HG10004270 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10004270 vs. NCBI nr
Match: XP_038884508.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Benincasa hispida])

HSP 1 Score: 1362.1 bits (3524), Expect = 0.0e+00
Identity = 695/865 (80.35%), Postives = 718/865 (83.01%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIELGSRMSHHRQMNPFMLPFST 60
           MLPSPCVLPS ALASLSLSAKK FT DSRNQWPSLRQ++  GS+MS+HRQMNPFMLPFST
Sbjct: 1   MLPSPCVLPSPALASLSLSAKKIFTLDSRNQWPSLRQELNFGSKMSNHRQMNPFMLPFST 60

Query: 61  YKRVHSPAISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLNLL 120
           YK+VHSPAISSV+TDDT  VSS DES ENIGILSHD  LE FKDHFKYRVGRYADLLNLL
Sbjct: 61  YKKVHSPAISSVVTDDTPKVSSPDESTENIGILSHDPGLESFKDHFKYRVGRYADLLNLL 120

Query: 121 DRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNEFG 180
           D+HEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGW+G+NHRMEKNEFG
Sbjct: 121 DKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWDGTNHRMEKNEFG 180

Query: 181 IWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP------- 240
           IWSIKIRDL  KPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPT+F AP       
Sbjct: 181 IWSIKIRDLSGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVYWD 240

Query: 241 ------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNTVQL 300
                 YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD +  RIKENNYNTVQL
Sbjct: 241 PPPLERYEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQL 300

Query: 301 MAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNNVT 360
           MAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLK L  KAHGLGLRVLMDVVHSHASNNVT
Sbjct: 301 MAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNNVT 360

Query: 361 DGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGF 420
           DGLNGFDVGQSTQDSYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGF
Sbjct: 361 DGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGF 420

Query: 421 RFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAEDV 480
           RFDGVTSMLYHHHGINMAFTG     FSEATDVDAVVYLMLANNLTHSILPDATVIAEDV
Sbjct: 421 RFDGVTSMLYHHHGINMAFTGTYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAEDV 480

Query: 481 SGMPGLGR------------------------------------------VSRGYSEKCI 540
           SGMPGLGR                                           +R YSEKCI
Sbjct: 481 SGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEKCI 540

Query: 541 SYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGGEG 600
           SYAESHDQSIVGDKTIAFLLMDKEMYSGMSC TDASPVVERGI+LHKMIHF+TMALGGEG
Sbjct: 541 SYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTDASPVVERGISLHKMIHFITMALGGEG 600

Query: 601 YLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY-- 660
           YLNFMGNE                G P  +  PREGNGWSYDKCRRQWNLPDTDHLRY  
Sbjct: 601 YLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKF 660

Query: 661 --------------------------------KVIVFERGDLVFVFNFHPINTYDGYKVG 720
                                           KVIVFERGDLVFVFNFHPINTYDGYKVG
Sbjct: 661 LNAFDRAMNSLDDKFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPINTYDGYKVG 720

Query: 721 CDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSPART 773
           CDLPGKYRVALDSDAWDFGGHGRVGH +DHFTSPEGIPGV ETNFNNRPNSFKVLSPART
Sbjct: 721 CDLPGKYRVALDSDAWDFGGHGRVGHDVDHFTSPEGIPGVSETNFNNRPNSFKVLSPART 780

BLAST of HG10004270 vs. NCBI nr
Match: XP_008464849.1 (PREDICTED: 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like isoform X1 [Cucumis melo] >ADN33992.1 starch branching enzyme [Cucumis melo subsp. melo])

HSP 1 Score: 1329.3 bits (3439), Expect = 0.0e+00
Identity = 685/874 (78.38%), Postives = 712/874 (81.46%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIELG--SRMSHHRQMNPFMLPF 60
           ML SPCVLPSTA+ASLSLSAKK F SDSRNQWPSLRQQ EL   SRMSHHRQMNPF+ P+
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLN 120
           STYKRVHSPAISSVMTDDTSTVSS DESMENIGILSHD  L+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNE 180
           LLD+HEGGLDEFA+GYLKFGFNREEDGIVYREWAPAAQEAQI+GDFNGWNG+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 FGIWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP----- 240
           FGIWSIKI DLG KPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPT+F AP     
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 --------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNTV 300
                   YEFKHPRPAKP  PRVYEAHVGMSSSEPRVSSYREFAD +  RIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLK L  KAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQSTQDSYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGI+MAFTGN    FSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGR------------------------------------------VSRGYSEK 540
           DVSGMPGLGR                                           +R YSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSC  +ASPVVERGIALHKMIHF+TMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY 660
           EGYLNFMGNE                G P  +  PREGNGWSYDKCRRQWNL DTDHLRY
Sbjct: 601 EGYLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRY 660

Query: 661 ----------------------------------KVIVFERGDLVFVFNFHPINTYDGYK 720
                                             KVIVFERGDLVFVFNFHPINTYDGYK
Sbjct: 661 KFLNAFDRAMNALDEKFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYK 720

Query: 721 VGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 780
           VGCDLPGKYRVALDSDA DFGG+GRVGH IDHFTSPEGIPGVPETNFNNRPNSFKVLSPA
Sbjct: 721 VGCDLPGKYRVALDSDAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 780

BLAST of HG10004270 vs. NCBI nr
Match: XP_004144236.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic [Cucumis sativus] >KGN47537.1 hypothetical protein Csa_019021 [Cucumis sativus])

HSP 1 Score: 1322.8 bits (3422), Expect = 0.0e+00
Identity = 681/867 (78.55%), Postives = 708/867 (81.66%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIELG--SRMSHHRQMNPFMLPF 60
           ML S CVLPSTA+ASLSLSAKK   SDSRNQWPSLRQQ EL   SRMSHHRQMNPF+ P+
Sbjct: 1   MLLSSCVLPSTAVASLSLSAKKIIISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFVFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLN 120
           STYKRVHSPAISSVMT+DTSTVSS DESMENIGILSHD  L+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTEDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNE 180
           LLD+HEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQI+GDFNGW+G+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180

Query: 181 FGIWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP----- 240
           FGIWSIK+ DLG KPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPT+F AP     
Sbjct: 181 FGIWSIKVYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 --------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNTV 300
                   YEFKHPRPAKP APRVYEAHVGMSSSEPRV+SYREFAD +  RIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLK L  KAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQS+QDSYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGINM F+GN    FSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFSGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGR------------------------------------------VSRGYSEK 540
           DVSGMPGLGR                                           +R YSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSC  +ASPVVERGIALHKMIHF+TMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY 660
           EGYLNFMGNE                G P  +  PREGNGWSYDKCRRQWNLPDTDHLRY
Sbjct: 601 EGYLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRY 660

Query: 661 ----------------------------------KVIVFERGDLVFVFNFHPINTYDGYK 720
                                             KVIVFERGDLVFVFNFHP+NTYDGYK
Sbjct: 661 KFLNAFDSAMNALDEKFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYK 720

Query: 721 VGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 772
           VGCDLPGKYRVALDSDA DFGGHGRVGH IDHFTSPEGIPGVPETNFNNRPNSFK+LSPA
Sbjct: 721 VGCDLPGKYRVALDSDASDFGGHGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKILSPA 780

BLAST of HG10004270 vs. NCBI nr
Match: XP_022153149.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Momordica charantia])

HSP 1 Score: 1265.0 bits (3272), Expect = 0.0e+00
Identity = 647/873 (74.11%), Postives = 695/873 (79.61%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIEL--GSRMSHHRQMNPFMLPF 60
           ML S CVLP T+LASLSLSAKK F++ SRN+WPSLR+QIEL   SRMSHHR MNPFM P 
Sbjct: 1   MLASSCVLPPTSLASLSLSAKKNFSAGSRNKWPSLRRQIELIFVSRMSHHRHMNPFMHPI 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLN 120
           STYKR HS AIS VMTD+  T++SADE MEN+GILSHD  LEPFKDHFKYR+GRYA+LL+
Sbjct: 61  STYKRGHSSAISCVMTDNAPTITSADEIMENMGILSHDPGLEPFKDHFKYRIGRYANLLS 120

