HG10003386 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10003386 vs. NCBI nr
Match: XP_038891005.1 (protein NRT1/ PTR FAMILY 5.10-like [Benincasa hispida])

HSP 1 Score: 941.0 bits (2431), Expect = 4.4e-270
Identity = 486/542 (89.67%), Postives = 506/542 (93.36%), Query Frame = 0

Query: 1   MERRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60
           ME  TP L QTV+GAVDFKGQPV R SSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 1   MEPGTPLLRQTVEGAVDFKGQPVLRFSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 61  SPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSA 120
            PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLS LSFSA
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 121 LLSPTISP---QFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFN 180
           +L  T SP   QFQLL FFVSLYLIGIGQGGHKPCVQAFGADQFDALDP+EAKSKSSFFN
Sbjct: 121 MLPTTTSPQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPQEAKSKSSFFN 180

Query: 181 WWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDK 240
           WWFFGVCAGTFVAIL+VTYTEENLSWSLGFGIPCIMM+IASFLF+ GT TYRY+IKTYDK
Sbjct: 181 WWFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLLGTNTYRYTIKTYDK 240

Query: 241 TPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNKAC 300
           TPFLRIGRVFVSAIRNWRASSTVIFDEE N RDLS QNAGQF           RFLNKAC
Sbjct: 241 TPFLRIGRVFVSAIRNWRASSTVIFDEEGNGRDLSQQNAGQF-----------RFLNKAC 300

Query: 301 IVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRSIF 360
           IVPKDSNKCGMMCSVSEVEEAKAVLRIFP+WITVLVFAIVF+QDSTFFTKQGATMDRSI 
Sbjct: 301 IVPKDSNKCGMMCSVSEVEEAKAVLRIFPIWITVLVFAIVFSQDSTFFTKQGATMDRSIL 360

Query: 361 SGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISM 420
           SGFIIPAAALD+FVPLSIVI +TIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISM
Sbjct: 361 SGFIIPAAALDAFVPLSIVIFVTIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISM 420

Query: 421 LVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQV 480
           LVATMVERKRLR+A++HGLVD+PD+TIP+SFWWLVPQYTLFGLAE FTLVGLQEFFYDQV
Sbjct: 421 LVATMVERKRLRIADKHGLVDKPDITIPISFWWLVPQYTLFGLAEVFTLVGLQEFFYDQV 480

Query: 481 PTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYLLL 540
           PTDLKSMGLAFYTSVLGMGSILSSLLVSVIDE+TGGS + NSWFSN+LNKAHLDYFYLLL
Sbjct: 481 PTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEVTGGS-EQNSWFSNNLNKAHLDYFYLLL 530

BLAST of HG10003386 vs. NCBI nr
Match: XP_008459680.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo])

HSP 1 Score: 911.8 bits (2355), Expect = 2.9e-261
Identity = 477/540 (88.33%), Postives = 496/540 (91.85%), Query Frame = 0

Query: 1   MERRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60
           ME+ TP LSQTV+ AVDFKGQP  R  SGGWRSASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 1   MEQPTPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 61  SPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSA 120
            PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLS LSFSA
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 121 LLSPTIS--PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFNW 180
           +L  T S   QFQL+FFFVSLYLIGIGQGGHKPCVQAFGADQFDAL P+EAKSKSSFFNW
Sbjct: 121 MLPTTSSQNSQFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 180

Query: 181 WFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDKT 240
           WFFGVCAGTFVAIL+VTYTEENLSWSLGFGIPCIMM+IASFLF+FGT TYRYSIK Y KT
Sbjct: 181 WFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGTNTYRYSIKMYAKT 240

Query: 241 PFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNKACI 300
           PFLRIGRVFVSAIRN RASSTV FDEE +  DLS QN GQF+           FLNKACI
Sbjct: 241 PFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFS-----------FLNKACI 300

Query: 301 VPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRSIFS 360
           VPKDSNK G+MCS SEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGAT+DRSI S
Sbjct: 301 VPKDSNKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSILS 360

Query: 361 GFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML 420
           GFIIPAAALDSFVPLSIVI ITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML
Sbjct: 361 GFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML 420

Query: 421 VATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQVP 480
           VATMVERKRLR+A+EHGLVDRPD+TIPMSFWWLVPQYTLFGLAE FTLVGLQEFFYDQVP
Sbjct: 421 VATMVERKRLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVP 480

Query: 481 TDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYLLLS 539
           TDLKSMGLAFYTSVLGMGSILSSLLVS+IDE+TGGS + NSWFSN+LNKAHLDYFYLLLS
Sbjct: 481 TDLKSMGLAFYTSVLGMGSILSSLLVSIIDEVTGGS-EQNSWFSNNLNKAHLDYFYLLLS 528

BLAST of HG10003386 vs. NCBI nr
Match: XP_011656125.1 (protein NRT1/ PTR FAMILY 5.10 isoform X1 [Cucumis sativus] >KAE8649020.1 hypothetical protein Csa_009070 [Cucumis sativus])

HSP 1 Score: 904.8 bits (2337), Expect = 3.5e-259
Identity = 474/541 (87.62%), Postives = 494/541 (91.31%), Query Frame = 0

Query: 1   MERRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60
           ME  TP LSQTV+GAVDFKG+PV R  SGGWRSASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 1   MELPTPLLSQTVEGAVDFKGRPVLRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 61  SPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSA 120
            PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLS LSFSA
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 121 LLSPTIS--PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFNW 180
           +L  T S   QFQLL FFVSLYLIGIGQGGHKPCVQAFGADQFDAL P+EAKSKSSFFNW
Sbjct: 121 MLPTTSSQNSQFQLLLFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 180

Query: 181 WFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDKT 240
           WFFG+CAGTFVAIL+VTYTEENLSWSLGFGIPCIMM+IASFLF+FGT TYRYSIK Y +T
Sbjct: 181 WFFGICAGTFVAILLVTYTEENLSWSLGFGIPCIMMIIASFLFLFGTNTYRYSIKIYAQT 240

Query: 241 PFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNKACI 300
           PFLRIGRVFVSAIRN RASSTVIFDEE +  DLS QNAGQF           RFLNKACI
Sbjct: 241 PFLRIGRVFVSAIRNCRASSTVIFDEEGDGPDLSQQNAGQF-----------RFLNKACI 300

Query: 301 VPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRSIFS 360
           VPKDS+K G+MCS SEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGAT+DRSI S
Sbjct: 301 VPKDSDKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSIRS 360

Query: 361 GFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML 420
           GFIIPAAALDSFVPLSIVI ITIYDLLFVPIARAFTG+QSGITTLQRIGTGLVVSA SML
Sbjct: 361 GFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGLQSGITTLQRIGTGLVVSAFSML 420

Query: 421 VATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQVP 480
           VA MVERKRLR+A+EHGLVDRPD+ IPMSFWWLVPQYTLFGLAE FTLVGLQEFFYDQVP
Sbjct: 421 VAAMVERKRLRVADEHGLVDRPDIIIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVP 480

Query: 481 TDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYLLLS 540
           TDLKSMGLAFYTSVLGMGSILSSLLVS IDE+TGGS + NSWFSN+LNKAHLDYFY LLS
Sbjct: 481 TDLKSMGLAFYTSVLGMGSILSSLLVSFIDEVTGGSDQ-NSWFSNNLNKAHLDYFYFLLS 529

BLAST of HG10003386 vs. NCBI nr
Match: XP_022954701.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata] >XP_022954702.1 protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata])

HSP 1 Score: 865.5 bits (2235), Expect = 2.4e-247
Identity = 450/549 (81.97%), Postives = 484/549 (88.16%), Query Frame = 0

Query: 5   TPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPLG 64
           +P L+ TVDGAVDF+G PV R SSGGWRSASFMIGVEVAERFAYYGIG+NLITYLT PLG
Sbjct: 10  SPLLNDTVDGAVDFQGHPVLRSSSGGWRSASFMIGVEVAERFAYYGIGANLITYLTGPLG 69

Query: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLSP 124
           QSVATAAE+VNIWSGISML TLLGAF+ADSFFGRYRTILFSSAIYVLGL+ LSFSALL  
Sbjct: 70  QSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLALLSFSALLPT 129

Query: 125 TIS--------------PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAK 184
           T S              PQFQL+ FFVSLYLIG+GQGGHKPCVQAFGADQFDA  P+EAK
Sbjct: 130 TTSLLCQNSNEISSCSTPQFQLMLFFVSLYLIGVGQGGHKPCVQAFGADQFDAQHPQEAK 189

Query: 185 SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRY 244
           SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIP IMM+IAS +FVFG  TYRY
Sbjct: 190 SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVFVFGKNTYRY 249

Query: 245 SIKTYDKTPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCG 304
           S+K YDK+PF+RIGRVFVSA RNWRASST IF +EE++ DLS Q AGQF           
Sbjct: 250 SVKKYDKSPFVRIGRVFVSAARNWRASSTFIFGQEESAIDLSQQKAGQF----------- 309

Query: 305 RFLNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGA 364
           RFLNKACIVP  SN CGM+CS+SEVEEAKAVLRIFPVW+TVLVF IVFAQDSTFFTKQGA
Sbjct: 310 RFLNKACIVPNHSNLCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGA 369

Query: 365 TMDRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGL 424
           TMDRSI SGFIIPAAA+DSFVPLSIVI ITIYD +FV IARAFTG+QSGITTLQRIGTGL
Sbjct: 370 TMDRSILSGFIIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGVQSGITTLQRIGTGL 429

Query: 425 VVSAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQ 484
           V+SAISMLVA+MVERKRLR+A E GLVDRPD+TIPMSFWWLVPQYTL+GLAE FTLVGLQ
Sbjct: 430 VISAISMLVASMVERKRLRIAEEQGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQ 489

Query: 485 EFFYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHL 540
           EFFYDQ P+DLKSMGLAFYTSVLGMGSILSSLLVSVIDE TG +G+ NSWFS++LNKAHL
Sbjct: 490 EFFYDQCPSDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGANGQ-NSWFSDNLNKAHL 546

BLAST of HG10003386 vs. NCBI nr
Match: XP_022994214.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima])

HSP 1 Score: 863.6 bits (2230), Expect = 9.0e-247
Identity = 449/549 (81.79%), Postives = 484/549 (88.16%), Query Frame = 0

Query: 5   TPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPLG 64
           +P L+ TVDG VDF+G PV R S+GGWRSASFMIGVEVAERFAYYGIG+NLITYLT PLG
Sbjct: 10  SPLLNDTVDGTVDFQGHPVLRSSAGGWRSASFMIGVEVAERFAYYGIGANLITYLTGPLG 69

Query: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLSP 124
           QSVATAAE+VNIWSGISML TLLGAF+ADSFFGRYRTILFSSAIYVLGL+ LSFSALL  
Sbjct: 70  QSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLALLSFSALLPT 129

Query: 125 TIS--------------PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAK 184
           T S              PQFQL+ FFVSLYLIG+GQGGHKPCVQAFGADQFDA  P+EAK
Sbjct: 130 TTSLLCQNSNEISSCSTPQFQLVLFFVSLYLIGVGQGGHKPCVQAFGADQFDAQHPQEAK 189

Query: 185 SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRY 244
           SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIP IMM+IAS +FVFG  TYRY
Sbjct: 190 SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVFVFGKYTYRY 249

Query: 245 SIKTYDKTPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCG 304
           S+K YDK+PF+RIGRVFVSA RNWRASST IFD+EE++ DLS Q AGQF           
Sbjct: 250 SVKKYDKSPFVRIGRVFVSAARNWRASSTFIFDQEESATDLSQQKAGQF----------- 309

Query: 305 RFLNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGA 364
           RFLNKACIVP  SN+CGM+CS+SEVEEAKAVLRIFPVW+TVLVF IVFAQDSTFFTKQGA
Sbjct: 310 RFLNKACIVPNHSNQCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGA 369

Query: 365 TMDRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGL 424
           TMDRSI SGFIIPAAA+DSFVPLSIVI ITIYD +FV IARAFTGIQSGITTLQRIGTGL
Sbjct: 370 TMDRSILSGFIIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGIQSGITTLQRIGTGL 429

Query: 425 VVSAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQ 484
           V+SAISMLVA+MVERKRLR+A E GLVDRPD+TIPMSFWWLVPQYTL+GLAE FTLVGLQ
Sbjct: 430 VISAISMLVASMVERKRLRIAEETGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQ 489

Query: 485 EFFYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHL 540
           EFFYDQ P+DLKSMGLAFYTSVLG GSILSSLLVSVIDE TG +G+ NSWFS++LNKAHL
Sbjct: 490 EFFYDQCPSDLKSMGLAFYTSVLGTGSILSSLLVSVIDEATGANGQ-NSWFSDNLNKAHL 546

BLAST of HG10003386 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.4e-170
Identity = 305/546 (55.86%), Postives = 399/546 (73.08%), Query Frame = 0

Query: 2   ERRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTS 61
           E  TP L+ T    VD++ +P  + SSGGWRSA F+IGVEVAERFAYYGI SNLITYLT 
Sbjct: 9   EAGTPLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTG 68

Query: 62  PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSAL 121
           PLGQS A AA  VN WSG + LL LLGAF+ADSF GR+RTIL +SA+Y++GL  L+ SA+
Sbjct: 69  PLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAM 128

Query: 122 ---------LSPTISPQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSK 181
                    L  + SP+FQ++ FF +LYL+ + QGGHKPCVQAFGADQFD  +P E K+K
Sbjct: 129 IPSDCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAK 188

Query: 182 SSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSI 241
           SSFFNWW+FG+C GT   + V+ Y ++NLSW+LGFGIPCI M++A  + + GT TYR+SI
Sbjct: 189 SSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSI 248

Query: 242 KTYDKTPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRF 301
           +  D++PF+RIG V+V+A++NW  S+  +   EE    +S  ++ QF+           F
Sbjct: 249 RREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFS-----------F 308

Query: 302 LNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATM 361
           LNKA +    S      CS+ E+EEAK+VLR+ P+W+T LV+A+VFAQ  TFFTKQGATM
Sbjct: 309 LNKALVAKNGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATM 368

Query: 362 DRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVV 421
           +RSI  G+ I  A L SF+ LSIVI I IYD + +PIAR+FT    GIT LQRIGTG+ +
Sbjct: 369 ERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFL 428

Query: 422 SAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEF 481
           S ++M+VA +VE KRL+ A ++GLVD PD T+PMS WWLVPQY LFG+ + F +VGLQEF
Sbjct: 429 SFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEF 488

Query: 482 FYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDY 539
           FYDQVP +L+S+GLA Y S+ G+G+ LSS ++S+I++ T  SG+  SWF+N+LN+AHLDY
Sbjct: 489 FYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQ-ASWFANNLNQAHLDY 532

BLAST of HG10003386 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 562.8 bits (1449), Expect = 4.3e-159
Identity = 295/535 (55.14%), Postives = 378/535 (70.65%), Query Frame = 0

Query: 12  VDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPLGQSVATAA 71
           V  AVD +G    R ++G WR+A F+IGVEVAERFAYYGIGSNLI+YLT PLG+S A AA
Sbjct: 14  VTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAA 73

Query: 72  ETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLSP------T 131
             VN WSGI+ LL +LGAF+AD+F GRYRTI+ SS IYVLGL+FL+ SA L P      +
Sbjct: 74  ANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTS 133

Query: 132 ISPQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFNWWFFGVCA 191
            +  F  + FF SLYL+ IGQ GHKPCVQAFGADQFD  D +E   +SSFFNWW+  + A
Sbjct: 134 STSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSA 193

Query: 192 GTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDK--TPFLRI 251
           G   AILVV Y +E  SW+ GFGIPC+ M+I+  LFV G + YRYS + +++   PF RI
Sbjct: 194 GICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRI 253

Query: 252 GRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNKACIVPKDS 311
           GRVF  A++N R SS+ +   E  +     + +               F NKA +VP DS
Sbjct: 254 GRVFFVALKNQRLSSSDLCKVELEANTSPEKQS---------------FFNKALLVPNDS 313

Query: 312 NKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRSIFSGFIIP 371
           ++       S+VE+A A++R+ PVW T L +AI +AQ  TFFTKQG TMDR+I  G  IP
Sbjct: 314 SQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIP 373

Query: 372 AAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISMLVATMV 431
            A+L  F+ +SIV+ + IYD +FVPIAR  T    GITTL+RIGTG+V+S I+M++A +V
Sbjct: 374 PASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALV 433

Query: 432 ERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQVPTDLKS 491
           E KRL  A EHGL+D+P+ T+PMS WWL+PQY L GLA+ +TLVG+QEFFY QVPT+L+S
Sbjct: 434 EFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRS 493

Query: 492 MGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYLLLS 539
           +GLA Y S LG+GS+LSSLL+S+ID  TGG    NSWF+++LN+AHLDYFY LL+
Sbjct: 494 IGLALYLSALGVGSLLSSLLISLIDLATGGDA-GNSWFNSNLNRAHLDYFYWLLA 532

BLAST of HG10003386 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 558.9 bits (1439), Expect = 6.2e-158
Identity = 296/543 (54.51%), Postives = 383/543 (70.53%), Query Frame = 0

Query: 4   RTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPL 63
           +T    + V  AVD +G    R  +G WR+A F+IGVEVAERFA YGIGSNLI+YLT PL
Sbjct: 6   KTSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPL 65

Query: 64  GQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLS 123
           GQS A AA  VN WSGIS +L LLGAF+AD+F GRY TI+ +S IYVLGL+FL+ SA L 
Sbjct: 66  GQSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLI 125

Query: 124 P-----TISP-QFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFN 183
           P     T SP  F    FF SLYL+ IGQ GHKPCVQAFGADQFD  +P+E   +SSFFN
Sbjct: 126 PNNTEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFN 185

Query: 184 WWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDK 243
           WW+  +CAG  +AILVV Y +EN+SW+LGFGIPC+ M+I+  LFV G K+YR+S    ++
Sbjct: 186 WWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEE 245

Query: 244 --TPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNK 303
              PF RIGRVF  A +N R +S+ +   E    + S ++  + +           FLNK
Sbjct: 246 ETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELS-----------FLNK 305

Query: 304 ACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRS 363
           A +VP DS++  + C   +VE+A A++R+ PVW+T L +AI FAQ  TFFTKQG TM+R+
Sbjct: 306 ALLVPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERT 365

Query: 364 IFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAI 423
           IF G  IP A+L   + +SIV+ + IYD + VPI R+ T    GITTL+RIGTG+V++ +
Sbjct: 366 IFPGVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATL 425

Query: 424 SMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYD 483
           +M+VA +VE KRL  A E+GL+D+P  T+PMS WWL PQY L GLA+  TLVG+QEFFY 
Sbjct: 426 TMVVAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYS 485

Query: 484 QVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYL 539
           QVPT+L+S+GLA Y S +G+GS+LSSLL+ +ID  TGG    NSWF+++LN+AHLDYFY 
Sbjct: 486 QVPTELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDA-GNSWFNSNLNRAHLDYFYW 536

BLAST of HG10003386 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.2e-148
Identity = 283/556 (50.90%), Postives = 376/556 (67.63%), Query Frame = 0

Query: 2   ERRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTS 61
           E     +  +V  +VD +G P  + S+GGWRSA ++IGVEV ERFAY+GIGSNLITYLT 
Sbjct: 5   EEEAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTG 64

Query: 62  PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSAL 121
           PLGQS ATAA  VN WSG + +L +LGAF+AD++ GRYRTI+ +S IY+LGL  L+ S++
Sbjct: 65  PLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSI 124

Query: 122 L-------------SPTISPQFQL-LFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPR 181
           L             + +  P F + + FF SLYL+ IGQGGHKPCVQAFGADQFD  DP+
Sbjct: 125 LILMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPK 184

Query: 182 EAKSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKT 241
           E  S+ SFFNWWF  + AG  ++I+VV Y ++N++W+LGFGIPC+ M++A  LF+FG KT
Sbjct: 185 ERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKT 244

Query: 242 YRY--SIKTYDKTPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSW 301
           YRY    +      F RIGRVF+ A +N               R L L ++GQ  V +  
Sbjct: 245 YRYPRGDREGKNNAFARIGRVFLVAFKN---------------RKLKLTHSGQLEVGS-- 304

Query: 302 ILKCG---RFLNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDS 361
             KC     FL KA ++P +       CS  +VE+A A++R+ P+WIT +V  I +AQ +
Sbjct: 305 YKKCKGQLEFLAKA-LLPGEGGV--EPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYA 364

Query: 362 TFFTKQGATMDRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITT 421
           TFFTKQG T+DR I  GF IP A+  + + LSI I +  Y+ +F+P+AR  T   SGIT 
Sbjct: 365 TFFTKQGVTVDRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITM 424

Query: 422 LQRIGTGLVVSAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAE 481
           LQRIG G+V+S+++M+VA +VE KRL  A EHGLVDRPD TIPMS WW VPQY L G+ +
Sbjct: 425 LQRIGAGMVLSSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMID 484

Query: 482 AFTLVGLQEFFYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFS 539
            F+LVG QEFFYDQVPT+L+S+GLA   S +G+ S LS  L++VI+  TG +G  +SWF+
Sbjct: 485 VFSLVGTQEFFYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNG-GDSWFN 539

BLAST of HG10003386 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 4.2e-146
Identity = 279/548 (50.91%), Postives = 368/548 (67.15%), Query Frame = 0

Query: 9   SQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPLGQSVA 68
           ++ V+ +VDF+G P  R SSG W+S+ F +  EVAE+FAY+GI SNLITY T  LG+S A
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 69  TAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLSPTIS- 128
            AA  VN+W G +  L L+   +ADSF GR+RTIL +S+ Y++GL  L+FSA +    + 
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSLCND 135

Query: 129 --------PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFNWW 188
                    Q +++ FF +LYLI +G+GG K C++AFGADQFD  DP E+K+KSS+FNW 
Sbjct: 136 QETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSYFNWL 195

Query: 189 FFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSI------- 248
           +F +  G     LV  Y +ENLSW+LG+ IPC+ M++A FLF+ G KTYR+S        
Sbjct: 196 YFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGEGRQG 255

Query: 249 KTYDKTPFLRIGRVFVSAIRNWR--ASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCG 308
           K +D  PF+RIGRVFV+A RN R   S T +    E+++                     
Sbjct: 256 KKHD-NPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTKKF------------------- 315

Query: 309 RFLNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGA 368
           RFL++A I           C   EVEEAKAVL + P+W+  LVF IVFAQ  TFFTKQG+
Sbjct: 316 RFLDRAVI----------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGS 375

Query: 369 TMDRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGL 428
           TMDRSI S   +PAA L  F+ L+I++ I IYD LFVPIAR+ T   +GITTLQRI TG+
Sbjct: 376 TMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGI 435

Query: 429 VVSAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQ 488
            +S ISM++A +VE KRL+ A +HGLVD P  T+PMS  WL+PQY LFG+++ FT+VGLQ
Sbjct: 436 FLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQ 495

Query: 489 EFFYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHL 539
           EFFY +VP  L+SMGLA Y S++G+G+ LSS +VSVI+E T  SG+  SWFSN+LN+AHL
Sbjct: 496 EFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQ-VSWFSNNLNQAHL 532

BLAST of HG10003386 vs. ExPASy TrEMBL
Match: A0A1S3CAV1 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103498725 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.4e-261
Identity = 477/540 (88.33%), Postives = 496/540 (91.85%), Query Frame = 0

Query: 1   MERRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60
           ME+ TP LSQTV+ AVDFKGQP  R  SGGWRSASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 1   MEQPTPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60

Query: 61  SPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSA 120
            PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLS LSFSA
Sbjct: 61  GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 120

Query: 121 LLSPTIS--PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFNW 180
           +L  T S   QFQL+FFFVSLYLIGIGQGGHKPCVQAFGADQFDAL P+EAKSKSSFFNW
Sbjct: 121 MLPTTSSQNSQFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 180

Query: 181 WFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDKT 240
           WFFGVCAGTFVAIL+VTYTEENLSWSLGFGIPCIMM+IASFLF+FGT TYRYSIK Y KT
Sbjct: 181 WFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGTNTYRYSIKMYAKT 240

Query: 241 PFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNKACI 300
           PFLRIGRVFVSAIRN RASSTV FDEE +  DLS QN GQF+           FLNKACI
Sbjct: 241 PFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFS-----------FLNKACI 300

Query: 301 VPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRSIFS 360
           VPKDSNK G+MCS SEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGAT+DRSI S
Sbjct: 301 VPKDSNKHGVMCSASEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATIDRSILS 360

Query: 361 GFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML 420
           GFIIPAAALDSFVPLSIVI ITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML
Sbjct: 361 GFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML 420

Query: 421 VATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQVP 480
           VATMVERKRLR+A+EHGLVDRPD+TIPMSFWWLVPQYTLFGLAE FTLVGLQEFFYDQVP
Sbjct: 421 VATMVERKRLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVP 480

Query: 481 TDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYLLLS 539
           TDLKSMGLAFYTSVLGMGSILSSLLVS+IDE+TGGS + NSWFSN+LNKAHLDYFYLLLS
Sbjct: 481 TDLKSMGLAFYTSVLGMGSILSSLLVSIIDEVTGGS-EQNSWFSNNLNKAHLDYFYLLLS 528

BLAST of HG10003386 vs. ExPASy TrEMBL
Match: A0A6J1GRM0 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111456880 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.1e-247
Identity = 450/549 (81.97%), Postives = 484/549 (88.16%), Query Frame = 0

Query: 5   TPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPLG 64
           +P L+ TVDGAVDF+G PV R SSGGWRSASFMIGVEVAERFAYYGIG+NLITYLT PLG
Sbjct: 10  SPLLNDTVDGAVDFQGHPVLRSSSGGWRSASFMIGVEVAERFAYYGIGANLITYLTGPLG 69

Query: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLSP 124
           QSVATAAE+VNIWSGISML TLLGAF+ADSFFGRYRTILFSSAIYVLGL+ LSFSALL  
Sbjct: 70  QSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLALLSFSALLPT 129

Query: 125 TIS--------------PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAK 184
           T S              PQFQL+ FFVSLYLIG+GQGGHKPCVQAFGADQFDA  P+EAK
Sbjct: 130 TTSLLCQNSNEISSCSTPQFQLMLFFVSLYLIGVGQGGHKPCVQAFGADQFDAQHPQEAK 189

Query: 185 SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRY 244
           SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIP IMM+IAS +FVFG  TYRY
Sbjct: 190 SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVFVFGKNTYRY 249

Query: 245 SIKTYDKTPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCG 304
           S+K YDK+PF+RIGRVFVSA RNWRASST IF +EE++ DLS Q AGQF           
Sbjct: 250 SVKKYDKSPFVRIGRVFVSAARNWRASSTFIFGQEESAIDLSQQKAGQF----------- 309

Query: 305 RFLNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGA 364
           RFLNKACIVP  SN CGM+CS+SEVEEAKAVLRIFPVW+TVLVF IVFAQDSTFFTKQGA
Sbjct: 310 RFLNKACIVPNHSNLCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGA 369

Query: 365 TMDRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGL 424
           TMDRSI SGFIIPAAA+DSFVPLSIVI ITIYD +FV IARAFTG+QSGITTLQRIGTGL
Sbjct: 370 TMDRSILSGFIIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGVQSGITTLQRIGTGL 429

Query: 425 VVSAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQ 484
           V+SAISMLVA+MVERKRLR+A E GLVDRPD+TIPMSFWWLVPQYTL+GLAE FTLVGLQ
Sbjct: 430 VISAISMLVASMVERKRLRIAEEQGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQ 489

Query: 485 EFFYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHL 540
           EFFYDQ P+DLKSMGLAFYTSVLGMGSILSSLLVSVIDE TG +G+ NSWFS++LNKAHL
Sbjct: 490 EFFYDQCPSDLKSMGLAFYTSVLGMGSILSSLLVSVIDEATGANGQ-NSWFSDNLNKAHL 546

BLAST of HG10003386 vs. ExPASy TrEMBL
Match: A0A6J1K2A2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111490020 PE=3 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 4.3e-247
Identity = 449/549 (81.79%), Postives = 484/549 (88.16%), Query Frame = 0

Query: 5   TPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPLG 64
           +P L+ TVDG VDF+G PV R S+GGWRSASFMIGVEVAERFAYYGIG+NLITYLT PLG
Sbjct: 10  SPLLNDTVDGTVDFQGHPVLRSSAGGWRSASFMIGVEVAERFAYYGIGANLITYLTGPLG 69

Query: 65  QSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLSP 124
           QSVATAAE+VNIWSGISML TLLGAF+ADSFFGRYRTILFSSAIYVLGL+ LSFSALL  
Sbjct: 70  QSVATAAESVNIWSGISMLFTLLGAFVADSFFGRYRTILFSSAIYVLGLALLSFSALLPT 129

Query: 125 TIS--------------PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAK 184
           T S              PQFQL+ FFVSLYLIG+GQGGHKPCVQAFGADQFDA  P+EAK
Sbjct: 130 TTSLLCQNSNEISSCSTPQFQLVLFFVSLYLIGVGQGGHKPCVQAFGADQFDAQHPQEAK 189

Query: 185 SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRY 244
           SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIP IMM+IAS +FVFG  TYRY
Sbjct: 190 SKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPFIMMVIASVVFVFGKYTYRY 249

Query: 245 SIKTYDKTPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCG 304
           S+K YDK+PF+RIGRVFVSA RNWRASST IFD+EE++ DLS Q AGQF           
Sbjct: 250 SVKKYDKSPFVRIGRVFVSAARNWRASSTFIFDQEESATDLSQQKAGQF----------- 309

Query: 305 RFLNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGA 364
           RFLNKACIVP  SN+CGM+CS+SEVEEAKAVLRIFPVW+TVLVF IVFAQDSTFFTKQGA
Sbjct: 310 RFLNKACIVPNHSNQCGMVCSMSEVEEAKAVLRIFPVWMTVLVFGIVFAQDSTFFTKQGA 369

Query: 365 TMDRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGL 424
           TMDRSI SGFIIPAAA+DSFVPLSIVI ITIYD +FV IARAFTGIQSGITTLQRIGTGL
Sbjct: 370 TMDRSILSGFIIPAAAIDSFVPLSIVIFITIYDRVFVFIARAFTGIQSGITTLQRIGTGL 429

Query: 425 VVSAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQ 484
           V+SAISMLVA+MVERKRLR+A E GLVDRPD+TIPMSFWWLVPQYTL+GLAE FTLVGLQ
Sbjct: 430 VISAISMLVASMVERKRLRIAEETGLVDRPDITIPMSFWWLVPQYTLYGLAEVFTLVGLQ 489

Query: 485 EFFYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHL 540
           EFFYDQ P+DLKSMGLAFYTSVLG GSILSSLLVSVIDE TG +G+ NSWFS++LNKAHL
Sbjct: 490 EFFYDQCPSDLKSMGLAFYTSVLGTGSILSSLLVSVIDEATGANGQ-NSWFSDNLNKAHL 546

BLAST of HG10003386 vs. ExPASy TrEMBL
Match: A0A5A7T8K1 (Proton-dependent oligopeptide transporter family OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00050 PE=3 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 2.2e-243
Identity = 451/540 (83.52%), Postives = 470/540 (87.04%), Query Frame = 0

Query: 1   MERRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 60
           ME+ TP LSQTV+ AVDFKGQP  R  SGGWRSASFMIGVEVAERFAYYGIGSNLITYLT
Sbjct: 102 MEQPTPLLSQTVESAVDFKGQPALRFCSGGWRSASFMIGVEVAERFAYYGIGSNLITYLT 161

Query: 61  SPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSA 120
            PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLS LSFSA
Sbjct: 162 GPLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSLLSFSA 221

Query: 121 LLSPTIS--PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFNW 180
           +L  T S   QFQL+FFFVSLYLIGIGQGGHKPCVQAFGADQFDAL P+EAKSKSSFFNW
Sbjct: 222 MLPTTSSQNSQFQLIFFFVSLYLIGIGQGGHKPCVQAFGADQFDALHPQEAKSKSSFFNW 281

Query: 181 WFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDKT 240
           WFFGVCAGTFVAIL+VTYTEENLSWSLGFGIPCIMM+IASFLF+FGT TYRYSIK Y KT
Sbjct: 282 WFFGVCAGTFVAILLVTYTEENLSWSLGFGIPCIMMVIASFLFLFGTNTYRYSIKMYAKT 341

Query: 241 PFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNKACI 300
           PFLRIGRVFVSAIRN RASSTV FDEE +  DLS QN GQF+           FLNKACI
Sbjct: 342 PFLRIGRVFVSAIRNCRASSTVTFDEEGDGPDLSEQNTGQFS-----------FLNKACI 401

Query: 301 VPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRSIFS 360
           VPKDSNK G+MCS SE                          DSTFFTKQGAT+DRSI S
Sbjct: 402 VPKDSNKHGVMCSASE--------------------------DSTFFTKQGATIDRSILS 461

Query: 361 GFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML 420
           GFIIPAAALDSFVPLSIVI ITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML
Sbjct: 462 GFIIPAAALDSFVPLSIVIFITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML 521

Query: 421 VATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQVP 480
           VATMVERKRLR+A+EHGLVDRPD+TIPMSFWWLVPQYTLFGLAE FTLVGLQEFFYDQVP
Sbjct: 522 VATMVERKRLRIADEHGLVDRPDITIPMSFWWLVPQYTLFGLAEVFTLVGLQEFFYDQVP 581

Query: 481 TDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYLLLS 539
           TDLKSMGLAFYTSVLGMGSILSSLLVS+IDE+TGGS + NSWFSN+LNKAHLDYFYLLLS
Sbjct: 582 TDLKSMGLAFYTSVLGMGSILSSLLVSIIDEVTGGS-EQNSWFSNNLNKAHLDYFYLLLS 603

BLAST of HG10003386 vs. ExPASy TrEMBL
Match: A0A6J1DNR2 (protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC111022354 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 3.0e-240
Identity = 429/541 (79.30%), Postives = 475/541 (87.80%), Query Frame = 0

Query: 3   RRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSP 62
           RRTP LS TVDGAVDFKGQPV R SSGGWRSASF+IGVEVAERFAYYGIGSNLITYLT P
Sbjct: 9   RRTPLLSDTVDGAVDFKGQPVLRSSSGGWRSASFIIGVEVAERFAYYGIGSNLITYLTGP 68

Query: 63  LGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALL 122
           LG SVATAAE++N+WSG SMLLTLLGAF+ADSFFG YRTIL SSAIYVLGL  LSFSALL
Sbjct: 69  LGLSVATAAESLNVWSGTSMLLTLLGAFVADSFFGPYRTILLSSAIYVLGLGLLSFSALL 128

Query: 123 SPTISP----QFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFNW 182
             T S     + Q++ FFVSLYLIGIGQGGHKPCVQAFGADQFD   P E KSKSSFFNW
Sbjct: 129 PTTNSSVSHNKLQIILFFVSLYLIGIGQGGHKPCVQAFGADQFDGRHPEEVKSKSSFFNW 188

Query: 183 WFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDKT 242
           WFFG+CAGTFVA+LVVTYTEEN+SWSLGFGIPC+ M+IA  +F+FGT TYRYS+K Y K+
Sbjct: 189 WFFGLCAGTFVAMLVVTYTEENVSWSLGFGIPCLAMVIALVVFLFGTNTYRYSVKEYGKS 248

Query: 243 PFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNKACI 302
           PF+RIGRVFVSAIRNWRAS TV FDEE+  R LS QNAGQF           RFLNKAC 
Sbjct: 249 PFVRIGRVFVSAIRNWRASPTVFFDEEDGGRVLSHQNAGQF-----------RFLNKACA 308

Query: 303 VPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRSIFS 362
           VP  S++CGM CS+SEVEEAKAVLRI P+WIT+LVFAIVFAQD+TFFTKQGATM+RSIFS
Sbjct: 309 VPNHSSQCGMACSISEVEEAKAVLRILPIWITILVFAIVFAQDTTFFTKQGATMNRSIFS 368

Query: 363 GFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISML 422
           GFIIPAAAL+S VPLSIVI I++YDL+FVP+ARAFTGIQSGIT LQRIGTGLV+SAISM+
Sbjct: 369 GFIIPAAALESLVPLSIVIFISVYDLVFVPVARAFTGIQSGITPLQRIGTGLVISAISMV 428

Query: 423 VATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQVP 482
           VATMVERKRLR A EHG+VD PD+TIPMSFWWLVPQY+L+GLA+ FT+VGLQEFFY+Q P
Sbjct: 429 VATMVERKRLRSAEEHGVVDSPDITIPMSFWWLVPQYSLYGLADVFTVVGLQEFFYNQCP 488

Query: 483 TDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYLLLS 540
            DLKSMGLA YTSVLGMGSILSS+LVSVIDE +GG+G +NSWFS++LNKAHLDYFYLLLS
Sbjct: 489 ADLKSMGLALYTSVLGMGSILSSVLVSVIDEASGGNG-HNSWFSDNLNKAHLDYFYLLLS 537

BLAST of HG10003386 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 600.9 bits (1548), Expect = 1.0e-171
Identity = 305/546 (55.86%), Postives = 399/546 (73.08%), Query Frame = 0

Query: 2   ERRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTS 61
           E  TP L+ T    VD++ +P  + SSGGWRSA F+IGVEVAERFAYYGI SNLITYLT 
Sbjct: 9   EAGTPLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTG 68

Query: 62  PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSAL 121
           PLGQS A AA  VN WSG + LL LLGAF+ADSF GR+RTIL +SA+Y++GL  L+ SA+
Sbjct: 69  PLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAM 128

Query: 122 ---------LSPTISPQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSK 181
                    L  + SP+FQ++ FF +LYL+ + QGGHKPCVQAFGADQFD  +P E K+K
Sbjct: 129 IPSDCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAK 188

Query: 182 SSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSI 241
           SSFFNWW+FG+C GT   + V+ Y ++NLSW+LGFGIPCI M++A  + + GT TYR+SI
Sbjct: 189 SSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSI 248

Query: 242 KTYDKTPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRF 301
           +  D++PF+RIG V+V+A++NW  S+  +   EE    +S  ++ QF+           F
Sbjct: 249 RREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFS-----------F 308

Query: 302 LNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATM 361
           LNKA +    S      CS+ E+EEAK+VLR+ P+W+T LV+A+VFAQ  TFFTKQGATM
Sbjct: 309 LNKALVAKNGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATM 368

Query: 362 DRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVV 421
           +RSI  G+ I  A L SF+ LSIVI I IYD + +PIAR+FT    GIT LQRIGTG+ +
Sbjct: 369 ERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFL 428

Query: 422 SAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEF 481
           S ++M+VA +VE KRL+ A ++GLVD PD T+PMS WWLVPQY LFG+ + F +VGLQEF
Sbjct: 429 SFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEF 488

Query: 482 FYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDY 539
           FYDQVP +L+S+GLA Y S+ G+G+ LSS ++S+I++ T  SG+  SWF+N+LN+AHLDY
Sbjct: 489 FYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQ-ASWFANNLNQAHLDY 532

BLAST of HG10003386 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 562.8 bits (1449), Expect = 3.0e-160
Identity = 295/535 (55.14%), Postives = 378/535 (70.65%), Query Frame = 0

Query: 12  VDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPLGQSVATAA 71
           V  AVD +G    R ++G WR+A F+IGVEVAERFAYYGIGSNLI+YLT PLG+S A AA
Sbjct: 14  VTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAA 73

Query: 72  ETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLSP------T 131
             VN WSGI+ LL +LGAF+AD+F GRYRTI+ SS IYVLGL+FL+ SA L P      +
Sbjct: 74  ANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTS 133

Query: 132 ISPQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFNWWFFGVCA 191
            +  F  + FF SLYL+ IGQ GHKPCVQAFGADQFD  D +E   +SSFFNWW+  + A
Sbjct: 134 STSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSA 193

Query: 192 GTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDK--TPFLRI 251
           G   AILVV Y +E  SW+ GFGIPC+ M+I+  LFV G + YRYS + +++   PF RI
Sbjct: 194 GICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRI 253

Query: 252 GRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNKACIVPKDS 311
           GRVF  A++N R SS+ +   E  +     + +               F NKA +VP DS
Sbjct: 254 GRVFFVALKNQRLSSSDLCKVELEANTSPEKQS---------------FFNKALLVPNDS 313

Query: 312 NKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRSIFSGFIIP 371
           ++       S+VE+A A++R+ PVW T L +AI +AQ  TFFTKQG TMDR+I  G  IP
Sbjct: 314 SQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIP 373

Query: 372 AAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAISMLVATMV 431
            A+L  F+ +SIV+ + IYD +FVPIAR  T    GITTL+RIGTG+V+S I+M++A +V
Sbjct: 374 PASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALV 433

Query: 432 ERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYDQVPTDLKS 491
           E KRL  A EHGL+D+P+ T+PMS WWL+PQY L GLA+ +TLVG+QEFFY QVPT+L+S
Sbjct: 434 EFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRS 493

Query: 492 MGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYLLLS 539
           +GLA Y S LG+GS+LSSLL+S+ID  TGG    NSWF+++LN+AHLDYFY LL+
Sbjct: 494 IGLALYLSALGVGSLLSSLLISLIDLATGGDA-GNSWFNSNLNRAHLDYFYWLLA 532

BLAST of HG10003386 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 558.9 bits (1439), Expect = 4.4e-159
Identity = 296/543 (54.51%), Postives = 383/543 (70.53%), Query Frame = 0

Query: 4   RTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPL 63
           +T    + V  AVD +G    R  +G WR+A F+IGVEVAERFA YGIGSNLI+YLT PL
Sbjct: 6   KTSLQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPL 65

Query: 64  GQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLS 123
           GQS A AA  VN WSGIS +L LLGAF+AD+F GRY TI+ +S IYVLGL+FL+ SA L 
Sbjct: 66  GQSTAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLI 125

Query: 124 P-----TISP-QFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFN 183
           P     T SP  F    FF SLYL+ IGQ GHKPCVQAFGADQFD  +P+E   +SSFFN
Sbjct: 126 PNNTEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFN 185

Query: 184 WWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSIKTYDK 243
           WW+  +CAG  +AILVV Y +EN+SW+LGFGIPC+ M+I+  LFV G K+YR+S    ++
Sbjct: 186 WWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEE 245

Query: 244 --TPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCGRFLNK 303
              PF RIGRVF  A +N R +S+ +   E    + S ++  + +           FLNK
Sbjct: 246 ETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELS-----------FLNK 305

Query: 304 ACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGATMDRS 363
           A +VP DS++  + C   +VE+A A++R+ PVW+T L +AI FAQ  TFFTKQG TM+R+
Sbjct: 306 ALLVPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERT 365

Query: 364 IFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGLVVSAI 423
           IF G  IP A+L   + +SIV+ + IYD + VPI R+ T    GITTL+RIGTG+V++ +
Sbjct: 366 IFPGVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATL 425

Query: 424 SMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQEFFYD 483
           +M+VA +VE KRL  A E+GL+D+P  T+PMS WWL PQY L GLA+  TLVG+QEFFY 
Sbjct: 426 TMVVAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYS 485

Query: 484 QVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHLDYFYL 539
           QVPT+L+S+GLA Y S +G+GS+LSSLL+ +ID  TGG    NSWF+++LN+AHLDYFY 
Sbjct: 486 QVPTELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDA-GNSWFNSNLNRAHLDYFYW 536

BLAST of HG10003386 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 528.1 bits (1359), Expect = 8.3e-150
Identity = 283/556 (50.90%), Postives = 376/556 (67.63%), Query Frame = 0

Query: 2   ERRTPFLSQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTS 61
           E     +  +V  +VD +G P  + S+GGWRSA ++IGVEV ERFAY+GIGSNLITYLT 
Sbjct: 5   EEEAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTG 64

Query: 62  PLGQSVATAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSAL 121
           PLGQS ATAA  VN WSG + +L +LGAF+AD++ GRYRTI+ +S IY+LGL  L+ S++
Sbjct: 65  PLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSI 124

Query: 122 L-------------SPTISPQFQL-LFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPR 181
           L             + +  P F + + FF SLYL+ IGQGGHKPCVQAFGADQFD  DP+
Sbjct: 125 LILMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPK 184

Query: 182 EAKSKSSFFNWWFFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKT 241
           E  S+ SFFNWWF  + AG  ++I+VV Y ++N++W+LGFGIPC+ M++A  LF+FG KT
Sbjct: 185 ERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKT 244

Query: 242 YRY--SIKTYDKTPFLRIGRVFVSAIRNWRASSTVIFDEEENSRDLSLQNAGQFNVNNSW 301
           YRY    +      F RIGRVF+ A +N               R L L ++GQ  V +  
Sbjct: 245 YRYPRGDREGKNNAFARIGRVFLVAFKN---------------RKLKLTHSGQLEVGS-- 304

Query: 302 ILKCG---RFLNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDS 361
             KC     FL KA ++P +       CS  +VE+A A++R+ P+WIT +V  I +AQ +
Sbjct: 305 YKKCKGQLEFLAKA-LLPGEGGV--EPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYA 364

Query: 362 TFFTKQGATMDRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITT 421
           TFFTKQG T+DR I  GF IP A+  + + LSI I +  Y+ +F+P+AR  T   SGIT 
Sbjct: 365 TFFTKQGVTVDRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITM 424

Query: 422 LQRIGTGLVVSAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAE 481
           LQRIG G+V+S+++M+VA +VE KRL  A EHGLVDRPD TIPMS WW VPQY L G+ +
Sbjct: 425 LQRIGAGMVLSSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMID 484

Query: 482 AFTLVGLQEFFYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFS 539
            F+LVG QEFFYDQVPT+L+S+GLA   S +G+ S LS  L++VI+  TG +G  +SWF+
Sbjct: 485 VFSLVGTQEFFYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNG-GDSWFN 539

BLAST of HG10003386 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 519.6 bits (1337), Expect = 3.0e-147
Identity = 279/548 (50.91%), Postives = 368/548 (67.15%), Query Frame = 0

Query: 9   SQTVDGAVDFKGQPVFRLSSGGWRSASFMIGVEVAERFAYYGIGSNLITYLTSPLGQSVA 68
           ++ V+ +VDF+G P  R SSG W+S+ F +  EVAE+FAY+GI SNLITY T  LG+S A
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 69  TAAETVNIWSGISMLLTLLGAFLADSFFGRYRTILFSSAIYVLGLSFLSFSALLSPTIS- 128
            AA  VN+W G +  L L+   +ADSF GR+RTIL +S+ Y++GL  L+FSA +    + 
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSLCND 135

Query: 129 --------PQFQLLFFFVSLYLIGIGQGGHKPCVQAFGADQFDALDPREAKSKSSFFNWW 188
                    Q +++ FF +LYLI +G+GG K C++AFGADQFD  DP E+K+KSS+FNW 
Sbjct: 136 QETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSYFNWL 195

Query: 189 FFGVCAGTFVAILVVTYTEENLSWSLGFGIPCIMMLIASFLFVFGTKTYRYSI------- 248
           +F +  G     LV  Y +ENLSW+LG+ IPC+ M++A FLF+ G KTYR+S        
Sbjct: 196 YFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGEGRQG 255

Query: 249 KTYDKTPFLRIGRVFVSAIRNWR--ASSTVIFDEEENSRDLSLQNAGQFNVNNSWILKCG 308
           K +D  PF+RIGRVFV+A RN R   S T +    E+++                     
Sbjct: 256 KKHD-NPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTKKF------------------- 315

Query: 309 RFLNKACIVPKDSNKCGMMCSVSEVEEAKAVLRIFPVWITVLVFAIVFAQDSTFFTKQGA 368
           RFL++A I           C   EVEEAKAVL + P+W+  LVF IVFAQ  TFFTKQG+
Sbjct: 316 RFLDRAVI----------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGS 375

Query: 369 TMDRSIFSGFIIPAAALDSFVPLSIVICITIYDLLFVPIARAFTGIQSGITTLQRIGTGL 428
           TMDRSI S   +PAA L  F+ L+I++ I IYD LFVPIAR+ T   +GITTLQRI TG+
Sbjct: 376 TMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGI 435

Query: 429 VVSAISMLVATMVERKRLRMANEHGLVDRPDLTIPMSFWWLVPQYTLFGLAEAFTLVGLQ 488
            +S ISM++A +VE KRL+ A +HGLVD P  T+PMS  WL+PQY LFG+++ FT+VGLQ
Sbjct: 436 FLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQ 495

Query: 489 EFFYDQVPTDLKSMGLAFYTSVLGMGSILSSLLVSVIDEITGGSGKNNSWFSNDLNKAHL 539
           EFFY +VP  L+SMGLA Y S++G+G+ LSS +VSVI+E T  SG+  SWFSN+LN+AHL
Sbjct: 496 EFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQ-VSWFSNNLNQAHL 532