HG10003474 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003474
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Ribonuclease H-like superfamily protein
Location	Chr08: 1895060 .. 1895347 (+)
RNA-Seq Expression	HG10003474
Synteny	HG10003474

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCCAGAGTCTTGGGTTGCTTTGATTATGAAAAGTGTCTATTATGAAAATGGTGATATCTTCCCAGCTGAGTTAAGCTCTTCTTCATCGTATGCCTGGAAAAGTTTGATTTGGGGCAAAGATCTTCTTATTAAAGGACTTAGATACAGAGTGGGGAGTGGATCCTCGATTAAAGTGGGAGAAGACCCTTGGCTCCCCCGGCCGAGTACCTTTAAGCCAATTTTGATTAAGCCACAGTTTATGAATGCTTCAATGGCTGATTTTATAAGGGAGGATGACTTTTGA

mRNA sequence

ATGAAGCCAGAGTCTTGGGTTGCTTTGATTATGAAAAGTGTCTATTATGAAAATGGTGATATCTTCCCAGCTGAGTTAAGCTCTTCTTCATCGTATGCCTGGAAAAGTTTGATTTGGGGCAAAGATCTTCTTATTAAAGGACTTAGATACAGAGTGGGGAGTGGATCCTCGATTAAAGTGGGAGAAGACCCTTGGCTCCCCCGGCCGAGTACCTTTAAGCCAATTTTGATTAAGCCACAGTTTATGAATGCTTCAATGGCTGATTTTATAAGGGAGGATGACTTTTGA

Coding sequence (CDS)

ATGAAGCCAGAGTCTTGGGTTGCTTTGATTATGAAAAGTGTCTATTATGAAAATGGTGATATCTTCCCAGCTGAGTTAAGCTCTTCTTCATCGTATGCCTGGAAAAGTTTGATTTGGGGCAAAGATCTTCTTATTAAAGGACTTAGATACAGAGTGGGGAGTGGATCCTCGATTAAAGTGGGAGAAGACCCTTGGCTCCCCCGGCCGAGTACCTTTAAGCCAATTTTGATTAAGCCACAGTTTATGAATGCTTCAATGGCTGATTTTATAAGGGAGGATGACTTTTGA

Protein sequence

MKPESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGEDPWLPRPSTFKPILIKPQFMNASMADFIREDDF

Homology

[Close]

BLAST of HG10003474 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 99.8 bits (247), Expect = 1.4e-17
Identity = 42/91 (46.15%), Postives = 61/91 (67.03%), Query Frame = 0


Query: 3    PESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGE 62
            P   V+ ++K  Y+++  +  A  +S SSY WK  +WG+DLL+KGLR RVG+GS+IK   
Sbjct: 970  PNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVKGLRLRVGNGSTIKAFS 1029

Query: 63   DPWLPRPSTFKPILIKPQFMNASMADFIRED 94
            DPWLPRP+TFKP+      ++ ++A FI  D
Sbjct: 1030 DPWLPRPTTFKPLRFNNGALDTTVASFITAD 1060

[Close]

BLAST of HG10003474 vs. NCBI nr
Match: XP_030509050.1 (uncharacterized protein LOC115723712 [Cannabis sativa])

HSP 1 Score: 92.8 bits (229), Expect = 1.7e-15
Identity = 38/88 (43.18%), Postives = 55/88 (62.50%), Query Frame = 0


Query: 3   PESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGE 62
           P S +A ++KS Y+  G    A   +  S  WKS++WGK+LL+KGLR+R+GSG+ +    
Sbjct: 890 PSSLIARVLKSRYFATGVFLEATAGAQPSLTWKSIVWGKELLLKGLRWRIGSGADVNCVS 949

Query: 63  DPWLPRPSTFKPILIKPQFMNASMADFI 91
            PW+P PS FKP+L      +  +ADFI
Sbjct: 950 QPWIPGPSNFKPLLFLSPDRDVKVADFI 977

[Close]

BLAST of HG10003474 vs. NCBI nr
Match: XP_030508852.1 (uncharacterized protein LOC115723496 [Cannabis sativa])

HSP 1 Score: 89.7 bits (221), Expect = 1.4e-14
Identity = 41/90 (45.56%), Postives = 56/90 (62.22%), Query Frame = 0


Query: 3   PESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGE 62
           P S ++ +++  Y+ NG+   A L S+ S  W+SL+WGK+LL+KGLR+RVGSG  I    
Sbjct: 890 PNSLLSNLLRHRYFSNGNYLIAGLGSNPSLTWRSLVWGKELLLKGLRWRVGSGERINCKT 949

Query: 63  DPWLPRPSTFKPILIKPQFMNASMADFIRE 93
           D WLP  +TFKP   K    N  +AD I E
Sbjct: 950 DSWLPGHTTFKPYFFKGPDPNLLVADLITE 979

[Close]

BLAST of HG10003474 vs. NCBI nr
Match: XP_030502765.1 (uncharacterized protein LOC115717936 [Cannabis sativa])

HSP 1 Score: 89.4 bits (220), Expect = 1.9e-14
Identity = 39/90 (43.33%), Postives = 56/90 (62.22%), Query Frame = 0


Query: 3   PESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGE 62
           P S ++ +++  Y+ NG+   + L S+ S  W+SL WGK+LL+KGLR+RVGSG +I    
Sbjct: 779 PNSLLSKLLRHRYFSNGNFISSGLGSNPSLTWRSLFWGKELLLKGLRWRVGSGLTINCAT 838

Query: 63  DPWLPRPSTFKPILIKPQFMNASMADFIRE 93
           D W+P  +TFKP   K    N  +AD I E
Sbjct: 839 DAWMPGNTTFKPYFFKGADPNLMVADLISE 868

[Close]

BLAST of HG10003474 vs. NCBI nr
Match: PKA49974.1 (Putative ribonuclease H protein [Apostasia shenzhenica])

HSP 1 Score: 88.6 bits (218), Expect = 3.2e-14
Identity = 38/90 (42.22%), Postives = 60/90 (66.67%), Query Frame = 0


Query: 5   SWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGEDP 64
           S +A ++K+ Y+ N D   A+ S +SSY W+S++WG+DLL  GLR+R+G+G+S++V  DP
Sbjct: 303 SLMARVLKAKYFHNCDFLMAQASPNSSYIWRSILWGRDLLKLGLRWRIGNGNSVRVFGDP 362

Query: 65  WLPRPSTFKPILI-KPQFMNASMADFIRED 94
           WLPRP +F+ I    P      + + + ED
Sbjct: 363 WLPRPISFQAITSPNPNLAATRIVELLTED 392

[Close]

BLAST of HG10003474 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.5e-06
Identity = 21/74 (28.38%), Postives = 42/74 (56.76%), Query Frame = 0


Query: 2   KPESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVG 61
           +P + ++ +++S Y+ +  +    + +  SYAW+S+I G++LL +GL   +G G   KV 
Sbjct: 80  QPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGIHTKVW 139

Query: 62  EDPWLPRPSTFKPI 76
            D W+   +   P+
Sbjct: 140 LDRWIMDETPLPPL 153

[Close]

BLAST of HG10003474 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 6.7e-18
Identity = 42/91 (46.15%), Postives = 61/91 (67.03%), Query Frame = 0


Query: 3    PESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGE 62
            P   V+ ++K  Y+++  +  A  +S SSY WK  +WG+DLL+KGLR RVG+GS+IK   
Sbjct: 970  PNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVKGLRLRVGNGSTIKAFS 1029

Query: 63   DPWLPRPSTFKPILIKPQFMNASMADFIRED 94
            DPWLPRP+TFKP+      ++ ++A FI  D
Sbjct: 1030 DPWLPRPTTFKPLRFNNGALDTTVASFITAD 1060

[Close]

BLAST of HG10003474 vs. ExPASy TrEMBL
Match: A0A803Q6Z2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 8.2e-16
Identity = 38/88 (43.18%), Postives = 55/88 (62.50%), Query Frame = 0


Query: 3    PESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGE 62
            P S +A ++KS Y+  G    A   +  S  WKS++WGK+LL+KGLR+R+GSG+ +    
Sbjct: 1614 PSSLIARVLKSRYFATGVFLEATAGAQPSLTWKSIVWGKELLLKGLRWRIGSGADVNCVS 1673

Query: 63   DPWLPRPSTFKPILIKPQFMNASMADFI 91
             PW+P PS FKP+L      +  +ADFI
Sbjct: 1674 QPWIPGPSNFKPLLFLSPDRDVKVADFI 1701

[Close]

BLAST of HG10003474 vs. ExPASy TrEMBL
Match: A0A1S8ACU2 (Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 3.1e-15
Identity = 34/91 (37.36%), Postives = 61/91 (67.03%), Query Frame = 0


Query: 3   PESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGE 62
           PES +A I+++ Y++  D   A+L S  S+ W+S+IWG+ +++ G+R+R+G+G  +K+ +
Sbjct: 122 PESLMAKILQAKYFKGADFLQAKLGSKPSFVWRSIIWGRQVIVNGMRWRIGTGDRVKIYK 181

Query: 63  DPWLPRPSTFKPILIKPQFMNASMADFIRED 94
             W+PRP  FKPI      M+ ++A+ I E+
Sbjct: 182 SHWIPRPQAFKPISAPTLGMDCTVAELIDEN 212

[Close]

BLAST of HG10003474 vs. ExPASy TrEMBL
Match: A0A2I0A354 (Ribonuclease H protein OS=Apostasia shenzhenica OX=1088818 GN=AXF42_Ash017513 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.5e-14
Identity = 38/90 (42.22%), Postives = 60/90 (66.67%), Query Frame = 0


Query: 5   SWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGEDP 64
           S +A ++K+ Y+ N D   A+ S +SSY W+S++WG+DLL  GLR+R+G+G+S++V  DP
Sbjct: 303 SLMARVLKAKYFHNCDFLMAQASPNSSYIWRSILWGRDLLKLGLRWRIGNGNSVRVFGDP 362

Query: 65  WLPRPSTFKPILI-KPQFMNASMADFIRED 94
           WLPRP +F+ I    P      + + + ED
Sbjct: 363 WLPRPISFQAITSPNPNLAATRIVELLTED 392

[Close]

BLAST of HG10003474 vs. ExPASy TrEMBL
Match: A0A803PWX1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.0e-14
Identity = 33/71 (46.48%), Postives = 53/71 (74.65%), Query Frame = 0


Query: 3    PESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVGE 62
            P S  + ++K+ YY NG +  A+  + +S+ W+SL+WGK ++ KG R+R+G+G+S++V +
Sbjct: 1425 PNSLCSRVLKASYYPNGGVIEAKSGNHASFVWRSLVWGKKIIQKGYRWRIGNGNSVRVID 1484

Query: 63   DPWLPRPSTFK 74
            DPWLPRP TFK
Sbjct: 1485 DPWLPRPVTFK 1495

[Close]

BLAST of HG10003474 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 8.1e-08
Identity = 30/96 (31.25%), Postives = 49/96 (51.04%), Query Frame = 0


Query: 2   KPESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVG 61
           +PES +A + KS Y+   D   A L S  S+ WKS+   +++L +G R  VG+G  I + 
Sbjct: 79  RPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEILRQGARAVVGNGEDIIIW 138

Query: 62  EDPWL---PRPSTFKPILIKPQFMNASMADFIREDD 95
              WL   P  +  +   + PQ   AS++  ++  D
Sbjct: 139 RHKWLDSKPASAALRMQRVPPQ-EYASVSSILKVSD 173

[Close]

BLAST of HG10003474 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 52.8 bits (125), Expect = 1.8e-07
Identity = 21/74 (28.38%), Postives = 42/74 (56.76%), Query Frame = 0


Query: 2   KPESWVALIMKSVYYENGDIFPAELSSSSSYAWKSLIWGKDLLIKGLRYRVGSGSSIKVG 61
           +P + ++ +++S Y+ +  +    + +  SYAW+S+I G++LL +GL   +G G   KV 
Sbjct: 80  QPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGIHTKVW 139

Query: 62  EDPWLPRPSTFKPI 76
            D W+   +   P+
Sbjct: 140 LDRWIMDETPLPPL 153

The following BLAST results are available for this feature:

BLAST of HG10003474 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158377.1	1.4e-17	46.15	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
XP_030509050.1	1.7e-15	43.18	uncharacterized protein LOC115723712 [Cannabis sativa]	[more]
XP_030508852.1	1.4e-14	45.56	uncharacterized protein LOC115723496 [Cannabis sativa]	[more]
XP_030502765.1	1.9e-14	43.33	uncharacterized protein LOC115717936 [Cannabis sativa]	[more]
PKA49974.1	3.2e-14	42.22	Putative ribonuclease H protein [Apostasia shenzhenica]	[more]

BLAST of HG10003474 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93295	2.5e-06	28.38	Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of HG10003474 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DX30	6.7e-18	46.15	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A803Q6Z2	8.2e-16	43.18	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A1S8ACU2	3.1e-15	37.36	Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1	[more]
A0A2I0A354	1.5e-14	42.22	Ribonuclease H protein OS=Apostasia shenzhenica OX=1088818 GN=AXF42_Ash017513 PE...	[more]
A0A803PWX1	2.0e-14	46.48	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of HG10003474 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	8.1e-08	31.25	Ribonuclease H-like superfamily protein	[more]
ATMG00310.1	1.8e-07	28.38	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10003474.1	HG10003474.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0016740	transferase activity