HG10003559 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003559
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	Chr08: 3266742 .. 3267821 (+)
RNA-Seq Expression	HG10003559
Synteny	HG10003559

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCTATTTAGAAAACGAAGAGCATAAAAAGGAATGCATGGAAGGAAGTGAAGAAGAAGAAGGATTGGAAATGTCTTCGTCCAGTGTTTGTTCCGAAATCTCAACCCAACAACTTCCATGGAAGCACAGAATTATTCTTCGCTTCGCCGCTCTACTCTTCAACACTTCTCTCCGATCAGATCTCACCGTCAATCGTTGGCTTCTCAATTTCATCGACCCCAAAATCCCTCCCAATCCCAATTCAATCCACGCCGTTTCCTCTTCCGATCTCACTATCGACAGTTCTCGTCACCTCTTCCTTCGAATCTTAACCCCCACCGCCGCCGCCGACTCTCTCTCCCCGCCCCTCATTTTCTACTTCCACGGCGGCGGCTTCGCCTTCGGTTCCCCCGACGCCACTTCAATGGACATGGCTGCCCGCCGCTTCGCCAAAGAACTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCACTCCTGAGTTCCGATTCCCCTGCCAATACGACGACGGATTCGACGCTTTGAAATTCATTGACGAAATGGACGACGGCGCATTGCTGGGGAGGGCAGACCTGAGCCGTTGCTTTATTTTGGGGGAAAGTGCCGGGGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGAGTTCAAGAGAGTGAAGGTGGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGTGGAGAGGAAAGAACAGAGTCGGAGAATCGACTGTGTAAGCAGCTTCCGTTGACTCTGAACATGACGGATTGGTTCTGGAGGGCGTTTTTGCCGGCCGGGGAAAATCGAGACCACGCGGCGGCGAATGTGAATGGACCGAATGGAAGGGATATATCGGGAGTGAAAAATTTTCCGGCGACGGTGATTTTTGGCGGAGGATTAGATCTGCTGATTGATCGACAGAAGTGTTACTATGAGGGGCTGAAGAGAATGGGAAAGAATGTGAAGTTGGTGGTGTTTTCGAAGGCCATCCATGGATTCTTTGGCTTTGCGGATCTGCCGGAATATTCTTTAATGATTAAGGAGATGAAGGATTTCATTGCAAACCTCAGATAG

mRNA sequence

ATGATCTATTTAGAAAACGAAGAGCATAAAAAGGAATGCATGGAAGGAAGTGAAGAAGAAGAAGGATTGGAAATGTCTTCGTCCAGTGTTTGTTCCGAAATCTCAACCCAACAACTTCCATGGAAGCACAGAATTATTCTTCGCTTCGCCGCTCTACTCTTCAACACTTCTCTCCGATCAGATCTCACCGTCAATCGTTGGCTTCTCAATTTCATCGACCCCAAAATCCCTCCCAATCCCAATTCAATCCACGCCGTTTCCTCTTCCGATCTCACTATCGACAGTTCTCGTCACCTCTTCCTTCGAATCTTAACCCCCACCGCCGCCGCCGACTCTCTCTCCCCGCCCCTCATTTTCTACTTCCACGGCGGCGGCTTCGCCTTCGGTTCCCCCGACGCCACTTCAATGGACATGGCTGCCCGCCGCTTCGCCAAAGAACTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCACTCCTGAGTTCCGATTCCCCTGCCAATACGACGACGGATTCGACGCTTTGAAATTCATTGACGAAATGGACGACGGCGCATTGCTGGGGAGGGCAGACCTGAGCCGTTGCTTTATTTTGGGGGAAAGTGCCGGGGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGAGTTCAAGAGAGTGAAGGTGGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGTGGAGAGGAAAGAACAGAGTCGGAGAATCGACTGTGTAAGCAGCTTCCGTTGACTCTGAACATGACGGATTGGTTCTGGAGGGCGTTTTTGCCGGCCGGGGAAAATCGAGACCACGCGGCGGCGAATGTGAATGGACCGAATGGAAGGGATATATCGGGAGTGAAAAATTTTCCGGCGACGGTGATTTTTGGCGGAGGATTAGATCTGCTGATTGATCGACAGAAGTGTTACTATGAGGGGCTGAAGAGAATGGGAAAGAATGTGAAGTTGGTGGTGTTTTCGAAGGCCATCCATGGATTCTTTGGCTTTGCGGATCTGCCGGAATATTCTTTAATGATTAAGGAGATGAAGGATTTCATTGCAAACCTCAGATAG

Coding sequence (CDS)

ATGATCTATTTAGAAAACGAAGAGCATAAAAAGGAATGCATGGAAGGAAGTGAAGAAGAAGAAGGATTGGAAATGTCTTCGTCCAGTGTTTGTTCCGAAATCTCAACCCAACAACTTCCATGGAAGCACAGAATTATTCTTCGCTTCGCCGCTCTACTCTTCAACACTTCTCTCCGATCAGATCTCACCGTCAATCGTTGGCTTCTCAATTTCATCGACCCCAAAATCCCTCCCAATCCCAATTCAATCCACGCCGTTTCCTCTTCCGATCTCACTATCGACAGTTCTCGTCACCTCTTCCTTCGAATCTTAACCCCCACCGCCGCCGCCGACTCTCTCTCCCCGCCCCTCATTTTCTACTTCCACGGCGGCGGCTTCGCCTTCGGTTCCCCCGACGCCACTTCAATGGACATGGCTGCCCGCCGCTTCGCCAAAGAACTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCACTCCTGAGTTCCGATTCCCCTGCCAATACGACGACGGATTCGACGCTTTGAAATTCATTGACGAAATGGACGACGGCGCATTGCTGGGGAGGGCAGACCTGAGCCGTTGCTTTATTTTGGGGGAAAGTGCCGGGGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGAGTTCAAGAGAGTGAAGGTGGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGTGGAGAGGAAAGAACAGAGTCGGAGAATCGACTGTGTAAGCAGCTTCCGTTGACTCTGAACATGACGGATTGGTTCTGGAGGGCGTTTTTGCCGGCCGGGGAAAATCGAGACCACGCGGCGGCGAATGTGAATGGACCGAATGGAAGGGATATATCGGGAGTGAAAAATTTTCCGGCGACGGTGATTTTTGGCGGAGGATTAGATCTGCTGATTGATCGACAGAAGTGTTACTATGAGGGGCTGAAGAGAATGGGAAAGAATGTGAAGTTGGTGGTGTTTTCGAAGGCCATCCATGGATTCTTTGGCTTTGCGGATCTGCCGGAATATTCTTTAATGATTAAGGAGATGAAGGATTTCATTGCAAACCTCAGATAG

Protein sequence

MIYLENEEHKKECMEGSEEEEGLEMSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIHAVSSSDLTIDSSRHLFLRILTPTAAADSLSPPLIFYFHGGGFAFGSPDATSMDMAARRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGESAGGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKNVKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIANLR

Homology

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BLAST of HG10003559 vs. NCBI nr
Match: XP_004141618.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein Csa_008680 [Cucumis sativus])

HSP 1 Score: 578.9 bits (1491), Expect = 2.9e-161
Identity = 284/338 (84.02%), Postives = 303/338 (89.64%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M SSSVC E+ST QLPWKH++ LRFA LLFNTSLRSD TVNR LL F+DPKIPPNPNS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 85  AVSSSDLTIDSSRHLFLRILT--PTAAADSLSP--PLIFYFHGGGFAFGSPDATSMDMAA 144
           +VSSSDLTID+SR LFLRI T  PTAA D   P  P+IFYFHGGGFAFGS DATS DMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 145 RRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGE 204
           R FA++LRAVVISVNYRL PEFRFPCQYDDGFDALKFIDEMDD +LL R DLSRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 205 SAGGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 264
           SAGGNLGHHVAVRASEYEFKRVK++GFIASQPFFGG+ERTESENRLCKQLPLTL MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 265 WRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMG 324
           WRAFLPAGE+RDHAAANVNGPNGRDISG++NFPATVIF GGLDLL+DRQK YYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 325 KNVKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIANL 359
           K+VKLVVFS A HGFFGF DLPEYSLMI+EM DFIA L
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

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BLAST of HG10003559 vs. NCBI nr
Match: XP_008459713.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 578.2 bits (1489), Expect = 5.0e-161
Identity = 283/336 (84.23%), Postives = 304/336 (90.48%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M SS VC +IST QLPWKHRI LRFA LL+NTSLRSDLTVNR LLNFIDPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 85  AVSSSDLTIDSSRHLFLRIL--TPTAAADSLSP--PLIFYFHGGGFAFGSPDATSMDMAA 144
           +VSSSDL +D+SR LFLRI   TPTAA D   P  P+IFYFHGGGF FGS DATS DMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 145 RRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGE 204
           RRFAK+LRAVVISVNYRL PEFRFPCQYDDGFDALKFIDEMDD +LL R DLS CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 205 SAGGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 264
           SAGGNLGHHVAVRASEYEFKRVK++GFIASQPFFGG+ERTESENRLCKQLPLTL+MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 265 WRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMG 324
           WRAFLPAGE+RDHAAANVNGPNGRDISG++NFPAT+IF GGLDLLIDRQKCYYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 325 KNVKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIA 357
           K+VKLVVFS A+HG+FGFADLPEYSLMIKEM+DFIA
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIA 336

