HG10003254 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003254
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description(-)-germacrene D synthase-like
LocationChr11: 19355128 .. 19355787 (+)
RNA-Seq ExpressionHG10003254
SyntenyHG10003254
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAAGACAATGCAGAGCTTATTTTATGGAAGCTAAATGGTTCCGTGAAGGTTATATACCAACAATGGAAGAATATATGAAAGTTGCCATCATTTCTGCTTCCTACTATCTTTTTGCTTCAGCTTCCTTCCTTTCTTTAAGAAATGTTGCTTCAAAAGAGGTTTTTGAATGGGCTCAAACTGACCCTACGCTTCTTAGAGCAACTGGAATAATTGGGAGGCTCCTCAATGACATAGTTTCCCACAAGGTACACAAAGAAAAAAATGATCAATTTGTAATATATAGCAAATGATGCAATCTTTTAATTTGATAACGTGTTCTTGTTCTAAATTTATTATATGTTTATTCGTATTATTTCTACTAGTTTGAACAAGAGAGAGGTCATGTTGCTTCTGCGGTAGAATGTTACATGAAGGAATATGGAGTATCAGAAGAAGAGGCCCTAGTTGAACTAAAAAATCAAGTGGAAACAGCATGGAAAGATGTTACAGAAGACTACACAAAATCTTCCAAGTTTCCAAATGTGATCCTTGATTGTGTGTTGAATGTTGCTCGACTTTCAAACCTCTTTTATAAGGAGGAAGATGGTTATACATTCGTTAATGGAGAAACCAAACATTTTATTACCTTAATGCTTAAGGACCCTGTGCCAATTTGA

mRNA sequence

ATGAAAAGACAATGCAGAGCTTATTTTATGGAAGCTAAATGGTTCCGTGAAGGTTATATACCAACAATGGAAGAATATATGAAAGTTGCCATCATTTCTGCTTCCTACTATCTTTTTGCTTCAGCTTCCTTCCTTTCTTTAAGAAATGTTGCTTCAAAAGAGGTTTTTGAATGGGCTCAAACTGACCCTACGCTTCTTAGAGCAACTGGAATAATTGGGAGGCTCCTCAATGACATAGTTTCCCACAAGTTTGAACAAGAGAGAGGTCATGTTGCTTCTGCGGTAGAATGTTACATGAAGGAATATGGAGTATCAGAAGAAGAGGCCCTAGTTGAACTAAAAAATCAAGTGGAAACAGCATGGAAAGATGTTACAGAAGACTACACAAAATCTTCCAAGTTTCCAAATGTGATCCTTGATTGTGTGTTGAATGTTGCTCGACTTTCAAACCTCTTTTATAAGGAGGAAGATGGTTATACATTCGTTAATGGAGAAACCAAACATTTTATTACCTTAATGCTTAAGGACCCTGTGCCAATTTGA

Coding sequence (CDS)

ATGAAAAGACAATGCAGAGCTTATTTTATGGAAGCTAAATGGTTCCGTGAAGGTTATATACCAACAATGGAAGAATATATGAAAGTTGCCATCATTTCTGCTTCCTACTATCTTTTTGCTTCAGCTTCCTTCCTTTCTTTAAGAAATGTTGCTTCAAAAGAGGTTTTTGAATGGGCTCAAACTGACCCTACGCTTCTTAGAGCAACTGGAATAATTGGGAGGCTCCTCAATGACATAGTTTCCCACAAGTTTGAACAAGAGAGAGGTCATGTTGCTTCTGCGGTAGAATGTTACATGAAGGAATATGGAGTATCAGAAGAAGAGGCCCTAGTTGAACTAAAAAATCAAGTGGAAACAGCATGGAAAGATGTTACAGAAGACTACACAAAATCTTCCAAGTTTCCAAATGTGATCCTTGATTGTGTGTTGAATGTTGCTCGACTTTCAAACCTCTTTTATAAGGAGGAAGATGGTTATACATTCGTTAATGGAGAAACCAAACATTTTATTACCTTAATGCTTAAGGACCCTGTGCCAATTTGA

Protein sequence

MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQTDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETAWKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI
Homology
BLAST of HG10003254 vs. NCBI nr
Match: XP_038903578.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 313.2 bits (801), Expect = 1.5e-81
Identity = 150/180 (83.33%), Postives = 165/180 (91.67%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MKRQCRAYFMEAKWF EGYIPTMEEYMKVA+ISA+YYLFAS SFL+L N+AS+EVFEWAQ
Sbjct: 315 MKRQCRAYFMEAKWFNEGYIPTMEEYMKVALISAAYYLFASVSFLALGNIASREVFEWAQ 374

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           TDP LL+ATG+IGRLLNDI SHKFEQ+RGHVASA+ECYMKEYGVSEEEAL +L  +VE A
Sbjct: 375 TDPMLLKATGVIGRLLNDIASHKFEQKRGHVASAIECYMKEYGVSEEEALTKLDKEVERA 434

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 180
           WKDVT+DY KSSKFPNVILDCVLNVARLS+LFYKE DGYTF +GETK FITL+L  PVPI
Sbjct: 435 WKDVTKDYIKSSKFPNVILDCVLNVARLSDLFYKEGDGYTFADGETKQFITLLLIHPVPI 494

BLAST of HG10003254 vs. NCBI nr
Match: XP_016900585.1 (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 290.8 bits (743), Expect = 8.0e-75
Identity = 143/179 (79.89%), Postives = 159/179 (88.83%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           +K  C+AYFMEAKWF EGYIPT+EEYMKVA ISASY +FAS SFL+L NVASKE +EWAQ
Sbjct: 384 LKSLCKAYFMEAKWFNEGYIPTVEEYMKVARISASYNVFASISFLTLGNVASKEFYEWAQ 443

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           TDP LL+A G+IGRLLND+VSHKFEQERGHVASAVECY+KEY VSEEEAL+EL+ +V TA
Sbjct: 444 TDPILLKAAGVIGRLLNDMVSHKFEQERGHVASAVECYVKEYEVSEEEALIELEKEVGTA 503

Query: 121 WKDVTEDYTKSS-KFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPV 179
           WKDVTEDYTKSS KFPNVIL+CVLNVARLS+ FYKE DGYTF +GETKHFI LML  PV
Sbjct: 504 WKDVTEDYTKSSLKFPNVILECVLNVARLSDFFYKEVDGYTFADGETKHFIDLMLTHPV 562

BLAST of HG10003254 vs. NCBI nr
Match: XP_038877743.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 290.0 bits (741), Expect = 1.4e-74
Identity = 139/181 (76.80%), Postives = 160/181 (88.40%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MK QCRAY MEAKWF + YIPTMEEYMKVAIISA+YY+ AS SF++L NVASKE+FEWAQ
Sbjct: 382 MKMQCRAYLMEAKWFNQRYIPTMEEYMKVAIISAAYYVVASISFIALGNVASKEIFEWAQ 441

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           TDP L++ATG+IGRLLNDI SHKFEQERGHVASA+ECYMK+YGVSEEEA+VELK QV  A
Sbjct: 442 TDPMLIKATGVIGRLLNDIASHKFEQERGHVASAIECYMKQYGVSEEEAVVELKLQVVKA 501

Query: 121 WKDVTEDYTKSSK-FPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVP 180
           WKDV ED+T+SS+ FP VI DC+LN+ARL++LFYK+ DGYTF +GE KHFIT ML DPVP
Sbjct: 502 WKDVIEDHTESSQLFPTVICDCILNIARLTDLFYKDGDGYTFADGEIKHFITFMLIDPVP 561

BLAST of HG10003254 vs. NCBI nr
Match: XP_031738278.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase [Cucumis sativus])

HSP 1 Score: 288.9 bits (738), Expect = 3.0e-74
Identity = 141/180 (78.33%), Postives = 158/180 (87.78%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MK  C+AYFMEAKWF EGY+P +EEYMKVA ISASY +FAS SFLSL NVASKEV+EWAQ
Sbjct: 391 MKSLCKAYFMEAKWFNEGYVPKVEEYMKVASISASYNVFASISFLSLGNVASKEVYEWAQ 450

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           T P LL+ATG++GRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEA++EL+ +V TA
Sbjct: 451 TQPILLKATGVMGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEAIIELEKEVGTA 510

Query: 121 WKDVTEDYTK-SSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVP 180
           WKDVTEDYTK S K PNVIL+CVLNVAR+S+ FYK+ DGYTF + ETKHFI LML  PVP
Sbjct: 511 WKDVTEDYTKFSLKLPNVILECVLNVARISDFFYKKVDGYTFADEETKHFIDLMLTHPVP 570

BLAST of HG10003254 vs. NCBI nr
Match: KGN55986.2 (hypothetical protein Csa_011696 [Cucumis sativus])

HSP 1 Score: 286.6 bits (732), Expect = 1.5e-73
Identity = 140/182 (76.92%), Postives = 160/182 (87.91%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSL-RNVASKEVFEWA 60
           MK QCRA+FMEAKWF EGYIPT+EEYMKV+I SA+YY+FAS SFL++  N ASK+V+EWA
Sbjct: 416 MKNQCRAFFMEAKWFNEGYIPTVEEYMKVSITSAAYYVFASISFLTIGNNAASKQVYEWA 475

Query: 61  QTDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVET 120
           QTDP LLRAT +IGR +NDI SHKFEQERGHVASAVECYMK+YGVSEEEA+ EL+ QV  
Sbjct: 476 QTDPILLRATSVIGRFVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVTELQKQVVN 535

Query: 121 AWKDVTEDYTKSSK-FPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPV 180
           AWKD+ EDY KSSK FP+ ILD VLNVARLS+LFYKEEDGYTF +GETK FITLMLK+P+
Sbjct: 536 AWKDIIEDYMKSSKLFPSFILDHVLNVARLSDLFYKEEDGYTFADGETKRFITLMLKNPM 595

BLAST of HG10003254 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.6e-46
Identity = 90/180 (50.00%), Postives = 124/180 (68.89%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MK Q RAY+ EAKW +   IPTMEEYM VA+++++Y + A+ SF+ + +  +KE F+W  
Sbjct: 378 MKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATTSFVGMGDAVTKESFDWIF 437

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           + P ++RA+ I+ RL++D+V HKFEQ+RGHVASAVECYMK++G SE+E   E    V  A
Sbjct: 438 SKPKIVRASAIVCRLMDDMVFHKFEQKRGHVASAVECYMKQHGASEQETPNEFPQPVREA 497

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 180
           WKD+ E+    +  P  IL  VLN+AR+ ++ YK EDGYT      K F+T ML DPVPI
Sbjct: 498 WKDINEECLIPTAVPMPILMRVLNLARVIDVIYKNEDGYTHFGAVLKDFVTSMLIDPVPI 557

BLAST of HG10003254 vs. ExPASy Swiss-Prot
Match: B9S9Z3 (Alpha-copaene synthase OS=Ricinus communis OX=3988 GN=TPS1 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.8e-43
Identity = 84/180 (46.67%), Postives = 122/180 (67.78%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MKR  RAY  E +W    Y PTMEEYM+ A+++    + A ASFL L+ +A+KE +EWA 
Sbjct: 380 MKRVVRAYLAEVRWRDNCYTPTMEEYMQSALLTTCSPMLAIASFLGLKEIATKEAYEWAS 439

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
            DP ++RA+ I+ RL++DIVSH+FEQ R HVAS VECY+K+YG SEEE +   + +V  A
Sbjct: 440 EDPKIIRASSIVCRLMDDIVSHEFEQTRKHVASGVECYIKQYGASEEEVIKLFRKEVTNA 499

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 180
           WKD+ E+    +  P  +L+ V+N+ R  ++ YK++DGYT  +   K ++  +LKDPVP+
Sbjct: 500 WKDLNEECLNPTPVPMPMLERVVNLTRAIDVIYKDDDGYT-NSHIMKDYVASVLKDPVPV 558

BLAST of HG10003254 vs. ExPASy Swiss-Prot
Match: F6M8H7 (Probable sesquiterpene synthase OS=Santalum murrayanum OX=453085 GN=STPS PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 6.4e-43
Identity = 81/178 (45.51%), Postives = 117/178 (65.73%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MK     Y  EAKW  E Y+PT +EYM VA++++ Y    + S+L +  +ASKE F+W  
Sbjct: 383 MKTIVEGYMDEAKWCHEKYVPTFQEYMSVALVTSGYTFLTTISYLGMGEIASKEAFDWLF 442

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           + P ++ A+  +GRL++D+ SHKFEQERGHVAS +ECYMK+YGV+EEEA  E + ++  A
Sbjct: 443 SHPPVIEASESVGRLMDDMRSHKFEQERGHVASGIECYMKQYGVTEEEAHDEFRKRLVKA 502

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPV 179
           WKD+ E+  +  + P  +L  +LN+ R+ ++ YK EDGYT V    K  I  +L DPV
Sbjct: 503 WKDINEECLRPYRVPKPLLTRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLIDPV 560

BLAST of HG10003254 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 6.4e-43
Identity = 80/177 (45.20%), Postives = 118/177 (66.67%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MK+  +AY +EA W  + Y+PTM+EYM +A++S +Y L    SF+ + ++A+KEVF+WA 
Sbjct: 373 MKKLVQAYLVEANWMNKNYVPTMDEYMSIALVSCAYPLLTVTSFVGMGDIATKEVFDWAS 432

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
            DP ++R   II RL++DIVSH+FEQ+RGH+AS+VECYMK+ GVSEE    E   Q+  A
Sbjct: 433 NDPKIVRVASIICRLMDDIVSHEFEQKRGHIASSVECYMKQNGVSEEATRDEFNKQIVDA 492

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDP 178
           WKD+ E++ + +  P      V+N AR+ +  YK++D YT V    K  +  +L DP
Sbjct: 493 WKDINEEHLQPNYVPMPFRTRVVNSARIMDYLYKDDDEYTHVGELMKGSVAALLIDP 549

BLAST of HG10003254 vs. ExPASy Swiss-Prot
Match: F6M8H5 (Probable sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 GN=SesquiTPS PE=3 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.1e-42
Identity = 80/178 (44.94%), Postives = 117/178 (65.73%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MK+    Y  EAKW  E Y+PT +EYM VA++++ Y    + S+L +  +ASKE F+W  
Sbjct: 383 MKKLVEGYMDEAKWCHEKYVPTFQEYMSVALVTSGYTFLTTISYLGMGEIASKEAFDWLF 442

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           + P ++ A+  +GRL++D  SHKFEQERGHVAS +ECYMK+YGV+EEEA  E + ++  A
Sbjct: 443 SHPPVIEASESVGRLMDDTRSHKFEQERGHVASGIECYMKQYGVTEEEARDEFRKRLVKA 502

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPV 179
           WKD+ E+  +  + P  +L  +LN+ R+ ++ YK EDGYT V    K  I  +L DP+
Sbjct: 503 WKDINEECLRPYRVPKPLLMRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLIDPM 560

BLAST of HG10003254 vs. ExPASy TrEMBL
Match: A0A1S4DXZ8 ((-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103491132 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.9e-75
Identity = 143/179 (79.89%), Postives = 159/179 (88.83%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           +K  C+AYFMEAKWF EGYIPT+EEYMKVA ISASY +FAS SFL+L NVASKE +EWAQ
Sbjct: 384 LKSLCKAYFMEAKWFNEGYIPTVEEYMKVARISASYNVFASISFLTLGNVASKEFYEWAQ 443

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           TDP LL+A G+IGRLLND+VSHKFEQERGHVASAVECY+KEY VSEEEAL+EL+ +V TA
Sbjct: 444 TDPILLKAAGVIGRLLNDMVSHKFEQERGHVASAVECYVKEYEVSEEEALIELEKEVGTA 503

Query: 121 WKDVTEDYTKSS-KFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPV 179
           WKDVTEDYTKSS KFPNVIL+CVLNVARLS+ FYKE DGYTF +GETKHFI LML  PV
Sbjct: 504 WKDVTEDYTKSSLKFPNVILECVLNVARLSDFFYKEVDGYTFADGETKHFIDLMLTHPV 562

BLAST of HG10003254 vs. ExPASy TrEMBL
Match: A0A0A0L791 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G042380 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.5e-74
Identity = 141/180 (78.33%), Postives = 158/180 (87.78%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MK  C+AYFMEAKWF EGY+P +EEYMKVA ISASY +FAS SFLSL NVASKEV+EWAQ
Sbjct: 285 MKSLCKAYFMEAKWFNEGYVPKVEEYMKVASISASYNVFASISFLSLGNVASKEVYEWAQ 344

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           T P LL+ATG++GRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEA++EL+ +V TA
Sbjct: 345 TQPILLKATGVMGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEAIIELEKEVGTA 404

Query: 121 WKDVTEDYTK-SSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVP 180
           WKDVTEDYTK S K PNVIL+CVLNVAR+S+ FYK+ DGYTF + ETKHFI LML  PVP
Sbjct: 405 WKDVTEDYTKFSLKLPNVILECVLNVARISDFFYKKVDGYTFADEETKHFIDLMLTHPVP 464

BLAST of HG10003254 vs. ExPASy TrEMBL
Match: A0A0A0L5V3 (Terpene_synth_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G040860 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.3e-74
Identity = 140/182 (76.92%), Postives = 160/182 (87.91%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSL-RNVASKEVFEWA 60
           MK QCRA+FMEAKWF EGYIPT+EEYMKV+I SA+YY+FAS SFL++  N ASK+V+EWA
Sbjct: 1   MKNQCRAFFMEAKWFNEGYIPTVEEYMKVSITSAAYYVFASISFLTIGNNAASKQVYEWA 60

Query: 61  QTDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVET 120
           QTDP LLRAT +IGR +NDI SHKFEQERGHVASAVECYMK+YGVSEEEA+ EL+ QV  
Sbjct: 61  QTDPILLRATSVIGRFVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVTELQKQVVN 120

Query: 121 AWKDVTEDYTKSSK-FPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPV 180
           AWKD+ EDY KSSK FP+ ILD VLNVARLS+LFYKEEDGYTF +GETK FITLMLK+P+
Sbjct: 121 AWKDIIEDYMKSSKLFPSFILDHVLNVARLSDLFYKEEDGYTFADGETKRFITLMLKNPM 180

BLAST of HG10003254 vs. ExPASy TrEMBL
Match: A0A6J1CP21 (valencene synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013401 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.3e-74
Identity = 136/180 (75.56%), Postives = 156/180 (86.67%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MKRQCRAYF EAKWF EGY+PTMEEYM+VA ISASY+LFA+ASFL + +VAS EVF+WA+
Sbjct: 301 MKRQCRAYFTEAKWFYEGYVPTMEEYMEVASISASYHLFAAASFLGMGDVASNEVFQWAK 360

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           +DP LL ATG+IGRL+NDIVSHKFEQERGHVASAVECYMK+YGVSE EA+ EL+ QV  A
Sbjct: 361 SDPILLSATGVIGRLMNDIVSHKFEQERGHVASAVECYMKQYGVSEGEAIHELEKQVIKA 420

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 180
           WKD+ EDY K ++FPN IL CV+NV RLS+ FYKEEDGYTF NGET  FIT MLKDPVP+
Sbjct: 421 WKDIIEDYMKCTRFPNAILSCVMNVDRLSDFFYKEEDGYTFPNGETNRFITSMLKDPVPL 480

BLAST of HG10003254 vs. ExPASy TrEMBL
Match: A0A6J1CPK3 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013401 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.3e-74
Identity = 136/180 (75.56%), Postives = 156/180 (86.67%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           MKRQCRAYF EAKWF EGY+PTMEEYM+VA ISASY+LFA+ASFL + +VAS EVF+WA+
Sbjct: 374 MKRQCRAYFTEAKWFYEGYVPTMEEYMEVASISASYHLFAAASFLGMGDVASNEVFQWAK 433

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           +DP LL ATG+IGRL+NDIVSHKFEQERGHVASAVECYMK+YGVSE EA+ EL+ QV  A
Sbjct: 434 SDPILLSATGVIGRLMNDIVSHKFEQERGHVASAVECYMKQYGVSEGEAIHELEKQVIKA 493

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 180
           WKD+ EDY K ++FPN IL CV+NV RLS+ FYKEEDGYTF NGET  FIT MLKDPVP+
Sbjct: 494 WKDIIEDYMKCTRFPNAILSCVMNVDRLSDFFYKEEDGYTFPNGETNRFITSMLKDPVPL 553

BLAST of HG10003254 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 141.0 bits (354), Expect = 9.6e-34
Identity = 65/180 (36.11%), Postives = 115/180 (63.89%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           +++    Y  EAKW ++ YI T +EY + AI+S+ YY   + +F+ + +VA  + FEW  
Sbjct: 369 LQKTANGYMQEAKWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLS 428

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           + P +  A+ II R  +DI S++FE +R HVA+ ++CYM+++GVS+E A+  + N V  A
Sbjct: 429 SHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDA 488

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 180
           WKD+ ++  +   FP  +L  VLN++R+ ++FY+ +D YT      +H ++L++ + +PI
Sbjct: 489 WKDLNQELMRPHVFPFPLLMRVLNLSRVIDVFYRYQDAYTNPKLLKEHIVSLLI-ETIPI 547

BLAST of HG10003254 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 141.0 bits (354), Expect = 9.6e-34
Identity = 65/180 (36.11%), Postives = 115/180 (63.89%), Query Frame = 0

Query: 1   MKRQCRAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQ 60
           +++    Y  EAKW ++ YI T +EY + AI+S+ YY   + +F+ + +VA  + FEW  
Sbjct: 367 LQKTANGYMQEAKWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLS 426

Query: 61  TDPTLLRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETA 120
           + P +  A+ II R  +DI S++FE +R HVA+ ++CYM+++GVS+E A+  + N V  A
Sbjct: 427 SHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDA 486

Query: 121 WKDVTEDYTKSSKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 180
           WKD+ ++  +   FP  +L  VLN++R+ ++FY+ +D YT      +H ++L++ + +PI
Sbjct: 487 WKDLNQELMRPHVFPFPLLMRVLNLSRVIDVFYRYQDAYTNPKLLKEHIVSLLI-ETIPI 545

BLAST of HG10003254 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 135.2 bits (339), Expect = 5.2e-32
Identity = 65/176 (36.93%), Postives = 108/176 (61.36%), Query Frame = 0

Query: 6   RAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQTDPTL 65
           +AY    +W R+G++PT +EYMKV +++A    +A   F+ + ++  KE FEW  ++P L
Sbjct: 426 KAYKQLTEWARKGHVPTFDEYMKVGLVTAGMGDYAGYCFIGMEDINEKEAFEWLNSNPLL 485

Query: 66  LRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETAWKDVT 125
           + A  ++ R+ ND+ +++ E  RG VA+ + CYMK+YGV++EEA  EL+       K V 
Sbjct: 486 IDALNVLFRIANDVGTYETEINRGEVANGLNCYMKQYGVTKEEASRELRKMYIYNKKVVV 545

Query: 126 EDYTKS-SKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 181
           E++  S  + P  +L   LN ARL ++ Y E DGY+   G+ +HF+T +   P+P+
Sbjct: 546 EEFMNSHDRVPRQVLLRCLNFARLFDVIYTEGDGYSEPKGKIEHFMTSLYVHPIPL 601

BLAST of HG10003254 vs. TAIR 10
Match: AT1G31950.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 1.5e-31
Identity = 65/176 (36.93%), Postives = 108/176 (61.36%), Query Frame = 0

Query: 6   RAYFMEAKWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQTDPTL 65
           +AY   AKW R G++ T +EYMKV +++A    +A+  F+ + ++  KE FEW  ++P +
Sbjct: 430 KAYQEIAKWARTGHVSTFDEYMKVGVLTAGMADYAAYCFIGMEDINEKEAFEWLNSNPLI 489

Query: 66  LRATGIIGRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETAWKDVT 125
           ++    + RL ND+ +++ E  RG VA+ + CYMK+YGV++EEA  EL+       K V 
Sbjct: 490 IKHLTAMFRLANDVGTYETEINRGEVANGLNCYMKQYGVTKEEASRELRKMYVYRKKVVV 549

Query: 126 EDYTKS-SKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 181
           E++  S    P  +L   LN+AR+ ++FY E DGY+   G+ +HF+T +   P+P+
Sbjct: 550 EEFMNSHDHVPRQVLLRCLNIARIFDVFYTEGDGYSEPKGKIEHFMTSLYLHPIPL 605

BLAST of HG10003254 vs. TAIR 10
Match: AT3G14520.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 1.7e-30
Identity = 63/169 (37.28%), Postives = 104/169 (61.54%), Query Frame = 0

Query: 13  KWFREGYIPTMEEYMKVAIISASYYLFASASFLSLRNVASKEVFEWAQTDPTLLRATGII 72
           +W R G +P+ +EYMKV +++A    +A   F+ + +V+ KE FEW  ++P +++A  ++
Sbjct: 436 EWARTGDVPSFDEYMKVGLVTAGMDGYAGYCFIGMEDVSEKEAFEWLSSNPLIIQALNVM 495

Query: 73  GRLLNDIVSHKFEQERGHVASAVECYMKEYGVSEEEALVELKNQVETAWKDVTEDYTKS- 132
            RL ND+ +++ E  RG VA+ + CYMK+YGV++EEA  EL+       K V E++  S 
Sbjct: 496 FRLANDVGTYETEINRGEVANGLNCYMKQYGVTKEEASQELRKIYSNNKKVVMEEFMNSH 555

Query: 133 SKFPNVILDCVLNVARLSNLFYKEEDGYTFVNGETKHFITLMLKDPVPI 181
              P  +L   LN ARL ++ Y E DGY+   G+ +HF+T +   P+P+
Sbjct: 556 DHVPRQVLLRCLNFARLFDVMYTEGDGYSEPKGKIEHFMTSLYVHPIPL 604

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903578.11.5e-8183.33LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida][more]
XP_016900585.18.0e-7579.89PREDICTED: (-)-germacrene D synthase-like [Cucumis melo][more]
XP_038877743.11.4e-7476.80LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida][more]
XP_031738278.13.0e-7478.33LOW QUALITY PROTEIN: (-)-germacrene D synthase [Cucumis sativus][more]
KGN55986.21.5e-7376.92hypothetical protein Csa_011696 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q6Q3H33.6e-4650.00(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S... [more]
B9S9Z33.8e-4346.67Alpha-copaene synthase OS=Ricinus communis OX=3988 GN=TPS1 PE=1 SV=1[more]
F6M8H76.4e-4345.51Probable sesquiterpene synthase OS=Santalum murrayanum OX=453085 GN=STPS PE=3 SV... [more]
B9SCB66.4e-4345.20Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1[more]
F6M8H51.1e-4244.94Probable sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 GN=Sesqu... [more]
Match NameE-valueIdentityDescription
A0A1S4DXZ83.9e-7579.89(-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103491132 PE=4 SV=1[more]
A0A0A0L7911.5e-7478.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G042380 PE=4 SV=1[more]
A0A0A0L5V37.3e-7476.92Terpene_synth_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G04... [more]
A0A6J1CP217.3e-7475.56valencene synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101340... [more]
A0A6J1CPK37.3e-7475.56(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G23960.19.6e-3436.11terpene synthase 21 [more]
AT5G23960.29.6e-3436.11terpene synthase 21 [more]
AT3G14540.15.2e-3236.93Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT1G31950.11.5e-3136.93Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT3G14520.11.7e-3037.28Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF19086Terpene_syn_C_2coord: 1..123
e-value: 1.1E-45
score: 155.9
NoneNo IPR availablePANTHERPTHR31225:SF102SUBFAMILY NOT NAMEDcoord: 2..171
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 2..171
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 1..178
e-value: 1.0E-66
score: 227.2
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 1..178

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003254.1HG10003254.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0010333 terpene synthase activity