Query: 121 LLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNE 180
           LL++HEGGLDEFA+GYLKFGFNREE GIVYREWAPAAQEAQIIGDFNGW+GSNH+MEKNE
Sbjct: 121 LLEKHEGGLDEFAQGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180

Query: 181 FGIWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP----- 240
           FGIWSIK+ D G KPAI+HNSRVKFRFKHGNGVWIDRIPAWI+YATVDPTRF AP     
Sbjct: 181 FGIWSIKLPDNGGKPAITHNSRVKFRFKHGNGVWIDRIPAWIRYATVDPTRFAAPYDGVY 240

Query: 241 --------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNTV 300
                   YEF+HPRPAKP+APR+YEAHVGMSSSEPRV+SYREFAD +  RIKENNYNTV
Sbjct: 241 WDPPPLERYEFEHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFILPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLK L  KAHGLGLRVLMDV+HSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVIHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQS+Q SYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEY+FD
Sbjct: 361 VTDGLNGFDVGQSSQGSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYKFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGINM FTGN    FSEATDVDAVVYLMLANNL HSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPDATVIAE 480

Query: 481 DVSGMPGLGR------------------------------------------VSRGYSEK 540
           DVSGMPGLGR                                           +R YSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGG 600
           CISYAESHDQ+IVGDKT+AFLLMDKEMYSGMSC T+ASP+VERGIALHKMIHF+TMALGG
Sbjct: 541 CISYAESHDQAIVGDKTVAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY 660
           EGYLNFMGNE                G P  +  PREGNGWSYDKCRRQWNLPDTDHLRY
Sbjct: 601 EGYLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRY 660

Query: 661 ----------------------------------KVIVFERGDLVFVFNFHPINTYDGYK 720
                                             KVIVFERGDLVFVFNFHP+NTYDGYK
Sbjct: 661 KFLNAFDRAMNLLDDKFSFLASSKQILSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYK 720

Query: 721 VGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 777
           VGCDLPGKYRVALDSDAWDFGGHGR+GH  DHFTSPEGIPGVPETNFNNRPNSFKVLSPA
Sbjct: 721 VGCDLPGKYRVALDSDAWDFGGHGRIGHDADHFTSPEGIPGVPETNFNNRPNSFKVLSPA 780

BLAST of HG10004270 vs. NCBI nr
Match: XP_023546757.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1238.0 bits (3202), Expect = 0.0e+00
Identity = 645/869 (74.22%), Postives = 678/869 (78.02%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQ--IELGSRMSHHRQMNPFMLPF 60
           ML SPCVLPSTALASLSLSAKK FTSDSRNQ  SLRQQ  +  GSRMSHHRQM+PFM PF
Sbjct: 1   MLASPCVLPSTALASLSLSAKKNFTSDSRNQCSSLRQQFVLNFGSRMSHHRQMHPFMAPF 60

Query: 61  STYKRVHSPA-ISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLL 120
           STYKRVHSPA ISSVMTDDTSTVSSADE+M++IGIL HD  LEPFKDHFKYR+GRY+D+L
Sbjct: 61  STYKRVHSPAIISSVMTDDTSTVSSADENMKDIGILRHDPSLEPFKDHFKYRIGRYSDVL 120

Query: 121 NLLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKN 180
           NL D+HEGGLDEFARGYLKFGFNRE DGIVYREWAP AQEAQIIGDFNGW+GSNHRMEKN
Sbjct: 121 NLFDKHEGGLDEFARGYLKFGFNREGDGIVYREWAPVAQEAQIIGDFNGWDGSNHRMEKN 180

Query: 181 EFGIWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP---- 240
           EFGIWSI I D+  KP+I+HNSRVKFRFKHGNGVWIDRIPAWIKYAT DPTRFGAP    
Sbjct: 181 EFGIWSINIPDIDGKPSIAHNSRVKFRFKHGNGVWIDRIPAWIKYATADPTRFGAPYDGV 240

Query: 241 ---------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNT 300
                    YEFKHPRPAKP+APR+YEAHVGMSSSEPRV+SYREFAD +  RIKENNYNT
Sbjct: 241 YWEPPPLERYEFKHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNT 300

Query: 301 VQLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASN 360
           VQLMAIMEHSYYASFGYH+TNFFA+SSRSGTPEDLK L  KAHGLGLRVLMD+VHSHASN
Sbjct: 301 VQLMAIMEHSYYASFGYHVTNFFAISSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASN 360

Query: 361 NVTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQF 420
           NVTDGLNGFDVGQSTQDSYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEYQF
Sbjct: 361 NVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQF 420

Query: 421 DGFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIA 480
           DGFRFDGVTSMLYHHHGIN AFTGN    FSEATDVDAVVYLMLANNL HSILP+ATVIA
Sbjct: 421 DGFRFDGVTSMLYHHHGINTAFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPNATVIA 480

Query: 481 EDVSGMPGLG------------------------------------------RVSRGYSE 540
           EDVSGMPGLG                                            +R YSE
Sbjct: 481 EDVSGMPGLGIPVSEGGIGFDYRLQMAIPDKWIDYVKNKNDEEWSMGEISWNLTNRRYSE 540

Query: 541 KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALG 600
           KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSC T+ASPVVERGIALHKMIHF+T+ALG
Sbjct: 541 KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTEASPVVERGIALHKMIHFITLALG 600

Query: 601 GEGYLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLR 660
           GEGYLNFMGNE                G P  +  PREGNGWSYDKCRRQWNLPDTDHLR
Sbjct: 601 GEGYLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLR 660

Query: 661 Y----------------------------------KVIVFERGDLVFVFNFHPINTYDGY 720
           Y                                  KVIVFERGDLVFVFNFHPINTYDGY
Sbjct: 661 YKFLNAFDRAMNSLDDKFSFLASSKQIVSCTSEEEKVIVFERGDLVFVFNFHPINTYDGY 720

Query: 721 KVGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSP 774
           KVGCD+PGKYRVALDSDA +FGG GRVGH IDHFTSPEGIPGVP+TNFNNRPNSFKVLSP
Sbjct: 721 KVGCDIPGKYRVALDSDASEFGGRGRVGHDIDHFTSPEGIPGVPKTNFNNRPNSFKVLSP 780

BLAST of HG10004270 vs. ExPASy Swiss-Prot
Match: Q41059 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic (Fragment) OS=Pisum sativum OX=3888 GN=SBEII PE=1 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 6.6e-286
Identity = 526/834 (63.07%), Postives = 592/834 (70.98%), Query Frame = 0

Query: 25  TSDSRNQWPSLRQ---QIELGSRMSHHRQMNPFMLPFSTYKRVHSPAIS-SVMTDDTSTV 84
           T +S+N+    +Q   ++ LG +  +  ++  F    S Y++V S     SVMTDD ST+
Sbjct: 7   THNSKNKQYLAKQKPVELTLGYQNPNGCKVCSFGSKGSIYQKVSSGFKGVSVMTDDKSTM 66

Query: 85  SSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLNLLDRHEGGLDEFARGYLKFGFN 144
            S +E  ENIGIL+ DS LEPFKDHFKYR+ RY     L++ +EGGL EFA+GYLKFGFN
Sbjct: 67  PSVEEDFENIGILNVDSSLEPFKDHFKYRLKRYLHQKKLIEEYEGGLQEFAKGYLKFGFN 126

Query: 145 REEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNEFGIWSIKIRDLGEKPAISHNSR 204
           REEDGI YREWAPAAQEAQIIGDFNGWNGSN  MEK++FG+WSI+I D    PAI HNSR
Sbjct: 127 REEDGISYREWAPAAQEAQIIGDFNGWNGSNLHMEKDQFGVWSIQIPDADGNPAIPHNSR 186

Query: 205 VKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP-------------YEFKHPRPAKPKAP 264
           VKFRFKH +GVW+DRIPAWIKYATVDPTRF AP             Y+FKHPRP KPKAP
Sbjct: 187 VKFRFKHSDGVWVDRIPAWIKYATVDPTRFAAPYDGVYWDPPLSERYQFKHPRPPKPKAP 246

Query: 265 RVYEAHVGMSSSEPRVSSYREFA-DLFTRIKENNYNTVQLMAIMEHSYYASFGYHITN-F 324
           R+YEAHVGMSSSEPR++SYREFA D+  RI+ENNYNTVQLMA+MEHSYYASF YH+T  F
Sbjct: 247 RIYEAHVGMSSSEPRINSYREFADDVLPRIRENNYNTVQLMAVMEHSYYASFWYHVTKPF 306

Query: 325 FAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNNVTDGLNGFDVGQSTQDSYFHT 384
           FAVSSRSG+PEDLK L  KAH LGL VLMDV+HSHASNNVTDGLNGFDVGQS+Q SYFH 
Sbjct: 307 FAVSSRSGSPEDLKYLIDKAHSLGLNVLMDVIHSHASNNVTDGLNGFDVGQSSQQSYFHA 366

Query: 385 GDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAF 444
           GDRGYHK WDSRLFNYANW+   FLLSN+RWWLEEY+FDGFRFDGVTSMLYHHHGINMAF
Sbjct: 367 GDRGYHKLWDSRLFNYANWK-SSFLLSNLRWWLEEYKFDGFRFDGVTSMLYHHHGINMAF 426

Query: 445 TGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGMPGLGR----------- 504
           TG+    FSE TDVDAVVYLMLAN+L H ILPDAT IAEDVSGMPGLGR           
Sbjct: 427 TGDYNEYFSEETDVDAVVYLMLANSLVHDILPDATDIAEDVSGMPGLGRPVSEVGIGFDY 486

Query: 505 -------------------------------VSRGYSEKCISYAESHDQSIVGDKTIAFL 564
                                           +R Y+EKC+SYAESHDQSIVGDKTIAFL
Sbjct: 487 RLAMAIPDKWIDYLKNKKDSEWSMKEISLNLTNRRYTEKCVSYAESHDQSIVGDKTIAFL 546

Query: 565 LMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGGEGYLNFMGNEVNLVSNSADQS 624
           LMD+EMYS MSC T  SP +ERGI+LHKMIHF+T+ALGGEGYLNFMGNE           
Sbjct: 547 LMDEEMYSSMSCLTMLSPTIERGISLHKMIHFITLALGGEGYLNFMGNE----------- 606

Query: 625 LGLSLGIPSGL-IPREGNGWSYDKCR-RQWNLPDTDHLRY-------------------- 684
                G P  +  PREGNGWSY+KCR  QWNL DT+HLRY                    
Sbjct: 607 ----FGHPEWIDFPREGNGWSYEKCRLTQWNLVDTNHLRYKFMNAFDRAMNLLDDKFSIL 666

Query: 685 --------------KVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDSDAWDF 744
                         KVIVFERGDLVFVFNFHP NTY+GYKVGCDLPGKYRVALDSDA +F
Sbjct: 667 ASTKQIVSSTNNEDKVIVFERGDLVFVFNFHPENTYEGYKVGCDLPGKYRVALDSDATEF 726

Query: 745 GGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYRVDESKEKENDDL 751
           GGHGRVGH  D FTSPEGIPG+PETNFNNRPNSFKVLSP  TCVVYYRVDE +E+ N+  
Sbjct: 727 GGHGRVGHDADQFTSPEGIPGIPETNFNNRPNSFKVLSPPHTCVVYYRVDERQEESNNPN 786

BLAST of HG10004270 vs. ExPASy Swiss-Prot
Match: P30924 (1,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2)

HSP 1 Score: 959.9 bits (2480), Expect = 1.7e-278
Identity = 478/706 (67.71%), Postives = 532/706 (75.35%), Query Frame = 0

Query: 65  HSPAISSVMTDDTSTVSSADESM--ENIGILSHDSDLEPFKDHFKYRVGRYADLLNLLDR 124
           HS AIS+V+TDD ST++  +E +  ENIG+L+ D  LEP+ DHF++R+ RY D   L+++
Sbjct: 69  HSSAISAVLTDDNSTMAPLEEDVKTENIGLLNLDPTLEPYLDHFRHRMKRYVDQKMLIEK 128

Query: 125 HEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNEFGIW 184
           +EG L+EFA+GYLKFGFNRE+  IVYREWAPAAQE ++IGDFNGWNGSNH MEK++FG+W
Sbjct: 129 YEGPLEEFAQGYLKFGFNREDGCIVYREWAPAAQEDEVIGDFNGWNGSNHMMEKDQFGVW 188

Query: 185 SIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAPYE------- 244
           SI+I D+  KP I HNSRVKFRFKHGNGVW+DRIPAWIKYAT D T+F APY+       
Sbjct: 189 SIRIPDVDSKPVIPHNSRVKFRFKHGNGVWVDRIPAWIKYATADATKFAAPYDGVYWDPP 248

Query: 245 ------FKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFA-DLFTRIKENNYNTVQLMA 304
                 FK+PRP KP+APR+YEAHVGMSSSEPRV+SYREFA D+  RIK NNYNTVQLMA
Sbjct: 249 PSERYHFKYPRPPKPRAPRIYEAHVGMSSSEPRVNSYREFADDVLPRIKANNYNTVQLMA 308

Query: 305 IMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNNVTDG 364
           IMEHSYY SFGYH+TNFFAVSSR G PEDLK L  KAH LGL+VL+DVVHSHASNNVTDG
Sbjct: 309 IMEHSYYGSFGYHVTNFFAVSSRYGNPEDLKYLIDKAHSLGLQVLVDVVHSHASNNVTDG 368

Query: 365 LNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRF 424
           LNGFD+GQ +Q+SYFH G+RGYHK WDSRLFNYANWEVLRFLLSN+RWWLEEY FDGFRF
Sbjct: 369 LNGFDIGQGSQESYFHAGERGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYNFDGFRF 428

Query: 425 DGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAEDVSG 484
           DG+TSMLY HHGINM FTGN    FSEATDVDAVVYLMLANNL H I PDATVIAEDVSG
Sbjct: 429 DGITSMLYVHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHKIFPDATVIAEDVSG 488

Query: 485 MPGLGR------------------------------------------VSRGYSEKCISY 544
           MPGLGR                                           +R Y+EKCI+Y
Sbjct: 489 MPGLGRPVSEGGIGFDYRLAMAIPDKWIDYLKNKNDEDWSMKEVTSSLTNRRYTEKCIAY 548

Query: 545 AESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGGEGYL 604
           AESHDQSIVGDKTIAFLLMDKEMYSGMSC TDASPVV+RGIALHKMIHF TMALGGEGYL
Sbjct: 549 AESHDQSIVGDKTIAFLLMDKEMYSGMSCLTDASPVVDRGIALHKMIHFFTMALGGEGYL 608

Query: 605 NFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY---- 664
           NFMGNE                G P  +  PREGN WSYDKCRRQWNL D++HLRY    
Sbjct: 609 NFMGNE---------------FGHPEWIDFPREGNNWSYDKCRRQWNLADSEHLRYKFMN 668

Query: 665 ------------------------------KVIVFERGDLVFVFNFHPINTYDGYKVGCD 676
                                         KV+VFERGDLVFVFNFHP NTY+GYKVGCD
Sbjct: 669 AFDRAMNSLDEKFSFLASGKQIVSSMDDDNKVVVFERGDLVFVFNFHPKNTYEGYKVGCD 728

BLAST of HG10004270 vs. ExPASy Swiss-Prot
Match: Q01401 (1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SBE1 PE=1 SV=2)

