + L +  F H    KS Q+R++S+FD+++H  +IP F ++QS GN 
Sbjct: 1434 STAASTCTASSLSVEHLLYGSFPHPFFHKSLQERFRSKFDLLLHGDKIPKFFSNQSGGNM 1493

BLAST of HG10004285 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 3.9e-100
Identity = 248/704 (35.23%), Postives = 368/704 (52.27%), Query Frame = 0

Query: 71  EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
           E + IK+IT  IF + + K +    NLVG+   +++M ++L L   DVR +G+ G+GGIG
Sbjct: 170 ESNQIKEITNSIFRQLKCKRLDVGANLVGIDSHVKEMILRLHLESSDVRMVGIYGVGGIG 229

Query: 131 KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIR-IWDENL 190
           KTTIAKV+Y+ ++ +F+   FL  + +  S    L  LQ QLL      E  + I     
Sbjct: 230 KTTIAKVIYNELSCEFEYMSFLENIRE-GSNPQVLFHLQNQLLGDILEGEGSQNISSVAH 289

Query: 191 GAEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKIL 250
            A MI + L +++V +VLD V+   QL  L G   W G GSRVIITTRNK +L       
Sbjct: 290 RASMIKDILLSRRVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLAVQEVDD 349

Query: 251 EFNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYLNKSWV 310
            + V+ L+ + A +LF  +AF  +    ++ NL+  +V     LP+ALKV+GS L K  +
Sbjct: 350 LYEVEGLNFEEACELFSLYAFKQNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTI 409

Query: 311 -IWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFG 370
             W+  L +L    + ++ +VLK SYDGL    + +FLDLACFF G+ RD V+++LD   
Sbjct: 410 PQWEGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCD 469

Query: 371 YNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCA 430
           +   + +  L + CL+ +   +I MH++++ +G EIVR     +P K SR+W   D   A
Sbjct: 470 FPAETGISNLNDLCLITLPYNQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERA 529

Query: 431 FTVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHN--------------- 490
            T    G++  E + L+   K K     + +F +M  LR+L++++               
Sbjct: 530 LTADE-GIKSVETMSLDL-SKLKRVCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDI 589

Query: 491 -------------VRLSGRIKFLSNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIE 550
                        ++L    KF S  L +L W GYP   LP  F    LVELHL  S+I+
Sbjct: 590 EEVYDVVMKDASKMQLGQSFKFPSYELRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIK 649

Query: 551 MLWKGKKEFEKLKVIDVRDSEYLLETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLK 610
            LW+G K+ E+LKVID+  S  L +  +FS +PNLE L L  C +L  IHPS+ ++ +L 
Sbjct: 650 QLWQGHKDLERLKVIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLT 709

Query: 611 FLNLQECPSLKSFSSNL-RCKNLECLILSK-SGLTSFPEIEGSMTQLRGLVLDKTGISTL 670
            L+L+ C  LK+   ++   ++LE L LS  S    FPE  G+M  L  L L  T I  L
Sbjct: 710 TLSLRSCNKLKNLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDL 769

Query: 671 HSSIKHLVGLICLNLTNC-----------------------TMLSSLPNEVGNLRSLDTL 720
             SI  L  L  L L+NC                       T +  LP+ +G+L SL+ L
Sbjct: 770 PDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLEIL 829

BLAST of HG10004285 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 4.4e-99
Identity = 251/705 (35.60%), Postives = 368/705 (52.20%), Query Frame = 0

Query: 71  EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
           E + IK+IT  IF R + K + +  NLVG+   +++M  +L +   DVR +GM G+GGIG
Sbjct: 175 ESNQIKEITDSIFRRLKCKRLDAGANLVGIDSHVKEMIWRLHMESSDVRMVGMYGVGGIG 234

Query: 131 KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIR-IWDENL 190
           KTTIAKV+Y+ ++ +F+   FL  + +  + +G +  LQ QLL      E  + I     
Sbjct: 235 KTTIAKVIYNELSREFEYMSFLENIREKFNTQG-VSPLQNQLLDDILKGEGSQNINSVAH 294

Query: 191 GAEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKIL 250
           GA MI + L +K V +VLD V+ + QL  L     W G GSRVIITTRNK +L       
Sbjct: 295 GASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQKVDD 354

Query: 251 EFNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYLNKSWV 310
            + VK L+ + A +LF  +AF  +    ++ NLS  +V     LP+ALKV+G  L K  +
Sbjct: 355 LYEVKGLNFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTI 414

Query: 311 -IWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFG 370
             W+  L +L +  E  +  VLK SYDGLG   + +FLD+ACFF G+ RD V K+LD+  
Sbjct: 415 PEWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACD 474

Query: 371 YNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCA 430
           ++    ++ L ++CL+ +   +I MH++++ +G EIVR +   +P K SR+W   D   A
Sbjct: 475 FHAEIGIKNLNDKCLITLQYNRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERA 534

Query: 431 FTVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHN--------------- 490
            T  + G++  E I L+   K K     +  F +M  LR+L++ +               
Sbjct: 535 LTA-YKGIKRVETISLDL-SKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADD 594

Query: 491 --------------VRLSGRIKFLSNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSI 550
                         +RL    KF S  L +L W GYP   LPS F    LVELHL  S+I
Sbjct: 595 KVELYDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNI 654

Query: 551 EMLWKGKKEFEKLKVIDVRDSEYLLETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRL 610
           + L  G K+ E LKVID+  S  L +  +FS +PNLE L L  C +L  IHPS+ ++ +L
Sbjct: 655 KQLRLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKL 714

Query: 611 KFLNLQECPSLKSFSSNL-RCKNLECLILSK-SGLTSFPEIEGSMTQLRGLVLDKTGIST 670
             L+L+ C  LK+   ++   ++LE L L+  S    FPE  G+M  L  L L  T I  
Sbjct: 715 TTLSLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDLQNTAIKD 774

Query: 671 LHSSIKHLVGLICLNLTNC-----------------------TMLSSLPNEVGNLRSLDT 720
           L  SI  L  L  L+L++C                       T +  LP+ + +L SL+ 
Sbjct: 775 LPDSIGDLESLKYLDLSDCSKFEKFPEKGGNMKSLRELDLRNTAIKDLPDSIRDLESLER 834

BLAST of HG10004285 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 3.7e-98
Identity = 232/676 (34.32%), Postives = 361/676 (53.40%), Query Frame = 0

Query: 71  EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
           E   I+QI + IFD+    +  ++++LVG+   +++++  L + L  VR +G+ GMGG+G
Sbjct: 164 ESKCIQQILKDIFDKFCFSISITNRDLVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVG 223

Query: 131 KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
           KTT A+ +++     F+ +CFL  + +   +   LL LQ+ LLS     E +   D    
Sbjct: 224 KTTAARALFNRYYQNFESACFLEDVKE-YLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEM 283

Query: 191 AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKILE 250
             ++  RL +KKVL+VLD V   +QL+ L G   WFG GSR++ITTR+  +L+       
Sbjct: 284 CVILKRRLCSKKVLVVLDDVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNHDVHET 343

Query: 251 FNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYLNKSWV- 310
           + +K L+   A +LF  HAF      + F  L   +VD  G LP+ALKV+GS L K  + 
Sbjct: 344 YEIKVLEKDEAIELFNLHAFKRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLD 403

Query: 311 IWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFGY 370
           +W  T++RL    E  +   LKIS+DGL    + +FLD+ACFF G  +  +  +  + G+
Sbjct: 404 VWISTIDRLKDNPEGEIMATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGF 463

Query: 371 NPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCAF 430
           +P   ++ L+E+ L+ +   KI MH++++ +G++I  +ES        RI+  EDV  A 
Sbjct: 464 HPVLGVKTLVEKSLIFILEDKIQMHDLMQEMGRQIAVQES-----PMRRIYRPEDVKDA- 523

Query: 431 TVKHVGLEYTEGIVL----NFKKKQKEFRLEAKLFERMASLRIL--EIHNVRLSGRIKFL 490
            +  +  E  EG++L     F++ + E+   A+  ++   LRIL  E +N      + +L
Sbjct: 524 CIGDMRKEAIEGLLLTEPEQFEEGELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAYL 583

Query: 491 SNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYL 550
            N L +L W  Y S   PS F  + LV L +  SSI  LW G K    L  +D+     L
Sbjct: 584 PNSLLWLEWRNYSSNSFPSNFEPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKL 643

Query: 551 LETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLE 610
           ++TPDF M+ NLE L+L +C  L  +HPS+  L  L  LN+  C SL+   + ++ + LE
Sbjct: 644 IQTPDFRMITNLERLILSSCDALVEVHPSVGFLKNLILLNMDHCISLERLPAIIQSECLE 703

Query: 611 CLILSKS-GLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSL 670
            L L+    L  FPE+E +MT L+ L L  TGI  L +SI+HL  L  L + +C  L SL
Sbjct: 704 VLDLNYCFNLKMFPEVERNMTHLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSL 763

Query: 671 PNEVGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTIKNLEKL--- 730
           P+ +   R+L    +  C  L  +P   GN+ C + L +   SI  +P +I NL  L   
Sbjct: 764 PSSIWRFRNLK---ISECEKLGSLPEIHGNSNCTRELILKLVSIKELPTSIGNLTSLNFL 823

