HG10003266 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10003266 vs. NCBI nr
Match: XP_038904981.1 (chloride channel protein CLC-f [Benincasa hispida])

HSP 1 Score: 1458.0 bits (3773), Expect = 0.0e+00
Identity = 761/800 (95.12%), Postives = 769/800 (96.12%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAEGEFSDQNRLLRSMEDHNEED DLESQDGNGVHP+RNNSGKRGGFLDLLHHLNRGSSF
Sbjct: 1   MAEGEFSDQNRLLRSMEDHNEEDLDLESQDGNGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTASSSSSSDRHNNFNSPSFHAPTAIDGEI 120
           SGRRLSYKRLD+DNHNVNFNPSSVNIVGRSRTA SSSSSDRHNNFNS S HAPTAIDGEI
Sbjct: 61  SGRRLSYKRLDIDNHNVNFNPSSVNIVGRSRTA-SSSSSDRHNNFNSHSLHAPTAIDGEI 120

Query: 121 DDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180
           DDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQR+
Sbjct: 121 DDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRI 180

Query: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240
           ADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT
Sbjct: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240

Query: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300
           LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF
Sbjct: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300

Query: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLY 360
           FAIETVLRPLRAENSPPFTTAMIILASVISSTVS VLLG QSAFTVPAYDLKSAAELPLY
Sbjct: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSTVLLGTQSAFTVPAYDLKSAAELPLY 360

Query: 361 LILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420
           LILGMLCGAVSVAVTRLVAWFGK FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW
Sbjct: 361 LILGMLCGAVSVAVTRLVAWFGKSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420

Query: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480
           GFTNVEEILHTGNRPSAPGIWLLTQIAAAKV+ATALCKGSGLVGGLYAPSLMIGAAVGAV
Sbjct: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVLATALCKGSGLVGGLYAPSLMIGAAVGAV 480

Query: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540
           FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP
Sbjct: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540

Query: 541 LMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWRY 600
           LM           GAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSE KDGA WRY
Sbjct: 541 LM-----------GAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSEHKDGARWRY 600

Query: 601 DNGDNALELSEVVNPSGHE----DNILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDNQ 660
           DNGDN LELSEVVNPSGHE    DN+LEDLKVSQ MSKN LKVSLS+YLKDALKYMKDNQ
Sbjct: 601 DNGDNDLELSEVVNPSGHESSYKDNLLEDLKVSQAMSKNCLKVSLSIYLKDALKYMKDNQ 660

Query: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGRE 720
           QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFM DSLSVDTCLVSSIYTRGISYRGRE
Sbjct: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMSDSLSVDTCLVSSIYTRGISYRGRE 720

Query: 721 RGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREFVSQR 780
           RGILTCYPDT LA AKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLRE ++QR
Sbjct: 721 RGILTCYPDTTLATAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREVINQR 780

Query: 781 ETVYPSGKEIVAQETVADGH 797
           ETVYPSGKEIVAQETVADGH
Sbjct: 781 ETVYPSGKEIVAQETVADGH 788

BLAST of HG10003266 vs. NCBI nr
Match: XP_008448989.1 (PREDICTED: chloride channel protein CLC-f [Cucumis melo])

HSP 1 Score: 1441.8 bits (3731), Expect = 0.0e+00
Identity = 749/800 (93.62%), Postives = 762/800 (95.25%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAE EFSDQNRLLRSMEDHNEEDHDLESQD NGVHP+RNNSGKRGGFLDLLHHLNRGSSF
Sbjct: 1   MAEEEFSDQNRLLRSMEDHNEEDHDLESQDVNGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTASSSSSSDRHNNFNSPSFHAPTAIDGEI 120
           SGRRLSYKR+DMDNHNVNF+PSSVNIVGRSRTASSSSSSDRHNNFNS S H+PTAIDGEI
Sbjct: 61  SGRRLSYKRIDMDNHNVNFSPSSVNIVGRSRTASSSSSSDRHNNFNSYSLHSPTAIDGEI 120

Query: 121 DDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180
           DDN DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM
Sbjct: 121 DDNGDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180

Query: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240
           ADTWHRILLIPV GGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT
Sbjct: 181 ADTWHRILLIPVCGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240

Query: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300
           LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF
Sbjct: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300

Query: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLY 360
           FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLG QSAFTVP YDLKSAAELPLY
Sbjct: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY 360

Query: 361 LILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420
           LILGMLCGAVSVAVTRLVAWFGK FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW
Sbjct: 361 LILGMLCGAVSVAVTRLVAWFGKSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420

Query: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480
           GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV
Sbjct: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480

Query: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540
           FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP
Sbjct: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540

Query: 541 LMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWRY 600
           LM           GAVGLAIWVPSVTKQTKE ESSDKRG ARGYTSLSPSERKDGA WRY
Sbjct: 541 LM-----------GAVGLAIWVPSVTKQTKENESSDKRGFARGYTSLSPSERKDGASWRY 600

Query: 601 DNGDNALELSEVVNPSGH----EDNILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDNQ 660
           DNG N LELSEVVNPSGH    ED+ILEDL+VSQ MSKNYLKVSLS YLKDALKYMKDNQ
Sbjct: 601 DNGGNDLELSEVVNPSGHESNYEDSILEDLEVSQAMSKNYLKVSLSTYLKDALKYMKDNQ 660

Query: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGRE 720
           QNC LVVDD+DFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTC VSSIYTRGI YRGRE
Sbjct: 661 QNCVLVVDDEDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCFVSSIYTRGIRYRGRE 720

Query: 721 RGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREFVSQR 780
           RGILTCYPDTALA AKELMEAKGIKQLPVVKRGKE+KRRIVA+LHY+S+ SCLREFV+Q+
Sbjct: 721 RGILTCYPDTALATAKELMEAKGIKQLPVVKRGKEKKRRIVAILHYNSLISCLREFVNQK 780

Query: 781 ETVYPSGKEIVAQETVADGH 797
           ETVYPSGKEIV QE VADGH
Sbjct: 781 ETVYPSGKEIVVQENVADGH 789

BLAST of HG10003266 vs. NCBI nr
Match: XP_031739481.1 (chloride channel protein CLC-f [Cucumis sativus] >KGN55984.1 hypothetical protein Csa_010834 [Cucumis sativus])

HSP 1 Score: 1432.2 bits (3706), Expect = 0.0e+00
Identity = 749/800 (93.62%), Postives = 759/800 (94.88%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAE EFSDQNRLLRSMEDHNEEDHDLESQD NGVHP+RNNSGKRGGFLDLLHHLNRGSSF
Sbjct: 1   MAEEEFSDQNRLLRSMEDHNEEDHDLESQDANGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTASSSSSSDRHNNFNSPSFHAPTAIDGEI 120
           SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTA SSSSSDRHNNFNS S H+PTAIDGEI
Sbjct: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTA-SSSSSDRHNNFNSYSLHSPTAIDGEI 120

Query: 121 DDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180
           DDN DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM
Sbjct: 121 DDNGDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180

Query: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240
           ADTWHRILLIPV GGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT
Sbjct: 181 ADTWHRILLIPVCGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240

Query: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300
           LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF
Sbjct: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300

Query: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLY 360
           FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLG QSAFTVP YDLKSAAELPLY
Sbjct: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY 360

Query: 361 LILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420
           LILGMLCGAVSVAVTRLVAWFG  FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW
Sbjct: 361 LILGMLCGAVSVAVTRLVAWFGNSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420

Query: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480
           GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV
Sbjct: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480

Query: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540
           FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP
Sbjct: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540

Query: 541 LMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWRY 600
           LM           GAVGLAIWVPSVTKQTKE ESSDKRGLARGYTSLSPSERKDGA WRY
Sbjct: 541 LM-----------GAVGLAIWVPSVTKQTKENESSDKRGLARGYTSLSPSERKDGASWRY 600

Query: 601 DNGDNALELSEVVNPSGHEDN----ILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDNQ 660
           DNG   LELSEVV+ SGHE N    ILEDLKVSQ MSKNYLKVSLS YLKDALKYMKDNQ
Sbjct: 601 DNGGIDLELSEVVSSSGHESNYEESILEDLKVSQAMSKNYLKVSLSTYLKDALKYMKDNQ 660

Query: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGRE 720
           QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGI YRGRE
Sbjct: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGIRYRGRE 720

Query: 721 RGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREFVSQR 780
           RGILTCYPDTALA AKELMEAKGIKQLPVV RGKE+KRRIVA+L+Y+S+ SCLREFV+Q+
Sbjct: 721 RGILTCYPDTALATAKELMEAKGIKQLPVVMRGKEKKRRIVAILYYNSLISCLREFVNQK 780

Query: 781 ETVYPSGKEIVAQETVADGH 797
           ETVYPSGKEIV QE VADGH
Sbjct: 781 ETVYPSGKEIVVQENVADGH 788

BLAST of HG10003266 vs. NCBI nr
Match: XP_022143471.1 (chloride channel protein CLC-f isoform X1 [Momordica charantia])

HSP 1 Score: 1383.2 bits (3579), Expect = 0.0e+00
Identity = 718/800 (89.75%), Postives = 741/800 (92.62%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAEGEFSDQNRLLRSMEDH+ EDHDLESQDG GVH  RNNSGKRGGFLDL  HLNRG+SF
Sbjct: 1   MAEGEFSDQNRLLRSMEDHSGEDHDLESQDGVGVHFHRNNSGKRGGFLDLFQHLNRGNSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTASSSSSSDRHNNFNSPSFHAPTAIDGEI 120
           SGRRLSYKR++MDNHNVN+NPSSVNI+GRSR A  S+SSDRHNNFNS   HAPTAIDGEI
Sbjct: 61  SGRRLSYKRIEMDNHNVNYNPSSVNIIGRSRAA--STSSDRHNNFNSHPHHAPTAIDGEI 120

Query: 121 DDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180
           DDN+DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM
Sbjct: 121 DDNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180

Query: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240
           ADTWHRILLIPVTGGVIVGMMHGLLEIL QIKQSS+ Q QGFDLLSGVFPTVKAIQAAIT
Sbjct: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILCQIKQSSSPQSQGFDLLSGVFPTVKAIQAAIT 240

Query: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300
           LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKI+IAFVAAGAAAGIASGFNAAVAGSF
Sbjct: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIRIAFVAAGAAAGIASGFNAAVAGSF 300

Query: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLY 360
           FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLG QSAFTVPAYDLKSAAELPLY
Sbjct: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPAYDLKSAAELPLY 360

Query: 361 LILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420
           LILGMLCG VSVAVTRLVAWFGK FEF+KERFGLPPVVCPALGGLGAGIIALKYPGILYW
Sbjct: 361 LILGMLCGVVSVAVTRLVAWFGKSFEFVKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420

Query: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480
           GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA+
Sbjct: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAI 480

Query: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540
           FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP
Sbjct: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540

Query: 541 LMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWRY 600
           LM           GAVGLAIWVPSVTKQTKE ESSDKRG ARGY SLSPSE KDG PWRY
Sbjct: 541 LM-----------GAVGLAIWVPSVTKQTKETESSDKRGTARGYASLSPSEHKDGVPWRY 600

Query: 601 DNGDNALELSEVVNPSGH----EDNILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDNQ 660
           DNGD  LELSEVVNP+ H    EDN+LE LKVSQVMSKNYLKVSLSMY+KD LKYMKDNQ
Sbjct: 601 DNGDGDLELSEVVNPTDHESNYEDNLLEGLKVSQVMSKNYLKVSLSMYVKDVLKYMKDNQ 660

Query: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGRE 720
           QNCALV DD DFLEGILT+ DIKRY FKKYGD+  GD LSVDTCLVSSIYTRGISYRGRE
Sbjct: 661 QNCALVADDGDFLEGILTNRDIKRYFFKKYGDSLKGDPLSVDTCLVSSIYTRGISYRGRE 720

Query: 721 RGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREFVSQR 780
           RGILTCYPDT LA AKELMEAKGIKQLPVVKRG+ERKRRIVA+LHYDSI + LRE V +R
Sbjct: 721 RGILTCYPDTDLATAKELMEAKGIKQLPVVKRGRERKRRIVAILHYDSILNYLREVVDKR 780

Query: 781 ETVYPSGKEIVAQETVADGH 797
           ET YPSGKE+V QE +ADGH
Sbjct: 781 ETAYPSGKELVVQENIADGH 787

BLAST of HG10003266 vs. NCBI nr
Match: XP_023005300.1 (chloride channel protein CLC-f-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1359.7 bits (3518), Expect = 0.0e+00
Identity = 709/801 (88.51%), Postives = 735/801 (91.76%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAE EFSDQNRLLRSMEDH  EDHDLESQDGNG+ P+RNNSGKRGG LD+ HHLNRG SF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGGILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTA-SSSSSSDRHNNFNSPSFHAPTAIDGE 120
           SGRRLS KR DMDNH   F+P+S+NIVGR+RTA SSSSSSDRHNNFNS S HAPT  D E
Sbjct: 61  SGRRLSSKRHDMDNHAATFSPTSLNIVGRTRTASSSSSSSDRHNNFNSYSLHAPTVSDTE 120

Query: 121 IDDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQR 180
           IDDNVDDTA PEWALLLIACLLGLATGLCVAAFN+GVHVIHEWAWAGTPNEGAAWLRLQR
Sbjct: 121 IDDNVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQR 180

Query: 181 MADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAI 240
           MADTWHRILLIPVTGGVIVGMMHGLLEIL QIKQSS SQ QGFDLLSGVFPTVKAIQAAI
Sbjct: 181 MADTWHRILLIPVTGGVIVGMMHGLLEILDQIKQSSTSQRQGFDLLSGVFPTVKAIQAAI 240

Query: 241 TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS 300
           TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS
Sbjct: 241 TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS 300

Query: 301 FFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPL 360
           FFAIETVLRPLRAENSPPFTTAMIIL+SVISSTVS VLLG QSAFTVPAYDLKSAAELPL
Sbjct: 301 FFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELPL 360

Query: 361 YLILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY 420
           YLILGMLCG VSVAVTRLVAWF KLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY
Sbjct: 361 YLILGMLCGVVSVAVTRLVAWFSKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY 420

Query: 421 WGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 480
           WGFTNVEEILHTGNRPSAPG+WLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA
Sbjct: 421 WGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 480

Query: 481 VFGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILL 540
           VFGGSAVE+IN AIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILL
Sbjct: 481 VFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILL 540

Query: 541 PLMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWR 600
           PLM           GAVGLAIWVPSVTKQTKEIESS+KR   RGY+SLSP   KDGA WR
Sbjct: 541 PLM-----------GAVGLAIWVPSVTKQTKEIESSEKRVSTRGYSSLSPPGHKDGASWR 600

Query: 601 YDNGDNALELSEVVNP----SGHEDNILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDN 660
           YD+GD  LELSEVV P    S +EDN+LE LKVS+ MSKNYLKV LS+YLKDALKYMKDN
Sbjct: 601 YDSGDKDLELSEVVTPSDRESNYEDNLLEKLKVSKAMSKNYLKVPLSLYLKDALKYMKDN 660

Query: 661 QQNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGR 720
           QQNCALVVD+DD LEGILT+GDIKRYLFKKYGD    DSLSVDTCLVSSIYTRGISYRGR
Sbjct: 661 QQNCALVVDNDDLLEGILTNGDIKRYLFKKYGDTLKSDSLSVDTCLVSSIYTRGISYRGR 720

Query: 721 ERGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREFVSQ 780
           ERGILTCYPD ALAIAKELMEAKG+KQLPV+KRG+ RKRRIVAVLHYDSIFSCLREF++Q
Sbjct: 721 ERGILTCYPDNALAIAKELMEAKGVKQLPVIKRGRGRKRRIVAVLHYDSIFSCLREFINQ 780

Query: 781 RETVYPSGKEIVAQETVADGH 797
           RE VYPSGKEIV QE+VAD H
Sbjct: 781 REKVYPSGKEIVVQESVADSH 790

BLAST of HG10003266 vs. ExPASy Swiss-Prot
Match: Q8RXR2 (Chloride channel protein CLC-f OS=Arabidopsis thaliana OX=3702 GN=CLC-F PE=2 SV=2)

HSP 1 Score: 966.5 bits (2497), Expect = 1.9e-280
Identity = 537/810 (66.30%), Postives = 624/810 (77.04%), Query Frame = 0

Query: 4   GEFSDQNRLLRSMEDH------NEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRG 63
           GE+++   LLRS +         E D D+ESQ       +R+ +   GG  DL  H++R 
Sbjct: 7   GEYNEDRHLLRSTDGDEVGIGGGEGDLDVESQS----PAIRSGA---GGVRDLFKHIDRR 66

Query: 64  SSFSGRRLSYKRLDMDNHNVN---FNPSSVNIVGRSRTASSSSSSDRHNNFNSPSFHAPT 123
            S SGRRLS+KR  M+N  V+    NPSS      S   S++   D     N  S     
Sbjct: 67  FSLSGRRLSFKR--MENIRVDRERHNPSS------SSAFSAAGEEDGGGISNLHSVDDRN 126

Query: 124 AIDGEIDDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAW 183
              G  ++ + D+APPEWALLLI CL+G+A G+CVA FN GVHVIHEWAWAGTPNEGAAW
Sbjct: 127 DEYGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAW 186

Query: 184 LRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKA 243
           LRLQR+ADTWHRILLIPVTGGVIVGMMHGLLEIL QI+QS++SQ QG D L+G++P +KA
Sbjct: 187 LRLQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGLDFLAGIYPVIKA 246

Query: 244 IQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNA 303
           IQAA+TLGTGCSLGPEGPSVDIGKSCANGF LMMENN E+ +IA  AAGAA+GIASGFNA
Sbjct: 247 IQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRER-RIALTAAGAASGIASGFNA 306

Query: 304 AVAGSFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSA 363
           AVAG FFAIETVLRPLRAENSPPFTTAMIILASVISSTVSN LLG QSAFTVP+YDLKSA
Sbjct: 307 AVAGCFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNALLGTQSAFTVPSYDLKSA 366

Query: 364 AELPLYLILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKY 423
           AELPLYLILGMLCGAVSV  +RLV WF K F+FIK++FGLP +VCPALGGLGAGIIALKY
Sbjct: 367 AELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALKY 426

Query: 424 PGILYWGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIG 483
           PGILYWGFTNVEEILHTG   SAPGIWLL Q+AAAKVVATALCKGSGLVGGLYAPSLMIG
Sbjct: 427 PGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMIG 486

Query: 484 AAVGAVFGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKD 543
           AAVGAVFGGSA EIIN AIPGNAAVAQPQAYALVGMAATLAS+CSVPLTSVLLLFELTKD
Sbjct: 487 AAVGAVFGGSAAEIINRAIPGNAAVAQPQAYALVGMAATLASMCSVPLTSVLLLFELTKD 546

Query: 544 YRILLPLMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKD 603
           YRILLPLM           GAVGLAIWVPSV  Q KE +SS+ R   RGY+SLSPSERK 
Sbjct: 547 YRILLPLM-----------GAVGLAIWVPSVANQGKESDSSEGRSTGRGYSSLSPSERKT 606

Query: 604 GAPWRYDNGDNALELSEVVNPSGH----EDNILEDLKVSQVMSKNYLKVSLSMYLKDALK 663
              WR+ +  ++LEL+ + NP  +    E+ ILEDLKV +VMSKNY+KVS    L++A  
Sbjct: 607 EGVWRHTDNADSLELTVIENPDHNSFLDEETILEDLKVMRVMSKNYVKVSSGTTLREARN 666

Query: 664 YMKDNQQNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGI 723
            +K++ QNC +VVDDDDFL GILTHGDI+RYL            L  +TC VSS+ T+ I
Sbjct: 667 ILKESHQNCIMVVDDDDFLAGILTHGDIRRYLSNN-----ASTILDENTCPVSSVCTKKI 726

Query: 724 SYRGRERGILTCYPDTALAIAKELMEAKGIKQLPVVKRG----KERKRRIVAVLHYDSIF 783
           SYRG+ERG+LTCYPD  + +AKELMEA+G+KQLPVVKRG    K ++R+++ +LHYDSI+
Sbjct: 727 SYRGQERGLLTCYPDATVGVAKELMEARGVKQLPVVKRGEVIHKGKRRKLLGLLHYDSIW 781

Query: 784 SCLREFVSQRETVYPSGKEIVAQETVADGH 797
           + LR+ +S+R ++    K+   +E   +GH
Sbjct: 787 TFLRDEMSRRRSINDRRKD---KEVGTNGH 781

BLAST of HG10003266 vs. ExPASy Swiss-Prot
Match: Q8GX93 (Chloride channel protein CLC-e OS=Arabidopsis thaliana OX=3702 GN=CLC-E PE=2 SV=2)

HSP 1 Score: 397.9 bits (1021), Expect = 2.7e-109
Identity = 258/638 (40.44%), Postives = 370/638 (57.99%), Query Frame = 0

Query: 126 DTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMADTWH 185
           D  P +   +  ACL+G+ TG+ V  FN  VH++ +++W G P+ GA+WLR   +   W 
Sbjct: 68  DQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRGASWLREAPIGSNWL 127

Query: 186 RILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVF-PTVKAIQAAITLGTG 245
           R++L+P  GG++V +++ L E      +S+       D +  V  P +K + A +TLGTG
Sbjct: 128 RVILVPTIGGLVVSILNQLRE---SAGKSTGDSHSSLDRVKAVLRPFLKTVAACVTLGTG 187

Query: 246 CSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIE 305
            SLGPEGPSV+IG S A G   +  N S +   + +AAG+AAGI+SGFNAAVAG FFA+E
Sbjct: 188 NSLGPEGPSVEIGASIAKGVNSLF-NKSPQTGFSLLAAGSAAGISSGFNAAVAGCFFAVE 247

Query: 306 TVLRPLRAENSP---PFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLYL 365
           +VL P  + +S    P TT+M+IL++V +S VS + LG++ AF VP YD +S  ELPLYL
Sbjct: 248 SVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFRSPGELPLYL 307

Query: 366 ILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 425
           +LG LCG VS+A++R  +      + + +  G+P  V P +GGL  GIIAL YP +LYWG
Sbjct: 308 LLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIALVYPEVLYWG 367

Query: 426 FTNVEEILHTGNRPSAPGIW--LLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 485
           F NV+ +L    RP   G+   LL Q+ A K+ ATA C+ SGLVGG YAPSL IG A G 
Sbjct: 368 FQNVDILLE--KRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLFIGGAAGM 427

Query: 486 VFGGSAVEIINIAIPGN-------AAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELT 545
            +G    + I +A+  N         VA PQAY LVGMAATLA VC VPLT+VLLLFELT
Sbjct: 428 AYG----KFIGLALAQNPDFNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELT 487

Query: 546 KDYRILLPLMAIEKLDTTFPWGAVGLAIWV---PSVTKQTKEIESSDKRGLARGYTSLSP 605
           +DYRI+LPL+           GAVG++ W+    S  ++T+E + + KR       SL+ 
Sbjct: 488 QDYRIVLPLL-----------GAVGMSSWITSGQSKRQETRETKETRKRKSQEAVQSLTS 547

Query: 606 SERKDGAPWRYDNGDNALELSEVVNPSGHEDNILEDLKVSQVMSKNYLKVSLSMYLKDAL 665
           S+  + +       +++L L + +N S   + + + + VS+ M   +  V +S  L++AL
Sbjct: 548 SD-DESSTNNLCEVESSLCLDDSLNQS---EELPKSIFVSEAMRTRFATVMMSTSLEEAL 607

Query: 666 KYMKDNQQNCALVVDDDDFLEGILTHGDIKRY-LFKKYGDAFMGDSLSVDTCLVSSIYTR 725
             M   +Q+CAL+VD D+   GILT  DI+ +   +K G+    D    D C  S     
Sbjct: 608 TRMLIEKQSCALIVDPDNIFLGILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSG---- 667

Query: 726 GISYRGRERGILTCYPDTALAIAKELMEAKGIKQLPVV 747
                G+ +   T  PD  L  A+ +M    +  + VV
Sbjct: 668 -----GKCKVPWTVTPDMDLLAAQTIMNKHELSHVAVV 671

BLAST of HG10003266 vs. ExPASy Swiss-Prot
Match: Q8XTT4 (Putative chloride channel protein ClcB-like OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=RSp0020 PE=3 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 8.3e-34
Identity = 131/417 (31.41%), Postives = 207/417 (49.64%), Query Frame = 0

Query: 134 LLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMADTWHRILLIPVT 193
           +L IA L+G A  L   AF   +  + +W  AG      A  R    A  W   LL+P  
Sbjct: 1   MLAIAGLIGCAGALATIAFRECLRQL-QWWLAGADQGLVATAR----ALPWWARLLVPTA 60

Query: 194 GGVIVG--MMHGLLEILSQ-IKQSSASQGQGFDLLSGVFPTVKAIQAAITLGTGCSLGPE 253
           GG++ G  + +GL  I  +  +    +   G  +LS     V++  +  ++ +G S+G E
Sbjct: 61  GGLLAGLTLQYGLKWIPRKGAEDYMEAIAVGDGVLSARQSLVRSASSLCSVASGASIGRE 120

Query: 254 GPSVDIGKSCAN--GFYL--MMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIETV 313
           GP V +   C +  G  L   M  + E +++  VA GAAAGI S +NA +AG+ F  E V
Sbjct: 121 GPMVQLAAMCGSLLGRVLRHAMPVSVEHMRL-LVACGAAAGITSAYNAPIAGAVFVCEIV 180

Query: 314 LRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLYLILGML 373
              +      P     +++++V +  +     G  + + +P +D  S  E+  YL LG+ 
Sbjct: 181 FGAITTATLGP-----LLVSAVTADIIVRQFFGYGAVYAMPHFDFVSGWEVLTYLGLGLA 240

Query: 374 CGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWGFTNVE 433
            G     +  L+      F     R  LP  +  ALGGL  G ++++ P +   G++ V 
Sbjct: 241 AGMAGPLLLGLIDRARGAF----ARTRLPQALRLALGGLIVGALSIRVPEVWGNGYSVVN 300

Query: 434 EILHTGNRPSAPGIW-LLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSA 493
             LH      AP +W  +  +   KV ATA   GSG VGG++ P+L  GAA+G ++G   
Sbjct: 301 GFLH------APWLWQTVALVLVCKVGATAASAGSGAVGGVFTPTLFCGAALGLLYGTG- 360

Query: 494 VEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLPLM 543
              ++  +PG A V  P +YA+VGM A LA+    PL S+L++FE+T  Y+++LPLM
Sbjct: 361 ---MHALLPGAAPV--PVSYAVVGMGALLAATTHAPLMSILMIFEMTLSYQVVLPLM 390

BLAST of HG10003266 vs. ExPASy Swiss-Prot
Match: A7N6K9 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio campbellii (strain ATCC BAA-1116 / BB120) OX=338187 GN=clcA PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 7.5e-27
Identity = 117/438 (26.71%), Postives = 207/438 (47.26%), Query Frame = 0

Query: 127 TAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLR--LQRMADTW 186
           + P  +++L +A ++G   GL    F I VH + E     T  E   WL+  +  +   W
Sbjct: 28  STPTSFSVLFMAAIVGTLAGLVGTYFEIAVHFVSE-----TRTE---WLKSEIGSVLPLW 87

Query: 187 HRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQ-------- 246
              +LI    G +  + + L+      + +  + G G   + G    +++++        
Sbjct: 88  LAAILI---SGALAFIGYYLVN-----RFAPEASGSGIPEIEGAMDNIRSVRWWRVIPVK 147

Query: 247 ---AAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFN 306
                  LG+G  LG EGP+V +G +       +     +  + + +A+GAA G+A+ FN
Sbjct: 148 FFGGMGALGSGMVLGREGPTVQMGGAVGRMVTDIFRVKDDDTRHSLLASGAAGGLAAAFN 207

Query: 307 AAVAGSFFAIETVLRPLRAENSPPFTTAMI-ILASVISSTVSNVLL----GAQSAFTVPA 366
           A +A   F +E        E  P F  ++I I A +IS+ ++N++     G ++  T+P 
Sbjct: 208 APLAAIMFVVE--------EMRPQFRYSLISIRAVIISAIMANIVFRAINGQEAVITMPQ 267

Query: 367 YDLKSAAELPLYLILGMLCGAVSVAVTRLVA----WFGKLFEFIKERFGLPPVVCPALGG 426
           Y       L L+L+LG L G   V   +L+      F  L +  ++R+ +   +   LGG
Sbjct: 268 YQSPELQSLWLFLLLGSLFGVFGVVFNKLITIAQDSFVALHKNDRKRYLITGTI---LGG 327

Query: 427 LGAGIIALKYPGILYWGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVG 486
           +  G++ L  P +   G   + +I + GN      I +L  +   +V+ T LC GSG  G
Sbjct: 328 V-FGLLLLYVPQLTGGGIGLIPDITN-GNY----SISILVMLFVGRVITTLLCFGSGAPG 387

Query: 487 GLYAPSLMIGAAVGAVFGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTS 543
           G++AP L +G   G  FG SA    ++ +P  +   +P  +A+ GM A  A+    P+T 
Sbjct: 388 GIFAPMLALGTLFGYAFGASA----DMLLP--SLTIEPGVFAIAGMGALFAATVRAPITG 426

BLAST of HG10003266 vs. ExPASy Swiss-Prot
Match: Q87GZ9 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=clcA PE=3 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.1e-25
Identity = 111/428 (25.93%), Postives = 198/428 (46.26%), Query Frame = 0

Query: 132 WALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMADTWHRILLIP 191
           +++L++A ++G   G     F + VH + E     T  E   WLR +  +       ++P
Sbjct: 31  FSVLIMAAIVGTLAGFVGTYFELAVHFVSE-----TRTE---WLRSEIGS-------VLP 90

Query: 192 V-TGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQ-----------AAI 251
           +    V++  +   +      + +  + G G   + G    ++ ++              
Sbjct: 91  LWLAAVLISALLAFIGYFLVHRFAPEAAGSGIPEIEGAMDNIRPVRWWRVLPVKFFGGMG 150

Query: 252 TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS 311
            LG+G  LG EGP+V +G +       +     +  + + +A+GAA G+A+ FNA +AG 
Sbjct: 151 ALGSGMVLGREGPTVQMGGAVGRMVTDIFRVKDDDTRHSLLASGAAGGLAAAFNAPLAGI 210

Query: 312 FFAIETVLRPLRAENSPPFTTAMI-ILASVISSTVSNVLL----GAQSAFTVPAYDLKSA 371
            F +E        E  P F  ++I I A +IS+ ++N++     G  +  T+P Y   + 
Sbjct: 211 MFVVE--------EMRPQFRYSLISIRAVIISAIMANIVFRAINGQDAVITMPQYQSPAL 270

Query: 372 AELPLYLILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKY 431
             L L+L+LG L G   V   +L+      F  I +      ++  ++ G   G++ L  
Sbjct: 271 QTLWLFLLLGALFGVFGVIFNKLITVAQDSFVAIHKNDRKRYLITGSILGGVFGLLLLYV 330

Query: 432 PGILYWGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIG 491
           P +   G   + ++  TGN      I +L  +   +VV T LC GSG  GG++AP L +G
Sbjct: 331 PQLTGGGIALIPDVT-TGNY----SISILVLLFIGRVVTTLLCFGSGAPGGIFAPMLALG 390

Query: 492 AAVGAVFGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKD 543
              G  FG SA    ++ +P      +P  +A+ GM A  A+    P+T +LL+ E+T +
Sbjct: 391 TLFGYAFGASA----DVLLP--TLDIEPGVFAIAGMGALFAATVRAPITGILLVIEMTNN 424

BLAST of HG10003266 vs. ExPASy TrEMBL
Match: A0A1S3BLN3 (Chloride channel protein OS=Cucumis melo OX=3656 GN=LOC103490996 PE=3 SV=1)

HSP 1 Score: 1441.8 bits (3731), Expect = 0.0e+00
Identity = 749/800 (93.62%), Postives = 762/800 (95.25%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAE EFSDQNRLLRSMEDHNEEDHDLESQD NGVHP+RNNSGKRGGFLDLLHHLNRGSSF
Sbjct: 1   MAEEEFSDQNRLLRSMEDHNEEDHDLESQDVNGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTASSSSSSDRHNNFNSPSFHAPTAIDGEI 120
           SGRRLSYKR+DMDNHNVNF+PSSVNIVGRSRTASSSSSSDRHNNFNS S H+PTAIDGEI
Sbjct: 61  SGRRLSYKRIDMDNHNVNFSPSSVNIVGRSRTASSSSSSDRHNNFNSYSLHSPTAIDGEI 120

Query: 121 DDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180
           DDN DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM
Sbjct: 121 DDNGDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180

Query: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240
           ADTWHRILLIPV GGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT
Sbjct: 181 ADTWHRILLIPVCGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240

Query: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300
           LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF
Sbjct: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300

Query: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLY 360
           FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLG QSAFTVP YDLKSAAELPLY
Sbjct: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY 360

Query: 361 LILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420
           LILGMLCGAVSVAVTRLVAWFGK FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW
Sbjct: 361 LILGMLCGAVSVAVTRLVAWFGKSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420

Query: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480
           GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV
Sbjct: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480

Query: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540
           FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP
Sbjct: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540

Query: 541 LMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWRY 600
           LM           GAVGLAIWVPSVTKQTKE ESSDKRG ARGYTSLSPSERKDGA WRY
Sbjct: 541 LM-----------GAVGLAIWVPSVTKQTKENESSDKRGFARGYTSLSPSERKDGASWRY 600

Query: 601 DNGDNALELSEVVNPSGH----EDNILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDNQ 660
           DNG N LELSEVVNPSGH    ED+ILEDL+VSQ MSKNYLKVSLS YLKDALKYMKDNQ
Sbjct: 601 DNGGNDLELSEVVNPSGHESNYEDSILEDLEVSQAMSKNYLKVSLSTYLKDALKYMKDNQ 660

Query: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGRE 720
           QNC LVVDD+DFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTC VSSIYTRGI YRGRE
Sbjct: 661 QNCVLVVDDEDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCFVSSIYTRGIRYRGRE 720

Query: 721 RGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREFVSQR 780
           RGILTCYPDTALA AKELMEAKGIKQLPVVKRGKE+KRRIVA+LHY+S+ SCLREFV+Q+
Sbjct: 721 RGILTCYPDTALATAKELMEAKGIKQLPVVKRGKEKKRRIVAILHYNSLISCLREFVNQK 780

Query: 781 ETVYPSGKEIVAQETVADGH 797
           ETVYPSGKEIV QE VADGH
Sbjct: 781 ETVYPSGKEIVVQENVADGH 789

BLAST of HG10003266 vs. ExPASy TrEMBL
Match: A0A0A0L4N0 (Chloride channel protein OS=Cucumis sativus OX=3659 GN=Csa_3G044490 PE=3 SV=1)

HSP 1 Score: 1432.2 bits (3706), Expect = 0.0e+00
Identity = 749/800 (93.62%), Postives = 759/800 (94.88%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAE EFSDQNRLLRSMEDHNEEDHDLESQD NGVHP+RNNSGKRGGFLDLLHHLNRGSSF
Sbjct: 1   MAEEEFSDQNRLLRSMEDHNEEDHDLESQDANGVHPLRNNSGKRGGFLDLLHHLNRGSSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTASSSSSSDRHNNFNSPSFHAPTAIDGEI 120
           SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTA SSSSSDRHNNFNS S H+PTAIDGEI
Sbjct: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTA-SSSSSDRHNNFNSYSLHSPTAIDGEI 120

Query: 121 DDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180
           DDN DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM
Sbjct: 121 DDNGDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180

Query: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240
           ADTWHRILLIPV GGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT
Sbjct: 181 ADTWHRILLIPVCGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240

Query: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300
           LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF
Sbjct: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300

Query: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLY 360
           FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLG QSAFTVP YDLKSAAELPLY
Sbjct: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPTYDLKSAAELPLY 360

Query: 361 LILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420
           LILGMLCGAVSVAVTRLVAWFG  FEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW
Sbjct: 361 LILGMLCGAVSVAVTRLVAWFGNSFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420

Query: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480
           GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV
Sbjct: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480

Query: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540
           FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP
Sbjct: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540

Query: 541 LMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWRY 600
           LM           GAVGLAIWVPSVTKQTKE ESSDKRGLARGYTSLSPSERKDGA WRY
Sbjct: 541 LM-----------GAVGLAIWVPSVTKQTKENESSDKRGLARGYTSLSPSERKDGASWRY 600

Query: 601 DNGDNALELSEVVNPSGHEDN----ILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDNQ 660
           DNG   LELSEVV+ SGHE N    ILEDLKVSQ MSKNYLKVSLS YLKDALKYMKDNQ
Sbjct: 601 DNGGIDLELSEVVSSSGHESNYEESILEDLKVSQAMSKNYLKVSLSTYLKDALKYMKDNQ 660

Query: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGRE 720
           QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGI YRGRE
Sbjct: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGIRYRGRE 720

Query: 721 RGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREFVSQR 780
           RGILTCYPDTALA AKELMEAKGIKQLPVV RGKE+KRRIVA+L+Y+S+ SCLREFV+Q+
Sbjct: 721 RGILTCYPDTALATAKELMEAKGIKQLPVVMRGKEKKRRIVAILYYNSLISCLREFVNQK 780

Query: 781 ETVYPSGKEIVAQETVADGH 797
           ETVYPSGKEIV QE VADGH
Sbjct: 781 ETVYPSGKEIVVQENVADGH 788

BLAST of HG10003266 vs. ExPASy TrEMBL
Match: A0A6J1CQC2 (Chloride channel protein OS=Momordica charantia OX=3673 GN=LOC111013347 PE=3 SV=1)

HSP 1 Score: 1383.2 bits (3579), Expect = 0.0e+00
Identity = 718/800 (89.75%), Postives = 741/800 (92.62%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAEGEFSDQNRLLRSMEDH+ EDHDLESQDG GVH  RNNSGKRGGFLDL  HLNRG+SF
Sbjct: 1   MAEGEFSDQNRLLRSMEDHSGEDHDLESQDGVGVHFHRNNSGKRGGFLDLFQHLNRGNSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTASSSSSSDRHNNFNSPSFHAPTAIDGEI 120
           SGRRLSYKR++MDNHNVN+NPSSVNI+GRSR A  S+SSDRHNNFNS   HAPTAIDGEI
Sbjct: 61  SGRRLSYKRIEMDNHNVNYNPSSVNIIGRSRAA--STSSDRHNNFNSHPHHAPTAIDGEI 120

Query: 121 DDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180
           DDN+DDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM
Sbjct: 121 DDNLDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRM 180

Query: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAIT 240
           ADTWHRILLIPVTGGVIVGMMHGLLEIL QIKQSS+ Q QGFDLLSGVFPTVKAIQAAIT
Sbjct: 181 ADTWHRILLIPVTGGVIVGMMHGLLEILCQIKQSSSPQSQGFDLLSGVFPTVKAIQAAIT 240

Query: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSF 300
           LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKI+IAFVAAGAAAGIASGFNAAVAGSF
Sbjct: 241 LGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIRIAFVAAGAAAGIASGFNAAVAGSF 300

Query: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLY 360
           FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLG QSAFTVPAYDLKSAAELPLY
Sbjct: 301 FAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGTQSAFTVPAYDLKSAAELPLY 360

Query: 361 LILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420
           LILGMLCG VSVAVTRLVAWFGK FEF+KERFGLPPVVCPALGGLGAGIIALKYPGILYW
Sbjct: 361 LILGMLCGVVSVAVTRLVAWFGKSFEFVKERFGLPPVVCPALGGLGAGIIALKYPGILYW 420

Query: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAV 480
           GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA+
Sbjct: 421 GFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAI 480

Query: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540
           FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP
Sbjct: 481 FGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLP 540

Query: 541 LMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWRY 600
           LM           GAVGLAIWVPSVTKQTKE ESSDKRG ARGY SLSPSE KDG PWRY
Sbjct: 541 LM-----------GAVGLAIWVPSVTKQTKETESSDKRGTARGYASLSPSEHKDGVPWRY 600

Query: 601 DNGDNALELSEVVNPSGH----EDNILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDNQ 660
           DNGD  LELSEVVNP+ H    EDN+LE LKVSQVMSKNYLKVSLSMY+KD LKYMKDNQ
Sbjct: 601 DNGDGDLELSEVVNPTDHESNYEDNLLEGLKVSQVMSKNYLKVSLSMYVKDVLKYMKDNQ 660

Query: 661 QNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGRE 720
           QNCALV DD DFLEGILT+ DIKRY FKKYGD+  GD LSVDTCLVSSIYTRGISYRGRE
Sbjct: 661 QNCALVADDGDFLEGILTNRDIKRYFFKKYGDSLKGDPLSVDTCLVSSIYTRGISYRGRE 720

Query: 721 RGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREFVSQR 780
           RGILTCYPDT LA AKELMEAKGIKQLPVVKRG+ERKRRIVA+LHYDSI + LRE V +R
Sbjct: 721 RGILTCYPDTDLATAKELMEAKGIKQLPVVKRGRERKRRIVAILHYDSILNYLREVVDKR 780

Query: 781 ETVYPSGKEIVAQETVADGH 797
           ET YPSGKE+V QE +ADGH
Sbjct: 781 ETAYPSGKELVVQENIADGH 787

BLAST of HG10003266 vs. ExPASy TrEMBL
Match: A0A6J1KX22 (Chloride channel protein OS=Cucurbita maxima OX=3661 GN=LOC111498341 PE=3 SV=1)

HSP 1 Score: 1359.7 bits (3518), Expect = 0.0e+00
Identity = 709/801 (88.51%), Postives = 735/801 (91.76%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAE EFSDQNRLLRSMEDH  EDHDLESQDGNG+ P+RNNSGKRGG LD+ HHLNRG SF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGGILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTA-SSSSSSDRHNNFNSPSFHAPTAIDGE 120
           SGRRLS KR DMDNH   F+P+S+NIVGR+RTA SSSSSSDRHNNFNS S HAPT  D E
Sbjct: 61  SGRRLSSKRHDMDNHAATFSPTSLNIVGRTRTASSSSSSSDRHNNFNSYSLHAPTVSDTE 120

Query: 121 IDDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQR 180
           IDDNVDDTA PEWALLLIACLLGLATGLCVAAFN+GVHVIHEWAWAGTPNEGAAWLRLQR
Sbjct: 121 IDDNVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQR 180

Query: 181 MADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAI 240
           MADTWHRILLIPVTGGVIVGMMHGLLEIL QIKQSS SQ QGFDLLSGVFPTVKAIQAAI
Sbjct: 181 MADTWHRILLIPVTGGVIVGMMHGLLEILDQIKQSSTSQRQGFDLLSGVFPTVKAIQAAI 240

Query: 241 TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS 300
           TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS
Sbjct: 241 TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS 300

Query: 301 FFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPL 360
           FFAIETVLRPLRAENSPPFTTAMIIL+SVISSTVS VLLG QSAFTVPAYDLKSAAELPL
Sbjct: 301 FFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELPL 360

Query: 361 YLILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY 420
           YLILGMLCG VSVAVTRLVAWF KLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY
Sbjct: 361 YLILGMLCGVVSVAVTRLVAWFSKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY 420

Query: 421 WGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 480
           WGFTNVEEILHTGNRPSAPG+WLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA
Sbjct: 421 WGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 480

Query: 481 VFGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILL 540
           VFGGSAVE+IN AIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILL
Sbjct: 481 VFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILL 540

Query: 541 PLMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWR 600
           PLM           GAVGLAIWVPSVTKQTKEIESS+KR   RGY+SLSP   KDGA WR
Sbjct: 541 PLM-----------GAVGLAIWVPSVTKQTKEIESSEKRVSTRGYSSLSPPGHKDGASWR 600

Query: 601 YDNGDNALELSEVVNP----SGHEDNILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDN 660
           YD+GD  LELSEVV P    S +EDN+LE LKVS+ MSKNYLKV LS+YLKDALKYMKDN
Sbjct: 601 YDSGDKDLELSEVVTPSDRESNYEDNLLEKLKVSKAMSKNYLKVPLSLYLKDALKYMKDN 660

Query: 661 QQNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGR 720
           QQNCALVVD+DD LEGILT+GDIKRYLFKKYGD    DSLSVDTCLVSSIYTRGISYRGR
Sbjct: 661 QQNCALVVDNDDLLEGILTNGDIKRYLFKKYGDTLKSDSLSVDTCLVSSIYTRGISYRGR 720

Query: 721 ERGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCLREFVSQ 780
           ERGILTCYPD ALAIAKELMEAKG+KQLPV+KRG+ RKRRIVAVLHYDSIFSCLREF++Q
Sbjct: 721 ERGILTCYPDNALAIAKELMEAKGVKQLPVIKRGRGRKRRIVAVLHYDSIFSCLREFINQ 780

Query: 781 RETVYPSGKEIVAQETVADGH 797
           RE VYPSGKEIV QE+VAD H
Sbjct: 781 REKVYPSGKEIVVQESVADSH 790

BLAST of HG10003266 vs. ExPASy TrEMBL
Match: A0A6J1KYS2 (Chloride channel protein OS=Cucurbita maxima OX=3661 GN=LOC111498341 PE=3 SV=1)

HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 709/807 (87.86%), Postives = 735/807 (91.08%), Query Frame = 0

Query: 1   MAEGEFSDQNRLLRSMEDHNEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRGSSF 60
           MAE EFSDQNRLLRSMEDH  EDHDLESQDGNG+ P+RNNSGKRGG LD+ HHLNRG SF
Sbjct: 1   MAEAEFSDQNRLLRSMEDHKGEDHDLESQDGNGIQPLRNNSGKRGGILDMFHHLNRGGSF 60

Query: 61  SGRRLSYKRLDMDNHNVNFNPSSVNIVGRSRTA-SSSSSSDRHNNFNSPSFHAPTAIDGE 120
           SGRRLS KR DMDNH   F+P+S+NIVGR+RTA SSSSSSDRHNNFNS S HAPT  D E
Sbjct: 61  SGRRLSSKRHDMDNHAATFSPTSLNIVGRTRTASSSSSSSDRHNNFNSYSLHAPTVSDTE 120

Query: 121 IDDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQR 180
           IDDNVDDTA PEWALLLIACLLGLATGLCVAAFN+GVHVIHEWAWAGTPNEGAAWLRLQR
Sbjct: 121 IDDNVDDTAAPEWALLLIACLLGLATGLCVAAFNMGVHVIHEWAWAGTPNEGAAWLRLQR 180

Query: 181 MADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAI 240
           MADTWHRILLIPVTGGVIVGMMHGLLEIL QIKQSS SQ QGFDLLSGVFPTVKAIQAAI
Sbjct: 181 MADTWHRILLIPVTGGVIVGMMHGLLEILDQIKQSSTSQRQGFDLLSGVFPTVKAIQAAI 240

Query: 241 TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS 300
           TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS
Sbjct: 241 TLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGS 300

Query: 301 FFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPL 360
           FFAIETVLRPLRAENSPPFTTAMIIL+SVISSTVS VLLG QSAFTVPAYDLKSAAELPL
Sbjct: 301 FFAIETVLRPLRAENSPPFTTAMIILSSVISSTVSTVLLGTQSAFTVPAYDLKSAAELPL 360

Query: 361 YLILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY 420
           YLILGMLCG VSVAVTRLVAWF KLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY
Sbjct: 361 YLILGMLCGVVSVAVTRLVAWFSKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILY 420

Query: 421 WGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 480
           WGFTNVEEILHTGNRPSAPG+WLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA
Sbjct: 421 WGFTNVEEILHTGNRPSAPGVWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 480

Query: 481 VFGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILL 540
           VFGGSAVE+IN AIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILL
Sbjct: 481 VFGGSAVEVINAAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILL 540

Query: 541 PLMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWR 600
           PLM           GAVGLAIWVPSVTKQTKEIESS+KR   RGY+SLSP   KDGA WR
Sbjct: 541 PLM-----------GAVGLAIWVPSVTKQTKEIESSEKRVSTRGYSSLSPPGHKDGASWR 600

Query: 601 YDNGDNALELSEVVNP----SGHEDNILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDN 660
           YD+GD  LELSEVV P    S +EDN+LE LKVS+ MSKNYLKV LS+YLKDALKYMKDN
Sbjct: 601 YDSGDKDLELSEVVTPSDRESNYEDNLLEKLKVSKAMSKNYLKVPLSLYLKDALKYMKDN 660

Query: 661 QQNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGR 720
           QQNCALVVD+DD LEGILT+GDIKRYLFKKYGD    DSLSVDTCLVSSIYTRGISYRGR
Sbjct: 661 QQNCALVVDNDDLLEGILTNGDIKRYLFKKYGDTLKSDSLSVDTCLVSSIYTRGISYRGR 720

Query: 721 ERGILTCYPDTALAIAKELMEAKGIKQLPVVKRGKERKRRIVAVLHYDSIFSCL------ 780
           ERGILTCYPD ALAIAKELMEAKG+KQLPV+KRG+ RKRRIVAVLHYDSIFSCL      
Sbjct: 721 ERGILTCYPDNALAIAKELMEAKGVKQLPVIKRGRGRKRRIVAVLHYDSIFSCLRFLLNR 780

Query: 781 REFVSQRETVYPSGKEIVAQETVADGH 797
           REF++QRE VYPSGKEIV QE+VAD H
Sbjct: 781 REFINQREKVYPSGKEIVVQESVADSH 796

BLAST of HG10003266 vs. TAIR 10
Match: AT1G55620.2 (chloride channel F )

HSP 1 Score: 966.5 bits (2497), Expect = 1.4e-281
Identity = 537/810 (66.30%), Postives = 624/810 (77.04%), Query Frame = 0

Query: 4   GEFSDQNRLLRSMEDH------NEEDHDLESQDGNGVHPMRNNSGKRGGFLDLLHHLNRG 63
           GE+++   LLRS +         E D D+ESQ       +R+ +   GG  DL  H++R 
Sbjct: 7   GEYNEDRHLLRSTDGDEVGIGGGEGDLDVESQS----PAIRSGA---GGVRDLFKHIDRR 66

Query: 64  SSFSGRRLSYKRLDMDNHNVN---FNPSSVNIVGRSRTASSSSSSDRHNNFNSPSFHAPT 123
            S SGRRLS+KR  M+N  V+    NPSS      S   S++   D     N  S     
Sbjct: 67  FSLSGRRLSFKR--MENIRVDRERHNPSS------SSAFSAAGEEDGGGISNLHSVDDRN 126

Query: 124 AIDGEIDDNVDDTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAW 183
              G  ++ + D+APPEWALLLI CL+G+A G+CVA FN GVHVIHEWAWAGTPNEGAAW
Sbjct: 127 DEYGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAW 186

Query: 184 LRLQRMADTWHRILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKA 243
           LRLQR+ADTWHRILLIPVTGGVIVGMMHGLLEIL QI+QS++SQ QG D L+G++P +KA
Sbjct: 187 LRLQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGLDFLAGIYPVIKA 246

Query: 244 IQAAITLGTGCSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNA 303
           IQAA+TLGTGCSLGPEGPSVDIGKSCANGF LMMENN E+ +IA  AAGAA+GIASGFNA
Sbjct: 247 IQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRER-RIALTAAGAASGIASGFNA 306

Query: 304 AVAGSFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSA 363
           AVAG FFAIETVLRPLRAENSPPFTTAMIILASVISSTVSN LLG QSAFTVP+YDLKSA
Sbjct: 307 AVAGCFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNALLGTQSAFTVPSYDLKSA 366

Query: 364 AELPLYLILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKY 423
           AELPLYLILGMLCGAVSV  +RLV WF K F+FIK++FGLP +VCPALGGLGAGIIALKY
Sbjct: 367 AELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALKY 426

Query: 424 PGILYWGFTNVEEILHTGNRPSAPGIWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIG 483
           PGILYWGFTNVEEILHTG   SAPGIWLL Q+AAAKVVATALCKGSGLVGGLYAPSLMIG
Sbjct: 427 PGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMIG 486

Query: 484 AAVGAVFGGSAVEIINIAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKD 543
           AAVGAVFGGSA EIIN AIPGNAAVAQPQAYALVGMAATLAS+CSVPLTSVLLLFELTKD
Sbjct: 487 AAVGAVFGGSAAEIINRAIPGNAAVAQPQAYALVGMAATLASMCSVPLTSVLLLFELTKD 546

Query: 544 YRILLPLMAIEKLDTTFPWGAVGLAIWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKD 603
           YRILLPLM           GAVGLAIWVPSV  Q KE +SS+ R   RGY+SLSPSERK 
Sbjct: 547 YRILLPLM-----------GAVGLAIWVPSVANQGKESDSSEGRSTGRGYSSLSPSERKT 606

Query: 604 GAPWRYDNGDNALELSEVVNPSGH----EDNILEDLKVSQVMSKNYLKVSLSMYLKDALK 663
              WR+ +  ++LEL+ + NP  +    E+ ILEDLKV +VMSKNY+KVS    L++A  
Sbjct: 607 EGVWRHTDNADSLELTVIENPDHNSFLDEETILEDLKVMRVMSKNYVKVSSGTTLREARN 666

Query: 664 YMKDNQQNCALVVDDDDFLEGILTHGDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGI 723
            +K++ QNC +VVDDDDFL GILTHGDI+RYL            L  +TC VSS+ T+ I
Sbjct: 667 ILKESHQNCIMVVDDDDFLAGILTHGDIRRYLSNN-----ASTILDENTCPVSSVCTKKI 726

Query: 724 SYRGRERGILTCYPDTALAIAKELMEAKGIKQLPVVKRG----KERKRRIVAVLHYDSIF 783
           SYRG+ERG+LTCYPD  + +AKELMEA+G+KQLPVVKRG    K ++R+++ +LHYDSI+
Sbjct: 727 SYRGQERGLLTCYPDATVGVAKELMEARGVKQLPVVKRGEVIHKGKRRKLLGLLHYDSIW 781

Query: 784 SCLREFVSQRETVYPSGKEIVAQETVADGH 797
           + LR+ +S+R ++    K+   +E   +GH
Sbjct: 787 TFLRDEMSRRRSINDRRKD---KEVGTNGH 781

BLAST of HG10003266 vs. TAIR 10
Match: AT1G55620.1 (chloride channel F )

HSP 1 Score: 804.7 bits (2077), Expect = 6.8e-233
Identity = 431/605 (71.24%), Postives = 495/605 (81.82%), Query Frame = 0

Query: 200 MMHGLLEILSQIKQSSASQGQGFDLLSGVFPTVKAIQAAITLGTGCSLGPEGPSVDIGKS 259
           MMHGLLEIL QI+QS++SQ QG D L+G++P +KAIQAA+TLGTGCSLGPEGPSVDIGKS
Sbjct: 1   MMHGLLEILDQIRQSNSSQRQGLDFLAGIYPVIKAIQAAVTLGTGCSLGPEGPSVDIGKS 60

Query: 260 CANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIETVLRPLRAENSPPFT 319
           CANGF LMMENN E+ +IA  AAGAA+GIASGFNAAVAG FFAIETVLRPLRAENSPPFT
Sbjct: 61  CANGFALMMENNRER-RIALTAAGAASGIASGFNAAVAGCFFAIETVLRPLRAENSPPFT 120

Query: 320 TAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLYLILGMLCGAVSVAVTRLVA 379
           TAMIILASVISSTVSN LLG QSAFTVP+YDLKSAAELPLYLILGMLCGAVSV  +RLV 
Sbjct: 121 TAMIILASVISSTVSNALLGTQSAFTVPSYDLKSAAELPLYLILGMLCGAVSVVFSRLVT 180

Query: 380 WFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWGFTNVEEILHTGNRPSAPG 439
           WF K F+FIK++FGLP +VCPALGGLGAGIIALKYPGILYWGFTNVEEILHTG   SAPG
Sbjct: 181 WFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALKYPGILYWGFTNVEEILHTGKSASAPG 240

Query: 440 IWLLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSAVEIINIAIPGNAAV 499
           IWLL Q+AAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSA EIIN AIPGNAAV
Sbjct: 241 IWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFGGSAAEIINRAIPGNAAV 300

Query: 500 AQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLPLMAIEKLDTTFPWGAVGLA 559
           AQPQAYALVGMAATLAS+CSVPLTSVLLLFELTKDYRILLPLM           GAVGLA
Sbjct: 301 AQPQAYALVGMAATLASMCSVPLTSVLLLFELTKDYRILLPLM-----------GAVGLA 360

Query: 560 IWVPSVTKQTKEIESSDKRGLARGYTSLSPSERKDGAPWRYDNGDNALELSEVVNPSGH- 619
           IWVPSV  Q KE +SS+ R   RGY+SLSPSERK    WR+ +  ++LEL+ + NP  + 
Sbjct: 361 IWVPSVANQGKESDSSEGRSTGRGYSSLSPSERKTEGVWRHTDNADSLELTVIENPDHNS 420

Query: 620 ---EDNILEDLKVSQVMSKNYLKVSLSMYLKDALKYMKDNQQNCALVVDDDDFLEGILTH 679
              E+ ILEDLKV +VMSKNY+KVS    L++A   +K++ QNC +VVDDDDFL GILTH
Sbjct: 421 FLDEETILEDLKVMRVMSKNYVKVSSGTTLREARNILKESHQNCIMVVDDDDFLAGILTH 480

Query: 680 GDIKRYLFKKYGDAFMGDSLSVDTCLVSSIYTRGISYRGRERGILTCYPDTALAIAKELM 739
           GDI+RYL            L  +TC VSS+ T+ ISYRG+ERG+LTCYPD  + +AKELM
Sbjct: 481 GDIRRYLSNN-----ASTILDENTCPVSSVCTKKISYRGQERGLLTCYPDATVGVAKELM 540

Query: 740 EAKGIKQLPVVKRG----KERKRRIVAVLHYDSIFSCLREFVSQRETVYPSGKEIVAQET 797
           EA+G+KQLPVVKRG    K ++R+++ +LHYDSI++ LR+ +S+R ++    K+   +E 
Sbjct: 541 EARGVKQLPVVKRGEVIHKGKRRKLLGLLHYDSIWTFLRDEMSRRRSINDRRKD---KEV 585

BLAST of HG10003266 vs. TAIR 10
Match: AT4G35440.1 (chloride channel E )

HSP 1 Score: 397.9 bits (1021), Expect = 1.9e-110
Identity = 258/638 (40.44%), Postives = 370/638 (57.99%), Query Frame = 0

Query: 126 DTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMADTWH 185
           D  P +   +  ACL+G+ TG+ V  FN  VH++ +++W G P+ GA+WLR   +   W 
Sbjct: 68  DQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRGASWLREAPIGSNWL 127

Query: 186 RILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVF-PTVKAIQAAITLGTG 245
           R++L+P  GG++V +++ L E      +S+       D +  V  P +K + A +TLGTG
Sbjct: 128 RVILVPTIGGLVVSILNQLRE---SAGKSTGDSHSSLDRVKAVLRPFLKTVAACVTLGTG 187

Query: 246 CSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIE 305
            SLGPEGPSV+IG S A G   +  N S +   + +AAG+AAGI+SGFNAAVAG FFA+E
Sbjct: 188 NSLGPEGPSVEIGASIAKGVNSLF-NKSPQTGFSLLAAGSAAGISSGFNAAVAGCFFAVE 247

Query: 306 TVLRPLRAENSP---PFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLYL 365
           +VL P  + +S    P TT+M+IL++V +S VS + LG++ AF VP YD +S  ELPLYL
Sbjct: 248 SVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFRSPGELPLYL 307

Query: 366 ILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 425
           +LG LCG VS+A++R  +      + + +  G+P  V P +GGL  GIIAL YP +LYWG
Sbjct: 308 LLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIALVYPEVLYWG 367

Query: 426 FTNVEEILHTGNRPSAPGIW--LLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 485
           F NV+ +L    RP   G+   LL Q+ A K+ ATA C+ SGLVGG YAPSL IG A G 
Sbjct: 368 FQNVDILLE--KRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLFIGGAAGM 427

Query: 486 VFGGSAVEIINIAIPGN-------AAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELT 545
            +G    + I +A+  N         VA PQAY LVGMAATLA VC VPLT+VLLLFELT
Sbjct: 428 AYG----KFIGLALAQNPDFNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELT 487

Query: 546 KDYRILLPLMAIEKLDTTFPWGAVGLAIWV---PSVTKQTKEIESSDKRGLARGYTSLSP 605
           +DYRI+LPL+           GAVG++ W+    S  ++T+E + + KR       SL+ 
Sbjct: 488 QDYRIVLPLL-----------GAVGMSSWITSGQSKRQETRETKETRKRKSQEAVQSLTS 547

Query: 606 SERKDGAPWRYDNGDNALELSEVVNPSGHEDNILEDLKVSQVMSKNYLKVSLSMYLKDAL 665
           S+  + +       +++L L + +N S   + + + + VS+ M   +  V +S  L++AL
Sbjct: 548 SD-DESSTNNLCEVESSLCLDDSLNQS---EELPKSIFVSEAMRTRFATVMMSTSLEEAL 607

Query: 666 KYMKDNQQNCALVVDDDDFLEGILTHGDIKRY-LFKKYGDAFMGDSLSVDTCLVSSIYTR 725
             M   +Q+CAL+VD D+   GILT  DI+ +   +K G+    D    D C  S     
Sbjct: 608 TRMLIEKQSCALIVDPDNIFLGILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSG---- 667

Query: 726 GISYRGRERGILTCYPDTALAIAKELMEAKGIKQLPVV 747
                G+ +   T  PD  L  A+ +M    +  + VV
Sbjct: 668 -----GKCKVPWTVTPDMDLLAAQTIMNKHELSHVAVV 671

BLAST of HG10003266 vs. TAIR 10
Match: AT4G35440.2 (chloride channel E )

HSP 1 Score: 397.9 bits (1021), Expect = 1.9e-110
Identity = 258/638 (40.44%), Postives = 370/638 (57.99%), Query Frame = 0

Query: 126 DTAPPEWALLLIACLLGLATGLCVAAFNIGVHVIHEWAWAGTPNEGAAWLRLQRMADTWH 185
           D  P +   +  ACL+G+ TG+ V  FN  VH++ +++W G P+ GA+WLR   +   W 
Sbjct: 68  DQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRGASWLREAPIGSNWL 127

Query: 186 RILLIPVTGGVIVGMMHGLLEILSQIKQSSASQGQGFDLLSGVF-PTVKAIQAAITLGTG 245
           R++L+P  GG++V +++ L E      +S+       D +  V  P +K + A +TLGTG
Sbjct: 128 RVILVPTIGGLVVSILNQLRE---SAGKSTGDSHSSLDRVKAVLRPFLKTVAACVTLGTG 187

Query: 246 CSLGPEGPSVDIGKSCANGFYLMMENNSEKIKIAFVAAGAAAGIASGFNAAVAGSFFAIE 305
            SLGPEGPSV+IG S A G   +  N S +   + +AAG+AAGI+SGFNAAVAG FFA+E
Sbjct: 188 NSLGPEGPSVEIGASIAKGVNSLF-NKSPQTGFSLLAAGSAAGISSGFNAAVAGCFFAVE 247

Query: 306 TVLRPLRAENSP---PFTTAMIILASVISSTVSNVLLGAQSAFTVPAYDLKSAAELPLYL 365
           +VL P  + +S    P TT+M+IL++V +S VS + LG++ AF VP YD +S  ELPLYL
Sbjct: 248 SVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFRSPGELPLYL 307

Query: 366 ILGMLCGAVSVAVTRLVAWFGKLFEFIKERFGLPPVVCPALGGLGAGIIALKYPGILYWG 425
           +LG LCG VS+A++R  +      + + +  G+P  V P +GGL  GIIAL YP +LYWG
Sbjct: 308 LLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIALVYPEVLYWG 367

Query: 426 FTNVEEILHTGNRPSAPGIW--LLTQIAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGA 485
           F NV+ +L    RP   G+   LL Q+ A K+ ATA C+ SGLVGG YAPSL IG A G 
Sbjct: 368 FQNVDILLE--KRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLFIGGAAGM 427

Query: 486 VFGGSAVEIINIAIPGN-------AAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELT 545
            +G    + I +A+  N         VA PQAY LVGMAATLA VC VPLT+VLLLFELT
Sbjct: 428 AYG----KFIGLALAQNPDFNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELT 487

Query: 546 KDYRILLPLMAIEKLDTTFPWGAVGLAIWV---PSVTKQTKEIESSDKRGLARGYTSLSP 605
           +DYRI+LPL+           GAVG++ W+    S  ++T+E + + KR       SL+ 
Sbjct: 488 QDYRIVLPLL-----------GAVGMSSWITSGQSKRQETRETKETRKRKSQEAVQSLTS 547

Query: 606 SERKDGAPWRYDNGDNALELSEVVNPSGHEDNILEDLKVSQVMSKNYLKVSLSMYLKDAL 665
           S+  + +       +++L L + +N S   + + + + VS+ M   +  V +S  L++AL
Sbjct: 548 SD-DESSTNNLCEVESSLCLDDSLNQS---EELPKSIFVSEAMRTRFATVMMSTSLEEAL 607

Query: 666 KYMKDNQQNCALVVDDDDFLEGILTHGDIKRY-LFKKYGDAFMGDSLSVDTCLVSSIYTR 725
             M   +Q+CAL+VD D+   GILT  DI+ +   +K G+    D    D C  S     
Sbjct: 608 TRMLIEKQSCALIVDPDNIFLGILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSG---- 667

Query: 726 GISYRGRERGILTCYPDTALAIAKELMEAKGIKQLPVV 747
                G+ +   T  PD  L  A+ +M    +  + VV
Sbjct: 668 -----GKCKVPWTVTPDMDLLAAQTIMNKHELSHVAVV 671