HG10004189 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10004189
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionamino acid transporter AVT1C-like
LocationChr08: 14597048 .. 14603877 (+)
RNA-Seq ExpressionHG10004189
SyntenyHG10004189
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAACTCTGTGTCGGATCGTAGTTTTTACATCGAAAGCTCTGATGATGAAGATTTGGAGAAGGAAATCGATAATGACGATGGAAACGACTCAGATTCTTCCGGTTCATCGGTGGATAATCGGAATCATAATCAACCAAGTTCTTACACTACCGCCGCTTGGCCTCAAAGTTACAGGTTCACTTTCCCTTCCTTTCAACTCTTTCCTTCTTCCTTCTTCTGTATTCATAATCAATTCAAAGATTTTCTGGTAAAAATTAATTTCAGGTTCAATTATAATTTCCTCCAATTTCGTTTCTACAATTTAGGCCCTTCCGCTTCTATATTTTAGGTCCAGTTGGATAAGAATTTTGTTTTTAGTTTTTAGTTTCTTAAAATTAAGGTTATAAACACTTTAGTTTTAAATTTATTAGTTTGTTATCTATTTTTTAACAATATTTTAAAAAATAAAATAAAATTTTAGTTAAGTATTACTAAATATGTAAATAGTGATAGACAATTAAGAGAAAATAGATTTAATTTTTAAAAACTAAAAGTTAAATAATGAGTCTTAAAAGTTAGAATTGAATCGGTAAAGAATTTAGTGTAGTTTAATAAAATTCCATAAATAGTCTTTATAATTGGATAAAATTGTAAAATTACTCTATATGATATGAAAAAAAGTTAGAAATTAATCTCTATAATTTGATTAAACTTCATAAATTTATTGTAACCTTTTAAGTTTATCACTCATAATAATAACAATAATAATTCTTATTATTATTTGTTTTTGTAAAAGTATTAGGTGCCTCTCGAATAATACATATATTTGTATATCCCATCAAATTATCTAAACATAATTGGTCAGATGGTAAATTTTTTTTTTTTTTTTTTTTTAAAATATTTTAACTTATTTTTTATATGTATGATGAGAGTAAGATATATAAAAATAAATGTTAGTGGAATCCAGTAAGGAATTTGAATAAGGAAATAATCATAATTTTGATTATGGATAACTTTTGGGGTTGTGAGGATATTGTCCTGATTATTCTGGAGAGATGCCCCACACACGAGTCGTCACACCCTCCACCTAATAAAGTATGCCTTTTTTTTGTTCAAAAGTGATTTTTGTTACCTAAGATAGACATTGTAAAACTGAATCATTTGTAAATATCAATACAATAATTTTCTTTTGGGAGTCCTAAAATATTTATAATTTTAAAATTAAAAATAATTATTGGTCAAAGTATAAATATAATATTTCTGATTTTAAATTAGATTCACAATATGTAATAAACAATATAATCTTCTCTCTTTTTTCCGACCCAGTTTAGTGGCCAAGCACACTTTGCCATGTGTTAATTTGTTTATTTTTTATTATAATAAATGATTATTAGAAATAATTATTAAAATTTTAGTTTTGAAAATTAAACTTATAAATACGATTTTCACTCATAAATTTTTATATTTTACTATCCATCATCGAGTAATTATTTTCAAATTCAAAGTCAAGTTTTGAAATTAAAAAAAAAATCAAAAACTTATTTTTGACTCGAGTTTGAGTTTTTTTTTTTTTTTAAGAAATATGAAACTCATAATTGAAAATTTGAAATGGAAAGGAAACAATCATAAATATTGAAAAAAACAAAAAAATGAAATTGTTAGCAAATAAGATGAACTTTAACTTTTTGGGATAGGGATTTGAACTTTCAACCTCAAAGATGGAGTTCATGTGAATTATTAACTAAATCAAACTTACTTTGACTATTATTATTATTATTATTATTATTAAGAGGTTGTTTGGACCCCAATCTCAAAGAGGTAGAGTGGGCTATTATATCTCACTCCATGTTTAGGACTCCAATTATAATAACTTATGTTTTCAACTATTATAATTTACAATTAATTAAAATATTATTATTTAAATTTTTTTTTTTTACCTTGTTTATAATTTCTATACTGTTTTCTACCCAAACCTAAATACACACTATTGTAACTTAAACTAAAATAATCTGCACTCAAACACGACTATTATAACTATAATAACTAAACCAATTTAGCTCCCTTAGTGTCTCCTAAATGTTTGTAGCCAGAAATCTAAATTACTTTTAATAAAAGTTCTTTAAAGGGAAAAGGTTTGGATGTGTAAATGAATGATGCTTACAACCAGCAACAATCAATATAAGTCTAAATTTTTTAGTTAGTAATAATATTGGCTGTTTTAAGGAGATATTGACAATGTGCATATTGAATTTGAAATCAAATTAATAATGTTGCAGGCAATCAATTGATATGCTGGGAAGTGTACATTCACCAAGTATTGGGCTTTTAGGAACTCCATCATTAGCCAGATTTGGAAGCTCATTTCTTTCTTCCTCACTAACAAGAAGATTCACTCCAGAAGCATTCTCTTCTGCCATTTCTAAACCTCTGCTCCCTACAGTAATAGATGAGCCCCAAAAACATACCCCTTATTCTCATTTCGGCCCTCCACTTCCTTCCCGGCGCTCTTCTTTATCCGTCAGGAGGGATGACAAAGATAAGCCCGTCATTGATTCTCATGGCCTTCCCATTTCTCGCCATAGTACTTTTGGCCAAGCTGTTGTTAATGGTAAATATTAATTCATTACTTAAAACACACCATCTCCTTCGTCTTCTAATACTACTTTGATTCTTTACTTTTGACTTTTGTTCATTTTGGTCCTTATACTTTGAATTTTCATTTATTTTGAGTCCTATACTTTCAAGACTTTGATTCATTTTAGTCTTCGAACTTTTAAAAAATAACAATTTCGGTCCCTTAAAAATGATATAAAAATTAAAATGAAACTTACCTTGACATTGACCAAGCTGCTTTTGGTCTTTATACTCTTCGCTTTTGTTCATTTTGAGTTTTATACTTTAAATTTTAGTTCAATTTGGTCTTTATACTTTCAAAATGTTCATTTTCATCGTTGAACTTTAAAAAAATGGTCATTTTGGTCGCTTAAGAATGGTATAAAAATGAATGTACATCACAAAGTACATGAATGAAAATAAAACAAAGTTAAAAGTATAGGGATCAAAATGAAAATAGTAAAAGGTATAAGAATGAAAATGAAACAAAGTGAAAAGTATAGGGAGTAAAGCAGTATTTAAACTATTATAATTATTATTGTGAAAACTGAATGATGGAAGAAGTTTGATTGTAACGTAGGGATCAATGTGCTGTGTGGGGTGGGAATACTTTCGACTCCATATGCAATGAAAGAAGGAGGATGGATGGGAATATCGATATTGTTGATATTTGCAGTGCTTTCATTCTACACTGGAATACTCCTGCGAGCTTGTTTGGATAGCAGACCTGGCCTTGAAACTTACCCTGACATTGGCCAAGCTGCCTTTGGTGCCATGGGCCGTGTCGTCATCTCGGTAACTTCCTTTATACACCATATAAATTTCTCGATACAAAATTAATACTCGTGTTTAAAGAAATTTAGTCAATGTACTGTGTTCAAATTGAGATTTAAAAAATTAATACAAAGGGACAAATATATGGTGAATTTTTAAAAATAGAAAAATAACGTAAACTATTTACGTAAAATAACAAAATTTTTAGATCGTTGTAATAAACGTTAATAGAAGTCTATCAGGTTTTCTTAGTTATACTCAATTTTTCACTAAATACTTATTTTTTGCCATTAATATTAATGTCTATTAATTAATTTAAAAGAATTAAACGAATTATAATGAATTAAGTTTCACTATTAAAATTAAAATTTTATTTCATAATTATTTTAAATTAATTAATAGATATTAACACCAAAAACTGAAAATGAGCATTTATTGAAAAAATCGAGTTTAACAAGTGTAAATCTTGAAACTTAGACCAAATTCAAACATAACTCAATTCTTGTAACATTTTGAAATTTAGAAACTAAATTGAAATCAAATTTAAATCTTAAGACCAAAGGTGTAACATATTGAAACTTATGAATCAAATGAAAACTAAACTCAAAATTTAGAAACAAAAATAAAAAATATATATATATATGTAAAGTGTAGCTAGGACAAAACTTCACGGGAAATGAACTAATATTTGCTTTAAATTTTTAGAGGGTATTTAAACACTGAATTGATTATTATAGTTAGTAGAGTGTTGCTATTTTAGTTTAGATAAAAAATAATAAAGAGAGAATGAGAGAATGGGTTGTAAATAGTAAACAATATAACTATTTAAAATAGTAATAGTGAACCAAACGTCCCTTTATACTTTCTTTTCCTTGCGGAATTTCTTTCGTTTAACATTTTGTTTTGATTTCTTGTGGATGCAGATCGTATTATACGTGGAATTATACGTAAGTACTACTTTTTGTCTCCGGCTTTTAATTATCATTATTTTATTGGATAATGGTTAACGTAAGGGCCCCACCTACATTTTATATCCTGCATCACACACCAAGTGGCAAGTCTCTTGTCCGCCAGCGCTCTTTTATGACTTAGGAACCACATTTTGATTTTGAGTCTCTTTCTGCGTCCGTGGTGGAAAAGAGCCCAAGTTTTGAAGAAATAGTGTTGGATAATTCCACTGTATAACATTTTAAAGGTTAATAATGATGGGTAACATTTTAAAGGTTAATAATGATGGGTAACTAACATTTTTTTTAGAAAGGGTAAATATAACGAAATCTATTCGTAATAAACTTAAGACTTTTATAATGATAGAGTCTATCACTAATAGATGTTAAGTGCTGAATTCAAATTTTACTAAAAATCTGTAATTTTTTTTTATATTGTATCATATTTACTAAGTTTGATTATTATATTTCCAACTTCTCTTACCTCTATTTGTCCAACGATTTTAATTTAAATAATGATTTTCTTTCTAATTTTGTCAACGAAAAGAAGAAAATAAGACTGGAAAATATAGAAGCAATGATCAAAACTCTCTTTTGCATATGATGCTTGATGTCTTCAGCAGATGCTTATCAAGTGTTTGAGAAAATGACTGGGAGAAAATATGGAGCCTAACCACCCATGTTCTGTGGGATTTTGTAGCGTAGCTACTTTCGACCTTTGGCTAATTGAATAGGCAAAACGGCTCTTCTTTTTTCTTTTTTGAAAAAGTGGAACTTAGCTCAGAAGCAAGAACCCTTGTCATCCTCTTTTTCAGTCTTGGCTGTTTCTGTTCCACAAATCCATTTATTGTCATTCTTTATTCAGATTACCCTCGTCCTTTACACTCACTCGTTCTTCTCATTCTCAATAGGTAAAATCTCTAGCAATATTAGTGAATTTAAACCAGAAAACAATTTTAACCTGAAAATTTTGATATCATGTGGAAGCTCTGGCAAATGAAACGGACTCCTCCCCACTGGGCCAATAAACATGTAGTGAAAACTGAAAAGCTAAAGCTTATTTTTTTTTGTTTCTAGTTTAAATAAAACGTGGAAAGGGATCTGCATTATGACCCAAGTCAGTATTTTTCATTATGATTTTGTCTGCTAATCATGCAACGTGCTTTTTCATTTTCCTAATGATTTTGAAACAACAAATTAAGCCTTATGTTGACAAAGTGACCCAACTAGACATGCTAGTCTTGCAGGCTTCTTGTATTGAGTACTTAATTCTGGAGAGCGACAATTTGTCTACATTGTTTCCCCCTGCGCACATAAGTTTTGGCGGATTCGAAATAAATGCACACCTTTTATTCGCTATAGCAACTGCTCTTGCTGTTCTTCCGACTGTTTATCTTCGGGATCTCAGTATTTTAAGTTATATCTCTGGTAAGCTGGCTAGATTTATCGTGCCAATATATATGTATATGTTTATGAGTTTTATTGGTTGCCTCGCATCATACACCAATTCAGGCTTATTTATTATGTTTTACAAGTTTTGAATAGTGCCTAAGTAACTTGCATTCACTTTGTACTACTAATGTCTAACGCATTCCTATTATGCTAACAAGTGAATGACATGCGTCCAACGACTATCAGAATGCTCAAGTATCAAAGACACATTACTCGAACTAAAGTGTCTGTTTGCTTCTTTGCACGAGACGGATGTTGCTGTCAAAAATGATTTTTCTTATTGTTCTTGTTCTTAAAAAATGCCCAAAAAAATAGCTATGAATGGTTTTGCAAGGTGACTCCACTGAACCTCTCAAGTCTCCCAGTTGCTCTGGGTCTGTACGGTTTCTGCTACTCAGGACATGCTGTATTTCCCAACATATATAGCTCAATGGGTAAACAGAGCCAATTCCCAGCAGTCCTTTTGACATGGTAATGTGAAATCTTCATCCATTTTCACAAACAATTTGTCTTGTTCATCTCCTACTGTCTGCTTCTGCATAGAGCTATTAATTTGATTTAAATATGCATCATACTTCTAATGTATTAATCATGTAAGCCAAAGTGAGTTTAACTCAACGGTAATTGACATGACCTTTCTCCTTAGAAATCGGAGGTTCGATCTCTCATCCCTGCAGTTGTGGAACTATATATGTGTATATATATCTATATATCTCGACACTCATTTATTTAGTATAATGTAAAGACAAAAAGGGAACCGACTTTGTCAATTAAGAAATCTTTGTGTTGCATGATATGTCTGTTTGTCACCCTTTTGAATGGAGAGGTGATAATTTCCTGCCAACTATTAAAAAAAATAATCATTATCTAAGATGTTTATTATATTAATAGCATTTAATTTTCTTAAAAATGCAGTTTTGGCATTTGTACTTTGATGTATGCTGGAGTCGCTATCATGGGATACTTCATGTTTGGAGAGTCGACATTATCCCAGTACACTCTTAACCTGCCCCAGGATTTGGTTGCTTCAAAGATTGCTGTGTGGACTACGGTACGGTGA

mRNA sequence

ATGAAGAACTCTGTGTCGGATCGTAGTTTTTACATCGAAAGCTCTGATGATGAAGATTTGGAGAAGGAAATCGATAATGACGATGGAAACGACTCAGATTCTTCCGGTTCATCGGTGGATAATCGGAATCATAATCAACCAAGTTCTTACACTACCGCCGCTTGGCCTCAAAGTTACAGGCAATCAATTGATATGCTGGGAAGTGTACATTCACCAAGTATTGGGCTTTTAGGAACTCCATCATTAGCCAGATTTGGAAGCTCATTTCTTTCTTCCTCACTAACAAGAAGATTCACTCCAGAAGCATTCTCTTCTGCCATTTCTAAACCTCTGCTCCCTACAGTAATAGATGAGCCCCAAAAACATACCCCTTATTCTCATTTCGGCCCTCCACTTCCTTCCCGGCGCTCTTCTTTATCCGTCAGGAGGGATGACAAAGATAAGCCCGTCATTGATTCTCATGGCCTTCCCATTTCTCGCCATAGTACTTTTGGCCAAGCTGTTGTTAATGGGATCAATGTGCTGTGTGGGGTGGGAATACTTTCGACTCCATATGCAATGAAAGAAGGAGGATGGATGGGAATATCGATATTGTTGATATTTGCAGTGCTTTCATTCTACACTGGAATACTCCTGCGAGCTTGTTTGGATAGCAGACCTGGCCTTGAAACTTACCCTGACATTGGCCAAGCTGCCTTTGGTGCCATGGGCCGTGTCGTCATCTCGGCTTCTTGTATTGAGTACTTAATTCTGGAGAGCGACAATTTGTCTACATTGTTTCCCCCTGCGCACATAAGTTTTGGCGGATTCGAAATAAATGCACACCTTTTATTCGCTATAGCAACTGCTCTTGCTGTTCTTCCGACTGTTTATCTTCGGGATCTCAGTATTTTAAGTTATATCTCTGGACATGCTGTATTTCCCAACATATATAGCTCAATGGGTAAACAGAGCCAATTCCCAGCAGTCCTTTTGACATGTTTTGGCATTTGTACTTTGATGTATGCTGGAGTCGCTATCATGGGATACTTCATGTTTGGAGAGTCGACATTATCCCAGTACACTCTTAACCTGCCCCAGGATTTGGTTGCTTCAAAGATTGCTGTGTGGACTACGGTACGGTGA

Coding sequence (CDS)

ATGAAGAACTCTGTGTCGGATCGTAGTTTTTACATCGAAAGCTCTGATGATGAAGATTTGGAGAAGGAAATCGATAATGACGATGGAAACGACTCAGATTCTTCCGGTTCATCGGTGGATAATCGGAATCATAATCAACCAAGTTCTTACACTACCGCCGCTTGGCCTCAAAGTTACAGGCAATCAATTGATATGCTGGGAAGTGTACATTCACCAAGTATTGGGCTTTTAGGAACTCCATCATTAGCCAGATTTGGAAGCTCATTTCTTTCTTCCTCACTAACAAGAAGATTCACTCCAGAAGCATTCTCTTCTGCCATTTCTAAACCTCTGCTCCCTACAGTAATAGATGAGCCCCAAAAACATACCCCTTATTCTCATTTCGGCCCTCCACTTCCTTCCCGGCGCTCTTCTTTATCCGTCAGGAGGGATGACAAAGATAAGCCCGTCATTGATTCTCATGGCCTTCCCATTTCTCGCCATAGTACTTTTGGCCAAGCTGTTGTTAATGGGATCAATGTGCTGTGTGGGGTGGGAATACTTTCGACTCCATATGCAATGAAAGAAGGAGGATGGATGGGAATATCGATATTGTTGATATTTGCAGTGCTTTCATTCTACACTGGAATACTCCTGCGAGCTTGTTTGGATAGCAGACCTGGCCTTGAAACTTACCCTGACATTGGCCAAGCTGCCTTTGGTGCCATGGGCCGTGTCGTCATCTCGGCTTCTTGTATTGAGTACTTAATTCTGGAGAGCGACAATTTGTCTACATTGTTTCCCCCTGCGCACATAAGTTTTGGCGGATTCGAAATAAATGCACACCTTTTATTCGCTATAGCAACTGCTCTTGCTGTTCTTCCGACTGTTTATCTTCGGGATCTCAGTATTTTAAGTTATATCTCTGGACATGCTGTATTTCCCAACATATATAGCTCAATGGGTAAACAGAGCCAATTCCCAGCAGTCCTTTTGACATGTTTTGGCATTTGTACTTTGATGTATGCTGGAGTCGCTATCATGGGATACTTCATGTTTGGAGAGTCGACATTATCCCAGTACACTCTTAACCTGCCCCAGGATTTGGTTGCTTCAAAGATTGCTGTGTGGACTACGGTACGGTGA

Protein sequence

MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYRQSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQKHTPYSHFGPPLPSRRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVVISASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTVYLRDLSILSYISGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLPQDLVASKIAVWTTVR
Homology
BLAST of HG10004189 vs. NCBI nr
Match: XP_038886658.1 (amino acid transporter AVT1C-like [Benincasa hispida] >XP_038886659.1 amino acid transporter AVT1C-like [Benincasa hispida])

HSP 1 Score: 656.0 bits (1691), Expect = 2.0e-184
Identity = 354/430 (82.33%), Postives = 360/430 (83.72%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYR 60
           MKNSVSDRSFYIESSDDEDLEKEIDNDDG+DSDSS SS DNRNHNQPSSY TAAWPQSYR
Sbjct: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGSDSDSSSSSADNRNHNQPSSYNTAAWPQSYR 60

Query: 61  QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQ 120
           QSIDMLGS+HSPSIGLLGT SLARFGSSFLSSSLTRRFTPE FSS+ISKPLLPTVIDEPQ
Sbjct: 61  QSIDMLGSLHSPSIGLLGTSSLARFGSSFLSSSLTRRFTPEVFSSSISKPLLPTVIDEPQ 120

Query: 121 KHTPYSHFGPPLPSRRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGI 180
           KH  YSH GP LPSRRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGI
Sbjct: 121 KHPSYSHLGPSLPSRRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGI 180

Query: 181 LSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVV 240
           LSTPYAMKEGGW+GISIL+IFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRV 
Sbjct: 181 LSTPYAMKEGGWLGISILMIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVA 240

Query: 241 IS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTVYL 300
           IS        ASCIEY+ILESDNLSTLFP AHISFGG EINAHLLFAIATALAVLPTVYL
Sbjct: 241 ISIVLYVELYASCIEYVILESDNLSTLFPRAHISFGGLEINAHLLFAIATALAVLPTVYL 300

Query: 301 RDLSILSYI-------------------------------------------------SG 360
           RDLSILSYI                                                 SG
Sbjct: 301 RDLSILSYISAGGVVASIVVVLCLFWVGFVDDVGFHGKVTPLNLSSLPVALGLYGFCYSG 360

Query: 361 HAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLPQDLV 374
           HAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGY MFGESTLSQYTLNLPQDLV
Sbjct: 361 HAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYLMFGESTLSQYTLNLPQDLV 420

BLAST of HG10004189 vs. NCBI nr
Match: XP_008456512.1 (PREDICTED: vacuolar amino acid transporter 1-like [Cucumis melo])

HSP 1 Score: 646.0 bits (1665), Expect = 2.0e-181
Identity = 348/432 (80.56%), Postives = 364/432 (84.26%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYR 60
           MKNSVSDRSFYIESSDDEDLEKEIDNDDGN+SDSSGSS DN+NHNQPSSYTTAAWPQSYR
Sbjct: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNESDSSGSSADNQNHNQPSSYTTAAWPQSYR 60

Query: 61  QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQ 120
           QSIDMLGSVHSPSIGLLGT SLARFGSSFLSSSLTRRFTPEAFSS+ISKPLLPTVID+P 
Sbjct: 61  QSIDMLGSVHSPSIGLLGTSSLARFGSSFLSSSLTRRFTPEAFSSSISKPLLPTVIDDPH 120

Query: 121 KHT-PYSHFGPPLPSRRSSLSVRRDDKDKP-VIDSHGLPISRHSTFGQAVVNGINVLCGV 180
           KH  P+S  GPPLPSRRSS+S+RRDDKDKP VIDSHGLP+SRHSTFGQAVVNGINVLCGV
Sbjct: 121 KHAPPHSPLGPPLPSRRSSISIRRDDKDKPVVIDSHGLPVSRHSTFGQAVVNGINVLCGV 180

Query: 181 GILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR 240
           GILSTPYAMKEGGW+G+SIL+IFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR
Sbjct: 181 GILSTPYAMKEGGWLGLSILMIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR 240

Query: 241 VVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTV 300
           V+IS        ASCIEY+ILESDNLS+LFP AHISFGG EINAHLLFAIATALAVLPTV
Sbjct: 241 VIISIVLYVELYASCIEYVILESDNLSSLFPRAHISFGGLEINAHLLFAIATALAVLPTV 300

Query: 301 YLRDLSILSYI------------------------------------------------- 360
           YLRDLSILSYI                                                 
Sbjct: 301 YLRDLSILSYISAGGVVASIVVVLCLFWVGLVDDVGFHGNVTPLNLSSLPVALGLYGFCY 360

Query: 361 SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLPQD 374
           SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTL+YAGVA+MGY MFGESTLSQYTLNLPQD
Sbjct: 361 SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLLYAGVAVMGYLMFGESTLSQYTLNLPQD 420

BLAST of HG10004189 vs. NCBI nr
Match: KAA0036488.1 (vacuolar amino acid transporter 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 641.0 bits (1652), Expect = 6.6e-180
Identity = 347/441 (78.68%), Postives = 363/441 (82.31%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSY- 60
           MKNSVSDRSFYIESSDDEDLEKEIDNDDGN+SDSSGSS DN+NHNQPSSYTTAAWPQSY 
Sbjct: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNESDSSGSSADNQNHNQPSSYTTAAWPQSYR 60

Query: 61  -----------------RQSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEA 120
                            RQSIDMLGSVHSPSIGLLGT SLARFGSSFLSSSLTRRFTPEA
Sbjct: 61  FAFSSSSQLLPSFSIFLRQSIDMLGSVHSPSIGLLGTSSLARFGSSFLSSSLTRRFTPEA 120

Query: 121 FSSAISKPLLPTVIDEPQKHT-PYSHFGPPLPSRRSSLSVRRDDKDKP-VIDSHGLPISR 180
           FSS+ISKPLLPTVID+P KH  P+S  GPPLPSRRSS+S+RRDDKDKP VIDSHGLP+SR
Sbjct: 121 FSSSISKPLLPTVIDDPHKHAPPHSPLGPPLPSRRSSISIRRDDKDKPVVIDSHGLPVSR 180

Query: 181 HSTFGQAVVNGINVLCGVGILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRP 240
           HSTFGQAVVNGINVLCGVGILSTPYAMKEGGW+G+SIL+IFAVLSFYTGILLRACLDSRP
Sbjct: 181 HSTFGQAVVNGINVLCGVGILSTPYAMKEGGWLGLSILMIFAVLSFYTGILLRACLDSRP 240

Query: 241 GLETYPDIGQAAFGAMGRVVISASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAI 300
           GLETYPDIGQAAFGAMGRV+ISASCIEY+ILESDNLS+LFP AHISFGG EINAHLLFAI
Sbjct: 241 GLETYPDIGQAAFGAMGRVIISASCIEYVILESDNLSSLFPRAHISFGGLEINAHLLFAI 300

Query: 301 ATALAVLPTVYLRDLSILSYI--------------------------------------- 360
           ATALAVLPTVYLRDLSILSYI                                       
Sbjct: 301 ATALAVLPTVYLRDLSILSYISAGGVVASIVVVLCLFWVGLVDDVGFHGNVTPLNLSSLP 360

Query: 361 ----------SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTL 373
                     SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTL+YAGVA+MGY MFGESTL
Sbjct: 361 VALGLYGFCYSGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLLYAGVAVMGYLMFGESTL 420

BLAST of HG10004189 vs. NCBI nr
Match: XP_004149900.1 (amino acid transporter AVT1C [Cucumis sativus] >KAE8647305.1 hypothetical protein Csa_002895 [Cucumis sativus])

