HG10003393 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003393
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionThreonylcarbamoyladenosine tRNA methylthiotransferase
LocationChr08: 581130 .. 595471 (-)
RNA-Seq ExpressionHG10003393
SyntenyHG10003393
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGACATAGAAGACTTGCTCATCGGCGGCGGCGGAGCTCCCCCTGGTTACCGCCTTCCGATAACCGCAGTGGGCGTTAAGCCGAAGAAAAAGAATAAGCTCAAATCTAATCCTAATGCTGATGATTCCAGCAACGAGCTCTCCCGGACCCAAAATCCACTTGTACCTAAGATTCCAGGCACACAGGTCTCTCTCTTCATGCCTCCTTGTATCGTGTTGAATGTTTCTTTTTTTGTTTGTTTGATTTTCACATTTCTTTTGCGGACACTGATAACTTCCTACTTCGATGTAATTTGGCAGACAATTTATGTTAAAACCTTTGGATGTTCTCACAACCAGGTCAGTCCTTCACCCCCACGTATTAAAACGATTTTTATTATTGCCTGGGCTTAAATGCAGGGCTAAGGCTTTAATTCCGTTAAGAGTTCATTTGTTGTCTGATAAACACTTAATCCAATTAGAGGTTGATTGGTTATCAGCTTTTATCCTTGCAAATTTGTTAGGTTCTGAAACTGGATTTTACTATTTTCATTCTGGATTCCATTAATGTTTCGGCTTTTATGGGTTTTACCTAAGGCTTTCAAATGATGTAAATTGATTAGAATTAAAGCTTTCAGTGCAATGTGATAAATATAAAACTAGAACTCTTTTTGAGCAGATAGAATGCGTGACGCAGACATAATATTAAATCTATTATTTTTTCTTTTCCTAATGAAGAACAAAAAAAATCTATCCTTAATCATGACGTAATCCAAATTTTCAAGAGGAAAAGTCCTACTTGGAAGACAAATATAACAAGGCGTGCTACTAGCTGAATGGAAGAAAGTTGGAAATCATCGAATTATATTATTATTATTAGTTTTTTGCTCAAGTAAGACACTTCACGCACACTGTCTAGATGTTCATATATATATATATATATATATAATTGCACTCTTCATGAATTTACTTCTCTTTTTTCTTCATGGTCTGCAAAAAAAAAAAAAAAATTTAATGCAAAATAAGTTTCACTTGTTTTGGACGAACCTAAGGAATTCTGTTCTTGATTCTGTGGAAGTCTACAATAATCTTATTTCCAAAAGCACAAATATTTAATAGATGATTCACGTTCATGTTATCCTTTTTACGTAGAATGCATCAGGCATCGTGGGGGATGATGGAATGATGGGGTCTTCTCTTGCATCATTGGTGAATTAACTTTAAATAGTTTAACGAAATACATGGATCTTAGGGTGAATCCTAAAACCAAGTCAATATATGGAATTTCCTTCCCAAAAGAAGGTCCTCATTATTTGGATGCCAAATCCCATTTAACAAGGTGACTAATGTTGCTTCCTTTATTTCCTTCCCAAAAGAAGTTCGTCATTATTCTCTCAATAGTTGAAATCACTTTGGATGGAATTAGGAAGAGTGACATATAGTAAATAGGAAGATTCTCTAATACGGCTTTACATCCAGTTAGTCTTCCTCCACAAGATAAGTTAAAGCGCTTCCAACGGTCTAACTTTTTCTGAATCTTATCAATAATAGGCTGCCAAAATGAAACTTTCTTGGGATAACAATCCAAAGGAAGACCAATATATAGAAAGGGTAGTGATTCAACCTTACAATGTTGGCGAGAGGAAGTGGACATCATCTTATCTTCATCTACATTTATGCCAAACAATTCTGATTTTTCCTAGTTAACCTTCTGATCTGAACACCACTGGTCCACTTAAAGAGCTCGATTATCTTGATAAGCGCATCCAACATACCATCGTCGTAAATACAAAATATAAGTATATCATCTGCAAACTGAAGAATATAAACATGTACTTTGTCTTTACCAACAATAAAGCCTTCAGAGACTCCTTTCTCATGTATAAGGTCAATAAGTGCATATAGAACTTCGCTAACTAATAGGAATAAAAAGGGGGAAATTGGATCCCCATGTCTTATACCTTTAGTAGCTTGAATGCTCCCTCTTGGTCTTCCATTAATAAATGCTGTGAATTTAGGATTACGGACACAACCAATAATCCATGAAATCCATTTAGAGTTGAATCCTTTAAGCATGAGAACTTCTTCAAGGAAATTCCAATACACTCTATCAAATGCATTTTCCAAATTGAGCTTGAGAATCCAACCCTTCTTCTTCTTGGAACGATACTCCTCTACAATTTCATTAGCCACAAGGTTGGGGCCACAATAATTCTTTTAGAGAATATTACATTATATGTGGATGGAAAGGATTTGAACCCGACCTTAAAATTATTTTCCTCTTATCAATTATTGTTTTATATTGAGAATATAATCAGTACTCTCGACCAAGATGTGAGAGGTTCAAATTTTCCACTTTGCATGTTGAATTAAAAAAGAAAGAAAAAGAACATAATCAGTCAAAAGATGATAACTTGATAAGCCAAAAAATGACTTTTTTTTGTTTGATTAAAGATATTGCTAAAGCTTAACTATTTAAGAAAACATAAAGGGTGGTGACTTAGTATAATCCAGAAGGCATCATTTAGGCCCCATTTGATAACCATTTGAGTTTTTGTTTCTAAAAATTGTGTTTGTTTTCTCACTATTTCTTCATCATGTTTTCATCTTTCCTTAGTAAGCATTTAAATTCTAAGCCGAATTTTAAAAACAAAAACTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATATATATATATAGTTTTCAAAACATGGCTTGTATTTAGTTGTCTTTATAATTTGGAGAATAAGGAACAAAAGGAGGCTAGTATGCTCTCTCCTTTCTCTTCTTTCTCCTTCGAAAATAACTTTGGTGGTTTTGTCTTCTTCTCTGATCAACTTTTCCCCTTGATTCTTTTGCACATCATGGAAGTTGGAAGCTGTAAGATCAATGATGCATTTTTTTTTTGTTTCTGGTTCGAGCATGGTAGCAATCATTTGGAAGCCATGACCCATAAGCAAGTGATTCCTCTTTCCAAGTCGCATCTTGTTTGGCTCGAGAATGCCATGGTTGAGCTACTCCATCAGCAACTTCATCAGTTTTATAAGAAACAACATCGATACTCCTCTGGTTCCATTCGTTTGACTAAAATGAAATCATCCTTTGGTTGGCAGTTTGGCCTTATTTTGGAGGGAGAAATTTCATTCATGTACCTCTGGGATTTTCTCAACATGTGGGGTGGTCACTAAAGGATATGGTTAGTAATTTTTTCAAATCAAACAAGAGACGCCAACCTAAAAGGAAAGTCCCCAATGTACTCTGCAACGCTAATGCCTTCCCCTATTGAAGTTTCTGTTGATCGAAGCTATGAGAGGTTGGATTCAACAAAGTTAACAAGGAAGATAGAAAGCAAAGTGTTCAATGATCAAGGAATTGTTAAGCAAACATCTTTTTGGGTTCAAAAAGAGAAAGAAGCCCTTTAGAAGAATTTTGACGAGTTATGAGTTGTATCACGTTTCTTTGTGCATAACCATTGGAAAGAGAGTGGAAGGGTGTTGGAAAGTGTTTTTCAATGTAATGTGATGCTTAAGCCTCTTGTTGCTGATAAGGCTTTAATCAACTATGATGGTAAGAGTTTTTCTAGCCTCATTGAAGCTCGTGGTTAGTGGAAGGCATCTAAGAAGTACATATACCGACACGCAACACGAATACGACACGACACACCAATTCCCAAAAATATTGGAGACGACACGTTGGGGACACGTTATTTTTTATTTTTTACAAAAACAAAAATATGTTGTGCATATAAACATATTGAAATATTACCTTTAAAGTTTAAACATTGAAATAATAACCATAGAATTCATGAAAAAGACTATAGATAAGATGACCAACGTCATAAGTTAGAACAATATTCAAGTGCCACTAAGTTAGTAATTAGTTTAACACAAAAAAACTACAAAGTAGAATCAAACAAGCCTTCTACATATTTTCCTCATTCTTGTCCTTTTCACAAATTTGAGTTTGATCCTCATCACCGTCACAATAAAAAGTATAGCTTCCAATTCTGGTTCGTTCAGGGATAAACTAGCTACTTCAAGTATTCTTGCATCTTCGAATGAATCAAAAGAATATCCAGCAGTGTCCCACATTTTAGTCTCTCATTTTGAATATTAAGGAGTTCTTGATAAAAGACGACGATTGCTATAGATATACACCAAGTCTTTTGCACGTTGTGGTGTCATCTTGTTCCTTCTCATGGAATTCACAAATGAATATGTACTCTAGTTTCTCTCACCACATGAGGAAGATGATGGTTGCACGAAGTAACTTCATAGTTAGTGGCTGTGGAGTTGGAGTAAGGCACCATGGATGGCCCACCAACTCTTAGGATCCATAAGATATCTATCACGTATAGAATCATGTTCAACAAACTCTGTAGACATTGTAGAGAAATTGGCAAATTGTATGCTCACTCTTGTGCGCTCATCCTTTGAGCTAAAACATCTCTTGATACTTCTAAATCTTTATGTGGTGGTTATTATTTGAAATGTCCTACCTCATTTATGTCCAAATTTAATTCAAGATAAATGTTAGATTCTATCCAAAATTTGATTTAGATTTGGAATAGAATTTTGAAATGGACTTTGTGGAATTTGATTTGGGATATAAATTTGATTATGTCCAAATTCAGTTTGATTCAAATTGAGTCAAATTCAGTATTAATATGACTTATCTTTTGACTTAAAATTGACAAGTTTTTAAGTTATAATTTGAATAAAAATTCCTAATTTGACAAGATTCCAAATTGGTACGTTTGTTTCTAATAATTTTCTTTATTAATTTATTTTCAATATTACTCTCTCACTCTCTCTCTTTTGGTGGAGTTGTTGGAAACATTAGAGGGAGGGAGAGAGAGAAAGAGAGGAAGATGAAGTTTTTCAATAAATATACAAACTTCAATAGTGAACACGGTTTAAATTGGTGGAATTGAGTTATTTAATGCTGAGTTGTAAAGTATCTGGATCAAACAATGAGTGAGTTGGTTAATTTATTTTTTAATGAACTCAATCCAGCTGAAATTGGTGGGCCAAACACCCCCTAAAACTGTAGTAATCTTAGGGTCATCACACACACTTTAGAGATTTGGCTTTGCTAGGGTGGTCTAGAGTTGCTTCTATTAAGGCTTTTAGCTTTAGCTTTGCCAGCTCGTAGAGTTGTAGGGAGACGATCATAAAGCTTCTTCTTTACTTGTCTTTCCATGAGAAAGAGTTGTTTTTGTAGTAGATTGGGGTTTGTTCCATTTTGTGAGGTCTTTAGTGTGAGAGAAATAATAGTATCTTCAAGAGGCGGGATGCTTGGTCCCTTATTAGATTCTACTTCTACATCTCTCTGTGGACATTGGTGGCTAAGTTTTTTCATAATTATTTGCTAGGTCTTATTTTACTGGATTAAAGCCTCTTTCTGTAGCTTGTTGCTTGGCTCCCCCTATTATTGTGGCTTTTTTCTTCCTTTTTGTATGCGCTGGTATTCTTTCACTTTTTCTCGATAAAAGTCTAGTTTATCATAAAAATAATGATATCCAAACCTGTCATGCATTATCTATTATTATATTTACATATATTTTGGAGAAAAATCTACCATCAACTTTCTTATTGGGTATTGCAACTTCCGCTGTTGTGAACAATGCAAAACACTGGACATAACTTTTTACAAAATATACTTAAGTAGCTATTTCTTTATTTAAATACCATTTCCTGTAAATGTTTGGTGTTAATACGCCAATTTGTTTTCTATTATTAATCTTAACAAAACTTTAATATGTGAGGTTGCTTATATTAACTGTATTAGTTGTGTCACCATTGCTAAAATAAAAATTGGGTACAATTTTCATCATTGTTGTCCTCATTGACAGAGTGACAGCGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGCGACAATCCTGAAGATGCAGACTTGTGGCTTATAAATACGTATGATCTGTTCCTTCAAGTTCTAAGTTTCGGATTTTGTCTTAATTTTACAGAAAATGTTCCATGAAAGTTTCTTTCTTAGTTGCAAAAAAGCATAAATTGCCTGTGAATGCTTTCAGGTGCTTTAATTGATTCAGTGATCAAAGAATTTTTGTGCCTCTAGCGTGCATTGTTACTTGGGTGTGGGGTGTGGGCCTTAGTATACTCTTGGCAGCATTTATTCAATAAATTTTTGTCTCTTTCCCATGTTGGTTATTACATCTTCTTGAGGACATTTACCCTCAACGTCCTCTTTATAGTGTCAGCTCTGCATTTATGTGCTTTGACATAGATAGCTGGCATATATGTGCTTTGGGACATACCTATAATTTTTCATATACTGGATGAAATATTTTTCTTCCTAAAGTATAAAAGGGTCAATATTTGGAATTTTGGACTAATAGCCTGATAAATATTTCTGCAGTTGCACAGTTAAATCCCCTAGTCAGTCTGCTATGGACACTCTAATAACAAAATGCAAAAATGCGAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTCGAGATTTAAAGGAGTTAGAAGGGGTCAGTATTGTAGGAGTTCAGCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAAGGTCATGAAGTAAGGCTGTTAAATCGCAAGACACTGCCAGCACTTGACCTCCCAAAAGTGTGAATTGTTTCCTTTCAAGCTTTTTGAGCCTTCTATTTGAGTTTTATTTAAGAAGCTTCCTTAGCTTATATTTTCTTTTTACCCTTTTGCTAACTATTAAAGAAGTTTATTTAATTTTTTGAACAGGTGAGAAAGAACAAGTTCATTGAAATACTTCCAATCAATGTTGGCTGTTTAGGTGCTTGCACTTATTGCAAAACAAAACATGCTCGTGGTCACTTGGGAAGTTACACTGTAGATAGCCTTGTAAGGACTTTAAACTAATGTATGGGTTTTGTTAAACGTATATCCTCCATTTGTTTTCATTCAGAAACTTTATGTTCTGTCTGAAAAAGGATCTGTGAAAACTGTATTTGCAGGTAAAGCGTGTAAAAACTGTCATCAATGAAGGAGTTAGGGAAATATGGTTGAGCAGTGAAGACACTGGGGCGTATGGTACTTTGCACTACTTCCATCTGAGATTGCTTTTTATTTTTATAGTATATGGCAAGCTGATACCTTCTTGCTTGTAAATTTATATTGTGTCGATTGAGTTATTTTGTCAGAAGATTGGAAGATTACATTGAATCCACCTAAATATACGGAGTTGGATTTTCAAAAAATGTTGATCTTGATGAAAATTTATTGAAATTTTGTATGAAGATTATATTCTTTCCTCACAGTGGACAAATATCAGTTCTTTCAATTATCAATTTTTAAAATGGATGTGCATTATAAGAATTTATAAAAAATAGTTAAATTGTCTATTCCAGGTTAAAGAAGAAATTAAGTGTAAAACTAGAGTCAAGATTGGAGAGCTCGAGAAATTTGATTCGTCCATCAATTACAATAGATTTTCTTCCAAAAGCATGATGAAAGTGTTTATTGTAGAGTTTTACATGTCTTGTAGAGGAGCGTTTTGAACTGTTGTTAATTATTTTGTTATTCTTCTTTCCTTATGCTTTCCTGTGCTTCTGTTCGTACTATCATATTTCCACAATAGGTTGGTCTAACGATCAAAATGAAAGCTACAAATTGAGACACAATCAAAGGATGACCTTAGGTCTAGATCTTTACATTGGTTTAGGTGGTCGTTCATAAGATTAACCTGGACAAGGTGGCCAACCAACCAATGACCGGTTTTGTATCATAAATTCTACGATAATTTTTAAATGTTGGATGTGATACCATCAAATTTGAACGTTGAAATAATGCATATTATCCAAGGTTGCTAAAAATTAAAATTGTAAAACATTTGTTCTTGCTAATGCCATATGATGAAATTTCAGGCCGTGACATTGGGGTCAATCTTCCAATTTTATTAAATGCAATTGTTGCTGAGCTTCCACCTGATGCAAGCACAATGCTTCGGATTGGGATGACAAATCCTCCTTTCATTCTTGAGCACTTGAAAGAGATAGCAAATGTCTTGTCTCATCCATGCGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGAAGTGATACAATCTTAAGTGTAAGTATTGTCTCCACCTAAATTTCCTTATATTTTTGGTGAATTCTTGATGCCAGTACAGTTTTGAGGAACAATGGTTATATTTTCTCTCAGGCAATGAATCGAGAATATACAGTCAGTGAGTTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGGTTGCAACGGATATAATATGTGGATTTCCCGGTTAGTAAGTGGGCTTAAGGCTATGTATTGATTCCTGTACTCGTATCTTTGTCTCCCTGTATGTGTGCATGGTTTTTTTGTTCCTTCTTTGCATACCGTTTTGGATGGGAAAAGATGCTTTCACATTTATTGATTTCTATGTTGCAAAACACCAATTACTGGAACAGTGTAATCTATAAATTATTTTCTTAAAAAGTGCAATGTATGAACAATTAACTTACAAACATTTTAAAATATAAATCTGTCCCAATGGTGAAACTATATTTGATTATTATGAAAATGTAACCCTTTGGCCTTGTGGAGATAACTCATCTCCCCTCTTTTTGTGATAAAACTCTGGTTAATCCTACTCTCTTTCCGCACACAAAAGAATTTTATTATTTTGAAAACACCTGAAAAATACGTTGGAGTCCCTGGTTGTGGAAAGTTCAAGCATGAATGGTTTGTTTTGGTCTCCATGCGGCAATGTGAAGATTTATTGTTTGTATGGCCATTTCCCCCCCTTTTTGCTTTGTATTGTCTTGCATGTTTGTTTCTCCATCAATGTTGTTTGTATACAGTATTGATTCACATTTTGGGTAGCATGTAATATACTAATATTTGAGGAGGAGTTGTTTTTGCTTTTGCTTTTGTGGTGGTTGTTTCCCCCCCTTTTTCATGGGGTATATGGATTGAGAGAAATAACATGATTATCAGAGAGAGATCGTGTGAGGAGGTATGGGAGGGGACTAGGCTAATGCCTTCTTGTAGGTGCCAGGTCATCGACATTTTTGTATATGATTTCTGTTTGATTTTATCATATTGAAGAACCTTTCTGTGGTAAGTTCTTAGGCTTCCTTTATTTTTTTAATTGTTATTGTTTTTTTATTTATTTTTTGGGTTGTTTTTTTGTATGCCCTTGTATTTCTTTTTGGTATCTCTACGAAAGTATTTTATTTTATTATTTTTTTAAAAAAATGATATTCCTAATCTTCATAATTAATTTTATTGTATGTGAATGATTGCCAAATTTATTGTCAATTCTGGTGCTTTTCAGGGGAAACAGATGAAGATTTTTGTGAAACTATCAACCTTATTAAAGAGTACAAATTACCTCAAGTTCACATCTCACAGTTCTATCCTCGACCAGGTATGCAAATGGCATCGTTGACACCTCTGTATTTCTTTGCATAGAATTTAAATACGGGATCAATTTGACTTGAATGCTAATGACTAGCTTAAGAATCCTCAATCTCAAAGGAGAACGAACCCTTGTGCTATTCCTTGTGTATTATTCAGGAGATGTCACAAGCTTAAGATTCTAAGGTTTATTTTCCAGGATGTGGTGATATGATTGCCTAGGTAGCATATATGCATTTTTTTTGTTAGTCCAAACACTTCTTGTATCCCATCCCATATAGTTTTCTTATTTTTGCTTGCAACTTATTTAATGAAGTGAGGAAAAAGGAGGTTTTCCTGTATTAATTTGTCTGATTCTTTTTCTTGTGGAAAAACAGAGCTTGAAAAAAAGGAAAAACGGTGTACAGAAACAACAACCTTGAGAAAGAGCTTGACCAAAAACAAGGTCTAACTAAACATAAAAGGGATTCAAACCAAAAGAATAAAACCGAAGGGATACATTCAACCAAAAATCCAAACAGGTTTAGTTTAAAAGGTTTTCCGTTAGGATGGCAGAACTTCCACATTTCTTTTTTCAATTTTAAAGAGGATCTGCTAATTATGGATCTCTGTGTTATTCAATCAACCTATTTTATACCTTTTCAGATGAAGCAGTGTGATAATAGCCCCAACAAATGAATGAATAACAGTGTGACAAGTTATTTATGCATGTGCAAACCAATTCTAGCATAGCTAGGTTGATAAGGCACCATTTACCATCTGAAGGTCAATAGTTTGATCTCCACCCGTGCAATTGTTGAACTAGAAAAGAAATAATAATAATAATATTCATGTGGATTAATTAAAGAGTTTGCATATGTTACCTTAGAAGAACCTTGGTTGTAGCAATACTTTCATTAAATTGATTGTCTTGAATATATCCGTCTAGACTATGCTTGATGTTTTTGGAGCTTCTCCTTTGTTTATAAATAAAGAGAATGTGATTGTATAAAACTAATATTTCCAGCGGCCAAACATTTAAAATGAAAAAAACACTATCTATTGTAATTCAGGGACACCTGCTGCTAGGATGAAGAAAGTACCAAGTGCTATTGTCAAGAAACGAAGCCGTGAGTTGACTTCTGTTTTTGAAGCTTTTACACCGTATAATGGAATGGAGGGCAGAGTGGAAAGAATTTGGATAACAGAAATTGCAGCAGATGGAGTTCACTTGGTAAGAAAGTGATGCTGCCTAATGTCTATCTTTTCTAATTCTACTAACTTGTAATTTGCCTACAGTTTAGTGGCTGGTTTCAGGTGACTACTACCAAAGAAAATAGTTAGGACAATGGTAAGAAATTTGTTTGGATCAGTTTTGGGCCGAATTTTGAGTCATCTAGGTTGTTTAATTGGACTTTTAGCTTAGCGGAAATGAGATTTGTTTACAGTGACAAACAAAATCAGGGACAAAATAGAATAGGGAACCAATAGAATCCACTGTTACAGTTTTAGAATCCATATAAAATTTTAGATTCAAGTCTGCGTGCCTTTACAAATTAATATTAGAAATTATCTACTGCAAGAGTAATTAAACATTTAAATTCACCGTGTTCTAAGTATGTTTTAGTAACTGCATTTGGACCGAGGAAAAGAACCCAGATGATTCGTTTTAGTAACTGCATTTGGGCGGAGGAAAAAAACGCAGCTGAACGATAATCATCGGTTGATCTTGGAGTGAATTAGAATTAGTTGACCTAACCAAAATTAGGATTTCAAGACGTTAAGGACTCTTGTATGTCTACATATCTAAAATATAATAACTACTAACTAGGCTCTAACTTTTTTCTTATAATTTATGATTATTTATTTTTTAACCAGAAAGGAAAATTTTCGTTGAATTTTGAAAAGAGACTATGCCTTAAAAGATATAGGGAAGGGAAAAGTAAAAAAGGAAAAAAGAATAATGAAGTATAAACATTCCTAGTTTAAACAACTACCTTGACCCTTTTTTTATATAAGCAACAACTTTCATTGAGATAAAAATAAAAGAACACATGGACATACAAAAAACTAGCCCACAAAAATAGGATCCCAACTACAAGAAGGGACTCTGTACAAAATAGATCCTATTGGATAGTTACAAATTTTGAAGACCAGAGGGAAACATGAAATCTAACAAGGGGTCACACCTAACTAGGATTCCTTTCCAATCCCTTAAACACACAATTATTCCGCTTTCCCAAAGATCCCACAAAATAGCACAAAACAATATATTTGTCAAGGTATTATATCAAATACCTTGACCATTAAGAAGCCTTCAATTTTGCCACCTCAAAGCAGTCAACCCAACCTTTAAATATAACTAGTTTTTGTTAGTCTTAATTAATTTTAAACGGGTTTTGTGTCCCCAACTCCCTTAATTAATTTTAAACAATCTTCAAAACAATGGATTTTCTTCAAACACGTTTTATAAGACATTGCTTCATTACAAAGTATGTAGCTGGCTTGTTCATGTGTGAGTTAAACGTAGTTATTCTTTCATATATGTATATGAAGTATACTAGTTATATTGCTTGGACCATTCAGGGTTGTGAAATGCCTGTTCTGAGTATGCTCTACACTATTGGACAACTTCACCGATTTGCTAAACAGCATGGCAGTGAACTATTTTCAGACTGTTGTAAGAGCTTTCCATCTATTCTTCCACGTTGGCTTAGGATTTGGTTGTTCTCCTTGGTCTTATAGGCTACGAATGCAGGTTCAGATGTTTGGCTTTTCCATTCGGGTGGAAGTTTTTGTTTAAATCATTATTGTGTAAGTTGGTTGCTATGAGGGAAGCTTTGAAACAAGATCTAGTTGAAGCATATTGAAAAAGAAAGGGACAGAAGGGCGGAGTTATTTATGTGTACATTAGCTTTTGAAGGACTGAATAGGGGTTGCAATTATAAAATAGGTGTCCTCGGCCTACCTTAAATCTAAGGGTGTGCATTTTGTTTTCTTTTTTTGGGGATAGAAACGGTTTTCATTGAAATAAAAATGAAGCTCCATCGTCCAATGCCTTTAGGCACAAAATTACTAGCTTGCTCTTTAGTTGTAGCTGGTGTTTTGATGGTAAAAGGTAAAAAATAGCAAGGAACAGTTGCGTGCAGACTAAGGATGAAAGATAAAGCTAAAACCCTGGTAAATGTAGTAGACACTATCTTGTGGTGAACTTGGATGGGAATAAATGGTAGAACCTTTAGAAGGTAAGCATGGATCGTGGGCTGACCTAGTGGTAAAAAAAGAGAGATAGTCTCAATAAATGGCTAAGAGGTCATGGGTTCACTCCATGATGGCCATTGACACTCAAATGTTGTAGGGTCATACAGGTTGTCCCGTGAAATTAGTCGAAGCGCGCGTAAGCTGGAGACTCACAGATATTAAAAAAAAACAAAAGAAAAAGATCGTGGGCTGAAATATTTGATTAAGCCAAGTTGTGATGCAGTAGGATTGGGGATAATCTTGTAATTTTTTGTTGAGTTTTTTAATTGTTAGTTCTAGTTGGGGCTAGTGGTTAATTGGTTGCTTTGTTTGTAATTGGGTTTTACTTAGCTCGTCAGTTGGCATCCAGAGGATACTCTTGTGTTTATCCTTGGGGTTCTATCTTTTGGATGCGAGGTTGCATCATTTTTTCTTTTTTTTTTGAATGGAGCACGGAAAGTAGTTGACCAAGAAAGATGACAAGCAAGAATCCAAAGTCCAAACGTGGTAGGAAATGAAAGTTCAGTGAGCTTTCAATAGTGCTATTGGAATAGCTTAGACCCATAAATCCTTGTCATAAAAATGGAACATAGACTTGAGCTTGAGAGTTTGCATGGGTTGCCAATTGAAAGAAATCAATGGCCAAGTAGGAAAAGGGAAGCAACTTGGATGGTAAGAGGTACAAAGAGTCAATCAAGACTCGAGCTATCCAGTCAGGAACGAAGGCCACCAAAAGCAAAGGAAATGTAATTTCTTCCCAACAACCACTTGACGCTGGAAGAAGGGTGGTCTGAAGCATTGACCTTTATTAAGCAAGGCTGGATATACTTGCATCAAGGATAGTACTTTGCAAACATGTGAAATGATTCTTGTGACTAACTCCTCTATCTCTTCTTTTCTCTGTTTCTTAATGGAAGCTGTATTTGTTCTTCCTCGTGTGAAAATGAACCATTGTCATTAAACCGCTTCTGAATCAATTTCAATTCCCTAGGGAACTACATTTCTCTGATCTGTACTCTTAAAATTGCTGTTTCTGTACGCTGTGATCTAATGTTTCAAAGAACTGCTGTTTCATTTTTTGATTAGCTCAATTTATTTTGCTTCCCTAGGTTGGCCATACCAAGGGATACATACAGGTGCTTGTAATTGCCCCGGAAACTATGCTGGGAACTTCTGCTATGGTGAAGATAACATCCATCGGAAGGTGGTCTGTTTTTGGGGAAGTGATCGAAACAATCAGTACCAAACATCATAAAACTACTACTCTGGAAGACACGCCGATTCAAGACAAAGTTCCTCCCTGCTCTAACGCTCACGAAACATGTGCATGTTCCATGGAACCCGAACCTTGTGGTTGTGGACCAGAAAGCTGCAAGGGAGCAGTGGCTGTGGATGATAAAGATGATTCCTCAAGGAATATCCCATTGGCTGAAGAGCCAAAACGGAAGAATCTGATTGAATGGCTATTGAGGAGACGGAAGAGCCATGTTCCCAAAAGTGAGGAGAGAGAAAGTACCATAGTAACTGAGAGAAAGCAGGGATCGGCTGGAGGAAGGCTGGATGAGTGGGGTGTTGTTGATAGGATTCTTGTTGGTGGAATACTAATCAGTACCTTAACAATTTTTGGTCTACTGTTTCATCTTGGATCCAAAACACTCTCCTCCAGTTGGTGA

mRNA sequence

ATGGAAGACATAGAAGACTTGCTCATCGGCGGCGGCGGAGCTCCCCCTGGTTACCGCCTTCCGATAACCGCAGTGGGCGTTAAGCCGAAGAAAAAGAATAAGCTCAAATCTAATCCTAATGCTGATGATTCCAGCAACGAGCTCTCCCGGACCCAAAATCCACTTGTACCTAAGATTCCAGGCACACAGACAATTTATGTTAAAACCTTTGGATGTTCTCACAACCAGAGTGACAGCGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGCGACAATCCTGAAGATGCAGACTTGTGGCTTATAAATACTTGCACAGTTAAATCCCCTAGTCAGTCTGCTATGGACACTCTAATAACAAAATGCAAAAATGCGAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTCGAGATTTAAAGGAGTTAGAAGGGGTCAGTATTGTAGGAGTTCAGCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAAGGTCATGAAGTAAGGCTGTTAAATCGCAAGACACTGCCAGCACTTGACCTCCCAAAAGTGAGAAAGAACAAGTTCATTGAAATACTTCCAATCAATGTTGGCTGTTTAGGTGCTTGCACTTATTGCAAAACAAAACATGCTCGTGGTCACTTGGGAAGTTACACTGTAGATAGCCTTGTAAAGCGTGTAAAAACTGTCATCAATGAAGGAGTTAGGGAAATATGGTTGAGCAGTGAAGACACTGGGGCGTATGGCCGTGACATTGGGGTCAATCTTCCAATTTTATTAAATGCAATTGTTGCTGAGCTTCCACCTGATGCAAGCACAATGCTTCGGATTGGGATGACAAATCCTCCTTTCATTCTTGAGCACTTGAAAGAGATAGCAAATGTCTTGTCTCATCCATGCGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGAAGTGATACAATCTTAAGTGCAATGAATCGAGAATATACAGTCAGTGAGTTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGGTTGCAACGGATATAATATGTGGATTTCCCGGGGAAACAGATGAAGATTTTTGTGAAACTATCAACCTTATTAAAGAGTACAAATTACCTCAAGTTCACATCTCACAGTTCTATCCTCGACCAGGGACACCTGCTGCTAGGATGAAGAAAGTACCAAGTGCTATTGTCAAGAAACGAAGCCGTGAGTTGACTTCTGTTTTTGAAGCTTTTACACCGTATAATGGAATGGAGGGCAGAGTGGAAAGAATTTGGATAACAGAAATTGCAGCAGATGGAGTTCACTTGGTTGGCCATACCAAGGGATACATACAGGTGCTTGTAATTGCCCCGGAAACTATGCTGGGAACTTCTGCTATGGTGAAGATAACATCCATCGGAAGGTGGTCTGTTTTTGGGGAAGTGATCGAAACAATCAGTACCAAACATCATAAAACTACTACTCTGGAAGACACGCCGATTCAAGACAAAGTTCCTCCCTGCTCTAACGCTCACGAAACATGTGCATGTTCCATGGAACCCGAACCTTGTGGTTGTGGACCAGAAAGCTGCAAGGGAGCAGTGGCTGTGGATGATAAAGATGATTCCTCAAGGAATATCCCATTGGCTGAAGAGCCAAAACGGAAGAATCTGATTGAATGGCTATTGAGGAGACGGAAGAGCCATGTTCCCAAAAGTGAGGAGAGAGAAAGTACCATAGTAACTGAGAGAAAGCAGGGATCGGCTGGAGGAAGGCTGGATGAGTGGGGTGTTGTTGATAGGATTCTTGTTGGTGGAATACTAATCAGTACCTTAACAATTTTTGGTCTACTGTTTCATCTTGGATCCAAAACACTCTCCTCCAGTTGGTGA

Coding sequence (CDS)

ATGGAAGACATAGAAGACTTGCTCATCGGCGGCGGCGGAGCTCCCCCTGGTTACCGCCTTCCGATAACCGCAGTGGGCGTTAAGCCGAAGAAAAAGAATAAGCTCAAATCTAATCCTAATGCTGATGATTCCAGCAACGAGCTCTCCCGGACCCAAAATCCACTTGTACCTAAGATTCCAGGCACACAGACAATTTATGTTAAAACCTTTGGATGTTCTCACAACCAGAGTGACAGCGAATACATGGCTGGTCAGCTTTCAGCATTTGGTTATTTGTTAAGCGACAATCCTGAAGATGCAGACTTGTGGCTTATAAATACTTGCACAGTTAAATCCCCTAGTCAGTCTGCTATGGACACTCTAATAACAAAATGCAAAAATGCGAAAAAGCCTCTTGTGGTAGCTGGGTGTGTACCACAAGGTAGTCGAGATTTAAAGGAGTTAGAAGGGGTCAGTATTGTAGGAGTTCAGCAGATTGACCGTGTGGTAGAAGTTGTGGAAGAGACACTGAAAGGTCATGAAGTAAGGCTGTTAAATCGCAAGACACTGCCAGCACTTGACCTCCCAAAAGTGAGAAAGAACAAGTTCATTGAAATACTTCCAATCAATGTTGGCTGTTTAGGTGCTTGCACTTATTGCAAAACAAAACATGCTCGTGGTCACTTGGGAAGTTACACTGTAGATAGCCTTGTAAAGCGTGTAAAAACTGTCATCAATGAAGGAGTTAGGGAAATATGGTTGAGCAGTGAAGACACTGGGGCGTATGGCCGTGACATTGGGGTCAATCTTCCAATTTTATTAAATGCAATTGTTGCTGAGCTTCCACCTGATGCAAGCACAATGCTTCGGATTGGGATGACAAATCCTCCTTTCATTCTTGAGCACTTGAAAGAGATAGCAAATGTCTTGTCTCATCCATGCGTATACTCCTTTTTGCATGTGCCAGTTCAATCTGGAAGTGATACAATCTTAAGTGCAATGAATCGAGAATATACAGTCAGTGAGTTCAGGACTGTAGTAGATACCTTAACTGAGCTTGTTCCTGGGATGCAGGTTGCAACGGATATAATATGTGGATTTCCCGGGGAAACAGATGAAGATTTTTGTGAAACTATCAACCTTATTAAAGAGTACAAATTACCTCAAGTTCACATCTCACAGTTCTATCCTCGACCAGGGACACCTGCTGCTAGGATGAAGAAAGTACCAAGTGCTATTGTCAAGAAACGAAGCCGTGAGTTGACTTCTGTTTTTGAAGCTTTTACACCGTATAATGGAATGGAGGGCAGAGTGGAAAGAATTTGGATAACAGAAATTGCAGCAGATGGAGTTCACTTGGTTGGCCATACCAAGGGATACATACAGGTGCTTGTAATTGCCCCGGAAACTATGCTGGGAACTTCTGCTATGGTGAAGATAACATCCATCGGAAGGTGGTCTGTTTTTGGGGAAGTGATCGAAACAATCAGTACCAAACATCATAAAACTACTACTCTGGAAGACACGCCGATTCAAGACAAAGTTCCTCCCTGCTCTAACGCTCACGAAACATGTGCATGTTCCATGGAACCCGAACCTTGTGGTTGTGGACCAGAAAGCTGCAAGGGAGCAGTGGCTGTGGATGATAAAGATGATTCCTCAAGGAATATCCCATTGGCTGAAGAGCCAAAACGGAAGAATCTGATTGAATGGCTATTGAGGAGACGGAAGAGCCATGTTCCCAAAAGTGAGGAGAGAGAAAGTACCATAGTAACTGAGAGAAAGCAGGGATCGGCTGGAGGAAGGCTGGATGAGTGGGGTGTTGTTGATAGGATTCTTGTTGGTGGAATACTAATCAGTACCTTAACAATTTTTGGTCTACTGTTTCATCTTGGATCCAAAACACTCTCCTCCAGTTGGTGA

Protein sequence

MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGRWSVFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPESCKGAVAVDDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHVPKSEERESTIVTERKQGSAGGRLDEWGVVDRILVGGILISTLTIFGLLFHLGSKTLSSSW
Homology
BLAST of HG10003393 vs. NCBI nr
Match: XP_038890156.1 (threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 1221.8 bits (3160), Expect = 0.0e+00
Identity = 608/633 (96.05%), Postives = 616/633 (97.31%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKIP 60
           MEDIEDLLIGGGGAPPGYRLPI AVGVKPKKKN LK NPNADDSSNELS TQNPLVPKIP
Sbjct: 1   MEDIEDLLIGGGGAPPGYRLPINAVGVKPKKKNMLKFNPNADDSSNELSPTQNPLVPKIP 60

Query: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120
           GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT
Sbjct: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120

Query: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180
           LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR
Sbjct: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180

Query: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVINE 240
           KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV++VINE
Sbjct: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSVINE 240

Query: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300
           GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA
Sbjct: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300

Query: 301 NVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF 360
           NVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF
Sbjct: 301 NVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF 360

Query: 361 PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420
           PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA
Sbjct: 361 PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420

Query: 421 FTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGRWS 480
           FTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVIAPETMLGTSAMVKITS GRWS
Sbjct: 421 FTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMVKITSFGRWS 480

Query: 481 VFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPESCKGAVAV 540
           VFGEVIETISTKHHKTTTLED PI+DKVPPCSNAHETCACS+EPE C CGPESCKGAVA 
Sbjct: 481 VFGEVIETISTKHHKTTTLEDAPIRDKVPPCSNAHETCACSIEPESCACGPESCKGAVAT 540

Query: 541 DDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHVPKSEERESTIVTERKQGSAGGRLDEWG 600
           DDKDDSSRNIPL EEPKRKNL+EW LRRRKSHVPK EERES IV+ERKQ  AGG LDEWG
Sbjct: 541 DDKDDSSRNIPLPEEPKRKNLVEWALRRRKSHVPKREERESPIVSERKQALAGGWLDEWG 600

Query: 601 VVDRILVGGILISTLTIFGLLFHLGSKTLSSSW 634
           V+DRILVGGILISTLTIFGLLFHLGS TLSSSW
Sbjct: 601 VIDRILVGGILISTLTIFGLLFHLGSTTLSSSW 633

BLAST of HG10003393 vs. NCBI nr
Match: XP_023524910.1 (threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 587/633 (92.73%), Postives = 604/633 (95.42%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKIP 60
           MEDIEDLLIGGGGAPPGYRLPI+AVGVKPKKKNKLK NP+ADDSS+ L  TQN L PKIP
Sbjct: 1   MEDIEDLLIGGGGAPPGYRLPISAVGVKPKKKNKLKPNPDADDSSSGLFPTQNLLAPKIP 60

Query: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120
           GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSAMDT
Sbjct: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSAMDT 120

Query: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180
           LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR
Sbjct: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180

Query: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVINE 240
           KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RV+TVINE
Sbjct: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTVINE 240

Query: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300
           GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA
Sbjct: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300

Query: 301 NVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF 360
           NVL HPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF
Sbjct: 301 NVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF 360

Query: 361 PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420
           PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA
Sbjct: 361 PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420

Query: 421 FTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGRWS 480
           FTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVI PE MLGTSA+VKITS+GRWS
Sbjct: 421 FTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGPEAMLGTSALVKITSVGRWS 480

Query: 481 VFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPES-CKGAVA 540
           VFGEVIETI TKHHKTT LED PIQ KVPPCSNAHETCACS+EPE C CG ES CKG VA
Sbjct: 481 VFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNAHETCACSVEPEACACGAESCCKGPVA 540

Query: 541 VDDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHVPKSEERESTIVTERKQGSAGGRLDEW 600
           +D+KDDSSRNIPL EEPKRKNL+EWLLR+RKSHVPK EERES +V+ERKQ SAGG + EW
Sbjct: 541 LDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREERESPVVSERKQASAGGSMSEW 600

Query: 601 GVVDRILVGGILISTLTIFGLLFHLGSKTLSSS 633
           GVVDRILVGGILIS  TI GLLFHLGS TLSSS
Sbjct: 601 GVVDRILVGGILISIFTIVGLLFHLGSTTLSSS 633

BLAST of HG10003393 vs. NCBI nr
Match: XP_022949069.1 (threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita moschata])

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 588/635 (92.60%), Postives = 606/635 (95.43%), Query Frame = 0

Query: 1   MEDIEDLLI--GGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPK 60
           MEDIEDLLI  GGGGAPPGYRLPI+AVGVKPKKKNKLKSNP+ADDSS+ L  TQN L PK
Sbjct: 1   MEDIEDLLIGGGGGGAPPGYRLPISAVGVKPKKKNKLKSNPDADDSSSGLFPTQNLLAPK 60

Query: 61  IPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAM 120
           IPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSAM
Sbjct: 61  IPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSAM 120

Query: 121 DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL 180
           DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL
Sbjct: 121 DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL 180

Query: 181 NRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVI 240
           NRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RV+TVI
Sbjct: 181 NRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTVI 240

Query: 241 NEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKE 300
           NEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKE
Sbjct: 241 NEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKE 300

Query: 301 IANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIIC 360
           IANVL HPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIIC
Sbjct: 301 IANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIIC 360

Query: 361 GFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVF 420
           GFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVF
Sbjct: 361 GFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVF 420

Query: 421 EAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGR 480
           EAFTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVI  ETMLGTSA+VKITS+GR
Sbjct: 421 EAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHETMLGTSALVKITSVGR 480

Query: 481 WSVFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPES-CKGA 540
           WSVFGEVIETI TKHHKTT LED PIQ KVPPCS+AHETCACS+EPE C CGPES CKG 
Sbjct: 481 WSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSDAHETCACSVEPEACACGPESCCKGP 540

Query: 541 VAVDDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHVPKSEERESTIVTERKQGSAGGRLD 600
           VA+D+KDDSSRNIPL EEPKRKNL+EWLLR+RKSH PK EERES +V+ERKQ SAGG +D
Sbjct: 541 VALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHAPKREERESPVVSERKQASAGGSMD 600

Query: 601 EWGVVDRILVGGILISTLTIFGLLFHLGSKTLSSS 633
           EWGVVDRILVGGILIS  TI GLLFHLGS TLSSS
Sbjct: 601 EWGVVDRILVGGILISIFTIVGLLFHLGSTTLSSS 635

BLAST of HG10003393 vs. NCBI nr
Match: XP_008459673.1 (PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cucumis melo])

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 590/634 (93.06%), Postives = 602/634 (94.95%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKIP 60
           MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKN  KSNP+ADDSSN+ S TQNPLVPKIP
Sbjct: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNLFKSNPSADDSSNQPSPTQNPLVPKIP 60

Query: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120
           GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT
Sbjct: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120

Query: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180
           LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR
Sbjct: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180

Query: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVINE 240
           KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV++VINE
Sbjct: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSVINE 240

Query: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300
           GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA
Sbjct: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300

Query: 301 NVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF 360
            VLSHPCVYSFLHVPVQSGSD ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDIICGF
Sbjct: 301 KVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGF 360

Query: 361 PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420
           PGETDEDFCETINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA
Sbjct: 361 PGETDEDFCETINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420

Query: 421 FTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGRWS 480
           FTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVIAPETMLGTSAM KITSIGRWS
Sbjct: 421 FTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMAKITSIGRWS 480

Query: 481 VFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPESCKGAVAV 540
           VFGEVIETISTKHHK TTLEDT  +DKV PCSN HETCACS EPE C C   SCKGAV+V
Sbjct: 481 VFGEVIETISTKHHK-TTLEDTLTRDKVSPCSNTHETCACSTEPESCACELGSCKGAVSV 540

Query: 541 DDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHV-PKSEERESTIVTERKQGSAGGRLDEW 600
            D+ DSSRNIPL EEPKRKNLIEW+LRRRKS V PK EE E+ IVTERKQ  AGGRLDEW
Sbjct: 541 GDEVDSSRNIPLPEEPKRKNLIEWVLRRRKSQVMPKREETENPIVTERKQTLAGGRLDEW 600

Query: 601 GVVDRILVGGILISTLTIFGLLFHLGSKTLSSSW 634
           GVVD ILVGGILIST TIFGLLFH+GS TLSSSW
Sbjct: 601 GVVDMILVGGILISTFTIFGLLFHVGSTTLSSSW 633

BLAST of HG10003393 vs. NCBI nr
Match: KAA0039222.1 (threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cucumis melo var. makuwa] >TYK00410.1 threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 589/634 (92.90%), Postives = 601/634 (94.79%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKIP 60
           MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKN  KSNP+ADDSSN+ S TQNPLVPKIP
Sbjct: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNLFKSNPSADDSSNQPSPTQNPLVPKIP 60

Query: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120
           GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT
Sbjct: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120

Query: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180
           LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR
Sbjct: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180

Query: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVINE 240
           KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV++VINE
Sbjct: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSVINE 240

Query: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300
           GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA
Sbjct: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300

Query: 301 NVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF 360
            VLSHPCVYSFLHVPVQSGSD ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDIICGF
Sbjct: 301 KVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGF 360

Query: 361 PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420
           PGETDEDFC+TINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA
Sbjct: 361 PGETDEDFCKTINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420

Query: 421 FTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGRWS 480
           FTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVIAPETMLGTSAM KITSIGRWS
Sbjct: 421 FTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMAKITSIGRWS 480

Query: 481 VFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPESCKGAVAV 540
           VFGEVIETISTKHHK TTLEDT  +DKV PCSN HETCACS EPE C C   SCKGAV+V
Sbjct: 481 VFGEVIETISTKHHK-TTLEDTLTRDKVSPCSNTHETCACSTEPESCACELGSCKGAVSV 540

Query: 541 DDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHV-PKSEERESTIVTERKQGSAGGRLDEW 600
            D+ DS RNIPL EEPKRKNLIEW+LRRRKS V PK EE E+ IVTERKQ  AGGRLDEW
Sbjct: 541 GDEVDSLRNIPLPEEPKRKNLIEWVLRRRKSQVMPKREETENPIVTERKQTLAGGRLDEW 600

Query: 601 GVVDRILVGGILISTLTIFGLLFHLGSKTLSSSW 634
           GVVD ILVGGILIST TIFGLLFHLGS TLSSSW
Sbjct: 601 GVVDMILVGGILISTFTIFGLLFHLGSTTLSSSW 633

BLAST of HG10003393 vs. ExPASy Swiss-Prot
Match: Q6P4Y0 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus tropicalis OX=8364 GN=cdkal1 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 2.1e-141
Identity = 253/497 (50.91%), Postives = 342/497 (68.81%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSN--PNA-DDSSNELSRTQNPLVP 60
           ++DIED+              ++A   KP  +   + N  P A   + N++   + P   
Sbjct: 9   LDDIEDI--------------VSATDPKPHDRQNARQNIVPRARKRNKNKIQEEEPPADS 68

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSA 120
            IPGTQ I+++T+GCSHN SD EYMAGQL+A+GY +++ PE ADLWL+N+CTVKSP++  
Sbjct: 69  TIPGTQKIWIRTWGCSHNNSDGEYMAGQLAAYGYSITEQPEQADLWLLNSCTVKSPAEDH 128

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
               I K + A K +V++GCVPQ     + ++G+SI+GVQQIDRVVEVVEET+KGH VRL
Sbjct: 129 FRNSIKKAQEANKKVVLSGCVPQAQPRQEYMKGLSIIGVQQIDRVVEVVEETIKGHSVRL 188

Query: 181 LNRKT-------LPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSL 240
           L +K           LDLPK+RKN  IEI+ IN GCL ACTYCKTKHARG L SY V+ L
Sbjct: 189 LGQKKDNGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGELASYPVEEL 248

Query: 241 VKRVKTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPP 300
           V R      EGV EIWL+SEDTGAYGRDIG +LP LL  +V  +P  A  MLR+GMTNPP
Sbjct: 249 VDRAAQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGA--MLRLGMTNPP 308

Query: 301 FILEHLKEIANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGM 360
           +ILEHL+E+A +L+HP VY+FLH+PVQS SD++L  M REY +++F+ VVD L E VPG+
Sbjct: 309 YILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCIADFKRVVDFLKERVPGI 368

Query: 361 QVATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKR 420
            +ATDIICGFPGETDEDF ET+ L++EYK P + I+QFYPRPGTPAA+M++VP+ + K+R
Sbjct: 369 TIATDIICGFPGETDEDFKETLKLVEEYKFPSLFINQFYPRPGTPAAKMEQVPAHVKKQR 428

Query: 421 SRELTSVFEAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAM 480
           ++EL+ +F +++PY+   G  + + +TE + D  + V H + Y QVLV      +G    
Sbjct: 429 TKELSQLFHSYSPYDHKIGEEQHVLVTEESFDSQYYVSHNRFYEQVLVPKDPAFVGKMVE 488

Query: 481 VKITSIGRWSVFGEVIE 488
           VKI   G+  + G+ ++
Sbjct: 489 VKIFEAGKHFMKGQPVQ 489

BLAST of HG10003393 vs. ExPASy Swiss-Prot
Match: Q291H5 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA19679 PE=3 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.1e-140
Identity = 259/496 (52.22%), Postives = 342/496 (68.95%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPK-- 60
           ++DIEDL+      P        +V V+ KK+ ++K   +A++       T  P + +  
Sbjct: 13  IDDIEDLISAEDVKPQERYQNKKSVTVRAKKRVQIKPETDAEEKP-----TPRPTIYESV 72

Query: 61  IPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAM 120
           IPGTQ ++VKT+GC+HN SDSEYMAGQL+A+GY LS   ++ADLWL+N+CTVK+PS+   
Sbjct: 73  IPGTQKVFVKTWGCAHNNSDSEYMAGQLAAYGYKLS-GKDEADLWLLNSCTVKNPSEDTF 132

Query: 121 DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL 180
              I    +  K +VVAGCVPQG+     L G+S++GVQQIDRVVEVVEETLKGH VRLL
Sbjct: 133 RNEIESGMSNGKHIVVAGCVPQGAPKSDYLRGLSVIGVQQIDRVVEVVEETLKGHSVRLL 192

Query: 181 NRKTL-------PALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV 240
             K +         L LPKVRKN  IEI+ IN GCL  CTYCKTKHARG L SY  + +V
Sbjct: 193 QNKKVHGRRVAGAPLSLPKVRKNPLIEIISINTGCLNQCTYCKTKHARGDLASYPPEEIV 252

Query: 241 KRVKTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPF 300
            R +    EG  EIWL+SEDTGAYGRDIG +LP LL  +V  +P     MLR+GMTNPP+
Sbjct: 253 DRARQSFAEGCCEIWLTSEDTGAYGRDIGSSLPELLWKLVEVIPEHC--MLRVGMTNPPY 312

Query: 301 ILEHLKEIANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQ 360
           ILEHL+E+A VL HP VY+FLHVPVQSGSD++L  M REY   +F  VVD L E VPG+ 
Sbjct: 313 ILEHLEEVAKVLQHPRVYAFLHVPVQSGSDSVLGEMKREYCRKDFEHVVDFLRERVPGVT 372

Query: 361 VATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRS 420
           +ATDIICGFP ET+EDF ET+ L   Y+ P + I+QF+PRPGTPAA+M+++P+ +VKKR+
Sbjct: 373 IATDIICGFPTETEEDFEETMTLCGRYRFPSLFINQFFPRPGTPAAKMERIPANLVKKRT 432

Query: 421 RELTSVFEAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMV 480
           + LT +F ++ PY    G +  + +TEI+ D +H VGH K Y QVL+   + +LGT   V
Sbjct: 433 KRLTDLFYSYEPYAQRVGEMYTVLVTEISHDKLHYVGHNKSYEQVLLPMRDNLLGTRVHV 492

Query: 481 KITSIGRWSVFGEVIE 488
           +ITS+ ++S+ GE+++
Sbjct: 493 RITSVSKFSMVGEILD 500

BLAST of HG10003393 vs. ExPASy Swiss-Prot
Match: Q6NS26 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus laevis OX=8355 GN=cdkal1 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.4e-140
Identity = 251/494 (50.81%), Postives = 339/494 (68.62%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKIP 60
           ++DIED++      P   +     +  + +K+NK           N +   + P    IP
Sbjct: 9   LDDIEDMVSATDPKPHDRQSARKNIVPRARKRNK-----------NNIQEEEPPADSTIP 68

Query: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120
           GTQ I+++T+GCSHN SD EYMAGQL+A+GY +++ PE ADLWL+N+CTVKSP++     
Sbjct: 69  GTQKIWIRTWGCSHNNSDGEYMAGQLAAYGYSITEQPEKADLWLLNSCTVKSPAEDHFRN 128

Query: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180
            I K + A K +VV+GCVPQ       ++G+SI+GVQQIDRVVEVVEET+KGH VRLL +
Sbjct: 129 SIKKAQEANKKVVVSGCVPQAQPRQDYMKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQ 188

Query: 181 KT-------LPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKR 240
           K           LDLPK+RKN  IEI+ IN GCL ACTYCKTKHARG L SY V+ LV R
Sbjct: 189 KKDNGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGELASYPVEELVDR 248

Query: 241 VKTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFIL 300
                 EGV EIWL+SEDTGAYGRDIG +LP LL  +V  +P  A  MLR+GMTNPP+IL
Sbjct: 249 ATQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGA--MLRLGMTNPPYIL 308

Query: 301 EHLKEIANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVA 360
           EHL+E+A +L+HP VY+FLH+PVQS SD++L  M REY +++F+ VVD L E VPG+ +A
Sbjct: 309 EHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCIADFKRVVDFLKERVPGITIA 368

Query: 361 TDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRE 420
           TDIICGFPGETDEDF ET+ L++EYK P + I+QFYPRPGTPAA+M++V + + K+R++E
Sbjct: 369 TDIICGFPGETDEDFKETLKLVEEYKFPSLFINQFYPRPGTPAAKMEQVLAHVKKRRTKE 428

Query: 421 LTSVFEAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKI 480
           L+ +F ++ PY+   G+ +++ +TE + D  + V H + Y QVLV      +G    VKI
Sbjct: 429 LSQLFHSYDPYDHKIGQKQQVLVTEESFDSQYYVAHNRFYEQVLVPKDPDFMGKMVEVKI 488

Query: 481 TSIGRWSVFGEVIE 488
              G+  + G+ ++
Sbjct: 489 FEAGKHFMKGQPVQ 489

BLAST of HG10003393 vs. ExPASy Swiss-Prot
Match: Q5VV42 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Homo sapiens OX=9606 GN=CDKAL1 PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.5e-139
Identity = 252/493 (51.12%), Postives = 336/493 (68.15%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKIP 60
           ++DIED++      P         V  K +++N  K     ++S         P    IP
Sbjct: 10  LDDIEDIVSQEDSKPQDRHFVRKDVVPKVRRRNTQKYLQEEENSP--------PSDSTIP 69

Query: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120
           G Q I+++T+GCSHN SD EYMAGQL+A+GY +++N  DADLWL+N+CTVK+P++     
Sbjct: 70  GIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRN 129

Query: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180
            I K +   K +V+AGCVPQ       L+G+SI+GVQQIDRVVEVVEET+KGH VRLL +
Sbjct: 130 SIKKAQEENKKIVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQ 189

Query: 181 KT-------LPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKR 240
           K           LDLPK+RKN  IEI+ IN GCL ACTYCKTKHARG+L SY +D LV R
Sbjct: 190 KKDNGRRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVDR 249

Query: 241 VKTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFIL 300
            K    EGV EIWL+SEDTGAYGRDIG NLP LL  +V  +P  A  MLR+GMTNPP+IL
Sbjct: 250 AKQSFQEGVCEIWLTSEDTGAYGRDIGTNLPTLLWKLVEVIPEGA--MLRLGMTNPPYIL 309

Query: 301 EHLKEIANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVA 360
           EHL+E+A +L+HP VY+FLH+PVQS SD++L  M REY V++F+ VVD L E VPG+ +A
Sbjct: 310 EHLEEMAKILNHPRVYAFLHIPVQSASDSVLMEMKREYCVADFKRVVDFLKEKVPGITIA 369

Query: 361 TDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRE 420
           TDIICGFPGETD+DF ET+ L++EYK P + I+QFYPRPGTPAA+M++VP+ + K+R+++
Sbjct: 370 TDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKMEQVPAQVKKQRTKD 429

Query: 421 LTSVFEAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKI 480
           L+ VF +++PY+   G  +++ +TE + D    V H + Y QVLV      +G    V I
Sbjct: 430 LSRVFHSYSPYDHKIGERQQVLVTEESFDSKFYVAHNQFYEQVLVPKNPAFMGKMVEVDI 489

Query: 481 TSIGRWSVFGEVI 487
              G+  + G+ +
Sbjct: 490 YESGKHFMKGQPV 492

BLAST of HG10003393 vs. ExPASy Swiss-Prot
Match: Q7K4W1 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila melanogaster OX=7227 GN=CG6550 PE=2 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.6e-139
Identity = 259/497 (52.11%), Postives = 341/497 (68.61%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPK-- 60
           ++DIEDL+      P         V V+ KK+++++        S E      P + +  
Sbjct: 13  VDDIEDLISADDVKPRERYENKKTVTVRAKKRSQIRL------ESQEEEEKPKPTIHESV 72

Query: 61  IPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAM 120
           IPGTQ ++VKT+GC+HN SDSEYMAGQL+A+GY LS   E+ADLWL+N+CTVK+PS+   
Sbjct: 73  IPGTQKVFVKTWGCAHNNSDSEYMAGQLAAYGYRLS-GKEEADLWLLNSCTVKNPSEDTF 132

Query: 121 DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL 180
              I       K +VVAGCVPQG+     L G+S++GVQQIDRVVEVVEETLKGH V+LL
Sbjct: 133 RNEIESGMRNGKHVVVAGCVPQGAPKSDYLNGLSVIGVQQIDRVVEVVEETLKGHSVQLL 192

Query: 181 -NRKTL-------PALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSL 240
            N+K +         L LPKVRKN  IEI+ IN GCL  CTYCKTKHARG L SY  + +
Sbjct: 193 QNKKKVHGRRVAGAPLSLPKVRKNPLIEIISINSGCLNQCTYCKTKHARGDLASYPPEEV 252

Query: 241 VKRVKTVINEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPP 300
           V+R +    EG  EIWL+SEDTGAYGRDIG +LP LL  +V  +P     MLR+GMTNPP
Sbjct: 253 VERARQSFAEGCCEIWLTSEDTGAYGRDIGSSLPELLWQLVEVIPEHC--MLRVGMTNPP 312

Query: 301 FILEHLKEIANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGM 360
           +ILEHL+E+ANVL HP VYSFLHVPVQSGSD++L  M REY   +F  VVD L E VPG+
Sbjct: 313 YILEHLEEVANVLQHPRVYSFLHVPVQSGSDSVLGEMKREYCRQDFEHVVDFLRERVPGV 372

Query: 361 QVATDIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKR 420
            +ATDIICGFP ET++DF ET+ L  +Y+ P + I+QF+PRPGTPAA+M ++P+ +VKKR
Sbjct: 373 TIATDIICGFPTETEDDFEETMTLCAKYRFPSLFINQFFPRPGTPAAKMDRIPANLVKKR 432

Query: 421 SRELTSVFEAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAM 480
           ++ LT +F ++ PY    G +  + +TE++ D +H VGH K Y QVL+   + +LGT   
Sbjct: 433 TKRLTDLFYSYEPYADRVGEIYTVLVTEVSHDKLHYVGHNKSYEQVLLPMRDNLLGTRVH 492

Query: 481 VKITSIGRWSVFGEVIE 488
           V+ITS  ++S+ GE+++
Sbjct: 493 VRITSASKFSMVGEILD 500

BLAST of HG10003393 vs. ExPASy TrEMBL
Match: A0A6J1GBS1 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita moschata OX=3662 GN=LOC111452531 PE=3 SV=1)

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 588/635 (92.60%), Postives = 606/635 (95.43%), Query Frame = 0

Query: 1   MEDIEDLLI--GGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPK 60
           MEDIEDLLI  GGGGAPPGYRLPI+AVGVKPKKKNKLKSNP+ADDSS+ L  TQN L PK
Sbjct: 1   MEDIEDLLIGGGGGGAPPGYRLPISAVGVKPKKKNKLKSNPDADDSSSGLFPTQNLLAPK 60

Query: 61  IPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAM 120
           IPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSAM
Sbjct: 61  IPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSAM 120

Query: 121 DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL 180
           DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL
Sbjct: 121 DTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL 180

Query: 181 NRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVI 240
           NRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RV+TVI
Sbjct: 181 NRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTVI 240

Query: 241 NEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKE 300
           NEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKE
Sbjct: 241 NEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKE 300

Query: 301 IANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIIC 360
           IANVL HPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIIC
Sbjct: 301 IANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIIC 360

Query: 361 GFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVF 420
           GFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVF
Sbjct: 361 GFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVF 420

Query: 421 EAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGR 480
           EAFTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVI  ETMLGTSA+VKITS+GR
Sbjct: 421 EAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHETMLGTSALVKITSVGR 480

Query: 481 WSVFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPES-CKGA 540
           WSVFGEVIETI TKHHKTT LED PIQ KVPPCS+AHETCACS+EPE C CGPES CKG 
Sbjct: 481 WSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSDAHETCACSVEPEACACGPESCCKGP 540

Query: 541 VAVDDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHVPKSEERESTIVTERKQGSAGGRLD 600
           VA+D+KDDSSRNIPL EEPKRKNL+EWLLR+RKSH PK EERES +V+ERKQ SAGG +D
Sbjct: 541 VALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHAPKREERESPVVSERKQASAGGSMD 600

Query: 601 EWGVVDRILVGGILISTLTIFGLLFHLGSKTLSSS 633
           EWGVVDRILVGGILIS  TI GLLFHLGS TLSSS
Sbjct: 601 EWGVVDRILVGGILISIFTIVGLLFHLGSTTLSSS 635

BLAST of HG10003393 vs. ExPASy TrEMBL
Match: A0A1S3CA89 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo OX=3656 GN=LOC103498722 PE=3 SV=1)

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 590/634 (93.06%), Postives = 602/634 (94.95%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKIP 60
           MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKN  KSNP+ADDSSN+ S TQNPLVPKIP
Sbjct: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNLFKSNPSADDSSNQPSPTQNPLVPKIP 60

Query: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120
           GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT
Sbjct: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120

Query: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180
           LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR
Sbjct: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180

Query: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVINE 240
           KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV++VINE
Sbjct: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSVINE 240

Query: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300
           GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA
Sbjct: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300

Query: 301 NVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF 360
            VLSHPCVYSFLHVPVQSGSD ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDIICGF
Sbjct: 301 KVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGF 360

Query: 361 PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420
           PGETDEDFCETINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA
Sbjct: 361 PGETDEDFCETINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420

Query: 421 FTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGRWS 480
           FTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVIAPETMLGTSAM KITSIGRWS
Sbjct: 421 FTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMAKITSIGRWS 480

Query: 481 VFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPESCKGAVAV 540
           VFGEVIETISTKHHK TTLEDT  +DKV PCSN HETCACS EPE C C   SCKGAV+V
Sbjct: 481 VFGEVIETISTKHHK-TTLEDTLTRDKVSPCSNTHETCACSTEPESCACELGSCKGAVSV 540

Query: 541 DDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHV-PKSEERESTIVTERKQGSAGGRLDEW 600
            D+ DSSRNIPL EEPKRKNLIEW+LRRRKS V PK EE E+ IVTERKQ  AGGRLDEW
Sbjct: 541 GDEVDSSRNIPLPEEPKRKNLIEWVLRRRKSQVMPKREETENPIVTERKQTLAGGRLDEW 600

Query: 601 GVVDRILVGGILISTLTIFGLLFHLGSKTLSSSW 634
           GVVD ILVGGILIST TIFGLLFH+GS TLSSSW
Sbjct: 601 GVVDMILVGGILISTFTIFGLLFHVGSTTLSSSW 633

BLAST of HG10003393 vs. ExPASy TrEMBL
Match: A0A5D3BN67 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00090 PE=3 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 589/634 (92.90%), Postives = 601/634 (94.79%), Query Frame = 0

Query: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKIP 60
           MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKN  KSNP+ADDSSN+ S TQNPLVPKIP
Sbjct: 1   MEDIEDLLIGGGGAPPGYRLPITAVGVKPKKKNLFKSNPSADDSSNQPSPTQNPLVPKIP 60

Query: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120
           GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT
Sbjct: 61  GTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMDT 120

Query: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180
           LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR
Sbjct: 121 LITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNR 180

Query: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVINE 240
           KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRV++VINE
Sbjct: 181 KTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVRSVINE 240

Query: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300
           GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA
Sbjct: 241 GVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEIA 300

Query: 301 NVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICGF 360
            VLSHPCVYSFLHVPVQSGSD ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDIICGF
Sbjct: 301 KVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGF 360

Query: 361 PGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420
           PGETDEDFC+TINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA
Sbjct: 361 PGETDEDFCKTINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFEA 420

Query: 421 FTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGRWS 480
           FTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVIAPETMLGTSAM KITSIGRWS
Sbjct: 421 FTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSAMAKITSIGRWS 480

Query: 481 VFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPESCKGAVAV 540
           VFGEVIETISTKHHK TTLEDT  +DKV PCSN HETCACS EPE C C   SCKGAV+V
Sbjct: 481 VFGEVIETISTKHHK-TTLEDTLTRDKVSPCSNTHETCACSTEPESCACELGSCKGAVSV 540

Query: 541 DDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHV-PKSEERESTIVTERKQGSAGGRLDEW 600
            D+ DS RNIPL EEPKRKNLIEW+LRRRKS V PK EE E+ IVTERKQ  AGGRLDEW
Sbjct: 541 GDEVDSLRNIPLPEEPKRKNLIEWVLRRRKSQVMPKREETENPIVTERKQTLAGGRLDEW 600

Query: 601 GVVDRILVGGILISTLTIFGLLFHLGSKTLSSSW 634
           GVVD ILVGGILIST TIFGLLFHLGS TLSSSW
Sbjct: 601 GVVDMILVGGILISTFTIFGLLFHLGSTTLSSSW 633

BLAST of HG10003393 vs. ExPASy TrEMBL
Match: A0A0A0KSZ4 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis sativus OX=3659 GN=Csa_5G652200 PE=3 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 588/635 (92.60%), Postives = 601/635 (94.65%), Query Frame = 0

Query: 1   MEDIEDLLI-GGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVPKI 60
           MEDIEDLLI GGGGAPPGYRLPITAVGVKPKKKN  KSN +ADDSSNE S  QNPLVPKI
Sbjct: 1   MEDIEDLLIGGGGGAPPGYRLPITAVGVKPKKKNMFKSNASADDSSNEPSPIQNPLVPKI 60

Query: 61  PGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMD 120
           PGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMD
Sbjct: 61  PGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSAMD 120

Query: 121 TLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLN 180
           TLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLN
Sbjct: 121 TLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLN 180

Query: 181 RKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVIN 240
           RKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV+RV++VIN
Sbjct: 181 RKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVRRVRSVIN 240

Query: 241 EGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEI 300
           EGVREIWLSSEDTGAYGRDIGVNLPILLNAIV+ELP DASTMLRIGMTNPPFILEHLKEI
Sbjct: 241 EGVREIWLSSEDTGAYGRDIGVNLPILLNAIVSELPSDASTMLRIGMTNPPFILEHLKEI 300

Query: 301 ANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDIICG 360
           A VLSHPCVYSFLHVPVQSGSD ILSAMNREYTVSEFRTVVDTLTELVPGMQ+ATDIICG
Sbjct: 301 AKVLSHPCVYSFLHVPVQSGSDAILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICG 360

Query: 361 FPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFE 420
           FPGETDEDFCETINLIKEY LPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFE
Sbjct: 361 FPGETDEDFCETINLIKEYNLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSVFE 420

Query: 421 AFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIGRW 480
           AFTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVIAPETMLGTSA VKITSIGRW
Sbjct: 421 AFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIAPETMLGTSATVKITSIGRW 480

Query: 481 SVFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPESCKGAVA 540
           SVFGEVIE ISTKHHKTTTLEDT  QDKV PCSN HETCACS EPE C CG ESCKGAVA
Sbjct: 481 SVFGEVIEIISTKHHKTTTLEDTLTQDKVSPCSNTHETCACSTEPESCACGLESCKGAVA 540

Query: 541 VDDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHV-PKSEERESTIVTERKQGSAGGRLDE 600
           V D+ +SSRN+P  EEPKRKNLIEW+LRRRKSHV PK EE E+ IVTERKQ  AGGRLDE
Sbjct: 541 VGDEVNSSRNVPSPEEPKRKNLIEWVLRRRKSHVLPKREETENPIVTERKQTLAGGRLDE 600

Query: 601 WGVVDRILVGGILISTLTIFGLLFHLGSKTLSSSW 634
           WGVVD+ILVGGILIST TIFGLLFHLGS T SSSW
Sbjct: 601 WGVVDKILVGGILISTFTIFGLLFHLGSTTFSSSW 635

BLAST of HG10003393 vs. ExPASy TrEMBL
Match: A0A6J1KEZ5 (Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita maxima OX=3661 GN=LOC111492654 PE=3 SV=1)

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 583/636 (91.67%), Postives = 601/636 (94.50%), Query Frame = 0

Query: 1   MEDIEDLLI---GGGGAPPGYRLPITAVGVKPKKKNKLKSNPNADDSSNELSRTQNPLVP 60
           MEDIEDLLI   GGGGAPPGYRLPI+AVGVK KKKNKLK NP+ADDSS+ L  TQN L P
Sbjct: 1   MEDIEDLLIGGGGGGGAPPGYRLPISAVGVKSKKKNKLKPNPDADDSSSGLFPTQNLLAP 60

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSA 120
           KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLS+NPE+ADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSENPEEADLWLINTCTVKSPSQSA 120

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
           MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL
Sbjct: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180

Query: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTV 240
           LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RV+TV
Sbjct: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVNRVRTV 240

Query: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300
           INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK
Sbjct: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300

Query: 301 EIANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360
           EIANVL HPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII
Sbjct: 301 EIANVLCHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360

Query: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420
           CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV
Sbjct: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420

Query: 421 FEAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIG 480
           FEAFTPYNGMEGRVERIWITEIAADG+HLVGHTKGYIQVLVI  E MLGTSA+VKITS+G
Sbjct: 421 FEAFTPYNGMEGRVERIWITEIAADGIHLVGHTKGYIQVLVIGHEAMLGTSALVKITSVG 480

Query: 481 RWSVFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPES-CKG 540
           RWSVFGEVIETI TKHHKTT LED PIQ KVPPCSN+HETCACS+EPE C CGPES CKG
Sbjct: 481 RWSVFGEVIETIVTKHHKTTALEDMPIQAKVPPCSNSHETCACSVEPEACACGPESCCKG 540

Query: 541 AVAVDDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHVPKSEERESTIVTERKQGSAGGRL 600
            VA+D+KDDSSRNIPL EEPKRKNL+EWLLR+RKSHVPK EE ES +V+ RKQ SAGG +
Sbjct: 541 PVALDEKDDSSRNIPLPEEPKRKNLVEWLLRKRKSHVPKREEGESPVVSGRKQASAGGSM 600

Query: 601 DEWGVVDRILVGGILISTLTIFGLLFHLGSKTLSSS 633
            EWGVVDRILVGGILIS  TI GLLFHLGS TLSSS
Sbjct: 601 SEWGVVDRILVGGILISIFTIVGLLFHLGSTTLSSS 636

BLAST of HG10003393 vs. TAIR 10
Match: AT1G72090.1 (Methylthiotransferase )

HSP 1 Score: 854.4 bits (2206), Expect = 6.0e-248
Identity = 444/632 (70.25%), Postives = 504/632 (79.75%), Query Frame = 0

Query: 1   MEDIEDLLIGG-GGAPPGYRLPITAVGVKPK--KKNKLKSNPNADDSSNELSRTQNPLVP 60
           MEDIEDLL GG GGAPPG+RLP+ AVG+ PK  K  ++ S P+   +SN  S    P   
Sbjct: 1   MEDIEDLLAGGVGGAPPGFRLPLNAVGINPKTNKSKRISSKPDQITASNRDSLA--PPSM 60

Query: 61  KIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYLLSDNPEDADLWLINTCTVKSPSQSA 120
           KIPGTQTIY+KTFGCSHNQSDSEYMAGQLSAFGY L++ PE+ADLWLINTCTVKSPSQSA
Sbjct: 61  KIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALTEVPEEADLWLINTCTVKSPSQSA 120

Query: 121 MDTLITKCKNAKKPLVVAGCVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL 180
           M TLIT+ ++ KKPLV+AGCVPQGSRDLKELEGVS+VGVQQIDRVVE+VEETLKGHEVRL
Sbjct: 121 MSTLITRGRSGKKPLVIAGCVPQGSRDLKELEGVSVVGVQQIDRVVEIVEETLKGHEVRL 180

Query: 181 LNRKTLPALDLPKVRKNKFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTV 240
           L RKTLPALDLPKVR+N FIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV+RV+TV
Sbjct: 181 LTRKTLPALDLPKVRRNNFIEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVERVRTV 240

Query: 241 INEGVREIWLSSEDTGAYGRDIGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLK 300
           I+EGV+EIWLSSEDTGAYGRDIGVNLPILLNAIV ELP D STMLRIGMTNPPFILEHLK
Sbjct: 241 ISEGVKEIWLSSEDTGAYGRDIGVNLPILLNAIVKELPSDQSTMLRIGMTNPPFILEHLK 300

Query: 301 EIANVLSHPCVYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVATDII 360
           EIA VL HPCVY+FLHVPVQSGSD++L+AMNREYT SEFRTVVDTLTELVPGMQ+ATDII
Sbjct: 301 EIAAVLRHPCVYTFLHVPVQSGSDSVLTAMNREYTASEFRTVVDTLTELVPGMQIATDII 360

Query: 361 CGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAARMKKVPSAIVKKRSRELTSV 420
           CGFPGETDEDF +T+ LIK+YK PQVHISQFYPRPGTPAA+MKKV S IVK+RSRELTSV
Sbjct: 361 CGFPGETDEDFSQTVELIKDYKFPQVHISQFYPRPGTPAAKMKKVQSKIVKQRSRELTSV 420

Query: 421 FEAFTPYNGMEGRVERIWITEIAADGVHLVGHTKGYIQVLVIAPETMLGTSAMVKITSIG 480
           FEAF PY GME R ERIWITE+A DG+HLVGHTKGY+QVLV  PE+MLGTSAM +ITS+G
Sbjct: 421 FEAFAPYTGMECREERIWITEVATDGIHLVGHTKGYVQVLVTGPESMLGTSAMARITSVG 480

Query: 481 RWSVFGEVIETISTKHHKTTTLEDTPIQDKVPPCSNAHETCACSMEPEPCGCGPESCKGA 540
           RWSVFGEVIET S+ + +T + E+T +     PCS+   TC      E C C  ESC G 
Sbjct: 481 RWSVFGEVIETFSSANRETKSREETKL-----PCSSNVSTC------ETCTCSAESC-GE 540

Query: 541 VAVDDKDDSSRNIPLAEEPKRKNLIEWLLRRRKSHVPKSEERESTIVTERKQGSAGGRLD 600
               +  + S NI   ++ K K+              K EE+E   V        G  + 
Sbjct: 541 ERSGEACNISGNISGQDDNKGKS--------------KKEEKEVQEVV-----VPGSSVA 599

Query: 601 EWGVVDRILVGGILISTLTIFGLLFHLGSKTL 630
            WG +D+ LV G+ +S++TI  LL  +  + L
Sbjct: 601 NWGFIDKALVCGVFVSSVTILVLLISIACRVL 599

BLAST of HG10003393 vs. TAIR 10
Match: AT4G36390.1 (Methylthiotransferase )

HSP 1 Score: 124.8 bits (312), Expect = 2.5e-28
Identity = 110/474 (23.21%), Postives = 201/474 (42.41%), Query Frame = 0

Query: 49  SRTQNPLVPKIPGTQTIYVKTFGCSHNQSDSEYMAGQLSAFGYL-LSDNPEDADLWLINT 108
           + +++ L   I     IY +T+GC  N +D E +   +   GY  +  +PE A++  +NT
Sbjct: 115 TESESTLDSDIASKGRIYHETYGCQMNINDMEIVLAIMKNSGYKEVVTDPESAEVIFVNT 174

Query: 109 CTVKSPSQS----------------AMDTLITKCKNAKKP-LVVAGCVPQGSRDLKELEG 168
           C ++  ++                  ++    + K+ K P +VV GC+ +  +D K L+ 
Sbjct: 175 CAIRENAEQRVWQRLNYFWFLKREWKVNAATGRAKSLKPPKVVVLGCMAERLKD-KILDS 234

Query: 169 VSIVGV-------QQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFIEILPIN 228
             +V V       + + R++E V+   KG    L   +T   +   ++ +N     + + 
Sbjct: 235 DKMVDVVCGPDAYRDLPRLLEEVDYGQKGINTLLSLEETYADISPVRISENSITAFVSVM 294

Query: 229 VGCLGACTYCKTKHARGHLGSYTVDSLVKRVKTVINEGVREIWLSSEDTGAYGRD----- 288
            GC   C +C     RG   S  V+S+++ V  +   GV+E+ L  ++  +Y  D     
Sbjct: 295 RGCNNMCAFCIVPFTRGRERSRPVESIIREVGELWESGVKEVTLLGQNVNSYNDDSADRE 354

Query: 289 -------------------IGVNLPILLNAIVAELPPDASTMLRIGMTNPPFILEHLKEI 348
                              +G+    LL+ +  E P      +R   T+P     H K+ 
Sbjct: 355 SGANWEYSEGFSSRCKVKNMGLRFADLLDRLSVEFPE-----MRFRFTSP-----HPKDY 414

Query: 349 ANVLSHPC-----VYSFLHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQVAT 408
            + L +       + + +H+P QSG+  IL  M R YT   +  +V  +  ++P + + +
Sbjct: 415 PDELLYLMRDRHNICNLIHLPAQSGNSRILEQMRRGYTREAYLDLVKKIRSIIPDVAITS 474

Query: 409 DIICGFPGETDEDFCETINLIKEYKLPQVHISQFYPRPGTPAAR--MKKVPSAIVKKRSR 462
           D I GF GET+E+  ET++L++       ++  +  R  T A R     VP  + ++R  
Sbjct: 475 DFITGFCGETEEEHQETLSLVRAVGYDMAYMFAYSMREKTHAHRNYTDDVPEEVKQRRLT 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890156.10.0e+0096.05threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Benincasa hisp... [more]
XP_023524910.10.0e+0092.73threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita pepo subsp. pep... [more]
XP_022949069.10.0e+0092.60threonylcarbamoyladenosine tRNA methylthiotransferase [Cucurbita moschata][more]
XP_008459673.10.0e+0093.06PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cuc... [more]
KAA0039222.10.0e+0092.90threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1 [Cucumis melo v... [more]
Match NameE-valueIdentityDescription
Q6P4Y02.1e-14150.91Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus tropicalis OX=8... [more]
Q291H51.1e-14052.22Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila pseudoobscur... [more]
Q6NS261.4e-14050.81Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Xenopus laevis OX=8355 ... [more]
Q5VV421.5e-13951.12Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Homo sapiens OX=9606 GN... [more]
Q7K4W12.6e-13952.11Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Drosophila melanogaster... [more]
Match NameE-valueIdentityDescription
A0A6J1GBS10.0e+0092.60Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita moschata OX=3... [more]
A0A1S3CA890.0e+0093.06Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo OX=3656 GN... [more]
A0A5D3BN670.0e+0092.90Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis melo var. makuw... [more]
A0A0A0KSZ40.0e+0092.60Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucumis sativus OX=3659... [more]
A0A6J1KEZ50.0e+0091.67Threonylcarbamoyladenosine tRNA methylthiotransferase OS=Cucurbita maxima OX=366... [more]
Match NameE-valueIdentityDescription
AT1G72090.16.0e-24870.25Methylthiotransferase [more]
AT4G36390.12.5e-2823.21Methylthiotransferase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 196..417
e-value: 4.9E-41
score: 152.3
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 203..375
e-value: 9.8E-20
score: 71.6
IPR007197Radical SAMSFLDSFLDS00029Radical_SAMcoord: 138..408
e-value: 7.7E-28
score: 92.0
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 192..425
score: 28.191652
IPR038135Methylthiotransferase, N-terminal domain superfamilyGENE3D3.40.50.12160coord: 64..183
e-value: 2.3E-28
score: 100.4
IPR006466MiaB-like tRNA modifying enzyme, archaeal-typeTIGRFAMTIGR01578TIGR01578coord: 65..486
e-value: 2.1E-136
score: 453.0
IPR005839MethylthiotransferaseTIGRFAMTIGR00089TIGR00089coord: 64..481
e-value: 2.3E-98
score: 327.8
IPR013848Methylthiotransferase, N-terminalPFAMPF00919UPF0004coord: 65..143
e-value: 8.1E-20
score: 70.7
IPR013848Methylthiotransferase, N-terminalPROSITEPS51449MTTASE_Ncoord: 63..171
score: 29.050375
IPR023404Radical SAM, alpha/beta horseshoeGENE3D3.80.30.20tm_1862 like domaincoord: 194..424
e-value: 7.7E-69
score: 233.7
NoneNo IPR availableSFLDSFLDG01082B12-binding_domain_containingcoord: 138..408
e-value: 7.7E-28
score: 92.0
NoneNo IPR availablePIRSRPIRSR000167-1PIRSR000167-1coord: 316..400
e-value: 2.1E-6
score: 25.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 29..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..57
NoneNo IPR availablePANTHERPTHR11918RADICAL SAM PROTEINScoord: 1..531
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 200..405
e-value: 1.79832E-6
score: 46.943
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 186..420
IPR020612Methylthiotransferase, conserved sitePROSITEPS01278MTTASE_RADICALcoord: 200..220
IPR003616Post-SET domainPROSITEPS50868POST_SETcoord: 523..539
score: 8.380535
IPR002792TRAM domainPROSITEPS50926TRAMcoord: 425..487
score: 10.158624

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003393.1HG10003393.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035600 tRNA methylthiolation
biological_process GO:0006400 tRNA modification
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0035598 N6-threonylcarbomyladenosine methylthiotransferase activity
molecular_function GO:0061712 tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0035596 methylthiotransferase activity
molecular_function GO:0016740 transferase activity