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BLAST of HG10003559 vs. NCBI nr
Match: KAA0039340.1 (putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00523.1 putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 571.2 bits (1471), Expect = 6.1e-159
Identity = 280/336 (83.33%), Postives = 301/336 (89.58%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M SS VC +IST QLPWKHRI LRFA LL+NTSLRSDLTVNR LLNFIDPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 85  AVSSSDLTIDSSRHLFLRIL--TPTAAADSLSP--PLIFYFHGGGFAFGSPDATSMDMAA 144
           +VSSSDL +D+SR LFLRI   TPTAA D   P  P+IFYFHGGGF FGS DATS DMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 145 RRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGE 204
           RRFAK+L AVVISVNYRL PEFRFPCQ DDGFD LKFIDEMDD +LL R DLSRCFILGE
Sbjct: 121 RRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 205 SAGGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 264
           SAGGNLGHHVAVRASEYEFKRVK++GFIASQPFFGG+ERTESENRLCKQLPLTL+MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 265 WRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMG 324
           WRAFLPAGE+RDHA ANVNGPNGRDISG++NFPAT+IF GGLDLLIDRQKCYYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 325 KNVKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIA 357
           K+VKLVVFS A+HG+FGFADLPEYSLMIKEM+DFIA
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIA 336

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BLAST of HG10003559 vs. NCBI nr
Match: XP_022925491.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 569.7 bits (1467), Expect = 1.8e-158
Identity = 278/334 (83.23%), Postives = 299/334 (89.52%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M  SSV SEIST QLPWKHR+ LRFAALLFN S+RSDLTVNR LLNF+DPKIPPNPNSIH
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 85  AVSSSDLTIDSSRHLFLRILTPT-AAADSLSPPLIFYFHGGGFAFGSPDATSMDMAARRF 144
           AVSSSDLTID SR LFLRI TPT AA DS+ PP+IFYFHGGGFAFGS DATSMDMAARRF
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSADATSMDMAARRF 120

Query: 145 AKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEM-DDGALLGRADLSRCFILGESA 204
           +KEL AVVISVNYRL P+FRFPCQYDDGFDA+KFIDEM DD  LLG+ADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180

Query: 205 GGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWR 264
           GGNLGHHVAVRASEY FKRVKV+GFIASQPFFGGEERTESE RLC Q PLTL + DWFW+
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 265 AFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKN 324
           AFLPAGE+RDHAAANVNGPNGRD+SG++NFPAT+I  GGLDLLIDRQ+ YYE LKRMGK 
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKE 300

Query: 325 VKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIA 357
           VKLVVF+  +HGFF F D PEYSLMIKEM+DFIA
Sbjct: 301 VKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

[Close]

BLAST of HG10003559 vs. NCBI nr
Match: XP_022973499.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 567.0 bits (1460), Expect = 1.2e-157
Identity = 277/333 (83.18%), Postives = 299/333 (89.79%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M   SV SEIST QLPWKHR+ LRFAALLFN S+RSDLTVNR LLNF+DPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 85  AVSSSDLTIDSSRHLFLRILTPT-AAADSLSPPLIFYFHGGGFAFGSPDATSMDMAARRF 144
           AVSSSDLTID+SR LFLRI TPT AA DSL PP+IFYFHGGGFAFGS  ATSMDMAARRF
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPTVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120

Query: 145 AKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDD-GALLGRADLSRCFILGESA 204
           +KEL AVVISVNYRL+P+FRFPCQYDDGFDA+KFIDEMDD   LLGRADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESA 180

Query: 205 GGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWR 264
           GGNLGHHVAVRASEY FKRVKV+GFIASQPFFGGEERTESE RLC Q PLTL + DWFW+
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 265 AFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKN 324
           AFLPAGE+RDHAAANVNGPNGRD+SG++NFPAT+I  GGLD LIDRQ+ YYE LKRMGK+
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKD 300

Query: 325 VKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFI 356
           VKLVVF+  +HGFF F DLPEYSLMIKEM+DFI
Sbjct: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 333

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BLAST of HG10003559 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.9e-80
Identity = 153/320 (47.81%), Postives = 202/320 (63.12%), Query Frame = 0


Query: 39  LPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIHAVSSSDLTIDSSRH 98
           LP K RI L   + + + + R D T+NR  L   D + PPNP  ++ VS+SD  +D SR 
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 99  LFLRILTPTAAADSLSPPLIFYFHGGGFAFGSPDATSMDMAARRFAKELRAVVISVNYRL 158
           L+ R+ TP  + D +  P++ +FHGGGFAF SP+A   D   RRFA++L A VISVNYRL
Sbjct: 73  LWFRLYTPHVSGDKI--PVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRL 132

Query: 159 TPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGESAGGNLGHHVAV---RAS 218
            PE R+P QYDDGFDALK+I+E     L   ADLSRCF  G+SAGGN+ H+VA+   R  
Sbjct: 133 APEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICREP 192

Query: 219 EYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAA 278
              F  VK++G I+ QPFFGGEERTE+E +L     ++ + TDW W+A    G NRDH A
Sbjct: 193 RSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRDHEA 252

Query: 279 ANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKNVKLVVFSKAIHGF 338
            NV GPN  DISG+ ++P T++   G D L D Q+ YYE LK  GK   L+ +    H F
Sbjct: 253 VNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFHAF 312

Query: 339 FGFADLPEYSLMIKEMKDFI 356
           + F +LPE   +I  +KDF+
Sbjct: 313 YIFPELPEAGQLIMRIKDFV 326

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BLAST of HG10003559 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.4e-57
Identity = 135/355 (38.03%), Postives = 192/355 (54.08%), Query Frame = 0


Query: 14  MEGSEEEEGLEMSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFID 73
           M GSEE   +E          S   +P    +++    L +N   R D T NR L  F+D
Sbjct: 1   MAGSEEVNLIE----------SKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLD 60

Query: 74  PKIPPNPNSIHAVSSSDLTIDSSRHLFLRILTPTAAADSLS------------PPLIFYF 133
            K+P N N ++ V S D+ ID   +L  R+  P  A  S S             P+I +F
Sbjct: 61  RKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFF 120

Query: 134 HGGGFAFGSPDATSMDMAARRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEM 193
           HGG FA  S ++   D   RR      AVV+SVNYR  PE R+PC YDDG+  LK+++  
Sbjct: 121 HGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVN-- 180

Query: 194 DDGALLGRADLS-RCFILGESAGGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERT 253
               L  + D   R F+ G+S+GGN+ H+VAVRA E    R+ V+G I   P FGG ERT
Sbjct: 181 SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERT 240

Query: 254 ESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGG 313
           ESE RL  +  +T+   DW+WRAFLP GE+R+H A +  GP  + + G+ +FP +++   
Sbjct: 241 ESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVA 300

Query: 314 GLDLLIDRQKCYYEGLKRMGKNVKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFI 356
           GLDL+ D Q  Y EGLK+ G+ VKL+   +A  GF+   +   +  ++ E+  F+
Sbjct: 301 GLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

[Close]

BLAST of HG10003559 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.7e-56
Identity = 132/329 (40.12%), Postives = 179/329 (54.41%), Query Frame = 0


Query: 52  LLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIHAVSSSDLTIDSSRHLFLRILTPTAAAD 111
           L +N   R+D T  R L  ++D ++P N   +  VSS D  ID S  L +RI    A  D
Sbjct: 29  LSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGD 88

Query: 112 --------------------SLSP-PLIFYFHGGGFAFGSPDATSMDMAARRFAKELRAV 171
                               +  P P+I +FHGG F   S  +T  D   RRF K  + V
Sbjct: 89  AEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGV 148

Query: 172 VISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGESAGGNLGHHV 231
           V+SVNYR  PE R+PC YDDG+ ALK++         G A  +R F+ G+S+GGN+ HHV
Sbjct: 149 VVSVNYRRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQ-ARVFLSGDSSGGNIAHHV 208

Query: 232 AVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGEN 291
           AVRA++   + VKV G I     FGG ERTESE RL  +  +TL   DW+W+A+LP   +
Sbjct: 209 AVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDAD 268

Query: 292 RDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKNVKLVVFSK 351
           RDH A N  GPNGR + G+  F  ++I   GLDL  DRQ  Y + L+  G +VK+V    
Sbjct: 269 RDHPACNPFGPNGRRLGGLP-FAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCEN 328

Query: 352 AIHGFFGFADLPEYSLMIKEMKDFI-ANL 359
           A  GF+   +   Y  +++E+ DF+ ANL
Sbjct: 329 ATVGFYLLPNTVHYHEVMEEISDFLNANL 352

[Close]

BLAST of HG10003559 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 7.3e-54
Identity = 120/332 (36.14%), Postives = 177/332 (53.31%), Query Frame = 0


Query: 39  LPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIHAVSSSDLTIDSSRH 98
           +P    +++    + +N   R D T NR L  ++D K+  N N +  V S D+ ID   +
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 99  LFLRILTPTAAADSLSP--------------PLIFYFHGGGFAFGSPDATSMDMAARRFA 158
           L  R+  P  A     P              P+I +FHGG FA  S ++   D   RR  
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 159 KELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLS-RCFILGESAG 218
              + VV+SVNYR  PE  +PC YDDG+ AL +++      L  + D     F+ G+S+G
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSG 195

Query: 219 GNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRA 278
           GN+ H+VA+RA E     + V+G I   P FGG ERTESE  L  +  +T+   DW+W+A
Sbjct: 196 GNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKA 255

Query: 279 FLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKNV 338
           FLP GE+R+H A N   P G+ + GV +FP +++   GLDL+ D Q  Y EGLK+ G+ V
Sbjct: 256 FLPEGEDREHPACNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEV 315

Query: 339 KLVVFSKAIHGFFGFADLPEYSLMIKEMKDFI 356
           KL+   KA  GF+   +   +  ++ E+  F+
Sbjct: 316 KLMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

[Close]

BLAST of HG10003559 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.4e-52
Identity = 117/335 (34.93%), Postives = 183/335 (54.63%), Query Frame = 0


Query: 39  LPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIHAVSSSDLTIDSSRH 98
           +P    +++    L +    R D + NR L  F+D K+P N   +  V S D  +DS+ +
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTN 75

Query: 99  LFLRILTPTA--------AADSLSP-------PLIFYFHGGGFAFGSPDATSMDMAARRF 158
           L  RI  P +          +   P       P++ +FHGG F   S ++   D   RR 
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 159 AKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGESAG 218
                 VV+SV+YR +PE R+PC YDDG++AL ++ +       G+      ++ G+S+G
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWV-KSRVWLQSGKDSNVYVYLAGDSSG 195

Query: 219 GNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRA 278
           GN+ H+VAVRA+    + VKV+G I   P FGG+ERT+SE  L  +  +T+   DW+WRA
Sbjct: 196 GNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRA 255

Query: 279 FLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKNV 338
           +LP GE+RDH A N  GP G+ + GV NFP +++   GLDL+ D Q  Y +GLK+ G  V
Sbjct: 256 YLPEGEDRDHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEV 315

Query: 339 KLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIANL 359
            L+   +A  GF+   +   +  +++E+  F+ ++
Sbjct: 316 NLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

[Close]

BLAST of HG10003559 vs. ExPASy TrEMBL
Match: A0A0A0KXS8 (Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.4e-161
Identity = 284/338 (84.02%), Postives = 303/338 (89.64%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M SSSVC E+ST QLPWKH++ LRFA LLFNTSLRSD TVNR LL F+DPKIPPNPNS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 85  AVSSSDLTIDSSRHLFLRILT--PTAAADSLSP--PLIFYFHGGGFAFGSPDATSMDMAA 144
           +VSSSDLTID+SR LFLRI T  PTAA D   P  P+IFYFHGGGFAFGS DATS DMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 145 RRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGE 204
           R FA++LRAVVISVNYRL PEFRFPCQYDDGFDALKFIDEMDD +LL R DLSRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 205 SAGGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 264
           SAGGNLGHHVAVRASEYEFKRVK++GFIASQPFFGG+ERTESENRLCKQLPLTL MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 265 WRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMG 324
           WRAFLPAGE+RDHAAANVNGPNGRDISG++NFPATVIF GGLDLL+DRQK YYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 325 KNVKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIANL 359
           K+VKLVVFS A HGFFGF DLPEYSLMI+EM DFIA L
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

[Close]

BLAST of HG10003559 vs. ExPASy TrEMBL
Match: A0A1S3CBA4 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.4e-161
Identity = 283/336 (84.23%), Postives = 304/336 (90.48%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M SS VC +IST QLPWKHRI LRFA LL+NTSLRSDLTVNR LLNFIDPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 85  AVSSSDLTIDSSRHLFLRIL--TPTAAADSLSP--PLIFYFHGGGFAFGSPDATSMDMAA 144
           +VSSSDL +D+SR LFLRI   TPTAA D   P  P+IFYFHGGGF FGS DATS DMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 145 RRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGE 204
           RRFAK+LRAVVISVNYRL PEFRFPCQYDDGFDALKFIDEMDD +LL R DLS CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 205 SAGGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 264
           SAGGNLGHHVAVRASEYEFKRVK++GFIASQPFFGG+ERTESENRLCKQLPLTL+MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 265 WRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMG 324
           WRAFLPAGE+RDHAAANVNGPNGRDISG++NFPAT+IF GGLDLLIDRQKCYYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 325 KNVKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIA 357
           K+VKLVVFS A+HG+FGFADLPEYSLMIKEM+DFIA
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIA 336

[Close]

