HG10003229 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10003229 vs. NCBI nr
Match: XP_011650446.1 (uncharacterized protein LOC101214338 isoform X2 [Cucumis sativus])

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 541/613 (88.25%), Postives = 568/613 (92.66%), Query Frame = 0

Query: 9   MALTPTVIAANCF-FFFFFVFFAVPVRSSISPDFSSFHQCSCPRFTPMDSKKDNECLENV 68
           MA TPT+   NCF  FFFF FF V VRSSIS  FSSF QCS  RFT MDSKKD+ECLEN+
Sbjct: 1   MASTPTI---NCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMDSKKDSECLENM 60

Query: 69  GKTSLLKCAFGAFDADFFNNTKMDEVLKGAKEFNLPTIRANQKLVASENGGLHNPSPLVF 128
           G+TS+LKC F A DADFFNNTK DEVLKGA+E N+P IRAN+KLVASENGGLHNPS LVF
Sbjct: 61  GRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVF 120

Query: 129 NPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMSVLELGELIKTKQISSQELVRIFL 188
           NPEWANE  RHKSKRFCYPPVSG+KRPNNE+DIAFMSVLELGELIKT+QISSQELVRIFL
Sbjct: 121 NPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFL 180

Query: 189 QRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTT 248
           QRLKRY+HVL+AVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTT
Sbjct: 181 QRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTT 240

Query: 249 WGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEQFSTG 308
           WGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIE+FSTG
Sbjct: 241 WGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTG 300

Query: 309 SSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGT-------------DKL 368
           SSAGPAACTSAGMVPFAIGSET GSMTYPAARCGVTAIRPTFGT             DKL
Sbjct: 301 SSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDKL 360

Query: 369 GPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYLDDADKEVVRVLS 428
           GPFCRNAVDCAV+LDVIRGKDPHDLSSVES LDDPFSIDISKLTVGYLDDAD EVVR+LS
Sbjct: 361 GPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVRILS 420

Query: 429 SKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRAR 488
           SKGVNM+PFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRAR
Sbjct: 421 SKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRAR 480

Query: 489 LISAIDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPVIVIPTGFKNI 548
           LI A+DYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLP++V+PTGFKNI
Sbjct: 481 LIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPIVVVPTGFKNI 540

Query: 549 SNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPIDDLGPDDVIPD 608
           SNPPST TTRR++ ITTGIYA PH+DHIALALAMAYQSATDHHRA+PPIDDLGP DV+PD
Sbjct: 541 SNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPIDDLGPSDVLPD 600

BLAST of HG10003229 vs. NCBI nr
Match: XP_011650445.1 (uncharacterized protein LOC101214338 isoform X1 [Cucumis sativus] >KAE8650086.1 hypothetical protein Csa_011729 [Cucumis sativus])

HSP 1 Score: 1071.2 bits (2769), Expect = 3.3e-309
Identity = 541/639 (84.66%), Postives = 568/639 (88.89%), Query Frame = 0

Query: 9   MALTPTVIAANCF-FFFFFVFFAVPVRSSISPDFSSFHQCSCPRFTPM------------ 68
           MA TPT+   NCF  FFFF FF V VRSSIS  FSSF QCS  RFT M            
Sbjct: 1   MASTPTI---NCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTYKKFWKLLY 60

Query: 69  --------------DSKKDNECLENVGKTSLLKCAFGAFDADFFNNTKMDEVLKGAKEFN 128
                         DSKKD+ECLEN+G+TS+LKC F A DADFFNNTK DEVLKGA+E N
Sbjct: 61  EKVGVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELN 120

Query: 129 LPTIRANQKLVASENGGLHNPSPLVFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIA 188
           +P IRAN+KLVASENGGLHNPS LVFNPEWANE  RHKSKRFCYPPVSG+KRPNNE+DIA
Sbjct: 121 VPIIRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIA 180

Query: 189 FMSVLELGELIKTKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQ 248
           FMSVLELGELIKT+QISSQELVRIFLQRLKRY+HVL+AVVSFTEELAYKQAKEADELFAQ
Sbjct: 181 FMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQ 240

Query: 249 GVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVT 308
           GVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVT
Sbjct: 241 GVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVT 300

Query: 309 GSLAYDDIWFGGRTRNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCG 368
           GSLAYDDIWFGGRTRNPWNIE+FSTGSSAGPAACTSAGMVPFAIGSET GSMTYPAARCG
Sbjct: 301 GSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCG 360

Query: 369 VTAIRPTFGT-------------DKLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDD 428
           VTAIRPTFGT             DKLGPFCRNAVDCAV+LDVIRGKDPHDLSSVES LDD
Sbjct: 361 VTAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDD 420

Query: 429 PFSIDISKLTVGYLDDADKEVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHF 488
           PFSIDISKLTVGYLDDAD EVVR+LSSKGVNM+PFNLSYSVDSVQGI+NFTMDVDMLAHF
Sbjct: 421 PFSIDISKLTVGYLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHF 480

Query: 489 DEWQRLGLDDEYEAQDQWPTELRRARLISAIDYVQAQRARGKLIREVRESFNVDALIGNA 548
           DEWQRLGLDDEYEAQDQWPTELRRARLI A+DYVQAQRARGKLIREVRESFNVDALIGNA
Sbjct: 481 DEWQRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNA 540

Query: 549 TDWEKVCMGNLVGLPVIVIPTGFKNISNPPSTATTRRRSAITTGIYAAPHKDHIALALAM 608
           TDWEKVCMGNLVGLP++V+PTGFKNISNPPST TTRR++ ITTGIYA PH+DHIALALAM
Sbjct: 541 TDWEKVCMGNLVGLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAM 600

BLAST of HG10003229 vs. NCBI nr
Match: XP_038905194.1 (glutamyl-tRNA(Gln) amidotransferase subunit A [Benincasa hispida])

HSP 1 Score: 1050.0 bits (2714), Expect = 7.6e-303
Identity = 540/636 (84.91%), Postives = 558/636 (87.74%), Query Frame = 0

Query: 9   MALTPTVIAANCFFFFFFVFFAVPVRSSISPDFSSFHQCSCPRFTPM------------- 68
           MA   +VIA N FFFFFF FFAVP     SP F      SCPRFTPM             
Sbjct: 1   MASIRSVIAVN-FFFFFFFFFAVP----SSPIFHVLPSNSCPRFTPMMGTYKKFWKLMCE 60

Query: 69  -----------DSKKDNECLENVGKTSLLKCAFGAFDADFFNNTKMDEVLKGAKEFNLPT 128
                       SKKD EC   VGKTSLLKC F AFDADFFNNTKMDEVLKGA+EFNLPT
Sbjct: 61  KVGVHSHTPPFCSKKDTEC---VGKTSLLKCVFRAFDADFFNNTKMDEVLKGAEEFNLPT 120

Query: 129 IRANQKLVASENGGLHNPSPLVFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMS 188
           IRAN+KLVAS NGGLHNPSPLVFNPEWANE  RHKSKRFCYP VSGVKRP NEEDIAFMS
Sbjct: 121 IRANRKLVASRNGGLHNPSPLVFNPEWANENIRHKSKRFCYPSVSGVKRPKNEEDIAFMS 180

Query: 189 VLELGELIKTKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVY 248
           VLELGELIKTKQISSQELVRIFLQRLKRY+HVLKAVVSFTEELAYKQAKEADELFA+G Y
Sbjct: 181 VLELGELIKTKQISSQELVRIFLQRLKRYNHVLKAVVSFTEELAYKQAKEADELFARGTY 240

Query: 249 LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL 308
           LGPLHGIPYGLKDIISVPGY+TTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL
Sbjct: 241 LGPLHGIPYGLKDIISVPGYETTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL 300

Query: 309 AYDDIWFGGRTRNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTA 368
           AYDDIWFGGRTRNPWNIE+F+TGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTA
Sbjct: 301 AYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTA 360

Query: 369 IRPTFGT-------------DKLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFS 428
           IRPTFGT             DKLGPFCR+AVDC VILDVIRGKDPHDLSSVES L+DPFS
Sbjct: 361 IRPTFGTVGRSGVMSISESLDKLGPFCRSAVDCTVILDVIRGKDPHDLSSVESSLEDPFS 420

Query: 429 IDISKLTVGYLDDADKEVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEW 488
           +DISKLTVGYLDDA+ EVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEW
Sbjct: 421 VDISKLTVGYLDDAEMEVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEW 480

Query: 489 QRLGLDDEYEAQDQWPTELRRARLISAIDYVQAQRARGKLIREVRESFNVDALIGNATDW 548
           QRLGLDDEYEAQDQWPTELRRARLI A+DYVQAQRARGKLI+EVRE FNVDALIGNATDW
Sbjct: 481 QRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIKEVRERFNVDALIGNATDW 540

Query: 549 EKVCMGNLVGLPVIVIPTGFKNISNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQ 608
           E+VCMGNLVGLPVIV+PTGFKNISNPPST  TRRR+ ITTGIYA PHKDHIALALAMAYQ
Sbjct: 541 ERVCMGNLVGLPVIVVPTGFKNISNPPSTGPTRRRTTITTGIYAPPHKDHIALALAMAYQ 600

BLAST of HG10003229 vs. NCBI nr
Match: XP_023540353.1 (uncharacterized protein LOC111800751 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.9e-301
Identity = 526/622 (84.57%), Postives = 563/622 (90.51%), Query Frame = 0

Query: 1   MPIDKCPPMALTPTVIAANCFFFFFFV--FFAVPVRSSISPDFSSFHQCSCPRFTPMDSK 60
           MPIDK   M  T T +A+NCFFF FFV  FFAVP+RSSISP+FSS  Q S P FT MD  
Sbjct: 1   MPIDKWLSMPATRTTVASNCFFFVFFVFIFFAVPLRSSISPNFSSSDQ-SRPCFTAMDRN 60

Query: 61  KDNECLENVGKTSLLKCAFGAFDADFFNNTKMDEVLKGAKEFNLPTIRANQKLVASENGG 120
           KD E  +N GK S     F AFDADFF+NT+MDEVLKGA+EFNLP IRAN+KLVASENGG
Sbjct: 61  KDTESFKNGGKRSF----FRAFDADFFSNTEMDEVLKGAEEFNLPVIRANRKLVASENGG 120

Query: 121 LHNPSPLVFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMSVLELGELIKTKQIS 180
           LHNPSPLVF+  WANE+F+HKS+RFCYPPVSGVKRP NEEDIAFM+VLELGELIKTKQI+
Sbjct: 121 LHNPSPLVFSSNWANERFQHKSRRFCYPPVSGVKRPKNEEDIAFMNVLELGELIKTKQIT 180

Query: 181 SQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDI 240
           SQELV IFLQRLKRYD+VL+AVVSFTE+LAYKQAKEADELFAQGVYLGPLHGIPYGLKDI
Sbjct: 181 SQELVGIFLQRLKRYDYVLQAVVSFTEDLAYKQAKEADELFAQGVYLGPLHGIPYGLKDI 240

Query: 241 ISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNP 300
           ISVPGYKTTWGSKSFKEQVIDVEAWVY+QLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNP
Sbjct: 241 ISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNP 300

Query: 301 WNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGT------- 360
           WNIE+F+TGSSAGPAACTSAG+VPFAIGSETAGSMTYPA+RCGVTA+RPTFGT       
Sbjct: 301 WNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTYPASRCGVTALRPTFGTVGRSGVM 360

Query: 361 ------DKLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYLDDA 420
                 DKLGPFCRNA+DCAVILDVIRGKDPHDLSSVES LDDPFSIDISKLTVGYLDDA
Sbjct: 361 SISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDA 420

Query: 421 DKEVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQ 480
           D EVVRVLSSKG NM+PFNL+YS+DSVQGILNFTMD+DMLAHFDEWQR  LD EYEAQDQ
Sbjct: 421 DMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLAHFDEWQRSRLDHEYEAQDQ 480

Query: 481 WPTELRRARLISAIDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPVI 540
           WPTELRRARLI A+DYVQAQRARGKLIREV+ESFNVDALIGNATDWE+VCMGNLVGLPV+
Sbjct: 481 WPTELRRARLIPAVDYVQAQRARGKLIREVKESFNVDALIGNATDWERVCMGNLVGLPVM 540

Query: 541 VIPTGFKNISNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPIDD 600
           V+PTGFKNISNPP+T TTRRR+AITTGIYA PHKDHIALALAMAYQSATDHHRARPPIDD
Sbjct: 541 VVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAYQSATDHHRARPPIDD 600

Query: 601 LGPDDVIPDPPLVSIPPRLLHL 608
           LGP D IP+PPLV IPPRLLHL
Sbjct: 601 LGPHDAIPEPPLVPIPPRLLHL 617

BLAST of HG10003229 vs. NCBI nr
Match: TYK19429.1 (glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo var. makuwa])

HSP 1 Score: 1042.3 bits (2694), Expect = 1.6e-300
Identity = 510/559 (91.23%), Postives = 537/559 (96.06%), Query Frame = 0

Query: 54  PMDSKKDNECLENVGKTSLLKCAFGAFDADFFNNTKMDEVLKGAKEFNLPTIRANQKLVA 113
           P  SKKD+ECLEN+G+TS+LKC F AFDADFFNNTK DEVLKGA+E N+PTIRAN+KLVA
Sbjct: 12  PFCSKKDSECLENMGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVA 71

Query: 114 SENGGLHNPSPLVFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMSVLELGELIK 173
           SENGGLHNPSPLVFNPEWANE  RHKSKRFCYPPV GVKRPNNE+DIAFMSVLELGELIK
Sbjct: 72  SENGGLHNPSPLVFNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIK 131

Query: 174 TKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 233
           T+QISSQELVRIFLQRLKRY+HVL+AVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY
Sbjct: 132 TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 191

Query: 234 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 293
           GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG
Sbjct: 192 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 251

Query: 294 RTRNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGT-- 353
           RTRNPWNIE+F+TGSSAGPAACTSAGMVPFAIGSET GSMTYPAARCGVTAIRPTFGT  
Sbjct: 252 RTRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG 311

Query: 354 ---DKLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYLDDADKE 413
              DKLGPFCRNAVDCAV+LDVIRGKDPHDLSSVES LDDPFSIDISKLTVGY+DDAD E
Sbjct: 312 RSGDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYVDDADME 371

Query: 414 VVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPT 473
           VV++LSSKGVNMIPFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPT
Sbjct: 372 VVQILSSKGVNMIPFNLSYSVDSVQGIVNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPT 431

Query: 474 ELRRARLISAIDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPVIVIP 533
           ELRRARLI A+DYVQAQRARGKLIRE+RESFNVDALIGNATDWEKVCMGNLVGLP+IV+P
Sbjct: 432 ELRRARLIPAVDYVQAQRARGKLIREIRESFNVDALIGNATDWEKVCMGNLVGLPIIVVP 491

Query: 534 TGFKNISNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPIDDLGP 593
           TGFKNISNPPST TTRR+  IT+GIYA PH+DHIALALAMAYQSATDHHRARPPIDDLGP
Sbjct: 492 TGFKNISNPPSTGTTRRKVTITSGIYAPPHRDHIALALAMAYQSATDHHRARPPIDDLGP 551

Query: 594 DDVIPDPPLVSIPPRLLHL 608
           +DV+PDPPLVSIPPRLLHL
Sbjct: 552 NDVLPDPPLVSIPPRLLHL 570

BLAST of HG10003229 vs. ExPASy Swiss-Prot
Match: Q3AD36 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=gatA PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.6e-40
Identity = 142/498 (28.51%), Postives = 223/498 (44.78%), Query Frame = 0

Query: 159 DIAFMSVLELGELIKTKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADEL 218
           ++ ++S  E+ E IK K+IS+ E+ +    R++  +  ++A V+ T EL  K A+E DE 
Sbjct: 2   ELYYLSAKEITEKIKAKEISAVEVAKATFDRIEAVEPKIQAYVTVTRELGLKMAREVDEK 61

Query: 219 FAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAK 278
            A+G   GPL G+P  +KD +S  G +TT  SK  +  +   +A V ++LK AGAV   K
Sbjct: 62  IARGEDPGPLAGVPVAIKDNMSTAGIRTTCSSKILENYIPPYDATVVEKLKEAGAVFTGK 121

Query: 279 LVTGSLAYDDIWFGGR---TRNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTY 338
                 A        R   TRNPW++E+   GSS G AA  +AG    A+GS+T GS+  
Sbjct: 122 TNLDEFAMGSSTENSRFFPTRNPWDLERVPGGSSGGSAASVAAGEAVVALGSDTGGSIRQ 181

Query: 339 PAARCGVTAIRPTFGT-------------DKLGPFCRNAVDCAVILDVIRGKDPHDLSSV 398
           PAA CG+  ++PT+G              D++GPF R   D A++L+VI G DP D +S 
Sbjct: 182 PAAFCGIVGLKPTYGAVSRYGLVAFASSLDQIGPFARTVEDAALLLNVIAGHDPKDSTSA 241

Query: 399 ESFLDDPFSI---DISKLTVG-----YLDDAD-------KEVVRVLSSKGVNMIPFNLSY 458
           +    D  S    DI  L +G     ++D  D        + ++VL S G      +L +
Sbjct: 242 DIEYPDYLSFLNQDIKGLKIGLPKEYFIDGIDAGVKKAIDDAIKVLESLGAVFEEVSLPH 301

Query: 459 SVDS--VQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQD-----------QWPTELRRAR 518
           +  S  V  ++        LA +D   R G  D +EA+D            +  E++R  
Sbjct: 302 TKYSLPVYYLIAPAEASSNLARYD-GVRYGYRD-FEAEDVVEMFSRTRAEGFGAEVKRRI 361

Query: 519 LISAID---------YVQAQRARGKLIREVRESF-NVDALIGNATDWEKVCMG------- 578
           ++             Y++A + R  +  +   +F  VD L+   T       G       
Sbjct: 362 MLGTYALSAGYYDAYYLKALKVRTLIKEDFDRAFTKVDLLLTPTTPTPAFKFGEKTSDPV 421

Query: 579 ------------NLVGLPVIVIPTGFKNISNPPSTATTRRRSAITTGIYAAPHKDHIALA 584
                       NL GLP I +P GF                 ++  +   P  +   L 
Sbjct: 422 SMYLSDIFTMAVNLAGLPGISVPAGFDG------------HLPVSFQLIGKPFDEGTLLK 481

BLAST of HG10003229 vs. ExPASy Swiss-Prot
Match: B2V855 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Sulfurihydrogenibium sp. (strain YO3AOP1) OX=436114 GN=gatA PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 8.5e-39
Identity = 137/494 (27.73%), Postives = 232/494 (46.96%), Query Frame = 0

Query: 164 SVLELGELIKTKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGV 223
           S+ EL +L+K+K++   E+V  F++R  + +  +KA V+  ++LA ++AK+ D+   +  
Sbjct: 7   SLKELSDLVKSKEVKPSEIVEAFIERKNQVEPKIKAYVTALDDLALEKAKKRDQELTKLE 66

Query: 224 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAK----- 283
            +  L G+P  +KD IS    KTT  SK  +  V   +A V ++LKS G V+  K     
Sbjct: 67  NIPDLFGLPIAIKDNISTKDIKTTCSSKMLENFVPVYDATVIERLKSQGYVITGKTNLDE 126

Query: 284 LVTGSLAYDDIWFGGRTRNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAA 343
              GS   +  +F   TRNPW++E+   GSS G AA  ++GM P ++GS+T GS+  PAA
Sbjct: 127 FAMGSSTENSAFF--PTRNPWDLERVPGGSSGGSAAVVASGMAPASLGSDTGGSIRQPAA 186

Query: 344 RCGVTAIRPTFG-------------TDKLGPFCRNAVDCAVILDVIRGKDPHDLSS---- 403
            CGV  ++PT+G              D++GPF R   D A+I++VI GKDP D +S    
Sbjct: 187 FCGVVGLKPTYGRVSRYGLVAFASSLDQIGPFGRTVEDVAMIMNVISGKDPKDSTSRSIP 246

Query: 404 VESFLDDPFSIDISKLTVG-----YLDDADKEV-------VRVLSSKGVNMIPFNLSYSV 463
           V ++L+   + D+  L +G     Y +D + ++       V+ L  +G+     +L Y+ 
Sbjct: 247 VPNYLES-LNKDVKGLKIGLPKEFYTEDLNPQIKEIILNAVKQLEKEGMTAHEISLPYTK 306

Query: 464 DSVQG--ILNFTMDVDMLAHFD---------EWQRLGLDDEYEAQDQWPTELRRARLISA 523
            +++   I+  +     LA FD         E++ L         + +  E++R  +I  
Sbjct: 307 YAIETYYIIAPSEASSNLARFDGVRYGYRAKEYKNLEEMYSKTRDEGFGAEVKRRIMIGT 366

Query: 524 ID---------YVQAQRARGKLIREVRESF-NVDALIGNATDWEKVCMG----------- 583
                      Y++AQ+ R  + ++   +F  VD +I   T      +G           
Sbjct: 367 YALSSGYYDAYYLKAQKVRTLIYQDYMNAFEKVDVIITPTTPDVAFKIGEKSNDPIQMYL 426

BLAST of HG10003229 vs. ExPASy Swiss-Prot
Match: P63497 (Putative amidase AmiD OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=amiD PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.7e-37
Identity = 132/467 (28.27%), Postives = 215/467 (46.04%), Query Frame = 0

Query: 156 NEEDIAFMSVLELGELIKTKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEA 215
           +E+ I+ + + E+ +LI+T+Q++S E+    L+R++R D  LK+      E A   A+ A
Sbjct: 13  DEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAA 72

Query: 216 DELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVL 275
           D   A+G Y G LHG+P G+KD+       T  G+  F++     +A V  +L++AGAV+
Sbjct: 73  DADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVI 132

Query: 276 VAKLVTGSLAYDDIWFGGRTR-NPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMT 335
           + KL     AY        T  NPW+   ++  SS+G    T+AG+   +IGS+T GS+ 
Sbjct: 133 IGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIR 192

Query: 336 YPAARCGVTAIRPTFGT-------------DKLGPFCRNAVDCAVILDVIRGKDPHDLS- 395
           +P + CGVT I+PT+G              D +GP  R+A D AV+L VI G D HD S 
Sbjct: 193 FPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDPSC 252

Query: 396 SVESFLDDPFSIDISKL---------TVGYLDDAD---KEVVRVLSSKGVNMIPFNLSYS 455
           S E   D    + ++++         T  + +D      +VV+ L   G  +I   L   
Sbjct: 253 SAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPAL 312

Query: 456 VDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLISAIDYVQAQRAR 515
              V            +AH D +      DEY    +    +     ++A++Y      R
Sbjct: 313 APMVAAFGKMRAVETAIAHADTYPARA--DEYGPIMR--AMIDAGHRLAAVEYQTLTERR 372

Query: 516 GKLIREVRESF-NVDALIGNATDWEKVCMGNLVGL-------PVIVIPTGFKNISNPPS- 575
            +  R +R  F +VD L+  +       +  + GL         + +PT   N+S  P+ 
Sbjct: 373 LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAI 432

Query: 576 ---TATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPI 584
                TT R + +          +H+ +    A+Q  T +HR RPP+
Sbjct: 433 CLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV 475

BLAST of HG10003229 vs. ExPASy Swiss-Prot
Match: P9WQ92 (Putative amidase AmiD OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=amiD PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.7e-37
Identity = 132/467 (28.27%), Postives = 215/467 (46.04%), Query Frame = 0

Query: 156 NEEDIAFMSVLELGELIKTKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEA 215
           +E+ I+ + + E+ +LI+T+Q++S E+    L+R++R D  LK+      E A   A+ A
Sbjct: 13  DEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAA 72

Query: 216 DELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVL 275
           D   A+G Y G LHG+P G+KD+       T  G+  F++     +A V  +L++AGAV+
Sbjct: 73  DADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVI 132

Query: 276 VAKLVTGSLAYDDIWFGGRTR-NPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMT 335
           + KL     AY        T  NPW+   ++  SS+G    T+AG+   +IGS+T GS+ 
Sbjct: 133 IGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIR 192

Query: 336 YPAARCGVTAIRPTFGT-------------DKLGPFCRNAVDCAVILDVIRGKDPHDLS- 395
           +P + CGVT I+PT+G              D +GP  R+A D AV+L VI G D HD S 
Sbjct: 193 FPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDPSC 252

Query: 396 SVESFLDDPFSIDISKL---------TVGYLDDAD---KEVVRVLSSKGVNMIPFNLSYS 455
           S E   D    + ++++         T  + +D      +VV+ L   G  +I   L   
Sbjct: 253 SAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPAL 312

Query: 456 VDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLISAIDYVQAQRAR 515
              V            +AH D +      DEY    +    +     ++A++Y      R
Sbjct: 313 APMVAAFGKMRAVETAIAHADTYPARA--DEYGPIMR--AMIDAGHRLAAVEYQTLTERR 372

Query: 516 GKLIREVRESF-NVDALIGNATDWEKVCMGNLVGL-------PVIVIPTGFKNISNPPS- 575
            +  R +R  F +VD L+  +       +  + GL         + +PT   N+S  P+ 
Sbjct: 373 LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAI 432

Query: 576 ---TATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPI 584
                TT R + +          +H+ +    A+Q  T +HR RPP+
Sbjct: 433 CLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV 475

BLAST of HG10003229 vs. ExPASy Swiss-Prot
Match: P9WQ93 (Putative amidase AmiD OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=amiD PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.7e-37
Identity = 132/467 (28.27%), Postives = 215/467 (46.04%), Query Frame = 0

Query: 156 NEEDIAFMSVLELGELIKTKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEA 215
           +E+ I+ + + E+ +LI+T+Q++S E+    L+R++R D  LK+      E A   A+ A
Sbjct: 13  DEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAA 72

Query: 216 DELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVL 275
           D   A+G Y G LHG+P G+KD+       T  G+  F++     +A V  +L++AGAV+
Sbjct: 73  DADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVI 132

Query: 276 VAKLVTGSLAYDDIWFGGRTR-NPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMT 335
           + KL     AY        T  NPW+   ++  SS+G    T+AG+   +IGS+T GS+ 
Sbjct: 133 IGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIR 192

Query: 336 YPAARCGVTAIRPTFGT-------------DKLGPFCRNAVDCAVILDVIRGKDPHDLS- 395
           +P + CGVT I+PT+G              D +GP  R+A D AV+L VI G D HD S 
Sbjct: 193 FPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDPSC 252

Query: 396 SVESFLDDPFSIDISKL---------TVGYLDDAD---KEVVRVLSSKGVNMIPFNLSYS 455
           S E   D    + ++++         T  + +D      +VV+ L   G  +I   L   
Sbjct: 253 SAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPAL 312

Query: 456 VDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLISAIDYVQAQRAR 515
              V            +AH D +      DEY    +    +     ++A++Y      R
Sbjct: 313 APMVAAFGKMRAVETAIAHADTYPARA--DEYGPIMR--AMIDAGHRLAAVEYQTLTERR 372

Query: 516 GKLIREVRESF-NVDALIGNATDWEKVCMGNLVGL-------PVIVIPTGFKNISNPPS- 575
            +  R +R  F +VD L+  +       +  + GL         + +PT   N+S  P+ 
Sbjct: 373 LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAI 432

Query: 576 ---TATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPI 584
                TT R + +          +H+ +    A+Q  T +HR RPP+
Sbjct: 433 CLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV 475

BLAST of HG10003229 vs. ExPASy TrEMBL
Match: A0A0A0L5U7 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G039330 PE=4 SV=1)

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 546/620 (88.06%), Postives = 573/620 (92.42%), Query Frame = 0

Query: 2   PIDKCPPMALTPTVIAANCF-FFFFFVFFAVPVRSSISPDFSSFHQCSCPRFTPMDSKKD 61
           P DKCP MA TPT+   NCF  FFFF FF V VRSSIS  FSSF QCS  RFT MDSKKD
Sbjct: 68  PFDKCPTMASTPTI---NCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMDSKKD 127

Query: 62  NECLENVGKTSLLKCAFGAFDADFFNNTKMDEVLKGAKEFNLPTIRANQKLVASENGGLH 121
           +ECLEN+G+TS+LKC F A DADFFNNTK DEVLKGA+E N+P IRAN+KLVASENGGLH
Sbjct: 128 SECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLH 187

Query: 122 NPSPLVFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMSVLELGELIKTKQISSQ 181
           NPS LVFNPEWANE  RHKSKRFCYPPVSG+KRPNNE+DIAFMSVLELGELIKT+QISSQ
Sbjct: 188 NPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQ 247

Query: 182 ELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIIS 241
           ELVRIFLQRLKRY+HVL+AVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIIS
Sbjct: 248 ELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIIS 307

Query: 242 VPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWN 301
           VPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWN
Sbjct: 308 VPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWN 367

Query: 302 IEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGT--------- 361
           IE+FSTGSSAGPAACTSAGMVPFAIGSET GSMTYPAARCGVTAIRPTFGT         
Sbjct: 368 IEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTAIRPTFGTVGRSGVMSI 427

Query: 362 ----DKLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYLDDADK 421
               DKLGPFCRNAVDCAV+LDVIRGKDPHDLSSVES LDDPFSIDISKLTVGYLDDAD 
Sbjct: 428 SESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADM 487

Query: 422 EVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWP 481
           EVVR+LSSKGVNM+PFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRLGLDDEYEAQDQWP
Sbjct: 488 EVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWP 547

Query: 482 TELRRARLISAIDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPVIVI 541
           TELRRARLI A+DYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLP++V+
Sbjct: 548 TELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPIVVV 607

Query: 542 PTGFKNISNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPIDDLG 601
           PTGFKNISNPPST TTRR++ ITTGIYA PH+DHIALALAMAYQSATDHHRA+PPIDDLG
Sbjct: 608 PTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPIDDLG 667

Query: 602 PDDVIPDPPLVSIPPRLLHL 608
           P DV+PDPPLVSIPPRLLHL
Sbjct: 668 PSDVLPDPPLVSIPPRLLHL 684

BLAST of HG10003229 vs. ExPASy TrEMBL
Match: A0A5D3D785 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00780 PE=4 SV=1)

HSP 1 Score: 1042.3 bits (2694), Expect = 7.7e-301
Identity = 510/559 (91.23%), Postives = 537/559 (96.06%), Query Frame = 0

Query: 54  PMDSKKDNECLENVGKTSLLKCAFGAFDADFFNNTKMDEVLKGAKEFNLPTIRANQKLVA 113
           P  SKKD+ECLEN+G+TS+LKC F AFDADFFNNTK DEVLKGA+E N+PTIRAN+KLVA
Sbjct: 12  PFCSKKDSECLENMGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVA 71

Query: 114 SENGGLHNPSPLVFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMSVLELGELIK 173
           SENGGLHNPSPLVFNPEWANE  RHKSKRFCYPPV GVKRPNNE+DIAFMSVLELGELIK
Sbjct: 72  SENGGLHNPSPLVFNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIK 131

Query: 174 TKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 233
           T+QISSQELVRIFLQRLKRY+HVL+AVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY
Sbjct: 132 TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 191

Query: 234 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 293
           GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG
Sbjct: 192 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 251

Query: 294 RTRNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGT-- 353
           RTRNPWNIE+F+TGSSAGPAACTSAGMVPFAIGSET GSMTYPAARCGVTAIRPTFGT  
Sbjct: 252 RTRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG 311

Query: 354 ---DKLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYLDDADKE 413
              DKLGPFCRNAVDCAV+LDVIRGKDPHDLSSVES LDDPFSIDISKLTVGY+DDAD E
Sbjct: 312 RSGDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYVDDADME 371

Query: 414 VVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPT 473
           VV++LSSKGVNMIPFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPT
Sbjct: 372 VVQILSSKGVNMIPFNLSYSVDSVQGIVNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPT 431

Query: 474 ELRRARLISAIDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPVIVIP 533
           ELRRARLI A+DYVQAQRARGKLIRE+RESFNVDALIGNATDWEKVCMGNLVGLP+IV+P
Sbjct: 432 ELRRARLIPAVDYVQAQRARGKLIREIRESFNVDALIGNATDWEKVCMGNLVGLPIIVVP 491

Query: 534 TGFKNISNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPIDDLGP 593
           TGFKNISNPPST TTRR+  IT+GIYA PH+DHIALALAMAYQSATDHHRARPPIDDLGP
Sbjct: 492 TGFKNISNPPSTGTTRRKVTITSGIYAPPHRDHIALALAMAYQSATDHHRARPPIDDLGP 551

Query: 594 DDVIPDPPLVSIPPRLLHL 608
           +DV+PDPPLVSIPPRLLHL
Sbjct: 552 NDVLPDPPLVSIPPRLLHL 570

BLAST of HG10003229 vs. ExPASy TrEMBL
Match: A0A1S4DX80 (glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo OX=3656 GN=LOC103490981 PE=4 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 3.8e-300
Identity = 510/565 (90.27%), Postives = 537/565 (95.04%), Query Frame = 0

Query: 56  DSKKDNECLENVGKTSLLKCAFGAFDADFFNNTKMDEVLKGAKEFNLPTIRANQKLVASE 115
           DSKKD+ECLEN+G+TS+LKC F AFDADFFNNTK DEVLKGA+E N+PTIRAN+KLVASE
Sbjct: 27  DSKKDSECLENMGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVASE 86

Query: 116 NGGLHNPSPLVFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMSVLELGELIKTK 175
           NGGLHNPSPLVFNPEWANE  RHKSKRFCYPPV GVKRPNNE+DIAFMSVLELGELIKT+
Sbjct: 87  NGGLHNPSPLVFNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIKTE 146

Query: 176 QISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGL 235
           QISSQELVRIFLQRLKRY+HVL+AVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGL
Sbjct: 147 QISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGL 206

Query: 236 KDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRT 295
           KDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRT
Sbjct: 207 KDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRT 266

Query: 296 RNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGT---- 355
           RNPWNIE+F+TGSSAGPAACTSAGMVPFAIGSET GSMTYPAARCGVTAIRPTFGT    
Sbjct: 267 RNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRS 326

Query: 356 ---------DKLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYL 415
                    DKLGPFCRNAVDCAV+LDVIRGKDPHDLSSVES LDDPFSIDISKLTVGY+
Sbjct: 327 GVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYV 386

Query: 416 DDADKEVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEA 475
           DDAD EVV++LSSKGVNMIPFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRLGLDDEYEA
Sbjct: 387 DDADMEVVQILSSKGVNMIPFNLSYSVDSVQGIVNFTMDVDMLAHFDEWQRLGLDDEYEA 446

Query: 476 QDQWPTELRRARLISAIDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGL 535
           QDQWPTELRRARLI A+DYVQAQRARGKLIRE+RESFNVDALIGNATDWEKVCMGNLVGL
Sbjct: 447 QDQWPTELRRARLIPAVDYVQAQRARGKLIREIRESFNVDALIGNATDWEKVCMGNLVGL 506

Query: 536 PVIVIPTGFKNISNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPP 595
           P+IV+PTGFKNISNPPST TTRR+  IT+GIYA PH+DHIALALAMAYQSATDHHRARPP
Sbjct: 507 PIIVVPTGFKNISNPPSTGTTRRKVTITSGIYAPPHRDHIALALAMAYQSATDHHRARPP 566

Query: 596 IDDLGPDDVIPDPPLVSIPPRLLHL 608
           IDDLGP+DV+PDPPLVSIPPRLLHL
Sbjct: 567 IDDLGPNDVLPDPPLVSIPPRLLHL 591

BLAST of HG10003229 vs. ExPASy TrEMBL
Match: A0A6J1KX47 (uncharacterized protein LOC111498360 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498360 PE=4 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.4e-297
Identity = 520/622 (83.60%), Postives = 558/622 (89.71%), Query Frame = 0

Query: 1   MPIDKCPPMALTPTVIAANCFFFFFFV--FFAVPVRSSISPDFSSFHQCSCPRFTPMDSK 60
           MPIDK   M  T T IA+NCFFF FFV  FFAVP+RSSISP+FSS  Q S P FT MD  
Sbjct: 1   MPIDKWLSMPATRTAIASNCFFFVFFVFIFFAVPLRSSISPNFSSSDQ-SRPCFTAMDRN 60

Query: 61  KDNECLENVGKTSLLKCAFGAFDADFFNNTKMDEVLKGAKEFNLPTIRANQKLVASENGG 120
           KD E  +N GK S     F AFDA+FF+ T+MDEVLKGA+EFN P IRAN+KLVASENGG
Sbjct: 61  KDTESFKNGGKRSF----FRAFDAEFFSKTEMDEVLKGAEEFNFPVIRANRKLVASENGG 120

Query: 121 LHNPSPLVFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMSVLELGELIKTKQIS 180
           LHNPSPLVF+  WANE F+HKS+RFCYPPV GVKRP NEEDIAFMSVLELGELIKTKQI+
Sbjct: 121 LHNPSPLVFSSNWANEGFQHKSRRFCYPPVFGVKRPKNEEDIAFMSVLELGELIKTKQIT 180

Query: 181 SQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDI 240
           SQELV IFLQRLKRYD+VL+AVVSFTE+LAYKQAKEADELFA+GVYLGPLHGIPYGLKDI
Sbjct: 181 SQELVGIFLQRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGVYLGPLHGIPYGLKDI 240

Query: 241 ISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNP 300
           ISVPGYKTTWGSKSFKEQVIDVEAWVY+QLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNP
Sbjct: 241 ISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNP 300

Query: 301 WNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGT------- 360
           WNIE+F+TGSSAGPAACTSAG+VPFAIGSETAGSMTYPA+RCGVTA+RPTFGT       
Sbjct: 301 WNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTYPASRCGVTALRPTFGTVGRSGVM 360

Query: 361 ------DKLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYLDDA 420
                 DKLGPFCRNA+DCAVILDVIRGKDPHDLSSVES LDDPFSIDISKLTVGYLDDA
Sbjct: 361 SISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDA 420

Query: 421 DKEVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQ 480
           D EVVRVLSSKG NM+PFNL+YS+DSVQGILNFTMD+DMLAHFDEWQR GLD EYEAQDQ
Sbjct: 421 DMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLAHFDEWQRSGLDHEYEAQDQ 480

Query: 481 WPTELRRARLISAIDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPVI 540
           WPTELRRARLI A+DY+QAQRARGKLIREV+ESFNVDALIGNATDWE+VCMGNLVGLPV+
Sbjct: 481 WPTELRRARLIPAVDYLQAQRARGKLIREVKESFNVDALIGNATDWERVCMGNLVGLPVM 540

Query: 541 VIPTGFKNISNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPIDD 600
           V+PTGFKNISNPP+T TTRRR+AITTGIYA PHKDHIALALAMAYQSATDHHRARPPIDD
Sbjct: 541 VVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAYQSATDHHRARPPIDD 600

Query: 601 LGPDDVIPDPPLVSIPPRLLHL 608
           LGP D IP+PP V IPPRLL +
Sbjct: 601 LGPHDAIPEPPSVPIPPRLLRV 617

BLAST of HG10003229 vs. ExPASy TrEMBL
Match: A0A6J1G4E4 (uncharacterized protein LOC111450695 OS=Cucurbita moschata OX=3662 GN=LOC111450695 PE=4 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 9.7e-296
Identity = 522/645 (80.93%), Postives = 560/645 (86.82%), Query Frame = 0

Query: 1   MPIDKCPPMALTPTVIAANCFFFFFFV--FFAVPVRSSISPDFSSFHQCSCPRFTPMDS- 60
           MPIDK   M  T T +A+NCFFF FFV  FFAVP+RSSISP+FSS  Q S P FT M + 
Sbjct: 1   MPIDKWLSMPATRTTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQ-SRPCFTAMGAT 60

Query: 61  ----------------------KKDNECLENVGKTSLLKCAFGAFDADFFNNTKMDEVLK 120
                                  KD E  +N GK S     F AFDADFF+NT+MDEVLK
Sbjct: 61  YKKIWKLLCEKVGVHYRSPSYRNKDTESFKNGGKRSF----FRAFDADFFSNTEMDEVLK 120

Query: 121 GAKEFNLPTIRANQKLVASENGGLHNPSPLVFNPEWANEKFRHKSKRFCYPPVSGVKRPN 180
           GA+E NLP IRAN+KLVASENGGLHNPSPLVF+  WANE+F HKS+RFCYPPVSGVKRP 
Sbjct: 121 GAEELNLPVIRANRKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPK 180

Query: 181 NEEDIAFMSVLELGELIKTKQISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEA 240
           NEEDIAFMSVLELGELIKTKQI+SQELV IFL+RLKRYD+VL+AVVSFTE+LAYKQAKEA
Sbjct: 181 NEEDIAFMSVLELGELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEA 240

Query: 241 DELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVL 300
           DELFA+GVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVY+QLKSAGAVL
Sbjct: 241 DELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVL 300

Query: 301 VAKLVTGSLAYDDIWFGGRTRNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTY 360
           V KLVTGSLAYDDIWFGGRTRNPWNIE+F+TGSSAGPAACTSAG+VPFAIGSETAGSMTY
Sbjct: 301 VTKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTY 360

Query: 361 PAARCGVTAIRPTFGT-------------DKLGPFCRNAVDCAVILDVIRGKDPHDLSSV 420
           PA+RCGVTA+RPTFGT             DKLGPFCRNA+DCAVILDVIRGKDPHDLSSV
Sbjct: 361 PASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSV 420

Query: 421 ESFLDDPFSIDISKLTVGYLDDADKEVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDV 480
           ESFLDDPFSIDISKLTVGYLDDAD EVVRVLSSKG NM+PFNL+YS+DSVQGILNFTMD+
Sbjct: 421 ESFLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDI 480

Query: 481 DMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLISAIDYVQAQRARGKLIREVRESFNVD 540
           DML HFDEWQR GLD EYEA+DQWPTELRRARLI A+DYVQAQRARGKL REV+ESFNVD
Sbjct: 481 DMLTHFDEWQRSGLDHEYEAEDQWPTELRRARLIPAVDYVQAQRARGKLRREVKESFNVD 540

Query: 541 ALIGNATDWEKVCMGNLVGLPVIVIPTGFKNISNPPSTATTRRRSAITTGIYAAPHKDHI 600
           ALIGNATDWE+VCMGNLVGLPVIV+PTGFKNISNPP+T TTRRR+AITTGIYA PHKDHI
Sbjct: 541 ALIGNATDWERVCMGNLVGLPVIVVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHI 600

Query: 601 ALALAMAYQSATDHHRARPPIDDLGPDDVIPDPPLVSIPPRLLHL 608
           ALALAMAYQSATDHHRARPPIDDLGP D IP+PPLV IPPRLLHL
Sbjct: 601 ALALAMAYQSATDHHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 640

BLAST of HG10003229 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 834.3 bits (2154), Expect = 6.1e-242
Identity = 410/555 (73.87%), Postives = 468/555 (84.32%), Query Frame = 0

Query: 69  KTSLLKCAFGAF---DADFFNNTKMDEVLKGAKEFNLPTIRANQKLVASENGGLHNPSPL 128
           KT   KC  G F   D+ FFN TK+ E+ KGA E N+P  RAN+KLVA++NGGL NPSPL
Sbjct: 106 KTMEAKCLQGMFELIDSAFFNETKLQEIAKGATEMNVPIYRANRKLVATKNGGLENPSPL 165

Query: 129 VFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMSVLELGELIKTKQISSQELVRI 188
           VFNP W  E  R + KRF YP  SGVK P +EEDIAFMSVLELGELIKT+QI+S+ELVRI
Sbjct: 166 VFNPSWNREVQRVQDKRFKYPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRI 225

Query: 189 FLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYK 248
           +L++LKRY+HVL+AVV++TEELAYKQAKEAD+L +QG YLGPLHGIPYGLKDI++VPGYK
Sbjct: 226 YLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPGYK 285

Query: 249 TTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEQFS 308
           TTWGS SFK+Q +D+EAWVYK+LK++GAVLVAKLVTGS+AYDDIWFGGRTRNPWNIE+FS
Sbjct: 286 TTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWFGGRTRNPWNIEEFS 345

Query: 309 TGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGT-------------D 368
           TGSSAGPAA TSAGMVPFAIGSETAGSMTYPAARCG+TA+RPTFG+             D
Sbjct: 346 TGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLD 405

Query: 369 KLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYLDDADKEVVRV 428
           KLGPFCR A DCAVILD I+GKDP DLSS E   +DPFS+DI+KLTVGY  DAD +VV V
Sbjct: 406 KLGPFCRTAADCAVILDAIKGKDPDDLSSREIAFEDPFSVDITKLTVGYTKDADMKVVEV 465

Query: 429 LSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRR 488
           L SKGVNM+PF L+Y+V+SVQGILNFTMDVDMLAHFDEWQR G +D YEAQDQWP ELRR
Sbjct: 466 LGSKGVNMVPFELNYTVESVQGILNFTMDVDMLAHFDEWQRTGQEDLYEAQDQWPVELRR 525

Query: 489 ARLISAIDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPVIVIPTGFK 548
           AR+++A+DY+QAQRARGKLIREV +SF VDA IGN TDWEKVCMGNLVGLPV+VIPTGFK
Sbjct: 526 ARVVTAVDYIQAQRARGKLIREVEKSFTVDAFIGNVTDWEKVCMGNLVGLPVLVIPTGFK 585

Query: 549 NISNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPIDDLGPDDVI 608
           NI++PP T + RRR+ I  GIYA P +DHIALAL MAYQS TD HR RPPIDDLGPDD I
Sbjct: 586 NITDPP-TNSCRRRTTINAGIYAPPERDHIALALGMAYQSVTDAHRKRPPIDDLGPDDSI 645

BLAST of HG10003229 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 813.9 bits (2101), Expect = 8.5e-236
Identity = 403/555 (72.61%), Postives = 461/555 (83.06%), Query Frame = 0

Query: 69  KTSLLKCAFGAF---DADFFNNTKMDEVLKGAKEFNLPTIRANQKLVASENGGLHNPSPL 128
           KT   KC  G F   D+ FFN TK+ E+ KGA E N+P  RAN+KLVA++NGGL NPSPL
Sbjct: 106 KTMEAKCLQGMFELIDSAFFNETKLQEIAKGATEMNVPIYRANRKLVATKNGGLENPSPL 165

Query: 129 VFNPEWANEKFRHKSKRFCYPPVSGVKRPNNEEDIAFMSVLELGELIKTKQISSQELVRI 188
           VFNP W  E  R + KRF YP  SGVK P +EEDIAFMSVLELGELIKT+QI+S+ELVRI
Sbjct: 166 VFNPSWNREVQRVQDKRFKYPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRI 225

Query: 189 FLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYK 248
           +L++LKRY+HVL+AVV++TEELAYKQAKEAD+L +QG YLGPLHGIPYGLKDI++VPGYK
Sbjct: 226 YLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPGYK 285

Query: 249 TTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEQFS 308
           TTWGS SFK+Q +D+EAWVYK+LK++GAVLVAKLVTGS+AYDDIWFGGRTRNPWNIE+FS
Sbjct: 286 TTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWFGGRTRNPWNIEEFS 345

Query: 309 TGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRPTFGT-------------D 368
           TGSSAGPAA TSA       GSETAGSMTYPAARCG+TA+RPTFG+             D
Sbjct: 346 TGSSAGPAASTSA-------GSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLD 405

Query: 369 KLGPFCRNAVDCAVILDVIRGKDPHDLSSVESFLDDPFSIDISKLTVGYLDDADKEVVRV 428
           KLGPFCR A DCAVILD I+GKDP DLSS E   +DPFS+DI+KLTVGY  DAD +VV V
Sbjct: 406 KLGPFCRTAADCAVILDAIKGKDPDDLSSREIAFEDPFSVDITKLTVGYTKDADMKVVEV 465

Query: 429 LSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRR 488
           L SKGVNM+PF L+Y+V+SVQGILNFTMDVDMLAHFDEWQR G +D YEAQDQWP ELRR
Sbjct: 466 LGSKGVNMVPFELNYTVESVQGILNFTMDVDMLAHFDEWQRTGQEDLYEAQDQWPVELRR 525

Query: 489 ARLISAIDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPVIVIPTGFK 548
           AR+++A+DY+QAQRARGKLIREV +SF VDA IGN TDWEKVCMGNLVGLPV+VIPTGFK
Sbjct: 526 ARVVTAVDYIQAQRARGKLIREVEKSFTVDAFIGNVTDWEKVCMGNLVGLPVLVIPTGFK 585

Query: 549 NISNPPSTATTRRRSAITTGIYAAPHKDHIALALAMAYQSATDHHRARPPIDDLGPDDVI 608
           NI++PP T + RRR+ I  GIYA P +DHIALAL MAYQS TD HR RPPIDDLGPDD I
Sbjct: 586 NITDPP-TNSCRRRTTINAGIYAPPERDHIALALGMAYQSVTDAHRKRPPIDDLGPDDSI 645

BLAST of HG10003229 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 99.8 bits (247), Expect = 8.2e-21
Identity = 70/243 (28.81%), Postives = 113/243 (46.50%), Query Frame = 0

Query: 176 QISSQELVRIFLQRLKRYDHVLKAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGL 235
           + ++ E+ + +L R++  +  LK  +  +E +  K A+E D+  A+G  LGPL G+  G+
Sbjct: 57  ETTAVEIAKSYLSRIRLTEPQLKCFLHVSENV-LKDAQEIDQRIAKGEELGPLAGVLIGV 116

Query: 236 KDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRT 295
           KD I   G  +T  S+  +      +A   K++K  G ++V     G    D+   G  T
Sbjct: 117 KDNICTQGMPSTAASRILEHYRPPFDATAVKKIKELGGIVV-----GKTNMDEFGMGSTT 176

Query: 296 R--------NPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRP 355
                    NPW++ +   GSS G AA  +A     ++GS+T GS+  PA+ CGV  ++P
Sbjct: 177 EASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKP 236

Query: 356 TFG-------------TDKLGPFCRNAVDCAVILDVIRGKDPHDLSS----VESFLDDPF 394
           T+G              D +G F     D  ++L  I G D  D +S    V  F     
Sbjct: 237 TYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQDVPEFQSQFL 293

BLAST of HG10003229 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 65.1 bits (157), Expect = 2.2e-10
Identity = 41/128 (32.03%), Postives = 63/128 (49.22%), Query Frame = 0

Query: 228 LHGIPYGLKDIISVPGYKTTWGSKSF--KEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLA 287
           L G+ + +KDI  V G  T +G+  +          A V   L  AGA  +   +   +A
Sbjct: 28  LQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPVVSSLLEAGATALGITIMDEMA 87

Query: 288 YD---DIWFGGRTRNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGV 347
           Y    +    G  RNP   ++   GSS+G A   +A +V F+IG++T GS+  PA+ CG+
Sbjct: 88  YSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLVDFSIGTDTGGSVRVPASYCGI 147

Query: 348 TAIRPTFG 351
              RP+ G
Sbjct: 148 FGFRPSHG 155

BLAST of HG10003229 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 57.4 bits (137), Expect = 4.7e-08
Identity = 39/129 (30.23%), Postives = 58/129 (44.96%), Query Frame = 0

Query: 228 LHGIPYGLKDIISVPGYKTTWGSKSFKE--QVIDVEAWVYKQLKSAGAVLVAKLVTGSLA 287
           L G+ + + D   V  Y T +G   +K+  +  +  A V   L   GA  V K +   L 
Sbjct: 71  LSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAEKTAVVVTTLLKNGATCVGKTIMDELG 130

Query: 288 YDDIWFG---GRTRNPWNIEQFSTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGV 347
           +  I      G   NP   +    G S+G A    A +V F++G +T G +  PAA CG+
Sbjct: 131 FGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSVGAELVDFSLGIDTTGGVRVPAAFCGI 190

Query: 348 TAIRPTFGT 352
              RP+ GT
Sbjct: 191 LGFRPSQGT 199