HSP 1 Score: 640.2 bits (1650), Expect = 1.1e-179
Identity = 348/432 (80.56%), Postives = 360/432 (83.33%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYR 60
           MKNSVSDRSFYIESSDDEDLEKEIDN+DGN+SDSSG S DN+NHNQPSSY TAAWPQSYR
Sbjct: 1   MKNSVSDRSFYIESSDDEDLEKEIDNEDGNESDSSGLSADNQNHNQPSSYNTAAWPQSYR 60

Query: 61  QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQ 120
           QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSS+ISKPLLPTVID+P 
Sbjct: 61  QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSSISKPLLPTVIDDPH 120

Query: 121 KHT-PYSHFGPPLPSRRSSLSVRRDDKDKP-VIDSHGLPISRHSTFGQAVVNGINVLCGV 180
           KH  P+S  GP LPSRRSS+SVRRDDKDKP VIDSHGLPISRHSTFGQAVVNGINVLCGV
Sbjct: 121 KHAPPHSPLGPSLPSRRSSISVRRDDKDKPVVIDSHGLPISRHSTFGQAVVNGINVLCGV 180

Query: 181 GILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR 240
           GILSTPYAMKEGGWMG+SIL+IFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR
Sbjct: 181 GILSTPYAMKEGGWMGLSILMIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR 240

Query: 241 VVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTV 300
           V IS        ASCIEY+ILESDNLS+LFP AHISFGG EINAHLLFAIATALAVLPTV
Sbjct: 241 VAISIVLYVELYASCIEYVILESDNLSSLFPRAHISFGGLEINAHLLFAIATALAVLPTV 300

Query: 301 YLRDLSILSYI------------------------------------------------- 360
           YLRDLSILSYI                                                 
Sbjct: 301 YLRDLSILSYISAGGVVASIVVVLCLFWVGLVDDVGFHGKVTPLNLSSLPVALGLYGFCY 360

Query: 361 SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLPQD 374
           SGHAVFPNIYSSMG QSQFPAVLLTCFGICTLMYAGVA+MGY MFGESTLSQYTLNLPQD
Sbjct: 361 SGHAVFPNIYSSMGNQSQFPAVLLTCFGICTLMYAGVAVMGYLMFGESTLSQYTLNLPQD 420

BLAST of HG10004189 vs. NCBI nr
Match: TYJ99999.1 (vacuolar amino acid transporter 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 633.6 bits (1633), Expect = 1.0e-177
Identity = 347/449 (77.28%), Postives = 363/449 (80.85%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSY- 60
           MKNSVSDRSFYIESSDDEDLEKEIDNDDGN+SDSSGSS DN+NHNQPSSYTTAAWPQSY 
Sbjct: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNESDSSGSSADNQNHNQPSSYTTAAWPQSYR 60

Query: 61  -----------------RQSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEA 120
                            RQSIDMLGSVHSPSIGLLGT SLARFGSSFLSSSLTRRFTPEA
Sbjct: 61  FAFSSSSQLLPSFSIFLRQSIDMLGSVHSPSIGLLGTSSLARFGSSFLSSSLTRRFTPEA 120

Query: 121 FSSAISKPLLPTVIDEPQKHT-PYSHFGPPLPSRRSSLSVRRDDKDKP-VIDSHGLPISR 180
           FSS+ISKPLLPTVID+P KH  P+S  GPPLPSRRSS+S+RRDDKDKP VIDSHGLP+SR
Sbjct: 121 FSSSISKPLLPTVIDDPHKHAPPHSPLGPPLPSRRSSISIRRDDKDKPVVIDSHGLPVSR 180

Query: 181 HSTFGQAVVNGINVLCGVGILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRP 240
           HSTFGQAVVNGINVLCGVGILSTPYAMKEGGW+G+SIL+IFAVLSFYTGILLRACLDSRP
Sbjct: 181 HSTFGQAVVNGINVLCGVGILSTPYAMKEGGWLGLSILMIFAVLSFYTGILLRACLDSRP 240

Query: 241 GLETYPDIGQAAFGAMGRVVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEI 300
           GLETYPDIGQAAFGAMGRV+IS        ASCIEY+ILESDNLS+LFP AHISFGG EI
Sbjct: 241 GLETYPDIGQAAFGAMGRVIISIVLYVELYASCIEYVILESDNLSSLFPRAHISFGGLEI 300

Query: 301 NAHLLFAIATALAVLPTVYLRDLSILSYI------------------------------- 360
           NAHLLFAIATALAVLPTVYLRDLSILSYI                               
Sbjct: 301 NAHLLFAIATALAVLPTVYLRDLSILSYISAGGVVASIVVVLCLFWVGLVDDVGFHGNVT 360

Query: 361 ------------------SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGY 373
                             SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTL+YAGVA+MGY
Sbjct: 361 PLNLSSLPVALGLYGFCYSGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLLYAGVAVMGY 420

BLAST of HG10004189 vs. ExPASy Swiss-Prot
Match: F4IUW3 (Amino acid transporter AVT1C OS=Arabidopsis thaliana OX=3702 GN=AVT1C PE=1 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.3e-117
Identity = 244/434 (56.22%), Postives = 296/434 (68.20%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVD-NRNHNQPSSYTTAAWPQSY 60
           M +  SD+SFYIES D++D +  ++ D G+ SDSS    D N+ H +PSSYTT AWPQSY
Sbjct: 1   MNHVPSDQSFYIESEDEDDRKDYVEEDGGSHSDSSDDVYDENQAHIKPSSYTT-AWPQSY 60

Query: 61  RQSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDE- 120
           RQSID+  SV SP IG LG  S+ RFGSSFLSS L RR TPE+  + ++KPLL    DE 
Sbjct: 61  RQSIDLYSSVPSPGIGFLGNNSMTRFGSSFLSSGLIRRHTPESLPT-VTKPLLEEQADEQ 120

Query: 121 --PQKHTPYSHFGPPLPSRRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLC 180
             P+          P+PSRR S+  R+D+K   V  SH +P+SR+S++GQAV+NG+NVLC
Sbjct: 121 ALPKHRLSSQGLLSPIPSRRGSM--RKDEKSSMV--SHEIPMSRNSSYGQAVLNGLNVLC 180

Query: 181 GVGILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAM 240
           GVGILSTPYA KEGGW+G+ IL ++ +LSFYTGILLR CLDS   LETYPDIGQAAFG  
Sbjct: 181 GVGILSTPYAAKEGGWLGLMILFVYGLLSFYTGILLRYCLDSESDLETYPDIGQAAFGTT 240

Query: 241 GRVVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLP 300
           GR+ +S        A C+EY+ILESDNLS+L+P A +S GGF+++A  LFA+ T LAVLP
Sbjct: 241 GRIFVSIVLYLELYACCVEYIILESDNLSSLYPNAALSIGGFQLDARHLFALLTTLAVLP 300

Query: 301 TVYLRDLSILSYI----------------------------------------------- 360
           TV+LRDLS+LSYI                                               
Sbjct: 301 TVWLRDLSVLSYISAGGVIASVLVVLCLFWIGLVDEVGIHSKGTTLNLSTLPVAIGLYGY 360

Query: 361 --SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLP 374
             SGHAVFPNIY+SM K SQ+PAVLLTCFGICTLMYAGVA+MGY MFGEST SQ+TLNLP
Sbjct: 361 CYSGHAVFPNIYTSMAKPSQYPAVLLTCFGICTLMYAGVAVMGYTMFGESTQSQFTLNLP 420

BLAST of HG10004189 vs. ExPASy Swiss-Prot
Match: F4JE35 (Amino acid transporter AVT1B OS=Arabidopsis thaliana OX=3702 GN=AVT1B PE=3 SV=2)

HSP 1 Score: 382.9 bits (982), Expect = 4.2e-105
Identity = 226/433 (52.19%), Postives = 282/433 (65.13%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEI---DNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQ 60
           M +S SD+S YIES D +D  K +   ++DDG  SD+S +   N++H   +S  + AWP+
Sbjct: 1   MNHSTSDQSLYIESDDGDDERKHLSDDEDDDGTLSDTSDAYNQNQHHLSKASPYSTAWPK 60

Query: 61  SYRQSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVID 120
           SYRQSIDM GSV SP++G L   S++R GSSF+SS+LTRR TPE+    ++KPLL    +
Sbjct: 61  SYRQSIDMFGSVPSPNLGFLANSSMSRRGSSFMSSTLTRRHTPESL-PCVTKPLLED--E 120

Query: 121 EPQKHTPYSHFGPPLPSRRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCG 180
           E  KH   +H    LPS+ SS           ++ SH + IS  S+FGQAV+NG+NVLCG
Sbjct: 121 EAPKHKLSTH--SLLPSKPSS----------SMVVSHDMGISNDSSFGQAVLNGVNVLCG 180

Query: 181 VGILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMG 240
           VGILSTPYA+KEGGW+G+ IL  F +L FYTG+LLR CLDS P ++TYPDIG AAFG+ G
Sbjct: 181 VGILSTPYAVKEGGWLGLIILFAFGILCFYTGLLLRYCLDSHPDVQTYPDIGHAAFGSTG 240

Query: 241 RVVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPT 300
           R+++S        A  +EY+ILE DNLS++FP A +S GGF ++A  LFA+ T LAVLPT
Sbjct: 241 RILVSVILYMELYAMSVEYIILEGDNLSSMFPNASLSIGGFHLDAPRLFALLTTLAVLPT 300

Query: 301 VYLRDLSILSYI------------------------------------------------ 360
           V+LRDLS+LSYI                                                
Sbjct: 301 VWLRDLSVLSYISAGGVIASVLVVLCLFWVGLVDDVGIHSKGTPLNLATLPVSVGLYGYC 360

Query: 361 -SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLPQ 374
            SGH VFPNIY+SM K SQF AVLL  FGICTLMYAGVA+MGY MFGEST SQ+TLNLPQ
Sbjct: 361 YSGHGVFPNIYTSMAKPSQFSAVLLASFGICTLMYAGVAVMGYSMFGESTESQFTLNLPQ 418

BLAST of HG10004189 vs. ExPASy Swiss-Prot
Match: Q8GYS4 (Amino acid transporter AVT1D OS=Arabidopsis thaliana OX=3702 GN=AVT1D PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 4.4e-70
Identity = 166/418 (39.71%), Postives = 228/418 (54.55%), Query Frame = 0

Query: 15  SDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYRQSIDMLGSVHSPSI 74
           SDDE    +     G +SDSS     +R+ N  S  +   WPQSYRQS+D+L  +  PS+
Sbjct: 29  SDDEHDGDDDGRRGGENSDSSSPLSRDRSDNNLSDVSNPPWPQSYRQSMDLLTGMTPPSV 88

Query: 75  GLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQKHTPYSHFGPPLPS 134
             +   S  R  SSF      +    ++ SS+ SKPLL                  P+P 
Sbjct: 89  SFMPQSSSRRLASSFQKKQ--QSSFCDSLSSSSSKPLL----------------SQPVPD 148

Query: 135 RRSS-LSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGILSTPYAMKEGGWM 194
           +  + L V    + K  +    LP     +F Q+V+NG NVLCG+G+++ PYA+KE GW+
Sbjct: 149 KEETILPVNPQSQLKLSVTDLPLPEPNLCSFSQSVLNGTNVLCGLGLITMPYAIKESGWL 208

Query: 195 GISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVVIS--------ASC 254
           G+ ILL F V++ YTG+L++ CL+S PG++TYPDIGQAAFG  GR +IS        A+C
Sbjct: 209 GLPILLFFGVITCYTGVLMKRCLESSPGIQTYPDIGQAAFGITGRFIISILLYVELYAAC 268

Query: 255 IEYLILESDNLSTLFPPAHISF-GGFEINAHLLFAIATALAVLPTVYLRDLSILSYI--- 314
           +EY+I+ SDNLS LFP   +S   G  +++  +FAI T L VLPTV+L+DLS+LSY+   
Sbjct: 269 VEYIIMMSDNLSGLFPNVSLSIASGISLDSPQIFAILTTLLVLPTVWLKDLSLLSYLSVG 328

Query: 315 ----------------------------------------------SGHAVFPNIYSSMG 374
                                                         SGH+VFPNIYSSM 
Sbjct: 329 GVLASILLGICLFWVGAVDGIGFHATGRVFDLSNLPVTIGIFGFGYSGHSVFPNIYSSMK 388

BLAST of HG10004189 vs. ExPASy Swiss-Prot
Match: Q1PER9 (Amino acid transporter AVT1G OS=Arabidopsis thaliana OX=3702 GN=AVT1G PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 7.3e-65
Identity = 165/416 (39.66%), Postives = 225/416 (54.09%), Query Frame = 0

Query: 15  SDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYRQSIDMLGSVHSPSI 74
           +DDE+ + ++  DD +D  SS SS+        S   +A WP+SYRQS+D+L  V  P+I
Sbjct: 14  TDDEECQVDVSCDDEDDDSSSYSSL--------SQNDSAPWPRSYRQSVDILTGVTPPTI 73

Query: 75  GLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQKHTPYSHFGPPLPS 134
             +   S     +S ++S   RR T  A S A S    P + ++             +  
Sbjct: 74  SFIHRRSSQTSFTSSVASLYKRRPTSIANSFASSTSKQPLLSEKDD-----------VLF 133

Query: 135 RRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGILSTPYAMKEGGWMG 194
             S + +   D       S+G P     +F Q+V+NGINVLCG+ +L+ PYA+KEGGW+G
Sbjct: 134 LSSQIGLSNTDL------SYGEP--NFCSFPQSVLNGINVLCGISLLTMPYAVKEGGWLG 193

Query: 195 ISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVVIS--------ASCI 254
           + ILL FA+++ YTGILL+ CL+S   L TYPDIGQAAFG  GR++IS          C+
Sbjct: 194 LCILLSFAIITCYTGILLKRCLESSSDLRTYPDIGQAAFGFTGRLIISILLYMELYVCCV 253

Query: 255 EYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTVYLRDLSILSYI----- 314
           EY+I+ SDNLS +FP   ++  G  +++  +FAI+  L VLPTV+L+DLS+LSY+     
Sbjct: 254 EYIIMMSDNLSRVFPNITLNIVGVSLDSPQIFAISATLIVLPTVWLKDLSLLSYLSAGGV 313

Query: 315 --------------------------------------------SGHAVFPNIYSSMGKQ 374
                                                       SGHAV P+IYSSM + 
Sbjct: 314 FVSILLALCLFWVGSVDGVGFHTGGKSLDLANLPVAIGIFGFGFSGHAVLPSIYSSMKEP 373

BLAST of HG10004189 vs. ExPASy Swiss-Prot
Match: Q8LPF4 (Amino acid transporter AVT1E OS=Arabidopsis thaliana OX=3702 GN=AVT1E PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.7e-64
Identity = 162/415 (39.04%), Postives = 221/415 (53.25%), Query Frame = 0

Query: 18  EDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYRQSIDMLGSVHSPSIGLL 77
           EDL    D +D  +S +  S          S      WP+SYRQS+D+L  V  P+    
Sbjct: 12  EDLYHTFDEED-EESQTESSVPSTPLSRNRSEDVPVPWPRSYRQSMDLLTGVTPPT---- 71

Query: 78  GTPSLARFGSSFLSSSLTRRFTP--EAFSSAISKPLLPTVIDEPQKHTPYSHFGPPLPSR 137
                    +SF+SS   RR +    +F+S+ SK  L  +ID+ +  +          + 
Sbjct: 72  --------STSFVSSFRQRRQSSVFGSFTSSPSKQQL--LIDKDEIQSSVVSSIKSFLAS 131

Query: 138 RSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGILSTPYAMKEGGWMGI 197
              LSV  D              +R  TF Q+V+NGINVLCGV +L+ PYA+KEGGW+G+
Sbjct: 132 HLQLSVPGDLLTPQE--------NRSCTFSQSVLNGINVLCGVALLTMPYAVKEGGWLGL 191

Query: 198 SILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVVIS--------ASCIE 257
            IL  F +++FYTGILL+ CL++ PG+ TYPDIGQAAFG  GR+++S        ASC+E
Sbjct: 192 FILFSFGIITFYTGILLKRCLENSPGIHTYPDIGQAAFGTTGRILVSILLYVELYASCVE 251

Query: 258 YLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTVYLRDLSILSYIS----- 317
           Y+I+ SDNLS +FP   +   GF +++  +FAI T L VLPTV+L+DLS+LSY+S     
Sbjct: 252 YIIMMSDNLSRMFPNTSLYINGFSLDSTQVFAITTTLIVLPTVWLKDLSLLSYLSAGGVI 311

Query: 318 --------------------------------------------GHAVFPNIYSSMGKQS 374
                                                        H+VFPNIYSSM + S
Sbjct: 312 SSILLALCLFWAGSVDGVGFHISGQALDITNIPVAIGIYGFGFGSHSVFPNIYSSMKEPS 371

BLAST of HG10004189 vs. ExPASy TrEMBL
Match: A0A1S3C3I3 (vacuolar amino acid transporter 1-like OS=Cucumis melo OX=3656 GN=LOC103496440 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 9.9e-182
Identity = 348/432 (80.56%), Postives = 364/432 (84.26%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYR 60
           MKNSVSDRSFYIESSDDEDLEKEIDNDDGN+SDSSGSS DN+NHNQPSSYTTAAWPQSYR
Sbjct: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNESDSSGSSADNQNHNQPSSYTTAAWPQSYR 60

Query: 61  QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQ 120
           QSIDMLGSVHSPSIGLLGT SLARFGSSFLSSSLTRRFTPEAFSS+ISKPLLPTVID+P 
Sbjct: 61  QSIDMLGSVHSPSIGLLGTSSLARFGSSFLSSSLTRRFTPEAFSSSISKPLLPTVIDDPH 120

Query: 121 KHT-PYSHFGPPLPSRRSSLSVRRDDKDKP-VIDSHGLPISRHSTFGQAVVNGINVLCGV 180
           KH  P+S  GPPLPSRRSS+S+RRDDKDKP VIDSHGLP+SRHSTFGQAVVNGINVLCGV
Sbjct: 121 KHAPPHSPLGPPLPSRRSSISIRRDDKDKPVVIDSHGLPVSRHSTFGQAVVNGINVLCGV 180

Query: 181 GILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR 240
           GILSTPYAMKEGGW+G+SIL+IFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR
Sbjct: 181 GILSTPYAMKEGGWLGLSILMIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR 240

Query: 241 VVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTV 300
           V+IS        ASCIEY+ILESDNLS+LFP AHISFGG EINAHLLFAIATALAVLPTV
Sbjct: 241 VIISIVLYVELYASCIEYVILESDNLSSLFPRAHISFGGLEINAHLLFAIATALAVLPTV 300

Query: 301 YLRDLSILSYI------------------------------------------------- 360
           YLRDLSILSYI                                                 
Sbjct: 301 YLRDLSILSYISAGGVVASIVVVLCLFWVGLVDDVGFHGNVTPLNLSSLPVALGLYGFCY 360

Query: 361 SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLPQD 374
           SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTL+YAGVA+MGY MFGESTLSQYTLNLPQD
Sbjct: 361 SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLLYAGVAVMGYLMFGESTLSQYTLNLPQD 420

BLAST of HG10004189 vs. ExPASy TrEMBL
Match: A0A5A7T108 (Vacuolar amino acid transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold147G00570 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 3.2e-180
Identity = 347/441 (78.68%), Postives = 363/441 (82.31%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSY- 60
           MKNSVSDRSFYIESSDDEDLEKEIDNDDGN+SDSSGSS DN+NHNQPSSYTTAAWPQSY 
Sbjct: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNESDSSGSSADNQNHNQPSSYTTAAWPQSYR 60

Query: 61  -----------------RQSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEA 120
                            RQSIDMLGSVHSPSIGLLGT SLARFGSSFLSSSLTRRFTPEA
Sbjct: 61  FAFSSSSQLLPSFSIFLRQSIDMLGSVHSPSIGLLGTSSLARFGSSFLSSSLTRRFTPEA 120

Query: 121 FSSAISKPLLPTVIDEPQKHT-PYSHFGPPLPSRRSSLSVRRDDKDKP-VIDSHGLPISR 180
           FSS+ISKPLLPTVID+P KH  P+S  GPPLPSRRSS+S+RRDDKDKP VIDSHGLP+SR
Sbjct: 121 FSSSISKPLLPTVIDDPHKHAPPHSPLGPPLPSRRSSISIRRDDKDKPVVIDSHGLPVSR 180

Query: 181 HSTFGQAVVNGINVLCGVGILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRP 240
           HSTFGQAVVNGINVLCGVGILSTPYAMKEGGW+G+SIL+IFAVLSFYTGILLRACLDSRP
Sbjct: 181 HSTFGQAVVNGINVLCGVGILSTPYAMKEGGWLGLSILMIFAVLSFYTGILLRACLDSRP 240

Query: 241 GLETYPDIGQAAFGAMGRVVISASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAI 300
           GLETYPDIGQAAFGAMGRV+ISASCIEY+ILESDNLS+LFP AHISFGG EINAHLLFAI
Sbjct: 241 GLETYPDIGQAAFGAMGRVIISASCIEYVILESDNLSSLFPRAHISFGGLEINAHLLFAI 300

Query: 301 ATALAVLPTVYLRDLSILSYI--------------------------------------- 360
           ATALAVLPTVYLRDLSILSYI                                       
Sbjct: 301 ATALAVLPTVYLRDLSILSYISAGGVVASIVVVLCLFWVGLVDDVGFHGNVTPLNLSSLP 360

Query: 361 ----------SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTL 373
                     SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTL+YAGVA+MGY MFGESTL
Sbjct: 361 VALGLYGFCYSGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLLYAGVAVMGYLMFGESTL 420

BLAST of HG10004189 vs. ExPASy TrEMBL
Match: A0A0A0KG55 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G406020 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 5.4e-180
Identity = 348/432 (80.56%), Postives = 360/432 (83.33%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYR 60
           MKNSVSDRSFYIESSDDEDLEKEIDN+DGN+SDSSG S DN+NHNQPSSY TAAWPQSYR
Sbjct: 1   MKNSVSDRSFYIESSDDEDLEKEIDNEDGNESDSSGLSADNQNHNQPSSYNTAAWPQSYR 60

Query: 61  QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQ 120
           QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSS+ISKPLLPTVID+P 
Sbjct: 61  QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSSISKPLLPTVIDDPH 120

Query: 121 KHT-PYSHFGPPLPSRRSSLSVRRDDKDKP-VIDSHGLPISRHSTFGQAVVNGINVLCGV 180
           KH  P+S  GP LPSRRSS+SVRRDDKDKP VIDSHGLPISRHSTFGQAVVNGINVLCGV
Sbjct: 121 KHAPPHSPLGPSLPSRRSSISVRRDDKDKPVVIDSHGLPISRHSTFGQAVVNGINVLCGV 180

Query: 181 GILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR 240
           GILSTPYAMKEGGWMG+SIL+IFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR
Sbjct: 181 GILSTPYAMKEGGWMGLSILMIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGR 240

Query: 241 VVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTV 300
           V IS        ASCIEY+ILESDNLS+LFP AHISFGG EINAHLLFAIATALAVLPTV
Sbjct: 241 VAISIVLYVELYASCIEYVILESDNLSSLFPRAHISFGGLEINAHLLFAIATALAVLPTV 300

Query: 301 YLRDLSILSYI------------------------------------------------- 360
           YLRDLSILSYI                                                 
Sbjct: 301 YLRDLSILSYISAGGVVASIVVVLCLFWVGLVDDVGFHGKVTPLNLSSLPVALGLYGFCY 360

Query: 361 SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLPQD 374
           SGHAVFPNIYSSMG QSQFPAVLLTCFGICTLMYAGVA+MGY MFGESTLSQYTLNLPQD
Sbjct: 361 SGHAVFPNIYSSMGNQSQFPAVLLTCFGICTLMYAGVAVMGYLMFGESTLSQYTLNLPQD 420

BLAST of HG10004189 vs. ExPASy TrEMBL
Match: A0A5D3BLY2 (Vacuolar amino acid transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00450 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 5.1e-178
Identity = 347/449 (77.28%), Postives = 363/449 (80.85%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSY- 60
           MKNSVSDRSFYIESSDDEDLEKEIDNDDGN+SDSSGSS DN+NHNQPSSYTTAAWPQSY 
Sbjct: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNESDSSGSSADNQNHNQPSSYTTAAWPQSYR 60

Query: 61  -----------------RQSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEA 120
                            RQSIDMLGSVHSPSIGLLGT SLARFGSSFLSSSLTRRFTPEA
Sbjct: 61  FAFSSSSQLLPSFSIFLRQSIDMLGSVHSPSIGLLGTSSLARFGSSFLSSSLTRRFTPEA 120

Query: 121 FSSAISKPLLPTVIDEPQKHT-PYSHFGPPLPSRRSSLSVRRDDKDKP-VIDSHGLPISR 180
           FSS+ISKPLLPTVID+P KH  P+S  GPPLPSRRSS+S+RRDDKDKP VIDSHGLP+SR
Sbjct: 121 FSSSISKPLLPTVIDDPHKHAPPHSPLGPPLPSRRSSISIRRDDKDKPVVIDSHGLPVSR 180

Query: 181 HSTFGQAVVNGINVLCGVGILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRP 240
           HSTFGQAVVNGINVLCGVGILSTPYAMKEGGW+G+SIL+IFAVLSFYTGILLRACLDSRP
Sbjct: 181 HSTFGQAVVNGINVLCGVGILSTPYAMKEGGWLGLSILMIFAVLSFYTGILLRACLDSRP 240

Query: 241 GLETYPDIGQAAFGAMGRVVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEI 300
           GLETYPDIGQAAFGAMGRV+IS        ASCIEY+ILESDNLS+LFP AHISFGG EI
Sbjct: 241 GLETYPDIGQAAFGAMGRVIISIVLYVELYASCIEYVILESDNLSSLFPRAHISFGGLEI 300

Query: 301 NAHLLFAIATALAVLPTVYLRDLSILSYI------------------------------- 360
           NAHLLFAIATALAVLPTVYLRDLSILSYI                               
Sbjct: 301 NAHLLFAIATALAVLPTVYLRDLSILSYISAGGVVASIVVVLCLFWVGLVDDVGFHGNVT 360

Query: 361 ------------------SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGY 373
                             SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTL+YAGVA+MGY
Sbjct: 361 PLNLSSLPVALGLYGFCYSGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLLYAGVAVMGY 420

BLAST of HG10004189 vs. ExPASy TrEMBL
Match: A0A6J1K167 (amino acid transporter AVT1C-like OS=Cucurbita maxima OX=3661 GN=LOC111489216 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.8e-176
Identity = 339/430 (78.84%), Postives = 358/430 (83.26%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYR 60
           MKNSVSDRSFYIESSDDED EKEI  DDGN+S+SSGSSVDNRN NQPSSY  AAWPQSYR
Sbjct: 1   MKNSVSDRSFYIESSDDEDFEKEI--DDGNESESSGSSVDNRNINQPSSYNAAAWPQSYR 60

Query: 61  QSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQ 120
           QSIDMLGS+HSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSS+ISKPLLPTVIDE Q
Sbjct: 61  QSIDMLGSLHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSSISKPLLPTVIDEQQ 120

Query: 121 KHTPYSHFGPPLPSRRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGI 180
           K+ PYS  GP LPSRRSS+SVRRDDKDKPV+DSHGLPISRH +FGQAVVNGINVLCGVGI
Sbjct: 121 KYAPYSPLGPQLPSRRSSISVRRDDKDKPVVDSHGLPISRHCSFGQAVVNGINVLCGVGI 180

Query: 181 LSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVV 240
           LSTPYAM++GGW+G+SILLIFAVLSFYTGILLRACLDS+PGLETYPDIGQAAFGA+GRV 
Sbjct: 181 LSTPYAMQQGGWLGLSILLIFAVLSFYTGILLRACLDSKPGLETYPDIGQAAFGAIGRVA 240

Query: 241 IS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTVYL 300
           IS        ASCIEY+ILESDNLSTLFP AHISFGGFE++AHLLFAIATALAVLPTV+L
Sbjct: 241 ISIVLYVELYASCIEYVILESDNLSTLFPRAHISFGGFELSAHLLFAIATALAVLPTVWL 300

Query: 301 RDLSILSYI-------------------------------------------------SG 360
           RDLSILSYI                                                 SG
Sbjct: 301 RDLSILSYISAGGVIASIVVVICLFWVGLVDDVGFHGTVTPLKLSTLPVALGLYGFCYSG 360

Query: 361 HAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLPQDLV 374
           HAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAI+GY MFGESTLSQYTLNLPQDLV
Sbjct: 361 HAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIIGYLMFGESTLSQYTLNLPQDLV 420

BLAST of HG10004189 vs. TAIR 10
Match: AT2G39130.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 424.5 bits (1090), Expect = 9.0e-119
Identity = 244/434 (56.22%), Postives = 296/434 (68.20%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEIDNDDGNDSDSSGSSVD-NRNHNQPSSYTTAAWPQSY 60
           M +  SD+SFYIES D++D +  ++ D G+ SDSS    D N+ H +PSSYTT AWPQSY
Sbjct: 1   MNHVPSDQSFYIESEDEDDRKDYVEEDGGSHSDSSDDVYDENQAHIKPSSYTT-AWPQSY 60

Query: 61  RQSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDE- 120
           RQSID+  SV SP IG LG  S+ RFGSSFLSS L RR TPE+  + ++KPLL    DE 
Sbjct: 61  RQSIDLYSSVPSPGIGFLGNNSMTRFGSSFLSSGLIRRHTPESLPT-VTKPLLEEQADEQ 120

Query: 121 --PQKHTPYSHFGPPLPSRRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLC 180
             P+          P+PSRR S+  R+D+K   V  SH +P+SR+S++GQAV+NG+NVLC
Sbjct: 121 ALPKHRLSSQGLLSPIPSRRGSM--RKDEKSSMV--SHEIPMSRNSSYGQAVLNGLNVLC 180

Query: 181 GVGILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAM 240
           GVGILSTPYA KEGGW+G+ IL ++ +LSFYTGILLR CLDS   LETYPDIGQAAFG  
Sbjct: 181 GVGILSTPYAAKEGGWLGLMILFVYGLLSFYTGILLRYCLDSESDLETYPDIGQAAFGTT 240

Query: 241 GRVVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLP 300
           GR+ +S        A C+EY+ILESDNLS+L+P A +S GGF+++A  LFA+ T LAVLP
Sbjct: 241 GRIFVSIVLYLELYACCVEYIILESDNLSSLYPNAALSIGGFQLDARHLFALLTTLAVLP 300

Query: 301 TVYLRDLSILSYI----------------------------------------------- 360
           TV+LRDLS+LSYI                                               
Sbjct: 301 TVWLRDLSVLSYISAGGVIASVLVVLCLFWIGLVDEVGIHSKGTTLNLSTLPVAIGLYGY 360

Query: 361 --SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLP 374
             SGHAVFPNIY+SM K SQ+PAVLLTCFGICTLMYAGVA+MGY MFGEST SQ+TLNLP
Sbjct: 361 CYSGHAVFPNIYTSMAKPSQYPAVLLTCFGICTLMYAGVAVMGYTMFGESTQSQFTLNLP 420

BLAST of HG10004189 vs. TAIR 10
Match: AT3G54830.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 382.9 bits (982), Expect = 3.0e-106
Identity = 226/433 (52.19%), Postives = 282/433 (65.13%), Query Frame = 0

Query: 1   MKNSVSDRSFYIESSDDEDLEKEI---DNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQ 60
           M +S SD+S YIES D +D  K +   ++DDG  SD+S +   N++H   +S  + AWP+
Sbjct: 1   MNHSTSDQSLYIESDDGDDERKHLSDDEDDDGTLSDTSDAYNQNQHHLSKASPYSTAWPK 60

Query: 61  SYRQSIDMLGSVHSPSIGLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVID 120
           SYRQSIDM GSV SP++G L   S++R GSSF+SS+LTRR TPE+    ++KPLL    +
Sbjct: 61  SYRQSIDMFGSVPSPNLGFLANSSMSRRGSSFMSSTLTRRHTPESL-PCVTKPLLED--E 120

Query: 121 EPQKHTPYSHFGPPLPSRRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCG 180
           E  KH   +H    LPS+ SS           ++ SH + IS  S+FGQAV+NG+NVLCG
Sbjct: 121 EAPKHKLSTH--SLLPSKPSS----------SMVVSHDMGISNDSSFGQAVLNGVNVLCG 180

Query: 181 VGILSTPYAMKEGGWMGISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMG 240
           VGILSTPYA+KEGGW+G+ IL  F +L FYTG+LLR CLDS P ++TYPDIG AAFG+ G
Sbjct: 181 VGILSTPYAVKEGGWLGLIILFAFGILCFYTGLLLRYCLDSHPDVQTYPDIGHAAFGSTG 240

Query: 241 RVVIS--------ASCIEYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPT 300
           R+++S        A  +EY+ILE DNLS++FP A +S GGF ++A  LFA+ T LAVLPT
Sbjct: 241 RILVSVILYMELYAMSVEYIILEGDNLSSMFPNASLSIGGFHLDAPRLFALLTTLAVLPT 300

Query: 301 VYLRDLSILSYI------------------------------------------------ 360
           V+LRDLS+LSYI                                                
Sbjct: 301 VWLRDLSVLSYISAGGVIASVLVVLCLFWVGLVDDVGIHSKGTPLNLATLPVSVGLYGYC 360

Query: 361 -SGHAVFPNIYSSMGKQSQFPAVLLTCFGICTLMYAGVAIMGYFMFGESTLSQYTLNLPQ 374
            SGH VFPNIY+SM K SQF AVLL  FGICTLMYAGVA+MGY MFGEST SQ+TLNLPQ
Sbjct: 361 YSGHGVFPNIYTSMAKPSQFSAVLLASFGICTLMYAGVAVMGYSMFGESTESQFTLNLPQ 418

BLAST of HG10004189 vs. TAIR 10
Match: AT5G02180.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 266.5 bits (680), Expect = 3.1e-71
Identity = 166/418 (39.71%), Postives = 228/418 (54.55%), Query Frame = 0

Query: 15  SDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYRQSIDMLGSVHSPSI 74
           SDDE    +     G +SDSS     +R+ N  S  +   WPQSYRQS+D+L  +  PS+
Sbjct: 29  SDDEHDGDDDGRRGGENSDSSSPLSRDRSDNNLSDVSNPPWPQSYRQSMDLLTGMTPPSV 88

Query: 75  GLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQKHTPYSHFGPPLPS 134
             +   S  R  SSF      +    ++ SS+ SKPLL                  P+P 
Sbjct: 89  SFMPQSSSRRLASSFQKKQ--QSSFCDSLSSSSSKPLL----------------SQPVPD 148

Query: 135 RRSS-LSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGILSTPYAMKEGGWM 194
           +  + L V    + K  +    LP     +F Q+V+NG NVLCG+G+++ PYA+KE GW+
Sbjct: 149 KEETILPVNPQSQLKLSVTDLPLPEPNLCSFSQSVLNGTNVLCGLGLITMPYAIKESGWL 208

Query: 195 GISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVVIS--------ASC 254
           G+ ILL F V++ YTG+L++ CL+S PG++TYPDIGQAAFG  GR +IS        A+C
Sbjct: 209 GLPILLFFGVITCYTGVLMKRCLESSPGIQTYPDIGQAAFGITGRFIISILLYVELYAAC 268

Query: 255 IEYLILESDNLSTLFPPAHISF-GGFEINAHLLFAIATALAVLPTVYLRDLSILSYI--- 314
           +EY+I+ SDNLS LFP   +S   G  +++  +FAI T L VLPTV+L+DLS+LSY+   
Sbjct: 269 VEYIIMMSDNLSGLFPNVSLSIASGISLDSPQIFAILTTLLVLPTVWLKDLSLLSYLSVG 328

Query: 315 ----------------------------------------------SGHAVFPNIYSSMG 374
                                                         SGH+VFPNIYSSM 
Sbjct: 329 GVLASILLGICLFWVGAVDGIGFHATGRVFDLSNLPVTIGIFGFGYSGHSVFPNIYSSMK 388

BLAST of HG10004189 vs. TAIR 10
Match: AT3G09330.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 249.2 bits (635), Expect = 5.2e-66
Identity = 165/416 (39.66%), Postives = 225/416 (54.09%), Query Frame = 0

Query: 15  SDDEDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYRQSIDMLGSVHSPSI 74
           +DDE+ + ++  DD +D  SS SS+        S   +A WP+SYRQS+D+L  V  P+I
Sbjct: 14  TDDEECQVDVSCDDEDDDSSSYSSL--------SQNDSAPWPRSYRQSVDILTGVTPPTI 73

Query: 75  GLLGTPSLARFGSSFLSSSLTRRFTPEAFSSAISKPLLPTVIDEPQKHTPYSHFGPPLPS 134
             +   S     +S ++S   RR T  A S A S    P + ++             +  
Sbjct: 74  SFIHRRSSQTSFTSSVASLYKRRPTSIANSFASSTSKQPLLSEKDD-----------VLF 133

Query: 135 RRSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGILSTPYAMKEGGWMG 194
             S + +   D       S+G P     +F Q+V+NGINVLCG+ +L+ PYA+KEGGW+G
Sbjct: 134 LSSQIGLSNTDL------SYGEP--NFCSFPQSVLNGINVLCGISLLTMPYAVKEGGWLG 193

Query: 195 ISILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVVIS--------ASCI 254
           + ILL FA+++ YTGILL+ CL+S   L TYPDIGQAAFG  GR++IS          C+
Sbjct: 194 LCILLSFAIITCYTGILLKRCLESSSDLRTYPDIGQAAFGFTGRLIISILLYMELYVCCV 253

Query: 255 EYLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTVYLRDLSILSYI----- 314
           EY+I+ SDNLS +FP   ++  G  +++  +FAI+  L VLPTV+L+DLS+LSY+     
Sbjct: 254 EYIIMMSDNLSRVFPNITLNIVGVSLDSPQIFAISATLIVLPTVWLKDLSLLSYLSAGGV 313

Query: 315 --------------------------------------------SGHAVFPNIYSSMGKQ 374
                                                       SGHAV P+IYSSM + 
Sbjct: 314 FVSILLALCLFWVGSVDGVGFHTGGKSLDLANLPVAIGIFGFGFSGHAVLPSIYSSMKEP 373

BLAST of HG10004189 vs. TAIR 10
Match: AT5G02170.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 246.5 bits (628), Expect = 3.4e-65
Identity = 162/415 (39.04%), Postives = 221/415 (53.25%), Query Frame = 0

Query: 18  EDLEKEIDNDDGNDSDSSGSSVDNRNHNQPSSYTTAAWPQSYRQSIDMLGSVHSPSIGLL 77
           EDL    D +D  +S +  S          S      WP+SYRQS+D+L  V  P+    
Sbjct: 12  EDLYHTFDEED-EESQTESSVPSTPLSRNRSEDVPVPWPRSYRQSMDLLTGVTPPT---- 71

Query: 78  GTPSLARFGSSFLSSSLTRRFTP--EAFSSAISKPLLPTVIDEPQKHTPYSHFGPPLPSR 137
                    +SF+SS   RR +    +F+S+ SK  L  +ID+ +  +          + 
Sbjct: 72  --------STSFVSSFRQRRQSSVFGSFTSSPSKQQL--LIDKDEIQSSVVSSIKSFLAS 131

Query: 138 RSSLSVRRDDKDKPVIDSHGLPISRHSTFGQAVVNGINVLCGVGILSTPYAMKEGGWMGI 197
              LSV  D              +R  TF Q+V+NGINVLCGV +L+ PYA+KEGGW+G+
Sbjct: 132 HLQLSVPGDLLTPQE--------NRSCTFSQSVLNGINVLCGVALLTMPYAVKEGGWLGL 191

Query: 198 SILLIFAVLSFYTGILLRACLDSRPGLETYPDIGQAAFGAMGRVVIS--------ASCIE 257
            IL  F +++FYTGILL+ CL++ PG+ TYPDIGQAAFG  GR+++S        ASC+E
Sbjct: 192 FILFSFGIITFYTGILLKRCLENSPGIHTYPDIGQAAFGTTGRILVSILLYVELYASCVE 251

Query: 258 YLILESDNLSTLFPPAHISFGGFEINAHLLFAIATALAVLPTVYLRDLSILSYIS----- 317
           Y+I+ SDNLS +FP   +   GF +++  +FAI T L VLPTV+L+DLS+LSY+S     
Sbjct: 252 YIIMMSDNLSRMFPNTSLYINGFSLDSTQVFAITTTLIVLPTVWLKDLSLLSYLSAGGVI 311

Query: 318 --------------------------------------------GHAVFPNIYSSMGKQS 374
                                                        H+VFPNIYSSM + S
Sbjct: 312 SSILLALCLFWAGSVDGVGFHISGQALDITNIPVAIGIYGFGFGSHSVFPNIYSSMKEPS 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886658.12.0e-18482.33amino acid transporter AVT1C-like [Benincasa hispida] >XP_038886659.1 amino acid... [more]
XP_008456512.12.0e-18180.56PREDICTED: vacuolar amino acid transporter 1-like [Cucumis melo][more]
KAA0036488.16.6e-18078.68vacuolar amino acid transporter 1-like [Cucumis melo var. makuwa][more]
XP_004149900.11.1e-17980.56amino acid transporter AVT1C [Cucumis sativus] >KAE8647305.1 hypothetical protei... [more]
TYJ99999.11.0e-17777.28vacuolar amino acid transporter 1-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
F4IUW31.3e-11756.22Amino acid transporter AVT1C OS=Arabidopsis thaliana OX=3702 GN=AVT1C PE=1 SV=1[more]
F4JE354.2e-10552.19Amino acid transporter AVT1B OS=Arabidopsis thaliana OX=3702 GN=AVT1B PE=3 SV=2[more]
Q8GYS44.4e-7039.71Amino acid transporter AVT1D OS=Arabidopsis thaliana OX=3702 GN=AVT1D PE=2 SV=1[more]
Q1PER97.3e-6539.66Amino acid transporter AVT1G OS=Arabidopsis thaliana OX=3702 GN=AVT1G PE=2 SV=1[more]
Q8LPF44.7e-6439.04Amino acid transporter AVT1E OS=Arabidopsis thaliana OX=3702 GN=AVT1E PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3C3I39.9e-18280.56vacuolar amino acid transporter 1-like OS=Cucumis melo OX=3656 GN=LOC103496440 P... [more]
A0A5A7T1083.2e-18078.68Vacuolar amino acid transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A0A0KG555.4e-18080.56Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G406020 PE... [more]
A0A5D3BLY25.1e-17877.28Vacuolar amino acid transporter 1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1K1672.8e-17678.84amino acid transporter AVT1C-like OS=Cucurbita maxima OX=3661 GN=LOC111489216 PE... [more]
Match NameE-valueIdentityDescription
AT2G39130.19.0e-11956.22Transmembrane amino acid transporter family protein [more]
AT3G54830.13.0e-10652.19Transmembrane amino acid transporter family protein [more]
AT5G02180.13.1e-7139.71Transmembrane amino acid transporter family protein [more]
AT3G09330.15.2e-6639.66Transmembrane amino acid transporter family protein [more]
AT5G02170.13.4e-6539.04Transmembrane amino acid transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013057Amino acid transporter, transmembrane domainPFAMPF01490Aa_transcoord: 161..302
e-value: 4.9E-21
score: 74.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..53
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..53
NoneNo IPR availablePANTHERPTHR48017OS05G0424000 PROTEIN-RELATEDcoord: 28..302
coord: 303..372
NoneNo IPR availablePANTHERPTHR48017:SF126TRANSMEMBRANE AMINO ACID TRANSPORTER FAMILY PROTEIN-RELATEDcoord: 28..302
coord: 303..372

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10004189.1HG10004189.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006865 amino acid transport
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015293 symporter activity