HG10003825 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003825
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Non-specific serine/threonine protein kinase
Location	Chr08: 9836922 .. 9839965 (+)
RNA-Seq Expression	HG10003825
Synteny	HG10003825

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGCAGCATGAAGTCCATATACCCAGACGAGATTCTATTCTCACATACGATGTTGCTGAAGGTGATGATCATGGATCTGAAAGCGAGCATTCAGAGTGGTCTTTTGCTTCCTTGAGTAATGAACAAGAATCCCAAACTACTGATAGGGATATAATGATGGTAATTCTTTCGTCACTTTCTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGTGTCTATTCCCTATAATTTGTTATTTTAAATCTAATCCCATGTGTCAACGGGTGTGATTTTTTTTATTATTAGGTTCATCTGCTCCATCTTGCATGTGCCCCTAAAGGACCGTTGGCTGATGCTCTGCCAAAATTGGCATCTGAACTTTACAATTTGGGGGTATGTTCAATAATTATTCCTTACCCACGTCCTTTCTCAGTCCTAATCTTTATCTACTTGATCGTCAGGTCCTGTCAAAAGTGGCTCTGGATTTAGCTTCTAAACCACCTTCCATGTTTCATAAAAAATTTGAGACTGCTTTCCAGGAACAGATGGTACTTTGTAATTCTTTCTATTTAAATTACTTTCATACTTCTGCCTTTCTGTTCTTTCTTGTAATTCAACTCTAACATTTTCTTTCTTCCACATTTTGTTTTACCTAGAATGCTACTAGTTTCTCCCAGTTTTGGAATCCTTCTGATTTTGGTGGCCCAACATCTTCTCAACTGAGTTCTCGGTATCTTAACGATTTTGAGGAGCTAAAGCCCCTTGGTAATGTGTTCTGGCGTTTTGCATGTGCATGTGTTATTAATGTTCAATTTGTAATAATGTTGATGGTTTCTCTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATATATATATATATATATGTATGTGTGTATATATGTATATATATATAGTTTTGTTAAGAGGCTACATAATCATAGAATTCTTTTTAGTCTCATTGGATGGGTCTCTTTTTTTTTTTTTTTGAAAAGGAAACGAGTCTCTTCATTGAATTAGTGAAAGGAGACTAATGCTCAAGTTACAAATAATGATACAAGAGCTCAAAACCTATGGATCAGCGAATGCACCCGGACATCTCAACTAGGTTGACACCCCCTTAGCACTCTCATCATATCCGAACAAAAAGAAAAAAAAAATCAAAATGTCCAAGGCCAATATGCAGTCCAACATAACAAAATACTCCCCGAATACAAAGAAAACAACTAGAAATTGCAACTTGATAAACCCTTTTTAGTCTCATTGGATGAGTCTCTTATTTATATGATTTCATTTTAGCAATGAAATTCATTTGGGGAAAAAAAGACGAGAAAAGATTCATTCAAAAGAGGCAAACCATGTACCTTGGGGGTGAAGGCACAAGGAGTCCCTCCCCCAACAAAAATACAAAAAGACCTTCTATTCTATTTTTTAAAATTTTTTAAATTCAATTCAATTTATTAATTTGTTTTTCTTAACGAGAGATGAAAGTTTACATTGAAGTGAATAAATGAAAAGAGACTAATGAGGAAGGTGGTGCATTTACAAAAAAGCTTGTGTTGGCTACTCCAATGAAAGGCTGTGCACTGTACAAGGTCAAAAAATAATCAAAAGAATTGTACTTCCTGACATGATTCTAGCATTTCTTTCCTTGGCCACAAAATAACCTTTGACACATTTGACCACAACACCCTTGCTTTGTCTTTTAGACTCCACCCTTTCAAAAATTCCACCAGCCAAGCTTCTATAGACAATGACTTACTTCACTGCAAACCAAACGAACTGCCAAAAAAATCTCACACTTCCAACTTCTTACATCCTTGGACGGAGCCGTGCAGTGGACGCAGAGCGAACTTTTCTTTTGCCTTTTACTAAAGAATACTGTGTGTTCGCCCGCCATAAGCGGCATACGCCTATTTTTGGAGAGTCCTTCCTTCAGGATTTGGTCCAAAAAAAAAACTTCTTGCATCCTTGGATTTTCTTGGTTGGTTCTTGATTGATTGTCGGTAATCTTTCCCACTTCCAACGGTAACTTCTCAATATCTTGCTGCAACTGGGCCATTGAGGTTTGCATCAAAATCATATTGTCCACAAACCACCTACTAAAGTTTCCAAATTGCCAAGTCTTAGATTCACGATTTGAGGAGATGTGGAGAAGGATGCTTCCGCATCATGAGTTTGAAGGAAAATCTTGGAGACTACAAATCTTGGAGAATTCTTGAAGGAAAATCTTATTCATTAATTGTCCATACATGGGAGAAGACTACTATTTATAGAATTTTAATACAAGTGGGGGTAAAAAGGGAATTAACCTAGATCTATTACCTAATTACCCAATTTAACCCCATTCTACTTACATCATTGTACCTTGGAGCATTAGTCTCTTTTCATTCTCTTTATGAAAAATGTTGTATCCTTTTCAAAGAAAGGAAAAAAAAGAAAAAAAACAGAGTATATTGCTGACGTGTTAATTGAAAGACTTCTAAAGTTGCAGTTCAACAAACTGATCAGTAAGTTAGTCAAGTTAGTTATAGACGATATCTTCAAAGCAACATGAGGGGAATGTTGACAATTTCGTTTCTTTAAAATTGTATTTTCTTGTATGGGTTGTATTATATTTGCTAGTAGAACCTTTTTTTCTTCCTATTTAAGTAACCTAATTTCTCATTGTGAATAAGACCGAAATTATTATTCAGAGTAAAGTTTTTGATCTCTGTTTTAAGGTTTTGTTGATTTTTGGTGCATGAAATTATATTTTTTGTGACTTGTTTCCGTTTCAAAAAAAAAAAAAAAATTATCTTTTTTGTGGTGGTGGTGATTCATTGTTGTCAATTTTCTTCTTCTTCTTGTTTTTTTTTTGTTTTGCTTTCTTTCTTTCTTTTAGTCCAACGAGATATTTTTCTTAATGGATCTATTCTAATTTACAGGTCATGGTGGTTTTGGACACGTGGTATTATGCAAGAACAAATTAGATGGTCGACATTATGCTGTGAAAAAAATTCGCCTCAAGGACAAAATATTGCCCGTCGATGACCGCATCTTAAGGTGA

mRNA sequence

CTGCAGCATGAAGTCCATATACCCAGACGAGATTCTATTCTCACATACGATGTTGCTGAAGGTGATGATCATGGATCTGAAAGCGAGCATTCAGAGTGGTCTTTTGCTTCCTTGAGTAATGAACAAGAATCCCAAACTACTGATAGGGATATAATGATGGTTCATCTGCTCCATCTTGCATGTGCCCCTAAAGGACCGTTGGCTGATGCTCTGCCAAAATTGGCATCTGAACTTTACAATTTGGGGGTCCTGTCAAAAGTGGCTCTGGATTTAGCTTCTAAACCACCTTCCATGTTTCATAAAAAATTTGAGACTGCTTTCCAGGAACAGATGAATGCTACTAGTTTCTCCCAGTTTTGGAATCCTTCTGATTTTGGTGGCCCAACATCTTCTCAACTGAGTTCTCGGTATCTTAACGATTTTGAGGAGCTAAAGCCCCTTGGTCATGGTGGTTTTGGACACGTGGTATTATGCAAGAACAAATTAGATGGTCGACATTATGCTGTGAAAAAAATTCGCCTCAAGGACAAAATATTGCCCGTCGATGACCGCATCTTAAGGTGA

Coding sequence (CDS)

CTGCAGCATGAAGTCCATATACCCAGACGAGATTCTATTCTCACATACGATGTTGCTGAAGGTGATGATCATGGATCTGAAAGCGAGCATTCAGAGTGGTCTTTTGCTTCCTTGAGTAATGAACAAGAATCCCAAACTACTGATAGGGATATAATGATGGTTCATCTGCTCCATCTTGCATGTGCCCCTAAAGGACCGTTGGCTGATGCTCTGCCAAAATTGGCATCTGAACTTTACAATTTGGGGGTCCTGTCAAAAGTGGCTCTGGATTTAGCTTCTAAACCACCTTCCATGTTTCATAAAAAATTTGAGACTGCTTTCCAGGAACAGATGAATGCTACTAGTTTCTCCCAGTTTTGGAATCCTTCTGATTTTGGTGGCCCAACATCTTCTCAACTGAGTTCTCGGTATCTTAACGATTTTGAGGAGCTAAAGCCCCTTGGTCATGGTGGTTTTGGACACGTGGTATTATGCAAGAACAAATTAGATGGTCGACATTATGCTGTGAAAAAAATTCGCCTCAAGGACAAAATATTGCCCGTCGATGACCGCATCTTAAGGTGA

Protein sequence

LQHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLACAPKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWNPSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPVDDRILR

Homology

[Close]

BLAST of HG10003825 vs. NCBI nr
Match: XP_038885260.1 (eIF-2-alpha kinase GCN2 isoform X4 [Benincasa hispida])

HSP 1 Score: 343.6 bits (880), Expect = 1.1e-90
Identity = 171/186 (91.94%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 2   QHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLAC 61
           QHEV IP +DSILT +VAEGDDHGSESE SEWSFASL+NEQESQT DRDIMMVHLLHLAC
Sbjct: 159 QHEVLIPGQDSILTSNVAEGDDHGSESEPSEWSFASLNNEQESQTIDRDIMMVHLLHLAC 218

Query: 62  APKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN 121
           APKGPLADALPKLASELYNLGVLSKVALDLASKP S FHKKF+TAFQEQMNATSFSQFWN
Sbjct: 219 APKGPLADALPKLASELYNLGVLSKVALDLASKPSSTFHKKFKTAFQEQMNATSFSQFWN 278

Query: 122 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 181
           PSDFGG  SSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILP+
Sbjct: 279 PSDFGGSASSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPI 338

Query: 182 DDRILR 188
           +DRILR
Sbjct: 339 NDRILR 344

[Close]

BLAST of HG10003825 vs. NCBI nr
Match: XP_038885259.1 (eIF-2-alpha kinase GCN2 isoform X3 [Benincasa hispida])

HSP 1 Score: 343.6 bits (880), Expect = 1.1e-90
Identity = 171/186 (91.94%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 2   QHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLAC 61
           QHEV IP +DSILT +VAEGDDHGSESE SEWSFASL+NEQESQT DRDIMMVHLLHLAC
Sbjct: 199 QHEVLIPGQDSILTSNVAEGDDHGSESEPSEWSFASLNNEQESQTIDRDIMMVHLLHLAC 258

Query: 62  APKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN 121
           APKGPLADALPKLASELYNLGVLSKVALDLASKP S FHKKF+TAFQEQMNATSFSQFWN
Sbjct: 259 APKGPLADALPKLASELYNLGVLSKVALDLASKPSSTFHKKFKTAFQEQMNATSFSQFWN 318

Query: 122 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 181
           PSDFGG  SSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILP+
Sbjct: 319 PSDFGGSASSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPI 378

Query: 182 DDRILR 188
           +DRILR
Sbjct: 379 NDRILR 384

[Close]

BLAST of HG10003825 vs. NCBI nr
Match: XP_038885257.1 (eIF-2-alpha kinase GCN2 isoform X1 [Benincasa hispida])

HSP 1 Score: 343.6 bits (880), Expect = 1.1e-90
Identity = 171/186 (91.94%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 2   QHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLAC 61
           QHEV IP +DSILT +VAEGDDHGSESE SEWSFASL+NEQESQT DRDIMMVHLLHLAC
Sbjct: 292 QHEVLIPGQDSILTSNVAEGDDHGSESEPSEWSFASLNNEQESQTIDRDIMMVHLLHLAC 351

Query: 62  APKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN 121
           APKGPLADALPKLASELYNLGVLSKVALDLASKP S FHKKF+TAFQEQMNATSFSQFWN
Sbjct: 352 APKGPLADALPKLASELYNLGVLSKVALDLASKPSSTFHKKFKTAFQEQMNATSFSQFWN 411

Query: 122 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 181
           PSDFGG  SSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILP+
Sbjct: 412 PSDFGGSASSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPI 471

Query: 182 DDRILR 188
           +DRILR
Sbjct: 472 NDRILR 477

[Close]

BLAST of HG10003825 vs. NCBI nr
Match: XP_038885258.1 (eIF-2-alpha kinase GCN2 isoform X2 [Benincasa hispida])

HSP 1 Score: 343.6 bits (880), Expect = 1.1e-90
Identity = 171/186 (91.94%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 2   QHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLAC 61
           QHEV IP +DSILT +VAEGDDHGSESE SEWSFASL+NEQESQT DRDIMMVHLLHLAC
Sbjct: 292 QHEVLIPGQDSILTSNVAEGDDHGSESEPSEWSFASLNNEQESQTIDRDIMMVHLLHLAC 351

Query: 62  APKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN 121
           APKGPLADALPKLASELYNLGVLSKVALDLASKP S FHKKF+TAFQEQMNATSFSQFWN
Sbjct: 352 APKGPLADALPKLASELYNLGVLSKVALDLASKPSSTFHKKFKTAFQEQMNATSFSQFWN 411

Query: 122 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 181
           PSDFGG  SSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILP+
Sbjct: 412 PSDFGGSASSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPI 471

Query: 182 DDRILR 188
           +DRILR
Sbjct: 472 NDRILR 477

[Close]

BLAST of HG10003825 vs. NCBI nr
Match: XP_022150410.1 (eIF-2-alpha kinase GCN2 isoform X2 [Momordica charantia])

HSP 1 Score: 342.0 bits (876), Expect = 3.1e-90
Identity = 170/186 (91.40%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 2   QHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLAC 61
           QH+V +P +DSILT  VAE DDHGSESE SEWSFASLSN+QESQTT+RDIMMVHLLHLAC
Sbjct: 152 QHDVFMPAQDSILTDHVAEDDDHGSESEPSEWSFASLSNDQESQTTNRDIMMVHLLHLAC 211

Query: 62  APKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN 121
           APKGPLADALPKLASELYNLGVLS+VALDL SKP SMFHKKFETAFQEQMNATSFSQFWN
Sbjct: 212 APKGPLADALPKLASELYNLGVLSEVALDLTSKPSSMFHKKFETAFQEQMNATSFSQFWN 271

Query: 122 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 181
           PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGR YAVKKIRLKDKILPV
Sbjct: 272 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPV 331

Query: 182 DDRILR 188
           +DRILR
Sbjct: 332 NDRILR 337

[Close]

BLAST of HG10003825 vs. ExPASy Swiss-Prot
Match: Q9LX30 (eIF-2-alpha kinase GCN2 OS=Arabidopsis thaliana OX=3702 GN=GCN2 PE=2 SV=2)

HSP 1 Score: 202.6 bits (514), Expect = 3.9e-51
Identity = 107/181 (59.12%), Postives = 131/181 (72.38%), Query Frame = 0


Query: 9   RRDSILTYDVAEGDDHGSESEH-SEWSFASLSNEQESQTTDRDIMMVHLLHLACAPKGPL 68
           +++S L  D AE D   SESE    WS  SL+ +Q  Q + +D++MVHLL +AC  +GPL
Sbjct: 291 KKESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPL 350

Query: 69  ADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN-PSDFG 128
           ADALP++  EL+ LG+LS+  LDLASK    F++ FE AF + M +TS  QFW  PSD  
Sbjct: 351 ADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSC 410

Query: 129 GPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPVDDRIL 188
            P +S  SSRYLNDFEELKPLG GGFGHVVLCKNKLDGR YAVKKIRLKDK +PV+ RI+
Sbjct: 411 EPNASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIV 470

[Close]

BLAST of HG10003825 vs. ExPASy Swiss-Prot
Match: Q75JN1 (Probable serine/threonine-protein kinase ifkC OS=Dictyostelium discoideum OX=44689 GN=ifkC PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.3e-11
Identity = 61/199 (30.65%), Postives = 91/199 (45.73%), Query Frame = 0


Query: 43  ESQTTDR-DIMMVHLLHLACAPKGPLA-DALPKLASELYNLGVLSKVALDLA----SKPP 102
           +++T D+   M++HLL L C     +  D +  L  +L  +G+ ++  L L     S   
Sbjct: 342 DNKTIDKQQTMILHLLRLLCQNDSNITNDHIKTLGEQLVQMGIFNRNHLSLLNMHNSNDN 401

Query: 103 SMFHKKFETAFQEQMNATSFS------------------QFW--------------NPSD 162
            ++   F+  F +Q N  S S                  +FW              +PS 
Sbjct: 402 QIYQHIFQDYFLKQFNDISLSDLKNNVDSAANNSGHFLNKFWDTLNKTNENSGLKKSPST 461

Query: 163 F-----------GGPTSSQL-----SSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYA 188
           F           GG +S +       SRY +DFEE++ LG GGFG VV  +NKLDGR+YA
Sbjct: 462 FEYSGEGGGGGVGGGSSQKTINPHQQSRYHSDFEEIQLLGRGGFGQVVKVRNKLDGRYYA 521

[Close]

BLAST of HG10003825 vs. ExPASy Swiss-Prot
Match: Q9BQI3 (Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Homo sapiens OX=9606 GN=EIF2AK1 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 29/54 (53.70%), Postives = 39/54 (72.22%), Query Frame = 0


Query: 134 SSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPVDDRILR 188
           +SRYLN+FEEL  LG GG+G V   +NKLDG++YA+KKI +K     V  ++LR
Sbjct: 160 TSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGATKTVCMKVLR 213

[Close]

BLAST of HG10003825 vs. ExPASy Swiss-Prot
Match: Q9Z2R9 (Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Mus musculus OX=10090 GN=Eif2ak1 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 27/42 (64.29%), Postives = 34/42 (80.95%), Query Frame = 0


Query: 134 SSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLK 176
           +SRYLN+FEEL  LG GG+G V   +NKLDG+HYA+KKI +K
Sbjct: 160 TSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIK 201

[Close]

BLAST of HG10003825 vs. ExPASy Swiss-Prot
Match: Q63185 (Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Rattus norvegicus OX=10116 GN=Eif2ak1 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 27/42 (64.29%), Postives = 34/42 (80.95%), Query Frame = 0


Query: 134 SSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLK 176
           +SRYLN+FEEL  LG GG+G V   +NKLDG+HYA+KKI +K
Sbjct: 160 TSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIK 201

[Close]

BLAST of HG10003825 vs. ExPASy TrEMBL
Match: A0A6J1D9Z7 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111018572 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.5e-90
Identity = 170/186 (91.40%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 2   QHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLAC 61
           QH+V +P +DSILT  VAE DDHGSESE SEWSFASLSN+QESQTT+RDIMMVHLLHLAC
Sbjct: 282 QHDVFMPAQDSILTDHVAEDDDHGSESEPSEWSFASLSNDQESQTTNRDIMMVHLLHLAC 341

Query: 62  APKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN 121
           APKGPLADALPKLASELYNLGVLS+VALDL SKP SMFHKKFETAFQEQMNATSFSQFWN
Sbjct: 342 APKGPLADALPKLASELYNLGVLSEVALDLTSKPSSMFHKKFETAFQEQMNATSFSQFWN 401

Query: 122 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 181
           PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGR YAVKKIRLKDKILPV
Sbjct: 402 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPV 461

Query: 182 DDRILR 188
           +DRILR
Sbjct: 462 NDRILR 467

[Close]

BLAST of HG10003825 vs. ExPASy TrEMBL
Match: A0A6J1D8E1 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111018572 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.5e-90
Identity = 170/186 (91.40%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 2   QHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLAC 61
           QH+V +P +DSILT  VAE DDHGSESE SEWSFASLSN+QESQTT+RDIMMVHLLHLAC
Sbjct: 152 QHDVFMPAQDSILTDHVAEDDDHGSESEPSEWSFASLSNDQESQTTNRDIMMVHLLHLAC 211

Query: 62  APKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN 121
           APKGPLADALPKLASELYNLGVLS+VALDL SKP SMFHKKFETAFQEQMNATSFSQFWN
Sbjct: 212 APKGPLADALPKLASELYNLGVLSEVALDLTSKPSSMFHKKFETAFQEQMNATSFSQFWN 271

Query: 122 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 181
           PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGR YAVKKIRLKDKILPV
Sbjct: 272 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPV 331

Query: 182 DDRILR 188
           +DRILR
Sbjct: 332 NDRILR 337

[Close]

BLAST of HG10003825 vs. ExPASy TrEMBL
Match: A0A5A7SPB6 (eIF-2-alpha kinase GCN2 isoform X5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold121G00520 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 6.4e-89
Identity = 170/187 (90.91%), Postives = 172/187 (91.98%), Query Frame = 0


Query: 1   LQHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLA 60
           LQHEV I   DSILT DVAEG DHGSESE SEWSFAS SNEQESQTTDRDIMMVHLLHLA
Sbjct: 14  LQHEVLITGLDSILTSDVAEGGDHGSESEPSEWSFASFSNEQESQTTDRDIMMVHLLHLA 73

Query: 61  CAPKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFW 120
           CAPKGPLADALPKLASELYNLG+LSK ALDLASKP S FHKKFETAFQEQMNATSFSQFW
Sbjct: 74  CAPKGPLADALPKLASELYNLGILSKAALDLASKPSSTFHKKFETAFQEQMNATSFSQFW 133

Query: 121 NPSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILP 180
             SDFGG  SSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILP
Sbjct: 134 TASDFGGSASSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILP 193

Query: 181 VDDRILR 188
           V+DRILR
Sbjct: 194 VNDRILR 200

[Close]

BLAST of HG10003825 vs. ExPASy TrEMBL
Match: A0A1S3BE48 (eIF-2-alpha kinase GCN2 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103488674 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.9e-88
Identity = 169/186 (90.86%), Postives = 171/186 (91.94%), Query Frame = 0


Query: 2   QHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLAC 61
           QHEV I   DSILT DVAEG DHGSESE SEWSFAS SNEQESQTTDRDIMMVHLLHLAC
Sbjct: 291 QHEVLITGLDSILTSDVAEGGDHGSESEPSEWSFASFSNEQESQTTDRDIMMVHLLHLAC 350

Query: 62  APKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN 121
           APKGPLADALPKLASELYNLG+LSK ALDLASKP S FHKKFETAFQEQMNATSFSQFW 
Sbjct: 351 APKGPLADALPKLASELYNLGILSKAALDLASKPSSTFHKKFETAFQEQMNATSFSQFWT 410

Query: 122 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 181
            SDFGG  SSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV
Sbjct: 411 ASDFGGSASSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 470

Query: 182 DDRILR 188
           +DRILR
Sbjct: 471 NDRILR 476

[Close]

BLAST of HG10003825 vs. ExPASy TrEMBL
Match: A0A1S3BDF8 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103488674 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.9e-88
Identity = 169/186 (90.86%), Postives = 171/186 (91.94%), Query Frame = 0


Query: 2   QHEVHIPRRDSILTYDVAEGDDHGSESEHSEWSFASLSNEQESQTTDRDIMMVHLLHLAC 61
           QHEV I   DSILT DVAEG DHGSESE SEWSFAS SNEQESQTTDRDIMMVHLLHLAC
Sbjct: 158 QHEVLITGLDSILTSDVAEGGDHGSESEPSEWSFASFSNEQESQTTDRDIMMVHLLHLAC 217

Query: 62  APKGPLADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN 121
           APKGPLADALPKLASELYNLG+LSK ALDLASKP S FHKKFETAFQEQMNATSFSQFW 
Sbjct: 218 APKGPLADALPKLASELYNLGILSKAALDLASKPSSTFHKKFETAFQEQMNATSFSQFWT 277

Query: 122 PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 181
            SDFGG  SSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV
Sbjct: 278 ASDFGGSASSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPV 337

Query: 182 DDRILR 188
           +DRILR
Sbjct: 338 NDRILR 343

[Close]

BLAST of HG10003825 vs. TAIR 10
Match: AT3G59410.1 (protein kinase family protein )

HSP 1 Score: 202.6 bits (514), Expect = 2.8e-52
Identity = 107/181 (59.12%), Postives = 131/181 (72.38%), Query Frame = 0


Query: 9   RRDSILTYDVAEGDDHGSESEH-SEWSFASLSNEQESQTTDRDIMMVHLLHLACAPKGPL 68
           +++S L  D AE D   SESE    WS  SL+ +Q  Q + +D++MVHLL +AC  +GPL
Sbjct: 291 KKESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPL 350

Query: 69  ADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN-PSDFG 128
           ADALP++  EL+ LG+LS+  LDLASK    F++ FE AF + M +TS  QFW  PSD  
Sbjct: 351 ADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSC 410

Query: 129 GPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPVDDRIL 188
            P +S  SSRYLNDFEELKPLG GGFGHVVLCKNKLDGR YAVKKIRLKDK +PV+ RI+
Sbjct: 411 EPNASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIV 470

[Close]

BLAST of HG10003825 vs. TAIR 10
Match: AT3G59410.2 (protein kinase family protein )

HSP 1 Score: 202.6 bits (514), Expect = 2.8e-52
Identity = 107/181 (59.12%), Postives = 131/181 (72.38%), Query Frame = 0


Query: 9   RRDSILTYDVAEGDDHGSESEH-SEWSFASLSNEQESQTTDRDIMMVHLLHLACAPKGPL 68
           +++S L  D AE D   SESE    WS  SL+ +Q  Q + +D++MVHLL +AC  +GPL
Sbjct: 315 KKESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPL 374

Query: 69  ADALPKLASELYNLGVLSKVALDLASKPPSMFHKKFETAFQEQMNATSFSQFWN-PSDFG 128
           ADALP++  EL+ LG+LS+  LDLASK    F++ FE AF + M +TS  QFW  PSD  
Sbjct: 375 ADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSC 434

Query: 129 GPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKIRLKDKILPVDDRIL 188
            P +S  SSRYLNDFEELKPLG GGFGHVVLCKNKLDGR YAVKKIRLKDK +PV+ RI+
Sbjct: 435 EPNASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIV 494

[Close]

BLAST of HG10003825 vs. TAIR 10
Match: AT4G09012.1 (Mitochondrial ribosomal protein L27 )

HSP 1 Score: 42.4 bits (98), Expect = 4.8e-04
Identity = 21/38 (55.26%), Postives = 25/38 (65.79%), Query Frame = 0


Query: 135 SRYLNDFEELKPLGHGGFGHVVLCKNKLDGRHYAVKKI 173
           SRY +DF E   LG GG+G VV C  K+D + YAVK I
Sbjct: 15  SRYHDDFIEEGSLGRGGYGKVVQCVFKMDQKVYAVKGI 52

The following BLAST results are available for this feature:

BLAST of HG10003825 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885260.1	1.1e-90	91.94	eIF-2-alpha kinase GCN2 isoform X4 [Benincasa hispida]	[more]
XP_038885259.1	1.1e-90	91.94	eIF-2-alpha kinase GCN2 isoform X3 [Benincasa hispida]	[more]
XP_038885257.1	1.1e-90	91.94	eIF-2-alpha kinase GCN2 isoform X1 [Benincasa hispida]	[more]
XP_038885258.1	1.1e-90	91.94	eIF-2-alpha kinase GCN2 isoform X2 [Benincasa hispida]	[more]
XP_022150410.1	3.1e-90	91.40	eIF-2-alpha kinase GCN2 isoform X2 [Momordica charantia]	[more]

BLAST of HG10003825 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LX30	3.9e-51	59.12	eIF-2-alpha kinase GCN2 OS=Arabidopsis thaliana OX=3702 GN=GCN2 PE=2 SV=2	[more]
Q75JN1	2.3e-11	30.65	Probable serine/threonine-protein kinase ifkC OS=Dictyostelium discoideum OX=446...	[more]
Q9BQI3	1.1e-08	53.70	Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Homo sapiens OX=960...	[more]
Q9Z2R9	1.1e-08	64.29	Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Mus musculus OX=100...	[more]
Q63185	1.1e-08	64.29	Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Rattus norvegicus O...	[more]

BLAST of HG10003825 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D9Z7	1.5e-90	91.40	Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1D8E1	1.5e-90	91.40	Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L...	[more]
A0A5A7SPB6	6.4e-89	90.91	eIF-2-alpha kinase GCN2 isoform X5 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BE48	1.9e-88	90.86	eIF-2-alpha kinase GCN2 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103488674 PE=4 ...	[more]
A0A1S3BDF8	1.9e-88	90.86	Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348...	[more]

BLAST of HG10003825 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G59410.1	2.8e-52	59.12	protein kinase family protein	[more]
AT3G59410.2	2.8e-52	59.12	protein kinase family protein	[more]
AT4G09012.1	4.8e-04	55.26	Mitochondrial ribosomal protein L27	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 182..187
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 129..187 e-value: 4.8E-17 score: 64.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 20..41
None	No IPR available	PANTHER	PTHR11476	HISTIDYL-TRNA SYNTHETASE	coord: 18..185
None	No IPR available	PANTHER	PTHR11476:SF10	EIF-2-ALPHA KINASE GCN2	coord: 18..185
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 147..171
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 141..187 score: 9.432338
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 118..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10003825.1	HG10003825.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018105	peptidyl-serine phosphorylation
biological_process	GO:0006521	regulation of cellular amino acid metabolic process
biological_process	GO:0010998	regulation of translational initiation by eIF2 alpha phosphorylation
biological_process	GO:0009635	response to herbicide
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004694	eukaryotic translation initiation factor 2alpha kinase activity
molecular_function	GO:0106310	protein serine kinase activity
molecular_function	GO:0004712	protein serine/threonine/tyrosine kinase activity
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0004672	protein kinase activity