HG10003788 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003788
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	rRNA N-glycosidase
Location	Chr08: 8865454 .. 8866362 (-)
RNA-Seq Expression	HG10003788
Synteny	HG10003788

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTGATTCAAACATTTCCCTTAAATTGCGGTGCTGCTCGTGTTGCTCGTGTCGCCAATCATGTCGATCGTCGTCCGGCCAATCCGAATCTCAGCTTCTCTCTAATTGGTGCCACTGCTTCTACTTACAAACAATTCATAGCAAATCTGCGTAAGACACTTACACTAAACTCCCCCACAGTCTACGGTATACCCGTCTTAGCATCCACAGCCAAAGGCCTAAACCGCTTCATATTGATCAATCTCACCAATTACCAAGCTGAATCCATCACTATTGCAATTGACGTAGTTAACGTTTACATAGTTGCATACCAAGCCCGAAACAAAGCCTATTTTCTCAAGAACGTTTCCAAAGAAACACATGATGTTTTATTTAAAGATATGAAACATGAAACACTTCCCTACAAAGGGAATTACGATGATCTTGAGAGATTTGCTGGAAATATTTCGAGAGAGAATATTGATCTTGGATTATCTGAATTAGGGAGTTCCATTGGCAACTTGTTTCATTATAAGGCTGGGAATTCTGTGCCAAGAGCATTGATTGTGATCATTCAAACGATTTCTGAAGCTGCAAGATTTAAGTATATTGAGTCACAGTTTGTTAGATCTAAGTTTAAACCGGACCCTGGGTTTATAAGCTTAGAAAATCGTTGGTCCGATCTGTGCGAACAGATACAGAAGGCGCAAAAGCTAGGAGGGAAATTTAAGAGTCCAGTTCCGGTTCGAAGTGTTAGCAATAAACCGATAATGGTAAGTGATGTTAATTCAGCTGTGGTTAAAGGGATTGCGTTATTGCTTCATGATAAGGCTGCAGAAGCAAAAAATGGAAACCTTAAACTGATGATGGGAGAGGTTAATAATCAATGGGAAGCTCCACGAGGTGATTTTGGAATTGATTTCTGA

mRNA sequence

ATGGAGTTGATTCAAACATTTCCCTTAAATTGCGGTGCTGCTCGTGTTGCTCGTGTCGCCAATCATGTCGATCGTCGTCCGGCCAATCCGAATCTCAGCTTCTCTCTAATTGGTGCCACTGCTTCTACTTACAAACAATTCATAGCAAATCTGCGTAAGACACTTACACTAAACTCCCCCACAGTCTACGGTATACCCGTCTTAGCATCCACAGCCAAAGGCCTAAACCGCTTCATATTGATCAATCTCACCAATTACCAAGCTGAATCCATCACTATTGCAATTGACGTAGTTAACGTTTACATAGTTGCATACCAAGCCCGAAACAAAGCCTATTTTCTCAAGAACGTTTCCAAAGAAACACATGATGTTTTATTTAAAGATATGAAACATGAAACACTTCCCTACAAAGGGAATTACGATGATCTTGAGAGATTTGCTGGAAATATTTCGAGAGAGAATATTGATCTTGGATTATCTGAATTAGGGAGTTCCATTGGCAACTTGTTTCATTATAAGGCTGGGAATTCTGTGCCAAGAGCATTGATTGTGATCATTCAAACGATTTCTGAAGCTGCAAGATTTAAGTATATTGAGTCACAGTTTGTTAGATCTAAGTTTAAACCGGACCCTGGGTTTATAAGCTTAGAAAATCGTTGGTCCGATCTGTGCGAACAGATACAGAAGGCGCAAAAGCTAGGAGGGAAATTTAAGAGTCCAGTTCCGGTTCGAAGTGTTAGCAATAAACCGATAATGGTAAGTGATGTTAATTCAGCTGTGGTTAAAGGGATTGCGTTATTGCTTCATGATAAGGCTGCAGAAGCAAAAAATGGAAACCTTAAACTGATGATGGGAGAGGTTAATAATCAATGGGAAGCTCCACGAGGTGATTTTGGAATTGATTTCTGA

Coding sequence (CDS)

ATGGAGTTGATTCAAACATTTCCCTTAAATTGCGGTGCTGCTCGTGTTGCTCGTGTCGCCAATCATGTCGATCGTCGTCCGGCCAATCCGAATCTCAGCTTCTCTCTAATTGGTGCCACTGCTTCTACTTACAAACAATTCATAGCAAATCTGCGTAAGACACTTACACTAAACTCCCCCACAGTCTACGGTATACCCGTCTTAGCATCCACAGCCAAAGGCCTAAACCGCTTCATATTGATCAATCTCACCAATTACCAAGCTGAATCCATCACTATTGCAATTGACGTAGTTAACGTTTACATAGTTGCATACCAAGCCCGAAACAAAGCCTATTTTCTCAAGAACGTTTCCAAAGAAACACATGATGTTTTATTTAAAGATATGAAACATGAAACACTTCCCTACAAAGGGAATTACGATGATCTTGAGAGATTTGCTGGAAATATTTCGAGAGAGAATATTGATCTTGGATTATCTGAATTAGGGAGTTCCATTGGCAACTTGTTTCATTATAAGGCTGGGAATTCTGTGCCAAGAGCATTGATTGTGATCATTCAAACGATTTCTGAAGCTGCAAGATTTAAGTATATTGAGTCACAGTTTGTTAGATCTAAGTTTAAACCGGACCCTGGGTTTATAAGCTTAGAAAATCGTTGGTCCGATCTGTGCGAACAGATACAGAAGGCGCAAAAGCTAGGAGGGAAATTTAAGAGTCCAGTTCCGGTTCGAAGTGTTAGCAATAAACCGATAATGGTAAGTGATGTTAATTCAGCTGTGGTTAAAGGGATTGCGTTATTGCTTCATGATAAGGCTGCAGAAGCAAAAAATGGAAACCTTAAACTGATGATGGGAGAGGTTAATAATCAATGGGAAGCTCCACGAGGTGATTTTGGAATTGATTTCTGA

Protein sequence

MELIQTFPLNCGAARVARVANHVDRRPANPNLSFSLIGATASTYKQFIANLRKTLTLNSPTVYGIPVLASTAKGLNRFILINLTNYQAESITIAIDVVNVYIVAYQARNKAYFLKNVSKETHDVLFKDMKHETLPYKGNYDDLERFAGNISRENIDLGLSELGSSIGNLFHYKAGNSVPRALIVIIQTISEAARFKYIESQFVRSKFKPDPGFISLENRWSDLCEQIQKAQKLGGKFKSPVPVRSVSNKPIMVSDVNSAVVKGIALLLHDKAAEAKNGNLKLMMGEVNNQWEAPRGDFGIDF

Homology

[Close]

BLAST of HG10003788 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 397.1 bits (1019), Expect = 1.3e-106
Identity = 216/305 (70.82%), Postives = 242/305 (79.34%), Query Frame = 0


Query: 2   ELIQT-FPLNCGAARVARVANHVDRRPANPNLSFSLIGATASTYKQFIANLRKTLTLNSP 61
           +LIQ  FPLNCG    A  A    R  AN NLSFSL+GATA TYKQFI +LRK LT+NS 
Sbjct: 9   DLIQAPFPLNCG----AHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSH 68

Query: 62  TVYGIPVLASTAKGLNRFILINLTNYQAESITIAIDVVNVYIVAYQARNKAYFLKNVSKE 121
            VY IPVL +TAKGL RFIL+NLTNY+ ESIT+AID V+VYIVAYQA NKAYFLK+VSKE
Sbjct: 69  KVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKE 128

Query: 122 THDVLFKDMKHETLPYKGNYDDLERFAGNISRENIDLGLSELGSSIGNLFHYKAGNSVPR 181
            HDVLFK  KHETL YKGNYDDLER AG ISRENI+LG SEL SSIGN+FHYKAG SVPR
Sbjct: 129 AHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPR 188

Query: 182 ALIVIIQTISEAARFKYIES---QFVRSKFKPDPGFISLENRWSDLCEQIQKAQKLGGKF 241
           + IV+IQ +SEA+RFKYIE+   + V SKFKPDPG++SLENRWS+L EQ+QKAQK GGKF
Sbjct: 189 SFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKF 248

Query: 242 KSPVPVRSVSNKPIMVSDVNSAVVKGIALLLHDKAAEAKNGNLKLMMGEVNNQWEAPRGD 301
           + PV VRSVSNKP++VSDVNS VVKGIALLL +K     NG  KLM  E    WEA RG 
Sbjct: 249 EHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNK--PGVNGVSKLM--EEFIHWEAARGH 305

Query: 302 FGIDF 303
           FGIDF
Sbjct: 309 FGIDF 305

[Close]

BLAST of HG10003788 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 396.7 bits (1018), Expect = 1.7e-106
Identity = 215/305 (70.49%), Postives = 244/305 (80.00%), Query Frame = 0


Query: 2   ELIQT-FPLNCGAARVARVANHVDRRPANPNLSFSLIGATASTYKQFIANLRKTLTLNSP 61
           +LIQ  FPLNCG    A VA    R  AN NLSFSL+GATA TYKQFI++LRK LT+NS 
Sbjct: 9   DLIQAPFPLNCG----AHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALTVNSH 68

Query: 62  TVYGIPVLASTAKGLNRFILINLTNYQAESITIAIDVVNVYIVAYQARNKAYFLKNVSKE 121
            VY IPVL +TAKGL RFIL+NLTNY+ ESIT+AID V+VYIVAYQA NKAYFLK+VSKE
Sbjct: 69  KVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKE 128

Query: 122 THDVLFKDMKHETLPYKGNYDDLERFAGNISRENIDLGLSELGSSIGNLFHYKAGNSVPR 181
            HDVLFK  KHETL YKGNYDDLER AG ISRENIDLG SEL SSIGN+FHY AG SVP+
Sbjct: 129 AHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGYSVPK 188

Query: 182 ALIVIIQTISEAARFKYIES---QFVRSKFKPDPGFISLENRWSDLCEQIQKAQKLGGKF 241
           + IV+IQ +SEA+RFKYIE+   + V SKFKPDPG++SLENRWS+L EQ+QKAQK GGKF
Sbjct: 189 SFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKF 248

Query: 242 KSPVPVRSVSNKPIMVSDVNSAVVKGIALLLHDKAAEAKNGNLKLMMGEVNNQWEAPRGD 301
           + PV VRSVSNKP++VSDVNS VVKG+ALLL +K     NG  KLM  E   +WEA RG 
Sbjct: 249 EHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNK--PGVNGVSKLM--EEFIKWEAARGH 305

Query: 302 FGIDF 303
           FGIDF
Sbjct: 309 FGIDF 305

[Close]

BLAST of HG10003788 vs. NCBI nr
Match: KAG7037232.1 (hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.5 bits (1007), Expect = 3.3e-105
Identity = 214/305 (70.16%), Postives = 242/305 (79.34%), Query Frame = 0


Query: 2   ELIQT-FPLNCGAARVARVANHVDRRPANPNLSFSLIGATASTYKQFIANLRKTLTLNSP 61
           +LIQ  FPLNCG    A  A    R  AN NLSFSL+GATA TYKQFI +LRK LT+NS 
Sbjct: 9   DLIQAPFPLNCG----AHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSH 68

Query: 62  TVYGIPVLASTAKGLNRFILINLTNYQAESITIAIDVVNVYIVAYQARNKAYFLKNVSKE 121
            VY IPVL +TAKGL RFIL+NLTNY+ ESIT+AID V+VYIVAYQA NKAYFLK+VSKE
Sbjct: 69  KVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKE 128

Query: 122 THDVLFKDMKHETLPYKGNYDDLERFAGNISRENIDLGLSELGSSIGNLFHYKAGNSVPR 181
            HDVLFK  KHETL YKGNYDDLER AG ISRENI+LG SEL SSIGN+FHY AG SVPR
Sbjct: 129 AHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNAGYSVPR 188

Query: 182 ALIVIIQTISEAARFKYIES---QFVRSKFKPDPGFISLENRWSDLCEQIQKAQKLGGKF 241
           + IV+IQ +SEA+RFKYIE+   + V SKFKPDPG++SLENRWS+L EQ+QKAQK GGKF
Sbjct: 189 SFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKF 248

Query: 242 KSPVPVRSVSNKPIMVSDVNSAVVKGIALLLHDKAAEAKNGNLKLMMGEVNNQWEAPRGD 301
           + PV VRSVSNKP++VSDVNS VVKGIALLL +K     NG  KLM  E   +WEA RG 
Sbjct: 249 EHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNK--PGVNGVSKLM--EEFIKWEAARGA 305

Query: 302 FGIDF 303
           FGI+F
Sbjct: 309 FGINF 305

[Close]

BLAST of HG10003788 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389.8 bits (1000), Expect = 2.1e-104
Identity = 214/305 (70.16%), Postives = 239/305 (78.36%), Query Frame = 0


Query: 2   ELIQT-FPLNCGAARVARVANHVDRRPANPNLSFSLIGATASTYKQFIANLRKTLTLNSP 61
           +LIQ  FPLNCG    A  A    R  AN NLSFSL+GATA TYKQFI +LR  LT+ S 
Sbjct: 9   DLIQAPFPLNCG----AHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRNALTVQSH 68

Query: 62  TVYGIPVLASTAKGLNRFILINLTNYQAESITIAIDVVNVYIVAYQARNKAYFLKNVSKE 121
            VYGIPVL +TAKGL RFIL+NLTNY+ ESIT+AID V+VYIVAYQA NKAYFLK+VSKE
Sbjct: 69  KVYGIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKE 128

Query: 122 THDVLFKDMKHETLPYKGNYDDLERFAGNISRENIDLGLSELGSSIGNLFHYKAGNSVPR 181
            HDVLFK  KHETL YKGNYDDLER AG ISRENI+LG SEL SSIGN+FHY AG SVPR
Sbjct: 129 AHDVLFKGSKHETLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNMFHYNAGYSVPR 188

Query: 182 ALIVIIQTISEAARFKYIES---QFVRSKFKPDPGFISLENRWSDLCEQIQKAQKLGGKF 241
           + IV+IQ +SEA+RFKYIE+   + V SKFKPDPG++SLENRWS+L EQ+QKAQK GGKF
Sbjct: 189 SFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKF 248

Query: 242 KSPVPVRSVSNKPIMVSDVNSAVVKGIALLLHDKAAEAKNGNLKLMMGEVNNQWEAPRGD 301
           + PV VRSVSNKPI V+DVNS VVKGIALLL +K     NG  KLM  E    WEA RG 
Sbjct: 249 ERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNK--PGVNGVSKLM--EQFIHWEAARGA 305

Query: 302 FGIDF 303
           FGIDF
Sbjct: 309 FGIDF 305

[Close]

BLAST of HG10003788 vs. NCBI nr
Match: KAG7037234.1 (hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 383.3 bits (983), Expect = 2.0e-102
Identity = 211/305 (69.18%), Postives = 236/305 (77.38%), Query Frame = 0


Query: 2   ELIQT-FPLNCGAARVARVANHVDRRPANPNLSFSLIGATASTYKQFIANLRKTLTLNSP 61
           +LIQ  FPLNCG    A  A  V R  AN N+SFSL+GATA TYKQFI +LRK LT+NS 
Sbjct: 9   DLIQAPFPLNCG----AHAAIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSH 68

Query: 62  TVYGIPVLASTAKGLNRFILINLTNYQAESITIAIDVVNVYIVAYQARNKAYFLKNVSKE 121
            VYGIPVL +TAKGL RFIL+NLTNY  ESIT+AID V+VYIVAYQA NKAYFLK+VSKE
Sbjct: 69  KVYGIPVLPATAKGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKE 128

Query: 122 THDVLFKDMKHETLPYKGNYDDLERFAGNISRENIDLGLSELGSSIGNLFHYKAGNSVPR 181
            H+VLFK  KHETL YKGNYDDLER AG ISREN+ LG SEL SSIGN+FHY AG SVPR
Sbjct: 129 AHNVLFKGSKHETLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPR 188

Query: 182 ALIVIIQTISEAARFKYIE---SQFVRSKFKPDPGFISLENRWSDLCEQIQKAQKLGGKF 241
           + IV+IQ +SEA+RFKYIE   S  V S FKPDPG++SLENRW++L EQ+QKAQK GGKF
Sbjct: 189 SFIVMIQMLSEASRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKF 248

Query: 242 KSPVPVRSVSNKPIMVSDVNSAVVKGIALLLHDKAAEAKNGNLKLMMGEVNNQWEAPRGD 301
           +  V VRSV NK I V+DVNS VVKGIALLL +K     NG  KLM  E    WEA RGD
Sbjct: 249 EHAVQVRSVGNKSIKVTDVNSPVVKGIALLLCNK--PGANGVSKLM--EEFIHWEAARGD 305

Query: 302 FGIDF 303
           FGIDF
Sbjct: 309 FGIDF 305

[Close]

BLAST of HG10003788 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 3.9e-77
Identity = 150/242 (61.98%), Postives = 183/242 (75.62%), Query Frame = 0


Query: 31  NLSFSLIGATASTYKQFIANLRKTLTLNSPTVYGIPVLASTAKGLNRFILINLTNYQAES 90
           +++FSLIGAT +TYK FI NLR  LT+ +P VY IPVL + A GL RF L+ LTNY  ES
Sbjct: 23  DINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAAAGLARFQLVTLTNYNGES 82

Query: 91  ITIAIDVVNVYIVAYQARNKAYFLKNVSKETHDVLFKDMKHETLPYKGNYDDLERFAGNI 150
           +T+A+DVVNVY+VAY+A N AYFL + S E ++VLF  + H  LPY GNYD LE  AG I
Sbjct: 83  VTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHVRLPYGGNYDGLETAAGRI 142

Query: 151 SRENIDLGLSELGSSIGNLFHYKAGNSVPRALIVIIQTISEAARFKYIE---SQFVRSKF 210
           SRENI+LG SE+ S+IGN+F +  G SVPRA IVIIQT+SEAARFKYIE   S+ V +KF
Sbjct: 143 SRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEAARFKYIEQRVSENVGTKF 202

Query: 211 KPDPGFISLENRWSDLCEQIQKAQKLGGKFKSPVPVRSVSNKPIMVSDVNSAVVKGIALL 270
           KPDP F+SL+N W  L EQIQ AQ  GG+F  PV +R+VSN P  V++VNS VVKGIALL
Sbjct: 203 KPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNTPTFVTNVNSPVVKGIALL 262

[Close]

BLAST of HG10003788 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 9.4e-55
Identity = 127/260 (48.85%), Postives = 173/260 (66.54%), Query Frame = 0


Query: 28  ANPNLSFSLIGATASTYKQFIANLRKTLTLNSPTVYGIPVLASTAKGLNRFILINLTNYQ 87
           A  +++F L  ATA TY +FI + R TL   S  VY IP+L ST     RFIL+NLT+Y 
Sbjct: 21  AKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTSYA 80

Query: 88  AESITIAIDVVNVYIVAYQARNKAYFLKNVSKETHDVLFKDMKHETLPYKGNYDDLERFA 147
            E+I++AIDV NVY+VAY+ R+ +YF K    E +++LFK  +  TLPY GNY++L+  A
Sbjct: 81  YETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQTAA 140

Query: 148 GNISRENIDLGLSELGSSIGNLFHYKAGNSVPRALIVIIQTISEAARFKYIE---SQFVR 207
             I RENIDLGL  L S+I  LF+Y A  S P AL+V+IQT +EAARFKYIE   +++V 
Sbjct: 141 HKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKYVA 200

Query: 208 SKFKPDPGFISLENRWSDLCEQIQKAQKLGGKFKSPVPVRSVSNKPIMVSDVNSAVVKG- 267
           + FKP+   ISLEN+WS L +QI  AQ  GGKF++PV +   + +   V++V+S VVKG 
Sbjct: 201 TNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVKGN 260

Query: 268 IALLLHDKAAEAKNGNLKLM 284
           I LLL+ +A+ A    +  M
Sbjct: 261 IKLLLNSRASTADENFITTM 277

[Close]

BLAST of HG10003788 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-54
Identity = 126/260 (48.46%), Postives = 173/260 (66.54%), Query Frame = 0


Query: 28  ANPNLSFSLIGATASTYKQFIANLRKTLTLNSPTVYGIPVLASTAKGLNRFILINLTNYQ 87
           A  +++F L  ATA TY +FI + R TL   S  VY IP+L ST     RFIL++LT+Y 
Sbjct: 21  AKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLDLTSYA 80

Query: 88  AESITIAIDVVNVYIVAYQARNKAYFLKNVSKETHDVLFKDMKHETLPYKGNYDDLERFA 147
            E+I++AIDV NVY+VAY+ R+ +YF K    E +++LFK  +  TLPY GNY++L+  A
Sbjct: 81  YETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQTAA 140

Query: 148 GNISRENIDLGLSELGSSIGNLFHYKAGNSVPRALIVIIQTISEAARFKYIE---SQFVR 207
             I RENIDLGL  L S+I  LF+Y A  S P AL+V+IQT +EAARFKYIE   +++V 
Sbjct: 141 HKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIERHVAKYVA 200

Query: 208 SKFKPDPGFISLENRWSDLCEQIQKAQKLGGKFKSPVPVRSVSNKPIMVSDVNSAVVKG- 267
           + FKP+   ISLEN+WS L +QI  AQ  GGKF++PV +   + +   V++V+S VVKG 
Sbjct: 201 TNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVKGN 260

Query: 268 IALLLHDKAAEAKNGNLKLM 284
           I LLL+ +A+ A    +  M
Sbjct: 261 IKLLLNSRASTADENFITTM 277

[Close]

BLAST of HG10003788 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 197.6 bits (501), Expect = 2.0e-49
Identity = 119/243 (48.97%), Postives = 155/243 (63.79%), Query Frame = 0


Query: 31  NLSFSLIGATASTYKQFIANLRKTLTLNSPTVYGIPVLASTAKGLNRFILINLTNYQAES 90
           N+ F L  AT+S+YK FI NLR+ L  +   VY IPVL ST     RFILI+L NY  +S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDG-KVYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 91  ITIAIDVVNVYIVAYQARNKAYFLKNVSKETHDVLFKDMKHETLPYKGNYDDLERFAGNI 150
           IT AIDV+NVYIVAY     +YF + V  +   +LFK  +  TLPY GNY++L+  A  +
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 151 SRENIDLGLSELGSSIGNLFHYKAGNSVPRALIVIIQTISEAARFKYIESQF---VRSKF 210
            RENI+LGL  L S+I  LFHY A  +   AL+V+IQT SEAARF+YIE Q    V +KF
Sbjct: 121 -RENIELGLPALDSAITTLFHYNA-EAAASALLVLIQTTSEAARFRYIELQIANNVGTKF 180

Query: 211 KPDPGFISLENRWSDLCEQIQKAQKLGGKFKSPVPVRSVSNKPIMVSDVNSAVV-KGIAL 270
           KP    ISLEN WS L +QIQ A+   G+F++PV +       + +++V S VV + I L
Sbjct: 181 KPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQL 240

[Close]

BLAST of HG10003788 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 191.0 bits (484), Expect = 1.9e-47
Identity = 119/245 (48.57%), Postives = 160/245 (65.31%), Query Frame = 0


Query: 31  NLSFSLIGATASTYKQFIANLRKTLTLNSPTVYGIPVLASTAKGLNRFILINLTNYQAES 90
           ++SF L GAT+S+Y  FI+NLRK L  N   +Y IP+L S+  G  R+ LI+LTNY  E+
Sbjct: 24  DVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSSLPGSQRYALIHLTNYADET 83

Query: 91  ITIAIDVVNVYIVAYQARNKAYFLKNVS-KETHDVLFKD-MKHETLPYKGNYDDLERFAG 150
           I++AIDV NVYI+ Y+A + +YF    S  E    +FKD M+  TLPY GNY+ L+  AG
Sbjct: 84  ISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLPYSGNYERLQTAAG 143

Query: 151 NISRENIDLGLSELGSSIGNLFHYKAGNSVPRALIVIIQTISEAARFKYIESQF---VRS 210
            I RENI LGL  L S+I  LF+Y A NS   AL+V+IQ+ SEAAR+K+IE Q    V  
Sbjct: 144 KI-RENIPLGLPALDSAITTLFYYNA-NSAASALMVLIQSTSEAARYKFIEQQIGKRVDK 203

Query: 211 KFKPDPGFISLENRWSDLCEQIQKAQKLGGKFKSPVPVRSVSNKPIMVSDVNSAVV-KGI 270
            F P    ISLEN WS L +QIQ A    G+F+SPV + +  N+ + +++V++ VV   I
Sbjct: 204 TFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVTITNVDAGVVTSNI 263

[Close]

BLAST of HG10003788 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 6.5e-107
Identity = 216/305 (70.82%), Postives = 242/305 (79.34%), Query Frame = 0


Query: 2   ELIQT-FPLNCGAARVARVANHVDRRPANPNLSFSLIGATASTYKQFIANLRKTLTLNSP 61
           +LIQ  FPLNCG    A  A    R  AN NLSFSL+GATA TYKQFI +LRK LT+NS 
Sbjct: 9   DLIQAPFPLNCG----AHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALTVNSH 68

Query: 62  TVYGIPVLASTAKGLNRFILINLTNYQAESITIAIDVVNVYIVAYQARNKAYFLKNVSKE 121
            VY IPVL +TAKGL RFIL+NLTNY+ ESIT+AID V+VYIVAYQA NKAYFLK+VSKE
Sbjct: 69  KVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKE 128

Query: 122 THDVLFKDMKHETLPYKGNYDDLERFAGNISRENIDLGLSELGSSIGNLFHYKAGNSVPR 181
            HDVLFK  KHETL YKGNYDDLER AG ISRENI+LG SEL SSIGN+FHYKAG SVPR
Sbjct: 129 AHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGYSVPR 188

Query: 182 ALIVIIQTISEAARFKYIES---QFVRSKFKPDPGFISLENRWSDLCEQIQKAQKLGGKF 241
           + IV+IQ +SEA+RFKYIE+   + V SKFKPDPG++SLENRWS+L EQ+QKAQK GGKF
Sbjct: 189 SFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKF 248

Query: 242 KSPVPVRSVSNKPIMVSDVNSAVVKGIALLLHDKAAEAKNGNLKLMMGEVNNQWEAPRGD 301
           + PV VRSVSNKP++VSDVNS VVKGIALLL +K     NG  KLM  E    WEA RG 
Sbjct: 249 EHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNK--PGVNGVSKLM--EEFIHWEAARGH 305

Query: 302 FGIDF 303
           FGIDF
Sbjct: 309 FGIDF 305

[Close]

BLAST of HG10003788 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 8.4e-107
Identity = 215/305 (70.49%), Postives = 244/305 (80.00%), Query Frame = 0


Query: 2   ELIQT-FPLNCGAARVARVANHVDRRPANPNLSFSLIGATASTYKQFIANLRKTLTLNSP 61
           +LIQ  FPLNCG    A VA    R  AN NLSFSL+GATA TYKQFI++LRK LT+NS 
Sbjct: 9   DLIQAPFPLNCG----AHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALTVNSH 68

Query: 62  TVYGIPVLASTAKGLNRFILINLTNYQAESITIAIDVVNVYIVAYQARNKAYFLKNVSKE 121
            VY IPVL +TAKGL RFIL+NLTNY+ ESIT+AID V+VYIVAYQA NKAYFLK+VSKE
Sbjct: 69  KVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKE 128

Query: 122 THDVLFKDMKHETLPYKGNYDDLERFAGNISRENIDLGLSELGSSIGNLFHYKAGNSVPR 181
            HDVLFK  KHETL YKGNYDDLER AG ISRENIDLG SEL SSIGN+FHY AG SVP+
Sbjct: 129 AHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGYSVPK 188

Query: 182 ALIVIIQTISEAARFKYIES---QFVRSKFKPDPGFISLENRWSDLCEQIQKAQKLGGKF 241
           + IV+IQ +SEA+RFKYIE+   + V SKFKPDPG++SLENRWS+L EQ+QKAQK GGKF
Sbjct: 189 SFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKF 248

Query: 242 KSPVPVRSVSNKPIMVSDVNSAVVKGIALLLHDKAAEAKNGNLKLMMGEVNNQWEAPRGD 301
           + PV VRSVSNKP++VSDVNS VVKG+ALLL +K     NG  KLM  E   +WEA RG 
Sbjct: 249 EHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNK--PGVNGVSKLM--EEFIKWEAARGH 305

Query: 302 FGIDF 303
           FGIDF
Sbjct: 309 FGIDF 305

[Close]

BLAST of HG10003788 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.9e-95
Identity = 187/277 (67.51%), Postives = 219/277 (79.06%), Query Frame = 0


Query: 29  NPNLSFSLIGATASTYKQFIANLRKTLTLNSPTVYGIPVLASTAKGLNRFILINLTNYQA 88
           N N+SFSL+GAT  TY+QFI NLR  LT+NS  VY IPVLA+TA    RFIL++LTNY+ 
Sbjct: 34  NSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKN 93

Query: 89  ESITIAIDVVNVYIVAYQARNKAYFLKNVSKETHDVLFKDMKHETLPYKGNYDDLERFAG 148
           E+IT+AIDVVNVYIVAYQA NKAYFLK+ SKE  DVLFK +K E LPYKGNYD LE  AG
Sbjct: 94  ETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAG 153

Query: 149 NISRENIDLGLSELGSSIGNLFHYKAGNSVPRALIVIIQTISEAARFKYIE---SQFVRS 208
            ISRE IDLG SELGS+IGN++HY AG SVPRA IV+IQTISEAARFKYIE   SQ V +
Sbjct: 154 KISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVET 213

Query: 209 KFKPDPGFISLENRWSDLCEQIQKAQKLGGKFKSPVPVRSVSNKPIMVSDVNSAVVKGIA 268
           KFKPDPGF+SLENRWSDL EQ+Q AQ   G+F  P+ VRSV+NKPI+V++V S VV+G+A
Sbjct: 214 KFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMA 273

Query: 269 LLLHDKAAEAKNGNLKLMMGEVNNQWEAPRGDFGIDF 303
           LLL+ K     NG+L  +M E N +WE  RG+FGIDF
Sbjct: 274 LLLYSK--PRGNGDLMELMEEFNKEWENGRGEFGIDF 308

[Close]

BLAST of HG10003788 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 2.5e-82
Identity = 161/258 (62.40%), Postives = 202/258 (78.29%), Query Frame = 0


Query: 28  ANPNLSFSLIGATASTYKQFIANLRKTLTLNSPTVYGIPVLASTAKGLNRFILINLTNYQ 87
           A  ++SFS++GAT+ TY+QFI NLR TLT+ SP VYGIPVL STA G  RFIL++LTNY 
Sbjct: 19  AEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVGSARFILVHLTNYN 78

Query: 88  AESITIAIDVVNVYIVAYQARNKAYFLKNVSKETHDVLFKDMKHETLPYKGNYDDLERFA 147
            ESIT+AIDVVN+Y+VAY+A N AYFL + S E ++VLFK +KH  LPYKGNYD LE  A
Sbjct: 79  TESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRLPYKGNYDGLETAA 138

Query: 148 GNISRENIDLGLSELGSSIGNLFHYKAGNSVPRALIVIIQTISEAARFKYIE---SQFVR 207
           G ISRE IDLG SE+ SSIGN+FH+  G S+ RA IVIIQ++ EAARFKYIE   S+ V+
Sbjct: 139 GKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSVLEAARFKYIEQRVSENVK 198

Query: 208 SKFKPDPGFISLENRWSDLCEQIQKAQKLGGKFKSPVPVRSVSNKPIMVSDVNSAVVKGI 267
           +KFKPDP F+SLENRWSDL EQIQ AQK  G+F   + +R+VSNKPI+V++V+S +VKGI
Sbjct: 199 TKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSSPIVKGI 258

Query: 268 ALLLHDKAAEAKNGNLKL 283
           ALLL+ K   A +  +++
Sbjct: 259 ALLLYYKVRVAIDNIIRM 276

[Close]

BLAST of HG10003788 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 2.2e-78
Identity = 159/265 (60.00%), Postives = 201/265 (75.85%), Query Frame = 0


Query: 19  VANHVDRRPANPNLSFSLIGATASTYKQFIANLRKTLTLNSPTVYGIPVLASTAKGLNRF 78
           +A ++  R A  ++SFS++G+T+ TYKQFI N+R  LT+ S  VY IPVL +TA G  RF
Sbjct: 10  LALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARF 69

Query: 79  ILINLTNYQAESITIAIDVVNVYIVAYQARNKAYFLKNVSKETHDVLFKDMKHETLPYKG 138
           +L++LTNY +ESI +AIDVVNVY+VAY+A N AYFL + S E   VLF+ + H  L YKG
Sbjct: 70  LLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKG 129

Query: 139 NYDDLERFAGNISRENIDLGLSELGSSIGNLFHYKAGNSVPRALIVIIQTISEAARFKYI 198
           NYD LE  A  ISRE IDLG SE+ SSIGN+FH+ AG SVPRA IVIIQ++SEAARFKYI
Sbjct: 130 NYDGLET-AAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYI 189

Query: 199 E---SQFVRSKFKPDPGFISLENRWSDLCEQIQKAQKLGGKFKSPVPVRSVSNKPIMVSD 258
           E   S+ V++ FKPDP F+SL+NRWSDL EQIQ AQK GG+F   V +R+VSNKPI+V+D
Sbjct: 190 EQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTD 249

Query: 259 VNSAVVKGIALLLHDKAAEAKNGNL 281
           V+S VVKGIALLL+ K +   + N+
Sbjct: 250 VSSPVVKGIALLLYYKVSVVMDDNI 273

The following BLAST results are available for this feature:

BLAST of HG10003788 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022926255.1	1.3e-106	70.82	ribosome-inactivating protein bryodin II-like [Cucurbita moschata]	[more]
XP_022981562.1	1.7e-106	70.49	ribosome-inactivating protein bryodin II-like [Cucurbita maxima]	[more]
KAG7037232.1	3.3e-105	70.16	hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023523582.1	2.1e-104	70.16	ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo]	[more]
KAG7037234.1	2.0e-102	69.18	hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of HG10003788 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	3.9e-77	61.98	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P24817	9.4e-55	48.85	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	3.6e-54	48.46	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P84531	2.0e-49	48.97	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
P09989	1.9e-47	48.57	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]

BLAST of HG10003788 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EKL0	6.5e-107	70.82	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1	[more]
A0A6J1IUB2	8.4e-107	70.49	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]
A0A6J1E093	1.9e-95	67.51	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1DWK4	2.5e-82	62.40	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1DVE7	2.2e-78	60.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]

BLAST of HG10003788 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 42..55 score: 41.17 coord: 182..202 score: 60.03 coord: 211..228 score: 41.67 coord: 90..105 score: 52.14 coord: 152..166 score: 48.6
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 40..227 e-value: 3.5E-31 score: 108.4
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 32..277
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 195..273 e-value: 6.6E-20 score: 72.8
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 31..194 e-value: 1.4E-43 score: 150.5
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 32..277
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 186..202
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 33..270

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10003788.1	HG10003788.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity