HG10003521 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10003521 vs. NCBI nr
Match: XP_038890436.1 (LOW QUALITY PROTEIN: TMV resistance protein N-like [Benincasa hispida])

HSP 1 Score: 825.1 bits (2130), Expect = 5.3e-235
Identity = 520/1108 (46.93%), Postives = 599/1108 (54.06%), Query Frame = 0

Query: 95   KYEYEKSVSRFIYSVLNKKNRSSVVEELQGWRDAIKKLRSLSKVSVPIHFNVYKTEEITK 154
            KY + +++  FI +    KN     +E+ GWRDA+ ++ SLS V +     +  T +I +
Sbjct: 120  KYFFGQALRDFIRNGPKNKN----WKEVYGWRDAMSQICSLSGVVLQPLNLLSDTRKIRR 179

Query: 155  QIFDKLLHLKLVAQNKYLLEMPLRLNRMKMFFGLSLSS--IRFIGIVGMGGIGKTTIAEA 214
             IFDKLL+LKL A+N YL EM  RL  M+M  G+       R IGIVGMGGIGKTTIA+ 
Sbjct: 180  MIFDKLLNLKLEAKNSYLFEMEHRLRTMEMLLGIGSDEEPARLIGIVGMGGIGKTTIAKL 239

Query: 215  LYDKFAHKFTNS-CFVRIAGHNLVSLQQQLLSQL-LTKDIKISDENHGLRMIIDYLTSHK 274
            LYDKFA  F+N+ CF+ I+G N+VSLQQQLLSQL   +DIKI DEN G+  I   L S +
Sbjct: 240  LYDKFATTFSNNCCFLHISGSNIVSLQQQLLSQLFFLQDIKICDENVGVERIKHNLRSCQ 299

Query: 275  KVFIVFDGIIENRQLEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSD 334
            KV +VFDGI E  QLEML G+P+WF ++SRIIVTTRNK ILRQ N++ KV EYNVELLS 
Sbjct: 300  KVLLVFDGITEKSQLEMLAGSPDWFPAKSRIIVTTRNKHILRQINFKYKVQEYNVELLSH 359

Query: 335  TSASSLFCKHAF-GDDPPNENLKDLCNEIIEKIGRHPLALVKIASSLYGQ---------- 394
            TSA SLFCKHAF    PP++N ++L NEIIEK+G  PLA++KIASSLYGQ          
Sbjct: 360  TSAFSLFCKHAFRRKHPPDDNFQELSNEIIEKVGTLPLAVIKIASSLYGQGIDVWEDKLK 419

Query: 395  ------------------------------------------------------------ 454
                                                                        
Sbjct: 420  GYHKLVFDNIFSDVLKSSYEGLEAESQQIFLDLACFLNGEKVDRVTEILQGFGYNSPDAQ 479

Query: 455  -------------DEQIQMHILNIYMGQEIVRREMGTHRQSRIWLREDVRRLFDENYELK 514
                         +E IQ+HIL + M QE+VRR++GTH+Q+RIWLRED  R+F ENY+LK
Sbjct: 480  LQLLADTCLIDISNEHIQIHILILCMAQELVRRKLGTHQQTRIWLREDASRVFHENYDLK 539

Query: 515  YIQGIVMDLEEEEELVLEAKSLTDMSELKILQINNVRLNEDIECLSNKLTLLNWPGYPSK 574
             IQGI+MDLEEEEELVLEAKS  DM+ELKILQINNV+++EDIECLSNKLTLLNWPGYPSK
Sbjct: 540  CIQGIMMDLEEEEELVLEAKSFADMTELKILQINNVQISEDIECLSNKLTLLNWPGYPSK 599

Query: 575  NLPSTFQPPPLLQLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRL 634
             LPSTFQP PLL+LHLPGSNVERLWNG KKF NLKEID S S+YL+ETPDFSEVRNLRRL
Sbjct: 600  YLPSTFQPLPLLELHLPGSNVERLWNGTKKFGNLKEIDASDSKYLVETPDFSEVRNLRRL 659

Query: 635  ILRNCGRLHKVHSSINRLERLVLLDMEGCVSFT--------------------------- 694
            ILRNCGRL +VHSSIN L+RLVLLDMEGCV FT                           
Sbjct: 660  ILRNCGRLQEVHSSINSLKRLVLLDMEGCVHFTRFLFPITCKRLKTLRLSNSGLEFFPEF 719

Query: 695  ------------------------------------------SLPVEMSSLSCLKTLILN 754
                                                      SL  E+ SLS LKTLILN
Sbjct: 720  GCCMEYLIELHIDGTSINQVSPSITYLIALVLLNLKNCIRLSSLSTEIGSLSSLKTLILN 779

Query: 755  GCKDLDQIPPSLGNVEPLEELDIGGTSISVIPFLKNLRILNCERLESNIWHSLAG-STHC 814
            GCK+LDQIPPSLGN++PLEELD+GGTSISVIPFL+NLRILNCERL+SNIWHSLA   T  
Sbjct: 780  GCKNLDQIPPSLGNIKPLEELDVGGTSISVIPFLENLRILNCERLKSNIWHSLASLPTQS 839

BLAST of HG10003521 vs. NCBI nr
Match: KAA0039329.1 (TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 788.1 bits (2034), Expect = 7.2e-224
Identity = 502/1091 (46.01%), Postives = 573/1091 (52.52%), Query Frame = 0

Query: 120  EELQGWRDAIKKLRSLSKVSVPIHFNVYKTEEITKQIFDKLLHLKLVAQNKYLLEMPLRL 179
            EELQ WR+AI ++ SLS V++             K I+DKLLHLK VA+++YL EMPLRL
Sbjct: 160  EELQRWREAISQICSLSGVAL----RPTSLMRTIKLIYDKLLHLKSVAEDRYLFEMPLRL 219

Query: 180  NRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNS-CFVRIAGHNLVSLQ 239
              M+M  G     +RFIGIVGMGGIGKTTIA+ +Y KFA KF N+ CF+ IAG N+VSLQ
Sbjct: 220  RTMEMLLGSISDDVRFIGIVGMGGIGKTTIAQFVYQKFAPKFRNNCCFLHIAGSNIVSLQ 279

Query: 240  QQLLSQL-LTKDIKISDENHGLRMIIDYLTSHKKVFIVFDGIIENRQLEMLVGNPNWFSS 299
            QQLLSQ+   +DIKI DE  G+  I   L S +KV  +FDGI +  QLEML GNP+W  +
Sbjct: 280  QQLLSQVFFLEDIKIVDEILGVNRIKHNLKSCQKVLFIFDGISKKSQLEMLAGNPDWLPA 339

Query: 300  RSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGDDPPNENLKDLCNE 359
             SRII+TTRNKDILRQ+N++DKV EY+ ELLS TSA SLFCK+AFG+  P+EN K+L NE
Sbjct: 340  GSRIIITTRNKDILRQTNFKDKVQEYSAELLSHTSAVSLFCKYAFGECLPDENFKELSNE 399

Query: 360  IIEKIGRHPLALVKIASSLYGQ-------------------------------------- 419
            IIEK G  PLALV+IASSLYGQ                                      
Sbjct: 400  IIEKTGTLPLALVQIASSLYGQGIDVWEDTLKSFHKLVYDNIFSHVLKSSYEGLQAESQQ 459

Query: 420  ---------------------------------------------DEQIQMHILNIYMGQ 479
                                                         D+QIQMH+L + MGQ
Sbjct: 460  IFLDLACFLNGEKVDRVVEILEGFGYNSPRTKLQLLADRYLIDLSDDQIQMHVLILCMGQ 519

Query: 480  EIVRREMGTHRQSRIWLREDVRRLFDENYELKYIQGIVMDL-EEEEELVLEAKSLTDMSE 539
            EIV+RE+GTH+Q+RIW RED RRLF ENY LKYIQGI MDL EEEEELVLEAKSL DM E
Sbjct: 520  EIVQRELGTHQQTRIWQREDARRLFHENYGLKYIQGITMDLGEEEEELVLEAKSLADMIE 579

Query: 540  LKILQINNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQLHLPGSNVERLWNG 599
            LKILQINNV+++E+I+CLSNKLTLLNWPGYPSK LPSTFQPPPLL+L LPGSNV RLWNG
Sbjct: 580  LKILQINNVQISENIDCLSNKLTLLNWPGYPSKYLPSTFQPPPLLELRLPGSNVIRLWNG 639

Query: 600  RKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKVHSSINRLERLVLLDME 659
            RKKF NLKEID S S++L+ETPD SEV NL+RLIL+NC  L +VH+SIN LERLVLLDME
Sbjct: 640  RKKFGNLKEIDASDSKHLVETPDLSEVPNLQRLILQNCETLQRVHASINSLERLVLLDME 699

Query: 660  GCVS-------------------------------------------------------- 719
            GCVS                                                        
Sbjct: 700  GCVSLKRFSFPITCKRLKTLVLSYSGLEFFPEFGWWMEYLTELHIDGTSINQLSPSITYL 759

Query: 720  -------------FTSLPVEMSSLSCLKTLILNGCKDLDQIPPSLGNVEPLEELDIGGTS 779
                          +SLP E+  L  LKTL LNGC+ LD+IP SLG VEPLEELDIGGTS
Sbjct: 760  TSLVLLNLRNCIGLSSLPTEICCLCSLKTLTLNGCESLDKIPSSLGYVEPLEELDIGGTS 819

Query: 780  ISVIPFLKNLRILNCERLESNIWHSLAG-STHCFRSLKDLNLSDCNLANEDIPDDLDLFS 810
            IS IPFL+NLRILNCERL+SNIWHSLAG   H  RSLKDLNLSDCNL +EDIP+DL+LFS
Sbjct: 820  ISTIPFLENLRILNCERLKSNIWHSLAGLPAHYVRSLKDLNLSDCNLVDEDIPNDLELFS 879

BLAST of HG10003521 vs. NCBI nr
Match: XP_031741454.1 (TMV resistance protein N [Cucumis sativus])

HSP 1 Score: 776.2 bits (2003), Expect = 2.8e-220
Identity = 487/1098 (44.35%), Postives = 585/1098 (53.28%), Query Frame = 0

Query: 64   RSFISSSDNEDTGLVQITRASGDIGK-GDKKSKYEYEKSVSRFIYSVLNKKNRSSVVEEL 123
            RS  S S N +T       A  ++G    ++ K+ Y+ +  +FI    N + +   ++E+
Sbjct: 125  RSLRSLSRNRETD------AYSEVGSMTSRQVKFRYQHAFEKFI----NSEAKKDYLKEV 184

Query: 124  QGWRDAIKKLRSLSKVSVP--IHFNVYKTEEITKQIFDKLLHLKLVAQNKYLLEMPLRLN 183
              W  ++ ++  L  V +P  I +  +K + I   I D LL LKL A+ + L EMPLRL 
Sbjct: 185  DKWWLSVLEVSDLPGVDIPQRISYTKFKIQSIANSIGDHLLRLKLQAKEENLFEMPLRLR 244

Query: 184  RMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNSC----FVRIAGHNLVS 243
             MKM  GL  + +RFIGIVGM GIGKTT+AE  Y +    F ++     F+   G ++VS
Sbjct: 245  TMKMLLGLGSNDVRFIGIVGMSGIGKTTLAEMTYLRIFKPFVSALRKPYFLHFVGRSIVS 304

Query: 244  LQQQLLSQLL---TKDIKISDENHGLRMIIDYLTSHKKVFIVFDGIIENRQLEMLVGNPN 303
            LQQQLL QL      DI++ DENHG+ +I+ +L+S K V IVFDGI E  QLEML G+P+
Sbjct: 305  LQQQLLDQLAFLKPIDIQVLDENHGVELIMQHLSSLKNVLIVFDGITERSQLEMLAGSPD 364

Query: 304  WFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGDDPPNENLKD 363
            WF + SRII+TT NK+I    N++DKV EYNVELLS  +A SLFCK AFGD P  +N+ D
Sbjct: 365  WFGAGSRIIITTTNKNIFHHPNFKDKVQEYNVELLSHEAAFSLFCKLAFGDHPHTQNMDD 424

Query: 364  LCNEIIEKIGRHPLALVKIASSLYGQ---------------------------------- 423
            LCNE+IEK+GR PLAL KIA SLYGQ                                  
Sbjct: 425  LCNEMIEKVGRLPLALEKIAFSLYGQNIDVWEHTLKNFHQVVYDNIFSDVLKSSYEGLEA 484

Query: 424  -------------------------------------------------DEQIQMHILNI 483
                                                             D  IQMHIL +
Sbjct: 485  ESQQIFLDLACFLNGEKVDRVIQILQGFGYTSPQTNLQLLVDRCLIDILDGHIQMHILIL 544

Query: 484  YMGQEIVRREMGTHRQSRIWLREDVRRLFDENYELKYIQGIVMDLEEEEELVLEAKSLTD 543
             MGQEIV RE+G  +Q+RIWLR+D RRLF EN ELKYI+GIVMDLEEEEELVL+AK+  D
Sbjct: 545  CMGQEIVHRELGNCQQTRIWLRDDARRLFHENNELKYIRGIVMDLEEEEELVLKAKAFAD 604

Query: 544  MSELKILQINNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQLHLPGSNVERL 603
            MSEL+IL+INNV+L+EDIECLSNKLTLLNWPGYPSK LPSTFQPP LL+LHLPGSNVERL
Sbjct: 605  MSELRILRINNVQLSEDIECLSNKLTLLNWPGYPSKYLPSTFQPPSLLELHLPGSNVERL 664

Query: 604  WNGRKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKVHSSINRLERLVLL 663
            WNG + FKNLKEID S S++L+ETP+FSE   LRRLILRNCGRL+KVHSSIN L RL+LL
Sbjct: 665  WNGTQNFKNLKEIDASDSKFLVETPNFSEAPKLRRLILRNCGRLNKVHSSINSLHRLILL 724

Query: 664  DMEGCVSF---------------------------------------------------- 723
            DMEGCVSF                                                    
Sbjct: 725  DMEGCVSFRSFSFPVTCKSLKTLVLSNCGLEFFPEFGCVMGYLTELHIDGTSINKLSPSI 784

Query: 724  -----------------TSLPVEMSSLSCLKTLILNGCKDLDQIPPSLGNVEPLEELDIG 783
                             +SLP E+  LS LKTLILNGCK+LD+IPP L  V+ LEELDIG
Sbjct: 785  TNLLGLVLLNLRNCIRLSSLPTEICRLSSLKTLILNGCKNLDKIPPCLRYVKHLEELDIG 844

Query: 784  GTSISVIPFLKNLRILNCERLESNIWHSLAG-STHCFRSLKDLNLSDCNLANEDIPDDLD 812
            GTSIS IPFL+NLRILNCERL+SNIWHSLAG +    RSL DLNLSDCNL +EDIP+DL+
Sbjct: 845  GTSISTIPFLENLRILNCERLKSNIWHSLAGLAAQYLRSLNDLNLSDCNLVDEDIPNDLE 904

BLAST of HG10003521 vs. NCBI nr
Match: QOL20471.1 (resistance gene-like protein [Cucumis melo])

HSP 1 Score: 760.0 bits (1961), Expect = 2.1e-215
Identity = 479/1050 (45.62%), Postives = 548/1050 (52.19%), Query Frame = 0

Query: 146  VYKTEEITKQIFDKLLHLKLVAQNKYLLEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIG 205
            +++ +   KQI D+LL LKL A+   L EMPLRL  M+M  GL  + IRFIGIVGM GIG
Sbjct: 209  MFQIKSTAKQIIDRLLSLKLEAKEGNLFEMPLRLRTMEMLLGLGSNDIRFIGIVGMSGIG 268

Query: 206  KTTIAEALYDKFAHKFTN-------SCFVRIAGHNLVSLQQQLLSQLLTK---DIKISDE 265
            KTT+AE +Y   AH F +        CF+   G ++VSLQQQLL QL +    DI+I DE
Sbjct: 269  KTTLAEVIY---AHSFKSLISDLGKRCFLHSRGRSIVSLQQQLLDQLASSKMIDIQILDE 328

Query: 266  NHGLRMIIDYLTSHKKVFIVFDGIIENRQLEMLVGNPNWFSSRSRIIVTTRNKDILRQSN 325
            NHG+R+I  +L   KKV IV DGI E  QLEML G+P+WF   SRII+TT NKDI R  N
Sbjct: 329  NHGVRLIKQHLRFLKKVLIVLDGISETSQLEMLAGSPDWFGKGSRIIITTTNKDIFRHPN 388

Query: 326  YQDKVHEYNVELLSDTSASSLFCKHAFGDDPPNENLKDLCNEIIEKIGRHPLALVKIASS 385
            ++DKV EYNVEL S  +A SLFCK AFGD PP+E++KDLCNEIIEK+GR PLAL KIASS
Sbjct: 389  FKDKVQEYNVELPSHEAAFSLFCKLAFGDYPPSEDMKDLCNEIIEKVGRLPLALEKIASS 448

Query: 386  LYGQ-------------------------------------------------------- 445
            LYG                                                         
Sbjct: 449  LYGHDMNIWEDTLKNFHKVVYDNIFSDILKSSYEGLEAESQQIFLDLACFLNGEKVDRVI 508

Query: 446  ---------------------------DEQIQMHILNIYMGQEIVRREMGTHRQSRIWLR 505
                                       D  IQMHIL + MG+EIVRR++GT +Q+RIWLR
Sbjct: 509  EILQGFGYSSPQTNLQLLVDRCLIDILDGHIQMHILILCMGKEIVRRKLGTCQQTRIWLR 568

Query: 506  EDVRRLFDENYELKYIQGIVMDLEEEEELVLEAKSLTDMSELKILQINNVRLNEDIECLS 565
            +D RRLF EN ELKYI GIVMDLEEEEELVL+AK+   MSELKIL+INNV+L+EDIE LS
Sbjct: 569  DDARRLFHENNELKYICGIVMDLEEEEELVLKAKAFAGMSELKILRINNVQLSEDIEFLS 628

Query: 566  NKLTLLNWPGYPSKNLPSTFQPPPLLQLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLI 625
            NKLTLLNWPGYPSK LPSTFQPP L++LHLPGSNVERLWNG +KFKNLKEID S S+YL+
Sbjct: 629  NKLTLLNWPGYPSKYLPSTFQPPSLIELHLPGSNVERLWNGTQKFKNLKEIDASDSKYLV 688

Query: 626  ETPDFSEVRNLRRLILRNCGRLHKVHSSINRLERLVLLDMEGCVSF-------------- 685
            ETP+FSE RNLRRLILRNCGRL +VHSSIN L RL+L DMEGCVSF              
Sbjct: 689  ETPNFSEARNLRRLILRNCGRLKEVHSSINSLHRLILFDMEGCVSFKSFSFVITCESLKT 748

Query: 686  -------------------------------------------------------TSLPV 745
                                                                   +SLP 
Sbjct: 749  LVLSNCGLEFFPEFGFPMGYLTELHIDGTSINELSPSIKNLLGLVLLNLGNCIRLSSLPT 808

Query: 746  EMSSLSCLKTLILNGCKDLDQIPPSLGNVEPLEELDIGGTSISVIPFLKNLRILNCERLE 805
            E+ SLS LKTLILNGCK+L ++PPSL  V+PLEELDIGGTSIS IPF++NLRILNCERL+
Sbjct: 809  EIGSLSSLKTLILNGCKNLHKLPPSLEYVKPLEELDIGGTSISTIPFVENLRILNCERLK 868

Query: 806  SNIWHSLAG-STHCFRSLKDLNLSDCNLANEDIPDDLDLFSSLEILDLSNNHFESLPESM 810
            S IWHSLA   T  F SLKDLNLSDCNL +EDIP DL+LFSSLEILDL +NHFE L ES+
Sbjct: 869  SIIWHSLASLPTEYFSSLKDLNLSDCNLVDEDIPSDLELFSSLEILDLGSNHFERLSESI 928

BLAST of HG10003521 vs. NCBI nr
Match: AGH33854.2 (resistance gene-like protein [Cucumis melo])

HSP 1 Score: 757.7 bits (1955), Expect = 1.0e-214
Identity = 489/1104 (44.29%), Postives = 573/1104 (51.90%), Query Frame = 0

Query: 92   KKSKYEYEKSVSRFIYSVLNKKNRSSVVEELQGWRDAIKKLRSLSKVSVPIHFNVYKTEE 151
            ++S  ++ KS++   YS +N +   ++ E  +     I +  S +K         ++ + 
Sbjct: 163  RQSHRKFFKSMAEKDYSEVNYRTSLAMSEFCRLPGIYISRKNSNTK---------FQIKS 222

Query: 152  ITKQIFDKLLHLKLVAQNKYLLEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAE 211
              KQI D+LL LKL A+   L EMPLRL  M+M  GL  + IRFIGIVGM GIGKTT+AE
Sbjct: 223  TAKQIIDRLLSLKLEAKEGNLFEMPLRLRTMEMLLGLGSNDIRFIGIVGMSGIGKTTLAE 282

Query: 212  ALYDKFAHKFTN-------SCFVRIAGHNLVSLQQQLLSQLLTK---DIKISDENHGLRM 271
             +Y   AH F +        CF+   G ++VSLQQQLL QL +    DI+I DENHG+R+
Sbjct: 283  MMY---AHSFKSLISGLRKRCFLHSRGRSIVSLQQQLLDQLASSKMIDIQILDENHGVRL 342

Query: 272  IIDYLTSHKKVFIVFDGIIENRQLEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVH 331
            I  +L+S  KV IVFDGI E  QLEML G+P+WF + SRII+TT NK I R  N++DKV 
Sbjct: 343  IKQHLSSLIKVLIVFDGISETSQLEMLAGSPDWFGAGSRIIITTTNKYIFRHPNFKDKVQ 402

Query: 332  EYNVELLSDTSASSLFCKHAFGDDPPNENLKDLCNEIIEKIGRHPLALVKIASSLYGQ-- 391
            EYNVELLS  +A SLFCK AFGD PP+E++KDLCNEIIEK+GR PLAL KIASSLYG   
Sbjct: 403  EYNVELLSHEAAFSLFCKLAFGDYPPSEDMKDLCNEIIEKVGRLPLALEKIASSLYGHDM 462

Query: 392  ------------------------------------------------------------ 451
                                                                        
Sbjct: 463  DIWEDTLKNFHKVVYDNIFSDILKSSYEGLEAESQQIFLDLACFLNGEKVDRVIEILQGF 522

Query: 452  ---------------------DEQIQMHILNIYMGQEIVRREMGTHRQSRIWLREDVRRL 511
                                 D  IQMHIL + MG+EIVRR++GT +Q+RIWLR+D RRL
Sbjct: 523  GYSSPQTNLQLLVDRCLIDILDGHIQMHILILCMGKEIVRRKLGTCQQTRIWLRDDARRL 582

Query: 512  FDENYELKYIQGIVMDLEEEEELVLEAKSLTDMSELKILQINNVRLNEDIECLSNKLTLL 571
            F EN ELKYI GIVMDLEEEEELVL+AK+   MSELKIL+INNV+L+EDIE LSNKLTLL
Sbjct: 583  FHENNELKYICGIVMDLEEEEELVLKAKAFAGMSELKILRINNVQLSEDIEFLSNKLTLL 642

Query: 572  NWPGYPSKNLPSTFQPPPLLQLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFS 631
            NWPGYPSK LPSTFQPP L++LHLPGSNVERLWNG +KFKNLKEID S S+YL+ETP+FS
Sbjct: 643  NWPGYPSKYLPSTFQPPSLIELHLPGSNVERLWNGTQKFKNLKEIDASDSKYLVETPNFS 702

Query: 632  EVRNLRRLILRNCGRLHKVHSSINRLERLVLLDMEGCVSF-------------------- 691
            E RNLRRLILRNCGRL +VHSSIN L RL+L DMEGCVSF                    
Sbjct: 703  EARNLRRLILRNCGRLKEVHSSINSLHRLILFDMEGCVSFKSFSFVITCESLKTLVLSNC 762

Query: 692  -------------------------------------------------TSLPVEMSSLS 751
                                                             +SLP E+ SLS
Sbjct: 763  GLEFFPEFGFPMGYLTELHIDGTSINELSPSIKNLLGLVLLNLGNCIRLSSLPTEIGSLS 822

Query: 752  CLKTLILNGCKDLDQIPPSLGNVEPLEELDIGGTSISVIPFLKNLRILNCERLESNIWHS 810
             LKTLILNGCK+L ++PPSL  V+PLEELDIGGTSIS IPF++NLRILNCERL+S IWHS
Sbjct: 823  SLKTLILNGCKNLHKLPPSLEYVKPLEELDIGGTSISTIPFVENLRILNCERLKSIIWHS 882

BLAST of HG10003521 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 5.1e-71
Identity = 217/778 (27.89%), Postives = 364/778 (46.79%), Query Frame = 0

Query: 92  KKSKYEYEKSVSRFIYSVLNKKNRSSVVEELQGWRDAIKKLRSLSKVSVPIHFNVYKT-- 151
           +K   EY    ++F  ++++ +      +++  WR+A+ K+ ++S   +   +N  ++  
Sbjct: 114 RKQNGEYAVCFTKFEANLVDDR------DKVLRWREALTKVANISGHDLRNTYNGDESKC 173

Query: 152 -EEITKQIFDKLLHLKLVAQNKYLLEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTT 211
            ++I K IFDK     +   N+ L+ +  ++ ++     + L  +R +GI GMGG+GKTT
Sbjct: 174 IQQILKDIFDKFC-FSISITNRDLVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGKTT 233

Query: 212 IAEALYDKFAHKFTNSCFVR-----IAGHNLVSLQQQLLSQLLTKDIKISDENHGLRMII 271
            A AL++++   F ++CF+      +  H L+ LQ+ LLS+LL  +     +   + +I+
Sbjct: 234 AARALFNRYYQNFESACFLEDVKEYLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVIL 293

Query: 272 DYLTSHKKVFIVFDGIIENRQLEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHE- 331
                 KKV +V D +  N QL+ LVG  +WF S SRI++TTR+  +L+  +    VHE 
Sbjct: 294 KRRLCSKKVLVVLDDVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNHD----VHET 353

Query: 332 YNVELLSDTSASSLFCKHAFGDDPPNENLKDLCNEIIEKIGRHPLALVKIASSLYGQD-- 391
           Y +++L    A  LF  HAF    P +  K+L N +++  G  PLAL  + S LY +D  
Sbjct: 354 YEIKVLEKDEAIELFNLHAFKRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLD 413

Query: 392 ------------------------------------------------------------ 451
                                                                       
Sbjct: 414 VWISTIDRLKDNPEGEIMATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGF 473

Query: 452 -------------------EQIQMHILNIYMGQEIVRREMGTHRQSRIWLREDVRRLFDE 511
                              ++IQMH L   MG++I  +E       RI+  EDV+     
Sbjct: 474 HPVLGVKTLVEKSLIFILEDKIQMHDLMQEMGRQIAVQE---SPMRRIYRPEDVKDACIG 533

Query: 512 NYELKYIQGIVMDLEE-----EEELVLEAKSLTDMSELKIL--QINNVRLNEDIECLSNK 571
           +   + I+G+++   E     E E +  A++L     L+IL  +  N   +E +  L N 
Sbjct: 534 DMRKEAIEGLLLTEPEQFEEGELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAYLPNS 593

Query: 572 LTLLNWPGYPSKNLPSTFQPPPLLQLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLIET 631
           L  L W  Y S + PS F+P  L+ L + GS++  LWNG K+   L  +D+S    LI+T
Sbjct: 594 LLWLEWRNYSSNSFPSNFEPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQT 653

Query: 632 PDFSEVRNLRRLILRNCGRLHKVHSSINRLERLVLLDMEGCVSFTSLPVEMSSLSCLKTL 691
           PDF  + NL RLIL +C  L +VH S+  L+ L+LL+M+ C+S   LP  + S  CL+ L
Sbjct: 654 PDFRMITNLERLILSSCDALVEVHPSVGFLKNLILLNMDHCISLERLPAIIQS-ECLEVL 713

Query: 692 ILNGCKDLDQIPPSLGNVEPLEELDIGGTSISVIP-------FLKNLRILNCER---LES 746
            LN C +L   P    N+  L++LD+  T I  +P        L+NL++ +C +   L S
Sbjct: 714 DLNYCFNLKMFPEVERNMTHLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPS 773

BLAST of HG10003521 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 5.3e-60
Identity = 211/758 (27.84%), Postives = 328/758 (43.27%), Query Frame = 0

Query: 120 EELQGWRDAIKKLRSLSKVS-VPIHFNVYKTEEITKQIFDKL------LHLKLVAQNKYL 179
           +++  WR A+ +  +LS    +   +   + +EIT  IF +L      +   LV  + ++
Sbjct: 144 DKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQLKCKRLDVGANLVGIDSHV 203

Query: 180 LEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNSCFVR--IA 239
            EM LRL+       L  S +R +GI G+GGIGKTTIA+ +Y++ + +F    F+     
Sbjct: 204 KEMILRLH-------LESSDVRMVGIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIRE 263

Query: 240 GHN---LVSLQQQLLSQLLTKD--IKISDENHGLRMIIDYLTSHKKVFIVFDGIIENRQL 299
           G N   L  LQ QLL  +L  +    IS   H   MI D L S ++VFIV D + +  QL
Sbjct: 264 GSNPQVLFHLQNQLLGDILEGEGSQNISSVAHRASMIKDILLS-RRVFIVLDDVDDLSQL 323

Query: 300 EMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGDD 359
           E L+G+  W    SR+I+TTRNK +L      D    Y VE L+   A  LF  +AF  +
Sbjct: 324 EYLLGHREWLGEGSRVIITTRNKHVLAVQEVDDL---YEVEGLNFEEACELFSLYAFKQN 383

Query: 360 PPNENLKDLCNEIIEKIGRHPLALVKIASSL----------------------------- 419
            P  + ++L   ++      PLAL  + S L                             
Sbjct: 384 LPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLDSEPKADIHKVLKR 443

Query: 420 ----------------------YGQD------------------------------EQIQ 479
                                  G+D                               QI 
Sbjct: 444 SYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDFPAETGISNLNDLCLITLPYNQIC 503

Query: 480 MHILNIYMGQEIVRRE--MGTHRQSRIWLREDVRRLFDENYELKYIQGIVMDLEEEEELV 539
           MH L   MG EIVR    +  ++ SR+W   D  R    +  +K ++ + +DL + + + 
Sbjct: 504 MHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGIKSVETMSLDLSKLKRVC 563

Query: 540 LEAKSLTDMSELKILQI----------------------------NNVRLNEDIECLSNK 599
             +     M++L++L++                            + ++L +  +  S +
Sbjct: 564 SNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPSYE 623

Query: 600 LTLLNWPGYPSKNLPSTFQPPPLLQLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLIET 659
           L  L W GYP  +LP  F    L++LHL  SN+++LW G K  + LK ID+S S  L + 
Sbjct: 624 LRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLWQGHKDLERLKVIDLSYSRKLSQM 683

Query: 660 PDFSEVRNLRRLILRNCGRLHKVHSSINRLERLVLLDMEGCVSFTSLPVEMSSLSCLKTL 719
            +FS + NL RL L  C  L  +H S+  +++L  L +  C    +LP  +  L  L++L
Sbjct: 684 SEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRSCNKLKNLPDSIGDLESLESL 743

Query: 720 ILNGCKDLDQIPPSLGNVEPLEELDIGGTSISVIP-------FLKNLRILNCER------ 728
            L+ C   ++ P   GN++ L ELD+  T+I  +P        L++L + NC +      
Sbjct: 744 YLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLESLYLSNCSKFEKFPE 803

BLAST of HG10003521 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 4.9e-58
Identity = 212/782 (27.11%), Postives = 335/782 (42.84%), Query Frame = 0

Query: 119 VEELQGWRDAIKKLRSLSKVSVPIHFNVYKTE-----EITKQIFDKLLHLKLVAQNKYLL 178
           VE +Q WR A+ +  +L K S     N  KT+     +I  QI  KL  + L +  + ++
Sbjct: 135 VEGIQRWRIALNEAANL-KGSCD---NRDKTDADCIRQIVDQISSKLCKISL-SYLQNIV 194

Query: 179 EMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKF------AHKFTNSCFV 238
            +   L +++    + ++ +R +GI GMGG+GKTTIA A++D        +++F  +CF+
Sbjct: 195 GIDTHLEKIESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFL 254

Query: 239 RIAGHN---LVSLQQQLLSQLLTKDIKISDENHGLRMIIDYLTSHKKVFIVFDGI-IENR 298
           +    N   + SLQ  LLS+LL +    ++E  G   +   L S KKV IV D I  ++ 
Sbjct: 255 KDIKENKRGMHSLQNALLSELLREKANYNNEEDGKHQMASRLRS-KKVLIVLDDIDNKDH 314

Query: 299 QLEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFG 358
            LE L G+ +WF + SRII+TTR+K ++ +++       Y V  L D  +  LF +HAFG
Sbjct: 315 YLEYLAGDLDWFGNGSRIIITTRDKHLIEKNDI-----IYEVTALPDHESIQLFKQHAFG 374

Query: 359 DDPPNENLKDLCNEIIEKIGRHPLAL---------------------------------- 418
            + PNEN + L  E++      PLAL                                  
Sbjct: 375 KEVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIIDKL 434

Query: 419 ---------------VKIASSLYGQDE--------------------------------- 478
                          + IA  L G+++                                 
Sbjct: 435 KISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRILIDKSLVFISEYN 494

Query: 479 QIQMHILNIYMGQEIVRREMGTHRQSRIWLREDVRRLFDENYELKYIQGIVMDLEEEEEL 538
           Q+QMH L   MG+ IV  +     +SR+WL ++V  +   N     ++ I +       L
Sbjct: 495 QVQMHDLIQDMGKYIVNFQKDPGERSRLWLAKEVEEVMSNNTGTMAMEAIWVS-SYSSTL 554

Query: 539 VLEAKSLTDMSELKILQINNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQLH 598
               +++ +M  L++  +     +  I+ L N L       YP ++ PSTF+   L+ L 
Sbjct: 555 RFSNQAVKNMKRLRVFNMGRSSTHYAIDYLPNNLRCFVCTNYPWESFPSTFELKMLVHLQ 614

Query: 599 LPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKVHSSI 658
           L  +++  LW   K   +L+ ID+S S+ L  TPDF+ + NL  + L  C  L +VH S+
Sbjct: 615 LRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSL 674

Query: 659 N----------------------RLERLVLLDMEGCVSFTSLP-----------VEM--- 718
                                   +E L  L +  C S   LP           + M   
Sbjct: 675 GCCSKVIGLYLNDCKSLKRFPCVNVESLEYLGLRSCDSLEKLPEIYGRMKPEIQIHMQGS 734

Query: 719 -------------------------------SSLSCLKTLI---LNGCKDLDQIPPSLGN 730
                                          SS+  LK+L+   ++GC  L+ +P  +G+
Sbjct: 735 GIRELPSSIFQYKTHVTKLLLWNMKNLVALPSSICRLKSLVSLSVSGCSKLESLPEEIGD 794

BLAST of HG10003521 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 6.0e-56
Identity = 196/727 (26.96%), Postives = 311/727 (42.78%), Query Frame = 0

Query: 120 EELQGWRDAIKKLRSLSKVSVPIHFNVYKTEEITKQIFDKLLHLKLVAQNKYLLEMPLRL 179
           +++  WR A+ +  +LS   +   +   + +EIT  IF +L   +L A    L+ +   +
Sbjct: 150 DKIPRWRTALTEAANLSGWPLQDGYESNQIKEITDSIFRRLKCKRLDA-GANLVGIDSHV 209

Query: 180 NRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNSCFV-----RIAGHNL 239
             M     +  S +R +G+ G+GGIGKTTIA+ +Y++ + +F    F+     +     +
Sbjct: 210 KEMIWRLHMESSDVRMVGMYGVGGIGKTTIAKVIYNELSREFEYMSFLENIREKFNTQGV 269

Query: 240 VSLQQQLLSQLLTKD--IKISDENHGLRMIIDYLTSHKKVFIVFDGIIENRQLEMLVGNP 299
             LQ QLL  +L  +    I+   HG  MI D L+S K VFIV D + +  QLE L+ + 
Sbjct: 270 SPLQNQLLDDILKGEGSQNINSVAHGASMIKDILSS-KIVFIVLDDVDDQSQLEYLLRHR 329

Query: 300 NWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGDDPPNENLK 359
            W    SR+I+TTRNK +L      D    Y V+ L+   A  LF  +AF  + P  + +
Sbjct: 330 EWLGEGSRVIITTRNKHVLDVQKVDDL---YEVKGLNFEEACELFSLYAFEQNLPKSDYR 389

Query: 360 DLCNEII-----------------------------EKIGRHPLA--------------- 419
           +L + ++                              K+ R P A               
Sbjct: 390 NLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWESELRKLDREPEAEILSVLKRSYDGLGR 449

Query: 420 -----LVKIASSLYGQD--------------------------------EQIQMHILNIY 479
                 + +A    G+D                                 +I+MH L   
Sbjct: 450 TEKSIFLDVACFFKGEDRDFVSKILDACDFHAEIGIKNLNDKCLITLQYNRIRMHDLIQQ 509

Query: 480 MGQEIVRREM--GTHRQSRIWLREDVRRLFDENYELKYIQGIVMDLEEEEELVLEAKSLT 539
           MG EIVR +     ++ SR+W   D  R       +K ++ I +DL + + +   + +  
Sbjct: 510 MGWEIVREKFPDEPNKWSRLWDTCDFERALTAYKGIKRVETISLDLSKLKRVCSNSNAFA 569

Query: 540 DMSELKILQI-----------------------------NNVRLNEDIECLSNKLTLLNW 599
            M+ L++L++                             + +RL    +  S +L  L W
Sbjct: 570 KMTRLRLLKVQSSLDIDFEPEYIDADDKVELYDVVMKNASKMRLGRGFKFPSYELRYLRW 629

Query: 600 PGYPSKNLPSTFQPPPLLQLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEV 659
            GYP   LPS F    L++LHL  SN+++L  G K  + LK ID+S S  L +  +FS +
Sbjct: 630 DGYPLDFLPSNFDGGKLVELHLKCSNIKQLRLGNKDLEMLKVIDLSYSRKLSQMSEFSSM 689

Query: 660 RNLRRLILRNCGRLHKVHSSINRLERLVLLDMEGCVSFTSLPVEMSSLSCLKTLILNGCK 719
            NL RL LR C  L  +H S+  +++L  L ++ C    +LP  +  L  L+ L L  C 
Sbjct: 690 PNLERLFLRGCVSLIDIHPSVGNMKKLTTLSLKSCKKLKNLPDSIGDLESLEILDLAYCS 749

Query: 720 DLDQIPPSLGNVEPLEELDIGGTSISVIPFLKNLRILNCERLESNIWHSLAGSTHCFRSL 728
             ++ P   GN++ L ELD+  T+I  +P                       S     SL
Sbjct: 750 KFEKFPEKGGNMKSLTELDLQNTAIKDLP----------------------DSIGDLESL 809

BLAST of HG10003521 vs. ExPASy Swiss-Prot
Match: F4J339 (Probable disease resistance protein RPP1 OS=Arabidopsis thaliana OX=3702 GN=RPP1 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 2.4e-52
Identity = 217/816 (26.59%), Postives = 340/816 (41.67%), Query Frame = 0

Query: 112  KKNRSSVVEELQGWRDAIKKLRSLSKVSVPIHFNVYKT-----EEITKQIFDKLLHLKLV 171
            K  R    E+++ WR A++ + +++      H + ++      E+I+  + + L      
Sbjct: 211  KTCRGKPKEQVERWRKALEDVATIA----GYHSHSWRNEADMIEKISTDVSNMLNSFTPS 270

Query: 172  AQNKYLLEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKF-TNSC 231
                 L+ M   ++ ++    L L  +R IGI G  GIGKTTIA  L+++ + +F  ++ 
Sbjct: 271  RDFDGLVGMRAHMDMLEQLLRLDLDEVRMIGIWGPPGIGKTTIARFLFNQVSDRFQLSAI 330

Query: 232  FVRIAG----------HNLVSLQQQLLSQLLT-KDIKISDENHGLRMIIDYLTSHKKVFI 291
             V I G             + LQ Q+LSQ++  KDI IS        + D     KKVF+
Sbjct: 331  MVNIKGCYPRPCFDEYSAQLQLQNQMLSQMINHKDIMISHLGVAQERLRD-----KKVFL 390

Query: 292  VFDGIIENRQLEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSAS 351
            V D + +  QL+ L     WF   SRII+TT +  +L+        H Y VE  S+  A 
Sbjct: 391  VLDEVDQLGQLDALAKETRWFGPGSRIIITTEDLGVLKAHGIN---HVYKVEYPSNDEAF 450

Query: 352  SLFCKHAFGDDPPNENLKDLCNEIIEKIGRHPLALVKIASSLYGQD-------------- 411
             +FC +AFG   P+E   ++  E+    G  PL L  + S+L G+               
Sbjct: 451  QIFCMNAFGQKQPHEGFDEIAWEVTCLAGELPLGLKVLGSALRGKSKREWERTLPRLKTS 510

Query: 412  ------------------------------------------------------------ 471
                                                                        
Sbjct: 511  LDGKIGSIIQFSYDVLCDEDKYLFLYIACLFNGESTTKVKELLGKFLDVKQGLHLLAQKS 570

Query: 472  ------EQIQMHILNIYMGQEIVRREMGTH----RQSRIWLREDVRRLFDENYELKYIQG 531
                  E+I MH L    G+E  R++   H    RQ  +  R     L D+  + +   G
Sbjct: 571  LISFDGERIHMHTLLEQFGRETSRKQFVHHGFTKRQLLVGARGICEVLDDDTTDSRRFIG 630

Query: 532  IVMDLEE-EEELVLEAKSLTDMSELKILQIN----NVRLN---EDIECLSNKLTLLNWPG 591
            I ++L   EEEL +  K L  + +   ++I+      RL    +D+   S K+  LNW G
Sbjct: 631  IHLELSNTEEELNISEKVLERVHDFHFVRIDASFQPERLQLALQDLIYHSPKIRSLNWYG 690

Query: 592  YPSKNLPSTFQPPPLLQLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEVRN 651
            Y S  LPSTF P  L++L +  SN+ +LW G K+ +NLK +D+S S YL E P+ S   N
Sbjct: 691  YESLCLPSTFNPEFLVELDMRSSNLRKLWEGTKQLRNLKWMDLSYSSYLKELPNLSTATN 750

Query: 652  LRRLILRNCGRLHKVHSSINRLERLVLLDMEGCVSFTSLPV--------EMSSLSC---- 711
            L  L LRNC  L ++ SSI +L  L +LD+E C S   LP         E+   +C    
Sbjct: 751  LEELKLRNCSSLVELPSSIEKLTSLQILDLENCSSLEKLPAIENATKLRELKLQNCSSLI 810

Query: 712  -----------LKTLILNGCKDLDQIPPSLGNVEPLEELDIGG--------TSISVIPFL 742
                       LK L ++GC  L ++P S+G++  LE  D+          +SI  +  L
Sbjct: 811  ELPLSIGTATNLKQLNISGCSSLVKLPSSIGDITDLEVFDLSNCSSLVTLPSSIGNLQNL 870

BLAST of HG10003521 vs. ExPASy TrEMBL
Match: A0A5A7TDI7 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001270 PE=4 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 3.5e-224
Identity = 502/1091 (46.01%), Postives = 573/1091 (52.52%), Query Frame = 0

Query: 120  EELQGWRDAIKKLRSLSKVSVPIHFNVYKTEEITKQIFDKLLHLKLVAQNKYLLEMPLRL 179
            EELQ WR+AI ++ SLS V++             K I+DKLLHLK VA+++YL EMPLRL
Sbjct: 160  EELQRWREAISQICSLSGVAL----RPTSLMRTIKLIYDKLLHLKSVAEDRYLFEMPLRL 219

Query: 180  NRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNS-CFVRIAGHNLVSLQ 239
              M+M  G     +RFIGIVGMGGIGKTTIA+ +Y KFA KF N+ CF+ IAG N+VSLQ
Sbjct: 220  RTMEMLLGSISDDVRFIGIVGMGGIGKTTIAQFVYQKFAPKFRNNCCFLHIAGSNIVSLQ 279

Query: 240  QQLLSQL-LTKDIKISDENHGLRMIIDYLTSHKKVFIVFDGIIENRQLEMLVGNPNWFSS 299
            QQLLSQ+   +DIKI DE  G+  I   L S +KV  +FDGI +  QLEML GNP+W  +
Sbjct: 280  QQLLSQVFFLEDIKIVDEILGVNRIKHNLKSCQKVLFIFDGISKKSQLEMLAGNPDWLPA 339

Query: 300  RSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGDDPPNENLKDLCNE 359
             SRII+TTRNKDILRQ+N++DKV EY+ ELLS TSA SLFCK+AFG+  P+EN K+L NE
Sbjct: 340  GSRIIITTRNKDILRQTNFKDKVQEYSAELLSHTSAVSLFCKYAFGECLPDENFKELSNE 399

Query: 360  IIEKIGRHPLALVKIASSLYGQ-------------------------------------- 419
            IIEK G  PLALV+IASSLYGQ                                      
Sbjct: 400  IIEKTGTLPLALVQIASSLYGQGIDVWEDTLKSFHKLVYDNIFSHVLKSSYEGLQAESQQ 459

Query: 420  ---------------------------------------------DEQIQMHILNIYMGQ 479
                                                         D+QIQMH+L + MGQ
Sbjct: 460  IFLDLACFLNGEKVDRVVEILEGFGYNSPRTKLQLLADRYLIDLSDDQIQMHVLILCMGQ 519

Query: 480  EIVRREMGTHRQSRIWLREDVRRLFDENYELKYIQGIVMDL-EEEEELVLEAKSLTDMSE 539
            EIV+RE+GTH+Q+RIW RED RRLF ENY LKYIQGI MDL EEEEELVLEAKSL DM E
Sbjct: 520  EIVQRELGTHQQTRIWQREDARRLFHENYGLKYIQGITMDLGEEEEELVLEAKSLADMIE 579

Query: 540  LKILQINNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQLHLPGSNVERLWNG 599
            LKILQINNV+++E+I+CLSNKLTLLNWPGYPSK LPSTFQPPPLL+L LPGSNV RLWNG
Sbjct: 580  LKILQINNVQISENIDCLSNKLTLLNWPGYPSKYLPSTFQPPPLLELRLPGSNVIRLWNG 639

Query: 600  RKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKVHSSINRLERLVLLDME 659
            RKKF NLKEID S S++L+ETPD SEV NL+RLIL+NC  L +VH+SIN LERLVLLDME
Sbjct: 640  RKKFGNLKEIDASDSKHLVETPDLSEVPNLQRLILQNCETLQRVHASINSLERLVLLDME 699

Query: 660  GCVS-------------------------------------------------------- 719
            GCVS                                                        
Sbjct: 700  GCVSLKRFSFPITCKRLKTLVLSYSGLEFFPEFGWWMEYLTELHIDGTSINQLSPSITYL 759

Query: 720  -------------FTSLPVEMSSLSCLKTLILNGCKDLDQIPPSLGNVEPLEELDIGGTS 779
                          +SLP E+  L  LKTL LNGC+ LD+IP SLG VEPLEELDIGGTS
Sbjct: 760  TSLVLLNLRNCIGLSSLPTEICCLCSLKTLTLNGCESLDKIPSSLGYVEPLEELDIGGTS 819

Query: 780  ISVIPFLKNLRILNCERLESNIWHSLAG-STHCFRSLKDLNLSDCNLANEDIPDDLDLFS 810
            IS IPFL+NLRILNCERL+SNIWHSLAG   H  RSLKDLNLSDCNL +EDIP+DL+LFS
Sbjct: 820  ISTIPFLENLRILNCERLKSNIWHSLAGLPAHYVRSLKDLNLSDCNLVDEDIPNDLELFS 879

BLAST of HG10003521 vs. ExPASy TrEMBL
Match: A0A7L9RV91 (Resistance gene-like protein OS=Cucumis melo OX=3656 GN=Prv PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 1.0e-215
Identity = 479/1050 (45.62%), Postives = 548/1050 (52.19%), Query Frame = 0

Query: 146  VYKTEEITKQIFDKLLHLKLVAQNKYLLEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIG 205
            +++ +   KQI D+LL LKL A+   L EMPLRL  M+M  GL  + IRFIGIVGM GIG
Sbjct: 209  MFQIKSTAKQIIDRLLSLKLEAKEGNLFEMPLRLRTMEMLLGLGSNDIRFIGIVGMSGIG 268

Query: 206  KTTIAEALYDKFAHKFTN-------SCFVRIAGHNLVSLQQQLLSQLLTK---DIKISDE 265
            KTT+AE +Y   AH F +        CF+   G ++VSLQQQLL QL +    DI+I DE
Sbjct: 269  KTTLAEVIY---AHSFKSLISDLGKRCFLHSRGRSIVSLQQQLLDQLASSKMIDIQILDE 328

Query: 266  NHGLRMIIDYLTSHKKVFIVFDGIIENRQLEMLVGNPNWFSSRSRIIVTTRNKDILRQSN 325
            NHG+R+I  +L   KKV IV DGI E  QLEML G+P+WF   SRII+TT NKDI R  N
Sbjct: 329  NHGVRLIKQHLRFLKKVLIVLDGISETSQLEMLAGSPDWFGKGSRIIITTTNKDIFRHPN 388

Query: 326  YQDKVHEYNVELLSDTSASSLFCKHAFGDDPPNENLKDLCNEIIEKIGRHPLALVKIASS 385
            ++DKV EYNVEL S  +A SLFCK AFGD PP+E++KDLCNEIIEK+GR PLAL KIASS
Sbjct: 389  FKDKVQEYNVELPSHEAAFSLFCKLAFGDYPPSEDMKDLCNEIIEKVGRLPLALEKIASS 448

Query: 386  LYGQ-------------------------------------------------------- 445
            LYG                                                         
Sbjct: 449  LYGHDMNIWEDTLKNFHKVVYDNIFSDILKSSYEGLEAESQQIFLDLACFLNGEKVDRVI 508

Query: 446  ---------------------------DEQIQMHILNIYMGQEIVRREMGTHRQSRIWLR 505
                                       D  IQMHIL + MG+EIVRR++GT +Q+RIWLR
Sbjct: 509  EILQGFGYSSPQTNLQLLVDRCLIDILDGHIQMHILILCMGKEIVRRKLGTCQQTRIWLR 568

Query: 506  EDVRRLFDENYELKYIQGIVMDLEEEEELVLEAKSLTDMSELKILQINNVRLNEDIECLS 565
            +D RRLF EN ELKYI GIVMDLEEEEELVL+AK+   MSELKIL+INNV+L+EDIE LS
Sbjct: 569  DDARRLFHENNELKYICGIVMDLEEEEELVLKAKAFAGMSELKILRINNVQLSEDIEFLS 628

Query: 566  NKLTLLNWPGYPSKNLPSTFQPPPLLQLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLI 625
            NKLTLLNWPGYPSK LPSTFQPP L++LHLPGSNVERLWNG +KFKNLKEID S S+YL+
Sbjct: 629  NKLTLLNWPGYPSKYLPSTFQPPSLIELHLPGSNVERLWNGTQKFKNLKEIDASDSKYLV 688

Query: 626  ETPDFSEVRNLRRLILRNCGRLHKVHSSINRLERLVLLDMEGCVSF-------------- 685
            ETP+FSE RNLRRLILRNCGRL +VHSSIN L RL+L DMEGCVSF              
Sbjct: 689  ETPNFSEARNLRRLILRNCGRLKEVHSSINSLHRLILFDMEGCVSFKSFSFVITCESLKT 748

Query: 686  -------------------------------------------------------TSLPV 745
                                                                   +SLP 
Sbjct: 749  LVLSNCGLEFFPEFGFPMGYLTELHIDGTSINELSPSIKNLLGLVLLNLGNCIRLSSLPT 808

Query: 746  EMSSLSCLKTLILNGCKDLDQIPPSLGNVEPLEELDIGGTSISVIPFLKNLRILNCERLE 805
            E+ SLS LKTLILNGCK+L ++PPSL  V+PLEELDIGGTSIS IPF++NLRILNCERL+
Sbjct: 809  EIGSLSSLKTLILNGCKNLHKLPPSLEYVKPLEELDIGGTSISTIPFVENLRILNCERLK 868

Query: 806  SNIWHSLAG-STHCFRSLKDLNLSDCNLANEDIPDDLDLFSSLEILDLSNNHFESLPESM 810
            S IWHSLA   T  F SLKDLNLSDCNL +EDIP DL+LFSSLEILDL +NHFE L ES+
Sbjct: 869  SIIWHSLASLPTEYFSSLKDLNLSDCNLVDEDIPSDLELFSSLEILDLGSNHFERLSESI 928

BLAST of HG10003521 vs. ExPASy TrEMBL
Match: A0A5A7T8X1 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001250 PE=4 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 4.4e-211
Identity = 467/1021 (45.74%), Postives = 532/1021 (52.11%), Query Frame = 0

Query: 175  MPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTN-------SCFV 234
            MPLRL  M+M  GL  + IRFIGIVGM GIGKTT+AE +Y   AH F +        CF+
Sbjct: 1    MPLRLRTMEMLLGLGSNDIRFIGIVGMSGIGKTTLAEVIY---AHSFKSLISDLGKRCFL 60

Query: 235  RIAGHNLVSLQQQLLSQLLTK---DIKISDENHGLRMIIDYLTSHKKVFIVFDGIIENRQ 294
               G ++VSLQQQLL QL +    DI+I DENHG+R+I  +L   KKV IV DGI E  Q
Sbjct: 61   HSRGRSIVSLQQQLLDQLASSKMIDIQILDENHGVRLIKQHLRFLKKVLIVLDGISETSQ 120

Query: 295  LEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGD 354
            LEML G+P+WF  RSRII+TT NKDI R  N++DKV EYNVELLS  +A SLFCK AFGD
Sbjct: 121  LEMLAGSPDWFGERSRIIITTTNKDIFRHPNFKDKVQEYNVELLSHEAAFSLFCKLAFGD 180

Query: 355  DPPNENLKDLCNEIIEKIGRHPLALVKIASSLYGQ------------------------- 414
             PP+E++KDLCNEIIEK+GR PLAL KIASSLYG                          
Sbjct: 181  YPPSEDMKDLCNEIIEKVGRLPLALEKIASSLYGHDMDIWEDTLKNFHKVVYDNIFSDIL 240

Query: 415  ----------------------------------------------------------DE 474
                                                                      D 
Sbjct: 241  KSSYEGLEAESQQIFLDLACFLNGEKVDRVIEILQGFGYSSPQTNLQLLVDRCLIDILDG 300

Query: 475  QIQMHILNIYMGQEIVRREMGTHRQSRIWLREDVRRLFDENYELKYIQGIVMDLEEEEEL 534
             IQMHIL + MG+EIVRR++GT +Q+RIWLR+D RRLF EN ELKYI GIVMDLEEEEEL
Sbjct: 301  HIQMHILILCMGKEIVRRKLGTRQQTRIWLRDDARRLFHENNELKYICGIVMDLEEEEEL 360

Query: 535  VLEAKSLTDMSELKILQINNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQLH 594
            VL+AK+   MSELKIL+INNV+L+EDIE LSNKLTLLNWPGYPSK LPSTFQPP L++LH
Sbjct: 361  VLKAKAFAGMSELKILRINNVQLSEDIEFLSNKLTLLNWPGYPSKYLPSTFQPPSLIELH 420

Query: 595  LPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKVHSSI 654
            LPGSNVERLWNG +KFKNLKEID S S+YL+ETP+FSE RNLRRLILRNCGRL +VHSSI
Sbjct: 421  LPGSNVERLWNGTQKFKNLKEIDASDSKYLVETPNFSEARNLRRLILRNCGRLKEVHSSI 480

Query: 655  NRLERLVLLDMEGCVSF------------------------------------------- 714
            N L RL+L DMEGCVSF                                           
Sbjct: 481  NSLHRLILFDMEGCVSFKSFSFVITCESLKTLVLSNCGLEFFPEFGFPMGYLTELHIDGT 540

Query: 715  --------------------------TSLPVEMSSLSCLKTLILNGCKDLDQIPPSLGNV 774
                                      +SLP E+ SLS LKTLILNGCK+L ++PPSL +V
Sbjct: 541  SINELSPSIKNLFGLVLLNLGNCIRLSSLPTEIGSLSSLKTLILNGCKNLHKLPPSLESV 600

Query: 775  EPLEELDIGGTSISVIPFLKNLRILNCERLESNIWHSLAG-STHCFRSLKDLNLSDCNLA 810
            +PLEELDIGGTSIS IP ++NLRILNCERL+S IWHSLA   T  F SLKDLNLSDCNL 
Sbjct: 601  KPLEELDIGGTSISTIPLVENLRILNCERLKSIIWHSLASLPTEYFSSLKDLNLSDCNLV 660

BLAST of HG10003521 vs. ExPASy TrEMBL
Match: A0A5D3BL21 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001210 PE=4 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 5.8e-211
Identity = 467/1021 (45.74%), Postives = 532/1021 (52.11%), Query Frame = 0

Query: 175  MPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTN-------SCFV 234
            MPLRL  M+M  GL  + IRFIGIVGM GIGKTT+AE +Y   AH F +        CF+
Sbjct: 1    MPLRLRTMEMLLGLGSNDIRFIGIVGMSGIGKTTLAEVIY---AHSFKSLISDLGKRCFL 60

Query: 235  RIAGHNLVSLQQQLLSQLLTK---DIKISDENHGLRMIIDYLTSHKKVFIVFDGIIENRQ 294
               G ++VSLQQQLL QL +    DI+I DENHG+R+I  +L   KKV IV DGI E  Q
Sbjct: 61   HSRGRSIVSLQQQLLDQLASSKMIDIQILDENHGVRLIKQHLRFLKKVLIVLDGISETSQ 120

Query: 295  LEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGD 354
            LEML G+P+WF  RSRII+TT NKDI R  N++DKV EYNVELLS  +A SLFCK AFGD
Sbjct: 121  LEMLAGSPDWFGERSRIIITTTNKDIFRHPNFKDKVQEYNVELLSHEAAFSLFCKLAFGD 180

Query: 355  DPPNENLKDLCNEIIEKIGRHPLALVKIASSLYGQ------------------------- 414
             PP+E++KDLCNEIIEK+GR PLAL KIASSLYG                          
Sbjct: 181  YPPSEDMKDLCNEIIEKVGRLPLALEKIASSLYGHDMDIWEDTLKNFHKVVYDNIFSDIL 240

Query: 415  ----------------------------------------------------------DE 474
                                                                      D 
Sbjct: 241  KSSYEGLEAESQQIFLDLACFLNGEKVDRVIEILQGFGYSSPQTNLQLLVDRCLIDILDG 300

Query: 475  QIQMHILNIYMGQEIVRREMGTHRQSRIWLREDVRRLFDENYELKYIQGIVMDLEEEEEL 534
             IQMHIL + MG+EIVRR++GT +Q+RIWLR+D RRLF EN ELKYI GIVMDLEEEEEL
Sbjct: 301  HIQMHILILCMGKEIVRRKLGTRQQTRIWLRDDARRLFHENNELKYICGIVMDLEEEEEL 360

Query: 535  VLEAKSLTDMSELKILQINNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQLH 594
            VL+AK+   MSELKIL+INNV+L+EDIE LSNKLTLLNWPGYPSK LPSTFQPP L++LH
Sbjct: 361  VLKAKAFAGMSELKILRINNVQLSEDIEFLSNKLTLLNWPGYPSKYLPSTFQPPSLIELH 420

Query: 595  LPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKVHSSI 654
            LPGSNVERLWNG +KFKNLKEID S S+YL+ETP+FSE RNLRRLILRNCGRL +VHSSI
Sbjct: 421  LPGSNVERLWNGTQKFKNLKEIDASDSKYLVETPNFSEARNLRRLILRNCGRLKEVHSSI 480

Query: 655  NRLERLVLLDMEGCVSF------------------------------------------- 714
            N L RL+L DMEGCVSF                                           
Sbjct: 481  NSLHRLILFDMEGCVSFKSFSFVITCESLKTLVLSNCGLEFFPEFGFPMGYLTELHIDGT 540

Query: 715  --------------------------TSLPVEMSSLSCLKTLILNGCKDLDQIPPSLGNV 774
                                      +SLP E+ SLS LKTLILNGCK+L ++PPSL +V
Sbjct: 541  SINELSPSIKNLFGLVLLNLGNCIRLSSLPTEIGSLSSLKTLILNGCKNLHKLPPSLESV 600

Query: 775  EPLEELDIGGTSISVIPFLKNLRILNCERLESNIWHSLAG-STHCFRSLKDLNLSDCNLA 810
            +PLEELDIGGTSIS IP ++NLRILNCERL+S IWHSLA   T  F SLKDLNLSDCNL 
Sbjct: 601  KPLEELDIGGTSISTIPLVENLRILNCERLKSIIWHSLASLPTEYFSSLKDLNLSDCNLV 660

BLAST of HG10003521 vs. ExPASy TrEMBL
Match: A0A1S4E2G9 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498647 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 9.3e-209
Identity = 467/1041 (44.86%), Postives = 541/1041 (51.97%), Query Frame = 0

Query: 150  EEITKQIFDKLLHLKLVAQNKYLLEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTI 209
            + I KQI D LL LKL A+   L EMP RL  M+M FGL  + IR IGIVGM GIGKTT+
Sbjct: 219  KSIAKQIIDHLLSLKLEAKEGTLFEMPPRLRTMEMLFGLGSNDIRVIGIVGMRGIGKTTL 278

Query: 210  AEALYDKFAHKFT--NSCFVRIAGHNLVSLQQQLLSQLLTKDI---KISDENHGLRMIID 269
            AE +++ +   F+    CF+ I G ++VSLQQQLL QL   +    ++ DE+  +  +++
Sbjct: 279  AEHIFNHYFKYFSVGKYCFLHIVGRSIVSLQQQLLDQLGCSNFFSYQLWDEDLLVIFMME 338

Query: 270  YLTSHKKVFIVFDGIIENRQLEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYN 329
             L+S K V IVFDGI E  QL+ML G+P+WF   SRII+TT NK+I R  N++DKV EYN
Sbjct: 339  CLSSLKNVLIVFDGISEISQLKMLAGSPDWFGEGSRIIITTTNKEIFRHPNFKDKVQEYN 398

Query: 330  VELLSDTSASSLFCKHAFGDDPPNENLKDLCNEIIEKIGRHPLALVKIASSLYGQ----- 389
            VELLS  +A SLFCK AFGD PP+E++KDLCNEIIEK+GR PLAL KIA SLYG      
Sbjct: 399  VELLSHEAAFSLFCKLAFGDHPPSEDMKDLCNEIIEKVGRLPLALEKIAFSLYGHDMDIW 458

Query: 390  ------------------------------------------------------------ 449
                                                                        
Sbjct: 459  EDTLKNFHKVVYDNIFSDILKSSYEGLEAESQQIFLDLACFLNGEKVDRVIEILQGFGYS 518

Query: 450  ------------------DEQIQMHILNIYMGQEIVRREMGTHRQSRIWLREDVRRLFDE 509
                              D  IQMHIL + MGQEIVRR+MG  +Q+RIWLR+D RR+F E
Sbjct: 519  SPQTNLQMLVDRCLIDILDGHIQMHILILCMGQEIVRRKMGNCQQTRIWLRDDARRIFHE 578

Query: 510  NYELKYIQGIVMDLEEEEELVLEAKSLTDMSELKILQINNVRLNEDIECLSNKLTLLNWP 569
            N ELKYI GIVMDLEEEEEL+L+AK   DMSELKIL+INNV+L+EDIE LSNKLTLLNWP
Sbjct: 579  NNELKYICGIVMDLEEEEELILKAKVFADMSELKILRINNVQLSEDIEFLSNKLTLLNWP 638

Query: 570  GYPSKNLPSTFQPPPLLQLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEVR 629
            GYPSK LPSTFQPP LL+LHLPGSNVERLWNG +KFKNLKEID S S+YL+ETP+FSE R
Sbjct: 639  GYPSKYLPSTFQPPSLLELHLPGSNVERLWNGTQKFKNLKEIDASDSKYLVETPNFSEAR 698

Query: 630  NLRRLILRNCGRLHKVHSSINRLERLVLLDMEGCVSF----------------------- 689
            NLRRLILRNCGRL +VHSSIN L RL+L D+EGCVSF                       
Sbjct: 699  NLRRLILRNCGRLKEVHSSINSLHRLILFDVEGCVSFKSFSFVITCESLKTLVLSNCGLE 758

Query: 690  ----------------------------------------------TSLPVEMSSLSCLK 749
                                                          +SLP E+ SLS LK
Sbjct: 759  FFPEFGFPMGYLTELHIDGTSINELSPSIKNLLGLVLLNLGNCIRLSSLPTEIGSLSSLK 818

Query: 750  TLILNGCKDLDQIPPSLGNVEPLEELDIGGTSISVIPFLKNLRILNCERLESNIWHSLAG 809
            TLILNGCK+L ++PPSL  V+PLEELDIGGTSIS IPF++NLRILNCERL+S IWHSLA 
Sbjct: 819  TLILNGCKNLHKLPPSLEYVKPLEELDIGGTSISTIPFVENLRILNCERLKSIIWHSLAS 878

BLAST of HG10003521 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 224.6 bits (571), Expect = 3.0e-58
Identity = 206/747 (27.58%), Postives = 327/747 (43.78%), Query Frame = 0

Query: 120 EELQGWRDAIKKLRSLSKVSVPIHFNVYKTEEITKQIFDKLLHLKLVAQNKYLLEMPLRL 179
           E++  W++A+KKL ++S        +    ++I K I DKL+       +K L+ M   +
Sbjct: 134 EKVGKWKEALKKLAAISGEDSRNWDDSKLIKKIVKDISDKLVSTSW-DDSKGLIGMSSHM 193

Query: 180 NRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNSCFVR-----IAGHNL 239
           + ++    +    +R +GI GMGG+GKTTIA+ LY++ + +F   CF+         + +
Sbjct: 194 DFLQSMISIVDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQFQVHCFMENVKEVCNRYGV 253

Query: 240 VSLQQQLLSQLLTKDIKISDENHGLRMIIDYLTSHKKVFIVFDGIIENRQLEMLVGNPNW 299
             LQ + L ++  +  K +  +     II     HK VFIV D +  + QL  LV    W
Sbjct: 254 RRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVKETGW 313

Query: 300 FSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGDD-PPNENLKD 359
           F   SRIIVTTR++ +L           Y V+ L    A  LFC +AF ++       ++
Sbjct: 314 FGPGSRIIVTTRDRHLLLSHGIN---LVYKVKCLPKKEALQLFCNYAFREEIILPHGFEE 373

Query: 360 LCNEIIEKIGRHPLALVKIASSLY-------------------------------GQDEQ 419
           L  + +      PLAL  + S LY                               G DEQ
Sbjct: 374 LSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARLKTYPHSDIMEVLRVSYDGLDEQ 433

Query: 420 --------------------------------------------------IQMHILNIYM 479
                                                             +++H L   M
Sbjct: 434 EKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEIGITILTEKSLIVESNGCVKIHDLLEQM 493

Query: 480 GQEIVRREMGTHRQSR--IWLREDVRRLFDENYELKYIQGIVMDLEEEEELVLEAKSLTD 539
           G+E+VR++   +   R  +W  ED+  L  EN   + ++GI ++L E  E+    ++   
Sbjct: 494 GRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGTQLVEGISLNLSEISEVFASDRAFEG 553

Query: 540 MSELKILQI--------NNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQLHL 599
           +S LK+L            V L   +  L  KL  L W GYP K +PS F P  L++L +
Sbjct: 554 LSNLKLLNFYDLSFDGETRVHLPNGLSYLPRKLRYLRWDGYPLKTMPSRFFPEFLVELCM 613

Query: 600 PGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKVHSSIN 659
             SN+E+LW+G +  +NLK++D+S  +YL+E PD S+  NL  L L  C  L +V  SI 
Sbjct: 614 SNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEVPDLSKATNLEELNLSYCQSLVEVTPSIK 673

Query: 660 RLERLVLLDMEGCVSFTSLPVEMSSLSCLKTLILNGCKDLDQIPPSLGNVEPL------- 719
            L+ L    +  C+    +P+ +  L  L+T+ ++GC  L   P    N   L       
Sbjct: 674 NLKGLSCFYLTNCIQLKDIPIGI-ILKSLETVGMSGCSSLKHFPEISWNTRRLYLSSTKI 733

Query: 720 EELDIGGTSISVIPFLKNLRILNCERLESNIWHSLAGSTHCFRSLKDLNLSDCNLANEDI 745
           EEL    +SIS +  L  L + +C+RL      +L        SLK LNL  C    E++
Sbjct: 734 EELP---SSISRLSCLVKLDMSDCQRL-----RTLPSYLGHLVSLKSLNLDGCRRL-ENL 793

BLAST of HG10003521 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 224.6 bits (571), Expect = 3.0e-58
Identity = 205/751 (27.30%), Postives = 321/751 (42.74%), Query Frame = 0

Query: 111 NKKNRSSVVEELQGWRDAIKKLRSLSKVSVPIHFNVYKTEEITKQIFDKLLHLKLVAQNK 170
           +K   S  + +L+ WR+A+ K+ ++S   +          +IT++I  K L  + +    
Sbjct: 128 SKHKNSHPLNKLKDWREALTKVANISGWDIKNRNEAECIADITREIL-KRLPCQYLHVPS 187

Query: 171 YLLEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNSCFVR-- 230
           Y + +  RL  +     +    +R I I GMGGIGKTT+A+  +++F+H F  S F+   
Sbjct: 188 YAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENF 247

Query: 231 ----IAGHNLVSLQQQLLSQLLTK-DIKISDENHGLRMIIDYLTSHKKVFIVFDGIIENR 290
                       LQ QLLS +L + DI+    +H ++         K+V +V D + +  
Sbjct: 248 REYSKKPEGRTHLQHQLLSDILRRNDIEFKGLDHAVKERF----RSKRVLLVVDDVDDVH 307

Query: 291 QLEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFG 350
           QL     + + F   SRII+TTRN  +L+Q   +     Y+ + L    +  LF  HAF 
Sbjct: 308 QLNSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEG---SYSPKELDGDESLELFSWHAFR 367

Query: 351 DDPPNENLKDLCNEIIEKIGRHPLAL---------------------------------- 410
              P +       E++      PLA+                                  
Sbjct: 368 TSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKL 427

Query: 411 ---------------VKIASSLYGQD--------------------------------EQ 470
                          + IA    G D                                  
Sbjct: 428 QISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNN 487

Query: 471 IQMHILNIYMGQEIVRR--EMGTHRQSRIWLREDVRRLFDENYELKYIQGIVMDLEEEEE 530
           I MH L   MG++IVR         +SR+W   DV  +  +      I+G+ +  +  + 
Sbjct: 488 IMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDF 547

Query: 531 LVLEAKSLTDMSELKILQINNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQL 590
              E ++   M EL++L++  V LN   E     L  L W G+  +  P       L  L
Sbjct: 548 QYFEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAAL 607

Query: 591 HLPGSNVERLWNGR---KKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKV 650
            L  SN++R W  +   +    +K +D+S S YL ETPDFS   N+ +LIL NC  L  V
Sbjct: 608 DLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLV 667

Query: 651 HSSINRLE-RLVLLDMEGCVSFTSLPVEMSSLSCLKTLILNGCKDLDQIPPSLGNVEPLE 710
           H SI  L+ +LVLL++  C+    LP E+  L  L++L L+ C  L+++  +LG +E L 
Sbjct: 668 HKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGELESLT 727

Query: 711 ELDIGGTSISVIPF-------LKNLRILNCERLES----NIWHSLAGSTHCFRS------ 749
            L    T++  IP        LK L +  C+ L S    N++   + S    R       
Sbjct: 728 TLLADFTALREIPSTINQLKKLKRLSLNGCKGLLSDDIDNLYSEKSHSVSLLRPVSLSGL 787

BLAST of HG10003521 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 224.6 bits (571), Expect = 3.0e-58
Identity = 205/751 (27.30%), Postives = 321/751 (42.74%), Query Frame = 0

Query: 111 NKKNRSSVVEELQGWRDAIKKLRSLSKVSVPIHFNVYKTEEITKQIFDKLLHLKLVAQNK 170
           +K   S  + +L+ WR+A+ K+ ++S   +          +IT++I  K L  + +    
Sbjct: 131 SKHKNSHPLNKLKDWREALTKVANISGWDIKNRNEAECIADITREIL-KRLPCQYLHVPS 190

Query: 171 YLLEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNSCFVR-- 230
           Y + +  RL  +     +    +R I I GMGGIGKTT+A+  +++F+H F  S F+   
Sbjct: 191 YAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENF 250

Query: 231 ----IAGHNLVSLQQQLLSQLLTK-DIKISDENHGLRMIIDYLTSHKKVFIVFDGIIENR 290
                       LQ QLLS +L + DI+    +H ++         K+V +V D + +  
Sbjct: 251 REYSKKPEGRTHLQHQLLSDILRRNDIEFKGLDHAVKERF----RSKRVLLVVDDVDDVH 310

Query: 291 QLEMLVGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFG 350
           QL     + + F   SRII+TTRN  +L+Q   +     Y+ + L    +  LF  HAF 
Sbjct: 311 QLNSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEG---SYSPKELDGDESLELFSWHAFR 370

Query: 351 DDPPNENLKDLCNEIIEKIGRHPLAL---------------------------------- 410
              P +       E++      PLA+                                  
Sbjct: 371 TSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKL 430

Query: 411 ---------------VKIASSLYGQD--------------------------------EQ 470
                          + IA    G D                                  
Sbjct: 431 QISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNN 490

Query: 471 IQMHILNIYMGQEIVRR--EMGTHRQSRIWLREDVRRLFDENYELKYIQGIVMDLEEEEE 530
           I MH L   MG++IVR         +SR+W   DV  +  +      I+G+ +  +  + 
Sbjct: 491 IMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDF 550

Query: 531 LVLEAKSLTDMSELKILQINNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQL 590
              E ++   M EL++L++  V LN   E     L  L W G+  +  P       L  L
Sbjct: 551 QYFEVEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAAL 610

Query: 591 HLPGSNVERLWNGR---KKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKV 650
            L  SN++R W  +   +    +K +D+S S YL ETPDFS   N+ +LIL NC  L  V
Sbjct: 611 DLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLV 670

Query: 651 HSSINRLE-RLVLLDMEGCVSFTSLPVEMSSLSCLKTLILNGCKDLDQIPPSLGNVEPLE 710
           H SI  L+ +LVLL++  C+    LP E+  L  L++L L+ C  L+++  +LG +E L 
Sbjct: 671 HKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGELESLT 730

Query: 711 ELDIGGTSISVIPF-------LKNLRILNCERLES----NIWHSLAGSTHCFRS------ 749
            L    T++  IP        LK L +  C+ L S    N++   + S    R       
Sbjct: 731 TLLADFTALREIPSTINQLKKLKRLSLNGCKGLLSDDIDNLYSEKSHSVSLLRPVSLSGL 790

BLAST of HG10003521 vs. TAIR 10
Match: AT1G63870.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 221.9 bits (564), Expect = 1.9e-57
Identity = 194/706 (27.48%), Postives = 323/706 (45.75%), Query Frame = 0

Query: 120 EELQGWRDAIKKLRSLSKVSVPIHFNVYK-TEEITKQIFDKLLHLKLVAQNKYLLEMPLR 179
           E+ Q W  A+K + +++        N  K  E+I + + DK L+         ++ +   
Sbjct: 138 EDKQNWSKALKDVGNIAGEDFLRWDNEAKMIEKIARDVSDK-LNATPSRDFNGMVGLEAH 197

Query: 180 LNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNSCFVRIAGHNLVS-- 239
           L  M+    L    ++ +GI G  GIGKTTIA AL  + ++KF  +CFV     + ++  
Sbjct: 198 LTEMESLLDLDYDGVKMVGISGPAGIGKTTIARALQSRLSNKFQLTCFVDNLKESFLNSL 257

Query: 240 ----LQQQLLSQLLTKDIKISDENHGLRM----IIDYLTSHKKVFIVFDGIIENRQLEML 299
               LQ+Q L+++L  D        G+R+    +I+     ++V I+ D +    QLE L
Sbjct: 258 DELRLQEQFLAKVLNHD--------GIRICHSGVIEERLCKQRVLIILDDVNHIMQLEAL 317

Query: 300 VGNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGDDPPN 359
                WF S SRI+VTT NK+IL+Q    D    Y+V   SD  A  + C++AF     +
Sbjct: 318 ANETTWFGSGSRIVVTTENKEILQQHGINDL---YHVGFPSDEQAFEILCRYAFRKTTLS 377

Query: 360 ENLKDLCNEIIEKIGRHPLALVKIASSLYGQDEQ-------------------------- 419
              + L   + +  G  PL L  + SSL G++E+                          
Sbjct: 378 HGFEKLARRVTKLCGNLPLGLRVLGSSLRGKNEEEWEEVIRRLETILDHQDIEEVLRVGY 437

Query: 420 ---------IQMHI--------------------LNIYMGQEIV----------RREMGT 479
                    + +HI                    L+I  G +I+           RE+  
Sbjct: 438 GSLHENEQSLFLHIAVFFNYTDGDLVKAMFTDNNLDIKHGLKILADKSLINISNNREIVI 497

Query: 480 HRQSRIWLREDVRRLFDENYEL-----------------KYIQGIVMDLEEEEELVLEAK 539
           H+  + + R+ V +     +++                 K + GI  D+   +E+V+  K
Sbjct: 498 HKLLQQFGRQAVHKEEPWKHKILIHAPEICDVLEYATGTKAMSGISFDISGVDEVVISGK 557

Query: 540 SLTDMSELKILQI--------NNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLL 599
           S   +  L+ L++        + V + E+ E    +L LL+W  YP K+LP TFQP  L+
Sbjct: 558 SFKRIPNLRFLKVFKSRDDGNDRVHIPEETE-FPRRLRLLHWEAYPCKSLPPTFQPQYLV 617

Query: 600 QLHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKVH 659
           +L++P S +E+LW G ++  +LK++++  S +L E PD S   NL R+ L  C  L ++ 
Sbjct: 618 ELYMPSSQLEKLWEGTQRLTHLKKMNLFASRHLKELPDLSNATNLERMDLSYCESLVEIP 677

Query: 660 SSINRLERLVLLDMEGCVSFTSLPVEMSSLSCLKTLILNGCKDLDQIPPSLGNVEPLEEL 719
           SS + L +L  L+M  C++   +P  M +L+ L+T+ + GC  L  IP    N+    +L
Sbjct: 678 SSFSHLHKLEWLEMNNCINLQVIPAHM-NLASLETVNMRGCSRLRNIPVMSTNI---TQL 737

Query: 720 DIGGTSISVIPFLKNLRILN-CERLESNIWHSLAGSTHCFRSLKDLNLSDCNLANEDIPD 723
            +  T++  +P   ++R  +  ERL  +    L G TH   SLK L+L D ++  E IP+
Sbjct: 738 YVSRTAVEGMP--PSIRFCSRLERLSISSSGKLKGITHLPISLKQLDLIDSDI--ETIPE 797

BLAST of HG10003521 vs. TAIR 10
Match: AT1G72840.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 218.8 bits (556), Expect = 1.6e-56
Identity = 214/742 (28.84%), Postives = 331/742 (44.61%), Query Frame = 0

Query: 120 EELQGWRDAIKKLRSLS-KVSVPIHFNVYKTEEITKQIFDKLLHLK------LVAQNKYL 179
           E++  WR A+ ++ +LS K S           E+   I  +L  +K      LV    ++
Sbjct: 138 EKVSKWRRALTQVANLSGKHSRNCVDEADMIAEVVGGISSRLPRMKSTDLINLVGMEAHM 197

Query: 180 LEMPLRLNRMKMFFGLSLSSIRFIGIVGMGGIGKTTIAEALYDKFAHKFTNSCFVR--IA 239
           ++M L LN      G     +  IGI GMGGIGK+TIA+ LYD+F+ +F   CF+     
Sbjct: 198 MKMTLLLN-----IGCE-DEVHMIGIWGMGGIGKSTIAKCLYDRFSRQFPAHCFLENVSK 257

Query: 240 GHNLVSLQQQLLSQLL-TKDIKISDENHGLRMIIDYLTSHKKVFIVFDGIIENRQLEMLV 299
           G+++  LQ++LLS +L  +D+++     G + I + L  H+KVF+V D + +  QL  L 
Sbjct: 258 GYDIKHLQKELLSHILYDEDVELWSMEAGSQEIKERL-GHQKVFVVLDNVDKVEQLHGLA 317

Query: 300 GNPNWFSSRSRIIVTTRNKDILRQSNYQDKVHEYNVELLSDTSASSLFCKHAFGDDPPNE 359
            +P+WF   SRII+TTR+K +L         + Y V+ L D  A  +F K AFG  PP++
Sbjct: 318 KDPSWFGPGSRIIITTRDKGLLNSCGVN---NIYEVKCLDDKDALQVFKKLAFGGRPPSD 377

Query: 360 NLKDLCNEIIEKIGRHPLALVKIASSLY-------------------------------- 419
             + L           P ALV  AS L                                 
Sbjct: 378 GFEQLFIRASRLAHGLPSALVAFASHLSAIVAIDEWEDELALLETFPQKNVQEILRASYD 437

Query: 420 GQDEQ-----------------------------------------------IQMHILNI 479
           G D+                                                I MHIL +
Sbjct: 438 GLDQYDKTVFLHVACFFNGGHLRYIRAFLKNCDARINHLAAKCLVNISIDGCISMHILLV 497

Query: 480 YMGQEIVRREMG--THRQSRIWLREDVRRLFDENYELKYIQGIVMDLEEEEELVLEAKS- 539
             G+EIVR+E      +Q  +W   ++  + D N   + ++G+ + L E  + +L   S 
Sbjct: 498 QTGREIVRQESDWRPSKQRFLWDPTEIHYVLDSNTGTRRVEGLSLHLCEMADTLLLRNSV 557

Query: 540 ---LTDMSELKILQ-----INNVRLNEDIECLSNKLTLLNWPGYPSKNLPSTFQPPPLLQ 599
              + +++ LK  Q     ++N++L  D   LS  L LL+W  YP   LP  F+P  +++
Sbjct: 558 FGPMHNLTFLKFFQHLGGNVSNLQLISDDYVLSRNLKLLHWDAYPLTILPPIFRPHTIIE 617

Query: 600 LHLPGSNVERLWNGRKKFKNLKEIDVSGSEYLIETPDFSEVRNLRRLILRNCGRLHKVHS 659
           L L  S +  LW+G K   NL+ +DV+GS  L E P+ S   NL  LIL +C  L ++  
Sbjct: 618 LSLRYSKLNSLWDGTKLLPNLRILDVTGSRNLRELPELSTAVNLEELILESCTSLVQIPE 677

Query: 660 SINR-------------LERLVLLD--MEGCVS-------FTSLPVEMSSLSCLKTLILN 719
           SINR             LE ++L++   E  +S         +LP   ++LS L  L + 
Sbjct: 678 SINRLYLRKLNMMYCDGLEGVILVNDLQEASLSRWGLKRIILNLPHSGATLSSLTDLAIQ 737

Query: 720 GCKDLDQIPPSLGNVEPLEELDIGGTSISVIPFLKN-----LRILNCERLESNI--WHSL 733
           G K   ++    G  + L    +  T+   +  L N     L+ L+ +R    +   +  
Sbjct: 738 G-KIFIKLSGLSGTGDHLSFSSVQKTAHQSVTHLLNSGFFGLKSLDIKRFSYRLDPVNFS 797