HG10003234 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003234
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	(-)-germacrene D synthase-like
Location	Chr11: 19094730 .. 19098001 (+)
RNA-Seq Expression	HG10003234
Synteny	HG10003234

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAAATCCTTGCAATGTTGATGAAGAAGATGAAGATCTTTACATTATGGCTCTTAGATTTCGATTGCTTAGACAAGAAGGCTTCTTTGTTTCATGTGATAACTCTCTCTCAAGTGATTTAATTTTACCGTCGATTACTTTTTGCTAGAAGGAAATTTAAAATTTTAATTTATTTAGTAAATACTAAATTATGACAACTTTGGTTTATCATGTTTAATTGATAGTGTTTGGAAGTTTTGATCGGGTATAATAAGTCACTGGAGGCTTTCAATATTTTGTATTTAATAACTTTCTAAGGTCTCATTTAGTAATCATTTGATTTTTGGTTTTCAGTTTTTTAAAATTAAGCCTATTAATACCCCCTTTCGACTTCTAAATTTCTTGGTTTGTTATCTACTTTTTACCAATATTATAAAAAACTAAGCTAAATTTTGAAAACTAAAAAGAGTAGTTTATTTTTAAAACTTGTTTTTGTTTTTGAAATTTAGCTAAGAATTTCACTTTGTAGTTAAAAAGATGAAAATCATAGTAAAAAATTGAGAGAAAATAAATTTAATTTAAATAATAATAATAATAAAAAATAATTATCAAACAGGGCCTTAAAGTTTCAACTTCACCATTTCCAATTAATCCTTGTATAAAATATTTTCTTTTTTTTTGAACAAAATACAGGGAGGTGGGATAGGAGGTGCACCGGGACATCTCAACTAGGTTGACACATCCTTAGCATCCTCATCATAACCCGAATATTGCATTCATCGAAAGAAAAGTTCAGTACAAAGAGAGCTAGAGAATAGCTCGAAAAGAGAATGCAAGGGAGGCTATAGAAAAGCTCTCCAATTTAGTGCAGTGCTAGATACATCGTAATTACAAAAAAGAGAGGATTTAGAACTCCAAAGGGCCGCCAAAGAAAGAATATCATCCCACACTTGTGAAAGAGGTTTGTCCACTCCTTGAAAGGTTCGTCTGTTTCTCTCGAGCCATATACACCATAAGGAGGCATCAATGATGTTACTCCATATAATTCGGGCCTTGGAATTCAGATTGATTTGAATCAGAACAGTCATCAGGAAATCCAGTGTGGAAGGAGGTCCAAAATCCCCCTCAGTAATGTAGTCCCATATGCTGGAGGCAAAGTTGCAGTTGATGAAAATGTGTTCCATGGTCTCCGAATGCTTCCTACACAAGACACACCAATTTGGATTGAGATATATATTGGGATATCTTGATTGACATTTCTCCAAGGTATTGATGCCTTTTTGTAGAATGGTCCATAAAAAAAATTTACATTTGTTAGGCATAAGGGCAGACCACAAATTGCCAACATTGATACAATAGATATTGGATTGTACATTTTGTATATGCTCCTTGAGAGATTTCACACTGAAGAGTCCATCGGCTGCCAACTTCCATTGAATTTTATCTTTTTCTATAAGGTTCAGTGGGGTATCCCATGCATTGGACAAGTTATGCCAAGCTTCCCTCTCTCTTTGAAATAAGGGTCTGCGGGGGTGAAAGGACCATTCCAAGAGGTTGTTGTTCCATGCTTCAGATACAGTCAATAAAGGCTTATCAGAGAGAGCAAAGAAACGGGGTGCATATTTATCAAGAGCACCTTTTGTAGTCCAATTGTGGTACCAAAAGAGAGTGTTTTTGCCACTATTAATACGCCAAACCGCATGATCGAGAATGTGTGATTGTAGTTTGGCAATGTGGAACCATGGAGATTTGGAACTTTGACACTTGCAGATGGCAGGGATGGATTCAAGGCTGGTTGACTCATATTTTGAGTCAATAAGGGATCTCCAAAGACTATCCTTTTCATTGATATATCTCCATATCCACTTACTAAGGAGAGCAATATTTTTTGTTTTGATCTTATCAATGCCCAGACCCCCTTTGTCTTTGGTTTGGGTGACAGTGTCCCATCTCACAAGATGAGGATTGCCCCCTTTTTCTGTGCCCTCCCACAAAAAGTCTCTCATCAATTTCTCAACAGCTTTACAGATGGATGAGGGAGCGTGGAAGGTGGATAGATAATAGGTAGGCAGATTGCTGAGAACCGCTTGAAGGAGAGTTAATCTTCCACCTTTTGTAATGTAGGAGAACTTCCATGCAACAAATTTTTTCTCAATCTTTTCAATAATAGGAGCCCAAAAGGTAGGAGATTTTGGATTACCTCCTAGTGGCATACTAAGGTAAGAGATAGGGAATGAATTAGATTCACAGCCCCATATAGATTCCACTTGTCAAATACAATTGGGTTCGACGTTGATACCGGAGATAGAGGATTTTTGCAAGTTAATTTTCAGGCTAGAGATTTCTTCAAAAGTGCTGATAACGTGTCTGAGGTTTTGAACCTTTGATAGATCAGTCTCGGTGAAGAGTAAAATATCATCTGCAAAAAGGAGATGGGTGATTTGAAATTGTCCATTTTTAAGACTGAAGTCGGAGATCAAATCCTTTTGATGAGCCTTCTCAAGAACCATAGACAAATAGTCCATGGCTATTATGAAAAGAAAAGGGGATAGGGGATCTCCTTGCCGGATCCCTCTATTAGCTTGAATGTAACCTCTTGCCATTTAAAATGATGGAATATCCCACATTAGAGATACAAGCATTCATCCATTCAATCCATCTAGGATGGAAACCTTTATATTTTAACATAGAGAGAAGGAAAGACCAATTAACCTTGTCGAAAGGGGATAGGGGATCTCCTTGCCGGATCCCTCTATTAGCTTGTATAAGATATTTTCTAAAGTTGAAAAAGCTCAAAAATTGAAATTTCAAATTTTTACTACACATGCTGTTTAGGGTTTATGATGTATAAGGTACAACATTCAAAACTTAGAGATCTATTAAATATCTTAAGTCGGAAAAAAAATTAAACTCGTACTTTAACCTTCATTAAACTTTTAAACATGAGATTTCAAAGAATCAATTACAAAGGATGAAGTTGGTTCATTAAGTTTGTATGAAACATCCCACTTGACAATTAGAGGAGAAAAGATATTGGATGAAGCATTAGCTTTCACAACTACTCATCTTGAAGCAATGGCTATGAATTCCAACTCTCCATTTTCAGAAGAAGCTAAATATGCATTGAAGTGGCCAATTTATAAAACTATGCCAAGGTTGATGAGTAGGCACTATATCTCTCTCTATAACAACAATCCATTGAAGAATAATGTTTTGCTCACTTTTGCAAAGTTGGACTACAACTCTTTGCAAAAACTTTACCACAAAGAAGTGGGTGAATTTTCAAGGTGA

mRNA sequence

ATGCAAAATCCTTGCAATGTTGATGAAGAAGATGAAGATCTTTACATTATGGCTCTTAGATTTCGATTGCTTAGACAAGAAGGCTTCTTTGTTTCATGTGATAACTCTCTCTCAAATTTCAAAGAATCAATTACAAAGGATGAAGTTGGTTCATTAAGTTTGTATGAAACATCCCACTTGACAATTAGAGGAGAAAAGATATTGGATGAAGCATTAGCTTTCACAACTACTCATCTTGAAGCAATGGCTATGAATTCCAACTCTCCATTTTCAGAAGAAGCTAAATATGCATTGAAGTGGCCAATTTATAAAACTATGCCAAGGTTGATGAGTAGGCACTATATCTCTCTCTATAACAACAATCCATTGAAGAATAATGTTTTGCTCACTTTTGCAAAGTTGGACTACAACTCTTTGCAAAAACTTTACCACAAAGAAGTGGGTGAATTTTCAAGGTGA

Coding sequence (CDS)

ATGCAAAATCCTTGCAATGTTGATGAAGAAGATGAAGATCTTTACATTATGGCTCTTAGATTTCGATTGCTTAGACAAGAAGGCTTCTTTGTTTCATGTGATAACTCTCTCTCAAATTTCAAAGAATCAATTACAAAGGATGAAGTTGGTTCATTAAGTTTGTATGAAACATCCCACTTGACAATTAGAGGAGAAAAGATATTGGATGAAGCATTAGCTTTCACAACTACTCATCTTGAAGCAATGGCTATGAATTCCAACTCTCCATTTTCAGAAGAAGCTAAATATGCATTGAAGTGGCCAATTTATAAAACTATGCCAAGGTTGATGAGTAGGCACTATATCTCTCTCTATAACAACAATCCATTGAAGAATAATGTTTTGCTCACTTTTGCAAAGTTGGACTACAACTCTTTGCAAAAACTTTACCACAAAGAAGTGGGTGAATTTTCAAGGTGA

Protein sequence

MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCDNSLSNFKESITKDEVGSLSLYETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHYISLYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR

Homology

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BLAST of HG10003234 vs. NCBI nr
Match: KGN55986.2 (hypothetical protein Csa_011696 [Cucumis sativus])

HSP 1 Score: 238.8 bits (608), Expect = 3.1e-59
Identity = 119/158 (75.32%), Postives = 134/158 (84.81%), Query Frame = 0


Query: 1   MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSL 60
           M N  N+DEED DLY MALRFRLLRQ+GFFVSC+      N   +FKESITKDEVG LSL
Sbjct: 138 MHNASNLDEEDVDLYTMALRFRLLRQKGFFVSCEIFNKYTNESGDFKESITKDEVGLLSL 197

Query: 61  YETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHY 120
           YE SHL ++GE ILD+ALAFTTT L+A+AM+SNSPFS+E K++LKWPIYK MPR MSRHY
Sbjct: 198 YEASHLRMKGENILDKALAFTTTQLQAIAMDSNSPFSQEVKFSLKWPIYKAMPRFMSRHY 257

Query: 121 ISLYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           ISLY NNPLK+NVLLTFAKLDYNSLQKLY KE+GEFSR
Sbjct: 258 ISLYQNNPLKDNVLLTFAKLDYNSLQKLYQKELGEFSR 295

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BLAST of HG10003234 vs. NCBI nr
Match: XP_004151461.3 ((-)-germacrene D synthase [Cucumis sativus])

HSP 1 Score: 238.8 bits (608), Expect = 3.1e-59
Identity = 119/158 (75.32%), Postives = 134/158 (84.81%), Query Frame = 0


Query: 1   MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSL 60
           M N  N+DEED DLY MALRFRLLRQ+GFFVSC+      N   +FKESITKDEVG LSL
Sbjct: 93  MHNASNLDEEDVDLYTMALRFRLLRQKGFFVSCEIFNKYTNESGDFKESITKDEVGLLSL 152

Query: 61  YETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHY 120
           YE SHL ++GE ILD+ALAFTTT L+A+AM+SNSPFS+E K++LKWPIYK MPR MSRHY
Sbjct: 153 YEASHLRMKGENILDKALAFTTTQLQAIAMDSNSPFSQEVKFSLKWPIYKAMPRFMSRHY 212

Query: 121 ISLYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           ISLY NNPLK+NVLLTFAKLDYNSLQKLY KE+GEFSR
Sbjct: 213 ISLYQNNPLKDNVLLTFAKLDYNSLQKLYQKELGEFSR 250

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BLAST of HG10003234 vs. NCBI nr
Match: XP_031738277.1 ((-)-germacrene D synthase isoform X2 [Cucumis sativus])

HSP 1 Score: 237.7 bits (605), Expect = 6.8e-59
Identity = 119/158 (75.32%), Postives = 131/158 (82.91%), Query Frame = 0


Query: 1   MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSL 60
           M+NPCNVD+E EDLYI ALRFRLLRQ+GFFVSC+      N     KESI KDE G LSL
Sbjct: 109 MKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRKDENGLLSL 168

Query: 61  YETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHY 120
           YE SHL ++GE ILDEALAFTTTHL+AMAM+SNSPF EEAKYALKWPIYK + R M+RHY
Sbjct: 169 YEASHLRMKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHY 228

Query: 121 ISLYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           ISLY+NNPLKNN LLTFAKLDYNSLQKLY KE+ E SR
Sbjct: 229 ISLYSNNPLKNNALLTFAKLDYNSLQKLYQKELSELSR 266

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BLAST of HG10003234 vs. NCBI nr
Match: XP_011650452.2 ((-)-germacrene D synthase isoform X1 [Cucumis sativus] >XP_031738276.1 (-)-germacrene D synthase isoform X1 [Cucumis sativus] >KAE8650088.1 hypothetical protein Csa_009890 [Cucumis sativus])

HSP 1 Score: 237.7 bits (605), Expect = 6.8e-59
Identity = 119/158 (75.32%), Postives = 131/158 (82.91%), Query Frame = 0


Query: 1   MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSL 60
           M+NPCNVD+E EDLYI ALRFRLLRQ+GFFVSC+      N     KESI KDE G LSL
Sbjct: 3   MKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRKDENGLLSL 62

Query: 61  YETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHY 120
           YE SHL ++GE ILDEALAFTTTHL+AMAM+SNSPF EEAKYALKWPIYK + R M+RHY
Sbjct: 63  YEASHLRMKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHY 122

Query: 121 ISLYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           ISLY+NNPLKNN LLTFAKLDYNSLQKLY KE+ E SR
Sbjct: 123 ISLYSNNPLKNNALLTFAKLDYNSLQKLYQKELSELSR 160

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BLAST of HG10003234 vs. NCBI nr
Match: XP_038877743.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 236.9 bits (603), Expect = 1.2e-58
Identity = 120/159 (75.47%), Postives = 134/159 (84.28%), Query Frame = 0


Query: 1   MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSL 60
           +QNP NVDEEDEDLYI ALRFRLLRQ+GFFVSCD      N + +FKESI KD  G +SL
Sbjct: 103 IQNPTNVDEEDEDLYITALRFRLLRQQGFFVSCDIFNKFTNEMGDFKESIVKDGAGXISL 162

Query: 61  YETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHY 120
           YE SHL ++GE ILDEALAFTTT L+AMAM+S+SPFSEEAKYALKWPIYK MPR M+RHY
Sbjct: 163 YEASHLRMKGENILDEALAFTTTQLKAMAMDSSSPFSEEAKYALKWPIYKAMPRFMNRHY 222

Query: 121 ISLYNNNPLK-NNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           ISLY NN LK NNVLL FAKLD+N+LQKLY KE+GEFSR
Sbjct: 223 ISLYQNNALKNNNVLLKFAKLDFNTLQKLYQKELGEFSR 261

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BLAST of HG10003234 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.1e-35
Identity = 77/152 (50.66%), Postives = 105/152 (69.08%), Query Frame = 0


Query: 6   NVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSLYETSH 65
           +V   +ED+Y  +LRFRLLRQ+G+ VSCD      ++  NFKES++ D  G LSLYE +H
Sbjct: 106 SVVSAEEDVYTASLRFRLLRQQGYHVSCDLFNNFKDNEGNFKESLSSDVRGMLSLYEATH 165

Query: 66  LTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHYISLYN 125
             + GE ILDEALAFTTTHL++   +S++P +E+  +ALK PI K +PRL +RHY S+Y 
Sbjct: 166 FRVHGEDILDEALAFTTTHLQSATKHSSNPLAEQVVHALKQPIRKGLPRLEARHYFSVYQ 225

Query: 126 NNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFS 152
            +   N  LL  AKLD+N LQKL+ KE+ + S
Sbjct: 226 ADDSHNKALLKLAKLDFNLLQKLHQKELSDIS 257

[Close]

BLAST of HG10003234 vs. ExPASy Swiss-Prot
Match: Q9FQ27 ((E,E)-germacrene B synthase OS=Solanum habrochaites OX=62890 GN=SSTLH1 PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.1e-32
Identity = 74/161 (45.96%), Postives = 110/161 (68.32%), Query Frame = 0


Query: 1   MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSL 60
           +QN  +  +++ +L+I++LRFRL+RQ+G ++S D      N    FKE++T D  G LSL
Sbjct: 84  IQNIFDAPKQNNNLHIVSLRFRLVRQQGHYMSSDVFKQFTNQDGKFKETLTNDVQGLLSL 143

Query: 61  YETSHLTIRGEKILDEALAFTTTHLEAMA---MNSNSPFSEEAKYALKWPIYKTMPRLMS 120
           YE S+L +R E+IL+EALAFTTTHL+++     N+N+    E   AL  PI  T+PR+ +
Sbjct: 144 YEASYLRVRDEEILEEALAFTTTHLKSIVSTMSNNNNSLKVEVSEALTQPIRMTLPRMEA 203

Query: 121 RHYISLYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           R YIS+Y NN   N++LL FAKLD+N LQKL+ +E+ + +R
Sbjct: 204 RRYISIYENNDAHNHLLLKFAKLDFNMLQKLHQRELSDLTR 244

[Close]

BLAST of HG10003234 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.9e-32
Identity = 76/156 (48.72%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 6   NVDEEDEDLYIMALRFRLLRQEGFFVSCDNSL------SNFKESITKDEVGSLSLYETSH 65
           N +   EDL+I AL FRLLRQ+G+ +SCD  L        FKES+ +DE G LSLYE SH
Sbjct: 116 NKNSNGEDLHITALLFRLLRQQGYRISCDIFLKFMDDNGKFKESLVEDERGLLSLYEASH 175

Query: 66  LTIRGEKILDEALAFTTTHLEAMA---MNSNSPFSEEAKYALKWPIYKTMPRLMSRHYIS 125
           +   GE +L+EAL FTTTHL+       N N  F+ E   ALK PI K++PR+ +R Y+ 
Sbjct: 176 MMGHGEALLEEALEFTTTHLQTYIHRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLE 235

Query: 126 LYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           +Y  +P  N  LL F+KLD+N LQKL+ KE+ E  R
Sbjct: 236 IYQQHPSHNETLLEFSKLDFNILQKLHQKELSEICR 271

[Close]

BLAST of HG10003234 vs. ExPASy Swiss-Prot
Match: Q39760 ((+)-delta-cadinene synthase isozyme XC14 OS=Gossypium arboreum OX=29729 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.3e-32
Identity = 72/153 (47.06%), Postives = 99/153 (64.71%), Query Frame = 0


Query: 6   NVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSLYETSH 65
           N ++ + DLY  +LRFRLLR+ GF VSCD      +   NFK S+T D  G L LY+ S+
Sbjct: 103 NNNDAENDLYTTSLRFRLLREHGFNVSCDVFNKFKDEQGNFKSSVTSDVRGLLELYQASY 162

Query: 66  LTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHYISLYN 125
           L + GE ILDEA++FTT HL     + + P SEE  +ALK  I + +PR+ +RHY+S+Y 
Sbjct: 163 LRVHGEDILDEAISFTTNHLSLAVASLDYPLSEEVSHALKQSIRRGLPRVEARHYLSVYQ 222

Query: 126 NNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           +    N VLL FAK+D+N +Q L+ KE+ E SR
Sbjct: 223 DIESHNKVLLEFAKIDFNMVQLLHRKELSEISR 255

[Close]

BLAST of HG10003234 vs. ExPASy Swiss-Prot
Match: P93665 ((+)-delta-cadinene synthase OS=Gossypium hirsutum OX=3635 GN=CDN1 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.3e-32
Identity = 71/153 (46.41%), Postives = 99/153 (64.71%), Query Frame = 0


Query: 6   NVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSLYETSH 65
           N ++ + DLY  +LRFRLLR+ GF VSCD      +   NFK S+T D  G L LY+ S+
Sbjct: 103 NNNDAENDLYTTSLRFRLLREHGFHVSCDVFNKFKDEQGNFKSSVTSDVRGLLELYQASY 162

Query: 66  LTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHYISLYN 125
           L + GE ILDEA++FT+ HL     + + P SEE  +ALK  I + +PR+ +RHY+S+Y 
Sbjct: 163 LRVHGEDILDEAISFTSNHLSLAVASLDHPLSEEVSHALKQSIRRGLPRVEARHYLSVYQ 222

Query: 126 NNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           +    N VLL FAK+D+N +Q L+ KE+ E SR
Sbjct: 223 DIESHNKVLLEFAKIDFNMVQLLHRKELSEISR 255

[Close]

BLAST of HG10003234 vs. ExPASy TrEMBL
Match: A0A0A0L2M0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G040870 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.5e-59
Identity = 119/158 (75.32%), Postives = 134/158 (84.81%), Query Frame = 0


Query: 1   MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSL 60
           M N  N+DEED DLY MALRFRLLRQ+GFFVSC+      N   +FKESITKDEVG LSL
Sbjct: 53  MHNASNLDEEDVDLYTMALRFRLLRQKGFFVSCEIFNKYTNESGDFKESITKDEVGLLSL 112

Query: 61  YETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHY 120
           YE SHL ++GE ILD+ALAFTTT L+A+AM+SNSPFS+E K++LKWPIYK MPR MSRHY
Sbjct: 113 YEASHLRMKGENILDKALAFTTTQLQAIAMDSNSPFSQEVKFSLKWPIYKAMPRFMSRHY 172

Query: 121 ISLYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           ISLY NNPLK+NVLLTFAKLDYNSLQKLY KE+GEFSR
Sbjct: 173 ISLYQNNPLKDNVLLTFAKLDYNSLQKLYQKELGEFSR 210

[Close]

BLAST of HG10003234 vs. ExPASy TrEMBL
Match: A0A1S3BLW6 ((-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490985 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 9.6e-59
Identity = 116/158 (73.42%), Postives = 133/158 (84.18%), Query Frame = 0


Query: 1   MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSL 60
           MQNPCN+DEE EDLYI ALRFRLLRQ+G F+SC+      N    FKESI KD++G LSL
Sbjct: 110 MQNPCNIDEEHEDLYITALRFRLLRQQGLFISCEIFNKFTNERGEFKESIGKDKIGLLSL 169

Query: 61  YETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHY 120
           YE SHL ++GE ILDEALAFTT++L+AMAM+SNSPF EEAKYALKWPIYK + RLM+RHY
Sbjct: 170 YEASHLRMKGENILDEALAFTTSYLKAMAMDSNSPFYEEAKYALKWPIYKAVKRLMARHY 229

Query: 121 ISLYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           ISLY+NNPLKNNVLLTFAKLDYNSLQKLY  E+ E +R
Sbjct: 230 ISLYSNNPLKNNVLLTFAKLDYNSLQKLYQNELSELTR 267

[Close]

BLAST of HG10003234 vs. ExPASy TrEMBL
Match: A0A0A0L782 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G040850 PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.6e-58
Identity = 118/158 (74.68%), Postives = 130/158 (82.28%), Query Frame = 0


Query: 1   MQNPCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSL 60
           M+NPCNVD+E EDLYI ALRFRLLRQ+GFFVSC+      N     KESI KDE   LSL
Sbjct: 109 MKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRKDENSLLSL 168

Query: 61  YETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHY 120
           YE SHL ++GE ILDEALAFTTTHL+AMAM+SNSPF EEAKYALKWPIYK + R M+RHY
Sbjct: 169 YEASHLRMKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHY 228

Query: 121 ISLYNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           ISLY+NNPLKNN LLTFAKLDYNSLQKLY KE+ E SR
Sbjct: 229 ISLYSNNPLKNNALLTFAKLDYNSLQKLYQKELSELSR 266

[Close]

BLAST of HG10003234 vs. ExPASy TrEMBL
Match: A0A0A0LN25 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G427840 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 1.1e-54
Identity = 115/160 (71.88%), Postives = 134/160 (83.75%), Query Frame = 0


Query: 1   MQNPCNVDEED-EDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLS 60
           +QN  +VDEED EDLYI+AL+FRLLRQ GFFVSC+      +   +FKESITKDE G LS
Sbjct: 106 IQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVSCEILKKFTSESGDFKESITKDERGLLS 165

Query: 61  LYETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRH 120
           LYE SHL ++GE I+DEAL FT+T LEAMAM+S SPFSEEAKYALKWPIYKT+PR M+R+
Sbjct: 166 LYEASHLRMKGENIMDEALEFTSTQLEAMAMDSTSPFSEEAKYALKWPIYKTLPRFMTRN 225

Query: 121 YISLYNNNPL-KNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           YISLY NNPL K+N+LLTFAKLDYNSLQKLY +E+GE SR
Sbjct: 226 YISLYRNNPLKKSNILLTFAKLDYNSLQKLYQRELGELSR 265

[Close]

BLAST of HG10003234 vs. ExPASy TrEMBL
Match: A0A5D3BQ16 (Putative terpene synthase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G00170 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 9.3e-54
Identity = 114/161 (70.81%), Postives = 132/161 (81.99%), Query Frame = 0


Query: 1   MQNPCNVDEED-EDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLS 60
           +QN  +VDEED EDLYI+AL+FRLLRQ+GF VSC+          +FKESITKDE G LS
Sbjct: 108 IQNSAHVDEEDEEDLYIIALKFRLLRQQGFPVSCEIFNKFTTESGDFKESITKDEKGLLS 167

Query: 61  LYETSHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRH 120
           LYE SHL ++GE I+DEAL FTTT LEAMAM+S SPFS+EAKYALKWPIYKT+ R M+RH
Sbjct: 168 LYEASHLRMKGESIMDEALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRH 227

Query: 121 YISLYNNNPLK--NNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           YISLY+NNP K  NN+LLTFAKLDYNSLQKLY +E+GE SR
Sbjct: 228 YISLYHNNPTKNDNNILLTFAKLDYNSLQKLYQRELGELSR 268

[Close]

BLAST of HG10003234 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 97.8 bits (242), Expect = 7.8e-21
Identity = 59/155 (38.06%), Postives = 86/155 (55.48%), Query Frame = 0


Query: 4   PCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSLYET 63
           P  V +E  DLY + + F++ RQ GF +S D      +    FK  +  D  G LSLYE 
Sbjct: 93  PQMVRQEGCDLYTVGIIFQVFRQFGFKLSADVFEKFKDENGKFKGHLVTDAYGMLSLYEA 152

Query: 64  SHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHYISL 123
           +     GE I+DEALAF+ +HLE ++  S+   +   K ALK P +K + R+ +R YIS 
Sbjct: 153 AQWGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETRQYISY 212

Query: 124 YNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           Y      +  LL FAK+D+N LQ L+ +E+   +R
Sbjct: 213 YEEEESCDPTLLEFAKIDFNLLQILHREELACVTR 247

[Close]

BLAST of HG10003234 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 97.8 bits (242), Expect = 7.8e-21
Identity = 59/155 (38.06%), Postives = 86/155 (55.48%), Query Frame = 0


Query: 4   PCNVDEEDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSLYET 63
           P  V +E  DLY + + F++ RQ GF +S D      +    FK  +  D  G LSLYE 
Sbjct: 93  PQMVRQEGCDLYTVGIIFQVFRQFGFKLSADVFEKFKDENGKFKGHLVTDAYGMLSLYEA 152

Query: 64  SHLTIRGEKILDEALAFTTTHLEAMAMNSNSPFSEEAKYALKWPIYKTMPRLMSRHYISL 123
           +     GE I+DEALAF+ +HLE ++  S+   +   K ALK P +K + R+ +R YIS 
Sbjct: 153 AQWGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETRQYISY 212

Query: 124 YNNNPLKNNVLLTFAKLDYNSLQKLYHKEVGEFSR 153
           Y      +  LL FAK+D+N LQ L+ +E+   +R
Sbjct: 213 YEEEESCDPTLLEFAKIDFNLLQILHREELACVTR 247

[Close]

BLAST of HG10003234 vs. TAIR 10
Match: AT3G29190.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 4.0e-17
Identity = 52/145 (35.86%), Postives = 82/145 (56.55%), Query Frame = 0


Query: 11  DEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSLYETSHLTIRG 70
           +EDLY +++ F + R+ G ++S D       +  NFK+S+  D  G LS YE +++    
Sbjct: 151 EEDLYTVSIIFWVFRRYGHYISSDFFRRFKGNDGNFKKSLIGDAKGMLSFYEAANMATTK 210

Query: 71  EKILDEALAFTTTHLEAMAMNSNSP--FSEEAKYALKWPIYKTMPRLMSRHYISLYNNNP 130
           + ILDEAL+FT++HLE++A N   P   S   + AL    +  M  L++  YIS Y    
Sbjct: 211 DYILDEALSFTSSHLESLAANGACPPHMSRRIRNALNASQHWNMEMLVAVEYISFYEKEK 270

Query: 131 LKNNVLLTFAKLDYNSLQKLYHKEV 148
             N +LL F+KL++  LQ  Y +E+
Sbjct: 271 DHNEMLLKFSKLNFKFLQLQYLQEL 295

[Close]

BLAST of HG10003234 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 84.7 bits (208), Expect = 6.9e-17
Identity = 55/148 (37.16%), Postives = 81/148 (54.73%), Query Frame = 0


Query: 10  EDEDLYIMALRFRLLRQEGFFVSCD------NSLSNFKESITKDEVGSLSLYETSHLTIR 69
           E++DL  +++ F + R  G  +SCD           FKES+ KD  G L L+E +HL   
Sbjct: 149 EEDDLETISIMFEVFRLYGHKMSCDAFDRFRGGDGRFKESLAKDVRGMLQLFEVAHLGTL 208

Query: 70  GEKILDEALAFTTTHLEAMA---MNSNSP-FSEEAKYALKWPIYKTMPRLMSRHYISLYN 129
            E I+DEAL FT  HLE++    ++S SP   +  + +L  P Y  +  L++R YIS Y 
Sbjct: 209 SEDIMDEALRFTRNHLESLTSGNVSSASPHILKHIQNSLYIPRYCNIEVLVAREYISYYE 268

Query: 130 NNPLKNNVLLTFAKLDYNSLQKLYHKEV 148
                N +LL FAKL++N  Q  Y +E+
Sbjct: 269 QEEGYNEILLKFAKLNFNFCQCHYIQEI 296

[Close]

BLAST of HG10003234 vs. TAIR 10
Match: AT4G16740.1 (terpene synthase 03 )

HSP 1 Score: 79.7 bits (195), Expect = 2.2e-15
Identity = 54/145 (37.24%), Postives = 80/145 (55.17%), Query Frame = 0


Query: 13  DLYIMALRFRLLRQEGFFVSCDNSLSNFKESI-TKDEVGSLSLYETSHLTIRGEKILDEA 72
           DLY  AL FRLLRQ GF ++ D    N    +  KD  G LSLYE S+L+ R +  L E+
Sbjct: 123 DLYATALEFRLLRQHGFSIAQDVFDGNIGVDLDDKDIKGILSLYEASYLSTRIDTKLKES 182

Query: 73  LAFTTTHL-EAMAMNSNSPFSEEAK----YALKWPIYKTMPRLMSRHYISLYNNNPLKNN 132
           + +TT  L + + +N N   S   +    +AL+ P ++ + RL +R YI +Y      N 
Sbjct: 183 IYYTTKRLRKFVEVNKNETKSYTLRRMVIHALEMPYHRRVGRLEARWYIEVYGERHDMNP 242

Query: 133 VLLTFAKLDYNSLQKLYHKEVGEFS 152
           +LL  AKLD+N +Q ++  E+   S
Sbjct: 243 ILLELAKLDFNFVQAIHQDELKSLS 267

The following BLAST results are available for this feature:

BLAST of HG10003234 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN55986.2	3.1e-59	75.32	hypothetical protein Csa_011696 [Cucumis sativus]	[more]
XP_004151461.3	3.1e-59	75.32	(-)-germacrene D synthase [Cucumis sativus]	[more]
XP_031738277.1	6.8e-59	75.32	(-)-germacrene D synthase isoform X2 [Cucumis sativus]	[more]
XP_011650452.2	6.8e-59	75.32	(-)-germacrene D synthase isoform X1 [Cucumis sativus] >XP_031738276.1 (-)-germa...	[more]
XP_038877743.1	1.2e-58	75.47	LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida]	[more]

BLAST of HG10003234 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6Q3H3	1.1e-35	50.66	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]
Q9FQ27	1.1e-32	45.96	(E,E)-germacrene B synthase OS=Solanum habrochaites OX=62890 GN=SSTLH1 PE=1 SV=1	[more]
B2KSJ5	1.9e-32	48.72	(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q39760	3.3e-32	47.06	(+)-delta-cadinene synthase isozyme XC14 OS=Gossypium arboreum OX=29729 PE=2 SV=...	[more]
P93665	4.3e-32	46.41	(+)-delta-cadinene synthase OS=Gossypium hirsutum OX=3635 GN=CDN1 PE=1 SV=1	[more]

BLAST of HG10003234 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2M0	1.5e-59	75.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G040870 PE=4 SV=1	[more]
A0A1S3BLW6	9.6e-59	73.42	(-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490985 PE=4 SV=1	[more]
A0A0A0L782	1.6e-58	74.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G040850 PE=4 SV=1	[more]
A0A0A0LN25	1.1e-54	71.88	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G427840 PE=4 SV=1	[more]
A0A5D3BQ16	9.3e-54	70.81	Putative terpene synthase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of HG10003234 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23960.1	7.8e-21	38.06	terpene synthase 21	[more]
AT5G23960.2	7.8e-21	38.06	terpene synthase 21	[more]
AT3G29190.1	4.0e-17	35.86	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G14540.1	6.9e-17	37.16	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT4G16740.1	2.2e-15	37.24	terpene synthase 03	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036965	Terpene synthase, N-terminal domain superfamily	GENE3D	1.50.10.130		coord: 8..123 e-value: 3.2E-46 score: 159.8
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 8..98 e-value: 3.8E-23 score: 82.2
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 124..152 e-value: 3.2E-46 score: 159.8
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 10..152
None	No IPR available	PANTHER	PTHR31225:SF111	TERPENE SYNTHASE 2-RELATED	coord: 10..152
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 6..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10003234.1	HG10003234.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0016829	lyase activity