HSP 1 Score: 927.2 bits (2395), Expect = 1.3e-268
Identity = 474/793 (59.77%), Postives = 543/793 (68.47%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIELGSRMSHHRQMNPFMLPFST 60
           +LPS    PS  +A    + +    S  R  WP               +    F +P + 
Sbjct: 15  LLPSLADRPSPGIAGGGGNVRLSVVSSPRRSWPG--------------KVKTNFSVPATA 74

Query: 61  YKRVHSPAISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLNLL 120
            K                T+ +  E ++++ I   D  LE FKDHF YR+ RY D   L+
Sbjct: 75  RK--------------NKTMVTVVEEVDHLPIYDLDPKLEEFKDHFNYRIKRYLDQKCLI 134

Query: 121 DRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNEFG 180
           ++HEGGL+EF++GYLKFG N  +   +YREWAPAAQEAQ+IG+FN WNG+ H+MEK++FG
Sbjct: 135 EKHEGGLEEFSKGYLKFGINTVDGATIYREWAPAAQEAQLIGEFNNWNGAKHKMEKDKFG 194

Query: 181 IWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAPYE----- 240
           IWSIKI  +  KPAI HNS+VKFRF+HG G W+DRIPAWI+YAT D ++FGAPY+     
Sbjct: 195 IWSIKISHVNGKPAIPHNSKVKFRFRHGGGAWVDRIPAWIRYATFDASKFGAPYDGVHWD 254

Query: 241 --------FKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNTVQL 300
                   FKHPRP KP APR+YEAHVGMS  EP VS+YREFAD +  RI+ NNYNTVQL
Sbjct: 255 PPACERYVFKHPRPPKPDAPRIYEAHVGMSGEEPEVSTYREFADNVLPRIRANNYNTVQL 314

Query: 301 MAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNNVT 360
           MAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLK L  KAH LGLRVLMDVVHSHASNNVT
Sbjct: 315 MAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLVDKAHSLGLRVLMDVVHSHASNNVT 374

Query: 361 DGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGF 420
           DGLNG+DVGQ+T +SYFHTGDRGYHK WDSRLFNYANWEVLRFLLSN+R+W++E+ FDGF
Sbjct: 375 DGLNGYDVGQNTHESYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNLRYWMDEFMFDGF 434

Query: 421 RFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAEDV 480
           RFDGVTSMLYHHHGIN  FTGN    FS  TDVDA+VY+MLAN+L H +LP+AT++AEDV
Sbjct: 435 RFDGVTSMLYHHHGINKGFTGNYKEYFSLDTDVDAIVYMMLANHLMHKLLPEATIVAEDV 494

Query: 481 SGMPGLGR------------------------------------------VSRGYSEKCI 540
           SGMP L R                                           +R Y+EKCI
Sbjct: 495 SGMPVLCRPVDEGGVGFDFRLAMAIPDRWIDYLKNKEDRKWSMSEIVQTLTNRRYTEKCI 554

Query: 541 SYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGGEG 600
           +YAESHDQSIVGDKTIAFLLMDKEMY+GMS    ASP + RGIAL KMIHF+TMALGG+G
Sbjct: 555 AYAESHDQSIVGDKTIAFLLMDKEMYTGMSDLQPASPTINRGIALQKMIHFITMALGGDG 614

Query: 601 YLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY-- 660
           YLNFMGNE                G P  +  PREGN WSYDKCRRQW+L DTDHLRY  
Sbjct: 615 YLNFMGNE---------------FGHPEWIDFPREGNNWSYDKCRRQWSLVDTDHLRYKY 674

Query: 661 --------------------------------KVIVFERGDLVFVFNFHPINTYDGYKVG 701
                                           KVIVFERGDLVFVFNFHP  TY GYKVG
Sbjct: 675 MNAFDQAMNALEEEFSFLSSSKQIVSDMNEKDKVIVFERGDLVFVFNFHPNKTYKGYKVG 734

BLAST of HG10004270 vs. ExPASy Swiss-Prot
Match: Q08047 (1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic OS=Zea mays OX=4577 GN=SBE1 PE=1 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 1.5e-181
Identity = 347/698 (49.71%), Postives = 430/698 (61.60%), Query Frame = 0

Query: 92  ILSHDSDLEPFKDHFKYRVGRYADLLNLLDRHEGGLDEFARGYLKFGFNREEDGIVYREW 151
           I   D  L+ +K H +YR   Y  + + +D HEGGL+ F+R Y KFGFN   +GI YREW
Sbjct: 121 IFQIDPMLQGYKYHLEYRYSLYRRIRSDIDEHEGGLEAFSRSYEKFGFNASAEGITYREW 180

Query: 152 APAAQEAQIIGDFNGWNGSNHRMEKNEFGIWSIKIRDLGEKPA-ISHNSRVKFRFKHGNG 211
           AP A  A ++GD N W+ +  RM KNEFG+W I + +  +  + I H SRVK R    +G
Sbjct: 181 APGAFSAALVGDVNNWDPNADRMSKNEFGVWEIFLPNNADGTSPIPHGSRVKVRMDTPSG 240

Query: 212 VWIDRIPAWIKYATVDPTRF-----------GAPYEFKHPRPAKPKAPRVYEAHVGMSSS 271
           +  D IPAWIKY+   P                 Y F+H +P +PK+ R+YE HVGMSS 
Sbjct: 241 I-KDSIPAWIKYSVQAPGEIPYDGIYYDPPEEVKYVFRHAQPKRPKSLRIYETHVGMSSP 300

Query: 272 EPRVSSYREFAD-LFTRIKENNYNTVQLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDL 331
           EP++++Y  F D +  RIK+  YN VQ+MAI EHSYY SFGYH+TNFFA SSR GTPEDL
Sbjct: 301 EPKINTYVNFRDEVLPRIKKLGYNAVQIMAIQEHSYYGSFGYHVTNFFAPSSRFGTPEDL 360

Query: 332 K-ILCKAHGLGLRVLMDVVHSHASNNVTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRL 391
           K ++ +AH LGL VLMDVVHSHAS+N  DGLNGFD    T   YFH+G RG+H  WDSRL
Sbjct: 361 KSLIDRAHELGLLVLMDVVHSHASSNTLDGLNGFD---GTDTHYFHSGPRGHHWMWDSRL 420

Query: 392 FNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATD 451
           FNY NWEVLRFLLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+ + FTGN    F  ATD
Sbjct: 421 FNYGNWEVLRFLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVTFTGNFNEYFGFATD 480

Query: 452 VDAVVYLMLANNLTHSILPDATVIAEDVSGMP---------GLG---------------- 511
           VDAVVYLML N+L H + P+A  I EDVSGMP         G+G                
Sbjct: 481 VDAVVYLMLVNDLIHGLYPEAVTIGEDVSGMPTFALPVHDGGVGFDYRMHMAVADKWIDL 540

Query: 512 ----------------RVSRGYSEKCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFT 571
                             +R + EKC++YAESHDQ++VGDKTIAF LMDK+MY  M+   
Sbjct: 541 LKQSDETWKMGDIVHTLTNRRWLEKCVTYAESHDQALVGDKTIAFWLMDKDMYDFMALDR 600

Query: 572 DASPVVERGIALHKMIHFLTMALGGEGYLNFMGNEVNLVSNSADQSLGLSLG---IPSG- 631
            ++P ++RGIALHKMI  +TM LGGEGYLNFMGNE         + +    G   +PSG 
Sbjct: 601 PSTPTIDRGIALHKMIRLITMGLGGEGYLNFMGNEF-----GHPEWIDFPRGPQRLPSGK 660

Query: 632 LIPREGNGWSYDKCRRQWNLPDTDHLRY-------------------------------- 691
            IP  GN  SYDKCRR+++L D D+LRY                                
Sbjct: 661 FIP--GNNNSYDKCRRRFDLGDADYLRYHGMQEFDQAMQHLEQKYEFMTSDHQYISRKHE 720

Query: 692 --KVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDSDAWDFGGHGRVGHSIDH 696
             KVIVFE+GDLVFVFNFH  N+Y  Y++GC  PG Y+V LDSDA  FGG  R+ H+ +H
Sbjct: 721 EDKVIVFEKGDLVFVFNFHCNNSYFDYRIGCRKPGVYKVVLDSDAGLFGGFSRIHHAAEH 780

BLAST of HG10004270 vs. ExPASy Swiss-Prot
Match: O23647 (1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SBE2.1 PE=1 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 2.0e-181
Identity = 346/705 (49.08%), Postives = 432/705 (61.28%), Query Frame = 0

Query: 96  DSDLEPFKDHFKYRVGRYADLLNLLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAA 155
           D  L   ++H  YR G+Y  L   +D++EGGL+ F+RGY  FGF R   GI YREWAP A
Sbjct: 164 DPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTRSATGITYREWAPGA 223

Query: 156 QEAQIIGDFNGWNGSNHRMEKNEFGIWSIKIRDLGE-KPAISHNSRVKFRFKHGNGVWID 215
           + A +IGDFN WN  +  M +N+FG+W I + +  +  PAI H SRVK R    +G+  D
Sbjct: 224 KAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSGI-KD 283

Query: 216 RIPAWIKYATVDP-------TRFGAP----YEFKHPRPAKPKAPRVYEAHVGMSSSEPRV 275
            IPAWIKY+   P         +  P    Y FKHPRP KP + R+YE+HVGMSS+EP++
Sbjct: 284 SIPAWIKYSVQPPGEIPYNGVYYDPPEEDKYAFKHPRPKKPTSLRIYESHVGMSSTEPKI 343

Query: 276 SSYREFA-DLFTRIKENNYNTVQLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLK-IL 335
           ++Y  F  D+  RIK+  YN VQ+MAI EH+YYASFGYH+TNFFA SSR GTP+DLK ++
Sbjct: 344 NTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKSLI 403

Query: 336 CKAHGLGLRVLMDVVHSHASNNVTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYA 395
            KAH LGL VLMD+VHSHAS N  DGL+ FD    T   YFH+G RGYH  WDSRLFNY 
Sbjct: 404 DKAHELGLVVLMDIVHSHASKNTLDGLDMFD---GTDGQYFHSGSRGYHWMWDSRLFNYG 463

Query: 396 NWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAV 455
           +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+ + FTGN    F  +TDVDAV
Sbjct: 464 SWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVDAV 523

Query: 456 VYLMLANNLTHSILPDATVIAEDVSGMP---------GLG-------------------- 515
           VYLML N+L H + P+A V+ EDVSGMP         G+G                    
Sbjct: 524 VYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWIELLKKR 583

Query: 516 ------------RVSRGYSEKCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASP 575
                         +R + EKC+ YAESHDQ++VGDKTIAF LMDK+MY  M+    A+P
Sbjct: 584 DEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQATP 643

Query: 576 VVERGIALHKMIHFLTMALGGEGYLNFMGNEVN----LVSNSADQSLGLSLGIPSGLIPR 635
            V+RGIALHKMI  +TM LGGEGYLNFMGNE      +     DQ L      P G +  
Sbjct: 644 RVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRTDQHL------PDGRV-I 703

Query: 636 EGNGWSYDKCRRQWNLPDTDHLRY----------------------------------KV 695
            GN  SYDKCRR+++L D ++LRY                                  +V
Sbjct: 704 AGNNGSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRV 763

Query: 696 IVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSP 707
           IVFERG+L+FVFNFH  N+Y  Y++GC +PGKY++ LDSD   FGG  R+  S + FTS 
Sbjct: 764 IVFERGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFGGFNRLDDSAEFFTS- 823

BLAST of HG10004270 vs. ExPASy TrEMBL
Match: E5GBZ3 (1,4-alpha-glucan branching enzyme OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1329.3 bits (3439), Expect = 0.0e+00
Identity = 685/874 (78.38%), Postives = 712/874 (81.46%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIELG--SRMSHHRQMNPFMLPF 60
           ML SPCVLPSTA+ASLSLSAKK F SDSRNQWPSLRQQ EL   SRMSHHRQMNPF+ P+
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLN 120
           STYKRVHSPAISSVMTDDTSTVSS DESMENIGILSHD  L+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNE 180
           LLD+HEGGLDEFA+GYLKFGFNREEDGIVYREWAPAAQEAQI+GDFNGWNG+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 FGIWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP----- 240
           FGIWSIKI DLG KPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPT+F AP     
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 --------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNTV 300
                   YEFKHPRPAKP  PRVYEAHVGMSSSEPRVSSYREFAD +  RIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLK L  KAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQSTQDSYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGI+MAFTGN    FSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGR------------------------------------------VSRGYSEK 540
           DVSGMPGLGR                                           +R YSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSC  +ASPVVERGIALHKMIHF+TMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY 660
           EGYLNFMGNE                G P  +  PREGNGWSYDKCRRQWNL DTDHLRY
Sbjct: 601 EGYLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRY 660

Query: 661 ----------------------------------KVIVFERGDLVFVFNFHPINTYDGYK 720
                                             KVIVFERGDLVFVFNFHPINTYDGYK
Sbjct: 661 KFLNAFDRAMNALDEKFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYK 720

Query: 721 VGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 780
           VGCDLPGKYRVALDSDA DFGG+GRVGH IDHFTSPEGIPGVPETNFNNRPNSFKVLSPA
Sbjct: 721 VGCDLPGKYRVALDSDAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 780

BLAST of HG10004270 vs. ExPASy TrEMBL
Match: A0A1S3CMX9 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103502624 PE=3 SV=1)

HSP 1 Score: 1329.3 bits (3439), Expect = 0.0e+00
Identity = 685/874 (78.38%), Postives = 712/874 (81.46%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIELG--SRMSHHRQMNPFMLPF 60
           ML SPCVLPSTA+ASLSLSAKK F SDSRNQWPSLRQQ EL   SRMSHHRQMNPF+ P+
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLN 120
           STYKRVHSPAISSVMTDDTSTVSS DESMENIGILSHD  L+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNE 180
           LLD+HEGGLDEFA+GYLKFGFNREEDGIVYREWAPAAQEAQI+GDFNGWNG+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 FGIWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP----- 240
           FGIWSIKI DLG KPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPT+F AP     
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 --------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNTV 300
                   YEFKHPRPAKP  PRVYEAHVGMSSSEPRVSSYREFAD +  RIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLK L  KAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQSTQDSYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGI+MAFTGN    FSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGR------------------------------------------VSRGYSEK 540
           DVSGMPGLGR                                           +R YSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSC  +ASPVVERGIALHKMIHF+TMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY 660
           EGYLNFMGNE                G P  +  PREGNGWSYDKCRRQWNL DTDHLRY
Sbjct: 601 EGYLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRY 660

Query: 661 ----------------------------------KVIVFERGDLVFVFNFHPINTYDGYK 720
                                             KVIVFERGDLVFVFNFHPINTYDGYK
Sbjct: 661 KFLNAFDRAMNALDEKFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYK 720

Query: 721 VGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 780
           VGCDLPGKYRVALDSDA DFGG+GRVGH IDHFTSPEGIPGVPETNFNNRPNSFKVLSPA
Sbjct: 721 VGCDLPGKYRVALDSDAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 780

BLAST of HG10004270 vs. ExPASy TrEMBL
Match: A0A0A0KGQ9 (1,4-alpha-glucan branching enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G357030 PE=3 SV=1)

HSP 1 Score: 1322.8 bits (3422), Expect = 0.0e+00
Identity = 681/867 (78.55%), Postives = 708/867 (81.66%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIELG--SRMSHHRQMNPFMLPF 60
           ML S CVLPSTA+ASLSLSAKK   SDSRNQWPSLRQQ EL   SRMSHHRQMNPF+ P+
Sbjct: 1   MLLSSCVLPSTAVASLSLSAKKIIISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFVFPY 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLN 120
           STYKRVHSPAISSVMT+DTSTVSS DESMENIGILSHD  L+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTEDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNE 180
           LLD+HEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQI+GDFNGW+G+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180

Query: 181 FGIWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP----- 240
           FGIWSIK+ DLG KPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPT+F AP     
Sbjct: 181 FGIWSIKVYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 --------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNTV 300
                   YEFKHPRPAKP APRVYEAHVGMSSSEPRV+SYREFAD +  RIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLK L  KAHGLGLRVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQS+QDSYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGINM F+GN    FSEATDVDAVVYLMLANNLTHSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFSGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGR------------------------------------------VSRGYSEK 540
           DVSGMPGLGR                                           +R YSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGG 600
           CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSC  +ASPVVERGIALHKMIHF+TMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY 660
           EGYLNFMGNE                G P  +  PREGNGWSYDKCRRQWNLPDTDHLRY
Sbjct: 601 EGYLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRY 660

Query: 661 ----------------------------------KVIVFERGDLVFVFNFHPINTYDGYK 720
                                             KVIVFERGDLVFVFNFHP+NTYDGYK
Sbjct: 661 KFLNAFDSAMNALDEKFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYK 720

Query: 721 VGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 772
           VGCDLPGKYRVALDSDA DFGGHGRVGH IDHFTSPEGIPGVPETNFNNRPNSFK+LSPA
Sbjct: 721 VGCDLPGKYRVALDSDASDFGGHGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKILSPA 780

BLAST of HG10004270 vs. ExPASy TrEMBL
Match: A0A6J1DI71 (1,4-alpha-glucan branching enzyme OS=Momordica charantia OX=3673 GN=LOC111020717 PE=3 SV=1)

HSP 1 Score: 1265.0 bits (3272), Expect = 0.0e+00
Identity = 647/873 (74.11%), Postives = 695/873 (79.61%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIEL--GSRMSHHRQMNPFMLPF 60
           ML S CVLP T+LASLSLSAKK F++ SRN+WPSLR+QIEL   SRMSHHR MNPFM P 
Sbjct: 1   MLASSCVLPPTSLASLSLSAKKNFSAGSRNKWPSLRRQIELIFVSRMSHHRHMNPFMHPI 60

Query: 61  STYKRVHSPAISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLLN 120
           STYKR HS AIS VMTD+  T++SADE MEN+GILSHD  LEPFKDHFKYR+GRYA+LL+
Sbjct: 61  STYKRGHSSAISCVMTDNAPTITSADEIMENMGILSHDPGLEPFKDHFKYRIGRYANLLS 120

Query: 121 LLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKNE 180
           LL++HEGGLDEFA+GYLKFGFNREE GIVYREWAPAAQEAQIIGDFNGW+GSNH+MEKNE
Sbjct: 121 LLEKHEGGLDEFAQGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180

Query: 181 FGIWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP----- 240
           FGIWSIK+ D G KPAI+HNSRVKFRFKHGNGVWIDRIPAWI+YATVDPTRF AP     
Sbjct: 181 FGIWSIKLPDNGGKPAITHNSRVKFRFKHGNGVWIDRIPAWIRYATVDPTRFAAPYDGVY 240

Query: 241 --------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNTV 300
                   YEF+HPRPAKP+APR+YEAHVGMSSSEPRV+SYREFAD +  RIKENNYNTV
Sbjct: 241 WDPPPLERYEFEHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFILPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLK L  KAHGLGLRVLMDV+HSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVIHSHASNN 360

Query: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQS+Q SYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEY+FD
Sbjct: 361 VTDGLNGFDVGQSSQGSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYKFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIAE 480
           GFRFDGVTSMLYHHHGINM FTGN    FSEATDVDAVVYLMLANNL HSILPDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPDATVIAE 480

Query: 481 DVSGMPGLGR------------------------------------------VSRGYSEK 540
           DVSGMPGLGR                                           +R YSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALGG 600
           CISYAESHDQ+IVGDKT+AFLLMDKEMYSGMSC T+ASP+VERGIALHKMIHF+TMALGG
Sbjct: 541 CISYAESHDQAIVGDKTVAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600

Query: 601 EGYLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLRY 660
           EGYLNFMGNE                G P  +  PREGNGWSYDKCRRQWNLPDTDHLRY
Sbjct: 601 EGYLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRY 660

Query: 661 ----------------------------------KVIVFERGDLVFVFNFHPINTYDGYK 720
                                             KVIVFERGDLVFVFNFHP+NTYDGYK
Sbjct: 661 KFLNAFDRAMNLLDDKFSFLASSKQILSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYK 720

Query: 721 VGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSPA 777
           VGCDLPGKYRVALDSDAWDFGGHGR+GH  DHFTSPEGIPGVPETNFNNRPNSFKVLSPA
Sbjct: 721 VGCDLPGKYRVALDSDAWDFGGHGRIGHDADHFTSPEGIPGVPETNFNNRPNSFKVLSPA 780

BLAST of HG10004270 vs. ExPASy TrEMBL
Match: A0A6J1KG03 (1,4-alpha-glucan branching enzyme OS=Cucurbita maxima OX=3661 GN=LOC111493933 PE=3 SV=1)

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 646/870 (74.25%), Postives = 680/870 (78.16%), Query Frame = 0

Query: 1   MLPSPCVLPSTALASLSLSAKKFFTSDSRNQWPSLRQQIEL--GSRMSHHRQMNPFMLPF 60
           ML SPCVLPST LASLSLSAKK FTSDSRNQ  SLRQQI L  GSRMSHHRQM+PFM PF
Sbjct: 1   MLASPCVLPSTVLASLSLSAKKNFTSDSRNQCSSLRQQIVLNFGSRMSHHRQMHPFMDPF 60

Query: 61  STYKRVHSPA-ISSVMTDDTSTVSSADESMENIGILSHDSDLEPFKDHFKYRVGRYADLL 120
           STYKRV+ PA ISSVMTDDTST SSADE+M++IGIL HD  LEPFKDHFKYR+GRY+D+L
Sbjct: 61  STYKRVYRPAIISSVMTDDTSTASSADENMKDIGILRHDPSLEPFKDHFKYRIGRYSDVL 120

Query: 121 NLLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWNGSNHRMEKN 180
           NL D+HEGGLDEFARGYLKFGFNRE DGIVYREWAP AQEAQIIGDFNGW+GSNHRMEKN
Sbjct: 121 NLFDKHEGGLDEFARGYLKFGFNREGDGIVYREWAPVAQEAQIIGDFNGWDGSNHRMEKN 180

Query: 181 EFGIWSIKIRDLGEKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFGAP---- 240
           EFGIWSI I D+  KPAI+HNSRVKFRFKHGNG WIDRIPAWIKYATVDPTRFGAP    
Sbjct: 181 EFGIWSINIPDIDGKPAIAHNSRVKFRFKHGNGAWIDRIPAWIKYATVDPTRFGAPYDGV 240

Query: 241 ---------YEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFAD-LFTRIKENNYNT 300
                    YEFKHPRPAKPKAPR+YEAHVGMSSSEPRV+SYREFAD +  RIKENNYNT
Sbjct: 241 YWEPPPLERYEFKHPRPAKPKAPRIYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNT 300

Query: 301 VQLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKILC-KAHGLGLRVLMDVVHSHASN 360
           VQLMAIMEHSYYASFGYH+TNFFA+SSRSGTPEDLK L  KAHGLGLRVLMD+VHSHASN
Sbjct: 301 VQLMAIMEHSYYASFGYHVTNFFAMSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASN 360

Query: 361 NVTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQF 420
           NVTDGLNGFDVGQSTQDSYFHTGDRGYHK WDSRLFNYANWEVLRFLLSNIRWWLEEYQF
Sbjct: 361 NVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQF 420

Query: 421 DGFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAVVYLMLANNLTHSILPDATVIA 480
           DGFRFDGVTSMLYHHHGIN AFTGN    FSEATDVDAVVYLMLANNL HSILP+ATVIA
Sbjct: 421 DGFRFDGVTSMLYHHHGINTAFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPNATVIA 480

Query: 481 EDVSGMPGLG------------------------------------------RVSRGYSE 540
           EDVSGMPGLG                                            +R YSE
Sbjct: 481 EDVSGMPGLGIPVSEGGIGFDYRLQMAIPDKWIDYVKNKNDEEWSMGEISWNLTNRRYSE 540

Query: 541 KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASPVVERGIALHKMIHFLTMALG 600
           KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSC T+ASPVVERGIALHKMIHF+TMALG
Sbjct: 541 KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTEASPVVERGIALHKMIHFITMALG 600

Query: 601 GEGYLNFMGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWNLPDTDHLR 660
           GEGYLNFMGNE                G P  +  PREGNGWSYDKCRRQWNLPDTDHLR
Sbjct: 601 GEGYLNFMGNE---------------FGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLR 660

Query: 661 Y----------------------------------KVIVFERGDLVFVFNFHPINTYDGY 720
           Y                                  KVIVFERGDLVFVFNFHPINTYDGY
Sbjct: 661 YKFLNAFDRAMNSLDDKFSFLASSKQIVSCTSEEEKVIVFERGDLVFVFNFHPINTYDGY 720

Query: 721 KVGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSPEGIPGVPETNFNNRPNSFKVLSP 775
           KVGCD+PGKYRVALDSDA +FGG GRVGH IDHFTSPEGIPGVPETNFNNRPNSFKVLSP
Sbjct: 721 KVGCDIPGKYRVALDSDASEFGGRGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSP 780

BLAST of HG10004270 vs. TAIR 10
Match: AT2G36390.1 (starch branching enzyme 2.1 )

HSP 1 Score: 637.5 bits (1643), Expect = 1.4e-182
Identity = 346/705 (49.08%), Postives = 432/705 (61.28%), Query Frame = 0

Query: 96  DSDLEPFKDHFKYRVGRYADLLNLLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAA 155
           D  L   ++H  YR G+Y  L   +D++EGGL+ F+RGY  FGF R   GI YREWAP A
Sbjct: 164 DPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTRSATGITYREWAPGA 223

Query: 156 QEAQIIGDFNGWNGSNHRMEKNEFGIWSIKIRDLGE-KPAISHNSRVKFRFKHGNGVWID 215
           + A +IGDFN WN  +  M +N+FG+W I + +  +  PAI H SRVK R    +G+  D
Sbjct: 224 KAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSGI-KD 283

Query: 216 RIPAWIKYATVDP-------TRFGAP----YEFKHPRPAKPKAPRVYEAHVGMSSSEPRV 275
            IPAWIKY+   P         +  P    Y FKHPRP KP + R+YE+HVGMSS+EP++
Sbjct: 284 SIPAWIKYSVQPPGEIPYNGVYYDPPEEDKYAFKHPRPKKPTSLRIYESHVGMSSTEPKI 343

Query: 276 SSYREFA-DLFTRIKENNYNTVQLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLK-IL 335
           ++Y  F  D+  RIK+  YN VQ+MAI EH+YYASFGYH+TNFFA SSR GTP+DLK ++
Sbjct: 344 NTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKSLI 403

Query: 336 CKAHGLGLRVLMDVVHSHASNNVTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYA 395
            KAH LGL VLMD+VHSHAS N  DGL+ FD    T   YFH+G RGYH  WDSRLFNY 
Sbjct: 404 DKAHELGLVVLMDIVHSHASKNTLDGLDMFD---GTDGQYFHSGSRGYHWMWDSRLFNYG 463

Query: 396 NWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAV 455
           +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+ + FTGN    F  +TDVDAV
Sbjct: 464 SWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVDAV 523

Query: 456 VYLMLANNLTHSILPDATVIAEDVSGMP---------GLG-------------------- 515
           VYLML N+L H + P+A V+ EDVSGMP         G+G                    
Sbjct: 524 VYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWIELLKKR 583

Query: 516 ------------RVSRGYSEKCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASP 575
                         +R + EKC+ YAESHDQ++VGDKTIAF LMDK+MY  M+    A+P
Sbjct: 584 DEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQATP 643

Query: 576 VVERGIALHKMIHFLTMALGGEGYLNFMGNEVN----LVSNSADQSLGLSLGIPSGLIPR 635
            V+RGIALHKMI  +TM LGGEGYLNFMGNE      +     DQ L      P G +  
Sbjct: 644 RVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRTDQHL------PDGRV-I 703

Query: 636 EGNGWSYDKCRRQWNLPDTDHLRY----------------------------------KV 695
            GN  SYDKCRR+++L D ++LRY                                  +V
Sbjct: 704 AGNNGSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRV 763

Query: 696 IVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDSDAWDFGGHGRVGHSIDHFTSP 707
           IVFERG+L+FVFNFH  N+Y  Y++GC +PGKY++ LDSD   FGG  R+  S + FTS 
Sbjct: 764 IVFERGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFGGFNRLDDSAEFFTS- 823

BLAST of HG10004270 vs. TAIR 10
Match: AT5G03650.1 (starch branching enzyme 2.2 )

HSP 1 Score: 629.0 bits (1621), Expect = 5.0e-180
Identity = 341/686 (49.71%), Postives = 426/686 (62.10%), Query Frame = 0

Query: 96  DSDLEPFKDHFKYRVGRYADLLNLLDRHEGGLDEFARGYLKFGFNREEDGIVYREWAPAA 155
           D  L  + +H  YR G+Y  L   +D++EGGL+ F+RGY K GF+R + GI YREWAP A
Sbjct: 129 DPMLRTYNNHLDYRYGQYKRLREEIDKYEGGLEAFSRGYEKLGFSRSDAGITYREWAPGA 188

Query: 156 QEAQIIGDFNGWNGSNHRMEKNEFGIWSIKI-RDLGEKPAISHNSRVKFRFKHGNGVWID 215
           + A +IGDFN WN +   M +NEFG+W I +  +    PAI H SRVK R    +G+  D
Sbjct: 189 KAASLIGDFNNWNSNADIMTRNEFGVWEIFLPNNTDGSPAIPHGSRVKIRMDTPSGI-KD 248

Query: 216 RIPAWIKYATVDPTR-------FGAP----YEFKHPRPAKPKAPRVYEAHVGMSSSEPRV 275
            IPAWIK++   P         +  P    Y FKHP+P +PK+ R+YEAHVGMSS+EP V
Sbjct: 249 SIPAWIKFSVQAPGEIPFNGIYYDPPEEEKYVFKHPQPKRPKSLRIYEAHVGMSSTEPMV 308

Query: 276 SSYREFA-DLFTRIKENNYNTVQLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLK-IL 335
           ++Y  F  D+  RIK+  YN VQ+MAI EHSYYASFGYH+TNFFA SSR GTPE+LK ++
Sbjct: 309 NTYANFRDDVLPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEELKSLI 368

Query: 336 CKAHGLGLRVLMDVVHSHASNNVTDGLNGFDVGQSTQDSYFHTGDRGYHKRWDSRLFNYA 395
            +AH LGL VLMD+VHSHAS N  DGLN FD    T   YFH+G RGYH  WDSRLFNY 
Sbjct: 369 DRAHELGLVVLMDIVHSHASKNTLDGLNMFD---GTDAHYFHSGPRGYHWMWDSRLFNYG 428

Query: 396 NWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNIMR-FSEATDVDAV 455
           +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+++ FTGN    F   TDVDAV
Sbjct: 429 SWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLSVGFTGNYTEYFGLETDVDAV 488

Query: 456 VYLMLANNLTHSILPDATVIAEDVSGMP---------GLG-------------------- 515
            YLML N++ H + P+A  + EDVSGMP         G+G                    
Sbjct: 489 NYLMLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKR 548

Query: 516 ------------RVSRGYSEKCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCFTDASP 575
                         +R +SEKCISYAESHDQ++VGDKTIAF LMDK+MY  M+    ++P
Sbjct: 549 DEDWQMGDIIYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRPSTP 608

Query: 576 VVERGIALHKMIHFLTMALGGEGYLNFMGNEVNLVSNSADQSLGLSLGIPSGLIPREGNG 635
           +++RGIALHKMI  +TM LGGEGYLNFMGNE        D   G        +IP  GN 
Sbjct: 609 LIDRGIALHKMIRLITMGLGGEGYLNFMGNEFG-HPEWIDFPRGEQRLSDGSVIP--GNN 668

Query: 636 WSYDKCRRQWNLPDTDHLRY----------------------------------KVIVFE 692
           +SYDKCRR+++L D D+LRY                                  +VIVFE
Sbjct: 669 FSYDKCRRRFDLGDADYLRYRGLQEFDQAMQHLEENYGFMTSEHQFISRKDEADRVIVFE 728

BLAST of HG10004270 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 325.1 bits (832), Expect = 1.5e-88
Identity = 206/580 (35.52%), Postives = 305/580 (52.59%), Query Frame = 0

Query: 193 PAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFG-------------APYEFKHP 252
           PA+ H S+ +  F   +G  ++R+PAW  Y  V P   G             A Y++K+ 
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYS 393

Query: 253 RPAKPKAPRVYEAHVGMSSSEPRVSSYREFA-DLFTRIKENNYNTVQLMAIMEHSYYASF 312
           +P  P++ R+YE HVG+S SEP+VS++ EF   +   +K   YN +QL+ + EH  Y + 
Sbjct: 394 KPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTV 453

Query: 313 GYHITNFFAVSSRSGTPEDLK-ILCKAHGLGLRVLMDVVHSHASNNVTDGLNGFDVGQST 372
           GY +TNFFA SSR GTP+D K ++ +AHGLGL V +D+VHS+A+ +   GL+ FD    +
Sbjct: 454 GYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD---GS 513

Query: 373 QDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHH 432
            D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EYQ DG++F  + SM+Y H
Sbjct: 514 NDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTH 573

Query: 433 HGINMAFTGNIMRF-SEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGMPGLGR-VSR 492
           +G   +F  ++  + ++  D DA++YL+LAN + H   P+   IAED +  PGL   VS+
Sbjct: 574 NGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQ 633

Query: 493 G------------------------------------------YSEKCISYAESHDQSIV 552
           G                                          Y++K +SYAE+H+QSI 
Sbjct: 634 GGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSIS 693

Query: 553 GDKTIAFLLMDKEMYSGMSCFTDASP----VVERGIALHKMIHFLTMALGGEGYLNFMGN 612
           G ++ A +L     + G+    + SP    +++RGI+LHKMI  +T   GG  YLNFMGN
Sbjct: 694 GGRSFAEIL-----FGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFMGN 753

Query: 613 EVN--------LVSNSADQSLG------LSLGIPSGLIPREGNGWSYDKCR--RQWNLPD 672
           E            SN+   SL       L  G+   L   +      DK +      LP 
Sbjct: 754 EFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLFSFDKELMDLDKSKGILSRGLPS 813

Query: 673 TDHLR--YKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDSDAWDFGGHGRV 692
             H+     VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD   +GG G V
Sbjct: 814 IHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKYGGQGIV 873

BLAST of HG10004270 vs. TAIR 10
Match: AT3G20440.3 (Alpha amylase family protein )

HSP 1 Score: 319.3 bits (817), Expect = 8.5e-87
Identity = 196/567 (34.57%), Postives = 292/567 (51.50%), Query Frame = 0

Query: 193 PAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRF----------------GAPYEF 252
           PA+ H S+ +  F   +G  ++R+PAW  Y     T F                 A Y++
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATYVQPGMTAFEDEGKQAYAIHWEPSPEAAYKW 393

Query: 253 KHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFA-DLFTRIKENNYNTVQLMAIMEHSYY 312
           K+ +P  P++ R+YE HVG+S SEP+VS++ EF   +   +K   YN +QL+ + EH  Y
Sbjct: 394 KYSKPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDY 453

Query: 313 ASFGYHITNFFAVSSRSGTPEDLK-ILCKAHGLGLRVLMDVVHSHASNNVTDGLNGFDVG 372
            + GY +TNFFA SSR GTP+D K ++ +AHGLGL V +D+VHS+A+ +   GL+ FD  
Sbjct: 454 FTVGYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD-- 513

Query: 373 QSTQDSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSML 432
             + D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EYQ DG++F  + SM+
Sbjct: 514 -GSNDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMI 573

Query: 433 YHHHGINMAFTGNIMRF-SEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGMPGLGR- 492
           Y H+G   +F  ++  + ++  D DA++YL+LAN + H   P+   IAED +  PGL   
Sbjct: 574 YTHNGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEP 633

Query: 493 VSRG------------------------------------------YSEKCISYAESHDQ 552
           VS+G                                          Y++K +SYAE+H+Q
Sbjct: 634 VSQGGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQ 693

Query: 553 SIVGDKTIAFLLMDKEMYSGMSCFTDASP----VVERGIALHKMIHFLTMALGGEGYLNF 612
           SI G ++ A +L     + G+    + SP    +++RGI+LHKMI  +T   GG  YLNF
Sbjct: 694 SISGGRSFAEIL-----FGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNF 753

Query: 613 MGNEVNLVSNSADQSLGLSLGIPSGL-IPREGNGWSYDKCRRQWN--------------- 659
           MGNE                G P  +  P + N +S+    R+W+               
Sbjct: 754 MGNE---------------FGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLFSFDK 813

BLAST of HG10004270 vs. TAIR 10
Match: AT3G20440.1 (Alpha amylase family protein )

HSP 1 Score: 291.6 bits (745), Expect = 1.9e-78
Identity = 196/579 (33.85%), Postives = 289/579 (49.91%), Query Frame = 0

Query: 193 PAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTRFG-------------APYEFKHP 252
           PA+ H S+ +  F   +G  ++R+PAW  Y  V P   G             A Y++K+ 
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYS 393

Query: 253 RPAKPKAPRVYEAHVGMSSSEPRVSSYREFADLFTRIKENNYNTVQLMAIMEHSYYASFG 312
           +P  P++ R+YE HVG+S SEP+VS++ EF                              
Sbjct: 394 KPKVPESLRIYECHVGISGSEPKVSTFEEFTK---------------------------- 453

Query: 313 YHITNFFAVSSRSGTPEDLK-ILCKAHGLGLRVLMDVVHSHASNNVTDGLNGFDVGQSTQ 372
             +TNFFA SSR GTP+D K ++ +AHGLGL V +D+VHS+A+ +   GL+ FD    + 
Sbjct: 454 -KVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD---GSN 513

Query: 373 DSYFHTGDRGYHKRWDSRLFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHH 432
           D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EYQ DG++F  + SM+Y H+
Sbjct: 514 DCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHN 573

Query: 433 GINMAFTGNIMRF-SEATDVDAVVYLMLANNLTHSILPDATVIAEDVSGMPGLGR-VSRG 492
           G   +F  ++  + ++  D DA++YL+LAN + H   P+   IAED +  PGL   VS+G
Sbjct: 574 GF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQG 633

Query: 493 ------------------------------------------YSEKCISYAESHDQSIVG 552
                                                     Y++K +SYAE+H+QSI G
Sbjct: 634 GLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSISG 693

Query: 553 DKTIAFLLMDKEMYSGMSCFTDASP----VVERGIALHKMIHFLTMALGGEGYLNFMGNE 612
            ++ A +L     + G+    + SP    +++RGI+LHKMI  +T   GG  YLNFMGNE
Sbjct: 694 GRSFAEIL-----FGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFMGNE 753

Query: 613 VN--------LVSNSADQSLG------LSLGIPSGLIPREGNGWSYDKCR--RQWNLPDT 672
                       SN+   SL       L  G+   L   +      DK +      LP  
Sbjct: 754 FGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLFSFDKELMDLDKSKGILSRGLPSI 813

Query: 673 DHLR--YKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDSDAWDFGGHGRVG 692
            H+     VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD   +GG G V 
Sbjct: 814 HHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKYGGQGIV- 861