BLAST of HG10003559 vs. ExPASy TrEMBL
Match: A0A5D3BL91 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001330 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 3.0e-159
Identity = 280/336 (83.33%), Postives = 301/336 (89.58%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M SS VC +IST QLPWKHRI LRFA LL+NTSLRSDLTVNR LLNFIDPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 85  AVSSSDLTIDSSRHLFLRIL--TPTAAADSLSP--PLIFYFHGGGFAFGSPDATSMDMAA 144
           +VSSSDL +D+SR LFLRI   TPTAA D   P  P+IFYFHGGGF FGS DATS DMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 145 RRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGE 204
           RRFAK+L AVVISVNYRL PEFRFPCQ DDGFD LKFIDEMDD +LL R DLSRCFILGE
Sbjct: 121 RRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 205 SAGGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWF 264
           SAGGNLGHHVAVRASEYEFKRVK++GFIASQPFFGG+ERTESENRLCKQLPLTL+MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 265 WRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMG 324
           WRAFLPAGE+RDHA ANVNGPNGRDISG++NFPAT+IF GGLDLLIDRQKCYYE LKRMG
Sbjct: 241 WRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 325 KNVKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIA 357
           K+VKLVVFS A+HG+FGFADLPEYSLMIKEM+DFIA
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIA 336

[Close]

BLAST of HG10003559 vs. ExPASy TrEMBL
Match: A0A6J1ECC8 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 8.6e-159
Identity = 278/334 (83.23%), Postives = 299/334 (89.52%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M  SSV SEIST QLPWKHR+ LRFAALLFN S+RSDLTVNR LLNF+DPKIPPNPNSIH
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 85  AVSSSDLTIDSSRHLFLRILTPT-AAADSLSPPLIFYFHGGGFAFGSPDATSMDMAARRF 144
           AVSSSDLTID SR LFLRI TPT AA DS+ PP+IFYFHGGGFAFGS DATSMDMAARRF
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSADATSMDMAARRF 120

Query: 145 AKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEM-DDGALLGRADLSRCFILGESA 204
           +KEL AVVISVNYRL P+FRFPCQYDDGFDA+KFIDEM DD  LLG+ADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180

Query: 205 GGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWR 264
           GGNLGHHVAVRASEY FKRVKV+GFIASQPFFGGEERTESE RLC Q PLTL + DWFW+
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 265 AFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKN 324
           AFLPAGE+RDHAAANVNGPNGRD+SG++NFPAT+I  GGLDLLIDRQ+ YYE LKRMGK 
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKE 300

Query: 325 VKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIA 357
           VKLVVF+  +HGFF F D PEYSLMIKEM+DFIA
Sbjct: 301 VKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

[Close]

BLAST of HG10003559 vs. ExPASy TrEMBL
Match: A0A6J1IER7 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.6e-158
Identity = 277/333 (83.18%), Postives = 299/333 (89.79%), Query Frame = 0


Query: 25  MSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIH 84
           M   SV SEIST QLPWKHR+ LRFAALLFN S+RSDLTVNR LLNF+DPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 85  AVSSSDLTIDSSRHLFLRILTPT-AAADSLSPPLIFYFHGGGFAFGSPDATSMDMAARRF 144
           AVSSSDLTID+SR LFLRI TPT AA DSL PP+IFYFHGGGFAFGS  ATSMDMAARRF
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPTVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120

Query: 145 AKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDD-GALLGRADLSRCFILGESA 204
           +KEL AVVISVNYRL+P+FRFPCQYDDGFDA+KFIDEMDD   LLGRADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESA 180

Query: 205 GGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWR 264
           GGNLGHHVAVRASEY FKRVKV+GFIASQPFFGGEERTESE RLC Q PLTL + DWFW+
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 265 AFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKN 324
           AFLPAGE+RDHAAANVNGPNGRD+SG++NFPAT+I  GGLD LIDRQ+ YYE LKRMGK+
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKD 300

Query: 325 VKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFI 356
           VKLVVF+  +HGFF F DLPEYSLMIKEM+DFI
Sbjct: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 333

[Close]

BLAST of HG10003559 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 299.3 bits (765), Expect = 4.2e-81
Identity = 153/320 (47.81%), Postives = 202/320 (63.12%), Query Frame = 0


Query: 39  LPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIHAVSSSDLTIDSSRH 98
           LP K RI L   + + + + R D T+NR  L   D + PPNP  ++ VS+SD  +D SR 
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 99  LFLRILTPTAAADSLSPPLIFYFHGGGFAFGSPDATSMDMAARRFAKELRAVVISVNYRL 158
           L+ R+ TP  + D +  P++ +FHGGGFAF SP+A   D   RRFA++L A VISVNYRL
Sbjct: 73  LWFRLYTPHVSGDKI--PVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRL 132

Query: 159 TPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGESAGGNLGHHVAV---RAS 218
            PE R+P QYDDGFDALK+I+E     L   ADLSRCF  G+SAGGN+ H+VA+   R  
Sbjct: 133 APEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICREP 192

Query: 219 EYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAA 278
              F  VK++G I+ QPFFGGEERTE+E +L     ++ + TDW W+A    G NRDH A
Sbjct: 193 RSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRDHEA 252

Query: 279 ANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKNVKLVVFSKAIHGF 338
            NV GPN  DISG+ ++P T++   G D L D Q+ YYE LK  GK   L+ +    H F
Sbjct: 253 VNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFHAF 312

Query: 339 FGFADLPEYSLMIKEMKDFI 356
           + F +LPE   +I  +KDF+
Sbjct: 313 YIFPELPEAGQLIMRIKDFV 326

[Close]

BLAST of HG10003559 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 1.0e-58
Identity = 135/355 (38.03%), Postives = 192/355 (54.08%), Query Frame = 0


Query: 14  MEGSEEEEGLEMSSSSVCSEISTQQLPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFID 73
           M GSEE   +E          S   +P    +++    L +N   R D T NR L  F+D
Sbjct: 1   MAGSEEVNLIE----------SKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLD 60

Query: 74  PKIPPNPNSIHAVSSSDLTIDSSRHLFLRILTPTAAADSLS------------PPLIFYF 133
            K+P N N ++ V S D+ ID   +L  R+  P  A  S S             P+I +F
Sbjct: 61  RKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFF 120

Query: 134 HGGGFAFGSPDATSMDMAARRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEM 193
           HGG FA  S ++   D   RR      AVV+SVNYR  PE R+PC YDDG+  LK+++  
Sbjct: 121 HGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVN-- 180

Query: 194 DDGALLGRADLS-RCFILGESAGGNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERT 253
               L  + D   R F+ G+S+GGN+ H+VAVRA E    R+ V+G I   P FGG ERT
Sbjct: 181 SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERT 240

Query: 254 ESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGG 313
           ESE RL  +  +T+   DW+WRAFLP GE+R+H A +  GP  + + G+ +FP +++   
Sbjct: 241 ESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVA 300

Query: 314 GLDLLIDRQKCYYEGLKRMGKNVKLVVFSKAIHGFFGFADLPEYSLMIKEMKDFI 356
           GLDL+ D Q  Y EGLK+ G+ VKL+   +A  GF+   +   +  ++ E+  F+
Sbjct: 301 GLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

[Close]

BLAST of HG10003559 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 212.6 bits (540), Expect = 5.2e-55
Identity = 120/332 (36.14%), Postives = 177/332 (53.31%), Query Frame = 0


Query: 39  LPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIHAVSSSDLTIDSSRH 98
           +P    +++    + +N   R D T NR L  ++D K+  N N +  V S D+ ID   +
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 99  LFLRILTPTAAADSLSP--------------PLIFYFHGGGFAFGSPDATSMDMAARRFA 158
           L  R+  P  A     P              P+I +FHGG FA  S ++   D   RR  
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 159 KELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLS-RCFILGESAG 218
              + VV+SVNYR  PE  +PC YDDG+ AL +++      L  + D     F+ G+S+G
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSG 195

Query: 219 GNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRA 278
           GN+ H+VA+RA E     + V+G I   P FGG ERTESE  L  +  +T+   DW+W+A
Sbjct: 196 GNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKA 255

Query: 279 FLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKNV 338
           FLP GE+R+H A N   P G+ + GV +FP +++   GLDL+ D Q  Y EGLK+ G+ V
Sbjct: 256 FLPEGEDREHPACNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEV 315

Query: 339 KLVVFSKAIHGFFGFADLPEYSLMIKEMKDFI 356
           KL+   KA  GF+   +   +  ++ E+  F+
Sbjct: 316 KLMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

[Close]

BLAST of HG10003559 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 208.4 bits (529), Expect = 9.7e-54
Identity = 117/335 (34.93%), Postives = 183/335 (54.63%), Query Frame = 0


Query: 39  LPWKHRIILRFAALLFNTSLRSDLTVNRWLLNFIDPKIPPNPNSIHAVSSSDLTIDSSRH 98
           +P    +++    L +    R D + NR L  F+D K+P N   +  V S D  +DS+ +
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTN 75

Query: 99  LFLRILTPTA--------AADSLSP-------PLIFYFHGGGFAFGSPDATSMDMAARRF 158
           L  RI  P +          +   P       P++ +FHGG F   S ++   D   RR 
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 159 AKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDEMDDGALLGRADLSRCFILGESAG 218
                 VV+SV+YR +PE R+PC YDDG++AL ++ +       G+      ++ G+S+G
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWV-KSRVWLQSGKDSNVYVYLAGDSSG 195

Query: 219 GNLGHHVAVRASEYEFKRVKVVGFIASQPFFGGEERTESENRLCKQLPLTLNMTDWFWRA 278
           GN+ H+VAVRA+    + VKV+G I   P FGG+ERT+SE  L  +  +T+   DW+WRA
Sbjct: 196 GNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRA 255

Query: 279 FLPAGENRDHAAANVNGPNGRDISGVKNFPATVIFGGGLDLLIDRQKCYYEGLKRMGKNV 338
           +LP GE+RDH A N  GP G+ + GV NFP +++   GLDL+ D Q  Y +GLK+ G  V
Sbjct: 256 YLPEGEDRDHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEV 315

Query: 339 KLVVFSKAIHGFFGFADLPEYSLMIKEMKDFIANL 359
            L+   +A  GF+   +   +  +++E+  F+ ++
Sbjct: 316 NLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

[Close]

BLAST of HG10003559 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 169.5 bits (428), Expect = 5.0e-42
Identity = 107/304 (35.20%), Postives = 154/304 (50.66%), Query Frame = 0


Query: 69  LNFIDPKIPPNPNSIHAVSSSDLTIDSSRHLFLRILTPTAAADSLSPPLIFYFHGGGFAF 128
           ++ I  +IP   N    V   D       +L LR+  P +A++  + P++ +FHGGGF F
Sbjct: 32  IDLITQQIPFKNN--QTVLFKDSIYHKPNNLHLRLYKPISASNRTALPVVVFFHGGGFCF 91

Query: 129 GSPDATSMDMAARRFAKELRAVVISVNYRLTPEFRFPCQYDDGFDALKFIDE-------- 188
           GS             A  L A+V+S +YRL PE R P  ++D    L ++ +        
Sbjct: 92  GSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFEDAEAVLTWLWDQAVSDGVN 151

Query: 189 --MDDGALLGRADLSRCFILGESAGGNLGHHVAVR--ASEYEFKRVKVVGFIASQPFFGG 248
              +DG      D  R F++G+S+GGN+ H +AVR  +   E   V+V G++   PFFGG
Sbjct: 152 HWFEDGT---DVDFDRVFVVGDSSGGNIAHQLAVRFGSGSIELTPVRVRGYVLMGPFFGG 211

Query: 249 EERTESENRLCKQLPLTLNMTDWFWRAFLPAGENRDHAAANVNGPNGRDISGVKNFPATV 308
           EERT SEN   + L L+L++ D FWR  LP G  RDH  AN  GP    +  +   P  V
Sbjct: 212 EERTNSENGPSEAL-LSLDLLDKFWRLSLPNGATRDHHMANPFGPTSPTLESISLEPMLV 271

Query: 309 IFGGGLDLLIDRQKCYYEGLKRM-GKNVKLVVFSKAIHGFF-GFADLPEYSLMIKEMKDF 359
           I GG  +LL DR K Y   LK+M GK V  + F    HGF+  +        +++ + DF
Sbjct: 272 IVGGS-ELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFYSNYPSSEAAEQVLRIIGDF 328

The following BLAST results are available for this feature:

BLAST of HG10003559 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141618.1	2.9e-161	84.02	probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein ...	[more]
XP_008459713.1	5.0e-161	84.23	PREDICTED: probable carboxylesterase 18 [Cucumis melo]	[more]
KAA0039340.1	6.1e-159	83.33	putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00523.1 putative car...	[more]
XP_022925491.1	1.8e-158	83.23	probable carboxylesterase 18 [Cucurbita moschata]	[more]
XP_022973499.1	1.2e-157	83.18	probable carboxylesterase 18 [Cucurbita maxima]	[more]

BLAST of HG10003559 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LT10	5.9e-80	47.81	Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1	[more]
Q940G6	1.4e-57	38.03	Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1	[more]
Q6L545	2.7e-56	40.12	Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ...	[more]
Q9MAA7	7.3e-54	36.14	Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1	[more]
Q9LYC1	1.4e-52	34.93	Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1	[more]

BLAST of HG10003559 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXS8	1.4e-161	84.02	Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1	[more]
A0A1S3CBA4	2.4e-161	84.23	probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1	[more]
A0A5D3BL91	3.0e-159	83.33	Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A6J1ECC8	8.6e-159	83.23	probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 ...	[more]
A0A6J1IER7	5.6e-158	83.18	probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV...	[more]

BLAST of HG10003559 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23530.1	4.2e-81	47.81	carboxyesterase 18	[more]
AT5G27320.1	1.0e-58	38.03	alpha/beta-Hydrolases superfamily protein	[more]
AT3G05120.1	5.2e-55	36.14	alpha/beta-Hydrolases superfamily protein	[more]
AT3G63010.1	9.7e-54	34.93	alpha/beta-Hydrolases superfamily protein	[more]
AT5G06570.1	5.0e-42	35.20	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 118..336 e-value: 1.4E-51 score: 175.4
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 21..356 e-value: 1.9E-83 score: 282.6
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 69..356
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..17
None	No IPR available	PANTHER	PTHR23024:SF434	ACETYL ESTERASE	coord: 34..356
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 34..356
IPR002168	Lipase, GDXG, putative histidine active site	PROSITE	PS01173	LIPASE_GDXG_HIS	coord: 118..134

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10003559.1	HG10003559.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity