HG10003601 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003601
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRNase H domain-containing protein
LocationChr08: 3951339 .. 3951975 (+)
RNA-Seq ExpressionHG10003601
SyntenyHG10003601
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATATTATTCCTACTTGTCCTGTCTGCTCAAGTAATGTTGAATCCACCGATCACATTCTTATAAGTTGTCCTCGGACAAAACTTATATGGGATCAGATTTCGACTTCGAACCTTCTAAAGGAGGATTTGAATAATAATTTCAAGGAACGCTGGCATATGTTAAGCTTGAACTGCAACAAGGATGAGTTGAATATTTTTGCTATTGTGTTGGTCTATATGGAATGACAGAAAGAACTTCTGGTTAGGCAACTCGATTCCTGATCACAGTGTAAGAGGGAAGTGGATTTTCAAGTATTATGAAGACTTTTTAAAGTCTAGCTATTCTGCTTGAAGTTCTATACTACCAAAGGATCTGTTGAATCCTATTCAGAGAGGTGGTTGGACCAAACTGCCCCCTGATTTCTTTAAACTAAATGTTGATGCAGCTTGTGTGCAGGGTGTTCCGATTACTGGTTTGGGGGCTATAATCAGAGATTCCAAAGGACACATTACTGGTGCTGCTGCTAAGATGATTAATCTGGATTTTGATCCTCCTCTAGCCGAAGTTCTCGCTATCAGAGAAGGAATTCAGCTTGCTTCCAAATTTCACTGCTCGAATCTTATTGTGGAATCTGATTGCTCCCCAAGCTATTAA

mRNA sequence

ATGAATATTATTCCTACTTGTCCTGTCTGCTCAAGTAATGTTGAATCCACCGATCACATTCTTATAAGTTGTCCTCGGACAAAACTTATATGGGATCAGATTTCGACTTCGAACCTTCTAAAGGAGGATTTGAATAATAATTTCAAGGAACGCTGGCATATGTTAAGCTTGAACTGCAACAAGGATGAGTTGAATATTTTTGCTATTGATCTGTTGAATCCTATTCAGAGAGGTGGTTGGACCAAACTGCCCCCTGATTTCTTTAAACTAAATGTTGATGCAGCTTGTGTGCAGGGTGTTCCGATTACTGGTTTGGGGGCTATAATCAGAGATTCCAAAGGACACATTACTGGTGCTGCTGCTAAGATGATTAATCTGGATTTTGATCCTCCTCTAGCCGAAGTTCTCGCTATCAGAGAAGGAATTCAGCTTGCTTCCAAATTTCACTGCTCGAATCTTATTGTGGAATCTGATTGCTCCCCAAGCTATTAA

Coding sequence (CDS)

ATGAATATTATTCCTACTTGTCCTGTCTGCTCAAGTAATGTTGAATCCACCGATCACATTCTTATAAGTTGTCCTCGGACAAAACTTATATGGGATCAGATTTCGACTTCGAACCTTCTAAAGGAGGATTTGAATAATAATTTCAAGGAACGCTGGCATATGTTAAGCTTGAACTGCAACAAGGATGAGTTGAATATTTTTGCTATTGATCTGTTGAATCCTATTCAGAGAGGTGGTTGGACCAAACTGCCCCCTGATTTCTTTAAACTAAATGTTGATGCAGCTTGTGTGCAGGGTGTTCCGATTACTGGTTTGGGGGCTATAATCAGAGATTCCAAAGGACACATTACTGGTGCTGCTGCTAAGATGATTAATCTGGATTTTGATCCTCCTCTAGCCGAAGTTCTCGCTATCAGAGAAGGAATTCAGCTTGCTTCCAAATTTCACTGCTCGAATCTTATTGTGGAATCTGATTGCTCCCCAAGCTATTAA

Protein sequence

MNIIPTCPVCSSNVESTDHILISCPRTKLIWDQISTSNLLKEDLNNNFKERWHMLSLNCNKDELNIFAIDLLNPIQRGGWTKLPPDFFKLNVDAACVQGVPITGLGAIIRDSKGHITGAAAKMINLDFDPPLAEVLAIREGIQLASKFHCSNLIVESDCSPSY
Homology
BLAST of HG10003601 vs. NCBI nr
Match: XP_028111659.1 (uncharacterized protein LOC114309966 [Camellia sinensis])

HSP 1 Score: 82.4 bits (202), Expect = 3.9e-12
Identity = 46/152 (30.26%), Postives = 70/152 (46.05%), Query Frame = 0

Query: 7   CPVCSSNVESTDHILISCPRTKLIWDQISTSNLLKEDLNNNFKERWHMLSLNCNKDELNI 66
           CP+C S +E  +H+L  CP TK +W   + +N   +      + R+H LS N      ++
Sbjct: 451 CPICQSEIEIVEHLLFDCPWTKAVWSAQTCNNATSQ------QSRYHRLSQN------SV 510

Query: 67  FAIDLLNPIQRGGWTKLPPDFFKLNVDAACVQGVPITGLGAIIRDSKGHITGAAAKMINL 126
             ++  +P     W+      FK+N DAA  Q      + AIIRDS GH     A+ +  
Sbjct: 511 PPLESTSPEPSSAWSPAAHGCFKINCDAAFCQRTSKAAVAAIIRDSNGHFINGNARTLGA 570

Query: 127 DFDPPLAEVLAIREGIQLASKFHCSNLIVESD 159
                L E LA+R    +      SN+ +ESD
Sbjct: 571 S-SAILTEALAVRMACLMIQAHKLSNVEIESD 589

BLAST of HG10003601 vs. NCBI nr
Match: XP_026459229.1 (uncharacterized protein LOC113359871 [Papaver somniferum])

HSP 1 Score: 79.0 bits (193), Expect = 4.3e-11
Identity = 55/180 (30.56%), Postives = 79/180 (43.89%), Query Frame = 0

Query: 2   NIIPTCPVCSSNVESTDHILISCPRTKLIW------------DQISTSNLLKEDLNNNFK 61
           NI   CP+C S  E+  H+LI C     IW             QIS S  +    +++  
Sbjct: 10  NISAVCPLCESQEETLQHLLIECDYANAIWPGMNINVHSLHSQQISVSRWIASWFSDSDT 69

Query: 62  E----RWHMLSLN----CNKDELNIFAIDLLNPIQRGGWTKLPPDFFKLNVDAACVQGVP 121
           +    RW  + +N    C K+E     +   N IQ   WT  P    K+N+DA+      
Sbjct: 70  DDENLRWKTILINLVDFCLKEETQY--LKGKNNIQPQQWTPPPTSHLKINIDASFDHNTK 129

Query: 122 ITGLGAIIRDSKGHITGAAAKMINLDFDPPLAEVLAIREGIQLASKFHC--SNLIVESDC 160
             G+G IIRDS G   G   +  +   DP   E LA++  I  A + +   SNL+ E+DC
Sbjct: 130 EIGIGLIIRDSAGSAKGIRGRYYHGGVDPEQTECLAMKHAILWAKELNLQFSNLLFEADC 187

BLAST of HG10003601 vs. NCBI nr
Match: KAF7838458.1 (reverse transcriptase [Senna tora])

HSP 1 Score: 78.6 bits (192), Expect = 5.7e-11
Identity = 55/178 (30.90%), Postives = 86/178 (48.31%), Query Frame = 0

Query: 1   MNIIPTCPVCSSNVESTDHILISCPRTKLIWDQISTSNLLKEDLNNNFKERWHMLSLNCN 60
           +NI+  CPVCS  VES DH+  SC  T+ +W+ ++ +  + +  + +  ++W  L LN  
Sbjct: 513 LNIV--CPVCSVGVESLDHLFASCFETRKVWEVMNVNPTILD--SGSCFQKW--LKLNAG 572

Query: 61  KDELN---------------IFAIDLLNPIQRG-----GWTKLPPDFFKLNVDAACVQGV 120
             + +               ++     +P +       GW   P  ++KLN D +C    
Sbjct: 573 NKKFDCLRVGRRAVCKAVEFVYLNSKYSPERAVETVFIGWQPPPLGWWKLNTDGSCQNN- 632

Query: 121 PITGLGAIIRDSKGHITGAAAKMINLDFDPPLAEVLAIREGIQLASKFHCSNLIVESD 159
            + G G IIRD+ G+      K +  + D  LAE+ AI EG+ LA   HC  LIVESD
Sbjct: 633 -LIGGGGIIRDANGNWIHGFKKFLG-EGDCLLAELWAIVEGVNLAKHLHCDKLIVESD 681

BLAST of HG10003601 vs. NCBI nr
Match: KAE8772052.1 (hypothetical protein D1007_55969 [Hordeum vulgare])

HSP 1 Score: 78.2 bits (191), Expect = 7.4e-11
Identity = 53/201 (26.37%), Postives = 79/201 (39.30%), Query Frame = 0

Query: 7   CPVCSSNVESTDHILISCPRTKLIWDQISTSNLLKED-LNNNFKERWHMLSLNCNKDELN 66
           CP C    +S  H L  C R+  +W Q+   +++K+        E   +  L+ +  E+N
Sbjct: 143 CPECKLGPDSIRHFLFECQRSCEVWRQLGLYDIIKKKCAKYKSGEEVVLELLHMDASEIN 202

Query: 67  IFAIDLLN------------------------------------------------PIQR 126
           I     L                                                  I+ 
Sbjct: 203 ILGCPNLREMVATTAWYLWFEHRKIYHGEEIQSASRIALAIRGLSANFTIACAHNAKIRS 262

Query: 127 GGWTKLPPDFFKLNVDAACVQGVPITGLGAIIRDSKGHITGAAAKMINLDFDPPLAEVLA 159
           GGW K P  + KLNVDA   Q +    +GAI+RD  G    AA + I + +DP +AE LA
Sbjct: 263 GGWEKPPKGYVKLNVDAGFDQDLLQGSVGAIVRDQNGQFIAAANEKIEICYDPNMAEALA 322

BLAST of HG10003601 vs. NCBI nr
Match: XP_030926547.1 (uncharacterized protein LOC115953156 [Quercus lobata])

HSP 1 Score: 78.2 bits (191), Expect = 7.4e-11
Identity = 50/195 (25.64%), Postives = 77/195 (39.49%), Query Frame = 0

Query: 7   CPVCSSNVESTDHILISCPRTKLIWDQISTSNLLKEDLNNNFKERWHMLSLNCNKDELNI 66
           CPVC ++ ES DH L+ C  + L+WD    + L  +   N+F +    +  +    +L I
Sbjct: 281 CPVCGNDAESVDHALLRCDFSSLVWDFWLENPLHTQGFKNSFLDSALFILSHSTLQDLEI 340

Query: 67  F-------------------------------------------AIDLLNPIQRGGWTKL 126
           F                                             DL+ P     W+  
Sbjct: 341 FFATAWAIWSNRNNILHKDGGLSPLHVWHRARNVVEEFTCSASWDFDLVRPAS-SSWSPP 400

Query: 127 PPDFFKLNVDAACVQGVPITGLGAIIRDSKGHITGAAAKMINLDFDPPLAEVLAIREGIQ 159
           PP  FK+NVD A  +    + +G +IRDS G +  A    +   F   L+EV A+ +G+ 
Sbjct: 401 PPGAFKVNVDGASSEQEGSSSMGVVIRDSNGQVVAALCLPLQSYFSAELSEVFALEQGVL 460

BLAST of HG10003601 vs. ExPASy TrEMBL
Match: M5X848 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G063400 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 4.7e-11
Identity = 48/167 (28.74%), Postives = 81/167 (48.50%), Query Frame = 0

Query: 7   CPVCSSNVESTDHILISCPRTKLIWDQISTSNLLKEDLNNNFKERWHMLSLNCNKDELNI 66
           CP+C  N E+  H++ SCP  + +W ++    + K    + + + +      C+K E  I
Sbjct: 145 CPICIVNSENLIHVVWSCPGAQKVWKKVRFMEVFKGLQLSTYGDFFEA----CSKKEEMI 204

Query: 67  F--AIDLLNPI-------------QRGGWTKLPPDFFKLNVDAACVQGVPITGLGAIIRD 126
           F  A+ L                 +   W+  P   FKLNVDAA +    + G+GA+IR+
Sbjct: 205 FNGAVHLTEEYGDLMRRESNIVIEKASKWSHPPVGKFKLNVDAAYIPDTGVGGIGAVIRN 264

Query: 127 SKGHITGAAAKMINLDFDPPLAEVLAIREGIQLASKFHCSNLIVESD 159
            KG +  A A  ++    P  AE++A++ G+  A     S+++VESD
Sbjct: 265 EKGEVMVATALPLHTATSPKHAEIMALQFGLNFAWDAGFSSILVESD 307

BLAST of HG10003601 vs. ExPASy TrEMBL
Match: A0A453EWI8 (Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 6.1e-11
Identity = 54/207 (26.09%), Postives = 92/207 (44.44%), Query Frame = 0

Query: 1   MNIIPTCPVCSSNVESTDHILISCPRTKLIWDQISTSNLL-------------------- 60
           M + P+CP CS  +E T H+L  C + K +W ++    ++                    
Sbjct: 242 MKVSPSCPTCSRGLEDTKHMLFLCSKAKEVWKRLGMDVIIGKACEVDLAGEAILEYLLLL 301

Query: 61  -KEDLN----NNFKE-----RWHM-----------LSLNCNKDELNIFAI--DLLN---- 120
             +DL+     N +E      W++           L+ N N+  + I A+  + +N    
Sbjct: 302 PDQDLSIMGYQNVREMIAISAWYLWWERRKLVHKELTQNANQIAMGIIALTSNFVNASSP 361

Query: 121 --PIQRGGWTKLPPDFFKLNVDAACVQGVPITGLGAIIRDSKGHITGAAAKMINLDFDPP 159
              +++GGW   P  F KLNVDA+    +    +GA++RD KG         I+   D  
Sbjct: 362 KATMKKGGWYCPPRGFVKLNVDASFDHDMLKGTMGAVLRDHKGRFIAGGNGKIDYCADVL 421

BLAST of HG10003601 vs. ExPASy TrEMBL
Match: A0A2K2CVF0 (RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_3g05652v3 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.4e-10
Identity = 37/84 (44.05%), Postives = 49/84 (58.33%), Query Frame = 0

Query: 75  IQRGGWTKLPPDFFKLNVDAACVQGVPITGLGAIIRDSKGHITGAAAKMINLDFDPPLAE 134
           IQR GW + P    KLNVD +  + +     GA+IRD+ G   GA+   I + +D   AE
Sbjct: 115 IQRHGWQRPPYGMQKLNVDTSFTEDMDEGATGAVIRDASGMFVGASNSFIPIVYDAATAE 174

Query: 135 VLAIREGIQLASKFHCSNLIVESD 159
            LA+  GIQLA  F CSNL++ SD
Sbjct: 175 ALALWHGIQLAKNFGCSNLLINSD 198

BLAST of HG10003601 vs. ExPASy TrEMBL
Match: A0A0Q3GGJ7 (RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_2g53033v3 PE=4 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 2.3e-10
Identity = 52/201 (25.87%), Postives = 78/201 (38.81%), Query Frame = 0

Query: 7   CPVCSSNVESTDHILISCPRTKLIWDQISTSNLLKEDLNNN------------------- 66
           C  C + VE   H+L +C R K +W  +    L+ + L  +                   
Sbjct: 22  CVTCPAQVEDLRHVLFTCSRAKAVWSALGAGELIAQSLALDRAGSAVLEDLLAGDHASKI 81

Query: 67  -------------------FKERWHMLSLNCNKDELNIFAIDLL-----------NPIQR 126
                              ++ R  M        +   F+I  L           + IQR
Sbjct: 82  YMTPVTFGELCAVACWYIWWERRQLMHGEPIPPPDTTAFSIQALVMNYVRSYSKQSGIQR 141

Query: 127 GGWTKLPPDFFKLNVDAACVQGVPITGLGAIIRDSKGHITGAAAKMINLDFDPPLAEVLA 159
            GW K P    KLNVDA+ ++       GA+IRD+ G    A+   I + +D  +AE +A
Sbjct: 142 LGWQKPPEGMQKLNVDASFIEDKEEGATGAVIRDASGLFVRASNSCIPIVYDATMAEAMA 201

BLAST of HG10003601 vs. ExPASy TrEMBL
Match: A0A7H4L9Z4 (Genome assembly, chromosome: II OS=Triticum aestivum OX=4565 GN=CAMPLR22A2D_LOCUS20 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.3e-10
Identity = 37/93 (39.78%), Postives = 53/93 (56.99%), Query Frame = 0

Query: 66  IFAIDLLNPIQRGGWTKLPPDFFKLNVDAACVQGVPITGLGAIIRDSKGHITGAAAKMIN 125
           + A D    +++ GW K P  F KLNVDAA         +GA+IRD +GH   +  K+I 
Sbjct: 6   VIACDPKAVLKKDGWRKPPNGFAKLNVDAAFDYDALHGAMGAVIRDDRGHFIVSGNKLIE 65

Query: 126 LDFDPPLAEVLAIREGIQLASKFHCSNLIVESD 159
             +DP   E LA++ G+QL+S   C+ L+V SD
Sbjct: 66  SCYDPLSVEALALKFGLQLSSSAGCNRLVVNSD 98

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_028111659.13.9e-1230.26uncharacterized protein LOC114309966 [Camellia sinensis][more]
XP_026459229.14.3e-1130.56uncharacterized protein LOC113359871 [Papaver somniferum][more]
KAF7838458.15.7e-1130.90reverse transcriptase [Senna tora][more]
KAE8772052.17.4e-1126.37hypothetical protein D1007_55969 [Hordeum vulgare][more]
XP_030926547.17.4e-1125.64uncharacterized protein LOC115953156 [Quercus lobata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
M5X8484.7e-1128.74Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G063400 PE=4 SV=1[more]
A0A453EWI86.1e-1126.09Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=4 S... [more]
A0A2K2CVF01.4e-1044.05RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_3... [more]
A0A0Q3GGJ72.3e-1025.87RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_2... [more]
A0A7H4L9Z42.3e-1039.78Genome assembly, chromosome: II OS=Triticum aestivum OX=4565 GN=CAMPLR22A2D_LOCU... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 91..161
e-value: 8.3E-13
score: 48.2
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 48..160
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 90..160
e-value: 2.09257E-16
score: 68.88
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 86..159

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003601.1HG10003601.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity