HG10003189 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10003189 vs. NCBI nr
Match: XP_016903409.1 (PREDICTED: putative disease resistance protein RGA1 [Cucumis melo])

HSP 1 Score: 350.5 bits (898), Expect = 2.5e-92
Identity = 198/369 (53.66%), Postives = 236/369 (63.96%), Query Frame = 0

Query: 180 LQNSNLVQIGRHNSGELNELNGLNKLRGSLSIQNLQFCTS-ADLQMEAKFLQLKSGLQTL 239
           LQ  N   +G++   EL EL+GL KLRG LSI++L FC+S  D QM+ KFLQ KS LQ L
Sbjct: 301 LQTMNTFVLGKNKGSELKELDGLTKLRGELSIRHLAFCSSIVDQQMKGKFLQQKSDLQVL 360

Query: 240 DLQWDD--LFDHEPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGC 299
           DL W+   + D E    + D   E V+DCLQPHSNL++I+I  Y GVKLCNW+SS  LGC
Sbjct: 361 DLNWEKHLIVDDE----LEDVIYESVLDCLQPHSNLRKIYINGYGGVKLCNWVSSKFLGC 420

Query: 300 LIKIRLDGCERLQHLPRLDQFPYLKYLYLRDLPNIEYI--GNDDSVPSSTIFPSFEELR- 359
           L+ +RL  CERL+HLPR DQFP LK L L  LPNIEYI   N+DSV SST FPS +EL  
Sbjct: 421 LVTVRLYHCERLRHLPRFDQFPNLKRLSLEVLPNIEYIIVNNNDSVSSSTNFPSMKELEI 480

Query: 360 ----------------------FPCLSVLEIHG-CPLHILMSWHAP-LKSLTIGNSRNGG 419
                                 FPCLS L I+G CPLH+L  WHAP LKSL I +S    
Sbjct: 481 SNMPKLVSWCKGTTLAKSPIIIFPCLSSLTINGRCPLHMLKFWHAPNLKSLKISDSE--- 540

Query: 420 HCLKGDEFDVVPWKIFENLSSLGLFGMRKLEYLPECLQH-VTTLQSLTLWFCQNLKCLPR 479
                DE +VVP K +ENL+SL LF + +LEYLPEC QH +T+L+ L L  C+NLK LP 
Sbjct: 541 -----DELNVVPLKNYENLTSLCLFNLSELEYLPECWQHCMTSLRFLCLGRCKNLKSLPE 600

Query: 480 WIGNLTSLTNFEIVCCEKLTLLPEEICHIHGLVSLVINGCPELEEKCKKGTGEDWPKIAH 518
           WI N TSL   +I  CE LTLLPE I HI  L  L I  CP LEE+CK+  GEDW KIAH
Sbjct: 601 WIDNFTSLDRLDIFSCENLTLLPEGIQHIRNLQRLRIECCPVLEERCKENIGEDWHKIAH 657

BLAST of HG10003189 vs. NCBI nr
Match: XP_022154678.1 (putative disease resistance protein RGA4 [Momordica charantia])

HSP 1 Score: 327.4 bits (838), Expect = 2.2e-85
Identity = 190/382 (49.74%), Postives = 226/382 (59.16%), Query Frame = 0

Query: 191  HNSGELNELNGLNKLRGSLSIQNLQFCTSADLQMEAKFLQLKSGLQ--TLDLQWDDLFDH 250
            +N G L+ELN LNKLRGSL+I  L FCT++DL   AKFL LKS LQ   L+ +WD+   +
Sbjct: 788  NNGGGLSELNELNKLRGSLTINRLNFCTTSDLPTNAKFLLLKSSLQELVLEWEWDNDSVN 847

Query: 251  EPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGCLIKIRLDGCERL 310
            EPT    DDDCE V++CL PHSNL+ + I +Y+GVKLCNW+SS+ LGCL+ I+L GC+RL
Sbjct: 848  EPTIGDLDDDCESVLECLHPHSNLRNLCIDKYRGVKLCNWVSSNFLGCLVTIKLSGCKRL 907

Query: 311  QHLPRLDQFPYLKYLYLRDLPNIEYIGNDDSVPSSTIFPSFEELR--------------- 370
            QHLP+ DQFP LK L LR+L  IEYI NDD   SST+FPS EEL                
Sbjct: 908  QHLPQFDQFPNLKRLSLRELSGIEYIFNDDYRCSSTLFPSLEELSIFCMPNLKRWWKKGQ 967

Query: 371  ---------------FPCLSVLEIHGCPLHILMSWHAPLKSLTIGNSRNGGHCLKGDEFD 430
                           FPCLS LEI GCPL   ++ HAP KSL + +          DE D
Sbjct: 968  TSKATSSQNVLFPIIFPCLSKLEIWGCPLLASITCHAPPKSLEMNDPIE--FHFDPDESD 1027

Query: 431  VVPWKIFENLSSLGLFGMRKLEYLPECLQHVTTLQSLTLWFCQN---------------- 490
            VV WK FE+LS L L  + +  +L   LQHVTTLQ LTL +CQN                
Sbjct: 1028 VVSWKCFESLSYLRLTHIDEWGFLSNWLQHVTTLQELTLEYCQNLTNLPESISNFTSLSK 1087

Query: 491  ---LKC-----LPRWIGNLTSLTNFEIVCCEKLTLLPEEICHIHGLVSLVINGCPELEEK 517
               LKC     LP  IGNLTSLT   I  C  LT LPE I H+H L SL    CP LE+ 
Sbjct: 1088 LEILKCTDLMSLPESIGNLTSLTELTIDFCPSLTSLPEGIRHLHCLQSLTTWDCPILEKM 1147

BLAST of HG10003189 vs. NCBI nr
Match: XP_022154678.1 (putative disease resistance protein RGA4 [Momordica charantia])

HSP 1 Score: 213.4 bits (542), Expect = 4.7e-51
Identity = 121/189 (64.02%), Postives = 141/189 (74.60%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESR 60
           MA+SILFN AGT++ KLG +   EL LL GV  EL+KLK TV+AI  VLLDAE++ S+S 
Sbjct: 1   MADSILFNLAGTLINKLGXYVHQELGLLLGVNGELDKLKGTVSAIXKVLLDAEERSSKSH 60

Query: 61  AVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKM 120
           A++DW+SKLK  FY++DDLIDEF+YE LRRQVMT   RK  KQVRIFFSKSNQIAF  KM
Sbjct: 61  ALEDWLSKLKGVFYEIDDLIDEFNYEALRRQVMTRNRRK-TKQVRIFFSKSNQIAFSHKM 120

Query: 121 GHKIKEIRESLKAINDDKTQFSFSECMIDRR-YDGFRERRETGSYIPEEEVIGLERPKEE 180
           G KIKEIRE LK INDDKTQF  SEC+I++   D  R  R+ G YIPEEEVIG    K+E
Sbjct: 121 GGKIKEIREKLKVINDDKTQFKLSECVINKSDVDILRNTRDPGDYIPEEEVIGRNYEKKE 180

Query: 181 ----LQNSN 185
               L NSN
Sbjct: 181 IIDLLLNSN 188

HSP 2 Score: 321.2 bits (822), Expect = 1.6e-83
Identity = 196/388 (50.52%), Postives = 233/388 (60.05%), Query Frame = 0

Query: 180  LQNSNLVQIGRHNSGELNELNGLNKLRGSLSIQNLQFCTS-ADLQMEAKFLQLKSGLQTL 239
            LQ      +G++  GEL EL GL KLRG LSI++L+ CTS  D QM++K LQLKSGLQ L
Sbjct: 730  LQTLTTFVLGKNIGGELKELEGLTKLRGGLSIKHLESCTSIVDQQMKSKLLQLKSGLQKL 789

Query: 240  DLQWDDLFDHEPTTDISDDDCERVM-----DCLQPHSNLQEIFIRRYKGVKLCNWLSSD- 299
            +LQW           I DD  E VM     DCLQPHSNL+EI I  Y GV LCNW+SS+ 
Sbjct: 790  ELQW-------KKPKIGDDQLEDVMYESVLDCLQPHSNLKEIRIDGYGGVNLCNWVSSNK 849

Query: 300  SLGCLIKIRLDGCERLQHLPRLDQFPYLKYLYLRDLPNIEY--IGNDDSVPSSTIFPSFE 359
            SLGCL+ I L  C+RL+HL RLDQFP LKYL L++LPNIEY  + NDDSV SSTIFP  +
Sbjct: 850  SLGCLVTIYLYRCKRLRHLFRLDQFPNLKYLTLQNLPNIEYMIVDNDDSVSSSTIFPCLK 909

Query: 360  ELR-----------------------FPCLSVLEIHG-CPLHILMSWHAP-LKSLTIGNS 419
            +                         FP LS L I G C LH+L  WHAP LK L I +S
Sbjct: 910  KFTISKMPKLVSWCKDSTSTKSPTVIFPHLSSLMIRGPCRLHMLKYWHAPKLKLLQISDS 969

Query: 420  RNGGHCLKGDEFDVVPWKIFENLSSLGLFGMRKLEYLPECLQH-VTTLQSLTLWFCQNLK 479
                     DE +VVP KI+ENL+SL L  + ++EYLPEC QH +T+LQ L L  C+NLK
Sbjct: 970  E--------DELNVVPLKIYENLTSLFLHNLSRVEYLPECWQHYMTSLQLLYLSKCENLK 1029

Query: 480  CLPRWIGNLTSLTNFEIVCCEKLTLLPEEI-------------C-----------HIHGL 509
             LP WIGNLTSLT  +I  C+KLT+LPEEI             C           HIH L
Sbjct: 1030 SLPGWIGNLTSLTGLKISTCDKLTMLPEEIDNLTSLTNLDISYCKNLAFLPEGIQHIHNL 1089

BLAST of HG10003189 vs. NCBI nr
Match: XP_031736581.1 (putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651704.1 hypothetical protein Csa_006537 [Cucumis sativus])

HSP 1 Score: 155.6 bits (392), Expect = 1.2e-33
Identity = 99/192 (51.56%), Postives = 129/192 (67.19%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLEL-SLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSES 60
           MAE+ILFN    I+ KLGS A  +  SL  GV D+ +KL  +++AI+AVL DAE++Q + 
Sbjct: 1   MAEAILFNLTADIIFKLGSSALRQFGSLRGGVKDDFDKLWHSLSAIQAVLHDAEEKQFKD 60

Query: 61  RAVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLK 120
            AV+ WVS+LKD  Y++DDLIDEFSY+ LRRQV+    +  +KQVR  FSK        K
Sbjct: 61  HAVEVWVSRLKDVLYEIDDLIDEFSYQILRRQVL----QSNRKQVRTLFSK---FITNWK 120

Query: 121 MGHKIKEIRESLKAINDDKTQFSFSECMIDRRYD---GFRERRETGSYIPEEEVIGLERP 180
           +GHKIKEI + L+ IN+DK QFSF + +I+RR D   G R+RRET S+I E+EVIG    
Sbjct: 121 IGHKIKEISQRLQNINEDKIQFSFCKHVIERRDDDDEGLRKRRETHSFILEDEVIGRNDD 180

Query: 181 KEE----LQNSN 185
           KE     L NSN
Sbjct: 181 KEAVIDLLLNSN 185

HSP 2 Score: 313.9 bits (803), Expect = 2.6e-81
Identity = 186/379 (49.08%), Postives = 222/379 (58.58%), Query Frame = 0

Query: 180 LQNSNLVQIGRHNSGELNELNGLNKLRGSLSIQNLQFCTS-ADLQMEAKFLQLKSGLQTL 239
           LQ  N   +G    GEL EL+ L KLRG+L+I++L+F TS  D +M+ KFLQLKSGLQ L
Sbjct: 570 LQTMNRFVLGNKKGGELKELDRLTKLRGTLTIEHLEFGTSIVDHKMKGKFLQLKSGLQEL 629

Query: 240 DLQWDDLFDHEPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGCLI 299
            L               D   E V+DCLQPHSNLQ+I+I  Y GV LCNW+S   L CL+
Sbjct: 630 KLH--------------DVIYESVLDCLQPHSNLQKIYIDGYGGVNLCNWVSVKFLDCLV 689

Query: 300 KIRLDGCERLQHLPRLDQFPYLKYLYLRDLPNIEYI--GNDDSVPSSTIFPSFEELR--- 359
            IRL  CERL+HLPR DQFP LK L L DLPN+EYI   N+DSV SSTIFPS  EL    
Sbjct: 690 TIRLHDCERLRHLPRFDQFPNLKRLELCDLPNVEYIIVNNNDSVSSSTIFPSLNELEISK 749

Query: 360 --------------------FPCLSVLEIHG-CPLHILMSWHAP-LKSLTIGNSRNGGHC 419
                               FP LS L I+G  PLH+L  WHAP LK+L I +S      
Sbjct: 750 MPKLVSWCKGTTSTKSPTIIFPYLSCLRIYGRRPLHMLKFWHAPNLKTLLIFDSE----- 809

Query: 420 LKGDEFDVVPWKIFENLSSLGLFGMRKLEYLPECLQH-VTTLQSLTLWFCQNLKCLPRWI 479
              DE +VVP K +ENL+SL L  + KLEY PEC QH +T+LQ L L +C+NLK L  W 
Sbjct: 810 ---DELNVVPLKTYENLTSLSLRNLSKLEYFPECWQHCMTSLQFLYLSYCENLKSLAEWF 869

Query: 480 GNLTSLTNFEIVC------------------------CEKLTLLPEEICHIHGLVSLVIN 506
           GNLTSLT   I+                         CEKL LLPE I H+H L +L I+
Sbjct: 870 GNLTSLTQLHIISCGKLTMLPEGIGNLTSLTILVIFNCEKLALLPEGIQHLHNLQNLTID 926

BLAST of HG10003189 vs. NCBI nr
Match: TYK10413.1 (putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 305.1 bits (780), Expect = 1.2e-78
Identity = 255/792 (32.20%), Postives = 313/792 (39.52%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESR 60
           MAE+IL+N    I+ KLGS A  EL  LW V  EL KLK +++AI+AVLLDAE+QQS++ 
Sbjct: 1   MAEAILYNLTADIIFKLGSSALQELGSLWRVNSELHKLKHSLSAIQAVLLDAEEQQSKNN 60

Query: 61  AVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKM 120
            VKDWV KL+D  Y++DDLIDE SYE LRRQV+  K R+ +KQVRI FSK        K+
Sbjct: 61  QVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLA-KHRRTRKQVRILFSKFKS---NWKI 120

Query: 121 GHKIKEIRESLKAINDDKTQFSFSECMIDRR--YDGFRERRETGSYIPEEEVIGLERPKE 180
           GHKIKEIRE L+AIN+DK QF F + +I+RR  Y+G R+RRET S+I E+EVIG    KE
Sbjct: 121 GHKIKEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFILEDEVIGRNDDKE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 AVIDLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYTHYGMTNDGQFQMKLWVCVSEEFD 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 LKIIIQKIIESATGKKTESFLQLDSLQCELRKQIDGKKYLLVMDDVWNEKKEEWLHLKRL 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LMGGAKGSRILITTRSEQVAKTFDSTFVHRLQILDASNSWLLFQKMTGLEERFDNQEVKL 360

Query: 361 ELQNSNLVQI-------------------------------------------------- 420
           + +NSNL++I                                                  
Sbjct: 361 DQKNSNLIKIGMEIVSMLNGVPLVIRNIGGLLKDNKSERFWLSFKDKELHQVLRQEQDAL 420

Query: 421 ------------------------------------------------------------ 430
                                                                       
Sbjct: 421 KEMQLILELSYKYLPSSNLKQCFRYCALFPKDYKIQKNELILLWRAQGFIQPNGNNNDDG 480

BLAST of HG10003189 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 6.9e-37
Identity = 110/364 (30.22%), Postives = 173/364 (47.53%), Query Frame = 0

Query: 188 IGRHNSGELNELNGLNKLRGSLSIQNLQFCTSADLQMEAKFLQLKSGLQTLDLQWDDLFD 247
           +G     +L EL  LN LRG++SI +L+   + D++ +   L  K+ L +L + WD    
Sbjct: 638 VGERKGYQLGELRNLN-LRGAISITHLERVKN-DMEAKEANLSAKANLHSLSMSWD---- 697

Query: 248 HEPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGCLIKIRLDGCER 307
             P    S++   +V++ L+PH NL+ + I  + G  L +W++   L  ++ I + GCE 
Sbjct: 698 -RPNRYESEE--VKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCEN 757

Query: 308 LQHLPRLDQFPYLKYLYLRD-LPNIEYIGNDDSVPSSTIFPSFEEL-------------- 367
              LP   + P L+ L L+D    +EY+  D    +   FPS  +L              
Sbjct: 758 CSCLPPFGELPCLESLELQDGSVEVEYV-EDSGFLTRRRFPSLRKLHIGGFCNLKGLQRM 817

Query: 368 ----RFPCLSVLEIHGCPLHILMSWHAPLKSLTIGNSRNGGHCLKGDEFDVVPWKIF--- 427
               +FP L  ++I  CP+ +  +  +  K    G +  GG     +   +   KIF   
Sbjct: 818 KGAEQFPVLEEMKISDCPMFVFPTLSSVKKLEIWGEADAGGLSSISNLSTLTSLKIFSNH 877

Query: 428 -------------ENLSSLGLFGMRKLEYLPECLQHVTTLQSLTLWFCQNLKCLP-RWIG 487
                        ENL  L +  +  L+ LP  L  +  L+ L + +C  L+ LP   + 
Sbjct: 878 TVTSLLEEMFKNLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLE 937

Query: 488 NLTSLTNFEIVCCEKLTLLPEEICHIHGLVSLVINGCPELEEKCKKGTGEDWPKIAHIPQ 516
            L+SLT   +  C  L  LPE + H+  L SL I GCP+L ++C+KG GEDW KI+HIP 
Sbjct: 938 GLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIPN 991

HSP 2 Score: 83.2 bits (204), Expect = 9.6e-15
Identity = 59/180 (32.78%), Postives = 96/180 (53.33%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESR 60
           MAE+ L      +L  L  F   EL L++G   E +KL    + I+AVL DA+++Q + +
Sbjct: 1   MAEAFL----QVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60

Query: 61  AVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKM 120
           A+K+W+ KL  A Y+VDD++D+   E  R            KQ  +       I F  K+
Sbjct: 61  AIKNWLQKLNVAAYEVDDILDDCKTEAAR-----------FKQAVLGRYHPRTITFCYKV 120

Query: 121 GHKIKEIRESLKAINDDKTQFSFSECMIDRRYDGFRERRETGSYIPEEEVIGLERPKEEL 180
           G ++KE+ E L AI +++  F   E +I+R+      RR+TG  + E +V G E+ ++E+
Sbjct: 121 GKRMKEMMEKLDAIAEERRNFHLDERIIERQ----AARRQTGFVLTEPKVYGREKEEDEI 161

BLAST of HG10003189 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.8e-36
Identity = 110/353 (31.16%), Postives = 162/353 (45.89%), Query Frame = 0

Query: 188 IGRHNSGELNELNGLNKLRGSLSIQNLQFCTSADLQMEAKFLQLKSGLQTLDLQWDDLFD 247
           +GR    +L EL  LN L GS+ I +L+   +     EA  L  K  L +L + W++   
Sbjct: 630 VGRKKGYQLGELGNLN-LYGSIKISHLERVKNDKDAKEAN-LSAKGNLHSLSMSWNNFGP 689

Query: 248 HEPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGCLIKIRLDGCER 307
           H     I + +  +V++ L+PHSNL  + I  ++G+ L  W++   L  ++ I +     
Sbjct: 690 H-----IYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRN 749

Query: 308 LQHLPRLDQFPYLKYLYLR-DLPNIEYIGNDD-----SVPSSTIFPSFEEL--------- 367
              LP     P L+ L L     ++EY+   D       P+   FPS  +L         
Sbjct: 750 CSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSLK 809

Query: 368 ---------RFPCLSVLEIHGCPLHILMSWHAPLKSLTIGNSRNGGHCLKGDEFDVVPWK 427
                    +FP L  + IH CP   L S    L SL I  ++          F    +K
Sbjct: 810 GLLKKEGEEQFPVLEEMIIHECPFLTLSSNLRALTSLRICYNK------VATSFPEEMFK 869

Query: 428 IFENLSSLGLFGMRKLEYLPECLQHVTTLQSLTLWFCQNLKCLP-RWIGNLTSLTNFEIV 487
              NL  L +     L+ LP  L  +  L+SL +  C  L+ LP   +  L+SLT   + 
Sbjct: 870 NLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVE 929

Query: 488 CCEKLTLLPEEICHIHGLVSLVINGCPELEEKCKKGTGEDWPKIAHIPQVRIY 516
            C  L  LPE + H+  L SL I GCP+L ++C+KG GEDW KI+HIP V IY
Sbjct: 930 HCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIPNVNIY 969

HSP 2 Score: 88.2 bits (217), Expect = 3.0e-16
Identity = 54/168 (32.14%), Postives = 97/168 (57.74%), Query Frame = 0

Query: 13  ILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESRAVKDWVSKLKDA 72
           +L  L SF   EL LL+G  DE ++L    + I+AVL DA+++Q  ++ +++W+ KL  A
Sbjct: 9   LLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAA 68

Query: 73  FYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKMGHKIKEIRESLK 132
            Y+VDD++DE+  +  R      +   G+   ++       I FR K+G ++ ++ + LK
Sbjct: 69  TYEVDDILDEYKTKATR----FSQSEYGRYHPKV-------IPFRHKVGKRMDQVMKKLK 128

Query: 133 AINDDKTQFSFSECMIDRRYDGFRERRETGSYIPEEEVIGLERPKEEL 181
           AI +++  F   E +++R+      RRETGS + E +V G ++ K+E+
Sbjct: 129 AIAEERKNFHLHEKIVERQ----AVRRETGSVLTEPQVYGRDKEKDEI 161

BLAST of HG10003189 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 2.9e-35
Identity = 111/372 (29.84%), Postives = 176/372 (47.31%), Query Frame = 0

Query: 180 LQNSNLVQIGRHNSGELNELNGLNKLRGSLSIQNLQFCTSADLQMEAKFLQLKSGLQTLD 239
           L++ +   IG+    +L EL  LN L GS+SI  L      D   +   L  K+ L +L 
Sbjct: 621 LKSLSCFVIGKRKGHQLGELKNLN-LYGSISITKLD-RVKKDTDAKEANLSAKANLHSLC 680

Query: 240 LQWDDLFDHEPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGCLIK 299
           L WD   D +   D        V++ L+PHSNL+ + I  + G++L +W++   L  ++ 
Sbjct: 681 LSWD--LDGKHRYD------SEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVS 740

Query: 300 IRLDGCERLQHLPRLDQFPYLKYLYLR-DLPNIEYIGNDDSVPSSTIFPSFEEL------ 359
           IR+ GCE    LP   + P L+ L L     ++EY+  +D+V     FPS  +L      
Sbjct: 741 IRIRGCENCSCLPPFGELPCLESLELHTGSADVEYV--EDNVHPGR-FPSLRKLVIWDFS 800

Query: 360 ------------RFPCLSVLEIHGCP------------LHILMSWHAPLKSLTIGNSRNG 419
                       +FP L  +  + CP            L ++++    L+S++   +   
Sbjct: 801 NLKGLLKMEGEKQFPVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRSISNLRALTS 860

Query: 420 GHCLKGDEFDVVPWKIFE---NLSSLGLFGMRKLEYLPECLQHVTTLQSLTLWFCQNLKC 479
                  E   +P ++F+   NL  L +   R L+ LP  L  +  L+SL   FC  L+ 
Sbjct: 861 LDISDNVEATSLPEEMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALES 920

Query: 480 LP-RWIGNLTSLTNFEIVCCEKLTLLPEEICHIHGLVSLVINGCPELEEKCKKGTGEDWP 517
           LP   +  LTSLT   +  C  L  LPE + H+  L +L I  CP + ++C++G GEDW 
Sbjct: 921 LPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQCPIVFKRCERGIGEDWH 979

HSP 2 Score: 84.7 bits (208), Expect = 3.3e-15
Identity = 53/168 (31.55%), Postives = 95/168 (56.55%), Query Frame = 0

Query: 13  ILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESRAVKDWVSKLKDA 72
           +L  L SF   EL LL+G  DE ++L    + I+AVL DA+++Q   + +++W+ KL  A
Sbjct: 9   VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68

Query: 73  FYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKMGHKIKEIRESLK 132
            Y+VDD++DE+  +  R      +   G+   ++       I FR K+G ++ ++ + L 
Sbjct: 69  TYEVDDILDEYKTKATR----FLQSEYGRYHPKV-------IPFRHKVGKRMDQVMKKLN 128

Query: 133 AINDDKTQFSFSECMIDRRYDGFRERRETGSYIPEEEVIGLERPKEEL 181
           AI +++ +F   E +I+R+       RETGS + E +V G ++ K+E+
Sbjct: 129 AIAEERKKFHLQEKIIERQ----AATRETGSVLTEPQVYGRDKEKDEI 161

BLAST of HG10003189 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.9e-34
Identity = 108/367 (29.43%), Postives = 172/367 (46.87%), Query Frame = 0

Query: 188 IGRHNSGELNELNGLNKLRGSLSIQNLQFCTSADLQMEAKFLQLKSGLQTLDLQWDDLFD 247
           +G     +L EL  LN L GS+SI +L+   + D   EA  L  K+ LQ+L + WD   +
Sbjct: 630 VGSKKGYQLGELKNLN-LCGSISITHLERVKN-DTDAEAN-LSAKANLQSLSMSWD---N 689

Query: 248 HEPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGCLIKIRLDGCER 307
             P    S +   +V++ L+PH NL+ + I  + G +  +W++   L  +I +R+  C+ 
Sbjct: 690 DGPNRYESKE--VKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKN 749

Query: 308 LQHLPRLDQFPYLKYLYLRD-LPNIEYIGNDD---SVPSSTIFPSFEELR---------- 367
              LP   + P L+ L L++    +EY+  DD      +   FPS ++LR          
Sbjct: 750 CLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSLKGL 809

Query: 368 --------FPCLSVLEIHGCPLHILMSWHAPLKSLTIGNSRNGGHCLKGDEFDVVPWKI- 427
                   FP L  + I  CPL +  +  +  K    GN+   G     +   +   +I 
Sbjct: 810 MKEEGEEKFPMLEEMAILYCPLFVFPTLSSVKKLEVHGNTNTRGLSSISNLSTLTSLRIG 869

Query: 428 ---------------FENLSSLGLFGMRKLEYLPECLQHVTTLQSLTLWFCQNLKCLP-R 487
                            NL  L  F  + L+ LP  L  +  L+ L +  C +L+  P +
Sbjct: 870 ANYRATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSLTSLNALKRLQIESCDSLESFPEQ 929

Query: 488 WIGNLTSLTNFEIVCCEKLTLLPEEICHIHGLVSLVINGCPELEEKCKKGTGEDWPKIAH 516
            +  LTSLT   +  C+ L  LPE + H+  L +L ++GCPE+E++C K  GEDW KIAH
Sbjct: 930 GLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSGCPEVEKRCDKEIGEDWHKIAH 988

HSP 2 Score: 92.0 bits (227), Expect = 2.1e-17
Identity = 61/180 (33.89%), Postives = 101/180 (56.11%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESR 60
           MAE+ L      +L  L SF   +L L++G   E EKL    + I+AVL DA+++Q + +
Sbjct: 1   MAEAFL----QVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60

Query: 61  AVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKM 120
           A+++W+ KL  A Y+VDD++ E   E +R +           Q R+ F     I FR K+
Sbjct: 61  AIENWLQKLNSAAYEVDDILGECKNEAIRFE-----------QSRLGFYHPGIINFRHKI 120

Query: 121 GHKIKEIRESLKAINDDKTQFSFSECMIDRRYDGFRERRETGSYIPEEEVIGLERPKEEL 180
           G ++KEI E L AI++++ +F F E + +R+       RETG  + E +V G ++ ++E+
Sbjct: 121 GRRMKEIMEKLDAISEERRKFHFLEKITERQ--AAAATRETGFVLTEPKVYGRDKEEDEI 163

BLAST of HG10003189 vs. ExPASy Swiss-Prot
Match: Q9SX38 (Putative disease resistance protein At1g50180 OS=Arabidopsis thaliana OX=3702 GN=At1g50180 PE=3 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 1.4e-13
Identity = 55/185 (29.73%), Postives = 97/185 (52.43%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESR 60
           MAE+I+  T    + KLG     E   L+G+GD++++L+D +  +   L DA+++Q ES 
Sbjct: 1   MAEAIVSVT----VQKLGQLLLEEPLFLFGIGDQVKQLQDELKRLNCFLKDADEKQHESE 60

Query: 61  AVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKM 120
            V++WV+ +++A YD +D+++ F  +   R     K++  K+ +R      N+      +
Sbjct: 61  RVRNWVAGIREASYDAEDILEAFFLKAESR-----KQKGMKRVLRRLACILNEAVSLHSV 120

Query: 121 GHKIKEIRESLKAINDDKTQFSFSECMIDRRY---DGFRERRETGSYIPEEEVIGLERPK 180
           G +I+EI   L  I      F   E M        D  RE+R++  Y+ E  ++GLE+  
Sbjct: 121 GSEIREITSRLSKIAASMLDFGIKESMGREGLSLSDSLREQRQSFPYVVEHNLVGLEQSL 176

Query: 181 EELQN 183
           E+L N
Sbjct: 181 EKLVN 176

BLAST of HG10003189 vs. ExPASy TrEMBL
Match: A0A1S4E5A9 (putative disease resistance protein RGA1 OS=Cucumis melo OX=3656 GN=LOC103503170 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 1.2e-92
Identity = 198/369 (53.66%), Postives = 236/369 (63.96%), Query Frame = 0

Query: 180 LQNSNLVQIGRHNSGELNELNGLNKLRGSLSIQNLQFCTS-ADLQMEAKFLQLKSGLQTL 239
           LQ  N   +G++   EL EL+GL KLRG LSI++L FC+S  D QM+ KFLQ KS LQ L
Sbjct: 301 LQTMNTFVLGKNKGSELKELDGLTKLRGELSIRHLAFCSSIVDQQMKGKFLQQKSDLQVL 360

Query: 240 DLQWDD--LFDHEPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGC 299
           DL W+   + D E    + D   E V+DCLQPHSNL++I+I  Y GVKLCNW+SS  LGC
Sbjct: 361 DLNWEKHLIVDDE----LEDVIYESVLDCLQPHSNLRKIYINGYGGVKLCNWVSSKFLGC 420

Query: 300 LIKIRLDGCERLQHLPRLDQFPYLKYLYLRDLPNIEYI--GNDDSVPSSTIFPSFEELR- 359
           L+ +RL  CERL+HLPR DQFP LK L L  LPNIEYI   N+DSV SST FPS +EL  
Sbjct: 421 LVTVRLYHCERLRHLPRFDQFPNLKRLSLEVLPNIEYIIVNNNDSVSSSTNFPSMKELEI 480

Query: 360 ----------------------FPCLSVLEIHG-CPLHILMSWHAP-LKSLTIGNSRNGG 419
                                 FPCLS L I+G CPLH+L  WHAP LKSL I +S    
Sbjct: 481 SNMPKLVSWCKGTTLAKSPIIIFPCLSSLTINGRCPLHMLKFWHAPNLKSLKISDSE--- 540

Query: 420 HCLKGDEFDVVPWKIFENLSSLGLFGMRKLEYLPECLQH-VTTLQSLTLWFCQNLKCLPR 479
                DE +VVP K +ENL+SL LF + +LEYLPEC QH +T+L+ L L  C+NLK LP 
Sbjct: 541 -----DELNVVPLKNYENLTSLCLFNLSELEYLPECWQHCMTSLRFLCLGRCKNLKSLPE 600

Query: 480 WIGNLTSLTNFEIVCCEKLTLLPEEICHIHGLVSLVINGCPELEEKCKKGTGEDWPKIAH 518
           WI N TSL   +I  CE LTLLPE I HI  L  L I  CP LEE+CK+  GEDW KIAH
Sbjct: 601 WIDNFTSLDRLDIFSCENLTLLPEGIQHIRNLQRLRIECCPVLEERCKENIGEDWHKIAH 657

BLAST of HG10003189 vs. ExPASy TrEMBL
Match: A0A0A0LMP4 (Rx_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G074130 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 1.8e-88
Identity = 298/921 (32.36%), Postives = 364/921 (39.52%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLEL-SLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSES 60
           MAE+ILFN    I+ KLGS A  +  SL  GV D+ +KL  +++AI+AVL DAE++Q + 
Sbjct: 1   MAEAILFNLTADIIFKLGSSALRQFGSLRGGVKDDFDKLWHSLSAIQAVLHDAEEKQFKD 60

Query: 61  RAVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLK 120
            AV+ WVS+LKD  Y++DDLIDEFSY+ LRRQV+    +  +KQVR  FSK        K
Sbjct: 61  HAVEVWVSRLKDVLYEIDDLIDEFSYQILRRQVL----QSNRKQVRTLFSK---FITNWK 120

Query: 121 MGHKIKEIRESLKAINDDKTQFSFSECMIDRRYD---GFRERRETGSYIPEEEVIGLERP 180
           +GHKIKEI + L+ IN+DK QFSF + +I+RR D   G R+RRET S+I E+EVIG    
Sbjct: 121 IGHKIKEISQRLQNINEDKIQFSFCKHVIERRDDDDEGLRKRRETHSFILEDEVIGRNDD 180

Query: 181 KE------------------------------------ELQ------------------- 240
           KE                                    E+Q                   
Sbjct: 181 KEAVIDLLLNSNTKEDIAIVSINKELYQVLGRGQDALKEIQLFLELSYKYLPSSNLKQCF 240

Query: 241 -----------------------------------NSNLVQIGR---------------- 300
                                              NS+LV IG                 
Sbjct: 241 LYCALFPKDYRIKKDELILLWRAQGFIQQNGNNDDNSSLVDIGEDYFMELLSRSFFQEVE 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 KNDFGDIITCKMHDLMHDLACSITNNECVRGLKGNVIDKRTHHLSFEKVSHEDQLMGSLS 360

Query: 361 -----------------------HN----------------------------------- 420
                                  HN                                   
Sbjct: 361 KATHLRTLFIQDVCSRCNLEETFHNIFQLRTLHLNLYSPTKFAKTWKFISKLKHLRYLHL 420

Query: 421 ------------------------------------------------------------ 480
                                                                       
Sbjct: 421 KNSFCVTYLPDSILELYNLETFIFQSSLLKKLPSNVGNLINLKHLDLSSHLNLEFLPDSI 480

Query: 481 ------------------------------------------------------------ 509
                                                                       
Sbjct: 481 TKLYKLEALILHGCSNLKELPKYTKRLINLKSLVLYGCSALTHMPKGLSEMTNLQTLTTF 540

BLAST of HG10003189 vs. ExPASy TrEMBL
Match: A0A6J1DKY9 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111021874 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.1e-85
Identity = 190/382 (49.74%), Postives = 226/382 (59.16%), Query Frame = 0

Query: 191  HNSGELNELNGLNKLRGSLSIQNLQFCTSADLQMEAKFLQLKSGLQ--TLDLQWDDLFDH 250
            +N G L+ELN LNKLRGSL+I  L FCT++DL   AKFL LKS LQ   L+ +WD+   +
Sbjct: 788  NNGGGLSELNELNKLRGSLTINRLNFCTTSDLPTNAKFLLLKSSLQELVLEWEWDNDSVN 847

Query: 251  EPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGCLIKIRLDGCERL 310
            EPT    DDDCE V++CL PHSNL+ + I +Y+GVKLCNW+SS+ LGCL+ I+L GC+RL
Sbjct: 848  EPTIGDLDDDCESVLECLHPHSNLRNLCIDKYRGVKLCNWVSSNFLGCLVTIKLSGCKRL 907

Query: 311  QHLPRLDQFPYLKYLYLRDLPNIEYIGNDDSVPSSTIFPSFEELR--------------- 370
            QHLP+ DQFP LK L LR+L  IEYI NDD   SST+FPS EEL                
Sbjct: 908  QHLPQFDQFPNLKRLSLRELSGIEYIFNDDYRCSSTLFPSLEELSIFCMPNLKRWWKKGQ 967

Query: 371  ---------------FPCLSVLEIHGCPLHILMSWHAPLKSLTIGNSRNGGHCLKGDEFD 430
                           FPCLS LEI GCPL   ++ HAP KSL + +          DE D
Sbjct: 968  TSKATSSQNVLFPIIFPCLSKLEIWGCPLLASITCHAPPKSLEMNDPIE--FHFDPDESD 1027

Query: 431  VVPWKIFENLSSLGLFGMRKLEYLPECLQHVTTLQSLTLWFCQN---------------- 490
            VV WK FE+LS L L  + +  +L   LQHVTTLQ LTL +CQN                
Sbjct: 1028 VVSWKCFESLSYLRLTHIDEWGFLSNWLQHVTTLQELTLEYCQNLTNLPESISNFTSLSK 1087

Query: 491  ---LKC-----LPRWIGNLTSLTNFEIVCCEKLTLLPEEICHIHGLVSLVINGCPELEEK 517
               LKC     LP  IGNLTSLT   I  C  LT LPE I H+H L SL    CP LE+ 
Sbjct: 1088 LEILKCTDLMSLPESIGNLTSLTELTIDFCPSLTSLPEGIRHLHCLQSLTTWDCPILEKM 1147

BLAST of HG10003189 vs. ExPASy TrEMBL
Match: A0A6J1DKY9 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111021874 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.3e-51
Identity = 121/189 (64.02%), Postives = 141/189 (74.60%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESR 60
           MA+SILFN AGT++ KLG +   EL LL GV  EL+KLK TV+AI  VLLDAE++ S+S 
Sbjct: 1   MADSILFNLAGTLINKLGXYVHQELGLLLGVNGELDKLKGTVSAIXKVLLDAEERSSKSH 60

Query: 61  AVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKM 120
           A++DW+SKLK  FY++DDLIDEF+YE LRRQVMT   RK  KQVRIFFSKSNQIAF  KM
Sbjct: 61  ALEDWLSKLKGVFYEIDDLIDEFNYEALRRQVMTRNRRK-TKQVRIFFSKSNQIAFSHKM 120

Query: 121 GHKIKEIRESLKAINDDKTQFSFSECMIDRR-YDGFRERRETGSYIPEEEVIGLERPKEE 180
           G KIKEIRE LK INDDKTQF  SEC+I++   D  R  R+ G YIPEEEVIG    K+E
Sbjct: 121 GGKIKEIREKLKVINDDKTQFKLSECVINKSDVDILRNTRDPGDYIPEEEVIGRNYEKKE 180

Query: 181 ----LQNSN 185
               L NSN
Sbjct: 181 IIDLLLNSN 188

HSP 2 Score: 313.9 bits (803), Expect = 1.2e-81
Identity = 186/379 (49.08%), Postives = 222/379 (58.58%), Query Frame = 0

Query: 180 LQNSNLVQIGRHNSGELNELNGLNKLRGSLSIQNLQFCTS-ADLQMEAKFLQLKSGLQTL 239
           LQ  N   +G    GEL EL+ L KLRG+L+I++L+F TS  D +M+ KFLQLKSGLQ L
Sbjct: 570 LQTMNRFVLGNKKGGELKELDRLTKLRGTLTIEHLEFGTSIVDHKMKGKFLQLKSGLQEL 629

Query: 240 DLQWDDLFDHEPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSLGCLI 299
            L               D   E V+DCLQPHSNLQ+I+I  Y GV LCNW+S   L CL+
Sbjct: 630 KLH--------------DVIYESVLDCLQPHSNLQKIYIDGYGGVNLCNWVSVKFLDCLV 689

Query: 300 KIRLDGCERLQHLPRLDQFPYLKYLYLRDLPNIEYI--GNDDSVPSSTIFPSFEELR--- 359
            IRL  CERL+HLPR DQFP LK L L DLPN+EYI   N+DSV SSTIFPS  EL    
Sbjct: 690 TIRLHDCERLRHLPRFDQFPNLKRLELCDLPNVEYIIVNNNDSVSSSTIFPSLNELEISK 749

Query: 360 --------------------FPCLSVLEIHG-CPLHILMSWHAP-LKSLTIGNSRNGGHC 419
                               FP LS L I+G  PLH+L  WHAP LK+L I +S      
Sbjct: 750 MPKLVSWCKGTTSTKSPTIIFPYLSCLRIYGRRPLHMLKFWHAPNLKTLLIFDSE----- 809

Query: 420 LKGDEFDVVPWKIFENLSSLGLFGMRKLEYLPECLQH-VTTLQSLTLWFCQNLKCLPRWI 479
              DE +VVP K +ENL+SL L  + KLEY PEC QH +T+LQ L L +C+NLK L  W 
Sbjct: 810 ---DELNVVPLKTYENLTSLSLRNLSKLEYFPECWQHCMTSLQFLYLSYCENLKSLAEWF 869

Query: 480 GNLTSLTNFEIVC------------------------CEKLTLLPEEICHIHGLVSLVIN 506
           GNLTSLT   I+                         CEKL LLPE I H+H L +L I+
Sbjct: 870 GNLTSLTQLHIISCGKLTMLPEGIGNLTSLTILVIFNCEKLALLPEGIQHLHNLQNLTID 926

BLAST of HG10003189 vs. ExPASy TrEMBL
Match: A0A5D3CEX2 (Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00570 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 5.8e-79
Identity = 255/792 (32.20%), Postives = 313/792 (39.52%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESR 60
           MAE+IL+N    I+ KLGS A  EL  LW V  EL KLK +++AI+AVLLDAE+QQS++ 
Sbjct: 1   MAEAILYNLTADIIFKLGSSALQELGSLWRVNSELHKLKHSLSAIQAVLLDAEEQQSKNN 60

Query: 61  AVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKM 120
            VKDWV KL+D  Y++DDLIDE SYE LRRQV+  K R+ +KQVRI FSK        K+
Sbjct: 61  QVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLA-KHRRTRKQVRILFSKFKS---NWKI 120

Query: 121 GHKIKEIRESLKAINDDKTQFSFSECMIDRR--YDGFRERRETGSYIPEEEVIGLERPKE 180
           GHKIKEIRE L+AIN+DK QF F + +I+RR  Y+G R+RRET S+I E+EVIG    KE
Sbjct: 121 GHKIKEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFILEDEVIGRNDDKE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 AVIDLLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYTHYGMTNDGQFQMKLWVCVSEEFD 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 LKIIIQKIIESATGKKTESFLQLDSLQCELRKQIDGKKYLLVMDDVWNEKKEEWLHLKRL 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LMGGAKGSRILITTRSEQVAKTFDSTFVHRLQILDASNSWLLFQKMTGLEERFDNQEVKL 360

Query: 361 ELQNSNLVQI-------------------------------------------------- 420
           + +NSNL++I                                                  
Sbjct: 361 DQKNSNLIKIGMEIVSMLNGVPLVIRNIGGLLKDNKSERFWLSFKDKELHQVLRQEQDAL 420

Query: 421 ------------------------------------------------------------ 430
                                                                       
Sbjct: 421 KEMQLILELSYKYLPSSNLKQCFRYCALFPKDYKIQKNELILLWRAQGFIQPNGNNNDDG 480

BLAST of HG10003189 vs. TAIR 10
Match: AT1G50180.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 79.3 bits (194), Expect = 9.9e-15
Identity = 55/185 (29.73%), Postives = 97/185 (52.43%), Query Frame = 0

Query: 1   MAESILFNTAGTILTKLGSFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSESR 60
           MAE+I+  T    + KLG     E   L+G+GD++++L+D +  +   L DA+++Q ES 
Sbjct: 1   MAEAIVSVT----VQKLGQLLLEEPLFLFGIGDQVKQLQDELKRLNCFLKDADEKQHESE 60

Query: 61  AVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMTPKERKGKKQVRIFFSKSNQIAFRLKM 120
            V++WV+ +++A YD +D+++ F  +   R     K++  K+ +R      N+      +
Sbjct: 61  RVRNWVAGIREASYDAEDILEAFFLKAESR-----KQKGMKRVLRRLACILNEAVSLHSV 120

Query: 121 GHKIKEIRESLKAINDDKTQFSFSECMIDRRY---DGFRERRETGSYIPEEEVIGLERPK 180
           G +I+EI   L  I      F   E M        D  RE+R++  Y+ E  ++GLE+  
Sbjct: 121 GSEIREITSRLSKIAASMLDFGIKESMGREGLSLSDSLREQRQSFPYVVEHNLVGLEQSL 176

Query: 181 EELQN 183
           E+L N
Sbjct: 181 EKLVN 176

BLAST of HG10003189 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 78.6 bits (192), Expect = 1.7e-14
Identity = 88/338 (26.04%), Postives = 141/338 (41.72%), Query Frame = 0

Query: 178 EELQNSNLVQIGRHNSGELNELNGLNKLRGSLSIQNLQFCTSADLQMEAKFLQLKSGLQT 237
           + LQ      +   +   ++EL GL+ L G L I  LQ         EA  L  K  L+ 
Sbjct: 672 KSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEAN-LNSKKHLRE 731

Query: 238 LDLQW---DDLFDHEPTTDISDDDCERVMDCLQPHSNLQEIFIRRYKGVKLCNWLSSDSL 297
           +D  W       ++      + ++ E V + L+PH +++++ I RYKG +  +WLS  S 
Sbjct: 732 IDFVWRTGSSSSENNTNPHRTQNEAE-VFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSF 791

Query: 298 GCLIKIRLDGCERLQHLPRLDQFPYLKYLYLRDLPNIEYIG-----------NDDSVPSS 357
             ++ IRL  C+    LP L Q P LK L++  +  ++ IG           + D  P  
Sbjct: 792 SRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQP-- 851

Query: 358 TIFPSFEELRFPCL----SVLEIHGCPLHILMSWHAPLKSLTIGNSRNGGHCLKGDEFDV 417
             F S E LRF  L      L++      +  S    LK L I         L+  E   
Sbjct: 852 --FRSLETLRFDNLPDWQEWLDVRVTRGDLFPS----LKKLFI---------LRCPELTG 911

Query: 418 VPWKIFENLSSLGLFGMRKLEYLPECLQH-VTTLQSLTL-WFCQNLKCLPRWIGNLTSLT 477
                  +L SL ++    L++ P+  ++    LQ+L++   C  L   P  + +  +L 
Sbjct: 912 TLPTFLPSLISLHIYKCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKFP--LNHFANLD 971

Query: 478 NFEIVCCEKLTLLPEEICHIHG---LVSLVINGCPELE 493
             E+  C  L  L     H+ G   L +L IN C  L+
Sbjct: 972 KLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQ 988

HSP 2 Score: 52.4 bits (124), Expect = 1.3e-06
Identity = 44/166 (26.51%), Postives = 75/166 (45.18%), Query Frame = 0

Query: 35  LEKLKDTVTAIEAVLLDAEQQQSESRAVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVMT 94
           LE+L   +  I AVL+DAE++Q  +  V+ WV++L+D  Y  +D +D+ + E LR  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNI-- 98

Query: 95  PKERKGKKQVRIFFSKSNQIAFRLKMGHKIKEIRESLKAINDDKTQFSFSECMIDRRYDG 154
             E     ++R       Q+  R+ +G  +    E L+    +K           R   G
Sbjct: 99  GAESSSSNRLR-------QLRGRMSLGDFLDGNSEHLET-RLEKVTIRLERLASQRNILG 158

Query: 155 FRE--------RRETGSYIPEEEVIGLERPKEELQNSNLVQIGRHN 193
            +E        R  T S + E EV G +  K+E+    + + G+ N
Sbjct: 159 LKELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDN 194

BLAST of HG10003189 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 69.3 bits (168), Expect = 1.0e-11
Identity = 63/220 (28.64%), Postives = 92/220 (41.82%), Query Frame = 0

Query: 172 GLERPKEELQNSNLVQIGRHNSGELNELNGLNKLRGSLSIQNLQFCTSADLQMEAKFLQL 231
           G+++ +   + SN V IGR +   L+EL  L+ LRG+L I  LQ    A    +A  L+ 
Sbjct: 661 GIKKLRSLQKLSNFV-IGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAG-LKR 720

Query: 232 KSGLQTLDLQWDDLFDHEPTTDISDDDCER--VMDCLQPHSNLQEIFIRRYKGVKLCNWL 291
           K  L  L L+W            +   C++  V+  L+PH +L+   I  Y+G     WL
Sbjct: 721 KPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWL 780

Query: 292 SSDSLGCLIKIRLDGCERLQHLPRLDQFPYLKYLYLRDLPNIEYIG--------NDDSVP 351
              S   +  + L  C     LP + Q P LKYL +     ++ +G        N   VP
Sbjct: 781 GDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVP 840

Query: 352 SST---------------IFPSFEELRFPCLSVLEIHGCP 367
             +               I P  E+  FPCL  L I  CP
Sbjct: 841 FQSLQILKFYGMPRWDEWICPELEDGIFPCLQKLIIQRCP 878

HSP 2 Score: 52.4 bits (124), Expect = 1.3e-06
Identity = 32/94 (34.04%), Postives = 52/94 (55.32%), Query Frame = 0

Query: 1  MAESILFNTAGTILTKLG-SFACLELSLLWGVGDELEKLKDTVTAIEAVLLDAEQQQSES 60
          MA S L + A  ++ ++  S   +EL         L++LK  +     VL DA+Q+    
Sbjct: 1  MANSYLSSCANVMVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHV 60

Query: 61 RAVKDWVSKLKDAFYDVDDLIDEFSYEKLRRQVM 94
          R VK W++ +KDAF+  +D++DE   E LRR+V+
Sbjct: 61 REVKHWLTGIKDAFFQAEDILDELQTEALRRRVV 94

BLAST of HG10003189 vs. TAIR 10
Match: AT5G44510.1 (target of AVRB operation1 )

HSP 1 Score: 66.6 bits (161), Expect = 6.6e-11
Identity = 65/237 (27.43%), Postives = 105/237 (44.30%), Query Frame = 0

Query: 270 SNLQEIFIRRYKG-VKLCNWLSSDSLGCLIKIRLDGCERLQHLP-RLDQFPYLKYLYLRD 329
           +NL+++F+ R    VKL +  S  ++  L ++ L GC  L  +P  +     LK +Y   
Sbjct: 728 TNLKKLFLNRCSSLVKLPS--SFGNVTSLKELNLSGCSSLLEIPSSIGNIVNLKKVYADG 787

Query: 330 LPNIEYIGNDDSVPSSTIFPSFEELRFPCLSVLEIHGCPLHI-------LMSWHAPLKSL 389
             ++  +    S+ ++T       L   C S++E     L++       L    + +K  
Sbjct: 788 CSSLVQL--PSSIGNNTNLKELHLLN--CSSLMECPSSMLNLTRLEDLNLSGCLSLVKLP 847

Query: 390 TIGNSRNGGHCLKGD--EFDVVPWKI--FENLSSLGLFGMRKLEYLPECLQHVTTLQSLT 449
           +IGN  N       D      +P+ I    NL +L L G   L  LP  + ++T LQSL 
Sbjct: 848 SIGNVINLQSLYLSDCSSLMELPFTIENATNLDTLYLDGCSNLLELPSSIWNITNLQSLY 907

Query: 450 LWFCQNLKCLPRWIGNLTSLTNFEIVCCEKLTLLPEEICHIHGLVSLVINGCPELEE 494
           L  C +LK LP  + N  +L +  ++ C  L  LP  I  I  L  L ++ C  L E
Sbjct: 908 LNGCSSLKELPSLVENAINLQSLSLMKCSSLVELPSSIWRISNLSYLDVSNCSSLLE 958

BLAST of HG10003189 vs. TAIR 10
Match: AT5G47280.1 (ADR1-like 3 )

HSP 1 Score: 53.5 bits (127), Expect = 5.8e-07
Identity = 30/95 (31.58%), Postives = 49/95 (51.58%), Query Frame = 0

Query: 403 KIFENLSSLGLFGMRKLEYLPECLQHVTTLQSLTLWFCQNLKCLPRWIGNLTSLTNFEIV 462
           +IF  L+ + +     L  LP  +  +T+L S+++  C N+K LP+ I  L +L    + 
Sbjct: 460 QIFPKLTDITIDYCDDLAELPSTICGITSLNSISITNCPNIKELPKNISKLQALQLLRLY 519

Query: 463 CCEKLTLLPEEICHIHGLVSLVINGCPELEEKCKK 498
            C +L  LP EIC +  LV + I+ C  L    +K
Sbjct: 520 ACPELKSLPVEICELPRLVYVDISHCLSLSSLPEK 554