BLAST of HG10004285 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 8.0e-93
Identity = 231/667 (34.63%), Postives = 355/667 (53.22%), Query Frame = 0

Query: 71  EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
           + D I+QI  QI  +     ++  +N+VG+   L+++   L +G++ VR +G+ GMGG+G
Sbjct: 162 DADCIRQIVDQISSKLCKISLSYLQNIVGIDTHLEKIESLLEIGINGVRIMGIWGMGGVG 221

Query: 131 KTTIAKVVYDCIASK------FDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRI 190
           KTTIA+ ++D +  +      FDG+CFL    D    K  + SLQ  LLS   L+E    
Sbjct: 222 KTTIARAIFDTLLGRMDSSYQFDGACFLK---DIKENKRGMHSLQNALLSEL-LREKANY 281

Query: 191 WDENLGAEMIANRLYTKKVLLVLDGVEKKEQ-LNLLAGRPHWFGPGSRVIITTRNKDILR 250
            +E  G   +A+RL +KKVL+VLD ++ K+  L  LAG   WFG GSR+IITTR+K ++ 
Sbjct: 282 NNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLIE 341

Query: 251 QPYKILEFNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSY 310
           +   I E  V  L    + QLF+ HAFG +  N NF  LS E+V+    LP+ALKV GS 
Sbjct: 342 KNDIIYE--VTALPDHESIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLALKVWGSL 401

Query: 311 L-NKSWVIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIK 370
           L N     WK  +  +       + + LKISYDGL  + Q++FLD+ACF  G+++D +++
Sbjct: 402 LHNLRLTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQ 461

Query: 371 VLDSFGYNPYSKLQLLMERCLLEVSS-TKILMHEMVEALGQEIVRRESITQPGKQSRIWL 430
           +L+S        L++L+++ L+ +S   ++ MH++++ +G+ IV  +    PG++SR+WL
Sbjct: 462 ILESCHIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIVNFQK--DPGERSRLWL 521

Query: 431 QEDVVCAFTVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHNVRLSGRIK 490
            ++V    +  + G    E I ++       F  +A   + M  LR+  +        I 
Sbjct: 522 AKEVEEVMS-NNTGTMAMEAIWVSSYSSTLRFSNQA--VKNMKRLRVFNMGRSSTHYAID 581

Query: 491 FLSNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSE 550
           +L N L       YP +  PS F    LV L L H+S+  LW   K    L+ ID+  S+
Sbjct: 582 YLPNNLRCFVCTNYPWESFPSTFELKMLVHLQLRHNSLRHLWTETKHLPSLRRIDLSWSK 641

Query: 551 YLLETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKN 610
            L  TPDF+ +PNLE + L  C  L  +H S+    ++  L L +C SLK F     C N
Sbjct: 642 RLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFP----CVN 701

Query: 611 LECL----ILSKSGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSI----KHLVGLICLNL 670
           +E L    + S   L   PEI G M     + +  +GI  L SSI     H+  L+  N+
Sbjct: 702 VESLEYLGLRSCDSLEKLPEIYGRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNM 761

Query: 671 TNCTMLSSLPNEVGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTI 721
            N   L +LP+ +  L+SL +L + GC  L+ +P  +G+   L+  D S T I   P +I
Sbjct: 762 KN---LVALPSSICRLKSLVSLSVSGCSKLESLPEEIGDLDNLRVFDASDTLILRPPSSI 810

BLAST of HG10004285 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 2.2e-87
Identity = 220/668 (32.93%), Postives = 359/668 (53.74%), Query Frame = 0

Query: 71  EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGL-GLDDVRFIGMVGMGGI 130
           E  ++ +I    F +      + ++ LVG+   L+ +   L    LD V  IG+VGM GI
Sbjct: 161 EAKLVDEIAVDTFKKLNDLAPSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGI 220

Query: 131 GKTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENL 190
           GKTT+A  +Y  +  +FDGSCFL  + + NS +  L SL ++L S      ++ I     
Sbjct: 221 GKTTLADCLYGRMRGQFDGSCFLTNIRE-NSGRSGLESLLQKLFSTVLNDRDLEIGAPGN 280

Query: 191 GAEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKIL 250
             E    RL +K++L+VLD V  ++Q+  L G   W+  GSR+IITTR+  ++ +  K  
Sbjct: 281 AHERFERRLKSKRLLIVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLI-ETIKGR 340

Query: 251 EFNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYL-NKSW 310
           ++ +  L+ + A +LF  +AF   +  + F  L+  ++D     P+ALKV+GS L  +  
Sbjct: 341 KYVLPKLNDREALKLFSLNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDD 400

Query: 311 VIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFG 370
           + W+  L+RL      +++EVL+ SY+ L  E + VFLD+ACFF  +  D V  +L+S G
Sbjct: 401 LYWEAKLDRLKSRSHGDIYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHG 460

Query: 371 YNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEI-VRRESI--------TQPGKQS-- 430
            +    ++ L+++CL+ +S  +I MH+M++ + +EI ++ E+I        ++ G Q   
Sbjct: 461 VDVSGVVKDLVDKCLITLSDNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQW 520

Query: 431 --RIWLQEDVVCAFTVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHN-- 490
             R+W  ED +C    + +G +   GI L+   K +  RL AK F+ M +L+ L+I++  
Sbjct: 521 HIRLWDSED-ICDLLTEGLGTDKIRGIFLD-TSKLRAMRLSAKAFQGMYNLKYLKIYDSH 580

Query: 491 ----------VRLSGRIKFLSNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLW 550
                     + L   + FL N L +L W GYP + +P  F    LV+L LPHS +E +W
Sbjct: 581 CSRGCEAEFKLHLRRGLSFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIW 640

Query: 551 KGKKEFEKLKVIDVRDSEYLLETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLN 610
             +K+   LK +D+  S  L +    +   NLE L L  C +L  +  +IN L++L +LN
Sbjct: 641 DDEKDVGMLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLN 700

Query: 611 LQECPSLKSFSSNLRCKNLECLILSK-SGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSI 670
           L++C SL+S    ++ ++L+ LILS  S L  FP I      +  L+LD T I +L  SI
Sbjct: 701 LRDCTSLRSLPKGIKTQSLQTLILSGCSSLKKFPLIS---ENVEVLLLDGTVIKSLPESI 760

Query: 671 KHLVGLICLNLTNCTMLSSLPNEVGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDIS 711
           +    L  LNL NC  L  L +++  L+ L  L+L GC  L+  P    + + L+ L + 
Sbjct: 761 QTFRRLALLNLKNCKKLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMD 820

BLAST of HG10004285 vs. ExPASy TrEMBL
Match: A0A6J1CJ31 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012055 PE=4 SV=1)

HSP 1 Score: 1598.9 bits (4139), Expect = 0.0e+00
Identity = 870/1411 (61.66%), Postives = 1026/1411 (72.71%), Query Frame = 0

Query: 71   EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
            EVD++K+IT QIF+R RPK+ TSDKNLVGM   L +MNV+LGLGLDDVRF+G+VGMGGIG
Sbjct: 202  EVDVVKKITNQIFERWRPKIATSDKNLVGMTRQLLEMNVKLGLGLDDVRFVGIVGMGGIG 261

Query: 131  KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
            KTTIAKVVYDCI SKF+GSCFL + G  +SK+ NL+SLQEQLLS   LKEN+RIWDE+ G
Sbjct: 262  KTTIAKVVYDCITSKFEGSCFLRVFGG-SSKQCNLVSLQEQLLSRLFLKENVRIWDEDYG 321

Query: 191  AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKILE 250
            AEMI N+L ++KVLLVLD VE+KEQL +LA  P WFGPGSR+IITTRNK+IL QPY+ILE
Sbjct: 322  AEMIENQLCSRKVLLVLDEVEEKEQLEMLARSPDWFGPGSRIIITTRNKEILHQPYEILE 381

Query: 251  FNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYLNKSWV- 310
            + +K LD  SA QLF N AFGIDYI+ +F+NLSK +V+KLGRLP+ALKVIGSYLNK  V 
Sbjct: 382  YKMKLLDDDSALQLFCNRAFGIDYIDSSFMNLSKGMVEKLGRLPLALKVIGSYLNKKDVL 441

Query: 311  IWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFGY 370
            IWKETLNRL+KVDEK+LFEVL ISYDGLGAE+QKVFLDLACFFNG++ DKVIK+L+SFGY
Sbjct: 442  IWKETLNRLIKVDEKHLFEVLNISYDGLGAESQKVFLDLACFFNGRRTDKVIKILESFGY 501

Query: 371  NPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCAF 430
            NP+S+L L  E+CL+EVS +KILMH+++ ALGQEIVR+ES+TQ GKQSRIWLQEDVV AF
Sbjct: 502  NPHSELNLFTEKCLIEVSHSKILMHDLIVALGQEIVRKESLTQLGKQSRIWLQEDVVRAF 561

Query: 431  TVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHNVRLSGRIKFLSNLLGF 490
            T+KH GL+YTEGIVLN +KKQKE  LEAKLF  M  LRILEI NV+L G IK LSNLL  
Sbjct: 562  TMKH-GLKYTEGIVLNLEKKQKELTLEAKLFADMTCLRILEISNVQLYGNIKSLSNLLAL 621

Query: 491  LSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYLLETPDF 550
            L+WPGYPSK LPS F S  L+ELHL HSSI  +W GKKEFE LKVIDV DSEYLLETP+F
Sbjct: 622  LNWPGYPSKCLPSNFQSGYLLELHLRHSSIVRIWDGKKEFENLKVIDVSDSEYLLETPNF 681

Query: 551  SMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLECLILSK 610
            S VP LE LVLCNCR L+HIHPSINSL  LKFL+L EC SLKSFSSNLRCKNLE L+LSK
Sbjct: 682  SKVPKLERLVLCNCRRLNHIHPSINSLHLLKFLDLTECSSLKSFSSNLRCKNLERLVLSK 741

Query: 611  SGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSLPNEVGNL 670
            SG+TS PEIE +M +L  L LD T I  LH SI  LVGLI L+L  C MLSSLPNE+GNL
Sbjct: 742  SGVTSSPEIEENMERLCELYLDGTPIKQLHPSIGRLVGLIKLDLRKCIMLSSLPNEIGNL 801

Query: 671  RSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTI---KNLEKLICEGLSP 730
             SL +LLLGGC+NLD++PPSLGN + L+YL+I +TSI  +PPTI   KNL+ L  +GLS 
Sbjct: 802  ESLQSLLLGGCINLDQMPPSLGNVQRLQYLEICETSICHLPPTIHSLKNLKGLKFDGLSG 861

Query: 731  IVWHSLLPPCDIL----------------------------------------------- 790
             +WHSLLPP +IL                                               
Sbjct: 862  RIWHSLLPPSNILESDLGISGSKFGMESLEYLSLRGCNLVEEDIPEDLHYFSSLKTLDLS 921

Query: 791  ---------------------------------------------------------RHN 850
                                                                      H+
Sbjct: 922  GNNFVRLPESINHLKNLRELRLHDCFKLQHLPELRTNLVPIISCTESGAVRNLPIPYHHD 981

Query: 851  KPFVLEFITETDFKLDLKAYEESIYTKGDMFYSTNDNVEVLECFDEIREGNLVEVPYYFP 910
            K F+LEFI +T+ KLD KA+EESI ++G +F ST+DN EVLECFDEIREGN++E+  Y  
Sbjct: 982  KHFILEFIPKTNLKLDPKAFEESI-SEGALFSSTDDNAEVLECFDEIREGNVIEIERYQI 1041

Query: 911  TNDEKCPYGVVLSACIQHSKNSVHKEVTTFYCKFLIEMRFEGNLNTLTCPWTSKLMSRTR 970
             ND    YGVVLSACIQ S+N+ H EV +FYCKFLIE+  E +  T   P TSKL SRTR
Sbjct: 1042 KNDANNIYGVVLSACIQCSQNTAH-EVISFYCKFLIELGMEEHPRTGILPLTSKLTSRTR 1101

Query: 971  FWMLFIPFHMLPKQRVLCFKITVIGTVPAFNIQRCGASVLFHQKAPAFLANIFNRM--RF 1030
            FWMLFIP ++LPK R L FKI   G+    NIQ+CGASVL HQ AP  LAN+FN++    
Sbjct: 1102 FWMLFIPLNLLPKSR-LRFKILAKGSPNTINIQKCGASVLSHQNAPELLANMFNKVYRTR 1161

Query: 1031 NPNKFYIATRGCNHGSECSCDEPETAEQCCSTQFVENESDSFSLLKRNFKTILQRTYEEE 1090
            N ++F    R CNHGS+CSCDEPET +Q CSTQ+VENES  FSLLKR+ K+IL+RTYEEE
Sbjct: 1162 NQDEFCTTIRECNHGSKCSCDEPETIDQYCSTQYVENESGPFSLLKRSLKSILRRTYEEE 1221

Query: 1091 KHCSLSYFFPQGQFPSWFRI-QPKNTVSVKLPFNLFREKKWKGLAVFAIFSI---YEFVH 1150
            K  SL YFFP+G   SWFRI QPK+TV+VKLP +LF+EKKW GLAVFAIFS+    E+VH
Sbjct: 1222 KDHSLYYFFPRGPALSWFRIQQPKDTVAVKLPLDLFKEKKWMGLAVFAIFSVPGNSEYVH 1281

Query: 1151 NHSFKFQIGPDEDVRQLMKGGSYSSDSIFIP-SSLQFSHRILFVRFVPRCMFPYEQGELS 1210
            NH+F FQI  +ED R L+KGGSYSS S  IP S LQ S +ILFV F PR +FPY Q  L+
Sbjct: 1282 NHTFNFQITSNEDGRHLIKGGSYSSGSFLIPLSLLQSSPQILFVTFEPRHVFPYVQEGLN 1341

Query: 1211 HFYVSFITNASRLGVESCGARLVYLHNVEGLLQTIMDCVLKSGQELYQDYTQAILRDGLR 1270
            HFYVSFI+  +RL VESCGARLVY HNVEGL+ TIMDCV KS +ELYQD++Q I+RD L 
Sbjct: 1342 HFYVSFISIPTRLQVESCGARLVYQHNVEGLINTIMDCVPKSREELYQDHSQEIMRDFLT 1401

Query: 1271 TYHQPTWIPVSADPQTTSSSDSSDYYSTKKLPHEGFQHLHPMKSTQDRYK-SQFDVIIH- 1330
            TYH+P  I V AD  TTS+  SSD    +    E FQH   M+STQD+Y  S+ + IIH 
Sbjct: 1402 TYHRPIRIQVPADQLTTST--SSDLSGRR----ESFQHSLFMESTQDQYNISKLEAIIHG 1461

Query: 1331 RRIPDFLTSQSRGNEGIVRIPEELCFLEKKVGVLVCTVVTINKKLAELERISEPWSRVKV 1365
            + IPDF TSQS GN+G VR PE L F + +VGVLVC VV IN +  +LE        VKV
Sbjct: 1462 KEIPDFFTSQSMGNKGAVRTPERLYFAKCEVGVLVCAVVVINNQTEKLE-------HVKV 1521

BLAST of HG10004285 vs. ExPASy TrEMBL
Match: A0A6J1CL15 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012122 PE=4 SV=1)

HSP 1 Score: 1500.3 bits (3883), Expect = 0.0e+00
Identity = 827/1417 (58.36%), Postives = 983/1417 (69.37%), Query Frame = 0

Query: 71   EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
            EV+I+K+IT QIF+R RPK+ TSDKNLVGM Y L +MNV+LGLGLDDVRF+G+VGMGGIG
Sbjct: 217  EVNIVKKITNQIFERWRPKIATSDKNLVGMTYQLLKMNVKLGLGLDDVRFVGIVGMGGIG 276

Query: 131  KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
            KTTIAKVVYDCI S F+GSCFL + G  +SK+ NLLSLQEQLLS   LKEN+R+WDE+ G
Sbjct: 277  KTTIAKVVYDCIKSNFEGSCFLRVFGG-SSKQNNLLSLQEQLLSRLFLKENVRMWDEDYG 336

Query: 191  AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKILE 250
            AEMI N+L ++KVLLVLDGVE+KEQL +LA  P WFGPGSR+IITTRNK+IL QPY+I+E
Sbjct: 337  AEMIKNQLCSRKVLLVLDGVEEKEQLEMLARSPDWFGPGSRIIITTRNKEILHQPYEIIE 396

Query: 251  FNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYLN-KSWV 310
            + +K LDA SA QLF N AFGIDYI+ NF+NLSKEI++KLGRLP+ALKVIGSYLN K  V
Sbjct: 397  YKLKLLDADSALQLFCNRAFGIDYIDSNFMNLSKEILEKLGRLPLALKVIGSYLNKKEEV 456

Query: 311  IWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFGY 370
            IWKETL RL+KVDEKNLFEVLKISYDGLG E+QKVFLDLAC F+G+K+DKVI++L+ FGY
Sbjct: 457  IWKETLYRLIKVDEKNLFEVLKISYDGLGVESQKVFLDLACLFHGRKKDKVIEILECFGY 516

Query: 371  NPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCAF 430
            +PYS+LQL MERCLLEVS TKILMH++V ALGQEIV RES+TQPGKQSRIW QEDV   F
Sbjct: 517  SPYSELQLFMERCLLEVSPTKILMHDLVVALGQEIVHRESLTQPGKQSRIWFQEDVDRVF 576

Query: 431  TVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHNVRLSGRIKFLSNLLGF 490
            TVKH GL+YTEGIVLN +KKQKE  LEAK F  M  LRILEI NV+LSG I FLSNLLGF
Sbjct: 577  TVKH-GLKYTEGIVLNLEKKQKELILEAKSFTDMTYLRILEISNVQLSGNINFLSNLLGF 636

Query: 491  LSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYLLETPDF 550
            ++WPGYPSK LP  F S CL+ELHLP SSIE+LW+GKK+FEKLKVIDV DS+YLL TPDF
Sbjct: 637  INWPGYPSKCLPPNFQSRCLLELHLPLSSIEILWEGKKDFEKLKVIDVSDSKYLLGTPDF 696

Query: 551  SMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLECLILSK 610
            S VPNLE LVL NCR L+HIHPSIN L+RL+ L+L  C S KSFSS+LRCK+LE LILS+
Sbjct: 697  SKVPNLERLVLRNCRKLYHIHPSINDLNRLRLLDLSNCSSFKSFSSHLRCKSLEGLILSE 756

Query: 611  SGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSLPNEVGNL 670
            SGLTS P+ E +M  L  L L  T I  LHSSI  L+GL+ L+L NC MLSSLP+E+GNL
Sbjct: 757  SGLTSSPKFEVNMEHLSRLHLVGTPIKQLHSSIGCLIGLVLLDLRNCIMLSSLPSEIGNL 816

Query: 671  RSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTI---KNLEKLICEGLSP 730
            +SL  LLL GC +LD+IPPSLGNA+CL+YLDI KTSI   PPTI   +NL++L CEGLS 
Sbjct: 817  KSLQVLLLEGCKSLDQIPPSLGNAQCLEYLDIGKTSICQAPPTIHYLENLKRLNCEGLSR 876

Query: 731  IVWHSLLPPCDILRHN-------------------------------------------- 790
             +WHSLLP  DI R N                                            
Sbjct: 877  KIWHSLLPR-DIQRSNRQISGLGFGLKSLEHLCLKNCNLVDEDIPEDLHCFSSLESLDLS 936

Query: 791  ------------------------------------------------------------ 850
                                                                        
Sbjct: 937  GNHFVQYPKSINHLVNLKQLMFHDCFKLQQIPKPSINLEPKFSSVESGMPIDQSLPCQLD 996

Query: 851  KPFVLEFITETDFKLDLKAYEESIYTKGDMFYSTNDNVEVLECFDEIREGNLVEVPYYFP 910
            KPF LE+ +E   KLD K YEE I  +   F +T D  E+LECFDEIREGN VEV Y   
Sbjct: 997  KPFRLEYGSEIKSKLDPKTYEELILERA-TFGTTCDKAEILECFDEIREGNSVEVEYNQV 1056

Query: 911  TNDEKCPYGVVLSACIQHSKNSVHKEVTTFYCKFLIEMRFEGNLNTLTCPWTSKLMSRTR 970
             ND    YGVVLSA IQ SKN+ H EV TFYC F IE+  + N        T  L SR R
Sbjct: 1057 KNDANIIYGVVLSASIQCSKNTSHNEVDTFYCNFSIELETDMNPTITVFYGTFALTSRIR 1116

Query: 971  FWMLFIPFHMLPK----QRVLC-FKITVIGTVPAFNIQRCGASVLFHQKAPAFLANIFNR 1030
            FW LFIP H LPK    Q  LC FK T+   V + +IQ CGAS+L HQ A  F+A +F+R
Sbjct: 1117 FWTLFIPSHKLPKSTSMQNSLCSFKATIKYEVSSVHIQMCGASILSHQNASQFMAKMFSR 1176

Query: 1031 MRFNPNKFYIATRGCNHGSECSCDEPETAEQCCSTQFVENESDSFSLLKRNFKTILQRTY 1090
            M     KF     G NH SEC+CDEPET E     QFVENE DSFSLL+RN K+IL+RTY
Sbjct: 1177 MYCIQEKFQTFIVGGNHWSECNCDEPETVEHNYLIQFVENERDSFSLLRRNLKSILRRTY 1236

Query: 1091 EEEKHCSLSYFFPQGQFPSWFRIQPKN-TVSVKLPFNLFREKKWKGLAVFAIFSI---YE 1150
            E+ KH    YFFPQ Q PSWFRIQ  N TV +KLP N+FREKKW GLA+FA+FS+    E
Sbjct: 1237 EDTKHIFPFYFFPQNQVPSWFRIQQLNDTVKMKLPPNMFREKKWMGLAMFAVFSVERNSE 1296

Query: 1151 FVHNHSFKFQIGPDEDVRQLMKGGSYSSDS-IFIPSSLQF-SHRILFVRFVPRCMFPYEQ 1210
            FV +H F +QI  DE+   L +  SY+  S   IP  L + S +ILF+ F PR   PY Q
Sbjct: 1297 FVKDHPFSWQIDIDENGTYLKRVRSYAFHSPHMIPLQLLWSSSKILFMIFKPRETSPYVQ 1356

Query: 1211 GELSHFYVSFITNASRLGVESCGARLVYLHNVEGLLQTIMDCVLKSGQELYQDYTQAILR 1270
             + +H YV F+TN++ L VE CGARLVY HNV+G +  I+DCVLKS Q+  Q+Y+  ILR
Sbjct: 1357 DKSNHLYVKFLTNSTSLKVELCGARLVYQHNVDGFIHKIVDCVLKSEQKQCQNYSHKILR 1416

Query: 1271 DGLRTYHQPTWIPVSADPQTTSSSDSSDYYSTKKLPHEGFQHLHPMKSTQDRYKSQFDVI 1330
            D L TY QP W  V+    T++S++ + + ST+ L HE  Q     +  QDRYKS+F+ I
Sbjct: 1417 DLLATYQQPIWKKVA----TSTSNNPTQHLSTETLRHESSQRPPFKERIQDRYKSKFNSI 1476

Query: 1331 IH-RRIPDFLTSQSRGNEGIVRIPEELCFLEKKVGVLVCTVVTINKKLAELERISEPWSR 1368
            IH + IP+F  S+S GNE  +RIPE + F+E  VGVL+C VVTI+K + +LERI EP S 
Sbjct: 1477 IHGKEIPNFFISRSMGNEATIRIPEIVNFIEYHVGVLMCAVVTISKLIEKLERIPEPQSG 1536

BLAST of HG10004285 vs. ExPASy TrEMBL
Match: A0A6J1CK95 (LOW QUALITY PROTEIN: TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012057 PE=4 SV=1)

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 717/1191 (60.20%), Postives = 844/1191 (70.86%), Query Frame = 0

Query: 105  QQMNVQLGLGLDDVRFIGMVGMGGIGKTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGN 164
            ++MN+QLGLGLDDVRFIG+VGMGGIGKT IA+VVYDCI SKF+ +CFL + G  +S++ N
Sbjct: 161  KKMNMQLGLGLDDVRFIGIVGMGGIGKTKIAEVVYDCIKSKFECNCFLRVFGG-SSRQIN 220

Query: 165  LLSLQEQLLSHFCLKENIRIWDENLGAEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPH 224
            L+SLQEQLLS   LKEN+RIWDE+ GAEMI  +L ++KVLLVLDGVE+KEQL +LA  P 
Sbjct: 221  LVSLQEQLLSRLFLKENVRIWDEDYGAEMIEKQLCSRKVLLVLDGVEEKEQLEMLARSPD 280

Query: 225  WFGPGSRVIITTRNKDILRQPYKILEFNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSK 284
            WFGPGSR+IITTRNK++L QPY+ILE  VK LD  +A QLF NHAFGI +I+ NF+NLSK
Sbjct: 281  WFGPGSRIIITTRNKEVLHQPYEILEHKVKLLDNDNALQLFCNHAFGIAHIDNNFMNLSK 340

Query: 285  EIVDKLGRLPIALKVIGSYLN-KSWVIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQK 344
            E+V+   RLP+ALKVIGSYLN K+ V+WKETLNRL+KV E+ LFEVLKISYDGLG E+QK
Sbjct: 341  EMVEXAWRLPLALKVIGSYLNKKNGVVWKETLNRLIKVKEERLFEVLKISYDGLGVESQK 400

Query: 345  VFLDLACFFNGKKRDKVIKVLDSFGYNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQE 404
            VFLDLACFFNG++ DKVI++L+SFGYNP+S+L L +E+CL+E S +KILMH+++ ALGQE
Sbjct: 401  VFLDLACFFNGRRLDKVIEILESFGYNPHSELHLFIEKCLIEDSHSKILMHDLIVALGQE 460

Query: 405  IVRRESITQPGKQSRIWLQEDVVCAFTVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERM 464
            IVR+ES+TQ GKQSRIWLQEDVV AFT+KH                       AKLF  M
Sbjct: 461  IVRKESLTQLGKQSRIWLQEDVVRAFTIKH-----------------------AKLFADM 520

Query: 465  ASLRILEIHNVRLSGRIKFLSNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLW 524
              LRILEI NV+LSG IK LSNLLG L+WPGYPSK LPS F S  L ELHL HS I  +W
Sbjct: 521  TCLRILEISNVQLSGNIKSLSNLLGLLNWPGYPSKCLPSNFQSGYLHELHLRHSGILRIW 580

Query: 525  KGKKEFEKLKVIDVRDSEYLLETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLN 584
             GKKEFE LKVIDV +SEYLLETPDFS VP LE LVL NC  L+HIH SINSL  L  L+
Sbjct: 581  DGKKEFENLKVIDVSNSEYLLETPDFSEVPKLERLVLRNCSRLYHIHHSINSLHHLILLD 640

Query: 585  LQECPSLKSFSSNLRCKNLECLILSKSGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIK 644
            L  C S KSFS+NL CKNLE L+LSKSG+TS PE EG+M  LR L LD+T I  LHSSI+
Sbjct: 641  LTNCSSFKSFSTNLGCKNLEHLVLSKSGVTSSPEFEGNMEHLRKLHLDETPIKQLHSSIE 700

Query: 645  HLVGLICLNLTNCTMLSSLPNEVGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISK 704
            HLVGLI L+L NC MLSSLP+E+GNL SL+ L LGGCVNLD+IPPSLGNA+CL++LDI K
Sbjct: 701  HLVGLILLDLRNCIMLSSLPDEIGNLNSLEALFLGGCVNLDQIPPSLGNAQCLQFLDIGK 760

Query: 705  TSISGVPPTI---KNLEKLICEGLSPIVWHSLLPPCDIL--------------------- 764
            TSI  VPPTI   KNLE L C+GLS  +W SLLPP +IL                     
Sbjct: 761  TSICRVPPTIHCLKNLEGLNCDGLSCSIWRSLLPPDNILGSDLGIPSLKFGLESLEFLSL 820

Query: 765  ------------------------------------------------------------ 824
                                                                        
Sbjct: 821  KDCNLVNEDIPEDLHYFSSLKTLDLSGNNFVWLPESINHLKNLRELKLHDCFKLQQLPQL 880

Query: 825  -----------------------RHNKPFVLEFITETDFKLDLKAYEESIYTKGDMFYST 884
                                    H+K F+LEFI +T+ KLDLKA+EESI ++G +FYST
Sbjct: 881  PTHLEPIISYTESGAIRNLPIPYHHDKHFILEFIPKTNLKLDLKAFEESI-SEGALFYST 940

Query: 885  NDNVEVLECFDEIREGNLVEVPYYFPTNDEKCPYGVVLSACIQHSKNSVHKEVTTFYCKF 944
            +D  EVLECFDE REGN++E+  Y   ND    YGVVL+ACIQ S+N+ H EV TFY KF
Sbjct: 941  DDKAEVLECFDETREGNVIEIDQYQIKNDANSIYGVVLAACIQCSQNTPH-EVITFYRKF 1000

Query: 945  LIEMRFEGNLNTLTCPWTSKLMSRTRFWMLFIPFHMLPKQRVLCFKITVIGTVPA-FNIQ 1004
            L+E+  EGN  TL+ P TSKL S+TRFWM FIP HMLP+ RV CFKI V G++P+  N+Q
Sbjct: 1001 LVELEMEGNPKTLSLPLTSKLTSKTRFWMSFIPSHMLPECRV-CFKIVVKGSLPSVVNVQ 1060

Query: 1005 RCGASVLFHQKAPAFLANIFNRMRF--NPNKFYIATRGCNHGSECSCDEPETAEQCCSTQ 1064
            +CGA VL HQ A  FLA +FN++      NKF    R CNHGS+CSCDEPET  Q CSTQ
Sbjct: 1061 KCGAFVLSHQNASRFLAYMFNKVYHTRKQNKFSTTIRECNHGSKCSCDEPETIYQYCSTQ 1120

Query: 1065 FVENESDSFSLLKRNFKTILQRTYEEEKHCSLSYFFPQGQFPSWFRI-QPKNTVSVKLPF 1124
            +VENESDS SLLKR+ K+ILQRTYEEEK  SL YFFPQG   SWFRI QPK+ V+VKLP 
Sbjct: 1121 YVENESDSLSLLKRSLKSILQRTYEEEKDHSLYYFFPQGPAVSWFRIQQPKDRVAVKLPL 1180

Query: 1125 NLFREKKWKGLAVFAIFSI---YEFVHNHSFKFQIGPDEDVRQLMKGGSYSSDSIFIP-S 1180
            +LFREKKW GLAVFAIFSI    EFVH+H+F FQI  +E+ R LM GGSY SDS  IP S
Sbjct: 1181 DLFREKKWMGLAVFAIFSIPGNSEFVHHHTFNFQITSNENDRHLMSGGSYLSDSFLIPLS 1240

BLAST of HG10004285 vs. ExPASy TrEMBL
Match: A0A5A7T7V5 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001220 PE=4 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 1.5e-291
Identity = 613/1457 (42.07%), Postives = 842/1457 (57.79%), Query Frame = 0

Query: 71   EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
            EVDI+ +I  QIFD  RPKL   +KNLVGM   L  MN+ LGLGLDDVRF+ +VGMGGIG
Sbjct: 174  EVDIVNKIASQIFDAWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIG 233

Query: 131  KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
            KTTIA+VV+DCI SKFD  CFL + G     K +L+SLQ ++LS    KE+ RIW EN G
Sbjct: 234  KTTIAQVVFDCILSKFDDCCFLTLPG--GDSKQSLVSLQREMLSQIFHKEDFRIWHENHG 293

Query: 191  AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPY--KI 250
             EMI NRL  +KVL+VLDGVE++ QL +LAG   WFGPGSR+IITTRNK +L  P   ++
Sbjct: 294  VEMIKNRLSGRKVLIVLDGVEERRQLEMLAGSTDWFGPGSRIIITTRNKGLLCHPNYDEM 353

Query: 251  LEFNVKFLDAQSAFQLFRNHAFGIDYINR-NFLNLSKEIVDKLGRLPIALKVIGSYL-NK 310
             E+NV+ LD  SA QLF  HAFG ++ N+ +F++LS EIV+K  RLP+AL+VIGS L  K
Sbjct: 354  KEYNVEELDHDSALQLFLKHAFGSNHQNKDSFMDLSNEIVEKAKRLPLALRVIGSSLYGK 413

Query: 311  SWVIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDS 370
               IW+ETL RL+KVDE+N F++LKISYDGLG E+Q+VFLD+ CFFNGK  D+V ++L+S
Sbjct: 414  EITIWRETLKRLIKVDERNFFDILKISYDGLGVESQQVFLDITCFFNGKNEDRVNEILES 473

Query: 371  FGYNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVV 430
            FGY+P S+LQLLM+RCL+EVS  KIL+H+++  +G+EIVR+ES+TQP KQSRIWL ED+ 
Sbjct: 474  FGYSPNSELQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWLHEDLY 533

Query: 431  CAFTVKHVGLEYTEGIVLNFKKKQKE-FRLEAKLFERMASLRILEIHNVRLSGRIKFLSN 490
            C F  KH  L + +GIVL+ +K+ +E   L+A+ F  M  LRILEI+NV L   I++LS 
Sbjct: 534  CRFAEKH-DLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELDEDIEYLSP 593

Query: 491  LLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYLLE 550
            LL  ++W GYPSK LP  F S  L EL LPHS +  +W GKK F KLK+IDV +SE+L  
Sbjct: 594  LLRIINWLGYPSKSLPPTFQSRYLFELLLPHSHLLRVWDGKKRFPKLKLIDVSNSEHLRV 653

Query: 551  TPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLECL 610
            TPDFS VPNLE LVLCNC  L  IHPSINSL++L  L+L+ C  LK F +N+RCKNL+ L
Sbjct: 654  TPDFSGVPNLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKNLQTL 713

Query: 611  ILSKSGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSLPNE 670
             LS +GL  FPEI G M  L  L LD + I+ LH SI +L GL+ L+L+ C  LSSLP E
Sbjct: 714  KLSGTGLEIFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGLSSLPFE 773

Query: 671  VGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTI----KNLEKLIC 730
            +GNL+SL TLLL  C  LDKIPPSL NA+ L+ L IS+TSI+ VP +I    KNLE L C
Sbjct: 774  IGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKNLETLDC 833

Query: 731  EGLSPIVWHSLLPPCDI------------------------------------------- 790
            EGLS  +W SLLP  +I                                           
Sbjct: 834  EGLSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLEALDLSY 893

Query: 791  ------------LRHNKPFVLEFITE---------------------------------- 850
                        L+  K   L + TE                                  
Sbjct: 894  NNFTTLPDSLSHLKKLKTLNLNYCTELKDLPKLPESLQYVGGVDCRSMSEQYYNKILLIP 953

Query: 851  -----------------TDFKLDLKAYEESIYTKGDMFYS---------TNDNVEVLECF 910
                              D +  +  ++ SI+T+     S          +D V++ + F
Sbjct: 954  SSSGHQLYLTFIIPSKDADVECAMNEFQHSIFTRRSFEQSIIEEQPSTIVHDTVDMFQWF 1013

Query: 911  DEIREGNLVEVPY------------YFPTNDEKCPYGVVLSACIQHSKNSVHKEVTTFYC 970
             +I EGN   + Y             +   D     GV LS  I+  +N  H  +  F  
Sbjct: 1014 GQINEGNWTNIQYEQEFSISKPLNIMYEDVDLSNVCGVFLSTNIEFPQNLNHLAIGRFLV 1073

Query: 971  KFLIEMRFEGNLNTLTCPWTSKLMSRTRFWMLFIPF-----HMLPKQRVLCFKITVIG-- 1030
             F I+ +  G          S+  +   FW+ +IP      H L  QR    K+T+    
Sbjct: 1074 SFGIDGKCSGGTMNYE---MSQFKAARFFWVAYIPIWMIKDHSLMVQRCCSVKVTISYCC 1133

Query: 1031 ---TVPAFNIQRCG-ASVLFHQKAPAFLANIFNRMRFNPNKFYIATRGCN-HGSECSCDE 1090
                     I+ CG +S+L       +LA +F     +   FY   R  N H +EC CDE
Sbjct: 1134 DHIDASKVKIKACGVSSMLSWPNVAEYLAKLFTERFCSKRNFYTMIRQHNDHQTECRCDE 1193

Query: 1091 PETAEQCCSTQFVENESDSFSLLKRNFKTILQRTYEEEKHCSLSYFFPQGQFPSWFRIQ- 1150
             E  +   S+   E+ +DS  LL++N + IL   +EE+K   + YFFP  +   WF+ Q 
Sbjct: 1194 LEVGKDDFSSSTFES-NDSTFLLRKNLRAILGVMFEEKKRYYMKYFFPHTKIFGWFKNQN 1253

Query: 1151 PKNTVSVKLPFNLFREKKWKGLAVFAIFSIYEFVHNHSFKFQIGPDEDVRQLMKGGSYSS 1210
             K+ V+VK+P N+ +++KW GLA+F +FSI E    + F+++I   E++    +    S+
Sbjct: 1254 KKDKVAVKIPVNIEKDRKWMGLAMFVVFSISEKASCYCFEYEIQTKENIISTQR-HFIST 1313

Query: 1211 DSIFIPSSLQFSHRILFVRFVPRC-MFPYEQ---GELSHFYVSFITNASRLGVESCGARL 1270
            D +     L+ S++ILFV F PR   +PY++      +H Y++F TN +R+ VE CGARL
Sbjct: 1314 DQV-----LEHSNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNTNGARMRVEFCGARL 1373

Query: 1271 VYLHNVEGLLQTIMDCVLKSGQELYQDYTQAILRDGLRTYHQPTWIPVS--------ADP 1330
            VY  NVEGL+ TI++C+ +SG ELY+ Y Q I+   L T+    W  +S          P
Sbjct: 1374 VYQQNVEGLIHTILNCIAESGDELYEHYNQYIVESHL-TFINTHWYTLSFRRNNSVKNQP 1433

Query: 1331 QTTSSSDSSDYYSTKKLPHEGFQHLHPMKSTQDRYKSQFDVIIH-RRIPDFLTSQSRGNE 1365
             T +S+ ++   S + L +  F H    KS Q+R++S+FD+++H  +IP F ++QS GN 
Sbjct: 1434 STAASTCTASSLSVEHLLYGSFPHPFFHKSLQERFRSKFDLLLHGDKIPKFFSNQSGGNM 1493

BLAST of HG10004285 vs. ExPASy TrEMBL
Match: A0A1S4E362 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498648 PE=4 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 8.1e-290
Identity = 612/1459 (41.95%), Postives = 840/1459 (57.57%), Query Frame = 0

Query: 71   EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
            EVDI+ +I  QIFD  RPKL   +KNLVGM   L  MN+ LGLGLDDVRF+ +VGMGGIG
Sbjct: 174  EVDIVNKIASQIFDAWRPKLEALNKNLVGMTSRLLHMNMHLGLGLDDVRFVAIVGMGGIG 233

Query: 131  KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
            KTTIA+VV+DCI SKFD  CFL + G     K +L+SLQ ++LS    KE+ +IW EN G
Sbjct: 234  KTTIAQVVFDCILSKFDDCCFLTLPG--GDSKQSLVSLQREMLSQIFHKEDFKIWHENHG 293

Query: 191  AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPY--KI 250
             EMI NRL  +KVL+VLDG E++ QL +LAG   WFGPGSR+IITTRNK +L  P   ++
Sbjct: 294  VEMIKNRLSGRKVLIVLDGAEERRQLEMLAGSTEWFGPGSRIIITTRNKGLLCHPNYDEM 353

Query: 251  LEFNVKFLDAQSAFQLFRNHAFGIDYINR-NFLNLSKEIVDKLGRLPIALKVIGSYL-NK 310
             E+NV+ LD  SA QLF  HAFG ++ N+ +F++LS EIV+K  RLP+AL+VIGS L  K
Sbjct: 354  KEYNVEELDHDSALQLFLKHAFGSNHQNKDSFMDLSNEIVEKAKRLPLALRVIGSSLYGK 413

Query: 311  SWVIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDS 370
               IW+ETL RL+KVDE+N F++LKISYDGLG E+Q+VFLD+ CFFNGK  D+V ++L+S
Sbjct: 414  EITIWRETLKRLIKVDERNFFDILKISYDGLGVESQQVFLDITCFFNGKNEDRVNEILES 473

Query: 371  FGYNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVV 430
            FGY+P S+LQLLM+RCL+EVS  KIL+H+++  +G+EIVR+ES+TQP KQSRIWL ED+ 
Sbjct: 474  FGYSPNSELQLLMQRCLIEVSHKKILVHDLILEMGREIVRKESLTQPEKQSRIWLHEDLY 533

Query: 431  CAFTVKHVGLEYTEGIVLNFKKKQKE-FRLEAKLFERMASLRILEIHNVRLSGRIKFLSN 490
            C F  KH  L + +GIVL+ +K+ +E   L+A+ F  M  LRILEI+NV L   I++LS 
Sbjct: 534  CRFAEKH-DLMHIQGIVLSLEKEMEESIELDAESFSEMTKLRILEINNVELDEDIEYLSP 593

Query: 491  LLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYLLE 550
            LL  ++W GYPSK LP  F S  L EL LPHS +  +W GK+ F KLK+IDV +SE+L  
Sbjct: 594  LLRIINWLGYPSKSLPPTFQSRYLFELLLPHSQLLRVWDGKRRFPKLKLIDVSNSEHLRV 653

Query: 551  TPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLECL 610
            TPDFS VPNLE LVLCNC  L  IHPSINSL++L  L+L+ C  LK F +N+RCKNL+ L
Sbjct: 654  TPDFSGVPNLERLVLCNCVRLCEIHPSINSLNKLILLDLEGCGDLKHFPANIRCKNLQTL 713

Query: 611  ILSKSGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSLPNE 670
             LS +GL  FPEI G M  L  L LD + I+ LH SI +L GL+ L+L+ C  LSSLP E
Sbjct: 714  KLSGTGLEIFPEI-GHMEHLTHLHLDGSKITHLHPSIGYLTGLVFLDLSTCLGLSSLPFE 773

Query: 671  VGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDISKTSISGVPPTI----KNLEKLIC 730
            +GNL+SL TLLL  C  LDKIPPSL NA+ L+ L IS+TSI+ VP +I    KNLE L C
Sbjct: 774  IGNLKSLKTLLLKYCKRLDKIPPSLANAESLETLSISETSITHVPSSIIHCLKNLETLDC 833

Query: 731  EGLSPIVWHSLLPPCDI------------------------------------------- 790
            E LS  +W SLLP  +I                                           
Sbjct: 834  EELSRGIWKSLLPQLNINQTITTGLGCLKALNLMGCKLMDEDIPEDLHCFSSLETLDLSY 893

Query: 791  ------------LRHNKPFVLEFITE---------------------------------- 850
                        L+  K  +L + TE                                  
Sbjct: 894  NNFTTLPDSLSHLKKLKTLILNYCTELKDLPKLPESLQYVGGVDCRSMSEQYYNKILLIP 953

Query: 851  -----------------TDFKLDLKAYEESIYTKGDMFYST---------NDNVEVLECF 910
                              D +  +  ++ SI+T+     S          +D V++ + F
Sbjct: 954  SSSGHQLYLTFIIPSKDADVECVMNEFQHSIFTRRSFEQSIIEKKPSPIFHDTVDMFQWF 1013

Query: 911  DEIREGNLVEVPY-------------YFPTN-DEKCPYGVVLSACIQHSKNSVHKEVTTF 970
             +I EGN   + Y             Y   N    C  GV LS  I+  +N  H  +  F
Sbjct: 1014 GQINEGNWTNIQYEQEFSISKPLNIMYEDVNLSNVC--GVFLSTNIEFPQNLNHLAIGRF 1073

Query: 971  YCKFLIEMRFEGNLNTLTCPWTSKLMSRTRFWMLFIPF-----HMLPKQRVLCFKITVIG 1030
               F I+ +  G          S+  +   FW+ +IP      H L  QR    K+T+  
Sbjct: 1074 LVSFEIDGKCSGGTMNYK---MSQFKAARFFWVAYIPIWMNKDHSLMVQRCCSVKVTISY 1133

Query: 1031 -----TVPAFNIQRCG-ASVLFHQKAPAFLANIFNRMRFNPNKFYIATRGCN-HGSECSC 1090
                       I+ CG +S+L       +LA +F     +   FY   R  N H +EC C
Sbjct: 1134 CCDHIDASKVKIKACGVSSMLSWPNVAEYLAKLFTERFCSKRNFYTMIRQHNDHQTECRC 1193

Query: 1091 DEPETAEQCCSTQFVENESDSFSLLKRNFKTILQRTYEEEKHCSLSYFFPQGQFPSWFRI 1150
            DE E  +   S+   E+ +DS  LL++N + IL   +E +K   + YFFP  +   WF+ 
Sbjct: 1194 DELEVGKDDFSSSTFES-NDSTFLLRKNLRAILGVMFEGKKRYYMKYFFPHTKIFGWFKN 1253

Query: 1151 Q-PKNTVSVKLPFNLFREKKWKGLAVFAIFSIYEFVHNHSFKFQIGPDEDVRQLMKGGSY 1210
            Q  K+ V+VK+P N+ +++KW GLA+F +FSI E    + F+++I   E++       S 
Sbjct: 1254 QNKKDKVAVKIPVNIEKDRKWMGLAMFVVFSISEKASCYCFEYEIQTKENII------ST 1313

Query: 1211 SSDSIFIPSSLQFSHRILFVRFVPRC-MFPYEQ---GELSHFYVSFITNASRLGVESCGA 1270
             S SI     L+ S++ILFV F PR   +PY++      +H Y++F TN +R+ VE CGA
Sbjct: 1314 QSHSISTDQVLEHSNQILFVAFEPRYNWYPYDELKSSSSNHVYINFNTNGARMRVEFCGA 1373

Query: 1271 RLVYLHNVEGLLQTIMDCVLKSGQELYQDYTQAILRDGLRTYHQPTWIPVS--------A 1330
            RLVY  NVEGL+ TI++C+ +SG ELY+ Y Q I+   L T+    W  +S         
Sbjct: 1374 RLVYQQNVEGLVHTILNCIAESGDELYEHYNQYIVESHL-TFINTHWYTLSFRRNNSVKN 1433

Query: 1331 DPQTTSSSDSSDYYSTKKLPHEGFQHLHPMKSTQDRYKSQFDVIIH-RRIPDFLTSQSRG 1365
             P T +S+ ++   S + L +  F H    KS Q+R++S+FD+++H  +IP F ++QS G
Sbjct: 1434 QPSTAASTCTASSLSVEHLLYGSFPHPFFHKSLQERFRSKFDLLLHGDKIPKFFSNQSGG 1493

BLAST of HG10004285 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 371.3 bits (952), Expect = 3.3e-102
Identity = 231/678 (34.07%), Postives = 368/678 (54.28%), Query Frame = 0

Query: 74  IIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIGKTT 133
           +IK+I + I D+         K L+GM   +  +   + +   DVR +G+ GMGG+GKTT
Sbjct: 162 LIKKIVKDISDKLVSTSWDDSKGLIGMSSHMDFLQSMISIVDKDVRMLGIWGMGGVGKTT 221

Query: 134 IAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLGAEM 193
           IAK +Y+ ++ +F   CF+  + +  ++ G +  LQ + L     + +   W       +
Sbjct: 222 IAKYLYNQLSGQFQVHCFMENVKEVCNRYG-VRRLQVEFLCRMFQERDKEAWSSVSCCNI 281

Query: 194 IANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKILEFNV 253
           I  R   K V +VLD V++ EQLN L     WFGPGSR+I+TTR++ +L      L + V
Sbjct: 282 IKERFRHKMVFIVLDDVDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSHGINLVYKV 341

Query: 254 KFLDAQSAFQLFRNHAFGIDYI-NRNFLNLSKEIVDKLGRLPIALKVIGSYL-NKSWVIW 313
           K L  + A QLF N+AF  + I    F  LS + V+    LP+AL+V+GS+L  +S + W
Sbjct: 342 KCLPKKEALQLFCNYAFREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEW 401

Query: 314 KETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFGYNP 373
           + TL RL      ++ EVL++SYDGL  + + +FL ++CF+N K+ D V K+LD  GY  
Sbjct: 402 ESTLARLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAA 461

Query: 374 YSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCAFTV 433
              + +L E+ L+  S+  + +H+++E +G+E+VR++++  P ++  +W  ED +C    
Sbjct: 462 EIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPED-ICHLLS 521

Query: 434 KHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHNVRLSGR--------IKFL 493
           ++ G +  EGI LN  +  + F  + + FE +++L++L  +++   G         + +L
Sbjct: 522 ENSGTQLVEGISLNLSEISEVFASD-RAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSYL 581

Query: 494 SNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLKVIDVRDSEYL 553
              L +L W GYP K +PS+F    LVEL + +S++E LW G +    LK +D+   +YL
Sbjct: 582 PRKLRYLRWDGYPLKTMPSRFFPEFLVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYL 641

Query: 554 LETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSFSSNLRCKNLE 613
           +E PD S   NLE L L  C++L  + PSI +L  L    L  C  LK     +  K+LE
Sbjct: 642 VEVPDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLE 701

Query: 614 CLILSK-SGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSSL 673
            + +S  S L  FPEI  +    R L L  T I  L SSI  L  L+ L++++C  L +L
Sbjct: 702 TVGMSGCSSLKHFPEISWN---TRRLYLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTL 761

Query: 674 PNEVGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLD---------------------I 720
           P+ +G+L SL +L L GC  L+ +P +L N   L+ L+                     I
Sbjct: 762 PSYLGHLVSLKSLNLDGCRRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVSTSIEVLRI 821

BLAST of HG10004285 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 330.5 bits (846), Expect = 6.5e-90
Identity = 235/686 (34.26%), Postives = 348/686 (50.73%), Query Frame = 0

Query: 71  EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
           E + I  ITR+I  R   + +      VG+   LQ ++  L +G D VR I + GMGGIG
Sbjct: 162 EAECIADITREILKRLPCQYLHVPSYAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIG 221

Query: 131 KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
           KTT+AKV ++  +  F+GS FL    + + K      LQ QLLS    + +I     +  
Sbjct: 222 KTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRNDIEFKGLD-- 281

Query: 191 AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKILE 250
              +  R  +K+VLLV+D V+   QLN  A     FG GSR+IITTRN  +L+Q      
Sbjct: 282 -HAVKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEGS 341

Query: 251 FNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYL-NKSWV 310
           ++ K LD   + +LF  HAF      + FL  S+E+V     LP+A++V+G++L  +S  
Sbjct: 342 YSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIR 401

Query: 311 IWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFGY 370
            W+ TL  L ++   N+   L+IS++ L  E + VFLD+ACFF G     V  +LD    
Sbjct: 402 EWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNL 461

Query: 371 NPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCAF 430
            P   L LLMERCL+ +S   I+MH+++  +G++IVR  S  + G++SR+W   DVV   
Sbjct: 462 YPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVV-GV 521

Query: 431 TVKHVGLEYTEGIVLNFKKKQKEFR-LEAKLFERMASLRILEIHNVRLSGRIKFLSNLLG 490
             K  G    EG  L+ K    +F+  E + F +M  LR+LE+  V L+G  +     L 
Sbjct: 522 LKKKSGTNAIEG--LSLKADVMDFQYFEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLR 581

Query: 491 FLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGK---KEFEKLKVIDVRDSEYLLE 550
           +L W G+  +  P   S   L  L L +S+++  WK +   +    +K +D+  S YL E
Sbjct: 582 WLCWHGFSLECFPINLSLESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRE 641

Query: 551 TPDFSMVPNLESLVLCNCRTLHHIHPSINSLD-RLKFLNLQECPSLKSFSSNL-RCKNLE 610
           TPDFS  PN+E L+L NC++L  +H SI  LD +L  LNL  C  L      + + K+LE
Sbjct: 642 TPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLE 701

Query: 611 CLILSK-SGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSS- 670
            L LS  S L    +  G +  L  L+ D T +  + S+I  L  L  L+L  C  L S 
Sbjct: 702 SLFLSNCSKLERLDDALGELESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGLLSD 761

Query: 671 ----------------LPNEVGNLRSLDTLLLGGCVNLDK-IPPSLGNAKCLKYLDISKT 728
                            P  +  L  +  L LG C   D+ IP  +G+   L+ LD+   
Sbjct: 762 DIDNLYSEKSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGN 821

BLAST of HG10004285 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 330.5 bits (846), Expect = 6.5e-90
Identity = 235/686 (34.26%), Postives = 348/686 (50.73%), Query Frame = 0

Query: 71  EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGIG 130
           E + I  ITR+I  R   + +      VG+   LQ ++  L +G D VR I + GMGGIG
Sbjct: 165 EAECIADITREILKRLPCQYLHVPSYAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIG 224

Query: 131 KTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENLG 190
           KTT+AKV ++  +  F+GS FL    + + K      LQ QLLS    + +I     +  
Sbjct: 225 KTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRNDIEFKGLD-- 284

Query: 191 AEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKILE 250
              +  R  +K+VLLV+D V+   QLN  A     FG GSR+IITTRN  +L+Q      
Sbjct: 285 -HAVKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEGS 344

Query: 251 FNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYL-NKSWV 310
           ++ K LD   + +LF  HAF      + FL  S+E+V     LP+A++V+G++L  +S  
Sbjct: 345 YSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIR 404

Query: 311 IWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFGY 370
            W+ TL  L ++   N+   L+IS++ L  E + VFLD+ACFF G     V  +LD    
Sbjct: 405 EWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNL 464

Query: 371 NPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEIVRRESITQPGKQSRIWLQEDVVCAF 430
            P   L LLMERCL+ +S   I+MH+++  +G++IVR  S  + G++SR+W   DVV   
Sbjct: 465 YPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVV-GV 524

Query: 431 TVKHVGLEYTEGIVLNFKKKQKEFR-LEAKLFERMASLRILEIHNVRLSGRIKFLSNLLG 490
             K  G    EG  L+ K    +F+  E + F +M  LR+LE+  V L+G  +     L 
Sbjct: 525 LKKKSGTNAIEG--LSLKADVMDFQYFEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLR 584

Query: 491 FLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGK---KEFEKLKVIDVRDSEYLLE 550
           +L W G+  +  P   S   L  L L +S+++  WK +   +    +K +D+  S YL E
Sbjct: 585 WLCWHGFSLECFPINLSLESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRE 644

Query: 551 TPDFSMVPNLESLVLCNCRTLHHIHPSINSLD-RLKFLNLQECPSLKSFSSNL-RCKNLE 610
           TPDFS  PN+E L+L NC++L  +H SI  LD +L  LNL  C  L      + + K+LE
Sbjct: 645 TPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLE 704

Query: 611 CLILSK-SGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSIKHLVGLICLNLTNCTMLSS- 670
            L LS  S L    +  G +  L  L+ D T +  + S+I  L  L  L+L  C  L S 
Sbjct: 705 SLFLSNCSKLERLDDALGELESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGLLSD 764

Query: 671 ----------------LPNEVGNLRSLDTLLLGGCVNLDK-IPPSLGNAKCLKYLDISKT 728
                            P  +  L  +  L LG C   D+ IP  +G+   L+ LD+   
Sbjct: 765 DIDNLYSEKSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGN 824

BLAST of HG10004285 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 325.9 bits (834), Expect = 1.6e-88
Identity = 220/668 (32.93%), Postives = 359/668 (53.74%), Query Frame = 0

Query: 71  EVDIIKQITRQIFDRCRPKLVTSDKNLVGMMYPLQQMNVQLGL-GLDDVRFIGMVGMGGI 130
           E  ++ +I    F +      + ++ LVG+   L+ +   L    LD V  IG+VGM GI
Sbjct: 161 EAKLVDEIAVDTFKKLNDLAPSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGI 220

Query: 131 GKTTIAKVVYDCIASKFDGSCFLHILGDTNSKKGNLLSLQEQLLSHFCLKENIRIWDENL 190
           GKTT+A  +Y  +  +FDGSCFL  + + NS +  L SL ++L S      ++ I     
Sbjct: 221 GKTTLADCLYGRMRGQFDGSCFLTNIRE-NSGRSGLESLLQKLFSTVLNDRDLEIGAPGN 280

Query: 191 GAEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILRQPYKIL 250
             E    RL +K++L+VLD V  ++Q+  L G   W+  GSR+IITTR+  ++ +  K  
Sbjct: 281 AHERFERRLKSKRLLIVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLI-ETIKGR 340

Query: 251 EFNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSYL-NKSW 310
           ++ +  L+ + A +LF  +AF   +  + F  L+  ++D     P+ALKV+GS L  +  
Sbjct: 341 KYVLPKLNDREALKLFSLNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDD 400

Query: 311 VIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIKVLDSFG 370
           + W+  L+RL      +++EVL+ SY+ L  E + VFLD+ACFF  +  D V  +L+S G
Sbjct: 401 LYWEAKLDRLKSRSHGDIYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHG 460

Query: 371 YNPYSKLQLLMERCLLEVSSTKILMHEMVEALGQEI-VRRESI--------TQPGKQS-- 430
            +    ++ L+++CL+ +S  +I MH+M++ + +EI ++ E+I        ++ G Q   
Sbjct: 461 VDVSGVVKDLVDKCLITLSDNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQW 520

Query: 431 --RIWLQEDVVCAFTVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEIHN-- 490
             R+W  ED +C    + +G +   GI L+   K +  RL AK F+ M +L+ L+I++  
Sbjct: 521 HIRLWDSED-ICDLLTEGLGTDKIRGIFLD-TSKLRAMRLSAKAFQGMYNLKYLKIYDSH 580

Query: 491 ----------VRLSGRIKFLSNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLW 550
                     + L   + FL N L +L W GYP + +P  F    LV+L LPHS +E +W
Sbjct: 581 CSRGCEAEFKLHLRRGLSFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIW 640

Query: 551 KGKKEFEKLKVIDVRDSEYLLETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLN 610
             +K+   LK +D+  S  L +    +   NLE L L  C +L  +  +IN L++L +LN
Sbjct: 641 DDEKDVGMLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLN 700

Query: 611 LQECPSLKSFSSNLRCKNLECLILSK-SGLTSFPEIEGSMTQLRGLVLDKTGISTLHSSI 670
           L++C SL+S    ++ ++L+ LILS  S L  FP I      +  L+LD T I +L  SI
Sbjct: 701 LRDCTSLRSLPKGIKTQSLQTLILSGCSSLKKFPLIS---ENVEVLLLDGTVIKSLPESI 760

Query: 671 KHLVGLICLNLTNCTMLSSLPNEVGNLRSLDTLLLGGCVNLDKIPPSLGNAKCLKYLDIS 711
           +    L  LNL NC  L  L +++  L+ L  L+L GC  L+  P    + + L+ L + 
Sbjct: 761 QTFRRLALLNLKNCKKLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMD 820

BLAST of HG10004285 vs. TAIR 10
Match: AT5G11250.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 320.5 bits (820), Expect = 6.7e-87
Identity = 236/693 (34.05%), Postives = 364/693 (52.53%), Query Frame = 0

Query: 71  EVDIIKQITRQIFDRCRPKLVTSD-KNLVGMMYPLQQMNVQLGLGLDDVRFIGMVGMGGI 130
           E  +IK+I   I +       + D   LVGM   L++M   L L  D+VR IG+ G  GI
Sbjct: 209 EAAMIKKIATDISNILINSTPSRDFDGLVGMRAHLEKMKPLLCLDTDEVRIIGIWGPPGI 268

Query: 131 GKTTIAKVVYDCIASKFDGSCFL-HILGDTNSKKGN-----LLSLQEQLLSHFCLKENIR 190
           GKTTIA+VVY+ ++  F  S F+ +I  +     G+      L LQ+  +S    +++I 
Sbjct: 269 GKTTIARVVYNQLSHSFQLSVFMENIKANYTRPTGSDDYSAKLQLQQMFMSQITKQKDIE 328

Query: 191 IWDENLGAEMIANRLYTKKVLLVLDGVEKKEQLNLLAGRPHWFGPGSRVIITTRNKDILR 250
           I   +LG  +  +RL  KKVL+VLDGV +  QL+ +A    WFGPGSR+IITT+++ + R
Sbjct: 329 I--PHLG--VAQDRLKDKKVLVVLDGVNQSVQLDAMAKEAWWFGPGSRIIITTQDQKLFR 388

Query: 251 QPYKILEFNVKFLDAQSAFQLFRNHAFGIDYINRNFLNLSKEIVDKLGRLPIALKVIGSY 310
                  + V F   + A Q+F  +AFG +     F NL+ ++++  G LP+ L+++GSY
Sbjct: 389 AHGINHIYKVDFPPTEEALQIFCMYAFGQNSPKDGFQNLAWKVINLAGNLPLGLRIMGSY 448

Query: 311 L-NKSWVIWKETLNRLMKVDEKNLFEVLKISYDGLGAETQKVFLDLACFFNGKKRDKVIK 370
               S   WK++L RL    + ++  +LK SYD L  E + +FL +ACFFNGK+    IK
Sbjct: 449 FRGMSREEWKKSLPRLESSLDADIQSILKFSYDALDDEDKNLFLHIACFFNGKE----IK 508

Query: 371 VLDSFGYNPY----SKLQLLMERCLLEVSS-TKILMHEMVEALGQEIVRRESITQPGKQS 430
           +L+      +     +L +L E+ L+  S+   I MH+++  LG EIVR +SI +PG++ 
Sbjct: 509 ILEEHLAKKFVEVRQRLNVLAEKSLISFSNWGTIEMHKLLAKLGGEIVRNQSIHEPGQRQ 568

Query: 431 RIWLQEDVVCAFTVKHVGLEYTEGIVLNFKKKQKEFRLEAKLFERMASLRILEI----HN 490
            ++  E++         G +   GI  ++   ++EF +  ++FE M++L+ L        
Sbjct: 569 FLFDGEEICDVLNGDAAGSKSVIGIDFHY-IIEEEFDMNERVFEGMSNLQFLRFDCDHDT 628

Query: 491 VRLSGRIKFLSNLLGFLSWPGYPSKRLPSKFSSTCLVELHLPHSSIEMLWKGKKEFEKLK 550
           ++LS  + +LS  L  L W  +P   LPS  +   L+EL+L HS ++MLW+G K    L+
Sbjct: 629 LQLSRGLSYLSRKLQLLDWIYFPMTCLPSTVNVEFLIELNLTHSKLDMLWEGVKPLHNLR 688

Query: 551 VIDVRDSEYLLETPDFSMVPNLESLVLCNCRTLHHIHPSINSLDRLKFLNLQECPSLKSF 610
            +D+  S  L E PD S   NL  L+L NC +L  +   I +   L+ L+L  C SL   
Sbjct: 689 QMDLSYSVNLKELPDLSTAINLRKLILSNCSSLIKLPSCIGNAINLEDLDLNGCSSLVEL 748

Query: 611 SSNLRCKNLECLILSK-SGLTSFPEIEGSMTQLRG----------------------LVL 670
            S     NL+ L+L   S L   P   G+   LR                       L+L
Sbjct: 749 PSFGDAINLQKLLLRYCSNLVELPSSIGNAINLRELDLYYCSSLIRLPSSIGNAINLLIL 808

Query: 671 DKTGIST---LHSSIKHLVGLICLNLTNCTMLSSLPNEVGNLRSLDTLLLGGCVNLDKIP 720
           D  G S    L SSI + + L  L+L  C  L  LP+ +GN  +L  LLL  C +L ++P
Sbjct: 809 DLNGCSNLLELPSSIGNAINLQKLDLRRCAKLLELPSSIGNAINLQNLLLDDCSSLLELP 868