HG10003321 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003321
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionSte24 endopeptidase
LocationChr11: 20175309 .. 20178281 (-)
RNA-Seq ExpressionHG10003321
SyntenyHG10003321
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCATGGCTTCAATTCCCTTTTCTTCTCTCTGCCTCACCAGAAATCCTATTCCTTGCGGAATCAGATCTCCATCCTCCCACAGATTCAACAATTTCACTACCTTCCGAATCGGATTCGGAATCGAATCGCCGCTCACTAAGAAAATTAGGTCTTCCGTTTGCAGAGCTGCCTCCCTCGCCTTTCGCGACCTTGATGCCGACGATTTTCGTCATCCTCTCGATAAGCAGGTTGGAAGTTTTTCTCGTTTCTTTAACTGACTTCAGAGTGAATTAATCTTTTCAATTCATTATTTCTGCCCTGAAGAATTACGGTGTCATCTGTCTTCCTGGACTTCGACTCAACAATTAATACACATAATGTTCAAGTCTCTAGAAAAACTAAAGGATCGTAAAATTTTCTAATAAAAGAATTCATACACACTTTGAAATTAAACTGAATGACTCCGATGTTAGAAGATAGAAGTAACTTATTTGAAATATCCCTCTTTTTAATAAAAAAAAAATTATGTTGATATCATTCTGAGTCAGATACTTTGTTGCTAAAGTCAAAAATTGTTCTTTCATGCAGAACACAATGATTTTGCGGGCAATTCCTGGGTTGAGCGAGCTGGGGAAGGTTTTATTGGGTAAGACTGAGAACTGAAGAACTTCAGAAATGATTATTGGGCATCCAATTGATTTGGACATACCTATTGGAAACAATTTTCTTGCTTTTGCTGAATGTCCAGTTTGAGTATCCATATTTTATTGTTGGAGCATGACTGAGTTGTTAGTTGTTACAAGAAATCTGATCTGCAACTAGGTTGGAGATGTTCTCATCAAGATGATTAAATCATTAAAGACAGTATATTAACCTTTCAACATAAACATATTATATGTATATGGTCATCTGAGCATAAATTATTTTCTTTTTATCTCCGCAAGCGTGTGAGCTAGCTAGCATGCACCCCAACGATTTTCGAAAGAAAACTTCCTAGTCCTACTACATTTTATTACTAAGAAAGTGCATATGATATTAAATTCTAGGTGGTTGTTGCCCGCAATGTCTTGAATCCATTTTATCTAAGCCCTTTACGATTTACATTGGTCTCATTTTCCACTAGGCCAACCTAAATAGGTTATACGAGCTGAACTTATTTATTTCTTTGATGCAGTATATATTTCAAGTAGTTACTTCCATACTGGTCTGCCGCTCTTTGGCTACATAGTGCTAATATTTTTTTCACCTTTGTTTCGTCATTGCAGGAACTGTGACTGAGCAAGTTATGCTTCTTGAGAACATAGGAACATCAATTCTTGTTTCTGAAAATCAGGTTTTTCAACCCTAAAAATCCATCATCTGACGATAGTACTCTTTCGTAATATGGAGATTGAATGATGAGCATTTGTTGTTCCTTATTATATGTGAACATAATGGGATTGAGCATGGGGATGTTTAGCATATGTTTCTTCCCTTTTCAGCTTTCAGATCTTCATCAAGTGATGATTGAGGCTGCCAAAGTACTGAATGTTGAGGCTCCAGATCTATATGTCCGTCAAAATCCTGTGCCAAATGCTTATACTTTAGCCATAAGTGGTAAAAAGCCGTTTGTTGTTGTTCATACTGGACTTGTGGAGCTTCTTACACGAAAAGAATTGCAGGTCAGCAGTTATCTTATGCTGTTATCTCATTAAGCATCCATGCTAACTGGAATTATGCATCGCTGATGCAGTCTTAGCCTCTTAGGTTGCTGAATCAATTTGTATCTTCAACTTCATACCAAAAAAAGAAAAACAACAAAAATGCCTAGCACATACATCATTAATCAAATACATGCCAAACCATAACGTGGAACTCAAGTATACTGTTATTTATTATTTCATTTTTGCATGTTTGTTGTCCATCCTTTTGTTTCTTATCGATGCTGTCTCATGCTGTAATTTTCTCACTTGAAATATTCAGGCTGTTTTGGCTCATGAATTGGGTCATCTAAAATGTGATCATGGTGTGTGGCTTACATTTGCGAATATTCTTACCCTGGGAGCTTACACTGTACCTGGTAAGCATTTAAATCACGGTCTGATTAGTATAATATAATTATTGGGGCTATTTGTTGATTGGTCCTTTTGGGGAATGACAGAGCACTAACTATCAATTAATGCAATTGTGCATTATTCTAATTCTAGAAGACAGTTATTGAGGGCATTGTTCGTGTGCACACAAAAATTTTAGCATGAAAGTAAATAATTTTTGCTTCATGTCAATCTCGCCACAAACGTTATGATGCGATATTTAAACGTAAAAAAAAAAATCAGGGATTTGTAGGCTCAGGCTAAAAGTATTCAAGTAACGTACCTAAATTTCATTTATTATGTTGTATAGGGCTTGGTGGATTTTTAGCTCAGAACTTAGAAGAACAGTTATTTCGTTGGCTTCGAGCAGCAGAGCTAACTTGTGATCGTGCTGCCCTTCTTGTTGCACAGGATTCTAAAGTACATAGTTTATTTAACTAGAAACTTCATATTCCTTCTGAACGTCTCTCTCTCTCTCTCTCTCTCTCTCCCTCCCTCCCTATCCGTACATGAATTTTATTTTTGAACTTCTTTTCTCATCTATTTGTTTACTCATTTGCTCCAACAAGAGGTGGTCATCTCTGTTCTGATGAAATTAGCTGGGGGCTGTCCATCTATAGCTGATCAACTGAATGTGGATGCCTTTTTGGAGCAAGCTCGCTCTTATGACAAAGCTTCTTCAAGCCCTATAGGATGGTATATAAGGTGAATAATTTGTGAAGCGTATAAAATATCTAGTTATTTCAGTCCATTTCTTATCTAACTGATTGTGTCTGCAGAAATGCTCAAACAAGACAACTCTCACATCCTCTACCTGTTTTACGAGCACGTGAGATTGATGACTGGTCAAAAAGTCAAGAATACAAAAATCTTTTGAAACGGGGAACAAAGATCAGTTATGTTGAAACAGCATAG

mRNA sequence

ATGGCTTCCATGGCTTCAATTCCCTTTTCTTCTCTCTGCCTCACCAGAAATCCTATTCCTTGCGGAATCAGATCTCCATCCTCCCACAGATTCAACAATTTCACTACCTTCCGAATCGGATTCGGAATCGAATCGCCGCTCACTAAGAAAATTAGGTCTTCCGTTTGCAGAGCTGCCTCCCTCGCCTTTCGCGACCTTGATGCCGACGATTTTCGTCATCCTCTCGATAAGCAGAACACAATGATTTTGCGGGCAATTCCTGGGTTGAGCGAGCTGGGGAAGGTTTTATTGGGAACTGTGACTGAGCAAGTTATGCTTCTTGAGAACATAGGAACATCAATTCTTGTTTCTGAAAATCAGCTTTCAGATCTTCATCAAGTGATGATTGAGGCTGCCAAAGTACTGAATGTTGAGGCTCCAGATCTATATGTCCGTCAAAATCCTGTGCCAAATGCTTATACTTTAGCCATAAGTGGTAAAAAGCCGTTTGTTGTTGTTCATACTGGACTTGTGGAGCTTCTTACACGAAAAGAATTGCAGGCTGTTTTGGCTCATGAATTGGGTCATCTAAAATGTGATCATGGTGTGTGGCTTACATTTGCGAATATTCTTACCCTGGGAGCTTACACTGTACCTGGGCTTGGTGGATTTTTAGCTCAGAACTTAGAAGAACAGTTATTTCGTTGGCTTCGAGCAGCAGAGCTAACTTGTGATCGTGCTGCCCTTCTTGTTGCACAGGATTCTAAAGTGGTCATCTCTGTTCTGATGAAATTAGCTGGGGGCTGTCCATCTATAGCTGATCAACTGAATGTGGATGCCTTTTTGGAGCAAGCTCGCTCTTATGACAAAGCTTCTTCAAGCCCTATAGGATGGTATATAAGAAATGCTCAAACAAGACAACTCTCACATCCTCTACCTGTTTTACGAGCACGTGAGATTGATGACTGGTCAAAAAGTCAAGAATACAAAAATCTTTTGAAACGGGGAACAAAGATCAGTTATGTTGAAACAGCATAG

Coding sequence (CDS)

ATGGCTTCCATGGCTTCAATTCCCTTTTCTTCTCTCTGCCTCACCAGAAATCCTATTCCTTGCGGAATCAGATCTCCATCCTCCCACAGATTCAACAATTTCACTACCTTCCGAATCGGATTCGGAATCGAATCGCCGCTCACTAAGAAAATTAGGTCTTCCGTTTGCAGAGCTGCCTCCCTCGCCTTTCGCGACCTTGATGCCGACGATTTTCGTCATCCTCTCGATAAGCAGAACACAATGATTTTGCGGGCAATTCCTGGGTTGAGCGAGCTGGGGAAGGTTTTATTGGGAACTGTGACTGAGCAAGTTATGCTTCTTGAGAACATAGGAACATCAATTCTTGTTTCTGAAAATCAGCTTTCAGATCTTCATCAAGTGATGATTGAGGCTGCCAAAGTACTGAATGTTGAGGCTCCAGATCTATATGTCCGTCAAAATCCTGTGCCAAATGCTTATACTTTAGCCATAAGTGGTAAAAAGCCGTTTGTTGTTGTTCATACTGGACTTGTGGAGCTTCTTACACGAAAAGAATTGCAGGCTGTTTTGGCTCATGAATTGGGTCATCTAAAATGTGATCATGGTGTGTGGCTTACATTTGCGAATATTCTTACCCTGGGAGCTTACACTGTACCTGGGCTTGGTGGATTTTTAGCTCAGAACTTAGAAGAACAGTTATTTCGTTGGCTTCGAGCAGCAGAGCTAACTTGTGATCGTGCTGCCCTTCTTGTTGCACAGGATTCTAAAGTGGTCATCTCTGTTCTGATGAAATTAGCTGGGGGCTGTCCATCTATAGCTGATCAACTGAATGTGGATGCCTTTTTGGAGCAAGCTCGCTCTTATGACAAAGCTTCTTCAAGCCCTATAGGATGGTATATAAGAAATGCTCAAACAAGACAACTCTCACATCCTCTACCTGTTTTACGAGCACGTGAGATTGATGACTGGTCAAAAAGTCAAGAATACAAAAATCTTTTGAAACGGGGAACAAAGATCAGTTATGTTGAAACAGCATAG

Protein sequence

MASMASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAFRDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSDLHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALLVAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSHPLPVLRAREIDDWSKSQEYKNLLKRGTKISYVETA
Homology
BLAST of HG10003321 vs. NCBI nr
Match: XP_038893657.1 (protease HtpX homolog [Benincasa hispida])

HSP 1 Score: 622.9 bits (1605), Expect = 1.7e-174
Identity = 315/335 (94.03%), Postives = 327/335 (97.61%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MASIP+SSLCLT NP+P  I SPSSHRF NFTT RIGF I+SP+T +IRSSVCRAASLAF
Sbjct: 1   MASIPYSSLCLTTNPVPTRIGSPSSHRF-NFTTLRIGFRIQSPVTTRIRSSVCRAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVS+NQLSD
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSKNQLSD 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LHQ+MIEAAKVLNVEAPDLYVRQNPVPNAYTLAI+GKKPF+VVHTGLVELLT+KELQAVL
Sbjct: 121 LHQLMIEAAKVLNVEAPDLYVRQNPVPNAYTLAINGKKPFIVVHTGLVELLTQKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDSKVVISVLMKLAGGCPS+ADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSKVVISVLMKLAGGCPSMADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVETA 339
           PLPVLRAREIDDWSKSQEYKNLLKRGTKI+YVETA
Sbjct: 301 PLPVLRAREIDDWSKSQEYKNLLKRGTKINYVETA 334

BLAST of HG10003321 vs. NCBI nr
Match: XP_004145667.1 (uncharacterized protein LOC101216014 isoform X1 [Cucumis sativus] >KGN58175.1 hypothetical protein Csa_017476 [Cucumis sativus])

HSP 1 Score: 609.4 bits (1570), Expect = 1.9e-170
Identity = 309/335 (92.24%), Postives = 323/335 (96.42%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MAS+P SSLCLT NP+P GI S SS+RF +FTTF I FGI+SP+TK+IRSSVC AASLAF
Sbjct: 1   MASVPLSSLCLTTNPVPRGIGSSSSYRF-SFTTFGIRFGIQSPVTKRIRSSVCAAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTV EQVMLLENIGTSILVSENQLSD
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVAEQVMLLENIGTSILVSENQLSD 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LHQ+MIEAA+VLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLT KELQAVL
Sbjct: 121 LHQLMIEAAEVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTGKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILT+GAYTVPGLGGFLA+NLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTVGAYTVPGLGGFLARNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVETA 339
           PLPVLRARE+DDWSK QEYKNLLKRGTKI++VETA
Sbjct: 301 PLPVLRAREVDDWSKGQEYKNLLKRGTKINFVETA 334

BLAST of HG10003321 vs. NCBI nr
Match: XP_008450100.1 (PREDICTED: protease HtpX isoform X1 [Cucumis melo])

HSP 1 Score: 604.4 bits (1557), Expect = 6.1e-169
Identity = 308/335 (91.94%), Postives = 321/335 (95.82%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MASIPFSSLCLT NP+P GI S SS+RF++ TTF  GFGI+S +TK+IRSSVC AASLAF
Sbjct: 1   MASIPFSSLCLTTNPVPRGIGSSSSYRFSS-TTFGNGFGIQSSVTKRIRSSVCVAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTV EQVMLLENIGTSILVSENQLSD
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVAEQVMLLENIGTSILVSENQLSD 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LH++MIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLT KELQAVL
Sbjct: 121 LHKLMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTEKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILT+GAYTVPG GGFLA+NLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTVGAYTVPGFGGFLARNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVETA 339
           PLPVLRAREIDDWSK QEYKNLLKRGTKI++VE A
Sbjct: 301 PLPVLRAREIDDWSKGQEYKNLLKRGTKINFVEAA 334

BLAST of HG10003321 vs. NCBI nr
Match: KAA0042910.1 (protease HtpX isoform X1 [Cucumis melo var. makuwa] >TYK05312.1 protease HtpX isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 603.6 bits (1555), Expect = 1.0e-168
Identity = 307/335 (91.64%), Postives = 322/335 (96.12%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MASIPFSSLCLT NP+P GI S SS+RF++ TTF  GFGI+S +TK+I+SSVC AASLAF
Sbjct: 1   MASIPFSSLCLTTNPVPRGIGSSSSYRFSS-TTFGNGFGIQSSVTKRIKSSVCVAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTV EQVMLLENIGTSILVSENQLSD
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVAEQVMLLENIGTSILVSENQLSD 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LH++MIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLT KELQAVL
Sbjct: 121 LHKLMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTEKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILT+GAYTVPGLGGFLA+NLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTVGAYTVPGLGGFLARNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYD+ASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDRASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVETA 339
           PLPVLRAREIDDWSK QEYKNLLKRGTKI++VE A
Sbjct: 301 PLPVLRAREIDDWSKGQEYKNLLKRGTKINFVEAA 334

BLAST of HG10003321 vs. NCBI nr
Match: XP_022935367.1 (uncharacterized protein LOC111442272 isoform X2 [Cucurbita moschata])

HSP 1 Score: 593.6 bits (1529), Expect = 1.1e-165
Identity = 305/334 (91.32%), Postives = 318/334 (95.21%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MAS+PFSSL L RN  PC I S  SHRF NFTT    FGI +P+TK+IRSSVCRAASLAF
Sbjct: 1   MASLPFSSLYLARNSTPCTIGSRPSHRF-NFTT----FGIGAPITKRIRSSVCRAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLS+
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSE 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LH++MIEAA+VLNVEAPDLYVRQN  PNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL
Sbjct: 121 LHKLMIEAAQVLNVEAPDLYVRQNSGPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDS+VVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSQVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVET 338
           PLPVLRAREIDDWSK+QEYKN+LKRGTKI++VET
Sbjct: 301 PLPVLRAREIDDWSKTQEYKNILKRGTKINFVET 329

BLAST of HG10003321 vs. ExPASy Swiss-Prot
Match: Q8TP15 (Protease HtpX homolog 2 OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=htpX2 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 4.2e-11
Identity = 56/180 (31.11%), Postives = 83/180 (46.11%), Query Frame = 0

Query: 105 MLLENIGTSILVSENQLSDLHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFV 164
           M+L   G  I VSE++   LH ++     + ++  P + + Q  VPNA+    S  K  V
Sbjct: 61  MVLWTTGAHI-VSESEAPQLHDMVTRLCVIADIPKPQIAIVQTRVPNAFATGRSPNKAVV 120

Query: 165 VVHTGLVELLTRKELQAVLAHELGHLKCDHGVWLTFAN-ILTLGAYTV------PGLGGF 224
            V TG+++ LT  EL+AVLAHEL H+K      LT A+ I T+  Y V       G+GG 
Sbjct: 121 AVTTGIMDKLTPAELEAVLAHELSHVKNRDMAVLTIASFISTIAFYIVRYSLYFGGMGGD 180

Query: 225 LAQNLEEQLFRWL-----------------RAAELTCDRAALLVAQDSKVVISVLMKLAG 261
             ++    L  WL                 R  E   DR + ++      + S LMK++G
Sbjct: 181 RRRDGGGILLVWLVSIAVWVVSFLLIRALSRYREFAADRGSAIITGQPANLASALMKISG 239

BLAST of HG10003321 vs. ExPASy Swiss-Prot
Match: Q8PSE5 (Protease HtpX homolog 2 OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=htpX2 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.3e-09
Identity = 51/180 (28.33%), Postives = 82/180 (45.56%), Query Frame = 0

Query: 105 MLLENIGTSILVSENQLSDLHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFV 164
           M+L   G  I VSE++   LH ++     + ++  P + + +  VPNA+    +  K  V
Sbjct: 61  MVLWTTGAQI-VSESEAPQLHGMITRLCAIADLPKPQVAIVRTQVPNAFATGRNQNKAVV 120

Query: 165 VVHTGLVELLTRKELQAVLAHELGHLKCDHGVWLTFANILTLGAYTV-------PGLGGF 224
            V TGL++ L+  EL+AVLAHEL H+K      LT A+ L+  A+ +         +GG 
Sbjct: 121 AVTTGLMDKLSPAELEAVLAHELSHVKNRDMAVLTIASFLSSVAFYIVRYSLYFGNMGGG 180

Query: 225 LAQNLEEQLFRWL-----------------RAAELTCDRAALLVAQDSKVVISVLMKLAG 261
             +     +  WL                 R  E + DR A ++      + S LMK++G
Sbjct: 181 RRKEGGGIMLVWLVSIVVWIVSFLLIRALSRYREFSADRGAAVITGQPANLASALMKISG 239

BLAST of HG10003321 vs. ExPASy Swiss-Prot
Match: B3QED3 (Protease HtpX homolog OS=Rhodopseudomonas palustris (strain TIE-1) OX=395960 GN=htpX PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 4.3e-08
Identity = 34/108 (31.48%), Postives = 54/108 (50.00%), Query Frame = 0

Query: 116 VSENQLSDLHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLT 175
           V E    DL++++ E A   ++  P +++  NP PNA+    + +   V V TGL+  L+
Sbjct: 61  VDERSAPDLYRMVAELAGRASLPMPRVFIMDNPQPNAFATGRNPENAAVAVTTGLMNQLS 120

Query: 176 RKELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLE 224
           R+EL  V+AHEL H+K    + +T    +      V   G F   N E
Sbjct: 121 REELAGVVAHELAHIKNHDTLLMTITATIAGAISMVAQFGMFFGGNRE 168

BLAST of HG10003321 vs. ExPASy Swiss-Prot
Match: Q3SW84 (Protease HtpX homolog OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=htpX PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 5.7e-08
Identity = 32/99 (32.32%), Postives = 51/99 (51.52%), Query Frame = 0

Query: 123 DLHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAV 182
           DLH+++ E A    +  P ++V  NP PNA+    + +   V V TGL++ L+R+EL  V
Sbjct: 68  DLHRLVAELASRAGLPMPRVFVMDNPQPNAFATGRNPENAAVAVTTGLMQSLSREELAGV 127

Query: 183 LAHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQN 222
           +AHEL H+K    + +T    +      +   G F   N
Sbjct: 128 IAHELAHIKHHDTLLMTITATIAGAISMLAQFGMFFGGN 166

BLAST of HG10003321 vs. ExPASy Swiss-Prot
Match: Q8R936 (Protease HtpX homolog OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=htpX PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.6e-07
Identity = 26/68 (38.24%), Postives = 40/68 (58.82%), Query Frame = 0

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LH ++ E A    V  P +YV   P PNA+      K   + V TGL++++ R+ELQ V+
Sbjct: 85  LHNIVEEVALAAGVPKPKVYVMNEPQPNAFATGKDPKHASICVTTGLLQMMNREELQGVI 144

Query: 184 AHELGHLK 192
           AHE+ H++
Sbjct: 145 AHEMSHIR 152

BLAST of HG10003321 vs. ExPASy TrEMBL
Match: A0A0A0L8A1 (Ste24 endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_3G585870 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 9.3e-171
Identity = 309/335 (92.24%), Postives = 323/335 (96.42%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MAS+P SSLCLT NP+P GI S SS+RF +FTTF I FGI+SP+TK+IRSSVC AASLAF
Sbjct: 1   MASVPLSSLCLTTNPVPRGIGSSSSYRF-SFTTFGIRFGIQSPVTKRIRSSVCAAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTV EQVMLLENIGTSILVSENQLSD
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVAEQVMLLENIGTSILVSENQLSD 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LHQ+MIEAA+VLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLT KELQAVL
Sbjct: 121 LHQLMIEAAEVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTGKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILT+GAYTVPGLGGFLA+NLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTVGAYTVPGLGGFLARNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVETA 339
           PLPVLRARE+DDWSK QEYKNLLKRGTKI++VETA
Sbjct: 301 PLPVLRAREVDDWSKGQEYKNLLKRGTKINFVETA 334

BLAST of HG10003321 vs. ExPASy TrEMBL
Match: A0A1S3BNJ0 (Ste24 endopeptidase OS=Cucumis melo OX=3656 GN=LOC103491787 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.0e-169
Identity = 308/335 (91.94%), Postives = 321/335 (95.82%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MASIPFSSLCLT NP+P GI S SS+RF++ TTF  GFGI+S +TK+IRSSVC AASLAF
Sbjct: 1   MASIPFSSLCLTTNPVPRGIGSSSSYRFSS-TTFGNGFGIQSSVTKRIRSSVCVAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTV EQVMLLENIGTSILVSENQLSD
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVAEQVMLLENIGTSILVSENQLSD 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LH++MIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLT KELQAVL
Sbjct: 121 LHKLMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTEKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILT+GAYTVPG GGFLA+NLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTVGAYTVPGFGGFLARNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVETA 339
           PLPVLRAREIDDWSK QEYKNLLKRGTKI++VE A
Sbjct: 301 PLPVLRAREIDDWSKGQEYKNLLKRGTKINFVEAA 334

BLAST of HG10003321 vs. ExPASy TrEMBL
Match: A0A5D3C2A3 (Ste24 endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold108G001330 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.1e-169
Identity = 307/335 (91.64%), Postives = 322/335 (96.12%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MASIPFSSLCLT NP+P GI S SS+RF++ TTF  GFGI+S +TK+I+SSVC AASLAF
Sbjct: 1   MASIPFSSLCLTTNPVPRGIGSSSSYRFSS-TTFGNGFGIQSSVTKRIKSSVCVAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTV EQVMLLENIGTSILVSENQLSD
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVAEQVMLLENIGTSILVSENQLSD 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LH++MIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLT KELQAVL
Sbjct: 121 LHKLMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTEKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILT+GAYTVPGLGGFLA+NLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTVGAYTVPGLGGFLARNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYD+ASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDRASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVETA 339
           PLPVLRAREIDDWSK QEYKNLLKRGTKI++VE A
Sbjct: 301 PLPVLRAREIDDWSKGQEYKNLLKRGTKINFVEAA 334

BLAST of HG10003321 vs. ExPASy TrEMBL
Match: A0A6J1F5C2 (Ste24 endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111442272 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.3e-166
Identity = 305/334 (91.32%), Postives = 318/334 (95.21%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MAS+PFSSL L RN  PC I S  SHRF NFTT    FGI +P+TK+IRSSVCRAASLAF
Sbjct: 1   MASLPFSSLYLARNSTPCTIGSRPSHRF-NFTT----FGIGAPITKRIRSSVCRAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLS+
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSE 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LH++MIEAA+VLNVEAPDLYVRQN  PNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL
Sbjct: 121 LHKLMIEAAQVLNVEAPDLYVRQNSGPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDS+VVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSQVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVET 338
           PLPVLRAREIDDWSK+QEYKN+LKRGTKI++VET
Sbjct: 301 PLPVLRAREIDDWSKTQEYKNILKRGTKINFVET 329

BLAST of HG10003321 vs. ExPASy TrEMBL
Match: A0A6J1DQS3 (Ste24 endopeptidase OS=Momordica charantia OX=3673 GN=LOC111023380 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.8e-165
Identity = 302/335 (90.15%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 4   MASIPFSSLCLTRNPIPCGIRSPSSHRFNNFTTFRIGFGIESPLTKKIRSSVCRAASLAF 63
           MAS+PFSSLCLTRNP PCGI   + HRF +FT F       SP++K+ R  VCRAASLAF
Sbjct: 1   MASLPFSSLCLTRNPSPCGIGFGAPHRF-DFTAF------GSPVSKRNRYPVCRAASLAF 60

Query: 64  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSD 123
           RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLS+
Sbjct: 61  RDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSILVSENQLSN 120

Query: 124 LHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELLTRKELQAVL 183
           LHQ+MIEAAKVLNV+APDLYVRQ+PVPNAYTLAISGKKPFVVVHTGLVELLT KELQAVL
Sbjct: 121 LHQLMIEAAKVLNVDAPDLYVRQSPVPNAYTLAISGKKPFVVVHTGLVELLTGKELQAVL 180

Query: 184 AHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 243
           AHELGHLKCDHGVWLTFANILTLGAY+VPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL
Sbjct: 181 AHELGHLKCDHGVWLTFANILTLGAYSVPGLGGFLAQNLEEQLFRWLRAAELTCDRAALL 240

Query: 244 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 303
           VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH
Sbjct: 241 VAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIRNAQTRQLSH 300

Query: 304 PLPVLRAREIDDWSKSQEYKNLLKRGTKISYVETA 339
           PLPVLRAREIDDWSKSQEYKN+LKRGTKI++V+TA
Sbjct: 301 PLPVLRAREIDDWSKSQEYKNILKRGTKINFVDTA 328

BLAST of HG10003321 vs. TAIR 10
Match: AT3G27110.1 (Peptidase family M48 family protein )

HSP 1 Score: 484.2 bits (1245), Expect = 8.6e-137
Identity = 247/342 (72.22%), Postives = 283/342 (82.75%), Query Frame = 0

Query: 1   MASMASIPFSSLCLTRN---PIPCGIRSPSSHRFNN--FTTFRIGFGIESPLTKKIRSSV 60
           MA   S P  SLC  ++       G R P    F++   +   IGFG E     ++R  +
Sbjct: 1   MAVSVSAPVLSLCYNQSGELSRSLGYRLPKKVGFSSGRRSVSYIGFGAEKVGRFRVRVPI 60

Query: 61  CRAA-SLAFRDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSI 120
           CRA   L F+DLDADDFRHP DKQNT++LRAIPGL+E GK LLG++TEQ+MLLENIGTS+
Sbjct: 61  CRAVPPLLFKDLDADDFRHPFDKQNTLLLRAIPGLNEFGKALLGSMTEQIMLLENIGTSV 120

Query: 121 LVSENQLSDLHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELL 180
           LVS+NQLSDLH +++EAA++LN+EAPDLYVRQ+PVPNAYTLAISGKKPF+VVHT L+ELL
Sbjct: 121 LVSKNQLSDLHGLLVEAAEILNIEAPDLYVRQSPVPNAYTLAISGKKPFIVVHTSLIELL 180

Query: 181 TRKELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAE 240
           T  ELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVP  G  +A+ LEEQL RWLR+AE
Sbjct: 181 TSAELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVPAFGQMIARTLEEQLLRWLRSAE 240

Query: 241 LTCDRAALLVAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIR 300
           LTCDRAALLVAQD KVV+SVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSP+GWYIR
Sbjct: 241 LTCDRAALLVAQDPKVVVSVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPLGWYIR 300

Query: 301 NAQTRQLSHPLPVLRAREIDDWSKSQEYKNLLKRGTKISYVE 337
           NAQT QLSHPLPVLRAREID+WS+S EYK+LLKR  + S V+
Sbjct: 301 NAQTSQLSHPLPVLRAREIDEWSRSLEYKSLLKRANRKSTVQ 342

BLAST of HG10003321 vs. TAIR 10
Match: AT3G27110.2 (Peptidase family M48 family protein )

HSP 1 Score: 484.2 bits (1245), Expect = 8.6e-137
Identity = 247/342 (72.22%), Postives = 283/342 (82.75%), Query Frame = 0

Query: 1   MASMASIPFSSLCLTRN---PIPCGIRSPSSHRFNN--FTTFRIGFGIESPLTKKIRSSV 60
           MA   S P  SLC  ++       G R P    F++   +   IGFG E     ++R  +
Sbjct: 1   MAVSVSAPVLSLCYNQSGELSRSLGYRLPKKVGFSSGRRSVSYIGFGAEKVGRFRVRVPI 60

Query: 61  CRAA-SLAFRDLDADDFRHPLDKQNTMILRAIPGLSELGKVLLGTVTEQVMLLENIGTSI 120
           CRA   L F+DLDADDFRHP DKQNT++LRAIPGL+E GK LLG++TEQ+MLLENIGTS+
Sbjct: 61  CRAVPPLLFKDLDADDFRHPFDKQNTLLLRAIPGLNEFGKALLGSMTEQIMLLENIGTSV 120

Query: 121 LVSENQLSDLHQVMIEAAKVLNVEAPDLYVRQNPVPNAYTLAISGKKPFVVVHTGLVELL 180
           LVS+NQLSDLH +++EAA++LN+EAPDLYVRQ+PVPNAYTLAISGKKPF+VVHT L+ELL
Sbjct: 121 LVSKNQLSDLHGLLVEAAEILNIEAPDLYVRQSPVPNAYTLAISGKKPFIVVHTSLIELL 180

Query: 181 TRKELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVPGLGGFLAQNLEEQLFRWLRAAE 240
           T  ELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVP  G  +A+ LEEQL RWLR+AE
Sbjct: 181 TSAELQAVLAHELGHLKCDHGVWLTFANILTLGAYTVPAFGQMIARTLEEQLLRWLRSAE 240

Query: 241 LTCDRAALLVAQDSKVVISVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPIGWYIR 300
           LTCDRAALLVAQD KVV+SVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSP+GWYIR
Sbjct: 241 LTCDRAALLVAQDPKVVVSVLMKLAGGCPSIADQLNVDAFLEQARSYDKASSSPLGWYIR 300

Query: 301 NAQTRQLSHPLPVLRAREIDDWSKSQEYKNLLKRGTKISYVE 337
           NAQT QLSHPLPVLRAREID+WS+S EYK+LLKR  + S V+
Sbjct: 301 NAQTSQLSHPLPVLRAREIDEWSRSLEYKSLLKRANRKSTVQ 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893657.11.7e-17494.03protease HtpX homolog [Benincasa hispida][more]
XP_004145667.11.9e-17092.24uncharacterized protein LOC101216014 isoform X1 [Cucumis sativus] >KGN58175.1 hy... [more]
XP_008450100.16.1e-16991.94PREDICTED: protease HtpX isoform X1 [Cucumis melo][more]
KAA0042910.11.0e-16891.64protease HtpX isoform X1 [Cucumis melo var. makuwa] >TYK05312.1 protease HtpX is... [more]
XP_022935367.11.1e-16591.32uncharacterized protein LOC111442272 isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8TP154.2e-1131.11Protease HtpX homolog 2 OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2... [more]
Q8PSE52.3e-0928.33Protease HtpX homolog 2 OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 ... [more]
B3QED34.3e-0831.48Protease HtpX homolog OS=Rhodopseudomonas palustris (strain TIE-1) OX=395960 GN=... [more]
Q3SW845.7e-0832.32Protease HtpX homolog OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237... [more]
Q8R9361.6e-0738.24Protease HtpX homolog OS=Caldanaerobacter subterraneus subsp. tengcongensis (str... [more]
Match NameE-valueIdentityDescription
A0A0A0L8A19.3e-17192.24Ste24 endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_3G585870 PE=3 SV=1[more]
A0A1S3BNJ03.0e-16991.94Ste24 endopeptidase OS=Cucumis melo OX=3656 GN=LOC103491787 PE=3 SV=1[more]
A0A5D3C2A35.1e-16991.64Ste24 endopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold108G... [more]
A0A6J1F5C25.3e-16691.32Ste24 endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111442272 PE=3 SV=1[more]
A0A6J1DQS35.8e-16590.15Ste24 endopeptidase OS=Momordica charantia OX=3673 GN=LOC111023380 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G27110.18.6e-13772.22Peptidase family M48 family protein [more]
AT3G27110.28.6e-13772.22Peptidase family M48 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.2010.10Metalloproteases ("zincins"), catalytic domaincoord: 115..202
e-value: 5.8E-27
score: 95.5
NoneNo IPR availablePANTHERPTHR10120:SF26OS01G0970700 PROTEINcoord: 39..332
NoneNo IPR availableCDDcd07325M48_Ste24p_likecoord: 110..315
e-value: 9.88301E-80
score: 239.819
IPR001915Peptidase M48PFAMPF01435Peptidase_M48coord: 122..316
e-value: 8.7E-21
score: 74.8
IPR027057CAAX prenyl protease 1PANTHERPTHR10120CAAX PRENYL PROTEASE 1coord: 39..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003321.1HG10003321.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071586 CAAX-box protein processing
biological_process GO:1900055 regulation of leaf senescence
biological_process GO:0006508 proteolysis
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008233 peptidase activity