HG10003275 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10003275 vs. NCBI nr
Match: XP_038904154.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 672/745 (90.20%), Postives = 701/745 (94.09%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SYIVYLGSHSHGLNPSAID Q+ATESHYNLLGS LGSNEAAKEAIFYSY+RHINGFAAVL
Sbjct: 29  SYIVYLGSHSHGLNPSAIDAQLATESHYNLLGSLLGSNEAAKEAIFYSYNRHINGFAAVL 88

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           DQKVA+DL KHPDVVSVHENKG+KLHTTLSWNFLGVEN+GAIPSNSIW+LASFGESTII 
Sbjct: 89  DQKVASDLTKHPDVVSVHENKGKKLHTTLSWNFLGVENNGAIPSNSIWNLASFGESTIIA 148

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAGPLNS 189
           NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAI GPLNS
Sbjct: 149 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIVGPLNS 208

Query: 190 SYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLFVG 249
           SYE+ARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKA VAAYKVCWP +   G
Sbjct: 209 SYESARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKALVAAYKVCWPQVFLFG 268

Query: 250 ACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGNSG 309
            CFEADILAGFEAAISDGVDVLSVSLGG   DF  DSI+IGAFHAVQNGITVVCSAGNSG
Sbjct: 269 ECFEADILAGFEAAISDGVDVLSVSLGGDPSDFAQDSISIGAFHAVQNGITVVCSAGNSG 328

Query: 310 PTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISAVD 369
           P PGSVSNVAPWIITVGASTIDRLFTSY+ LGDK+HI G SLSDKILPEQK YPLIS++D
Sbjct: 329 PIPGSVSNVAPWIITVGASTIDRLFTSYVALGDKKHIKGASLSDKILPEQKFYPLISSLD 388

Query: 370 AKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILANDEE 429
           AKANN    +AL+CEE SLDPKKVKGKIV+CLR   ARVDKGFVAAEAGAVGMILAND+E
Sbjct: 389 AKANNVSNYHALICEEGSLDPKKVKGKIVVCLRGINARVDKGFVAAEAGAVGMILANDKE 448

Query: 430 NGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSR 489
           NGDELLADAH+LPASHITYSDGQLVYQYINSTKIPMAY+THVRTELGVKPAPVMASFSSR
Sbjct: 449 NGDELLADAHLLPASHITYSDGQLVYQYINSTKIPMAYLTHVRTELGVKPAPVMASFSSR 508

Query: 490 GPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIV 549
           GPNTIDPSILKPDITAPGVNILAAYSED SP+GSS DKRRVAFNV+SGTSMSCPHISGIV
Sbjct: 509 GPNTIDPSILKPDITAPGVNILAAYSEDASPSGSSFDKRRVAFNVESGTSMSCPHISGIV 568

Query: 550 GLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAANPG 609
           GLLKTLYP WSPAAIRSAIMTTAGTKANDLNPILSTKQ KANA AYGAGHVRPN+AANPG
Sbjct: 569 GLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTKQEKANAFAYGAGHVRPNKAANPG 628

Query: 610 LVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEAVV 669
           LVYDLSTKDYLNYLCA GYN+TQIK FSNDTSFVCSKSFK TD NYPSISIP+LKSEAVV
Sbjct: 629 LVYDLSTKDYLNYLCALGYNKTQIKQFSNDTSFVCSKSFKTTDLNYPSISIPDLKSEAVV 688

Query: 670 NITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFRQK 729
            I RR+KNVGSPGTYVVQVNAPPGVSV VEPTSLKFTGIDEEKSFRVV+KSSVPNDFRQ 
Sbjct: 689 KIKRRLKNVGSPGTYVVQVNAPPGVSVFVEPTSLKFTGIDEEKSFRVVLKSSVPNDFRQM 748

Query: 730 YVFGGLEWSDGIHRVRSPIVVRLGG 755
           YVFG +EWSDG HRV+SPIVVR+GG
Sbjct: 749 YVFGRIEWSDGNHRVKSPIVVRVGG 773

BLAST of HG10003275 vs. NCBI nr
Match: KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 621/745 (83.36%), Postives = 668/745 (89.66%), Query Frame = 0

Query: 10   SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
            SYIVYLGSHSHG NPS+ D QIATESH+NLLGSFLGSNE AKEAIFYSY+RHINGFAAV+
Sbjct: 1459 SYIVYLGSHSHGFNPSSTDAQIATESHFNLLGSFLGSNEEAKEAIFYSYNRHINGFAAVV 1518

Query: 70   DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
            DQKVA DLAKHPDVVSV ENKGRKLHTT SW FLG+EN+GAIPSNS+W+LASFGESTIIG
Sbjct: 1519 DQKVAEDLAKHPDVVSVLENKGRKLHTTNSWKFLGLENNGAIPSNSLWNLASFGESTIIG 1578

Query: 130  NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAGPLNS 189
            NLDTGVWPESKSF+D+ YGPIP+RWKGSCEGGSKF+CNRKLIGARY+NKGYAAI GPLNS
Sbjct: 1579 NLDTGVWPESKSFSDKEYGPIPSRWKGSCEGGSKFYCNRKLIGARYYNKGYAAIVGPLNS 1638

Query: 190  SYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLFVG 249
            SYE+ARDHEGHGTHTLSTAGGHFV  AN+FGYGNGTAKGGSPKA VAAYKVCWP +LF G
Sbjct: 1639 SYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPQVLFFG 1698

Query: 250  ACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGNSG 309
             CF+ADILAGFEAAI DGVDVLSVSLGG   DF  DSI+IG+FHAVQNGI VVCSAGNSG
Sbjct: 1699 ECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFAKDSISIGSFHAVQNGIVVVCSAGNSG 1758

Query: 310  PTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISAVD 369
            PTPGSVSNVAPWIITVGAST DRL+TSY+ +GDKRH  G S+SDK LP QK YPLIS++D
Sbjct: 1759 PTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHFKGASVSDKKLPVQKFYPLISSLD 1818

Query: 370  AKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILANDEE 429
            AKA N  ++ AL+CEE SLDPKKV GKI+ICLR   ARV KG+VAA+AGAVGMILAN EE
Sbjct: 1819 AKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRGDNARVAKGYVAAKAGAVGMILANAEE 1878

Query: 430  NGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSR 489
            NGDE+LADAH+LPASHITYSDGQLVYQYINSTKIPMAYMTHVRTE G+KPAPVMASFSSR
Sbjct: 1879 NGDEILADAHLLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTEEGIKPAPVMASFSSR 1938

Query: 490  GPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIV 549
            GPNT+DPSILKPDITAPG NILAAYS D SPTG+  DKRRV FNV+SGTSMSCPH+SGIV
Sbjct: 1939 GPNTVDPSILKPDITAPGENILAAYSRDASPTGTDFDKRRVPFNVESGTSMSCPHVSGIV 1998

Query: 550  GLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAANPG 609
            GLLKTLYP WSPAAIRSAIMTTAGTKANDL PILST Q KANA AYGAGHV PN AA+PG
Sbjct: 1999 GLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILSTNQEKANAFAYGAGHVSPNRAADPG 2058

Query: 610  LVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEAVV 669
            LVYDLSTKDYLNYLCA+GYN  QIK FSNDTSFVCSKSFK+TD NYPSISIP L+ +  V
Sbjct: 2059 LVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFVCSKSFKLTDLNYPSISIPILEYDVAV 2118

Query: 670  NITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFRQK 729
             I R++KNVGSPGTYVVQV  P GVSVSVEPTSLKFTGIDEEKSFRVV+KS  PN    K
Sbjct: 2119 KIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSYEPNGSGPK 2178

Query: 730  YVFGGLEWSDGIHRVRSPIVVRLGG 755
            Y+FG LEWSDG HRVRSPIVVRLGG
Sbjct: 2179 YLFGKLEWSDGKHRVRSPIVVRLGG 2203

BLAST of HG10003275 vs. NCBI nr
Match: XP_011650462.2 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 1255.4 bits (3247), Expect = 0.0e+00
Identity = 622/749 (83.04%), Postives = 669/749 (89.32%), Query Frame = 0

Query: 6   FCLLSYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGF 65
           F   SYIVYLGSHSHG NPS+ D QIATESH+NLLGSFLGSNE AKEAIFYSY+RHINGF
Sbjct: 17  FFYKSYIVYLGSHSHGFNPSSTDAQIATESHFNLLGSFLGSNEEAKEAIFYSYNRHINGF 76

Query: 66  AAVLDQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGES 125
           AAV+DQKVA DLAKHPDVVSV ENKGRKLHTT SW FLG+EN+GAIPSNS+W+LASFGES
Sbjct: 77  AAVVDQKVAEDLAKHPDVVSVLENKGRKLHTTNSWKFLGLENNGAIPSNSLWNLASFGES 136

Query: 126 TIIGNLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAG 185
           TIIGNLDTGVWPESKSF+D+ YGPIP+RWKGSCEGGSKF+CNRKLIGARY+NKGYAAI G
Sbjct: 137 TIIGNLDTGVWPESKSFSDKEYGPIPSRWKGSCEGGSKFYCNRKLIGARYYNKGYAAIVG 196

Query: 186 PLNSSYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPL 245
           PLNSSYE+ARDHEGHGTHTLSTAGGHFV  AN+FGYGNGTAKGGSPKA VAAYKVCWP +
Sbjct: 197 PLNSSYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPQV 256

Query: 246 LFVGACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSA 305
           LF G CF+ADILAGFEAAI DGVDVLSVSLGG   DF  DSI+IG+FHAVQNGI VVCSA
Sbjct: 257 LFFGECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFAKDSISIGSFHAVQNGIVVVCSA 316

Query: 306 GNSGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLI 365
           GNSGPTPGSVSNVAPWIITVGAST DRL+TSY+ +GDKRH  G S+SDK LP QK YPLI
Sbjct: 317 GNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHFKGASVSDKKLPVQKFYPLI 376

Query: 366 SAVDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILA 425
           S++DAKA N  ++ AL+CEE SLDPKKV GKI+ICLR   ARV KG+VAA+AGAVGMILA
Sbjct: 377 SSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRGDNARVAKGYVAAKAGAVGMILA 436

Query: 426 NDEENGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMAS 485
           N EENGDE+LADAH+LPASHITYSDGQLVYQYINSTKIPMAYMTHVRTE G+KPAPVMAS
Sbjct: 437 NAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTEEGIKPAPVMAS 496

Query: 486 FSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHI 545
           FSSRGPNT+DPSILKPDITAPG NILAAYS D SPTG+  DKRRV FNV+SGTSMSCPH+
Sbjct: 497 FSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGTDFDKRRVPFNVESGTSMSCPHV 556

Query: 546 SGIVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEA 605
           SGIVGLLKTLYP WSPAAIRSAIMTTAGTKANDL PILST Q KANA AYGAGHV PN A
Sbjct: 557 SGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILSTNQEKANAFAYGAGHVSPNRA 616

Query: 606 ANPGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKS 665
           A+PGLVYDLSTKDYLNYLCA+GYN  QIK FSNDTSFVCSKSFK+TD NYPSISIP L+ 
Sbjct: 617 ADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFVCSKSFKLTDLNYPSISIPILEY 676

Query: 666 EAVVNITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPND 725
           +  V I R++KNVGSPGTYVVQV  P GVSVSVEPTSLKFTGIDEEKSFRVV+KS  PN 
Sbjct: 677 DVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSYEPNG 736

Query: 726 FRQKYVFGGLEWSDGIHRVRSPIVVRLGG 755
              KY+FG LEWSDG HRVRSPIVVRLGG
Sbjct: 737 SGPKYLFGKLEWSDGKHRVRSPIVVRLGG 765

BLAST of HG10003275 vs. NCBI nr
Match: TYK19382.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1228.4 bits (3177), Expect = 0.0e+00
Identity = 611/745 (82.01%), Postives = 665/745 (89.26%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SYIVYLGSHSHGLNPS+ID QIATESHYNLLGS LGSNE AKEAIFYSY+RHINGFAAV+
Sbjct: 66  SYIVYLGSHSHGLNPSSIDAQIATESHYNLLGSLLGSNEEAKEAIFYSYNRHINGFAAVV 125

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           DQKVA DLAKHPDVVSV ENKGRKLHTT SW+FLGVE++G IPSNSIW+LASFGESTIIG
Sbjct: 126 DQKVAEDLAKHPDVVSVLENKGRKLHTTNSWSFLGVESNGVIPSNSIWNLASFGESTIIG 185

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAGPLNS 189
           NLDTGVWPE+KSF+D+ YGPIP+RWKGSCEGGS F CNRKLIGARY+NKGYA IAGPLNS
Sbjct: 186 NLDTGVWPEAKSFDDKEYGPIPSRWKGSCEGGSNFQCNRKLIGARYYNKGYAEIAGPLNS 245

Query: 190 SYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLFVG 249
           SYE+ARDHEGHGTHTLSTAGG+FV  AN+FGYGNGTAKGGSPKA VAAYKVCWP L   G
Sbjct: 246 SYESARDHEGHGTHTLSTAGGNFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPKLWLFG 305

Query: 250 ACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGNSG 309
            CF+ADILAGFEAAI+DGVDVLSVSLGG   DF  DSIAIG+FHAVQNGITVVCSAGNSG
Sbjct: 306 ECFDADILAGFEAAIADGVDVLSVSLGGDPSDFGQDSIAIGSFHAVQNGITVVCSAGNSG 365

Query: 310 PTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISAVD 369
           P PG+VSNVAPWIITVGAST DRL+T+Y+ +GDKRH  G SLS+K LP QK YPLI++VD
Sbjct: 366 PAPGTVSNVAPWIITVGASTADRLYTNYVAIGDKRHFKGASLSEKGLPIQKFYPLINSVD 425

Query: 370 AKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILANDEE 429
           AK  N    YA +CE +SLDP+KVKGKIV+CLR   AR +KG+V A+AG VGMILAN EE
Sbjct: 426 AKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEE 485

Query: 430 NGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSR 489
           NGD++ ADAH+LPASHITYSDGQLVYQYINSTKIPMAY+TH RTE GVKPAP+MASFSSR
Sbjct: 486 NGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSR 545

Query: 490 GPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIV 549
           GPN+IDPSILKPDITAPG NILAAYSED SP+G   DKRRV F+V+SGTSMSCPH+SGIV
Sbjct: 546 GPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFDKRRVPFSVESGTSMSCPHVSGIV 605

Query: 550 GLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAANPG 609
           GLLKTLYP WSPAAIRSAIMTTAGTKANDLNPILSTKQ KAN  AYGAGHVRPN+AA+PG
Sbjct: 606 GLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTKQEKANEWAYGAGHVRPNKAADPG 665

Query: 610 LVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEAVV 669
           LVYDLST+DYLNYLCA GY   QIK FSNDTSFVCSKSFKI D NYPSISIP L+S+  +
Sbjct: 666 LVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVCSKSFKIIDLNYPSISIPNLESDVAL 725

Query: 670 NITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFRQK 729
            I RR+KNVGSPGTYVVQVNAP GVSVSVEPTSLKFTGIDEEKSFRVV+KS+VPN F  +
Sbjct: 726 KIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPE 785

Query: 730 YVFGGLEWSDG-IHRVRSPIVVRLG 754
           YVFG LEWSDG  HRVRSPIVV+LG
Sbjct: 786 YVFGKLEWSDGNNHRVRSPIVVKLG 810

BLAST of HG10003275 vs. NCBI nr
Match: XP_016900594.1 (PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo])

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 613/757 (80.98%), Postives = 666/757 (87.98%), Query Frame = 0

Query: 10   SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLG------------SNEAAKEAIFYS 69
            SYIVYLGSHSHGLNPS+ID QIATESHYNLLGS LG            SNE AKEAIFYS
Sbjct: 748  SYIVYLGSHSHGLNPSSIDAQIATESHYNLLGSLLGRYLHSVCCVSKYSNEEAKEAIFYS 807

Query: 70   YDRHINGFAAVLDQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIW 129
            Y+RHINGFAAV+DQKVA DLAKHPDVVSV ENKGRKLHTT SW+FLGVE++G IPSNSIW
Sbjct: 808  YNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLHTTNSWSFLGVESNGVIPSNSIW 867

Query: 130  SLASFGESTIIGNLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFN 189
            +LASFGESTIIGNLDTGVWPE+KSF+D+ YGPIP+RWKGSCEGGS F CNRKLIGARY+N
Sbjct: 868  NLASFGESTIIGNLDTGVWPEAKSFDDKEYGPIPSRWKGSCEGGSNFQCNRKLIGARYYN 927

Query: 190  KGYAAIAGPLNSSYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAA 249
            KGYA IAGPLNSSYE+ARDHEGHGTHTLSTAGG+FV  AN+FGYGNGTAKGGSPKA VAA
Sbjct: 928  KGYAEIAGPLNSSYESARDHEGHGTHTLSTAGGNFVPNANLFGYGNGTAKGGSPKALVAA 987

Query: 250  YKVCWPPLLFVGACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQN 309
            YKVCWP L   G CF+ADILAGFEAAI+DGVDVLSVSLGG   DF  DSIAIG+FHAVQN
Sbjct: 988  YKVCWPKLWLFGECFDADILAGFEAAIADGVDVLSVSLGGDPSDFGQDSIAIGSFHAVQN 1047

Query: 310  GITVVCSAGNSGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILP 369
            GITVVCSAGNSGP PG+VSNVAPWIITVGAST DRL+T+Y+ +GDKRH  G SLS+K LP
Sbjct: 1048 GITVVCSAGNSGPAPGTVSNVAPWIITVGASTADRLYTNYVAIGDKRHFKGASLSEKGLP 1107

Query: 370  EQKLYPLISAVDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEA 429
             QK YPLI++VDAK  N    YA +CE +SLDP+KVKGKIV+CLR   AR +KG+V A+A
Sbjct: 1108 VQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNARTEKGYVVAKA 1167

Query: 430  GAVGMILANDEENGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGV 489
            G VGMILAN EENGD++ ADAH+LPASHITYSDGQLVYQYINSTKIPMAY+TH RTE GV
Sbjct: 1168 GGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMAYLTHPRTESGV 1227

Query: 490  KPAPVMASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSG 549
            KPAP+MASFSSRGPN+IDPSILKPDITAPG NILAAYSED SP+G   DKRRV FNV+SG
Sbjct: 1228 KPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFDKRRVPFNVESG 1287

Query: 550  TSMSCPHISGIVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGA 609
            TSMSCPH+SGIVGLLKTLYP WSPAAIRSAIMTTAGTKANDLNPILSTKQ KAN  AYGA
Sbjct: 1288 TSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTKQEKANEWAYGA 1347

Query: 610  GHVRPNEAANPGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPS 669
            GHVRPN+AA+PGLVYDLST+DYLNYLCA GY   QIK FSNDTSFVCSKSFKITD NYPS
Sbjct: 1348 GHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVCSKSFKITDLNYPS 1407

Query: 670  ISIPELKSEAVVNITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVV 729
            ISIP L+S+  + I RR+KNVGSPGTYVVQVNAP GVSVSVEPTSLKFTGIDEEKSFRVV
Sbjct: 1408 ISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSLKFTGIDEEKSFRVV 1467

Query: 730  VKSSVPNDFRQKYVFGGLEWSDG-IHRVRSPIVVRLG 754
            +KS+VPN F  +YVFG LEWSDG  HRVRSPIVV+LG
Sbjct: 1468 LKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 1504

BLAST of HG10003275 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 1.8e-259
Identity = 454/745 (60.94%), Postives = 553/745 (74.23%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SYIVYLGSH+H    S+  +     SH   L SF+GS+E AKEAIFYSY RHINGFAA+L
Sbjct: 41  SYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAIL 100

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           D+  AA++AKHPDVVSV  NKGRKLHTT SWNF+ +  +G +  +S+W+ A +GE TII 
Sbjct: 101 DENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIA 160

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAG-PLN 189
           NLDTGVWPESKSF+DEGYG +P RWKG C       CNRKLIGARYFNKGY A  G P N
Sbjct: 161 NLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPSN 220

Query: 190 SSYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLFV 249
           +SYET RDH+GHG+HTLSTA G+FV GAN+FG GNGTA GGSPKA VAAYKVCWPP+   
Sbjct: 221 ASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPV--D 280

Query: 250 GA-CFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGN 309
           GA CF+ADILA  EAAI DGVDVLS S+GG + D++ D IAIG+FHAV+NG+TVVCSAGN
Sbjct: 281 GAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGN 340

Query: 310 SGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISA 369
           SGP  G+VSNVAPW+ITVGAS++DR F +++EL + +   GTSLS K LPE+K+Y LISA
Sbjct: 341 SGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISA 400

Query: 370 VDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILAND 429
            DA   N     AL+C++ SLDPKKVKGKI++CLR   ARVDKG  AA AGA GM+L ND
Sbjct: 401 ADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCND 460

Query: 430 EENGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFS 489
           + +G+E+++DAHVLPAS I Y DG+ ++ Y++STK P  Y+      L  KPAP MASFS
Sbjct: 461 KASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFS 520

Query: 490 SRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISG 549
           SRGPNTI P ILKPDITAPGVNI+AA++E T PT    D RR  FN +SGTSMSCPHISG
Sbjct: 521 SRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISG 580

Query: 550 IVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAAN 609
           +VGLLKTL+P WSPAAIRSAIMTT+ T+ N   P++     KAN  +YG+GHV+PN+AA+
Sbjct: 581 VVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAH 640

Query: 610 PGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEA 669
           PGLVYDL+T DYL++LCA GYN T ++LF+ D  + C +   + DFNYPSI++P L    
Sbjct: 641 PGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGS- 700

Query: 670 VVNITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFR 729
            + +TR++KNVG P TY  +   P GV VSVEP  L F    E K F++ ++        
Sbjct: 701 -ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTP-- 760

Query: 730 QKYVFGGLEWSDGIHRVRSPIVVRL 753
             YVFG L W+D  H VRSPIVV+L
Sbjct: 761 SGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of HG10003275 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 8.2e-249
Identity = 438/745 (58.79%), Postives = 543/745 (72.89%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SY+VY G+HSH    +   +    E+HY+ LGSF GS E A +AIFYSY +HINGFAA L
Sbjct: 31  SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           D  +A +++KHP+VVSV  NK  KLHTT SW+FLG+E++  +PS+SIW  A FGE TII 
Sbjct: 91  DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEG--GSKFHCNRKLIGARYFNKGYAAIAGPL 189
           NLDTGVWPESKSF DEG GPIP+RWKG C+    + FHCNRKLIGARYFNKGYAA  G L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210

Query: 190 NSSYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLF 249
           NSS+++ RD +GHG+HTLSTA G FV G +IFG GNGTAKGGSP+A VAAYKVCWPP+  
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVK- 270

Query: 250 VGACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGN 309
              C++AD+LA F+AAI DG DV+SVSLGG    F +DS+AIG+FHA +  I VVCSAGN
Sbjct: 271 GNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGN 330

Query: 310 SGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISA 369
           SGP   +VSNVAPW ITVGAST+DR F S + LG+ +H  G SLS   LP  K YP++++
Sbjct: 331 SGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMAS 390

Query: 370 VDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILAND 429
           V+AKA NA    A +C+  SLDP K KGKI++CLR    RV+KG   A  G +GM+L N 
Sbjct: 391 VNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENT 450

Query: 430 EENGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFS 489
              G++LLAD HVLPA+ +T  D   V +YI+ TK P+A++T  RT+LG+KPAPVMASFS
Sbjct: 451 YVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFS 510

Query: 490 SRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISG 549
           S+GP+ + P ILKPDITAPGV+++AAY+   SPT    D RR+ FN  SGTSMSCPHISG
Sbjct: 511 SKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISG 570

Query: 550 IVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAAN 609
           I GLLKT YP+WSPAAIRSAIMTTA    +   PI +   +KA   ++GAGHV+PN A N
Sbjct: 571 IAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVN 630

Query: 610 PGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEA 669
           PGLVYDL  KDYLN+LC+ GYN +QI +FS +     S    + + NYPSI++P L S +
Sbjct: 631 PGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTS-S 690

Query: 670 VVNITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFR 729
            V ++R VKNVG P  Y V+VN P GV V+V+PTSL FT + E+K+F+V++  S  N   
Sbjct: 691 KVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGN-VA 750

Query: 730 QKYVFGGLEWSDGIHRVRSPIVVRL 753
           + YVFG L WSD  HRVRSPIVV+L
Sbjct: 751 KGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of HG10003275 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 741.5 bits (1913), Expect = 9.4e-213
Identity = 409/764 (53.53%), Postives = 512/764 (67.02%), Query Frame = 0

Query: 11  YIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVLD 70
           YIVY+G+HSHG +P++ D+++AT+SHY+LLGS  GS E AKEAI YSY+RHINGFAA+L+
Sbjct: 32  YIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFAALLE 91

Query: 71  QKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIGN 130
           ++ AAD+AK+P+VVSV  +K  KLHTT SW FLG+   G    NS W    FGE+TIIGN
Sbjct: 92  EEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QNSAWQKGRFGENTIIGN 151

Query: 131 LDTGVWPESKSFNDEGYGPIPTRWKGS-CE-----GGSKFHCNRKLIGARYFNKGYAAIA 190
           +DTGVWPES+SF+D+GYG +P++W+G  C+     G  K  CNRKLIGARY+NK + A  
Sbjct: 152 IDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAHN 211

Query: 191 GPLNSSYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPP 250
           G L+    TARD  GHGTHTLSTAGG+FV GA +F  GNGTAKGGSP+A VAAYKVCW  
Sbjct: 212 GQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCW-S 271

Query: 251 LLFVGACFEADILAGFEAAISDGVDVLSVSLGGG----SQDFVHDSIAIGAFHAVQNGIT 310
           L    +C+ AD+LA  + AI DGVDV++VS G      ++    D I+IGAFHA+   I 
Sbjct: 272 LTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNIL 331

Query: 311 VVCSAGNSGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQK 370
           +V SAGN GPTPG+V+NVAPW+ T+ AST+DR F+S + + ++  I G SL    LP  +
Sbjct: 332 LVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINNQL-IEGASLFVN-LPPNQ 391

Query: 371 LYPLISAVDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRG-IARVDKGFVAAEAGA 430
            + LI + DAK  NA    A +C   +LD  KV GKIV+C R G I  V +G  A  AGA
Sbjct: 392 AFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGA 451

Query: 431 VGMILANDEENGDELLADAHVLPASHITYSDGQLVYQYINSTKI----------PMAYMT 490
            GMIL N  +NG  L A+ HV    +      +     + +T I              M+
Sbjct: 452 RGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMS 511

Query: 491 HVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRR 550
             RT  G KPAPVMASFSSRGPN I PSILKPD+TAPGVNILAAYSE  S +   +D RR
Sbjct: 512 RARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRR 571

Query: 551 -VAFNVDSGTSMSCPHISGIVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILST-KQ 610
              FNV  GTSMSCPH SGI GLLKT +P+WSPAAI+SAIMTTA T  N   PI     +
Sbjct: 572 GFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDK 631

Query: 611 VKANALAYGAGHVRPNEAANPGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKS 670
             A+A AYG+GHVRP+ A  PGLVYDLS  DYLN+LCA GY+Q  I   + + +F+CS S
Sbjct: 632 TLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSGS 691

Query: 671 FKITDFNYPSISIPELKSEAVVNITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTG 730
             + D NYPSI++P L+ +  V I R V NVG P TY V   +P G S++V P SL FT 
Sbjct: 692 HSVNDLNYPSITLPNLRLKP-VTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTK 751

Query: 731 IDEEKSFRVVVKSSVPNDFRQKYVFGGLEWSDGIHRVRSPIVVR 752
           I E K+F+V+V++S     R+KY FG L W+DG H VRSPI V+
Sbjct: 752 IGERKTFKVIVQASSAAT-RRKYEFGDLRWTDGKHIVRSPITVK 787

BLAST of HG10003275 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 4.4e-178
Identity = 360/753 (47.81%), Postives = 479/753 (63.61%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           +YIV++        PS+ D+      H N   S L S   + E + Y+Y+  I+GF+  L
Sbjct: 31  TYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRL 90

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
            Q+ A  L   P V+SV      +LHTT +  FLG++   A     ++  A      ++G
Sbjct: 91  TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA----DLFPEAGSYSDVVVG 150

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFH---CNRKLIGARYFNKGYAAIAGP 189
            LDTGVWPESKS++DEG+GPIP+ WKG CE G+ F    CNRKLIGAR+F +GY +  GP
Sbjct: 151 VLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGP 210

Query: 190 LNSSYE--TARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPP 249
           ++ S E  + RD +GHGTHT STA G  VEGA++ GY +GTA+G +P+A VA YKVCW  
Sbjct: 211 IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW-- 270

Query: 250 LLFVGACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCS 309
              +G CF +DILA  + AI+D V+VLS+SLGGG  D+  D +AIGAF A++ GI V CS
Sbjct: 271 ---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 330

Query: 310 AGNSGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSL-SDKILPEQKLYP 369
           AGN+GP+  S+SNVAPWI TVGA T+DR F +   LG+ ++  G SL   + LP+ KL P
Sbjct: 331 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLP 390

Query: 370 LISAVDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMI 429
            I A    A+NA      +C   +L P+KVKGKIV+C R   ARV KG V   AG VGMI
Sbjct: 391 FIYA--GNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMI 450

Query: 430 LANDEENGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVM 489
           LAN   NG+EL+ADAH+LPA+ +    G ++  Y+ +   P A ++ + T +GVKP+PV+
Sbjct: 451 LANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVV 510

Query: 490 ASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCP 549
           A+FSSRGPN+I P+ILKPD+ APGVNILAA++    PTG + D RRV FN+ SGTSMSCP
Sbjct: 511 AAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCP 570

Query: 550 HISGIVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVK-ANALAYGAGHVRP 609
           H+SG+  LLK+++P WSPAAIRSA+MTTA     D  P+L     K +    +GAGHV P
Sbjct: 571 HVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSP 630

Query: 610 NEAANPGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVC--SKSFKITDFNYPSISI 669
             A NPGL+YDL+T+DYL +LCA  Y   QI+  S   ++ C  SKS+ + D NYPS ++
Sbjct: 631 TTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSFAV 690

Query: 670 PELKSEAVVNITRRVKNVGSPGTYVVQVNA-PPGVSVSVEPTSLKFTGIDEEKSFRV--V 729
             +        TR V +VG  GTY V+V +   GV +SVEP  L F   +E+KS+ V   
Sbjct: 691 -NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT 750

Query: 730 VKSSVPNDFRQKYVFGGLEWSDGIHRVRSPIVV 751
           V SS P+       FG +EWSDG H V SP+ +
Sbjct: 751 VDSSKPSGSNS---FGSIEWSDGKHVVGSPVAI 754

BLAST of HG10003275 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 1.3e-161
Identity = 313/717 (43.65%), Postives = 443/717 (61.79%), Query Frame = 0

Query: 48  EAAKEAIFYSYDRHINGFAAVLDQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVEN 107
           E     I Y+Y    +G AA L Q+ A  L +   VV+V      +LHTT S  FLG+E 
Sbjct: 73  EGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLER 132

Query: 108 DGAIPSNSIWSLASFGESTIIGNLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKF--- 167
                S  +W+        ++G LDTG+WPES+SFND G  P+P  W+G+CE G +F   
Sbjct: 133 Q---ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 192

Query: 168 HCNRKLIGARYFNKGYAAIAGPLNS--SYETARDHEGHGTHTLSTAGGHFVEGANIFGYG 227
           +CNRK++GAR F +GY A  G ++    Y++ RD +GHGTHT +T  G  V+GAN+FG+ 
Sbjct: 193 NCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFA 252

Query: 228 NGTAKGGSPKAHVAAYKVCWPPLLFVGACFEADILAGFEAAISDGVDVLSVSLGGGSQDF 287
            GTA+G + KA VAAYKVCW     VG CF +DIL+  + A++DGV VLS+SLGGG   +
Sbjct: 253 YGTARGMAQKARVAAYKVCW-----VGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTY 312

Query: 288 VHDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGD 347
             DS++I  F A++ G+ V CSAGN GP P S++NV+PWI TVGAST+DR F + +++G 
Sbjct: 313 SRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGT 372

Query: 348 KRHIMGTSL--SDKILPEQKLYPLISAVDAKANNAPEDYALVCEEKSLDPKKVKGKIVIC 407
            R   G SL     +LP+ K YPL   V    N +  D    C + +LD + V GKIVIC
Sbjct: 373 MRTFKGVSLYKGRTVLPKNKQYPL---VYLGRNASSPDPTSFCLDGALDRRHVAGKIVIC 432

Query: 408 LRRGIARVDKGFVAAEAGAVGMILANDEENGDELLADAHVLPASHITYSDGQLVYQYINS 467
            R    RV KG V   AG +GM+L N   NG+EL+AD+H+LPA  +   +G+L+ QY  +
Sbjct: 433 DRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMT 492

Query: 468 TKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSP 527
           +K   A +  + T +G+KP+PV+A+FSSRGPN +   ILKPD+ APGVNILAA++ D +P
Sbjct: 493 SKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAP 552

Query: 528 TGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLN 587
           +  S D RRV FN+ SGTSMSCPH+SG+  L+K+ +P WSPAAI+SA+MTTA    N   
Sbjct: 553 SSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFK 612

Query: 588 PIL-STKQVKANALAYGAGHVRPNEAANPGLVYDLSTKDYLNYLCARGYNQTQIKLFSND 647
           P+  ++    ++   +GAGH+ P  A +PGLVYD+  ++Y  +LC +  + +Q+K+F+  
Sbjct: 613 PLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKH 672

Query: 648 TSFVCSKSFKIT--DFNYPSIS--IPELKSEAVVNITRRVKNVGSP-GTYVVQVNAPPGV 707
           ++  C  +      + NYP+IS   PE      + + R V NVG    +Y V V+   G 
Sbjct: 673 SNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGA 732

Query: 708 SVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFRQKY-VFGGLEWSDGIHRVRSPIVV 751
           SV+V+P +L FT   ++ S+ V  ++     FR K   FGGL W    H+VRSP+++
Sbjct: 733 SVTVQPKTLNFTSKHQKLSYTVTFRTR----FRMKRPEFGGLVWKSTTHKVRSPVII 774

BLAST of HG10003275 vs. ExPASy TrEMBL
Match: A0A0A0L2R9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045120 PE=3 SV=1)

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 621/745 (83.36%), Postives = 668/745 (89.66%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SYIVYLGSHSHG NPS+ D QIATESH+NLLGSFLGSNE AKEAIFYSY+RHINGFAAV+
Sbjct: 16  SYIVYLGSHSHGFNPSSTDAQIATESHFNLLGSFLGSNEEAKEAIFYSYNRHINGFAAVV 75

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           DQKVA DLAKHPDVVSV ENKGRKLHTT SW FLG+EN+GAIPSNS+W+LASFGESTIIG
Sbjct: 76  DQKVAEDLAKHPDVVSVLENKGRKLHTTNSWKFLGLENNGAIPSNSLWNLASFGESTIIG 135

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAGPLNS 189
           NLDTGVWPESKSF+D+ YGPIP+RWKGSCEGGSKF+CNRKLIGARY+NKGYAAI GPLNS
Sbjct: 136 NLDTGVWPESKSFSDKEYGPIPSRWKGSCEGGSKFYCNRKLIGARYYNKGYAAIVGPLNS 195

Query: 190 SYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLFVG 249
           SYE+ARDHEGHGTHTLSTAGGHFV  AN+FGYGNGTAKGGSPKA VAAYKVCWP +LF G
Sbjct: 196 SYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPQVLFFG 255

Query: 250 ACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGNSG 309
            CF+ADILAGFEAAI DGVDVLSVSLGG   DF  DSI+IG+FHAVQNGI VVCSAGNSG
Sbjct: 256 ECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFAKDSISIGSFHAVQNGIVVVCSAGNSG 315

Query: 310 PTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISAVD 369
           PTPGSVSNVAPWIITVGAST DRL+TSY+ +GDKRH  G S+SDK LP QK YPLIS++D
Sbjct: 316 PTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHFKGASVSDKKLPVQKFYPLISSLD 375

Query: 370 AKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILANDEE 429
           AKA N  ++ AL+CEE SLDPKKV GKI+ICLR   ARV KG+VAA+AGAVGMILAN EE
Sbjct: 376 AKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRGDNARVAKGYVAAKAGAVGMILANAEE 435

Query: 430 NGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSR 489
           NGDE+LADAH+LPASHITYSDGQLVYQYINSTKIPMAYMTHVRTE G+KPAPVMASFSSR
Sbjct: 436 NGDEILADAHLLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTEEGIKPAPVMASFSSR 495

Query: 490 GPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIV 549
           GPNT+DPSILKPDITAPG NILAAYS D SPTG+  DKRRV FNV+SGTSMSCPH+SGIV
Sbjct: 496 GPNTVDPSILKPDITAPGENILAAYSRDASPTGTDFDKRRVPFNVESGTSMSCPHVSGIV 555

Query: 550 GLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAANPG 609
           GLLKTLYP WSPAAIRSAIMTTAGTKANDL PILST Q KANA AYGAGHV PN AA+PG
Sbjct: 556 GLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILSTNQEKANAFAYGAGHVSPNRAADPG 615

Query: 610 LVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEAVV 669
           LVYDLSTKDYLNYLCA+GYN  QIK FSNDTSFVCSKSFK+TD NYPSISIP L+ +  V
Sbjct: 616 LVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFVCSKSFKLTDLNYPSISIPILEYDVAV 675

Query: 670 NITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFRQK 729
            I R++KNVGSPGTYVVQV  P GVSVSVEPTSLKFTGIDEEKSFRVV+KS  PN    K
Sbjct: 676 KIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSYEPNGSGPK 735

Query: 730 YVFGGLEWSDGIHRVRSPIVVRLGG 755
           Y+FG LEWSDG HRVRSPIVVRLGG
Sbjct: 736 YLFGKLEWSDGKHRVRSPIVVRLGG 760

BLAST of HG10003275 vs. ExPASy TrEMBL
Match: A0A5D3D773 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00130 PE=3 SV=1)

HSP 1 Score: 1228.4 bits (3177), Expect = 0.0e+00
Identity = 611/745 (82.01%), Postives = 665/745 (89.26%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SYIVYLGSHSHGLNPS+ID QIATESHYNLLGS LGSNE AKEAIFYSY+RHINGFAAV+
Sbjct: 66  SYIVYLGSHSHGLNPSSIDAQIATESHYNLLGSLLGSNEEAKEAIFYSYNRHINGFAAVV 125

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           DQKVA DLAKHPDVVSV ENKGRKLHTT SW+FLGVE++G IPSNSIW+LASFGESTIIG
Sbjct: 126 DQKVAEDLAKHPDVVSVLENKGRKLHTTNSWSFLGVESNGVIPSNSIWNLASFGESTIIG 185

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAGPLNS 189
           NLDTGVWPE+KSF+D+ YGPIP+RWKGSCEGGS F CNRKLIGARY+NKGYA IAGPLNS
Sbjct: 186 NLDTGVWPEAKSFDDKEYGPIPSRWKGSCEGGSNFQCNRKLIGARYYNKGYAEIAGPLNS 245

Query: 190 SYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLFVG 249
           SYE+ARDHEGHGTHTLSTAGG+FV  AN+FGYGNGTAKGGSPKA VAAYKVCWP L   G
Sbjct: 246 SYESARDHEGHGTHTLSTAGGNFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPKLWLFG 305

Query: 250 ACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGNSG 309
            CF+ADILAGFEAAI+DGVDVLSVSLGG   DF  DSIAIG+FHAVQNGITVVCSAGNSG
Sbjct: 306 ECFDADILAGFEAAIADGVDVLSVSLGGDPSDFGQDSIAIGSFHAVQNGITVVCSAGNSG 365

Query: 310 PTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISAVD 369
           P PG+VSNVAPWIITVGAST DRL+T+Y+ +GDKRH  G SLS+K LP QK YPLI++VD
Sbjct: 366 PAPGTVSNVAPWIITVGASTADRLYTNYVAIGDKRHFKGASLSEKGLPIQKFYPLINSVD 425

Query: 370 AKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILANDEE 429
           AK  N    YA +CE +SLDP+KVKGKIV+CLR   AR +KG+V A+AG VGMILAN EE
Sbjct: 426 AKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEE 485

Query: 430 NGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSR 489
           NGD++ ADAH+LPASHITYSDGQLVYQYINSTKIPMAY+TH RTE GVKPAP+MASFSSR
Sbjct: 486 NGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSR 545

Query: 490 GPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIV 549
           GPN+IDPSILKPDITAPG NILAAYSED SP+G   DKRRV F+V+SGTSMSCPH+SGIV
Sbjct: 546 GPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFDKRRVPFSVESGTSMSCPHVSGIV 605

Query: 550 GLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAANPG 609
           GLLKTLYP WSPAAIRSAIMTTAGTKANDLNPILSTKQ KAN  AYGAGHVRPN+AA+PG
Sbjct: 606 GLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTKQEKANEWAYGAGHVRPNKAADPG 665

Query: 610 LVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEAVV 669
           LVYDLST+DYLNYLCA GY   QIK FSNDTSFVCSKSFKI D NYPSISIP L+S+  +
Sbjct: 666 LVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVCSKSFKIIDLNYPSISIPNLESDVAL 725

Query: 670 NITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFRQK 729
            I RR+KNVGSPGTYVVQVNAP GVSVSVEPTSLKFTGIDEEKSFRVV+KS+VPN F  +
Sbjct: 726 KIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPE 785

Query: 730 YVFGGLEWSDG-IHRVRSPIVVRLG 754
           YVFG LEWSDG  HRVRSPIVV+LG
Sbjct: 786 YVFGKLEWSDGNNHRVRSPIVVKLG 810

BLAST of HG10003275 vs. ExPASy TrEMBL
Match: A0A1S4DX85 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=1)

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 613/757 (80.98%), Postives = 666/757 (87.98%), Query Frame = 0

Query: 10   SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLG------------SNEAAKEAIFYS 69
            SYIVYLGSHSHGLNPS+ID QIATESHYNLLGS LG            SNE AKEAIFYS
Sbjct: 748  SYIVYLGSHSHGLNPSSIDAQIATESHYNLLGSLLGRYLHSVCCVSKYSNEEAKEAIFYS 807

Query: 70   YDRHINGFAAVLDQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIW 129
            Y+RHINGFAAV+DQKVA DLAKHPDVVSV ENKGRKLHTT SW+FLGVE++G IPSNSIW
Sbjct: 808  YNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLHTTNSWSFLGVESNGVIPSNSIW 867

Query: 130  SLASFGESTIIGNLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFN 189
            +LASFGESTIIGNLDTGVWPE+KSF+D+ YGPIP+RWKGSCEGGS F CNRKLIGARY+N
Sbjct: 868  NLASFGESTIIGNLDTGVWPEAKSFDDKEYGPIPSRWKGSCEGGSNFQCNRKLIGARYYN 927

Query: 190  KGYAAIAGPLNSSYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAA 249
            KGYA IAGPLNSSYE+ARDHEGHGTHTLSTAGG+FV  AN+FGYGNGTAKGGSPKA VAA
Sbjct: 928  KGYAEIAGPLNSSYESARDHEGHGTHTLSTAGGNFVPNANLFGYGNGTAKGGSPKALVAA 987

Query: 250  YKVCWPPLLFVGACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQN 309
            YKVCWP L   G CF+ADILAGFEAAI+DGVDVLSVSLGG   DF  DSIAIG+FHAVQN
Sbjct: 988  YKVCWPKLWLFGECFDADILAGFEAAIADGVDVLSVSLGGDPSDFGQDSIAIGSFHAVQN 1047

Query: 310  GITVVCSAGNSGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILP 369
            GITVVCSAGNSGP PG+VSNVAPWIITVGAST DRL+T+Y+ +GDKRH  G SLS+K LP
Sbjct: 1048 GITVVCSAGNSGPAPGTVSNVAPWIITVGASTADRLYTNYVAIGDKRHFKGASLSEKGLP 1107

Query: 370  EQKLYPLISAVDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEA 429
             QK YPLI++VDAK  N    YA +CE +SLDP+KVKGKIV+CLR   AR +KG+V A+A
Sbjct: 1108 VQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNARTEKGYVVAKA 1167

Query: 430  GAVGMILANDEENGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGV 489
            G VGMILAN EENGD++ ADAH+LPASHITYSDGQLVYQYINSTKIPMAY+TH RTE GV
Sbjct: 1168 GGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMAYLTHPRTESGV 1227

Query: 490  KPAPVMASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSG 549
            KPAP+MASFSSRGPN+IDPSILKPDITAPG NILAAYSED SP+G   DKRRV FNV+SG
Sbjct: 1228 KPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFDKRRVPFNVESG 1287

Query: 550  TSMSCPHISGIVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGA 609
            TSMSCPH+SGIVGLLKTLYP WSPAAIRSAIMTTAGTKANDLNPILSTKQ KAN  AYGA
Sbjct: 1288 TSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTKQEKANEWAYGA 1347

Query: 610  GHVRPNEAANPGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPS 669
            GHVRPN+AA+PGLVYDLST+DYLNYLCA GY   QIK FSNDTSFVCSKSFKITD NYPS
Sbjct: 1348 GHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVCSKSFKITDLNYPS 1407

Query: 670  ISIPELKSEAVVNITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVV 729
            ISIP L+S+  + I RR+KNVGSPGTYVVQVNAP GVSVSVEPTSLKFTGIDEEKSFRVV
Sbjct: 1408 ISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSLKFTGIDEEKSFRVV 1467

Query: 730  VKSSVPNDFRQKYVFGGLEWSDG-IHRVRSPIVVRLG 754
            +KS+VPN F  +YVFG LEWSDG  HRVRSPIVV+LG
Sbjct: 1468 LKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 1504

BLAST of HG10003275 vs. ExPASy TrEMBL
Match: A0A6J1CQB4 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013335 PE=3 SV=1)

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 579/744 (77.82%), Postives = 652/744 (87.63%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SYI YLGSHSHGLNPSAID+Q+ATESHYNLLGS LGSN AAK+AIFYSY+++INGFAA+L
Sbjct: 29  SYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLGSNVAAKDAIFYSYNKYINGFAAIL 88

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           D+KVA +LAKHP VVSVHENK RKLHTT SW+FLG+ENDG IPSNSIW+LASFGESTIIG
Sbjct: 89  DEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLGLENDGEIPSNSIWNLASFGESTIIG 148

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAGPLNS 189
           NLDTGVWPESKSF+D+GYGPIP+RW+GSCEGGSKF CNRKLIGARYFNKGY A  GPLN+
Sbjct: 149 NLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFRCNRKLIGARYFNKGYVASIGPLNA 208

Query: 190 SYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLFVG 249
           SYETARD EGHGTHTLSTAGG+FV GA++FG GNGTAKGGSP+A VAAY+VCWP +L  G
Sbjct: 209 SYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVAAYRVCWPAVL-TG 268

Query: 250 ACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGNSG 309
            CF ADILAGFEAAI DGVDVLSVSLGG  ++F  D +AIGAFHAVQ+GITVVCSAGNSG
Sbjct: 269 GCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDDGLAIGAFHAVQHGITVVCSAGNSG 328

Query: 310 PTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISAVD 369
           P  G+VSNVAPW+ITVGAST DRLF SY+ LG+++HI G SLSDKILP QK YPLIS  D
Sbjct: 329 PGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKHIKGASLSDKILPAQKFYPLISTAD 388

Query: 370 AKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILANDEE 429
           AKA+N   +YA +CEE SLDPKKV+GKIV+CLR   ARVDKG+VAA+AGAVGMILAN ++
Sbjct: 389 AKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDNARVDKGYVAAQAGAVGMILANAQD 448

Query: 430 NGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSR 489
           NGDELLADAH+LPASH++Y+DG+L++QYI STKIPMAYMTHV+TELGVKPAP MASFSSR
Sbjct: 449 NGDELLADAHLLPASHVSYADGELIFQYIKSTKIPMAYMTHVKTELGVKPAPFMASFSSR 508

Query: 490 GPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIV 549
           GPNTI+ SILKPDI APGV+I+AAYSE+ SP+GSS DKRR  FN +SGTSMSCPH+SGIV
Sbjct: 509 GPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSFDKRRSPFNAESGTSMSCPHVSGIV 568

Query: 550 GLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAANPG 609
           GLLKT YP WSPAAI+SA+MTTA TKANDL+PIL+T Q+KAN L+YGAGHVRPN+A NPG
Sbjct: 569 GLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNTTQLKANPLSYGAGHVRPNKALNPG 628

Query: 610 LVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEAVV 669
           LVYDL+ KDYLN+LCARGYNQTQIK FS+   F CS SFK+TDFNYPSISIP LK    V
Sbjct: 629 LVYDLTLKDYLNFLCARGYNQTQIKKFSS-APFACSHSFKLTDFNYPSISIPNLKF-GPV 688

Query: 670 NITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFRQK 729
              RRVKNVGSPGTYV QV APPGV+VSVEP  LKFTGI EE+SFRVVV+  V N+ R+ 
Sbjct: 689 KAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFTGIGEEQSFRVVVR-RVENEERRG 748

Query: 730 YVFGGLEWSDGIHRVRSPIVVRLG 754
           YVFG L WSDG HRVRSPI V LG
Sbjct: 749 YVFGWLAWSDGNHRVRSPIAVNLG 768

BLAST of HG10003275 vs. ExPASy TrEMBL
Match: A0A6J1L3R2 (subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111500241 PE=3 SV=1)

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 574/744 (77.15%), Postives = 647/744 (86.96%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SYIVYLGSHSHGLNPSA+D+Q+AT++HYNLLGS +GSNEAAKEAIFYSY+RHINGFAAVL
Sbjct: 29  SYIVYLGSHSHGLNPSAVDLQLATQTHYNLLGSVIGSNEAAKEAIFYSYNRHINGFAAVL 88

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           D KVA D+AKHP VVSVHENKGR+LHTT SW+FLGVEN+G +P NS+W+LA+FGESTIIG
Sbjct: 89  DYKVAEDVAKHPSVVSVHENKGRELHTTNSWSFLGVENNGGLPPNSLWNLANFGESTIIG 148

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAGPLNS 189
           NLDTG+WPESKSFNDEGYGPIP RWKGSCEGGS F+CNRKLIGARYFNKGYA+I GPLNS
Sbjct: 149 NLDTGIWPESKSFNDEGYGPIPKRWKGSCEGGSNFNCNRKLIGARYFNKGYASIVGPLNS 208

Query: 190 SYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLFVG 249
           SY TARD +GHGTHTLSTAGGHFV  ANIFG GNGTAKGGSPKA VAAYKVCW P + +G
Sbjct: 209 SYLTARDDQGHGTHTLSTAGGHFVSRANIFGNGNGTAKGGSPKALVAAYKVCW-PRVNIG 268

Query: 250 ACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGNSG 309
            CF++DILAGFEAAISDGVDVLS+SLGG   +F  D IAI +FHAV+NGITVVCSAGNSG
Sbjct: 269 GCFDSDILAGFEAAISDGVDVLSISLGGSPLEFHDDPIAIASFHAVKNGITVVCSAGNSG 328

Query: 310 PTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISAVD 369
           P+PG+V+NVAPW+ITVGASTIDRLFT+Y+ LGDKRHI G S+S+KILP QK YPLI A+D
Sbjct: 329 PSPGTVANVAPWLITVGASTIDRLFTTYLALGDKRHIKGESISNKILPVQKFYPLIRALD 388

Query: 370 AKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILANDEE 429
           AK NN     A++C E SLDPKKV+GKIV+CL   IA VDKG+VAA+AGAVGMIL ND+E
Sbjct: 389 AKNNNVSNIEAILCLEGSLDPKKVEGKIVVCLSGDIANVDKGYVAAQAGAVGMILTNDKE 448

Query: 430 NGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSR 489
           +GD L  +AH+LP SHI Y DG+LVYQ+INSTK P+AYMTHVRTELGVKPAP+MA FSSR
Sbjct: 449 DGDRLEVEAHLLPTSHINYIDGELVYQFINSTKTPIAYMTHVRTELGVKPAPIMARFSSR 508

Query: 490 GPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIV 549
           GPN I+PSILKPDITAPGVNILAA++E+TSPTGSS DKRRV FN+ SGTSMSCPHISGIV
Sbjct: 509 GPNIIEPSILKPDITAPGVNILAAFTEETSPTGSSFDKRRVPFNILSGTSMSCPHISGIV 568

Query: 550 GLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAANPG 609
           GLLKTLYP WSPA ++S IMTTA  KANDLN ILS+ + KAN+ AYGAGHV PN+AANPG
Sbjct: 569 GLLKTLYPKWSPAVMKSTIMTTARIKANDLNSILSSGKEKANSFAYGAGHVNPNKAANPG 628

Query: 610 LVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEAVV 669
           LVYDLST+DYLN+LCARGYN+TQ+KLFSNDTSFVCSK FK+ D NYPSI+I  L SE VV
Sbjct: 629 LVYDLSTEDYLNFLCARGYNKTQMKLFSNDTSFVCSKKFKVADLNYPSITIDNLASE-VV 688

Query: 670 NITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFRQK 729
            I RRVKNVGSP TYV  V APP VSVSVEP +LKFT   EEKSF VV+K  VPND+ + 
Sbjct: 689 KIKRRVKNVGSPATYVANVKAPPRVSVSVEPNTLKFTRTGEEKSFTVVLK-RVPNDYHRG 748

Query: 730 YVFGGLEWSDGIHRVRSPIVVRLG 754
            VFG L WS+G H VRSPI+V LG
Sbjct: 749 SVFGRLAWSNGKHHVRSPILVVLG 769

BLAST of HG10003275 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 896.7 bits (2316), Expect = 1.2e-260
Identity = 454/745 (60.94%), Postives = 553/745 (74.23%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SYIVYLGSH+H    S+  +     SH   L SF+GS+E AKEAIFYSY RHINGFAA+L
Sbjct: 41  SYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAIL 100

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           D+  AA++AKHPDVVSV  NKGRKLHTT SWNF+ +  +G +  +S+W+ A +GE TII 
Sbjct: 101 DENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIA 160

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAIAG-PLN 189
           NLDTGVWPESKSF+DEGYG +P RWKG C       CNRKLIGARYFNKGY A  G P N
Sbjct: 161 NLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPSN 220

Query: 190 SSYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLFV 249
           +SYET RDH+GHG+HTLSTA G+FV GAN+FG GNGTA GGSPKA VAAYKVCWPP+   
Sbjct: 221 ASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPV--D 280

Query: 250 GA-CFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGN 309
           GA CF+ADILA  EAAI DGVDVLS S+GG + D++ D IAIG+FHAV+NG+TVVCSAGN
Sbjct: 281 GAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGN 340

Query: 310 SGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISA 369
           SGP  G+VSNVAPW+ITVGAS++DR F +++EL + +   GTSLS K LPE+K+Y LISA
Sbjct: 341 SGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISA 400

Query: 370 VDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILAND 429
            DA   N     AL+C++ SLDPKKVKGKI++CLR   ARVDKG  AA AGA GM+L ND
Sbjct: 401 ADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCND 460

Query: 430 EENGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFS 489
           + +G+E+++DAHVLPAS I Y DG+ ++ Y++STK P  Y+      L  KPAP MASFS
Sbjct: 461 KASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFS 520

Query: 490 SRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISG 549
           SRGPNTI P ILKPDITAPGVNI+AA++E T PT    D RR  FN +SGTSMSCPHISG
Sbjct: 521 SRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISG 580

Query: 550 IVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAAN 609
           +VGLLKTL+P WSPAAIRSAIMTT+ T+ N   P++     KAN  +YG+GHV+PN+AA+
Sbjct: 581 VVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAH 640

Query: 610 PGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEA 669
           PGLVYDL+T DYL++LCA GYN T ++LF+ D  + C +   + DFNYPSI++P L    
Sbjct: 641 PGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGS- 700

Query: 670 VVNITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFR 729
            + +TR++KNVG P TY  +   P GV VSVEP  L F    E K F++ ++        
Sbjct: 701 -ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTP-- 760

Query: 730 QKYVFGGLEWSDGIHRVRSPIVVRL 753
             YVFG L W+D  H VRSPIVV+L
Sbjct: 761 SGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of HG10003275 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 861.3 bits (2224), Expect = 5.8e-250
Identity = 438/745 (58.79%), Postives = 543/745 (72.89%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           SY+VY G+HSH    +   +    E+HY+ LGSF GS E A +AIFYSY +HINGFAA L
Sbjct: 31  SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
           D  +A +++KHP+VVSV  NK  KLHTT SW+FLG+E++  +PS+SIW  A FGE TII 
Sbjct: 91  DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEG--GSKFHCNRKLIGARYFNKGYAAIAGPL 189
           NLDTGVWPESKSF DEG GPIP+RWKG C+    + FHCNRKLIGARYFNKGYAA  G L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210

Query: 190 NSSYETARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPPLLF 249
           NSS+++ RD +GHG+HTLSTA G FV G +IFG GNGTAKGGSP+A VAAYKVCWPP+  
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVK- 270

Query: 250 VGACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCSAGN 309
              C++AD+LA F+AAI DG DV+SVSLGG    F +DS+AIG+FHA +  I VVCSAGN
Sbjct: 271 GNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGN 330

Query: 310 SGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSLSDKILPEQKLYPLISA 369
           SGP   +VSNVAPW ITVGAST+DR F S + LG+ +H  G SLS   LP  K YP++++
Sbjct: 331 SGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMAS 390

Query: 370 VDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMILAND 429
           V+AKA NA    A +C+  SLDP K KGKI++CLR    RV+KG   A  G +GM+L N 
Sbjct: 391 VNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENT 450

Query: 430 EENGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFS 489
              G++LLAD HVLPA+ +T  D   V +YI+ TK P+A++T  RT+LG+KPAPVMASFS
Sbjct: 451 YVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFS 510

Query: 490 SRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISG 549
           S+GP+ + P ILKPDITAPGV+++AAY+   SPT    D RR+ FN  SGTSMSCPHISG
Sbjct: 511 SKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISG 570

Query: 550 IVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVKANALAYGAGHVRPNEAAN 609
           I GLLKT YP+WSPAAIRSAIMTTA    +   PI +   +KA   ++GAGHV+PN A N
Sbjct: 571 IAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVN 630

Query: 610 PGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVCSKSFKITDFNYPSISIPELKSEA 669
           PGLVYDL  KDYLN+LC+ GYN +QI +FS +     S    + + NYPSI++P L S +
Sbjct: 631 PGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTS-S 690

Query: 670 VVNITRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFR 729
            V ++R VKNVG P  Y V+VN P GV V+V+PTSL FT + E+K+F+V++  S  N   
Sbjct: 691 KVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGN-VA 750

Query: 730 QKYVFGGLEWSDGIHRVRSPIVVRL 753
           + YVFG L WSD  HRVRSPIVV+L
Sbjct: 751 KGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of HG10003275 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 626.3 bits (1614), Expect = 3.1e-179
Identity = 360/753 (47.81%), Postives = 479/753 (63.61%), Query Frame = 0

Query: 10  SYIVYLGSHSHGLNPSAIDIQIATESHYNLLGSFLGSNEAAKEAIFYSYDRHINGFAAVL 69
           +YIV++        PS+ D+      H N   S L S   + E + Y+Y+  I+GF+  L
Sbjct: 31  TYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRL 90

Query: 70  DQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVENDGAIPSNSIWSLASFGESTIIG 129
            Q+ A  L   P V+SV      +LHTT +  FLG++   A     ++  A      ++G
Sbjct: 91  TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA----DLFPEAGSYSDVVVG 150

Query: 130 NLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFH---CNRKLIGARYFNKGYAAIAGP 189
            LDTGVWPESKS++DEG+GPIP+ WKG CE G+ F    CNRKLIGAR+F +GY +  GP
Sbjct: 151 VLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGP 210

Query: 190 LNSSYE--TARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTAKGGSPKAHVAAYKVCWPP 249
           ++ S E  + RD +GHGTHT STA G  VEGA++ GY +GTA+G +P+A VA YKVCW  
Sbjct: 211 IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW-- 270

Query: 250 LLFVGACFEADILAGFEAAISDGVDVLSVSLGGGSQDFVHDSIAIGAFHAVQNGITVVCS 309
              +G CF +DILA  + AI+D V+VLS+SLGGG  D+  D +AIGAF A++ GI V CS
Sbjct: 271 ---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 330

Query: 310 AGNSGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGDKRHIMGTSL-SDKILPEQKLYP 369
           AGN+GP+  S+SNVAPWI TVGA T+DR F +   LG+ ++  G SL   + LP+ KL P
Sbjct: 331 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLP 390

Query: 370 LISAVDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLRRGIARVDKGFVAAEAGAVGMI 429
            I A    A+NA      +C   +L P+KVKGKIV+C R   ARV KG V   AG VGMI
Sbjct: 391 FIYA--GNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMI 450

Query: 430 LANDEENGDELLADAHVLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVM 489
           LAN   NG+EL+ADAH+LPA+ +    G ++  Y+ +   P A ++ + T +GVKP+PV+
Sbjct: 451 LANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVV 510

Query: 490 ASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCP 549
           A+FSSRGPN+I P+ILKPD+ APGVNILAA++    PTG + D RRV FN+ SGTSMSCP
Sbjct: 511 AAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCP 570

Query: 550 HISGIVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPILSTKQVK-ANALAYGAGHVRP 609
           H+SG+  LLK+++P WSPAAIRSA+MTTA     D  P+L     K +    +GAGHV P
Sbjct: 571 HVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSP 630

Query: 610 NEAANPGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTSFVC--SKSFKITDFNYPSISI 669
             A NPGL+YDL+T+DYL +LCA  Y   QI+  S   ++ C  SKS+ + D NYPS ++
Sbjct: 631 TTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSFAV 690

Query: 670 PELKSEAVVNITRRVKNVGSPGTYVVQVNA-PPGVSVSVEPTSLKFTGIDEEKSFRV--V 729
             +        TR V +VG  GTY V+V +   GV +SVEP  L F   +E+KS+ V   
Sbjct: 691 -NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT 750

Query: 730 VKSSVPNDFRQKYVFGGLEWSDGIHRVRSPIVV 751
           V SS P+       FG +EWSDG H V SP+ +
Sbjct: 751 VDSSKPSGSNS---FGSIEWSDGKHVVGSPVAI 754

BLAST of HG10003275 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 571.6 bits (1472), Expect = 9.2e-163
Identity = 313/717 (43.65%), Postives = 443/717 (61.79%), Query Frame = 0

Query: 48  EAAKEAIFYSYDRHINGFAAVLDQKVAADLAKHPDVVSVHENKGRKLHTTLSWNFLGVEN 107
           E     I Y+Y    +G AA L Q+ A  L +   VV+V      +LHTT S  FLG+E 
Sbjct: 73  EGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLER 132

Query: 108 DGAIPSNSIWSLASFGESTIIGNLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKF--- 167
                S  +W+        ++G LDTG+WPES+SFND G  P+P  W+G+CE G +F   
Sbjct: 133 Q---ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 192

Query: 168 HCNRKLIGARYFNKGYAAIAGPLNS--SYETARDHEGHGTHTLSTAGGHFVEGANIFGYG 227
           +CNRK++GAR F +GY A  G ++    Y++ RD +GHGTHT +T  G  V+GAN+FG+ 
Sbjct: 193 NCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFA 252

Query: 228 NGTAKGGSPKAHVAAYKVCWPPLLFVGACFEADILAGFEAAISDGVDVLSVSLGGGSQDF 287
            GTA+G + KA VAAYKVCW     VG CF +DIL+  + A++DGV VLS+SLGGG   +
Sbjct: 253 YGTARGMAQKARVAAYKVCW-----VGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTY 312

Query: 288 VHDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGD 347
             DS++I  F A++ G+ V CSAGN GP P S++NV+PWI TVGAST+DR F + +++G 
Sbjct: 313 SRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGT 372

Query: 348 KRHIMGTSL--SDKILPEQKLYPLISAVDAKANNAPEDYALVCEEKSLDPKKVKGKIVIC 407
            R   G SL     +LP+ K YPL   V    N +  D    C + +LD + V GKIVIC
Sbjct: 373 MRTFKGVSLYKGRTVLPKNKQYPL---VYLGRNASSPDPTSFCLDGALDRRHVAGKIVIC 432

Query: 408 LRRGIARVDKGFVAAEAGAVGMILANDEENGDELLADAHVLPASHITYSDGQLVYQYINS 467
            R    RV KG V   AG +GM+L N   NG+EL+AD+H+LPA  +   +G+L+ QY  +
Sbjct: 433 DRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMT 492

Query: 468 TKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSP 527
           +K   A +  + T +G+KP+PV+A+FSSRGPN +   ILKPD+ APGVNILAA++ D +P
Sbjct: 493 SKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAP 552

Query: 528 TGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLN 587
           +  S D RRV FN+ SGTSMSCPH+SG+  L+K+ +P WSPAAI+SA+MTTA    N   
Sbjct: 553 SSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFK 612

Query: 588 PIL-STKQVKANALAYGAGHVRPNEAANPGLVYDLSTKDYLNYLCARGYNQTQIKLFSND 647
           P+  ++    ++   +GAGH+ P  A +PGLVYD+  ++Y  +LC +  + +Q+K+F+  
Sbjct: 613 PLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKH 672

Query: 648 TSFVCSKSFKIT--DFNYPSIS--IPELKSEAVVNITRRVKNVGSP-GTYVVQVNAPPGV 707
           ++  C  +      + NYP+IS   PE      + + R V NVG    +Y V V+   G 
Sbjct: 673 SNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGA 732

Query: 708 SVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFRQKY-VFGGLEWSDGIHRVRSPIVV 751
           SV+V+P +L FT   ++ S+ V  ++     FR K   FGGL W    H+VRSP+++
Sbjct: 733 SVTVQPKTLNFTSKHQKLSYTVTFRTR----FRMKRPEFGGLVWKSTTHKVRSPVII 774

BLAST of HG10003275 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 564.7 bits (1454), Expect = 1.1e-160
Identity = 320/709 (45.13%), Postives = 428/709 (60.37%), Query Frame = 0

Query: 50  AKEAIFYSYDRHINGFAAVLDQKVAAD-LAKHPDVVSVHENKGRKLHTTLSWNFLGVEND 109
           ++ ++ Y+Y    +GF+A LD   A   L+    ++ + E+    LHTT +  FLG+ ++
Sbjct: 56  SESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSE 115

Query: 110 GAIPSNSIWSLASFGESTIIGNLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFH--- 169
                  +  L S     IIG LDTGVWPES+SF+D     IP++WKG CE GS F    
Sbjct: 116 -----FGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKL 175

Query: 170 CNRKLIGARYFNKGY-AAIAGPLNSSYETA--RDHEGHGTHTLSTAGGHFVEGANIFGYG 229
           CN+KLIGAR F+KG+  A  G  +S  E+   RD +GHGTHT +TA G  V  A+  GY 
Sbjct: 176 CNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 235

Query: 230 NGTAKGGSPKAHVAAYKVCWPPLLFVGACFEADILAGFEAAISDGVDVLSVSLGGGSQDF 289
            GTA+G + +A VA YKVCW        CF +DILA  + AI DGVDVLS+SLGGGS  +
Sbjct: 236 AGTARGMATRARVATYKVCWST-----GCFGSDILAAMDRAILDGVDVLSLSLGGGSAPY 295

Query: 290 VHDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGASTIDRLFTSYIELGD 349
             D+IAIGAF A++ G+ V CSAGNSGPT  SV+NVAPW++TVGA T+DR F ++  LG+
Sbjct: 296 YRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGN 355

Query: 350 KRHIMGTSLSDKILPEQKLYPLISAVDAKANNAPEDYALVCEEKSLDPKKVKGKIVICLR 409
            + + G SL   +    K   L+       N      + +C   SLD   V+GKIV+C R
Sbjct: 356 GKRLTGVSLYSGVGMGTKPLELV------YNKGNSSSSNLCLPGSLDSSIVRGKIVVCDR 415

Query: 410 RGIARVDKGFVAAEAGAVGMILANDEENGDELLADAHVLPASHITYSDGQLVYQYINSTK 469
              ARV+KG V  +AG +GMI+AN   +G+EL+AD+H+LPA  +    G L+ +Y+ S  
Sbjct: 416 GVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDS 475

Query: 470 IPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTG 529
            P A +    T L VKP+PV+A+FSSRGPNT+ P ILKPD+  PGVNILA +S+   PTG
Sbjct: 476 KPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTG 535

Query: 530 SSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPTWSPAAIRSAIMTTAGTKANDLNPI 589
              D RR  FN+ SGTSMSCPHISG+ GLLK  +P WSP+AI+SA+MTTA    N   P+
Sbjct: 536 LDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPL 595

Query: 590 L-STKQVKANALAYGAGHVRPNEAANPGLVYDLSTKDYLNYLCARGYNQTQIKLFSNDTS 649
             +     +N  A+G+GHV P +A +PGLVYD+ST++Y+ +LC+  Y    I       S
Sbjct: 596 HDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPS 655

Query: 650 FVCSKSFKIT-DFNYPSISIPELKSEAVVNITRRVKNVGSPGT-YVVQVNAPPGVSVSVE 709
             CSK F      NYPS S+     + VV  TR V NVG+  + Y V VN  P V +SV+
Sbjct: 656 VNCSKKFSDPGQLNYPSFSV-LFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVK 715

Query: 710 PTSLKFTGIDEEKSFRVVVKSSVPNDFRQKYVFGGLEWSDGIHRVRSPI 749
           P+ L F  + E+K + V   S        K  FG + WS+  H VRSP+
Sbjct: 716 PSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPV 747